AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0982550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0982550 MtrunA17_Chr5g0397981 97.203 286 5 1 1 286 19 301 0.0 580
Msa0982550 MtrunA17_Chr4g0057281 76.208 269 61 2 20 286 19 286 5.27e-158 441
Msa0982550 MtrunA17_Chr8g0350401 56.401 289 117 3 1 281 1 288 4.03e-119 343
Msa0982550 MtrunA17_Chr7g0265551 56.314 293 112 5 1 280 73 362 3.38e-112 328
Msa0982550 MtrunA17_Chr1g0203751 57.308 260 109 2 20 278 28 286 7.06e-112 325
Msa0982550 MtrunA17_Chr6g0472801 55.344 262 115 2 16 276 18 278 1.48e-110 321
Msa0982550 MtrunA17_Chr7g0272101 24.453 274 173 8 23 278 4 261 7.69e-28 108
Msa0982550 MtrunA17_Chr4g0010881 25.379 264 161 8 23 277 4 240 3.70e-24 97.8
Msa0982550 MtrunA17_Chr1g0189671 34.722 144 91 3 22 164 6 147 4.05e-22 93.2
Msa0982550 MtrunA17_Chr1g0189611 34.932 146 91 3 22 164 6 150 1.69e-21 91.3
Msa0982550 MtrunA17_Chr1g0189591 33.117 154 96 4 13 161 6 157 4.82e-21 90.9
Msa0982550 MtrunA17_Chr1g0189641 33.562 146 90 4 22 164 6 147 3.90e-19 85.1
Msa0982550 MtrunA17_Chr1g0189751 32.667 150 94 4 18 164 2 147 9.51e-19 84.3
Msa0982550 MtrunA17_Chr1g0189721 25.940 266 184 6 18 273 2 264 1.67e-18 83.2
Msa0982550 MtrunA17_Chr7g0272091 22.414 290 185 9 15 273 2 282 1.95e-18 83.2
Msa0982550 MtrunA17_Chr1g0189621 26.236 263 178 9 22 273 6 263 2.76e-18 82.4
Msa0982550 MtrunA17_Chr1g0189821 25.725 276 169 10 22 275 6 267 1.33e-17 80.5
Msa0982550 MtrunA17_Chr1g0203281 31.250 144 96 3 22 164 6 147 1.58e-17 80.5
Msa0982550 MtrunA17_Chr1g0190071 31.973 147 95 3 22 164 6 151 3.68e-17 79.7
Msa0982550 MtrunA17_Chr1g0192371 28.049 164 112 4 18 176 6 168 4.56e-16 76.6
Msa0982550 MtrunA17_Chr1g0189781 29.870 154 97 4 18 164 6 155 7.86e-16 75.9
Msa0982550 MtrunA17_Chr1g0189771 28.571 161 102 4 12 164 4 159 3.86e-15 72.0
Msa0982550 MtrunA17_Chr3g0097191 31.944 144 91 4 20 161 10 148 1.13e-14 72.4
Msa0982550 MtrunA17_Chr1g0203331 31.008 129 86 3 37 164 6 132 4.10e-14 70.5
Msa0982550 MtrunA17_Chr1g0189661 30.872 149 93 5 22 164 6 150 1.45e-13 69.7
Msa0982550 MtrunA17_Chr1g0189711 28.431 204 120 7 22 216 6 192 6.76e-13 67.4
Msa0982550 MtrunA17_Chr3g0095261 31.852 135 83 5 20 152 10 137 4.96e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0982550 AT1G12710.1 67.181 259 83 2 20 277 28 285 6.19e-128 365
Msa0982550 AT1G12710.2 57.807 301 83 3 20 277 28 327 7.00e-119 344
Msa0982550 AT3G61060.1 58.242 273 111 3 9 279 14 285 6.18e-118 340
Msa0982550 AT3G61060.2 58.029 274 111 4 9 279 14 286 2.18e-116 336
Msa0982550 AT1G63090.1 59.507 284 107 3 1 277 1 283 1.56e-114 332
Msa0982550 AT3G53000.1 53.333 285 120 3 1 273 1 284 1.13e-103 304
Msa0982550 AT5G52120.1 48.772 285 137 3 1 277 1 284 5.14e-98 290
Msa0982550 AT2G02360.1 34.591 159 94 4 9 164 3 154 9.10e-27 105
Msa0982550 AT2G02230.1 31.792 173 108 6 17 184 29 196 1.35e-23 98.6
Msa0982550 AT2G02240.1 34.752 141 86 3 23 162 64 199 1.65e-22 95.5
Msa0982550 AT1G09155.1 24.823 282 184 7 23 277 3 283 3.94e-22 94.0
Msa0982550 AT2G02340.1 32.927 164 100 5 18 176 34 192 4.84e-22 94.0
Msa0982550 AT2G02350.2 36.054 147 83 5 16 158 20 159 9.21e-22 92.8
Msa0982550 AT2G02250.1 30.667 150 95 3 15 161 42 185 1.75e-21 92.4
Msa0982550 AT2G02320.1 31.285 179 110 6 3 176 22 192 4.12e-20 88.6
Msa0982550 AT5G24560.1 26.347 167 112 4 22 187 5 161 1.48e-18 82.0
Msa0982550 AT2G02310.1 31.544 149 92 4 18 163 43 184 1.69e-18 84.0
Msa0982550 AT1G56250.1 24.232 293 164 8 23 273 4 280 9.79e-17 79.0
Msa0982550 AT1G56240.1 24.028 283 185 5 23 277 4 284 3.03e-16 77.4
Msa0982550 AT1G80110.1 24.242 264 181 9 20 273 1 255 5.04e-16 76.3
Msa0982550 AT2G02300.1 26.891 238 141 10 16 242 30 245 6.98e-13 67.8

Find 100 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 6_4:-22289803 Msa0982550:CDS
CATTCAATCTCAATCTTCTA+TGG 0.161577 6_4:-22290049 Msa0982550:exon
AATTTCTTCTGTTAATTAAA+TGG 0.179440 6_4:-22287275 Msa0982550:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 6_4:+22289813 None:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 6_4:-22287443 Msa0982550:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 6_4:+22289961 None:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 6_4:-22289873 Msa0982550:CDS
GGGTGCAGAAAGATTGAAAA+AGG 0.264780 6_4:+22290088 None:intergenic
GGTTCAACCGGTTCAACTTT+AGG 0.271212 6_4:-22289991 Msa0982550:CDS
TAAACTTCAGATATACTCTT+AGG 0.292898 6_4:+22287155 None:intergenic
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 6_4:+22287796 None:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 6_4:-22289872 Msa0982550:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 6_4:-22287515 Msa0982550:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 6_4:-22289802 Msa0982550:CDS
ACATCAGACACAAATGTATA+TGG 0.354865 6_4:+22287084 None:intergenic
TTGAGATTGAATGGTAATAA+TGG 0.355846 6_4:+22290059 None:intergenic
TTTGTTGTGTGCAGAAAGTT+TGG 0.359355 6_4:-22289061 Msa0982550:intron
AGCCTGTGCGATTCCAACTT+TGG 0.369551 6_4:-22287684 Msa0982550:CDS
CGCTTCAATCATCGGTTACA+TGG 0.370713 6_4:-22289947 Msa0982550:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 6_4:-22287627 Msa0982550:CDS
TCTTTCTACCTGAAATGGTT+AGG 0.406400 6_4:+22287435 None:intergenic
TGTCCTTGGTGGACAAGGTT+TGG 0.418293 6_4:+22290164 None:intergenic
TTATTCTTCAGAATACATCT+AGG 0.422202 6_4:-22287772 Msa0982550:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 6_4:-22287199 Msa0982550:three_prime_UTR
TCCAACCAAACCGGTTCAAC+CGG 0.434266 6_4:-22290003 Msa0982550:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 6_4:+22289993 None:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 6_4:+22287744 None:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 6_4:-22287751 Msa0982550:CDS
CATAGAAGATTGAGATTGAA+TGG 0.442851 6_4:+22290050 None:intergenic
GAATTGATGATAGAAGATAT+TGG 0.446914 6_4:-22288974 Msa0982550:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 6_4:-22289751 Msa0982550:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 6_4:+22289998 None:intergenic
AGTCATAGAGAATTTGACTT+TGG 0.459733 6_4:+22287555 None:intergenic
CCTTGTCCACCAAGGACAAC+CGG 0.461370 6_4:-22290159 Msa0982550:five_prime_UTR
TTTGAGTACAGTAGAATGAA+TGG 0.461971 6_4:-22287062 Msa0982550:three_prime_UTR
ATATCATCACTGAAGAATCT+AGG 0.462039 6_4:-22288947 Msa0982550:intron
AGCCACGGGCAAGCTTTGCT+TGG 0.464990 6_4:-22289030 Msa0982550:CDS
TTCCAACTTTGGACTTCAGA+TGG 0.477540 6_4:-22287673 Msa0982550:CDS
TAATTCACCTAAAGTTGAAC+CGG 0.483165 6_4:+22289984 None:intergenic
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 6_4:-22289955 Msa0982550:CDS
ACGCGTACACCGGTTGTCCT+TGG 0.487127 6_4:+22290150 None:intergenic
TTGCATACCTTCAGCATACC+TGG 0.490977 6_4:-22287849 Msa0982550:CDS
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 6_4:+22287797 None:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 6_4:-22290012 Msa0982550:CDS
CCGGTTGTCCTTGGTGGACA+AGG 0.499332 6_4:+22290159 None:intergenic
TGCAACACTGAACATGTTCA+TGG 0.501094 6_4:-22287718 Msa0982550:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 6_4:-22287831 Msa0982550:CDS
AACAAGGGACTATCAGTACT+TGG 0.501726 6_4:-22288996 Msa0982550:CDS
GGTGATTTCACCGTTGAGGA+TGG 0.503816 6_4:-22287589 Msa0982550:CDS
CTTGGCTATATCAGCTAACA+AGG 0.503821 6_4:-22289012 Msa0982550:CDS
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 6_4:+22290002 None:intergenic
AATGGTAATAATGGAAAAGA+GGG 0.510959 6_4:+22290068 None:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 6_4:-22287529 Msa0982550:CDS
TCTAGTATGTATAGAACATA+GGG 0.518228 6_4:-22287394 Msa0982550:three_prime_UTR
ATTCAATCTCAATCTTCTAT+GGG 0.519119 6_4:-22290048 Msa0982550:exon
CACTGAACATGTTCATGGGT+GGG 0.526344 6_4:-22287713 Msa0982550:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 6_4:+22287635 None:intergenic
CGTACACCGGTTGTCCTTGG+TGG 0.532429 6_4:+22290153 None:intergenic
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 6_4:+22287671 None:intergenic
GAATGGTAATAATGGAAAAG+AGG 0.536724 6_4:+22290067 None:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 6_4:+22287831 None:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 6_4:+22287790 None:intergenic
TTGACTTCCCATTTCCAGCC+GGG 0.542798 6_4:-22287804 Msa0982550:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 6_4:-22287672 Msa0982550:CDS
TTGAGTACAGTAGAATGAAT+GGG 0.545617 6_4:-22287061 Msa0982550:three_prime_UTR
GCATACCTGGTGGTTTGAAG+TGG 0.546087 6_4:-22287836 Msa0982550:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 6_4:+22289838 None:intergenic
GTGATTTCACCGTTGAGGAT+GGG 0.546700 6_4:-22287588 Msa0982550:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 6_4:-22287771 Msa0982550:CDS
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 6_4:-22287216 Msa0982550:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 6_4:-22287832 Msa0982550:CDS
GTCTAGTATGTATAGAACAT+AGG 0.562493 6_4:-22287395 Msa0982550:three_prime_UTR
GTTTGGATTGATAGAGCCAC+GGG 0.562866 6_4:-22289044 Msa0982550:CDS
ATGAATGGGAATAAGAGTGT+TGG 0.570486 6_4:-22287047 Msa0982550:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 6_4:+22287786 None:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 6_4:+22287682 None:intergenic
TGCAGGTGATTTCACCGTTG+AGG 0.581687 6_4:-22287593 Msa0982550:CDS
TGCGGAAGATTTGAATGTAT+GGG 0.582252 6_4:-22287198 Msa0982550:three_prime_UTR
ATGTACAGCAACGCGTACAC+CGG 0.585734 6_4:+22290140 None:intergenic
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 6_4:-22287830 Msa0982550:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 6_4:-22287805 Msa0982550:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 6_4:-22287381 Msa0982550:three_prime_UTR
AGTTCCTTTCAGAAAACACT+GGG 0.601469 6_4:+22287636 None:intergenic
AGTTTGGATTGATAGAGCCA+CGG 0.603796 6_4:-22289045 Msa0982550:CDS
GATTGCACGCACACTAAAGG+TGG 0.628473 6_4:-22287526 Msa0982550:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 6_4:-22287717 Msa0982550:CDS
TGATGAATTCCCATCCTCAA+CGG 0.629667 6_4:+22287579 None:intergenic
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 6_4:-22287756 Msa0982550:CDS
CAACGGTGAAATCACCTGCA+TGG 0.636855 6_4:+22287596 None:intergenic
TACTATTGATGATGGCACCA+AGG 0.640156 6_4:-22289743 Msa0982550:intron
AGCCAAGCAAAGCTTGCCCG+TGG 0.641717 6_4:+22289028 None:intergenic
GCAAGTTGACAGATCTGAGG+AGG 0.643594 6_4:+22289922 None:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 6_4:-22287714 Msa0982550:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 6_4:-22287846 Msa0982550:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 6_4:+22287842 None:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 6_4:+22287639 None:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 6_4:+22289919 None:intergenic
AGTATGTATAGAACATAGGG+CGG 0.724118 6_4:-22287391 Msa0982550:three_prime_UTR
ATACCAAACCTTGTCCACCA+AGG 0.728318 6_4:-22290167 Msa0982550:five_prime_UTR
TTGGCTATATCAGCTAACAA+GGG 0.728498 6_4:-22289011 Msa0982550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG - chr6_4:22287654-22287673 Msa0982550:CDS 10.0%
!! ACATTATAATCTATTTAATA+GGG - chr6_4:22288887-22288906 Msa0982550:intron 10.0%
!!! TTATTATAAATCTATTTTAG+TGG - chr6_4:22289284-22289303 Msa0982550:intron 10.0%
!!! TCTATCTTTTTCTATAAAAT+TGG - chr6_4:22287999-22288018 Msa0982550:intron 15.0%
!!! AGTTATGATATGATATTTTT+GGG - chr6_4:22288497-22288516 Msa0982550:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG - chr6_4:22288681-22288700 Msa0982550:intron 15.0%
!!! TTTTCATTAAGGAATATAAT+TGG - chr6_4:22288692-22288711 Msa0982550:intron 15.0%
!! CACATTATAATCTATTTAAT+AGG - chr6_4:22288886-22288905 Msa0982550:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG - chr6_4:22289924-22289943 Msa0982550:CDS 15.0%
!!! TATACCTTTAGAATCAATTT+TGG - chr6_4:22287784-22287803 Msa0982550:CDS 20.0%
!!! ATTGTATTTTCTAGAATAGA+TGG - chr6_4:22288350-22288369 Msa0982550:intron 20.0%
!!! GAGTTATGATATGATATTTT+TGG - chr6_4:22288496-22288515 Msa0982550:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG - chr6_4:22288576-22288595 Msa0982550:intron 20.0%
!!! GATATTTATTGTTTTTATCG+AGG + chr6_4:22288867-22288886 None:intergenic 20.0%
!! AGAAAATCAATATGACTATT+TGG + chr6_4:22288913-22288932 None:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG - chr6_4:22289131-22289150 Msa0982550:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG - chr6_4:22289852-22289871 Msa0982550:CDS 20.0%
! CATAAGAAAAAGAGAGAAAT+GGG + chr6_4:22287087-22287106 None:intergenic 25.0%
! TCATAAGAAAAAGAGAGAAA+TGG + chr6_4:22287088-22287107 None:intergenic 25.0%
!! AATGGTAATAATGGAAAAGA+GGG + chr6_4:22287134-22287153 None:intergenic 25.0%
! TTGAGATTGAATGGTAATAA+TGG + chr6_4:22287143-22287162 None:intergenic 25.0%
! ATTCAATCTCAATCTTCTAT+GGG - chr6_4:22287151-22287170 Msa0982550:three_prime_UTR 25.0%
!! TGGTAAATCATGAAAGATTT+TGG + chr6_4:22287389-22287408 None:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG - chr6_4:22287396-22287415 Msa0982550:three_prime_UTR 25.0%
! CATGATTTACCAATCAATTT+GGG - chr6_4:22287397-22287416 Msa0982550:three_prime_UTR 25.0%
! AATCAATAAAGTAATCACCT+TGG + chr6_4:22287476-22287495 None:intergenic 25.0%
!! AAATGTATGTGTGCTTATTT+TGG - chr6_4:22287604-22287623 Msa0982550:CDS 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG - chr6_4:22287710-22287729 Msa0982550:CDS 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG - chr6_4:22287879-22287898 Msa0982550:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG - chr6_4:22287880-22287899 Msa0982550:intron 25.0%
!! GAATTGATGATAGAAGATAT+TGG - chr6_4:22288225-22288244 Msa0982550:intron 25.0%
!!! TGGACTGTCTTAATTTTTTT+TGG - chr6_4:22288370-22288389 Msa0982550:intron 25.0%
!!! TTGATTAGTTTAATGATGAG+AGG - chr6_4:22288426-22288445 Msa0982550:intron 25.0%
!!! TGATTAGTTTAATGATGAGA+GGG - chr6_4:22288427-22288446 Msa0982550:intron 25.0%
! ATCTAGAAAACTATGTTTGT+GGG + chr6_4:22288652-22288671 None:intergenic 25.0%
! AATCTAGAAAACTATGTTTG+TGG + chr6_4:22288653-22288672 None:intergenic 25.0%
!! CAAACATAGTTTTCTAGATT+TGG - chr6_4:22288653-22288672 Msa0982550:intron 25.0%
!!! CGAGGTTTTTTTTTCAATTT+AGG + chr6_4:22288849-22288868 None:intergenic 25.0%
!!! GATTTTCTGCATCTTTTTTA+TGG - chr6_4:22288925-22288944 Msa0982550:intron 25.0%
! TTATTCTTCAGAATACATCT+AGG - chr6_4:22289427-22289446 Msa0982550:intron 25.0%
! TATTCTTCAGAATACATCTA+GGG - chr6_4:22289428-22289447 Msa0982550:intron 25.0%
! TCTAGTATGTATAGAACATA+GGG - chr6_4:22289805-22289824 Msa0982550:CDS 25.0%
!! TGTTCTATCAAGTTTTTGTA+TGG - chr6_4:22289949-22289968 Msa0982550:CDS 25.0%
! TAAACTTCAGATATACTCTT+AGG + chr6_4:22290047-22290066 None:intergenic 25.0%
! GAATGGTAATAATGGAAAAG+AGG + chr6_4:22287135-22287154 None:intergenic 30.0%
CATAGAAGATTGAGATTGAA+TGG + chr6_4:22287152-22287171 None:intergenic 30.0%
CATTCAATCTCAATCTTCTA+TGG - chr6_4:22287150-22287169 Msa0982550:three_prime_UTR 30.0%
TAATTCACCTAAAGTTGAAC+CGG + chr6_4:22287218-22287237 None:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG + chr6_4:22287409-22287428 None:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG - chr6_4:22287448-22287467 Msa0982550:exon 30.0%
TCCATTTCGCGAAAAATTTA+GGG + chr6_4:22287529-22287548 None:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG + chr6_4:22287530-22287549 None:intergenic 30.0%
!!! ATTTTGGTTTAGCGTATGTT+TGG - chr6_4:22287620-22287639 Msa0982550:CDS 30.0%
! TGTATGTATGATTCCACTTT+TGG + chr6_4:22287815-22287834 None:intergenic 30.0%
TGGAATCATACATACACTTA+AGG - chr6_4:22287819-22287838 Msa0982550:CDS 30.0%
ATATCATCACTGAAGAATCT+AGG - chr6_4:22288252-22288271 Msa0982550:intron 30.0%
!! GATTAGTTTAATGATGAGAG+GGG - chr6_4:22288428-22288447 Msa0982550:intron 30.0%
TCTAGAAAACTATGTTTGTG+GGG + chr6_4:22288651-22288670 None:intergenic 30.0%
GACTAACACATGTTGATATT+TGG + chr6_4:22288733-22288752 None:intergenic 30.0%
GTGAAAATTGGTAAAGTGAT+AGG - chr6_4:22289005-22289024 Msa0982550:CDS 30.0%
GATAGGACATTGTTTGAAAA+TGG - chr6_4:22289022-22289041 Msa0982550:CDS 30.0%
ATCATCAACTGCGAAAATTA+AGG + chr6_4:22289225-22289244 None:intergenic 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG - chr6_4:22289292-22289311 Msa0982550:intron 30.0%
! AGTCATAGAGAATTTGACTT+TGG + chr6_4:22289647-22289666 None:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG + chr6_4:22289772-22289791 None:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG - chr6_4:22289804-22289823 Msa0982550:CDS 30.0%
!!! ATTAGAGTTTTGTTAGAGCT+AGG - chr6_4:22289875-22289894 Msa0982550:CDS 30.0%
ACATCAGACACAAATGTATA+TGG + chr6_4:22290118-22290137 None:intergenic 30.0%
TTTGAGTACAGTAGAATGAA+TGG - chr6_4:22290137-22290156 Msa0982550:five_prime_UTR 30.0%
TTGAGTACAGTAGAATGAAT+GGG - chr6_4:22290138-22290157 Msa0982550:five_prime_UTR 30.0%
GGTGCAGAAAGATTGAAAAA+GGG + chr6_4:22287113-22287132 None:intergenic 35.0%
!! TTCTTCTGCTGATTTCGTTT+GGG - chr6_4:22287327-22287346 Msa0982550:three_prime_UTR 35.0%
! AGGAGAAGATGGTAATTTGT+CGG + chr6_4:22287364-22287383 None:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG + chr6_4:22287384-22287403 None:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG - chr6_4:22287525-22287544 Msa0982550:CDS 35.0%
! GGAGCCAAAATTGATTCTAA+AGG + chr6_4:22287791-22287810 None:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG + chr6_4:22287812-22287831 None:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG - chr6_4:22287823-22287842 Msa0982550:CDS 35.0%
CTTATCTCAGCTTATAACAC+AGG + chr6_4:22288065-22288084 None:intergenic 35.0%
! TTTGTTGTGTGCAGAAAGTT+TGG - chr6_4:22288138-22288157 Msa0982550:intron 35.0%
TTGGCTATATCAGCTAACAA+GGG - chr6_4:22288188-22288207 Msa0982550:intron 35.0%
AACAGAATTGATCACAATGC+AGG + chr6_4:22288319-22288338 None:intergenic 35.0%
!! AAGGTAATGGAGAGAACTAA+GGG - chr6_4:22288451-22288470 Msa0982550:intron 35.0%
GACTGGAATACATTCAAGTA+AGG - chr6_4:22288593-22288612 Msa0982550:intron 35.0%
AACATGTGTTAGTCCAAGTA+TGG - chr6_4:22288739-22288758 Msa0982550:intron 35.0%
ACATGTGTTAGTCCAAGTAT+GGG - chr6_4:22288740-22288759 Msa0982550:intron 35.0%
ACCTGTAACCACATGTTTAA+GGG + chr6_4:22288774-22288793 None:intergenic 35.0%
AACCTGTAACCACATGTTTA+AGG + chr6_4:22288775-22288794 None:intergenic 35.0%
!! CACTTGATGATGGTGAAAAT+TGG - chr6_4:22288993-22289012 Msa0982550:CDS 35.0%
! TTGTTTGAGTTCAACCACAT+TGG - chr6_4:22289182-22289201 Msa0982550:intron 35.0%
AGCAAGCATATTTACCAATG+TGG + chr6_4:22289199-22289218 None:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG + chr6_4:22289566-22289585 None:intergenic 35.0%
! AATTGGACTTTTTACCATGC+AGG - chr6_4:22289589-22289608 Msa0982550:intron 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG + chr6_4:22289726-22289745 None:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG + chr6_4:22289727-22289746 None:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG + chr6_4:22289767-22289786 None:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG - chr6_4:22289808-22289827 Msa0982550:CDS 35.0%
TTGAGAAAGTTCTATGCTTG+CGG - chr6_4:22289983-22290002 Msa0982550:CDS 35.0%
TTGCGGAAGATTTGAATGTA+TGG - chr6_4:22290000-22290019 Msa0982550:CDS 35.0%
TGCGGAAGATTTGAATGTAT+GGG - chr6_4:22290001-22290020 Msa0982550:CDS 35.0%
! ATGAATGGGAATAAGAGTGT+TGG - chr6_4:22290152-22290171 Msa0982550:five_prime_UTR 35.0%
GGGTGCAGAAAGATTGAAAA+AGG + chr6_4:22287114-22287133 None:intergenic 40.0%
TCATTTCGATCCAACCAAAC+CGG - chr6_4:22287187-22287206 Msa0982550:three_prime_UTR 40.0%
TAAAGTTGAACCGGTTGAAC+CGG + chr6_4:22287209-22287228 None:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG - chr6_4:22287326-22287345 Msa0982550:three_prime_UTR 40.0%
!! TACTATTGATGATGGCACCA+AGG - chr6_4:22287456-22287475 Msa0982550:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG - chr6_4:22287799-22287818 Msa0982550:CDS 40.0%
! ACATACACTTAAGGCTGGTT+CGG - chr6_4:22287828-22287847 Msa0982550:CDS 40.0%
GATAAGCTGTTTGTCCAAAC+AGG - chr6_4:22288079-22288098 Msa0982550:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG - chr6_4:22288087-22288106 Msa0982550:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG - chr6_4:22288095-22288114 Msa0982550:intron 40.0%
AGTTTGGATTGATAGAGCCA+CGG - chr6_4:22288154-22288173 Msa0982550:intron 40.0%
CTTGGCTATATCAGCTAACA+AGG - chr6_4:22288187-22288206 Msa0982550:intron 40.0%
AACAAGGGACTATCAGTACT+TGG - chr6_4:22288203-22288222 Msa0982550:intron 40.0%
AGTTTAATGATGAGAGGGGA+AGG - chr6_4:22288432-22288451 Msa0982550:intron 40.0%
!! GAAGGTAATGGAGAGAACTA+AGG - chr6_4:22288450-22288469 Msa0982550:intron 40.0%
TAATGGAGAGAACTAAGGGA+GGG - chr6_4:22288455-22288474 Msa0982550:intron 40.0%
CATGTGTTAGTCCAAGTATG+GGG - chr6_4:22288741-22288760 Msa0982550:intron 40.0%
ATGTGTTAGTCCAAGTATGG+GGG - chr6_4:22288742-22288761 Msa0982550:intron 40.0%
AACCACATGTTTAAGGGACA+CGG + chr6_4:22288768-22288787 None:intergenic 40.0%
TCCCTTAAACATGTGGTTAC+AGG - chr6_4:22288770-22288789 Msa0982550:intron 40.0%
TACAGGTTCAATTCATACCC+AGG - chr6_4:22288787-22288806 Msa0982550:intron 40.0%
ACAGGTTCAATTCATACCCA+GGG - chr6_4:22288788-22288807 Msa0982550:intron 40.0%
! TTCTTCCATCGACTAGTCAA+GGG + chr6_4:22288822-22288841 None:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG + chr6_4:22288823-22288842 None:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG - chr6_4:22289145-22289164 Msa0982550:intron 40.0%
GAAGAATAAGCTGTATGTCC+CGG + chr6_4:22289416-22289435 None:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG - chr6_4:22289481-22289500 Msa0982550:intron 40.0%
GCAACACTGAACATGTTCAT+GGG - chr6_4:22289482-22289501 Msa0982550:intron 40.0%
TTCCAACTTTGGACTTCAGA+TGG - chr6_4:22289526-22289545 Msa0982550:intron 40.0%
TCCAACTTTGGACTTCAGAT+GGG - chr6_4:22289527-22289546 Msa0982550:intron 40.0%
CAGTTCCTTTCAGAAAACAC+TGG + chr6_4:22289567-22289586 None:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG - chr6_4:22289565-22289584 Msa0982550:intron 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG - chr6_4:22289572-22289591 Msa0982550:intron 40.0%
TGATGAATTCCCATCCTCAA+CGG + chr6_4:22289623-22289642 None:intergenic 40.0%
ATTGATTGCACGCACACTAA+AGG - chr6_4:22289670-22289689 Msa0982550:intron 40.0%
AAACTGCTCCTAACCATTTC+AGG - chr6_4:22289756-22289775 Msa0982550:CDS 40.0%
!!! AGAGTTTTGTTAGAGCTAGG+AGG - chr6_4:22289878-22289897 Msa0982550:CDS 40.0%
!!! GAGTTTTGTTAGAGCTAGGA+GGG - chr6_4:22289879-22289898 Msa0982550:CDS 40.0%
! GGTTCAACCGGTTCAACTTT+AGG - chr6_4:22287208-22287227 Msa0982550:three_prime_UTR 45.0%
ATTGAAGCGACGCAACTTTC+AGG + chr6_4:22287241-22287260 None:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG - chr6_4:22287244-22287263 Msa0982550:three_prime_UTR 45.0%
! CGCTTCAATCATCGGTTACA+TGG - chr6_4:22287252-22287271 Msa0982550:three_prime_UTR 45.0%
! GTTGCAAGTTGACAGATCTG+AGG + chr6_4:22287283-22287302 None:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG + chr6_4:22287375-22287394 None:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG + chr6_4:22288096-22288115 None:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG - chr6_4:22288094-22288113 Msa0982550:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG - chr6_4:22288108-22288127 Msa0982550:intron 45.0%
GTTTGGATTGATAGAGCCAC+GGG - chr6_4:22288155-22288174 Msa0982550:intron 45.0%
ATGATGAGAGGGGAAGGTAA+TGG - chr6_4:22288438-22288457 Msa0982550:intron 45.0%
! GTAATGGAGAGAACTAAGGG+AGG - chr6_4:22288454-22288473 Msa0982550:intron 45.0%
AATGGAGAGAACTAAGGGAG+GGG - chr6_4:22288456-22288475 Msa0982550:intron 45.0%
GTCAAGGGATCAATTACCCT+GGG + chr6_4:22288807-22288826 None:intergenic 45.0%
AGTCAAGGGATCAATTACCC+TGG + chr6_4:22288808-22288827 None:intergenic 45.0%
! TTGATCCCTTGACTAGTCGA+TGG - chr6_4:22288814-22288833 Msa0982550:intron 45.0%
ATCATCAAGTGCGCGAGAAT+AGG + chr6_4:22288984-22289003 None:intergenic 45.0%
TATTCTCGCGCACTTGATGA+TGG - chr6_4:22288983-22289002 Msa0982550:CDS 45.0%
ATTACGCTTCTCTGCTACTG+AGG - chr6_4:22289087-22289106 Msa0982550:intron 45.0%
TTGCATACCTTCAGCATACC+TGG - chr6_4:22289350-22289369 Msa0982550:intron 45.0%
ACACTGAACATGTTCATGGG+TGG - chr6_4:22289485-22289504 Msa0982550:intron 45.0%
CACTGAACATGTTCATGGGT+GGG - chr6_4:22289486-22289505 Msa0982550:intron 45.0%
TCCTTTCAGAAAACACTGGG+AGG + chr6_4:22289563-22289582 None:intergenic 45.0%
GTGATTTCACCGTTGAGGAT+GGG - chr6_4:22289611-22289630 Msa0982550:intron 45.0%
CACTAAAGGTGGTCTGTGTT+TGG - chr6_4:22289684-22289703 Msa0982550:intron 45.0%
! TGTCCTTGGTGGACAAGGTT+TGG + chr6_4:22287038-22287057 None:intergenic 50.0%
ATGTACAGCAACGCGTACAC+CGG + chr6_4:22287062-22287081 None:intergenic 50.0%
! TCCAACCAAACCGGTTCAAC+CGG - chr6_4:22287196-22287215 Msa0982550:three_prime_UTR 50.0%
TTGAACCGGTTGAACCGGTT+TGG + chr6_4:22287204-22287223 None:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG + chr6_4:22287280-22287299 None:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG - chr6_4:22287833-22287852 Msa0982550:CDS 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG - chr6_4:22288100-22288119 Msa0982550:intron 50.0%
ATGGAGAGAACTAAGGGAGG+GGG - chr6_4:22288457-22288476 Msa0982550:intron 50.0%
CATACCTTCAGCATACCTGG+TGG - chr6_4:22289353-22289372 Msa0982550:intron 50.0%
CAAACCACCAGGTATGCTGA+AGG + chr6_4:22289360-22289379 None:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG - chr6_4:22289363-22289382 Msa0982550:intron 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG - chr6_4:22289367-22289386 Msa0982550:intron 50.0%
! GTTGACTTCCCATTTCCAGC+CGG - chr6_4:22289394-22289413 Msa0982550:intron 50.0%
TTGACTTCCCATTTCCAGCC+GGG - chr6_4:22289395-22289414 Msa0982550:intron 50.0%
TGTATGTCCCGGCTGGAAAT+GGG + chr6_4:22289405-22289424 None:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG + chr6_4:22289412-22289431 None:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG - chr6_4:22289443-22289462 Msa0982550:intron 50.0%
ACGACGTCCAAACCTCTTGT+AGG + chr6_4:22289458-22289477 None:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG - chr6_4:22289515-22289534 Msa0982550:intron 50.0%
GTCCAAAGTTGGAATCGCAC+AGG + chr6_4:22289520-22289539 None:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG + chr6_4:22289531-22289550 None:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG - chr6_4:22289559-22289578 Msa0982550:intron 50.0%
CAACGGTGAAATCACCTGCA+TGG + chr6_4:22289606-22289625 None:intergenic 50.0%
TGCAGGTGATTTCACCGTTG+AGG - chr6_4:22289606-22289625 Msa0982550:intron 50.0%
GGTGATTTCACCGTTGAGGA+TGG - chr6_4:22289610-22289629 Msa0982550:intron 50.0%
GATTGCACGCACACTAAAGG+TGG - chr6_4:22289673-22289692 Msa0982550:intron 50.0%
CCTTGTCCACCAAGGACAAC+CGG - chr6_4:22287040-22287059 Msa0982550:three_prime_UTR 55.0%
!! ACCGGTTGAACCGGTTTGGT+TGG + chr6_4:22287200-22287219 None:intergenic 55.0%
AGAGAACTAAGGGAGGGGGA+AGG - chr6_4:22288461-22288480 Msa0982550:intron 55.0%
GGCCGTGTCCCTTAAACATG+TGG - chr6_4:22288763-22288782 Msa0982550:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG - chr6_4:22289092-22289111 Msa0982550:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG - chr6_4:22289368-22289387 Msa0982550:intron 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG - chr6_4:22289369-22289388 Msa0982550:intron 55.0%
CTGTATGTCCCGGCTGGAAA+TGG + chr6_4:22289406-22289425 None:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG - chr6_4:22289448-22289467 Msa0982550:intron 55.0%
!! GAACATAGGGCGGTGTCATG+AGG - chr6_4:22289818-22289837 Msa0982550:CDS 55.0%
! CCGGTTGTCCTTGGTGGACA+AGG + chr6_4:22287043-22287062 None:intergenic 60.0%
! CGTACACCGGTTGTCCTTGG+TGG + chr6_4:22287049-22287068 None:intergenic 60.0%
! ACGCGTACACCGGTTGTCCT+TGG + chr6_4:22287052-22287071 None:intergenic 60.0%
AGCCACGGGCAAGCTTTGCT+TGG - chr6_4:22288169-22288188 Msa0982550:intron 60.0%
AGCCAAGCAAAGCTTGCCCG+TGG + chr6_4:22288174-22288193 None:intergenic 60.0%
AAGGGAGGGGGAAGGAATCG+AGG - chr6_4:22288469-22288488 Msa0982550:intron 60.0%
AGGGAGGGGGAAGGAATCGA+GGG - chr6_4:22288470-22288489 Msa0982550:intron 60.0%
AGGGGGAAGGAATCGAGGGA+TGG - chr6_4:22288474-22288493 Msa0982550:intron 60.0%
CCCGTCCACTTCAAACCACC+AGG + chr6_4:22289371-22289390 None:intergenic 60.0%
AGGGACACGGCCCCCATACT+TGG + chr6_4:22288755-22288774 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 22287035 22290186 22287035 ID=Msa0982550;Name=Msa0982550
chr6_4 mRNA 22287035 22290186 22287035 ID=Msa0982550-mRNA-1;Parent=Msa0982550;Name=Msa0982550-mRNA-1;_AED=0.03;_eAED=0.03;_QI=134|1|1|1|1|1|3|418|286
chr6_4 exon 22287035 22287882 22287035 ID=Msa0982550-mRNA-1:exon:7036;Parent=Msa0982550-mRNA-1
chr6_4 exon 22288948 22289069 22288948 ID=Msa0982550-mRNA-1:exon:7035;Parent=Msa0982550-mRNA-1
chr6_4 exon 22289744 22290186 22289744 ID=Msa0982550-mRNA-1:exon:7034;Parent=Msa0982550-mRNA-1
chr6_4 five_prime_UTR 22290053 22290186 22290053 ID=Msa0982550-mRNA-1:five_prime_utr;Parent=Msa0982550-mRNA-1
chr6_4 CDS 22289744 22290052 22289744 ID=Msa0982550-mRNA-1:cds;Parent=Msa0982550-mRNA-1
chr6_4 CDS 22288948 22289069 22288948 ID=Msa0982550-mRNA-1:cds;Parent=Msa0982550-mRNA-1
chr6_4 CDS 22287453 22287882 22287453 ID=Msa0982550-mRNA-1:cds;Parent=Msa0982550-mRNA-1
chr6_4 three_prime_UTR 22287035 22287452 22287035 ID=Msa0982550-mRNA-1:three_prime_utr;Parent=Msa0982550-mRNA-1
Gene Sequence

>Msa0982550

ATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTTCAACTTTAGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGAAAGTTTGGATTGATAGAGCCACGGGCAAGCTTTGCTTGGCTATATCAGCTAACAAGGGACTATCAGTACTTGGAATTGATGATAGAAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTACCATGCAGGTGATTTCACCGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa0982550

MGIRFSSFRSNQTGSTGSTLGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKKVWIDRATGKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*