AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0753610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753610 MtrunA17_Chr5g0397981 94.346 283 5 2 1 272 19 301 0.0 550
Msa0753610 MtrunA17_Chr4g0057281 73.507 268 58 3 17 272 20 286 6.61e-148 415
Msa0753610 MtrunA17_Chr8g0350401 56.929 267 98 3 17 267 23 288 8.22e-110 318
Msa0753610 MtrunA17_Chr1g0203751 55.985 259 102 3 17 264 29 286 3.22e-105 307
Msa0753610 MtrunA17_Chr6g0472801 55.078 256 103 3 18 262 24 278 2.50e-103 302
Msa0753610 MtrunA17_Chr7g0265551 53.952 291 108 5 1 266 73 362 3.32e-102 302
Msa0753610 MtrunA17_Chr7g0272101 22.993 274 167 9 19 264 4 261 2.42e-22 93.2
Msa0753610 MtrunA17_Chr4g0010881 23.864 264 155 9 19 263 4 240 8.25e-19 83.2
Msa0753610 MtrunA17_Chr1g0189671 32.639 144 84 4 18 150 6 147 5.82e-17 78.6
Msa0753610 MtrunA17_Chr1g0189611 32.192 146 85 3 18 150 6 150 7.66e-17 78.2
Msa0753610 MtrunA17_Chr1g0189591 31.293 147 80 5 18 147 15 157 7.92e-16 75.9
Msa0753610 MtrunA17_Chr7g0272091 22.759 290 174 10 11 259 2 282 3.28e-15 73.9
Msa0753610 MtrunA17_Chr1g0189641 31.507 146 83 5 18 150 6 147 3.76e-14 70.9
Msa0753610 MtrunA17_Chr1g0189751 30.667 150 79 4 18 150 6 147 1.04e-13 69.7
Msa0753610 MtrunA17_Chr1g0189721 24.427 262 175 6 18 259 6 264 2.86e-13 68.2
Msa0753610 MtrunA17_Chr1g0189621 24.046 262 175 8 18 259 6 263 4.13e-13 67.8
Msa0753610 MtrunA17_Chr1g0192371 25.316 158 104 3 19 162 11 168 7.54e-13 67.0
Msa0753610 MtrunA17_Chr1g0189781 28.378 148 87 4 19 150 11 155 8.92e-13 66.6
Msa0753610 MtrunA17_Chr1g0189821 24.638 276 162 11 18 261 6 267 1.45e-12 66.2
Msa0753610 MtrunA17_Chr1g0203281 28.472 144 90 4 18 150 6 147 5.45e-12 64.7
Msa0753610 MtrunA17_Chr1g0190071 28.082 146 92 2 18 150 6 151 1.07e-11 63.9
Msa0753610 MtrunA17_Chr1g0189771 26.174 149 89 3 19 150 15 159 5.26e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753610 AT1G12710.1 64.729 258 79 3 17 263 29 285 1.48e-119 344
Msa0753610 AT1G12710.2 55.667 300 79 4 17 263 29 327 1.73e-110 322
Msa0753610 AT3G61060.1 58.301 259 95 4 18 264 27 284 5.85e-109 317
Msa0753610 AT3G61060.2 58.077 260 95 4 18 264 27 285 6.75e-109 317
Msa0753610 AT1G63090.1 57.042 284 100 4 1 263 1 283 6.71e-105 306
Msa0753610 AT3G53000.1 50.175 285 115 4 1 259 1 284 7.65e-92 274
Msa0753610 AT5G52120.1 48.669 263 117 3 18 263 23 284 1.27e-89 268
Msa0753610 AT1G09155.1 23.843 281 178 7 19 263 3 283 7.53e-19 84.3
Msa0753610 AT2G02360.1 31.724 145 79 4 19 150 17 154 2.22e-17 80.1
Msa0753610 AT2G02230.1 29.940 167 97 7 19 170 35 196 1.82e-16 78.6
Msa0753610 AT2G02240.1 31.915 141 80 4 19 148 64 199 1.45e-15 75.9
Msa0753610 AT2G02340.1 29.651 172 101 6 6 162 26 192 1.60e-15 75.5
Msa0753610 AT2G02250.1 28.873 142 82 4 19 147 50 185 2.90e-15 74.7
Msa0753610 AT1G80110.1 23.846 260 171 8 19 259 4 255 1.76e-14 71.6
Msa0753610 AT2G02350.2 31.613 155 85 6 4 144 12 159 1.23e-13 70.1
Msa0753610 AT5G24560.1 25.150 167 104 5 18 173 5 161 3.62e-13 67.0
Msa0753610 AT1G56250.1 23.549 293 156 9 19 259 4 280 4.85e-13 68.2
Msa0753610 AT2G02320.1 28.916 166 88 6 15 162 39 192 8.02e-13 67.8
Msa0753610 AT1G56240.1 23.050 282 179 5 19 263 4 284 8.08e-13 67.4
Msa0753610 AT2G02310.1 28.966 145 83 5 18 149 47 184 6.59e-12 65.1

Find 80 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 5_2:+7239020 Msa0753610:CDS
AATTTCTTCTGTTAATTAAA+TGG 0.179440 5_2:+7241512 Msa0753610:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 5_2:-7239010 None:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 5_2:+7241344 Msa0753610:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 5_2:-7238862 None:intergenic
TAAACTTCAGATATAGTCTT+AGG 0.256782 5_2:-7241632 None:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 5_2:+7238950 Msa0753610:CDS
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 5_2:-7240991 None:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 5_2:+7238951 Msa0753610:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 5_2:+7241272 Msa0753610:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 5_2:+7239021 Msa0753610:CDS
AGCCTGTGCGATTCCAACTT+TGG 0.369551 5_2:+7241103 Msa0753610:CDS
CGCTTCAATCATCGGTTACA+TGG 0.370713 5_2:+7238876 Msa0753610:CDS
GGATTGATGATAGGAGATAT+TGG 0.372398 5_2:+7239825 Msa0753610:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 5_2:+7241160 Msa0753610:CDS
TCTTTCTACCTGAAATGGTT+AGG 0.406400 5_2:-7241352 None:intergenic
AACAAAGGACTGTCAGTACT+TGG 0.418241 5_2:+7239803 Msa0753610:CDS
TTATTCTTCAGAATACATCT+AGG 0.422202 5_2:+7241015 Msa0753610:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 5_2:+7241588 Msa0753610:three_prime_UTR
TCCAACCAAACCGGTTCAAC+CGG 0.434266 5_2:+7238820 Msa0753610:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 5_2:-7238830 None:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 5_2:-7241043 None:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 5_2:+7241036 Msa0753610:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 5_2:+7239072 Msa0753610:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 5_2:-7238825 None:intergenic
CAGTACTTGGGATTGATGAT+AGG 0.449500 5_2:+7239816 Msa0753610:CDS
AGTCATAGAGAATTTGACTT+TGG 0.459733 5_2:-7241232 None:intergenic
ATATCATCACTGAAGAATCT+AGG 0.462039 5_2:+7239852 Msa0753610:CDS
TGCAGGTGATTTCACGGTTG+AGG 0.465442 5_2:+7241194 Msa0753610:CDS
TTCCAACTTTGGACTTCAGA+TGG 0.477540 5_2:+7241114 Msa0753610:CDS
TTATCATGCAGGTGATTTCA+CGG 0.479801 5_2:+7241188 Msa0753610:CDS
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 5_2:+7238868 Msa0753610:CDS
TTTGAGCACAGTAGAATGAA+TGG 0.487519 5_2:+7241723 Msa0753610:three_prime_UTR
AGCCACAGGTAAGCTTTGCT+TGG 0.490964 5_2:+7239769 Msa0753610:intron
TTGCATACCTTCAGCATACC+TGG 0.490977 5_2:+7240938 Msa0753610:CDS
AAACATCAGACACATGCATA+TGG 0.495895 5_2:-7241703 None:intergenic
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 5_2:-7240990 None:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 5_2:+7238811 Msa0753610:CDS
TGCAACACTGAACATGTTCA+TGG 0.501094 5_2:+7241069 Msa0753610:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 5_2:+7240956 Msa0753610:CDS
GGTGATTTCACGGTTGAGGA+TGG 0.503816 5_2:+7241198 Msa0753610:CDS
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 5_2:-7238821 None:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 5_2:+7241258 Msa0753610:CDS
TCTAGTATGTATAGAACATA+GGG 0.518228 5_2:+7241393 Msa0753610:three_prime_UTR
CACTGAACATGTTCATGGGT+GGG 0.526344 5_2:+7241074 Msa0753610:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 5_2:-7241152 None:intergenic
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 5_2:-7241116 None:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 5_2:-7240956 None:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 5_2:-7240997 None:intergenic
GTCTAGTATGTATAGAACAT+AGG 0.542161 5_2:+7241392 Msa0753610:three_prime_UTR
TTGACTTCCCATTTCCAGCC+GGG 0.542798 5_2:+7240983 Msa0753610:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 5_2:+7241115 Msa0753610:CDS
GCATACCTGGTGGTTTGAAG+TGG 0.546087 5_2:+7240951 Msa0753610:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 5_2:-7238985 None:intergenic
GTGATTTCACGGTTGAGGAT+GGG 0.546700 5_2:+7241199 Msa0753610:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 5_2:+7241016 Msa0753610:CDS
TTGAGCACAGTAGAATGAAT+GGG 0.555488 5_2:+7241724 Msa0753610:three_prime_UTR
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 5_2:+7241571 Msa0753610:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 5_2:+7240955 Msa0753610:CDS
ATGAATGGGAATAAGAGTGT+TGG 0.570486 5_2:+7241738 Msa0753610:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 5_2:-7241001 None:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 5_2:-7241105 None:intergenic
TGCGGAAGATTTGAATGTAT+GGG 0.582252 5_2:+7241589 Msa0753610:three_prime_UTR
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 5_2:+7240957 Msa0753610:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 5_2:+7240982 Msa0753610:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 5_2:+7241406 Msa0753610:three_prime_UTR
ACAAAGGACTGTCAGTACTT+GGG 0.600779 5_2:+7239804 Msa0753610:CDS
AGTTCCTTTCAGAAAACACT+GGG 0.601469 5_2:-7241151 None:intergenic
GATTGCACGCACACTAAAGG+TGG 0.628473 5_2:+7241261 Msa0753610:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 5_2:+7241070 Msa0753610:CDS
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 5_2:+7241031 Msa0753610:CDS
TACTATTGATGATGGCACCA+AGG 0.640156 5_2:+7239080 Msa0753610:CDS
GCAAGTTGACAGATCTGAGG+AGG 0.643594 5_2:-7238901 None:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 5_2:+7241073 Msa0753610:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 5_2:+7240941 Msa0753610:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 5_2:-7240945 None:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 5_2:-7241148 None:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 5_2:-7238904 None:intergenic
TTGGCTATATCAGCTAACAA+AGG 0.690166 5_2:+7239788 Msa0753610:CDS
AGTATGTATAGAACATAGGG+CGG 0.724118 5_2:+7241396 Msa0753610:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG + chr5_2:7239267-7239286 Msa0753610:intron 10.0%
!!! TGGATTGTTTTAAATTTTTT+TGG + chr5_2:7239965-7239984 Msa0753610:intron 15.0%
!!! TGTTATGATATCATATTTTT+GGG + chr5_2:7240060-7240079 Msa0753610:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG + chr5_2:7240271-7240290 Msa0753610:intron 15.0%
!! ACATTGTAATCTATTTAATA+GGG + chr5_2:7240479-7240498 Msa0753610:intron 15.0%
!! CTAAAATAGATTCATAATAA+TGG - chr5_2:7240872-7240891 None:intergenic 15.0%
!!! TTATTATGAATCTATTTTAG+TGG + chr5_2:7240872-7240891 Msa0753610:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG + chr5_2:7241512-7241531 Msa0753610:three_prime_UTR 15.0%
!!! TATACCTTTAGAATCAATTT+TGG + chr5_2:7239397-7239416 Msa0753610:intron 20.0%
!!! GTGTTATGATATCATATTTT+TGG + chr5_2:7240059-7240078 Msa0753610:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG + chr5_2:7240150-7240169 Msa0753610:intron 20.0%
!!! ATAGTTTAAGTTTCTAGATT+TGG + chr5_2:7240243-7240262 Msa0753610:intron 20.0%
!!! CGAGGTTTTTTTTTTAATTT+AGG - chr5_2:7240441-7240460 None:intergenic 20.0%
!!! GATATTTATTGTTTTTATCG+AGG - chr5_2:7240459-7240478 None:intergenic 20.0%
!! CACATTGTAATCTATTTAAT+AGG + chr5_2:7240478-7240497 Msa0753610:intron 20.0%
!! TAGATACTTAACCATAAAAA+AGG - chr5_2:7240529-7240548 None:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG + chr5_2:7240719-7240738 Msa0753610:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG + chr5_2:7241440-7241459 Msa0753610:three_prime_UTR 20.0%
!! TGGTAAATCATGAAAGATTT+TGG - chr5_2:7239013-7239032 None:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG + chr5_2:7239020-7239039 Msa0753610:CDS 25.0%
! CATGATTTACCAATCAATTT+GGG + chr5_2:7239021-7239040 Msa0753610:CDS 25.0%
! AAATGTATGTGTGCTTATTT+CGG + chr5_2:7239217-7239236 Msa0753610:intron 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG + chr5_2:7239323-7239342 Msa0753610:intron 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG + chr5_2:7239492-7239511 Msa0753610:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG + chr5_2:7239493-7239512 Msa0753610:intron 25.0%
!! ATGCAATTGTATTTTCTAGA+TGG + chr5_2:7239945-7239964 Msa0753610:intron 25.0%
! TTCAATGACAACATAAAGAA+AGG - chr5_2:7240008-7240027 None:intergenic 25.0%
!!! ATTGAATAGTTTGATGATGA+GGG + chr5_2:7240022-7240041 Msa0753610:intron 25.0%
! AAACTTAAACTATGTTTGTG+GGG - chr5_2:7240236-7240255 None:intergenic 25.0%
! GAAACTTAAACTATGTTTGT+GGG - chr5_2:7240237-7240256 None:intergenic 25.0%
! AGAAACTTAAACTATGTTTG+TGG - chr5_2:7240238-7240257 None:intergenic 25.0%
! GCAGAAAATAAATGACTATT+TGG - chr5_2:7240505-7240524 None:intergenic 25.0%
!!! TATTTTCTGCACCTTTTTTA+TGG + chr5_2:7240515-7240534 Msa0753610:intron 25.0%
! ATCATCAACTACGAAAATTA+AGG - chr5_2:7240813-7240832 None:intergenic 25.0%
! TTATTCTTCAGAATACATCT+AGG + chr5_2:7241015-7241034 Msa0753610:CDS 25.0%
! TATTCTTCAGAATACATCTA+GGG + chr5_2:7241016-7241035 Msa0753610:CDS 25.0%
! TCTAGTATGTATAGAACATA+GGG + chr5_2:7241393-7241412 Msa0753610:three_prime_UTR 25.0%
!! TGTTCTATCAAGTTTTTGTA+TGG + chr5_2:7241537-7241556 Msa0753610:three_prime_UTR 25.0%
! TAAACTTCAGATATAGTCTT+AGG - chr5_2:7241635-7241654 None:intergenic 25.0%
TAATTCACCTAAAGTTGAAC+CGG - chr5_2:7238842-7238861 None:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG - chr5_2:7239033-7239052 None:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG + chr5_2:7239072-7239091 Msa0753610:CDS 30.0%
AATCAATAAAGCAATCACCT+TGG - chr5_2:7239100-7239119 None:intergenic 30.0%
TCCATTTCGCGAAAAATTTA+GGG - chr5_2:7239153-7239172 None:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG - chr5_2:7239154-7239173 None:intergenic 30.0%
! TGTATGTATGATTCCACTTT+TGG - chr5_2:7239428-7239447 None:intergenic 30.0%
TGGAATCATACATACACTTA+AGG + chr5_2:7239432-7239451 Msa0753610:intron 30.0%
ATATCATCACTGAAGAATCT+AGG + chr5_2:7239852-7239871 Msa0753610:CDS 30.0%
!! CATTGAATAGTTTGATGATG+AGG + chr5_2:7240021-7240040 Msa0753610:intron 30.0%
!! TTGAATAGTTTGATGATGAG+GGG + chr5_2:7240023-7240042 Msa0753610:intron 30.0%
ACCTATAACCACATGTTTAA+GGG - chr5_2:7240364-7240383 None:intergenic 30.0%
AACCTATAACCACATGTTTA+AGG - chr5_2:7240365-7240384 None:intergenic 30.0%
GATAGGACATTGTTTGAAAA+CGG + chr5_2:7240609-7240628 Msa0753610:intron 30.0%
GTTTGAAAACGGAATAACTT+AGG + chr5_2:7240620-7240639 Msa0753610:intron 30.0%
! TTGTTTGAGTTAAACCACAT+TGG + chr5_2:7240770-7240789 Msa0753610:intron 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG + chr5_2:7240880-7240899 Msa0753610:intron 30.0%
! AATTGGACTTTTTATCATGC+AGG + chr5_2:7241177-7241196 Msa0753610:CDS 30.0%
! AGTCATAGAGAATTTGACTT+TGG - chr5_2:7241235-7241254 None:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG - chr5_2:7241360-7241379 None:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG + chr5_2:7241392-7241411 Msa0753610:three_prime_UTR 30.0%
!!! ATTAGAGTTTTGTTAGAGCT+AGG + chr5_2:7241463-7241482 Msa0753610:three_prime_UTR 30.0%
!! TTCTTCTGCTGATTTCGTTT+GGG + chr5_2:7238951-7238970 Msa0753610:CDS 35.0%
! AGGAGAAGATGGTAATTTGT+CGG - chr5_2:7238988-7239007 None:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG - chr5_2:7239008-7239027 None:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG + chr5_2:7239149-7239168 Msa0753610:intron 35.0%
ATTTCGGTTTAGCGTATGTT+TGG + chr5_2:7239233-7239252 Msa0753610:intron 35.0%
! GGAGCCAAAATTGATTCTAA+AGG - chr5_2:7239404-7239423 None:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG - chr5_2:7239425-7239444 None:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG + chr5_2:7239436-7239455 Msa0753610:intron 35.0%
! TTTGTTGTGTGCAGAAAGTT+TGG + chr5_2:7239738-7239757 Msa0753610:intron 35.0%
TTGGCTATATCAGCTAACAA+AGG + chr5_2:7239788-7239807 Msa0753610:CDS 35.0%
! GGATTGATGATAGGAGATAT+TGG + chr5_2:7239825-7239844 Msa0753610:CDS 35.0%
ACAGAATTGATCACAATGCA+GGG - chr5_2:7239918-7239937 None:intergenic 35.0%
AACAGAATTGATCACAATGC+AGG - chr5_2:7239919-7239938 None:intergenic 35.0%
GACTGGAATACATACATACT+TGG + chr5_2:7240167-7240186 Msa0753610:intron 35.0%
ATCAAATGCCCAATATCAAC+AGG + chr5_2:7240312-7240331 Msa0753610:intron 35.0%
TCCCTTAAACATGTGGTTAT+AGG + chr5_2:7240360-7240379 Msa0753610:intron 35.0%
TATAGGTTCAATTCATACCC+AGG + chr5_2:7240377-7240396 Msa0753610:intron 35.0%
ATAGGTTCAATTCATACCCA+GGG + chr5_2:7240378-7240397 Msa0753610:intron 35.0%
GTGAAAACTGGTAAAGTGAT+AGG + chr5_2:7240592-7240611 Msa0753610:intron 35.0%
AGCAAGCATATTTACCAATG+TGG - chr5_2:7240787-7240806 None:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG - chr5_2:7241154-7241173 None:intergenic 35.0%
TTATCATGCAGGTGATTTCA+CGG + chr5_2:7241188-7241207 Msa0753610:CDS 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG - chr5_2:7241314-7241333 None:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG - chr5_2:7241315-7241334 None:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG - chr5_2:7241355-7241374 None:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG + chr5_2:7241396-7241415 Msa0753610:three_prime_UTR 35.0%
TTGAGAAAGTTCTATGCTTG+CGG + chr5_2:7241571-7241590 Msa0753610:three_prime_UTR 35.0%
TTGCGGAAGATTTGAATGTA+TGG + chr5_2:7241588-7241607 Msa0753610:three_prime_UTR 35.0%
TGCGGAAGATTTGAATGTAT+GGG + chr5_2:7241589-7241608 Msa0753610:three_prime_UTR 35.0%
AAACATCAGACACATGCATA+TGG - chr5_2:7241706-7241725 None:intergenic 35.0%
TTTGAGCACAGTAGAATGAA+TGG + chr5_2:7241723-7241742 Msa0753610:three_prime_UTR 35.0%
TTGAGCACAGTAGAATGAAT+GGG + chr5_2:7241724-7241743 Msa0753610:three_prime_UTR 35.0%
! ATGAATGGGAATAAGAGTGT+TGG + chr5_2:7241738-7241757 Msa0753610:three_prime_UTR 35.0%
TCATTTCGATCCAACCAAAC+CGG + chr5_2:7238811-7238830 Msa0753610:CDS 40.0%
TAAAGTTGAACCGGTTGAAC+CGG - chr5_2:7238833-7238852 None:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG + chr5_2:7238950-7238969 Msa0753610:CDS 40.0%
!! TACTATTGATGATGGCACCA+AGG + chr5_2:7239080-7239099 Msa0753610:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr5_2:7239412-7239431 Msa0753610:intron 40.0%
! ACATACACTTAAGGCTGGTT+CGG + chr5_2:7239441-7239460 Msa0753610:intron 40.0%
GATAAGCTGTTTGTCCAAAC+AGG + chr5_2:7239681-7239700 Msa0753610:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG + chr5_2:7239689-7239708 Msa0753610:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG + chr5_2:7239697-7239716 Msa0753610:intron 40.0%
AACAAAGGACTGTCAGTACT+TGG + chr5_2:7239803-7239822 Msa0753610:CDS 40.0%
ACAAAGGACTGTCAGTACTT+GGG + chr5_2:7239804-7239823 Msa0753610:CDS 40.0%
! CAGTACTTGGGATTGATGAT+AGG + chr5_2:7239816-7239835 Msa0753610:CDS 40.0%
!! ATAGTTTGATGATGAGGGGA+AGG + chr5_2:7240027-7240046 Msa0753610:intron 40.0%
GACTGACACCTGTTGATATT+GGG - chr5_2:7240323-7240342 None:intergenic 40.0%
AACCACATGTTTAAGGGACA+CGG - chr5_2:7240358-7240377 None:intergenic 40.0%
! TTCTTCCATCGACTAGTCAA+GGG - chr5_2:7240412-7240431 None:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG - chr5_2:7240413-7240432 None:intergenic 40.0%
AGTGCTCAAGAATAGGACAA+TGG - chr5_2:7240567-7240586 None:intergenic 40.0%
TCCTATTCTTGAGCACTTGA+TGG + chr5_2:7240570-7240589 Msa0753610:intron 40.0%
ACCATCAAGTGCTCAAGAAT+AGG - chr5_2:7240574-7240593 None:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG + chr5_2:7240733-7240752 Msa0753610:intron 40.0%
GAAGAATAAGCTGTATGTCC+CGG - chr5_2:7241004-7241023 None:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG + chr5_2:7241069-7241088 Msa0753610:CDS 40.0%
GCAACACTGAACATGTTCAT+GGG + chr5_2:7241070-7241089 Msa0753610:CDS 40.0%
TTCCAACTTTGGACTTCAGA+TGG + chr5_2:7241114-7241133 Msa0753610:CDS 40.0%
TCCAACTTTGGACTTCAGAT+GGG + chr5_2:7241115-7241134 Msa0753610:CDS 40.0%
CAGTTCCTTTCAGAAAACAC+TGG - chr5_2:7241155-7241174 None:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG + chr5_2:7241153-7241172 Msa0753610:CDS 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG + chr5_2:7241160-7241179 Msa0753610:CDS 40.0%
ATTGATTGCACGCACACTAA+AGG + chr5_2:7241258-7241277 Msa0753610:CDS 40.0%
AAACTGCTCCTAACCATTTC+AGG + chr5_2:7241344-7241363 Msa0753610:exon 40.0%
!!! AGAGTTTTGTTAGAGCTAGG+AGG + chr5_2:7241466-7241485 Msa0753610:three_prime_UTR 40.0%
!!! GAGTTTTGTTAGAGCTAGGA+GGG + chr5_2:7241467-7241486 Msa0753610:three_prime_UTR 40.0%
! GGTTCAACCGGTTCAACTTT+AGG + chr5_2:7238832-7238851 Msa0753610:intron 45.0%
ATTGAAGCGACGCAACTTTC+AGG - chr5_2:7238865-7238884 None:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG + chr5_2:7238868-7238887 Msa0753610:CDS 45.0%
! CGCTTCAATCATCGGTTACA+TGG + chr5_2:7238876-7238895 Msa0753610:CDS 45.0%
! GTTGCAAGTTGACAGATCTG+AGG - chr5_2:7238907-7238926 None:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG - chr5_2:7238999-7239018 None:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG - chr5_2:7239698-7239717 None:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG + chr5_2:7239696-7239715 Msa0753610:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG + chr5_2:7239710-7239729 Msa0753610:intron 45.0%
GTTTGGATTGATAGAGCCAC+AGG + chr5_2:7239755-7239774 Msa0753610:intron 45.0%
GATGAGGGGAAGGTAATTGA+TGG + chr5_2:7240037-7240056 Msa0753610:intron 45.0%
GGACTGACACCTGTTGATAT+TGG - chr5_2:7240324-7240343 None:intergenic 45.0%
AACAGGTGTCAGTCCAAGTA+TGG + chr5_2:7240329-7240348 Msa0753610:intron 45.0%
ACAGGTGTCAGTCCAAGTAT+GGG + chr5_2:7240330-7240349 Msa0753610:intron 45.0%
ACCACATGTTTAAGGGACAC+GGG - chr5_2:7240357-7240376 None:intergenic 45.0%
!! GAGCACTTGATGGTGAAAAC+TGG + chr5_2:7240580-7240599 Msa0753610:intron 45.0%
ATTACGCTTCTCTGCTACTG+AGG + chr5_2:7240675-7240694 Msa0753610:intron 45.0%
TTGCATACCTTCAGCATACC+TGG + chr5_2:7240938-7240957 Msa0753610:CDS 45.0%
ACACTGAACATGTTCATGGG+TGG + chr5_2:7241073-7241092 Msa0753610:CDS 45.0%
CACTGAACATGTTCATGGGT+GGG + chr5_2:7241074-7241093 Msa0753610:CDS 45.0%
TCCTTTCAGAAAACACTGGG+AGG - chr5_2:7241151-7241170 None:intergenic 45.0%
GTGATTTCACGGTTGAGGAT+GGG + chr5_2:7241199-7241218 Msa0753610:CDS 45.0%
CACTAAAGGTGGTCTGTGTT+TGG + chr5_2:7241272-7241291 Msa0753610:CDS 45.0%
!! AATATATAACTTTATAAAAT+TGG + chr5_2:7239600-7239619 Msa0753610:intron 5.0%
! TCCAACCAAACCGGTTCAAC+CGG + chr5_2:7238820-7238839 Msa0753610:CDS 50.0%
TTGAACCGGTTGAACCGGTT+TGG - chr5_2:7238828-7238847 None:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG - chr5_2:7238904-7238923 None:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG + chr5_2:7239446-7239465 Msa0753610:intron 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG + chr5_2:7239702-7239721 Msa0753610:intron 50.0%
AGCCACAGGTAAGCTTTGCT+TGG + chr5_2:7239769-7239788 Msa0753610:intron 50.0%
AGCCAAGCAAAGCTTACCTG+TGG - chr5_2:7239774-7239793 None:intergenic 50.0%
CCACATGTTTAAGGGACACG+GGG - chr5_2:7240356-7240375 None:intergenic 50.0%
GTCAAGGGAGCAATTACCCT+GGG - chr5_2:7240397-7240416 None:intergenic 50.0%
AGTCAAGGGAGCAATTACCC+TGG - chr5_2:7240398-7240417 None:intergenic 50.0%
! TTGCTCCCTTGACTAGTCGA+TGG + chr5_2:7240404-7240423 Msa0753610:intron 50.0%
CATACCTTCAGCATACCTGG+TGG + chr5_2:7240941-7240960 Msa0753610:CDS 50.0%
CAAACCACCAGGTATGCTGA+AGG - chr5_2:7240948-7240967 None:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG + chr5_2:7240951-7240970 Msa0753610:CDS 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG + chr5_2:7240955-7240974 Msa0753610:CDS 50.0%
! GTTGACTTCCCATTTCCAGC+CGG + chr5_2:7240982-7241001 Msa0753610:CDS 50.0%
TTGACTTCCCATTTCCAGCC+GGG + chr5_2:7240983-7241002 Msa0753610:CDS 50.0%
TGTATGTCCCGGCTGGAAAT+GGG - chr5_2:7240993-7241012 None:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG - chr5_2:7241000-7241019 None:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG + chr5_2:7241031-7241050 Msa0753610:CDS 50.0%
ACGACGTCCAAACCTCTTGT+AGG - chr5_2:7241046-7241065 None:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG + chr5_2:7241103-7241122 Msa0753610:CDS 50.0%
GTCCAAAGTTGGAATCGCAC+AGG - chr5_2:7241108-7241127 None:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG - chr5_2:7241119-7241138 None:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG + chr5_2:7241147-7241166 Msa0753610:CDS 50.0%
TGCAGGTGATTTCACGGTTG+AGG + chr5_2:7241194-7241213 Msa0753610:CDS 50.0%
GGTGATTTCACGGTTGAGGA+TGG + chr5_2:7241198-7241217 Msa0753610:CDS 50.0%
GATTGCACGCACACTAAAGG+TGG + chr5_2:7241261-7241280 Msa0753610:CDS 50.0%
!! ACCGGTTGAACCGGTTTGGT+TGG - chr5_2:7238824-7238843 None:intergenic 55.0%
CCCCGTGTCCCTTAAACATG+TGG + chr5_2:7240353-7240372 Msa0753610:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG + chr5_2:7240680-7240699 Msa0753610:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG + chr5_2:7240956-7240975 Msa0753610:CDS 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG + chr5_2:7240957-7240976 Msa0753610:CDS 55.0%
CTGTATGTCCCGGCTGGAAA+TGG - chr5_2:7240994-7241013 None:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG + chr5_2:7241036-7241055 Msa0753610:CDS 55.0%
!! GAACATAGGGCGGTGTCATG+AGG + chr5_2:7241406-7241425 Msa0753610:three_prime_UTR 55.0%
CCCGTCCACTTCAAACCACC+AGG - chr5_2:7240959-7240978 None:intergenic 60.0%
AGGGACACGGGGCCCATACT+TGG - chr5_2:7240345-7240364 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7238793 7241772 7238793 ID=Msa0753610;Name=Msa0753610
chr5_2 mRNA 7238793 7241772 7238793 ID=Msa0753610-mRNA-1;Parent=Msa0753610;Name=Msa0753610-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.33|0.25|1|0.33|0.25|4|416|272
chr5_2 exon 7238793 7238841 7238793 ID=Msa0753610-mRNA-1:exon:3211;Parent=Msa0753610-mRNA-1
chr5_2 exon 7238854 7239101 7238854 ID=Msa0753610-mRNA-1:exon:3212;Parent=Msa0753610-mRNA-1
chr5_2 exon 7239782 7239873 7239782 ID=Msa0753610-mRNA-1:exon:3213;Parent=Msa0753610-mRNA-1
chr5_2 exon 7240927 7241772 7240927 ID=Msa0753610-mRNA-1:exon:3214;Parent=Msa0753610-mRNA-1
chr5_2 CDS 7238793 7238841 7238793 ID=Msa0753610-mRNA-1:cds;Parent=Msa0753610-mRNA-1
chr5_2 CDS 7238854 7239101 7238854 ID=Msa0753610-mRNA-1:cds;Parent=Msa0753610-mRNA-1
chr5_2 CDS 7239782 7239873 7239782 ID=Msa0753610-mRNA-1:cds;Parent=Msa0753610-mRNA-1
chr5_2 CDS 7240927 7241356 7240927 ID=Msa0753610-mRNA-1:cds;Parent=Msa0753610-mRNA-1
chr5_2 three_prime_UTR 7241357 7241772 7241357 ID=Msa0753610-mRNA-1:three_prime_utr;Parent=Msa0753610-mRNA-1
Gene Sequence

>Msa0753610

ATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGCTTTGCTTGGCTATATCAGCTAACAAAGGACTGTCAGTACTTGGGATTGATGATAGGAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTATCATGCAGGTGATTTCACGGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa0753610

MGIRFSSFRSNQTGSTGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*