AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0753510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753510 MtrunA17_Chr5g0397981 95.667 300 7 2 12 308 5 301 0.0 592
Msa0753510 MtrunA17_Chr4g0057281 76.208 269 61 2 42 308 19 286 3.00e-158 443
Msa0753510 MtrunA17_Chr8g0350401 56.401 289 117 3 23 303 1 288 2.22e-119 344
Msa0753510 MtrunA17_Chr7g0265551 56.314 293 112 5 23 302 73 362 2.19e-112 330
Msa0753510 MtrunA17_Chr1g0203751 54.448 281 126 2 21 300 7 286 4.36e-112 326
Msa0753510 MtrunA17_Chr6g0472801 55.769 260 113 2 40 298 20 278 1.89e-110 322
Msa0753510 MtrunA17_Chr7g0272101 24.453 274 173 8 45 300 4 261 1.42e-27 108
Msa0753510 MtrunA17_Chr4g0010881 25.379 264 161 8 45 299 4 240 5.30e-24 97.8
Msa0753510 MtrunA17_Chr1g0189671 34.722 144 91 3 44 186 6 147 7.29e-22 92.8
Msa0753510 MtrunA17_Chr1g0189611 34.932 146 91 3 44 186 6 150 2.46e-21 91.3
Msa0753510 MtrunA17_Chr1g0189591 33.117 154 96 4 35 183 6 157 7.98e-21 90.5
Msa0753510 MtrunA17_Chr1g0189641 33.562 146 90 4 44 186 6 147 5.01e-19 85.1
Msa0753510 MtrunA17_Chr1g0189751 32.667 150 94 4 40 186 2 147 1.44e-18 84.3
Msa0753510 MtrunA17_Chr1g0189721 25.940 266 184 6 40 295 2 264 2.52e-18 82.8
Msa0753510 MtrunA17_Chr7g0272091 22.648 287 182 9 40 295 5 282 3.17e-18 83.2
Msa0753510 MtrunA17_Chr1g0189621 26.236 263 178 9 44 295 6 263 4.17e-18 82.4
Msa0753510 MtrunA17_Chr1g0189821 25.725 276 169 10 44 297 6 267 2.47e-17 80.1
Msa0753510 MtrunA17_Chr1g0203281 31.250 144 96 3 44 186 6 147 2.69e-17 80.1
Msa0753510 MtrunA17_Chr1g0190071 31.973 147 95 3 44 186 6 151 5.25e-17 79.7
Msa0753510 MtrunA17_Chr1g0192371 28.049 164 112 4 40 198 6 168 7.60e-16 76.3
Msa0753510 MtrunA17_Chr1g0189781 29.870 154 97 4 40 186 6 155 1.49e-15 75.5
Msa0753510 MtrunA17_Chr1g0189771 28.571 161 102 4 34 186 4 159 4.97e-15 72.0
Msa0753510 MtrunA17_Chr3g0097191 31.944 144 91 4 42 183 10 148 1.87e-14 72.0
Msa0753510 MtrunA17_Chr1g0203331 31.008 129 86 3 59 186 6 132 5.75e-14 70.5
Msa0753510 MtrunA17_Chr1g0189661 30.872 149 93 5 44 186 6 150 2.23e-13 69.3
Msa0753510 MtrunA17_Chr1g0189711 28.431 204 120 7 44 238 6 192 1.22e-12 67.0
Msa0753510 MtrunA17_Chr3g0095261 31.852 135 83 5 42 174 10 137 7.70e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753510 AT1G12710.1 67.181 259 83 2 42 299 28 285 3.09e-128 367
Msa0753510 AT1G12710.2 57.807 301 83 3 42 299 28 327 5.49e-119 345
Msa0753510 AT3G61060.1 58.242 273 111 3 31 301 14 285 5.16e-118 341
Msa0753510 AT3G61060.2 58.029 274 111 4 31 301 14 286 2.07e-116 337
Msa0753510 AT1G63090.1 59.507 284 107 3 23 299 1 283 2.14e-114 332
Msa0753510 AT3G53000.1 53.333 285 120 3 23 295 1 284 1.16e-103 305
Msa0753510 AT5G52120.1 48.772 285 137 3 23 299 1 284 5.61e-98 290
Msa0753510 AT2G02360.1 34.591 159 94 4 31 186 3 154 1.70e-26 105
Msa0753510 AT2G02230.1 32.759 174 105 7 39 206 29 196 2.83e-23 98.2
Msa0753510 AT2G02240.1 34.752 141 86 3 45 184 64 199 2.59e-22 95.5
Msa0753510 AT1G09155.1 24.823 282 184 7 45 299 3 283 8.21e-22 93.6
Msa0753510 AT2G02340.1 32.927 164 100 5 40 198 34 192 8.50e-22 93.6
Msa0753510 AT2G02350.2 36.054 147 83 5 38 180 20 159 1.69e-21 92.8
Msa0753510 AT2G02250.1 30.667 150 95 3 37 183 42 185 2.56e-21 92.4
Msa0753510 AT2G02320.1 31.285 179 110 6 25 198 22 192 7.29e-20 88.2
Msa0753510 AT5G24560.1 26.347 167 112 4 44 209 5 161 1.94e-18 82.0
Msa0753510 AT2G02310.1 31.973 147 90 4 40 183 43 182 2.23e-18 84.3
Msa0753510 AT1G56250.1 24.232 293 164 8 45 295 4 280 1.50e-16 78.6
Msa0753510 AT1G56240.1 24.028 283 185 5 45 299 4 284 5.71e-16 77.0
Msa0753510 AT1G80110.1 29.054 148 97 4 42 187 1 142 8.06e-16 76.3
Msa0753510 AT2G02300.1 26.891 238 141 10 38 264 30 245 1.07e-12 67.8

Find 98 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 5_2:+7153299 Msa0753510:CDS
CATTCAATCTCAATCTTCTA+TGG 0.161577 5_2:+7153053 Msa0753510:CDS
AATTTCTTCTGTTAATTAAA+TGG 0.179440 5_2:+7155772 Msa0753510:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 5_2:-7153289 None:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 5_2:+7155604 Msa0753510:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 5_2:-7153141 None:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 5_2:+7153229 Msa0753510:CDS
GGGTGCAGAAAGATTGAAAA+AGG 0.264780 5_2:-7153014 None:intergenic
GGTTCAACCGGTTCAACTTT+AGG 0.271212 5_2:+7153111 Msa0753510:CDS
TAAACTTCAGATATACTCTT+AGG 0.292898 5_2:-7155892 None:intergenic
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 5_2:-7155251 None:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 5_2:+7153230 Msa0753510:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 5_2:+7155532 Msa0753510:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 5_2:+7153300 Msa0753510:CDS
TTGAGATTGAATGGTAATAA+TGG 0.355846 5_2:-7153043 None:intergenic
AAACATCAGACACATGTATA+TGG 0.363548 5_2:-7155963 None:intergenic
AGCCTGTGCGATTCCAACTT+TGG 0.369551 5_2:+7155363 Msa0753510:CDS
CGCTTCAATCATCGGTTACA+TGG 0.370713 5_2:+7153155 Msa0753510:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 5_2:+7155420 Msa0753510:CDS
TCTTTCTACCTGAAATGGTT+AGG 0.406400 5_2:-7155612 None:intergenic
TGTCCTTGGTGGACAAGGTT+TGG 0.418293 5_2:-7152936 None:intergenic
TTATTCTTCAGAATACATCT+AGG 0.422202 5_2:+7155275 Msa0753510:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 5_2:+7155848 Msa0753510:three_prime_UTR
TCCAACCAAACCGGTTCAAC+CGG 0.434266 5_2:+7153099 Msa0753510:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 5_2:-7153109 None:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 5_2:-7155303 None:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 5_2:+7155296 Msa0753510:CDS
CATAGAAGATTGAGATTGAA+TGG 0.442851 5_2:-7153052 None:intergenic
GAATTGATGATAGAAGATAT+TGG 0.446914 5_2:+7154103 Msa0753510:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 5_2:+7153351 Msa0753510:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 5_2:-7153104 None:intergenic
AGTCATAGAGAATTTGACTT+TGG 0.459733 5_2:-7155492 None:intergenic
CCTTGTCCACCAAGGACAAC+CGG 0.461370 5_2:+7152941 Msa0753510:five_prime_UTR
ATATCATCACTGAAGAATCT+AGG 0.462039 5_2:+7154130 Msa0753510:CDS
AGCCACGGGCAAGCTTTGCT+TGG 0.464990 5_2:+7154047 Msa0753510:CDS
TGCAGGTGATTTCACGGTTG+AGG 0.465442 5_2:+7155454 Msa0753510:CDS
TTCCAACTTTGGACTTCAGA+TGG 0.477540 5_2:+7155374 Msa0753510:CDS
TTATCATGCAGGTGATTTCA+CGG 0.479801 5_2:+7155448 Msa0753510:CDS
TAATTCACCTAAAGTTGAAC+CGG 0.483165 5_2:-7153118 None:intergenic
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 5_2:+7153147 Msa0753510:CDS
ACGCGTACACCGGTTGTCCT+TGG 0.487127 5_2:-7152950 None:intergenic
TTTGAGCACAGTAGAATGAA+TGG 0.487519 5_2:+7155983 Msa0753510:three_prime_UTR
TTGCATACCTTCAGCATACC+TGG 0.490977 5_2:+7155198 Msa0753510:CDS
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 5_2:-7155250 None:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 5_2:+7153090 Msa0753510:CDS
CCGGTTGTCCTTGGTGGACA+AGG 0.499332 5_2:-7152941 None:intergenic
TGCAACACTGAACATGTTCA+TGG 0.501094 5_2:+7155329 Msa0753510:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 5_2:+7155216 Msa0753510:CDS
AACAAGGGACTATCAGTACT+TGG 0.501726 5_2:+7154081 Msa0753510:CDS
GGTGATTTCACGGTTGAGGA+TGG 0.503816 5_2:+7155458 Msa0753510:CDS
CTTGGCTATATCAGCTAACA+AGG 0.503821 5_2:+7154065 Msa0753510:CDS
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 5_2:-7153100 None:intergenic
AATGGTAATAATGGAAAAGA+GGG 0.510959 5_2:-7153034 None:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 5_2:+7155518 Msa0753510:CDS
TCTAGTATGTATAGAACATA+GGG 0.518228 5_2:+7155653 Msa0753510:three_prime_UTR
ATTCAATCTCAATCTTCTAT+GGG 0.519119 5_2:+7153054 Msa0753510:CDS
CACTGAACATGTTCATGGGT+GGG 0.526344 5_2:+7155334 Msa0753510:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 5_2:-7155412 None:intergenic
CGTACACCGGTTGTCCTTGG+TGG 0.532429 5_2:-7152947 None:intergenic
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 5_2:-7155376 None:intergenic
GAATGGTAATAATGGAAAAG+AGG 0.536724 5_2:-7153035 None:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 5_2:-7155216 None:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 5_2:-7155257 None:intergenic
TTGACTTCCCATTTCCAGCC+GGG 0.542798 5_2:+7155243 Msa0753510:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 5_2:+7155375 Msa0753510:CDS
GCATACCTGGTGGTTTGAAG+TGG 0.546087 5_2:+7155211 Msa0753510:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 5_2:-7153264 None:intergenic
GTGATTTCACGGTTGAGGAT+GGG 0.546700 5_2:+7155459 Msa0753510:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 5_2:+7155276 Msa0753510:CDS
TTGAGCACAGTAGAATGAAT+GGG 0.555488 5_2:+7155984 Msa0753510:three_prime_UTR
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 5_2:+7155831 Msa0753510:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 5_2:+7155215 Msa0753510:CDS
GTCTAGTATGTATAGAACAT+AGG 0.562493 5_2:+7155652 Msa0753510:three_prime_UTR
GTTTGGATTGATAGAGCCAC+GGG 0.562866 5_2:+7154033 Msa0753510:CDS
ATGAATGGGAATAAGAGTGT+TGG 0.570486 5_2:+7155998 Msa0753510:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 5_2:-7155261 None:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 5_2:-7155365 None:intergenic
TGCGGAAGATTTGAATGTAT+GGG 0.582252 5_2:+7155849 Msa0753510:three_prime_UTR
ATGTACAGCAACGCGTACAC+CGG 0.585734 5_2:-7152960 None:intergenic
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 5_2:+7155217 Msa0753510:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 5_2:+7155242 Msa0753510:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 5_2:+7155666 Msa0753510:three_prime_UTR
AGTTCCTTTCAGAAAACACT+GGG 0.601469 5_2:-7155411 None:intergenic
AGTTTGGATTGATAGAGCCA+CGG 0.603796 5_2:+7154032 Msa0753510:CDS
GATTGCACGCACACTAAAGG+TGG 0.628473 5_2:+7155521 Msa0753510:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 5_2:+7155330 Msa0753510:CDS
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 5_2:+7155291 Msa0753510:CDS
TACTATTGATGATGGCACCA+AGG 0.640156 5_2:+7153359 Msa0753510:CDS
AGCCAAGCAAAGCTTGCCCG+TGG 0.641717 5_2:-7154049 None:intergenic
GCAAGTTGACAGATCTGAGG+AGG 0.643594 5_2:-7153180 None:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 5_2:+7155333 Msa0753510:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 5_2:+7155201 Msa0753510:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 5_2:-7155205 None:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 5_2:-7155408 None:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 5_2:-7153183 None:intergenic
AGTATGTATAGAACATAGGG+CGG 0.724118 5_2:+7155656 Msa0753510:three_prime_UTR
ATACCAAACCTTGTCCACCA+AGG 0.728318 5_2:+7152933 None:intergenic
TTGGCTATATCAGCTAACAA+GGG 0.728498 5_2:+7154066 Msa0753510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG + chr5_2:7153545-7153564 Msa0753510:intron 10.0%
!! ACATTATAATCTATTTAATA+GGG + chr5_2:7154740-7154759 Msa0753510:intron 10.0%
!! CTAAAATAGATTTATAATAA+TGG - chr5_2:7155132-7155151 None:intergenic 10.0%
!!! TTATTATAAATCTATTTTAG+TGG + chr5_2:7155132-7155151 Msa0753510:intron 10.0%
!!! TGGATGTTTTAATTTTTTTT+TGG + chr5_2:7154245-7154264 Msa0753510:intron 15.0%
!!! TGTTATGATATGATATTTTT+GGG + chr5_2:7154340-7154359 Msa0753510:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG + chr5_2:7154534-7154553 Msa0753510:intron 15.0%
!! CACATTATAATCTATTTAAT+AGG + chr5_2:7154739-7154758 Msa0753510:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG + chr5_2:7155772-7155791 Msa0753510:three_prime_UTR 15.0%
!! ATAAGAAAAAAGAGAGAAAT+GGG - chr5_2:7152988-7153007 None:intergenic 20.0%
!!! TATACCTTTAGAATCAATTT+TGG + chr5_2:7153675-7153694 Msa0753510:intron 20.0%
!!! GTGTTATGATATGATATTTT+TGG + chr5_2:7154339-7154358 Msa0753510:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG + chr5_2:7154425-7154444 Msa0753510:intron 20.0%
!!! ATAGTTTAAGTTTCTAGATT+TGG + chr5_2:7154506-7154525 Msa0753510:intron 20.0%
!!! CGAGGTTTTTTTTTTAATTT+AGG - chr5_2:7154702-7154721 None:intergenic 20.0%
!!! GATATTTATTGTTTTTATCG+AGG - chr5_2:7154720-7154739 None:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG + chr5_2:7154979-7154998 Msa0753510:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG + chr5_2:7155700-7155719 Msa0753510:three_prime_UTR 20.0%
! CATAAGAAAAAAGAGAGAAA+TGG - chr5_2:7152989-7153008 None:intergenic 25.0%
!! AATGGTAATAATGGAAAAGA+GGG - chr5_2:7153037-7153056 None:intergenic 25.0%
! TTGAGATTGAATGGTAATAA+TGG - chr5_2:7153046-7153065 None:intergenic 25.0%
! ATTCAATCTCAATCTTCTAT+GGG + chr5_2:7153054-7153073 Msa0753510:CDS 25.0%
!! TGGTAAATCATGAAAGATTT+TGG - chr5_2:7153292-7153311 None:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG + chr5_2:7153299-7153318 Msa0753510:CDS 25.0%
! CATGATTTACCAATCAATTT+GGG + chr5_2:7153300-7153319 Msa0753510:CDS 25.0%
! AATCAATAAAGTAATCACCT+TGG - chr5_2:7153379-7153398 None:intergenic 25.0%
!! AAATGTATGTGTGCTTATTT+TGG + chr5_2:7153495-7153514 Msa0753510:intron 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG + chr5_2:7153601-7153620 Msa0753510:intron 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG + chr5_2:7153770-7153789 Msa0753510:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG + chr5_2:7153771-7153790 Msa0753510:intron 25.0%
!! GAATTGATGATAGAAGATAT+TGG + chr5_2:7154103-7154122 Msa0753510:CDS 25.0%
!! GCAATATTGTATTTTCTAGA+TGG + chr5_2:7154225-7154244 Msa0753510:intron 25.0%
! TTCAATGACAACATAAAGAA+AGG - chr5_2:7154288-7154307 None:intergenic 25.0%
!!! ATTGAATAGTTTGATGATGA+GGG + chr5_2:7154302-7154321 Msa0753510:intron 25.0%
! AAACTTAAACTATGTTTGTG+GGG - chr5_2:7154499-7154518 None:intergenic 25.0%
! GAAACTTAAACTATGTTTGT+GGG - chr5_2:7154500-7154519 None:intergenic 25.0%
! AGAAACTTAAACTATGTTTG+TGG - chr5_2:7154501-7154520 None:intergenic 25.0%
! CAGAAAATCAAATGACTATT+TGG - chr5_2:7154766-7154785 None:intergenic 25.0%
!!! GATTTTCTGCATCTTTTTTA+TGG + chr5_2:7154777-7154796 Msa0753510:intron 25.0%
! TTATTCTTCAGAATACATCT+AGG + chr5_2:7155275-7155294 Msa0753510:CDS 25.0%
! TATTCTTCAGAATACATCTA+GGG + chr5_2:7155276-7155295 Msa0753510:CDS 25.0%
! TCTAGTATGTATAGAACATA+GGG + chr5_2:7155653-7155672 Msa0753510:three_prime_UTR 25.0%
!! TGTTCTATCAAGTTTTTGTA+TGG + chr5_2:7155797-7155816 Msa0753510:three_prime_UTR 25.0%
! TAAACTTCAGATATACTCTT+AGG - chr5_2:7155895-7155914 None:intergenic 25.0%
! GAATGGTAATAATGGAAAAG+AGG - chr5_2:7153038-7153057 None:intergenic 30.0%
CATAGAAGATTGAGATTGAA+TGG - chr5_2:7153055-7153074 None:intergenic 30.0%
CATTCAATCTCAATCTTCTA+TGG + chr5_2:7153053-7153072 Msa0753510:CDS 30.0%
TAATTCACCTAAAGTTGAAC+CGG - chr5_2:7153121-7153140 None:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG - chr5_2:7153312-7153331 None:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG + chr5_2:7153351-7153370 Msa0753510:CDS 30.0%
TCCATTTCGCGAAAAATTTA+GGG - chr5_2:7153432-7153451 None:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG - chr5_2:7153433-7153452 None:intergenic 30.0%
!!! ATTTTGGTTTAGCGTATGTT+TGG + chr5_2:7153511-7153530 Msa0753510:intron 30.0%
! TGTATGTATGATTCCACTTT+TGG - chr5_2:7153706-7153725 None:intergenic 30.0%
TGGAATCATACATACACTTA+AGG + chr5_2:7153710-7153729 Msa0753510:intron 30.0%
!! TTTTTGTTGTGCAGAAAGTT+TGG + chr5_2:7154016-7154035 Msa0753510:intron 30.0%
ATATCATCACTGAAGAATCT+AGG + chr5_2:7154130-7154149 Msa0753510:CDS 30.0%
!! CATTGAATAGTTTGATGATG+AGG + chr5_2:7154301-7154320 Msa0753510:intron 30.0%
!! TTGAATAGTTTGATGATGAG+GGG + chr5_2:7154303-7154322 Msa0753510:intron 30.0%
GACTAACACATGTTGATATT+GGG - chr5_2:7154586-7154605 None:intergenic 30.0%
GATAGGACATTGTTTGAAAA+TGG + chr5_2:7154870-7154889 Msa0753510:intron 30.0%
! TTGTTTGAGTTAAACCACAT+TGG + chr5_2:7155030-7155049 Msa0753510:intron 30.0%
ATCATCAACTGCGAAAATTA+AGG - chr5_2:7155073-7155092 None:intergenic 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG + chr5_2:7155140-7155159 Msa0753510:intron 30.0%
! AATTGGACTTTTTATCATGC+AGG + chr5_2:7155437-7155456 Msa0753510:CDS 30.0%
! AGTCATAGAGAATTTGACTT+TGG - chr5_2:7155495-7155514 None:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG - chr5_2:7155620-7155639 None:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG + chr5_2:7155652-7155671 Msa0753510:three_prime_UTR 30.0%
!!! ATTAGAGTTTTGTTAGAGCT+AGG + chr5_2:7155723-7155742 Msa0753510:three_prime_UTR 30.0%
AAACATCAGACACATGTATA+TGG - chr5_2:7155966-7155985 None:intergenic 30.0%
GGTGCAGAAAGATTGAAAAA+GGG - chr5_2:7153016-7153035 None:intergenic 35.0%
!! TTCTTCTGCTGATTTCGTTT+GGG + chr5_2:7153230-7153249 Msa0753510:CDS 35.0%
! AGGAGAAGATGGTAATTTGT+CGG - chr5_2:7153267-7153286 None:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG - chr5_2:7153287-7153306 None:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG + chr5_2:7153428-7153447 Msa0753510:intron 35.0%
! GGAGCCAAAATTGATTCTAA+AGG - chr5_2:7153682-7153701 None:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG - chr5_2:7153703-7153722 None:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG + chr5_2:7153714-7153733 Msa0753510:intron 35.0%
TTGGCTATATCAGCTAACAA+GGG + chr5_2:7154066-7154085 Msa0753510:CDS 35.0%
AACAGAATTGATCACAATGC+AGG - chr5_2:7154197-7154216 None:intergenic 35.0%
AGACTGGAATACATTCAACT+TGG + chr5_2:7154441-7154460 Msa0753510:intron 35.0%
GGACTAACACATGTTGATAT+TGG - chr5_2:7154587-7154606 None:intergenic 35.0%
AACATGTGTTAGTCCAAGTA+TGG + chr5_2:7154592-7154611 Msa0753510:intron 35.0%
ACATGTGTTAGTCCAAGTAT+GGG + chr5_2:7154593-7154612 Msa0753510:intron 35.0%
ACCTGTAACCACATGTTTAA+GGG - chr5_2:7154627-7154646 None:intergenic 35.0%
AACCTGTAACCACATGTTTA+AGG - chr5_2:7154628-7154647 None:intergenic 35.0%
GGTGAAAATGGTAAAGTGAT+AGG + chr5_2:7154853-7154872 Msa0753510:intron 35.0%
AGCAAGCATATTTACCAATG+TGG - chr5_2:7155047-7155066 None:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG - chr5_2:7155414-7155433 None:intergenic 35.0%
TTATCATGCAGGTGATTTCA+CGG + chr5_2:7155448-7155467 Msa0753510:CDS 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG - chr5_2:7155574-7155593 None:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG - chr5_2:7155575-7155594 None:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG - chr5_2:7155615-7155634 None:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG + chr5_2:7155656-7155675 Msa0753510:three_prime_UTR 35.0%
TTGAGAAAGTTCTATGCTTG+CGG + chr5_2:7155831-7155850 Msa0753510:three_prime_UTR 35.0%
TTGCGGAAGATTTGAATGTA+TGG + chr5_2:7155848-7155867 Msa0753510:three_prime_UTR 35.0%
TGCGGAAGATTTGAATGTAT+GGG + chr5_2:7155849-7155868 Msa0753510:three_prime_UTR 35.0%
TTTGAGCACAGTAGAATGAA+TGG + chr5_2:7155983-7156002 Msa0753510:three_prime_UTR 35.0%
TTGAGCACAGTAGAATGAAT+GGG + chr5_2:7155984-7156003 Msa0753510:three_prime_UTR 35.0%
! ATGAATGGGAATAAGAGTGT+TGG + chr5_2:7155998-7156017 Msa0753510:three_prime_UTR 35.0%
GGGTGCAGAAAGATTGAAAA+AGG - chr5_2:7153017-7153036 None:intergenic 40.0%
TCATTTCGATCCAACCAAAC+CGG + chr5_2:7153090-7153109 Msa0753510:CDS 40.0%
TAAAGTTGAACCGGTTGAAC+CGG - chr5_2:7153112-7153131 None:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG + chr5_2:7153229-7153248 Msa0753510:CDS 40.0%
!! TACTATTGATGATGGCACCA+AGG + chr5_2:7153359-7153378 Msa0753510:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr5_2:7153690-7153709 Msa0753510:intron 40.0%
! ACATACACTTAAGGCTGGTT+CGG + chr5_2:7153719-7153738 Msa0753510:intron 40.0%
GATAAGCTGTTTGTCCAAAC+AGG + chr5_2:7153959-7153978 Msa0753510:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG + chr5_2:7153967-7153986 Msa0753510:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG + chr5_2:7153975-7153994 Msa0753510:intron 40.0%
AGTTTGGATTGATAGAGCCA+CGG + chr5_2:7154032-7154051 Msa0753510:CDS 40.0%
CTTGGCTATATCAGCTAACA+AGG + chr5_2:7154065-7154084 Msa0753510:CDS 40.0%
AACAAGGGACTATCAGTACT+TGG + chr5_2:7154081-7154100 Msa0753510:CDS 40.0%
!! ATAGTTTGATGATGAGGGGA+AGG + chr5_2:7154307-7154326 Msa0753510:intron 40.0%
AACCACATGTTTAAGGGACA+CGG - chr5_2:7154621-7154640 None:intergenic 40.0%
TCCCTTAAACATGTGGTTAC+AGG + chr5_2:7154623-7154642 Msa0753510:intron 40.0%
TACAGGTTCAATTCATACCC+AGG + chr5_2:7154640-7154659 Msa0753510:intron 40.0%
ACAGGTTCAATTCATACCCA+GGG + chr5_2:7154641-7154660 Msa0753510:intron 40.0%
! TTCTTCCATCGACTAGTCAA+GGG - chr5_2:7154675-7154694 None:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG - chr5_2:7154676-7154695 None:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG + chr5_2:7154993-7155012 Msa0753510:intron 40.0%
GAAGAATAAGCTGTATGTCC+CGG - chr5_2:7155264-7155283 None:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG + chr5_2:7155329-7155348 Msa0753510:CDS 40.0%
GCAACACTGAACATGTTCAT+GGG + chr5_2:7155330-7155349 Msa0753510:CDS 40.0%
TTCCAACTTTGGACTTCAGA+TGG + chr5_2:7155374-7155393 Msa0753510:CDS 40.0%
TCCAACTTTGGACTTCAGAT+GGG + chr5_2:7155375-7155394 Msa0753510:CDS 40.0%
CAGTTCCTTTCAGAAAACAC+TGG - chr5_2:7155415-7155434 None:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG + chr5_2:7155413-7155432 Msa0753510:CDS 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG + chr5_2:7155420-7155439 Msa0753510:CDS 40.0%
ATTGATTGCACGCACACTAA+AGG + chr5_2:7155518-7155537 Msa0753510:CDS 40.0%
AAACTGCTCCTAACCATTTC+AGG + chr5_2:7155604-7155623 Msa0753510:exon 40.0%
!!! AGAGTTTTGTTAGAGCTAGG+AGG + chr5_2:7155726-7155745 Msa0753510:three_prime_UTR 40.0%
!!! GAGTTTTGTTAGAGCTAGGA+GGG + chr5_2:7155727-7155746 Msa0753510:three_prime_UTR 40.0%
! GGTTCAACCGGTTCAACTTT+AGG + chr5_2:7153111-7153130 Msa0753510:CDS 45.0%
ATTGAAGCGACGCAACTTTC+AGG - chr5_2:7153144-7153163 None:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG + chr5_2:7153147-7153166 Msa0753510:CDS 45.0%
! CGCTTCAATCATCGGTTACA+TGG + chr5_2:7153155-7153174 Msa0753510:CDS 45.0%
! GTTGCAAGTTGACAGATCTG+AGG - chr5_2:7153186-7153205 None:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG - chr5_2:7153278-7153297 None:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG - chr5_2:7153976-7153995 None:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG + chr5_2:7153974-7153993 Msa0753510:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG + chr5_2:7153988-7154007 Msa0753510:intron 45.0%
GTTTGGATTGATAGAGCCAC+GGG + chr5_2:7154033-7154052 Msa0753510:CDS 45.0%
GATGAGGGGAAGGTAATTGA+TGG + chr5_2:7154317-7154336 Msa0753510:intron 45.0%
ACCACATGTTTAAGGGACAC+GGG - chr5_2:7154620-7154639 None:intergenic 45.0%
GTCAAGGGATCAATTACCCT+GGG - chr5_2:7154660-7154679 None:intergenic 45.0%
AGTCAAGGGATCAATTACCC+TGG - chr5_2:7154661-7154680 None:intergenic 45.0%
! TTGATCCCTTGACTAGTCGA+TGG + chr5_2:7154667-7154686 Msa0753510:intron 45.0%
TCCTATTCTCGAGCACTTGA+TGG + chr5_2:7154832-7154851 Msa0753510:intron 45.0%
ACCATCAAGTGCTCGAGAAT+AGG - chr5_2:7154836-7154855 None:intergenic 45.0%
!! CGAGCACTTGATGGTGAAAA+TGG + chr5_2:7154841-7154860 Msa0753510:intron 45.0%
ATTACGCTTCTCTGCTACTG+AGG + chr5_2:7154935-7154954 Msa0753510:intron 45.0%
TTGCATACCTTCAGCATACC+TGG + chr5_2:7155198-7155217 Msa0753510:CDS 45.0%
ACACTGAACATGTTCATGGG+TGG + chr5_2:7155333-7155352 Msa0753510:CDS 45.0%
CACTGAACATGTTCATGGGT+GGG + chr5_2:7155334-7155353 Msa0753510:CDS 45.0%
TCCTTTCAGAAAACACTGGG+AGG - chr5_2:7155411-7155430 None:intergenic 45.0%
GTGATTTCACGGTTGAGGAT+GGG + chr5_2:7155459-7155478 Msa0753510:CDS 45.0%
CACTAAAGGTGGTCTGTGTT+TGG + chr5_2:7155532-7155551 Msa0753510:CDS 45.0%
!! ATATATAATCTTTATAAAAT+TGG + chr5_2:7153879-7153898 Msa0753510:intron 5.0%
! TGTCCTTGGTGGACAAGGTT+TGG - chr5_2:7152939-7152958 None:intergenic 50.0%
ATGTACAGCAACGCGTACAC+CGG - chr5_2:7152963-7152982 None:intergenic 50.0%
! TCCAACCAAACCGGTTCAAC+CGG + chr5_2:7153099-7153118 Msa0753510:CDS 50.0%
TTGAACCGGTTGAACCGGTT+TGG - chr5_2:7153107-7153126 None:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG - chr5_2:7153183-7153202 None:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG + chr5_2:7153724-7153743 Msa0753510:intron 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG + chr5_2:7153980-7153999 Msa0753510:intron 50.0%
CCACATGTTTAAGGGACACG+GGG - chr5_2:7154619-7154638 None:intergenic 50.0%
CATACCTTCAGCATACCTGG+TGG + chr5_2:7155201-7155220 Msa0753510:CDS 50.0%
CAAACCACCAGGTATGCTGA+AGG - chr5_2:7155208-7155227 None:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG + chr5_2:7155211-7155230 Msa0753510:CDS 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG + chr5_2:7155215-7155234 Msa0753510:CDS 50.0%
! GTTGACTTCCCATTTCCAGC+CGG + chr5_2:7155242-7155261 Msa0753510:CDS 50.0%
TTGACTTCCCATTTCCAGCC+GGG + chr5_2:7155243-7155262 Msa0753510:CDS 50.0%
TGTATGTCCCGGCTGGAAAT+GGG - chr5_2:7155253-7155272 None:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG - chr5_2:7155260-7155279 None:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG + chr5_2:7155291-7155310 Msa0753510:CDS 50.0%
ACGACGTCCAAACCTCTTGT+AGG - chr5_2:7155306-7155325 None:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG + chr5_2:7155363-7155382 Msa0753510:CDS 50.0%
GTCCAAAGTTGGAATCGCAC+AGG - chr5_2:7155368-7155387 None:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG - chr5_2:7155379-7155398 None:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG + chr5_2:7155407-7155426 Msa0753510:CDS 50.0%
TGCAGGTGATTTCACGGTTG+AGG + chr5_2:7155454-7155473 Msa0753510:CDS 50.0%
GGTGATTTCACGGTTGAGGA+TGG + chr5_2:7155458-7155477 Msa0753510:CDS 50.0%
GATTGCACGCACACTAAAGG+TGG + chr5_2:7155521-7155540 Msa0753510:CDS 50.0%
CCTTGTCCACCAAGGACAAC+CGG + chr5_2:7152941-7152960 Msa0753510:five_prime_UTR 55.0%
!! ACCGGTTGAACCGGTTTGGT+TGG - chr5_2:7153103-7153122 None:intergenic 55.0%
CCCCGTGTCCCTTAAACATG+TGG + chr5_2:7154616-7154635 Msa0753510:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG + chr5_2:7154940-7154959 Msa0753510:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG + chr5_2:7155216-7155235 Msa0753510:CDS 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG + chr5_2:7155217-7155236 Msa0753510:CDS 55.0%
CTGTATGTCCCGGCTGGAAA+TGG - chr5_2:7155254-7155273 None:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG + chr5_2:7155296-7155315 Msa0753510:CDS 55.0%
!! GAACATAGGGCGGTGTCATG+AGG + chr5_2:7155666-7155685 Msa0753510:three_prime_UTR 55.0%
! CCGGTTGTCCTTGGTGGACA+AGG - chr5_2:7152944-7152963 None:intergenic 60.0%
! CGTACACCGGTTGTCCTTGG+TGG - chr5_2:7152950-7152969 None:intergenic 60.0%
! ACGCGTACACCGGTTGTCCT+TGG - chr5_2:7152953-7152972 None:intergenic 60.0%
AGCCACGGGCAAGCTTTGCT+TGG + chr5_2:7154047-7154066 Msa0753510:CDS 60.0%
AGCCAAGCAAAGCTTGCCCG+TGG - chr5_2:7154052-7154071 None:intergenic 60.0%
CCCGTCCACTTCAAACCACC+AGG - chr5_2:7155219-7155238 None:intergenic 60.0%
AGGGACACGGGGCCCATACT+TGG - chr5_2:7154608-7154627 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7152936 7156032 7152936 ID=Msa0753510;Name=Msa0753510
chr5_2 mRNA 7152936 7156032 7152936 ID=Msa0753510-mRNA-1;Parent=Msa0753510;Name=Msa0753510-mRNA-1;_AED=0.01;_eAED=0.01;_QI=70|1|1|1|1|1|3|416|308
chr5_2 exon 7152936 7153380 7152936 ID=Msa0753510-mRNA-1:exon:3140;Parent=Msa0753510-mRNA-1
chr5_2 exon 7154030 7154151 7154030 ID=Msa0753510-mRNA-1:exon:3141;Parent=Msa0753510-mRNA-1
chr5_2 exon 7155187 7156032 7155187 ID=Msa0753510-mRNA-1:exon:3142;Parent=Msa0753510-mRNA-1
chr5_2 five_prime_UTR 7152936 7153005 7152936 ID=Msa0753510-mRNA-1:five_prime_utr;Parent=Msa0753510-mRNA-1
chr5_2 CDS 7153006 7153380 7153006 ID=Msa0753510-mRNA-1:cds;Parent=Msa0753510-mRNA-1
chr5_2 CDS 7154030 7154151 7154030 ID=Msa0753510-mRNA-1:cds;Parent=Msa0753510-mRNA-1
chr5_2 CDS 7155187 7155616 7155187 ID=Msa0753510-mRNA-1:cds;Parent=Msa0753510-mRNA-1
chr5_2 three_prime_UTR 7155617 7156032 7155617 ID=Msa0753510-mRNA-1:three_prime_utr;Parent=Msa0753510-mRNA-1
Gene Sequence

>Msa0753510

ATGAAAACCCTTTTTCAATCTTTCTGCACCCTCTTTTCCATTATTACCATTCAATCTCAATCTTCTATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTTCAACTTTAGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGAAAGTTTGGATTGATAGAGCCACGGGCAAGCTTTGCTTGGCTATATCAGCTAACAAGGGACTATCAGTACTTGGAATTGATGATAGAAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTATCATGCAGGTGATTTCACGGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa0753510

MKTLFQSFCTLFSIITIQSQSSMGIRFSSFRSNQTGSTGSTLGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKKVWIDRATGKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*