AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1441330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1441330 MtrunA17_Chr5g0397981 97.203 286 5 1 1 286 19 301 0.0 580
Msa1441330 MtrunA17_Chr4g0057281 76.208 269 61 2 20 286 19 286 5.27e-158 441
Msa1441330 MtrunA17_Chr8g0350401 56.401 289 117 3 1 281 1 288 4.03e-119 343
Msa1441330 MtrunA17_Chr7g0265551 56.314 293 112 5 1 280 73 362 3.38e-112 328
Msa1441330 MtrunA17_Chr1g0203751 57.308 260 109 2 20 278 28 286 7.06e-112 325
Msa1441330 MtrunA17_Chr6g0472801 55.344 262 115 2 16 276 18 278 1.48e-110 321
Msa1441330 MtrunA17_Chr7g0272101 24.453 274 173 8 23 278 4 261 7.69e-28 108
Msa1441330 MtrunA17_Chr4g0010881 25.379 264 161 8 23 277 4 240 3.70e-24 97.8
Msa1441330 MtrunA17_Chr1g0189671 34.722 144 91 3 22 164 6 147 4.05e-22 93.2
Msa1441330 MtrunA17_Chr1g0189611 34.932 146 91 3 22 164 6 150 1.69e-21 91.3
Msa1441330 MtrunA17_Chr1g0189591 33.117 154 96 4 13 161 6 157 4.82e-21 90.9
Msa1441330 MtrunA17_Chr1g0189641 33.562 146 90 4 22 164 6 147 3.90e-19 85.1
Msa1441330 MtrunA17_Chr1g0189751 32.667 150 94 4 18 164 2 147 9.51e-19 84.3
Msa1441330 MtrunA17_Chr1g0189721 25.940 266 184 6 18 273 2 264 1.67e-18 83.2
Msa1441330 MtrunA17_Chr7g0272091 22.414 290 185 9 15 273 2 282 1.95e-18 83.2
Msa1441330 MtrunA17_Chr1g0189621 26.236 263 178 9 22 273 6 263 2.76e-18 82.4
Msa1441330 MtrunA17_Chr1g0189821 25.725 276 169 10 22 275 6 267 1.33e-17 80.5
Msa1441330 MtrunA17_Chr1g0203281 31.250 144 96 3 22 164 6 147 1.58e-17 80.5
Msa1441330 MtrunA17_Chr1g0190071 31.973 147 95 3 22 164 6 151 3.68e-17 79.7
Msa1441330 MtrunA17_Chr1g0192371 28.049 164 112 4 18 176 6 168 4.56e-16 76.6
Msa1441330 MtrunA17_Chr1g0189781 29.870 154 97 4 18 164 6 155 7.86e-16 75.9
Msa1441330 MtrunA17_Chr1g0189771 28.571 161 102 4 12 164 4 159 3.86e-15 72.0
Msa1441330 MtrunA17_Chr3g0097191 31.944 144 91 4 20 161 10 148 1.13e-14 72.4
Msa1441330 MtrunA17_Chr1g0203331 31.008 129 86 3 37 164 6 132 4.10e-14 70.5
Msa1441330 MtrunA17_Chr1g0189661 30.872 149 93 5 22 164 6 150 1.45e-13 69.7
Msa1441330 MtrunA17_Chr1g0189711 28.431 204 120 7 22 216 6 192 6.76e-13 67.4
Msa1441330 MtrunA17_Chr3g0095261 31.852 135 83 5 20 152 10 137 4.96e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1441330 AT1G12710.1 67.181 259 83 2 20 277 28 285 6.19e-128 365
Msa1441330 AT1G12710.2 57.807 301 83 3 20 277 28 327 7.00e-119 344
Msa1441330 AT3G61060.1 58.242 273 111 3 9 279 14 285 6.18e-118 340
Msa1441330 AT3G61060.2 58.029 274 111 4 9 279 14 286 2.18e-116 336
Msa1441330 AT1G63090.1 59.507 284 107 3 1 277 1 283 1.56e-114 332
Msa1441330 AT3G53000.1 53.333 285 120 3 1 273 1 284 1.13e-103 304
Msa1441330 AT5G52120.1 48.772 285 137 3 1 277 1 284 5.14e-98 290
Msa1441330 AT2G02360.1 34.591 159 94 4 9 164 3 154 9.10e-27 105
Msa1441330 AT2G02230.1 31.792 173 108 6 17 184 29 196 1.35e-23 98.6
Msa1441330 AT2G02240.1 34.752 141 86 3 23 162 64 199 1.65e-22 95.5
Msa1441330 AT1G09155.1 24.823 282 184 7 23 277 3 283 3.94e-22 94.0
Msa1441330 AT2G02340.1 32.927 164 100 5 18 176 34 192 4.84e-22 94.0
Msa1441330 AT2G02350.2 36.054 147 83 5 16 158 20 159 9.21e-22 92.8
Msa1441330 AT2G02250.1 30.667 150 95 3 15 161 42 185 1.75e-21 92.4
Msa1441330 AT2G02320.1 31.285 179 110 6 3 176 22 192 4.12e-20 88.6
Msa1441330 AT5G24560.1 26.347 167 112 4 22 187 5 161 1.48e-18 82.0
Msa1441330 AT2G02310.1 31.544 149 92 4 18 163 43 184 1.69e-18 84.0
Msa1441330 AT1G56250.1 24.232 293 164 8 23 273 4 280 9.79e-17 79.0
Msa1441330 AT1G56240.1 24.028 283 185 5 23 277 4 284 3.03e-16 77.4
Msa1441330 AT1G80110.1 24.242 264 181 9 20 273 1 255 5.04e-16 76.3
Msa1441330 AT2G02300.1 26.891 238 141 10 16 242 30 245 6.98e-13 67.8

Find 100 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 tig0029294:-40117 Msa1441330:CDS
CATTCAATCTCAATCTTCTA+TGG 0.161577 tig0029294:-40363 Msa1441330:exon
AATTTCTTCTGTTAATTAAA+TGG 0.179440 tig0029294:-37584 Msa1441330:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 tig0029294:+40127 Msa1441330:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 tig0029294:-37752 Msa1441330:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 tig0029294:+40275 Msa1441330:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 tig0029294:-40187 Msa1441330:CDS
GGGTGCAGAAAGATTGAAAA+AGG 0.264780 tig0029294:+40402 Msa1441330:intergenic
GGTTCAACCGGTTCAACTTT+AGG 0.271212 tig0029294:-40305 Msa1441330:CDS
TAAACTTCAGATATACTCTT+AGG 0.292898 tig0029294:+37464 Msa1441330:intergenic
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 tig0029294:+38105 Msa1441330:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 tig0029294:-40186 Msa1441330:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 tig0029294:-37824 Msa1441330:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 tig0029294:-40116 Msa1441330:CDS
ACATCAGACACAAATGTATA+TGG 0.354865 tig0029294:+37393 Msa1441330:intergenic
TTGAGATTGAATGGTAATAA+TGG 0.355846 tig0029294:+40373 Msa1441330:intergenic
TTTGTTGTGTGCAGAAAGTT+TGG 0.359355 tig0029294:-39371 Msa1441330:intron
AGCCTGTGCGATTCCAACTT+TGG 0.369551 tig0029294:-37993 Msa1441330:CDS
CGCTTCAATCATCGGTTACA+TGG 0.370713 tig0029294:-40261 Msa1441330:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 tig0029294:-37936 Msa1441330:CDS
TCTTTCTACCTGAAATGGTT+AGG 0.406400 tig0029294:+37744 Msa1441330:intergenic
TGTCCTTGGTGGACAAGGTT+TGG 0.418293 tig0029294:+40478 Msa1441330:intergenic
TTATTCTTCAGAATACATCT+AGG 0.422202 tig0029294:-38081 Msa1441330:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 tig0029294:-37508 Msa1441330:three_prime_UTR
TCCAACCAAACCGGTTCAAC+CGG 0.434266 tig0029294:-40317 Msa1441330:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 tig0029294:+40307 Msa1441330:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 tig0029294:+38053 Msa1441330:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 tig0029294:-38060 Msa1441330:CDS
CATAGAAGATTGAGATTGAA+TGG 0.442851 tig0029294:+40364 Msa1441330:intergenic
GAATTGATGATAGAAGATAT+TGG 0.446914 tig0029294:-39284 Msa1441330:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 tig0029294:-40065 Msa1441330:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 tig0029294:+40312 Msa1441330:intergenic
AGTCATAGAGAATTTGACTT+TGG 0.459733 tig0029294:+37864 Msa1441330:intergenic
CCTTGTCCACCAAGGACAAC+CGG 0.461370 tig0029294:-40473 Msa1441330:five_prime_UTR
TTTGAGTACAGTAGAATGAA+TGG 0.461971 tig0029294:-37371 Msa1441330:three_prime_UTR
ATATCATCACTGAAGAATCT+AGG 0.462039 tig0029294:-39257 Msa1441330:intron
AGCCACGGGCAAGCTTTGCT+TGG 0.464990 tig0029294:-39340 Msa1441330:CDS
TTCCAACTTTGGACTTCAGA+TGG 0.477540 tig0029294:-37982 Msa1441330:CDS
TAATTCACCTAAAGTTGAAC+CGG 0.483165 tig0029294:+40298 Msa1441330:intergenic
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 tig0029294:-40269 Msa1441330:CDS
ACGCGTACACCGGTTGTCCT+TGG 0.487127 tig0029294:+40464 Msa1441330:intergenic
TTGCATACCTTCAGCATACC+TGG 0.490977 tig0029294:-38158 Msa1441330:CDS
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 tig0029294:+38106 Msa1441330:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 tig0029294:-40326 Msa1441330:CDS
CCGGTTGTCCTTGGTGGACA+AGG 0.499332 tig0029294:+40473 Msa1441330:intergenic
TGCAACACTGAACATGTTCA+TGG 0.501094 tig0029294:-38027 Msa1441330:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 tig0029294:-38140 Msa1441330:CDS
AACAAGGGACTATCAGTACT+TGG 0.501726 tig0029294:-39306 Msa1441330:CDS
GGTGATTTCACCGTTGAGGA+TGG 0.503816 tig0029294:-37898 Msa1441330:CDS
CTTGGCTATATCAGCTAACA+AGG 0.503821 tig0029294:-39322 Msa1441330:CDS
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 tig0029294:+40316 Msa1441330:intergenic
AATGGTAATAATGGAAAAGA+GGG 0.510959 tig0029294:+40382 Msa1441330:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 tig0029294:-37838 Msa1441330:CDS
TCTAGTATGTATAGAACATA+GGG 0.518228 tig0029294:-37703 Msa1441330:three_prime_UTR
ATTCAATCTCAATCTTCTAT+GGG 0.519119 tig0029294:-40362 Msa1441330:exon
CACTGAACATGTTCATGGGT+GGG 0.526344 tig0029294:-38022 Msa1441330:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 tig0029294:+37944 Msa1441330:intergenic
CGTACACCGGTTGTCCTTGG+TGG 0.532429 tig0029294:+40467 Msa1441330:intergenic
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 tig0029294:+37980 Msa1441330:intergenic
GAATGGTAATAATGGAAAAG+AGG 0.536724 tig0029294:+40381 Msa1441330:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 tig0029294:+38140 Msa1441330:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 tig0029294:+38099 Msa1441330:intergenic
TTGACTTCCCATTTCCAGCC+GGG 0.542798 tig0029294:-38113 Msa1441330:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 tig0029294:-37981 Msa1441330:CDS
TTGAGTACAGTAGAATGAAT+GGG 0.545617 tig0029294:-37370 Msa1441330:three_prime_UTR
GCATACCTGGTGGTTTGAAG+TGG 0.546087 tig0029294:-38145 Msa1441330:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 tig0029294:+40152 Msa1441330:intergenic
GTGATTTCACCGTTGAGGAT+GGG 0.546700 tig0029294:-37897 Msa1441330:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 tig0029294:-38080 Msa1441330:CDS
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 tig0029294:-37525 Msa1441330:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 tig0029294:-38141 Msa1441330:CDS
GTCTAGTATGTATAGAACAT+AGG 0.562493 tig0029294:-37704 Msa1441330:three_prime_UTR
GTTTGGATTGATAGAGCCAC+GGG 0.562866 tig0029294:-39354 Msa1441330:CDS
ATGAATGGGAATAAGAGTGT+TGG 0.570486 tig0029294:-37356 Msa1441330:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 tig0029294:+38095 Msa1441330:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 tig0029294:+37991 Msa1441330:intergenic
TGCAGGTGATTTCACCGTTG+AGG 0.581687 tig0029294:-37902 Msa1441330:CDS
TGCGGAAGATTTGAATGTAT+GGG 0.582252 tig0029294:-37507 Msa1441330:three_prime_UTR
ATGTACAGCAACGCGTACAC+CGG 0.585734 tig0029294:+40454 Msa1441330:intergenic
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 tig0029294:-38139 Msa1441330:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 tig0029294:-38114 Msa1441330:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 tig0029294:-37690 Msa1441330:three_prime_UTR
AGTTCCTTTCAGAAAACACT+GGG 0.601469 tig0029294:+37945 Msa1441330:intergenic
AGTTTGGATTGATAGAGCCA+CGG 0.603796 tig0029294:-39355 Msa1441330:CDS
GATTGCACGCACACTAAAGG+TGG 0.628473 tig0029294:-37835 Msa1441330:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 tig0029294:-38026 Msa1441330:CDS
TGATGAATTCCCATCCTCAA+CGG 0.629667 tig0029294:+37888 Msa1441330:intergenic
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 tig0029294:-38065 Msa1441330:CDS
CAACGGTGAAATCACCTGCA+TGG 0.636855 tig0029294:+37905 Msa1441330:intergenic
TACTATTGATGATGGCACCA+AGG 0.640156 tig0029294:-40057 Msa1441330:intron
AGCCAAGCAAAGCTTGCCCG+TGG 0.641717 tig0029294:+39338 Msa1441330:intergenic
GCAAGTTGACAGATCTGAGG+AGG 0.643594 tig0029294:+40236 Msa1441330:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 tig0029294:-38023 Msa1441330:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 tig0029294:-38155 Msa1441330:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 tig0029294:+38151 Msa1441330:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 tig0029294:+37948 Msa1441330:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 tig0029294:+40233 Msa1441330:intergenic
AGTATGTATAGAACATAGGG+CGG 0.724118 tig0029294:-37700 Msa1441330:three_prime_UTR
ATACCAAACCTTGTCCACCA+AGG 0.728318 tig0029294:-40481 Msa1441330:five_prime_UTR
TTGGCTATATCAGCTAACAA+GGG 0.728498 tig0029294:-39321 Msa1441330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG - tig0029294:37965-37984 Msa1441330:CDS 10.0%
!! ACATTATAATCTATTTAATA+GGG - tig0029294:39201-39220 Msa1441330:intron 10.0%
!!! TTATTATAAATCTATTTTAG+TGG - tig0029294:39598-39617 Msa1441330:intron 10.0%
!!! TCTATCTTTTTCTATAAAAT+TGG - tig0029294:38310-38329 Msa1441330:intron 15.0%
!!! AGTTATGATATGATATTTTT+GGG - tig0029294:38811-38830 Msa1441330:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG - tig0029294:38995-39014 Msa1441330:intron 15.0%
!!! TTTTCATTAAGGAATATAAT+TGG - tig0029294:39006-39025 Msa1441330:intron 15.0%
!! CACATTATAATCTATTTAAT+AGG - tig0029294:39200-39219 Msa1441330:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG - tig0029294:40238-40257 Msa1441330:CDS 15.0%
!!! TATACCTTTAGAATCAATTT+TGG - tig0029294:38095-38114 Msa1441330:CDS 20.0%
!!! ATTGTATTTTCTAGAATAGA+TGG - tig0029294:38663-38682 Msa1441330:intron 20.0%
!!! GAGTTATGATATGATATTTT+TGG - tig0029294:38810-38829 Msa1441330:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG - tig0029294:38890-38909 Msa1441330:intron 20.0%
!!! GATATTTATTGTTTTTATCG+AGG + tig0029294:39181-39200 Msa1441330:intergenic 20.0%
!! AGAAAATCAATATGACTATT+TGG + tig0029294:39227-39246 Msa1441330:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG - tig0029294:39445-39464 Msa1441330:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG - tig0029294:40166-40185 Msa1441330:CDS 20.0%
! CATAAGAAAAAGAGAGAAAT+GGG + tig0029294:37396-37415 Msa1441330:intergenic 25.0%
! TCATAAGAAAAAGAGAGAAA+TGG + tig0029294:37397-37416 Msa1441330:intergenic 25.0%
!! AATGGTAATAATGGAAAAGA+GGG + tig0029294:37443-37462 Msa1441330:intergenic 25.0%
! TTGAGATTGAATGGTAATAA+TGG + tig0029294:37452-37471 Msa1441330:intergenic 25.0%
! ATTCAATCTCAATCTTCTAT+GGG - tig0029294:37460-37479 Msa1441330:three_prime_UTR 25.0%
!! TGGTAAATCATGAAAGATTT+TGG + tig0029294:37698-37717 Msa1441330:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG - tig0029294:37705-37724 Msa1441330:three_prime_UTR 25.0%
! CATGATTTACCAATCAATTT+GGG - tig0029294:37706-37725 Msa1441330:three_prime_UTR 25.0%
! AATCAATAAAGTAATCACCT+TGG + tig0029294:37785-37804 Msa1441330:intergenic 25.0%
!! AAATGTATGTGTGCTTATTT+TGG - tig0029294:37915-37934 Msa1441330:CDS 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG - tig0029294:38021-38040 Msa1441330:CDS 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG - tig0029294:38190-38209 Msa1441330:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG - tig0029294:38191-38210 Msa1441330:intron 25.0%
!! GAATTGATGATAGAAGATAT+TGG - tig0029294:38538-38557 Msa1441330:intron 25.0%
!!! GGACTGTCTTAATTTTTTTT+TGG - tig0029294:38684-38703 Msa1441330:intron 25.0%
!!! TTGATTAGTTTAATGATGAG+AGG - tig0029294:38740-38759 Msa1441330:intron 25.0%
!!! TGATTAGTTTAATGATGAGA+GGG - tig0029294:38741-38760 Msa1441330:intron 25.0%
! ATCTAGAAAACTATGTTTGT+GGG + tig0029294:38966-38985 Msa1441330:intergenic 25.0%
! AATCTAGAAAACTATGTTTG+TGG + tig0029294:38967-38986 Msa1441330:intergenic 25.0%
!! CAAACATAGTTTTCTAGATT+TGG - tig0029294:38967-38986 Msa1441330:intron 25.0%
!!! CGAGGTTTTTTTTTCAATTT+AGG + tig0029294:39163-39182 Msa1441330:intergenic 25.0%
!!! GATTTTCTGCATCTTTTTTA+TGG - tig0029294:39239-39258 Msa1441330:intron 25.0%
! TTATTCTTCAGAATACATCT+AGG - tig0029294:39741-39760 Msa1441330:intron 25.0%
! TATTCTTCAGAATACATCTA+GGG - tig0029294:39742-39761 Msa1441330:intron 25.0%
! TCTAGTATGTATAGAACATA+GGG - tig0029294:40119-40138 Msa1441330:CDS 25.0%
!! TGTTCTATCAAGTTTTTGTA+TGG - tig0029294:40263-40282 Msa1441330:CDS 25.0%
! TAAACTTCAGATATACTCTT+AGG + tig0029294:40361-40380 Msa1441330:intergenic 25.0%
! GAATGGTAATAATGGAAAAG+AGG + tig0029294:37444-37463 Msa1441330:intergenic 30.0%
CATAGAAGATTGAGATTGAA+TGG + tig0029294:37461-37480 Msa1441330:intergenic 30.0%
CATTCAATCTCAATCTTCTA+TGG - tig0029294:37459-37478 Msa1441330:three_prime_UTR 30.0%
TAATTCACCTAAAGTTGAAC+CGG + tig0029294:37527-37546 Msa1441330:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG + tig0029294:37718-37737 Msa1441330:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG - tig0029294:37757-37776 Msa1441330:exon 30.0%
TCCATTTCGCGAAAAATTTA+GGG + tig0029294:37838-37857 Msa1441330:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG + tig0029294:37839-37858 Msa1441330:intergenic 30.0%
!!! ATTTTGGTTTAGCGTATGTT+TGG - tig0029294:37931-37950 Msa1441330:CDS 30.0%
! TGTATGTATGATTCCACTTT+TGG + tig0029294:38126-38145 Msa1441330:intergenic 30.0%
TGGAATCATACATACACTTA+AGG - tig0029294:38130-38149 Msa1441330:CDS 30.0%
ATATCATCACTGAAGAATCT+AGG - tig0029294:38565-38584 Msa1441330:intron 30.0%
!! GATTAGTTTAATGATGAGAG+GGG - tig0029294:38742-38761 Msa1441330:intron 30.0%
TCTAGAAAACTATGTTTGTG+GGG + tig0029294:38965-38984 Msa1441330:intergenic 30.0%
GACTAACACATGTTGATATT+TGG + tig0029294:39047-39066 Msa1441330:intergenic 30.0%
GTGAAAATTGGTAAAGTGAT+AGG - tig0029294:39319-39338 Msa1441330:CDS 30.0%
GATAGGACATTGTTTGAAAA+TGG - tig0029294:39336-39355 Msa1441330:CDS 30.0%
ATCATCAACTGCGAAAATTA+AGG + tig0029294:39539-39558 Msa1441330:intergenic 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG - tig0029294:39606-39625 Msa1441330:intron 30.0%
! AGTCATAGAGAATTTGACTT+TGG + tig0029294:39961-39980 Msa1441330:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG + tig0029294:40086-40105 Msa1441330:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG - tig0029294:40118-40137 Msa1441330:CDS 30.0%
!!! ATTAGAGTTTTGTTAGAGCT+AGG - tig0029294:40189-40208 Msa1441330:CDS 30.0%
ACATCAGACACAAATGTATA+TGG + tig0029294:40432-40451 Msa1441330:intergenic 30.0%
TTTGAGTACAGTAGAATGAA+TGG - tig0029294:40451-40470 Msa1441330:five_prime_UTR 30.0%
TTGAGTACAGTAGAATGAAT+GGG - tig0029294:40452-40471 Msa1441330:five_prime_UTR 30.0%
GGTGCAGAAAGATTGAAAAA+GGG + tig0029294:37422-37441 Msa1441330:intergenic 35.0%
!! TTCTTCTGCTGATTTCGTTT+GGG - tig0029294:37636-37655 Msa1441330:three_prime_UTR 35.0%
! AGGAGAAGATGGTAATTTGT+CGG + tig0029294:37673-37692 Msa1441330:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG + tig0029294:37693-37712 Msa1441330:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG - tig0029294:37834-37853 Msa1441330:CDS 35.0%
! GGAGCCAAAATTGATTCTAA+AGG + tig0029294:38102-38121 Msa1441330:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG + tig0029294:38123-38142 Msa1441330:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG - tig0029294:38134-38153 Msa1441330:CDS 35.0%
CTTATCTCAGCTTATAACAC+AGG + tig0029294:38376-38395 Msa1441330:intergenic 35.0%
! TTTGTTGTGTGCAGAAAGTT+TGG - tig0029294:38451-38470 Msa1441330:intron 35.0%
TTGGCTATATCAGCTAACAA+GGG - tig0029294:38501-38520 Msa1441330:intron 35.0%
AACAGAATTGATCACAATGC+AGG + tig0029294:38632-38651 Msa1441330:intergenic 35.0%
!! AAGGTAATGGAGAGAACTAA+GGG - tig0029294:38765-38784 Msa1441330:intron 35.0%
GACTGGAATACATTCAAGTA+AGG - tig0029294:38907-38926 Msa1441330:intron 35.0%
AACATGTGTTAGTCCAAGTA+TGG - tig0029294:39053-39072 Msa1441330:intron 35.0%
ACATGTGTTAGTCCAAGTAT+GGG - tig0029294:39054-39073 Msa1441330:intron 35.0%
ACCTGTAACCACATGTTTAA+GGG + tig0029294:39088-39107 Msa1441330:intergenic 35.0%
AACCTGTAACCACATGTTTA+AGG + tig0029294:39089-39108 Msa1441330:intergenic 35.0%
!! CACTTGATGATGGTGAAAAT+TGG - tig0029294:39307-39326 Msa1441330:CDS 35.0%
! TTGTTTGAGTTCAACCACAT+TGG - tig0029294:39496-39515 Msa1441330:intron 35.0%
AGCAAGCATATTTACCAATG+TGG + tig0029294:39513-39532 Msa1441330:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG + tig0029294:39880-39899 Msa1441330:intergenic 35.0%
! AATTGGACTTTTTACCATGC+AGG - tig0029294:39903-39922 Msa1441330:intron 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG + tig0029294:40040-40059 Msa1441330:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG + tig0029294:40041-40060 Msa1441330:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG + tig0029294:40081-40100 Msa1441330:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG - tig0029294:40122-40141 Msa1441330:CDS 35.0%
TTGAGAAAGTTCTATGCTTG+CGG - tig0029294:40297-40316 Msa1441330:CDS 35.0%
TTGCGGAAGATTTGAATGTA+TGG - tig0029294:40314-40333 Msa1441330:CDS 35.0%
TGCGGAAGATTTGAATGTAT+GGG - tig0029294:40315-40334 Msa1441330:CDS 35.0%
! ATGAATGGGAATAAGAGTGT+TGG - tig0029294:40466-40485 Msa1441330:five_prime_UTR 35.0%
GGGTGCAGAAAGATTGAAAA+AGG + tig0029294:37423-37442 Msa1441330:intergenic 40.0%
TCATTTCGATCCAACCAAAC+CGG - tig0029294:37496-37515 Msa1441330:three_prime_UTR 40.0%
TAAAGTTGAACCGGTTGAAC+CGG + tig0029294:37518-37537 Msa1441330:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG - tig0029294:37635-37654 Msa1441330:three_prime_UTR 40.0%
!! TACTATTGATGATGGCACCA+AGG - tig0029294:37765-37784 Msa1441330:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG - tig0029294:38110-38129 Msa1441330:CDS 40.0%
! ACATACACTTAAGGCTGGTT+CGG - tig0029294:38139-38158 Msa1441330:CDS 40.0%
GATAAGCTGTTTGTCCAAAC+AGG - tig0029294:38390-38409 Msa1441330:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG - tig0029294:38398-38417 Msa1441330:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG - tig0029294:38406-38425 Msa1441330:intron 40.0%
AGTTTGGATTGATAGAGCCA+CGG - tig0029294:38467-38486 Msa1441330:intron 40.0%
CTTGGCTATATCAGCTAACA+AGG - tig0029294:38500-38519 Msa1441330:intron 40.0%
AACAAGGGACTATCAGTACT+TGG - tig0029294:38516-38535 Msa1441330:intron 40.0%
AGTTTAATGATGAGAGGGGA+AGG - tig0029294:38746-38765 Msa1441330:intron 40.0%
!! GAAGGTAATGGAGAGAACTA+AGG - tig0029294:38764-38783 Msa1441330:intron 40.0%
TAATGGAGAGAACTAAGGGA+GGG - tig0029294:38769-38788 Msa1441330:intron 40.0%
CATGTGTTAGTCCAAGTATG+GGG - tig0029294:39055-39074 Msa1441330:intron 40.0%
ATGTGTTAGTCCAAGTATGG+GGG - tig0029294:39056-39075 Msa1441330:intron 40.0%
AACCACATGTTTAAGGGACA+CGG + tig0029294:39082-39101 Msa1441330:intergenic 40.0%
TCCCTTAAACATGTGGTTAC+AGG - tig0029294:39084-39103 Msa1441330:intron 40.0%
TACAGGTTCAATTCATACCC+AGG - tig0029294:39101-39120 Msa1441330:intron 40.0%
ACAGGTTCAATTCATACCCA+GGG - tig0029294:39102-39121 Msa1441330:intron 40.0%
! TTCTTCCATCGACTAGTCAA+GGG + tig0029294:39136-39155 Msa1441330:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG + tig0029294:39137-39156 Msa1441330:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG - tig0029294:39459-39478 Msa1441330:intron 40.0%
GAAGAATAAGCTGTATGTCC+CGG + tig0029294:39730-39749 Msa1441330:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG - tig0029294:39795-39814 Msa1441330:intron 40.0%
GCAACACTGAACATGTTCAT+GGG - tig0029294:39796-39815 Msa1441330:intron 40.0%
TTCCAACTTTGGACTTCAGA+TGG - tig0029294:39840-39859 Msa1441330:intron 40.0%
TCCAACTTTGGACTTCAGAT+GGG - tig0029294:39841-39860 Msa1441330:intron 40.0%
CAGTTCCTTTCAGAAAACAC+TGG + tig0029294:39881-39900 Msa1441330:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG - tig0029294:39879-39898 Msa1441330:intron 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG - tig0029294:39886-39905 Msa1441330:intron 40.0%
TGATGAATTCCCATCCTCAA+CGG + tig0029294:39937-39956 Msa1441330:intergenic 40.0%
ATTGATTGCACGCACACTAA+AGG - tig0029294:39984-40003 Msa1441330:intron 40.0%
AAACTGCTCCTAACCATTTC+AGG - tig0029294:40070-40089 Msa1441330:CDS 40.0%
!!! AGAGTTTTGTTAGAGCTAGG+AGG - tig0029294:40192-40211 Msa1441330:CDS 40.0%
!!! GAGTTTTGTTAGAGCTAGGA+GGG - tig0029294:40193-40212 Msa1441330:CDS 40.0%
! GGTTCAACCGGTTCAACTTT+AGG - tig0029294:37517-37536 Msa1441330:three_prime_UTR 45.0%
ATTGAAGCGACGCAACTTTC+AGG + tig0029294:37550-37569 Msa1441330:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG - tig0029294:37553-37572 Msa1441330:three_prime_UTR 45.0%
! CGCTTCAATCATCGGTTACA+TGG - tig0029294:37561-37580 Msa1441330:three_prime_UTR 45.0%
! GTTGCAAGTTGACAGATCTG+AGG + tig0029294:37592-37611 Msa1441330:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG + tig0029294:37684-37703 Msa1441330:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG + tig0029294:38407-38426 Msa1441330:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG - tig0029294:38405-38424 Msa1441330:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG - tig0029294:38419-38438 Msa1441330:intron 45.0%
GTTTGGATTGATAGAGCCAC+GGG - tig0029294:38468-38487 Msa1441330:intron 45.0%
ATGATGAGAGGGGAAGGTAA+TGG - tig0029294:38752-38771 Msa1441330:intron 45.0%
! GTAATGGAGAGAACTAAGGG+AGG - tig0029294:38768-38787 Msa1441330:intron 45.0%
AATGGAGAGAACTAAGGGAG+GGG - tig0029294:38770-38789 Msa1441330:intron 45.0%
GTCAAGGGATCAATTACCCT+GGG + tig0029294:39121-39140 Msa1441330:intergenic 45.0%
AGTCAAGGGATCAATTACCC+TGG + tig0029294:39122-39141 Msa1441330:intergenic 45.0%
! TTGATCCCTTGACTAGTCGA+TGG - tig0029294:39128-39147 Msa1441330:intron 45.0%
ATCATCAAGTGCGCGAGAAT+AGG + tig0029294:39298-39317 Msa1441330:intergenic 45.0%
TATTCTCGCGCACTTGATGA+TGG - tig0029294:39297-39316 Msa1441330:CDS 45.0%
ATTACGCTTCTCTGCTACTG+AGG - tig0029294:39401-39420 Msa1441330:intron 45.0%
TTGCATACCTTCAGCATACC+TGG - tig0029294:39664-39683 Msa1441330:intron 45.0%
ACACTGAACATGTTCATGGG+TGG - tig0029294:39799-39818 Msa1441330:intron 45.0%
CACTGAACATGTTCATGGGT+GGG - tig0029294:39800-39819 Msa1441330:intron 45.0%
TCCTTTCAGAAAACACTGGG+AGG + tig0029294:39877-39896 Msa1441330:intergenic 45.0%
GTGATTTCACCGTTGAGGAT+GGG - tig0029294:39925-39944 Msa1441330:intron 45.0%
CACTAAAGGTGGTCTGTGTT+TGG - tig0029294:39998-40017 Msa1441330:intron 45.0%
! TGTCCTTGGTGGACAAGGTT+TGG + tig0029294:37347-37366 Msa0668350:intergenic 50.0%
ATGTACAGCAACGCGTACAC+CGG + tig0029294:37371-37390 Msa1441330:intergenic 50.0%
! TCCAACCAAACCGGTTCAAC+CGG - tig0029294:37505-37524 Msa1441330:three_prime_UTR 50.0%
TTGAACCGGTTGAACCGGTT+TGG + tig0029294:37513-37532 Msa1441330:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG + tig0029294:37589-37608 Msa1441330:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG - tig0029294:38144-38163 Msa1441330:CDS 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG - tig0029294:38411-38430 Msa1441330:intron 50.0%
ATGGAGAGAACTAAGGGAGG+GGG - tig0029294:38771-38790 Msa1441330:intron 50.0%
CATACCTTCAGCATACCTGG+TGG - tig0029294:39667-39686 Msa1441330:intron 50.0%
CAAACCACCAGGTATGCTGA+AGG + tig0029294:39674-39693 Msa1441330:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG - tig0029294:39677-39696 Msa1441330:intron 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG - tig0029294:39681-39700 Msa1441330:intron 50.0%
! GTTGACTTCCCATTTCCAGC+CGG - tig0029294:39708-39727 Msa1441330:intron 50.0%
TTGACTTCCCATTTCCAGCC+GGG - tig0029294:39709-39728 Msa1441330:intron 50.0%
TGTATGTCCCGGCTGGAAAT+GGG + tig0029294:39719-39738 Msa1441330:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG + tig0029294:39726-39745 Msa1441330:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG - tig0029294:39757-39776 Msa1441330:intron 50.0%
ACGACGTCCAAACCTCTTGT+AGG + tig0029294:39772-39791 Msa1441330:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG - tig0029294:39829-39848 Msa1441330:intron 50.0%
GTCCAAAGTTGGAATCGCAC+AGG + tig0029294:39834-39853 Msa1441330:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG + tig0029294:39845-39864 Msa1441330:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG - tig0029294:39873-39892 Msa1441330:intron 50.0%
CAACGGTGAAATCACCTGCA+TGG + tig0029294:39920-39939 Msa1441330:intergenic 50.0%
TGCAGGTGATTTCACCGTTG+AGG - tig0029294:39920-39939 Msa1441330:intron 50.0%
GGTGATTTCACCGTTGAGGA+TGG - tig0029294:39924-39943 Msa1441330:intron 50.0%
GATTGCACGCACACTAAAGG+TGG - tig0029294:39987-40006 Msa1441330:intron 50.0%
CCTTGTCCACCAAGGACAAC+CGG - tig0029294:37349-37368 Msa1441330:three_prime_UTR 55.0%
!! ACCGGTTGAACCGGTTTGGT+TGG + tig0029294:37509-37528 Msa1441330:intergenic 55.0%
AGAGAACTAAGGGAGGGGGA+AGG - tig0029294:38775-38794 Msa1441330:intron 55.0%
GGCCGTGTCCCTTAAACATG+TGG - tig0029294:39077-39096 Msa1441330:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG - tig0029294:39406-39425 Msa1441330:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG - tig0029294:39682-39701 Msa1441330:intron 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG - tig0029294:39683-39702 Msa1441330:intron 55.0%
CTGTATGTCCCGGCTGGAAA+TGG + tig0029294:39720-39739 Msa1441330:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG - tig0029294:39762-39781 Msa1441330:intron 55.0%
!! GAACATAGGGCGGTGTCATG+AGG - tig0029294:40132-40151 Msa1441330:CDS 55.0%
! CCGGTTGTCCTTGGTGGACA+AGG + tig0029294:37352-37371 Msa0668350:intergenic 60.0%
! CGTACACCGGTTGTCCTTGG+TGG + tig0029294:37358-37377 Msa1441330:intergenic 60.0%
! ACGCGTACACCGGTTGTCCT+TGG + tig0029294:37361-37380 Msa1441330:intergenic 60.0%
AGCCACGGGCAAGCTTTGCT+TGG - tig0029294:38482-38501 Msa1441330:intron 60.0%
AGCCAAGCAAAGCTTGCCCG+TGG + tig0029294:38487-38506 Msa1441330:intergenic 60.0%
AAGGGAGGGGGAAGGAATCG+AGG - tig0029294:38783-38802 Msa1441330:intron 60.0%
AGGGAGGGGGAAGGAATCGA+GGG - tig0029294:38784-38803 Msa1441330:intron 60.0%
AGGGGGAAGGAATCGAGGGA+TGG - tig0029294:38788-38807 Msa1441330:intron 60.0%
CCCGTCCACTTCAAACCACC+AGG + tig0029294:39685-39704 Msa1441330:intergenic 60.0%
AGGGACACGGCCCCCATACT+TGG + tig0029294:39069-39088 Msa1441330:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0029294 gene 37344 40500 37344 ID=Msa1441330;Name=Msa1441330
tig0029294 mRNA 37344 40500 37344 ID=Msa1441330-mRNA-1;Parent=Msa1441330;Name=Msa1441330-mRNA-1;_AED=0.01;_eAED=0.01;_QI=134|1|1|1|1|1|3|418|286
tig0029294 exon 37344 38191 37344 ID=Msa1441330-mRNA-1:exon:12747;Parent=Msa1441330-mRNA-1
tig0029294 exon 39258 39379 39258 ID=Msa1441330-mRNA-1:exon:12746;Parent=Msa1441330-mRNA-1
tig0029294 exon 40058 40500 40058 ID=Msa1441330-mRNA-1:exon:12745;Parent=Msa1441330-mRNA-1
tig0029294 five_prime_UTR 40367 40500 40367 ID=Msa1441330-mRNA-1:five_prime_utr;Parent=Msa1441330-mRNA-1
tig0029294 CDS 40058 40366 40058 ID=Msa1441330-mRNA-1:cds;Parent=Msa1441330-mRNA-1
tig0029294 CDS 39258 39379 39258 ID=Msa1441330-mRNA-1:cds;Parent=Msa1441330-mRNA-1
tig0029294 CDS 37762 38191 37762 ID=Msa1441330-mRNA-1:cds;Parent=Msa1441330-mRNA-1
tig0029294 three_prime_UTR 37344 37761 37344 ID=Msa1441330-mRNA-1:three_prime_utr;Parent=Msa1441330-mRNA-1
Gene Sequence

>Msa1441330

ATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTTCAACTTTAGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGAAAGTTTGGATTGATAGAGCCACGGGCAAGCTTTGCTTGGCTATATCAGCTAACAAGGGACTATCAGTACTTGGAATTGATGATAGAAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTACCATGCAGGTGATTTCACCGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa1441330

MGIRFSSFRSNQTGSTGSTLGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKKVWIDRATGKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*