AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0484400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0484400 MtrunA17_Chr5g0397981 97.203 286 5 1 1 286 19 301 0.0 580
Msa0484400 MtrunA17_Chr4g0057281 76.208 269 61 2 20 286 19 286 5.27e-158 441
Msa0484400 MtrunA17_Chr8g0350401 56.401 289 117 3 1 281 1 288 4.03e-119 343
Msa0484400 MtrunA17_Chr7g0265551 56.314 293 112 5 1 280 73 362 3.38e-112 328
Msa0484400 MtrunA17_Chr1g0203751 57.308 260 109 2 20 278 28 286 7.06e-112 325
Msa0484400 MtrunA17_Chr6g0472801 55.344 262 115 2 16 276 18 278 1.48e-110 321
Msa0484400 MtrunA17_Chr7g0272101 24.453 274 173 8 23 278 4 261 7.69e-28 108
Msa0484400 MtrunA17_Chr4g0010881 25.379 264 161 8 23 277 4 240 3.70e-24 97.8
Msa0484400 MtrunA17_Chr1g0189671 34.722 144 91 3 22 164 6 147 4.05e-22 93.2
Msa0484400 MtrunA17_Chr1g0189611 34.932 146 91 3 22 164 6 150 1.69e-21 91.3
Msa0484400 MtrunA17_Chr1g0189591 33.117 154 96 4 13 161 6 157 4.82e-21 90.9
Msa0484400 MtrunA17_Chr1g0189641 33.562 146 90 4 22 164 6 147 3.90e-19 85.1
Msa0484400 MtrunA17_Chr1g0189751 32.667 150 94 4 18 164 2 147 9.51e-19 84.3
Msa0484400 MtrunA17_Chr1g0189721 25.940 266 184 6 18 273 2 264 1.67e-18 83.2
Msa0484400 MtrunA17_Chr7g0272091 22.414 290 185 9 15 273 2 282 1.95e-18 83.2
Msa0484400 MtrunA17_Chr1g0189621 26.236 263 178 9 22 273 6 263 2.76e-18 82.4
Msa0484400 MtrunA17_Chr1g0189821 25.725 276 169 10 22 275 6 267 1.33e-17 80.5
Msa0484400 MtrunA17_Chr1g0203281 31.250 144 96 3 22 164 6 147 1.58e-17 80.5
Msa0484400 MtrunA17_Chr1g0190071 31.973 147 95 3 22 164 6 151 3.68e-17 79.7
Msa0484400 MtrunA17_Chr1g0192371 28.049 164 112 4 18 176 6 168 4.56e-16 76.6
Msa0484400 MtrunA17_Chr1g0189781 29.870 154 97 4 18 164 6 155 7.86e-16 75.9
Msa0484400 MtrunA17_Chr1g0189771 28.571 161 102 4 12 164 4 159 3.86e-15 72.0
Msa0484400 MtrunA17_Chr3g0097191 31.944 144 91 4 20 161 10 148 1.13e-14 72.4
Msa0484400 MtrunA17_Chr1g0203331 31.008 129 86 3 37 164 6 132 4.10e-14 70.5
Msa0484400 MtrunA17_Chr1g0189661 30.872 149 93 5 22 164 6 150 1.45e-13 69.7
Msa0484400 MtrunA17_Chr1g0189711 28.431 204 120 7 22 216 6 192 6.76e-13 67.4
Msa0484400 MtrunA17_Chr3g0095261 31.852 135 83 5 20 152 10 137 4.96e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0484400 AT1G12710.1 67.181 259 83 2 20 277 28 285 6.19e-128 365
Msa0484400 AT1G12710.2 57.807 301 83 3 20 277 28 327 7.00e-119 344
Msa0484400 AT3G61060.1 58.242 273 111 3 9 279 14 285 6.18e-118 340
Msa0484400 AT3G61060.2 58.029 274 111 4 9 279 14 286 2.18e-116 336
Msa0484400 AT1G63090.1 59.507 284 107 3 1 277 1 283 1.56e-114 332
Msa0484400 AT3G53000.1 53.333 285 120 3 1 273 1 284 1.13e-103 304
Msa0484400 AT5G52120.1 48.772 285 137 3 1 277 1 284 5.14e-98 290
Msa0484400 AT2G02360.1 34.591 159 94 4 9 164 3 154 9.10e-27 105
Msa0484400 AT2G02230.1 31.792 173 108 6 17 184 29 196 1.35e-23 98.6
Msa0484400 AT2G02240.1 34.752 141 86 3 23 162 64 199 1.65e-22 95.5
Msa0484400 AT1G09155.1 24.823 282 184 7 23 277 3 283 3.94e-22 94.0
Msa0484400 AT2G02340.1 32.927 164 100 5 18 176 34 192 4.84e-22 94.0
Msa0484400 AT2G02350.2 36.054 147 83 5 16 158 20 159 9.21e-22 92.8
Msa0484400 AT2G02250.1 30.667 150 95 3 15 161 42 185 1.75e-21 92.4
Msa0484400 AT2G02320.1 31.285 179 110 6 3 176 22 192 4.12e-20 88.6
Msa0484400 AT5G24560.1 26.347 167 112 4 22 187 5 161 1.48e-18 82.0
Msa0484400 AT2G02310.1 31.544 149 92 4 18 163 43 184 1.69e-18 84.0
Msa0484400 AT1G56250.1 24.232 293 164 8 23 273 4 280 9.79e-17 79.0
Msa0484400 AT1G56240.1 24.028 283 185 5 23 277 4 284 3.03e-16 77.4
Msa0484400 AT1G80110.1 24.242 264 181 9 20 273 1 255 5.04e-16 76.3
Msa0484400 AT2G02300.1 26.891 238 141 10 16 242 30 245 6.98e-13 67.8

Find 100 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 3_4:-1746605 Msa0484400:CDS
CATTCAATCTCAATCTTCTA+TGG 0.161577 3_4:-1746851 Msa0484400:exon
AATTTCTTCTGTTAATTAAA+TGG 0.179440 3_4:-1744073 Msa0484400:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 3_4:+1746615 None:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 3_4:-1744241 Msa0484400:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 3_4:+1746763 None:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 3_4:-1746675 Msa0484400:CDS
GGGTGCAGAAAGATTGAAAA+AGG 0.264780 3_4:+1746890 None:intergenic
GGTTCAACCGGTTCAACTTT+AGG 0.271212 3_4:-1746793 Msa0484400:CDS
TAAACTTCAGATATACTCTT+AGG 0.292898 3_4:+1743953 None:intergenic
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 3_4:+1744594 None:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 3_4:-1746674 Msa0484400:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 3_4:-1744313 Msa0484400:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 3_4:-1746604 Msa0484400:CDS
ACATCAGACACAAATGTATA+TGG 0.354865 3_4:+1743882 None:intergenic
TTGAGATTGAATGGTAATAA+TGG 0.355846 3_4:+1746861 None:intergenic
TTTGTTGTGTGCAGAAAGTT+TGG 0.359355 3_4:-1745865 Msa0484400:intron
AGCCTGTGCGATTCCAACTT+TGG 0.369551 3_4:-1744482 Msa0484400:CDS
CGCTTCAATCATCGGTTACA+TGG 0.370713 3_4:-1746749 Msa0484400:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 3_4:-1744425 Msa0484400:CDS
TCTTTCTACCTGAAATGGTT+AGG 0.406400 3_4:+1744233 None:intergenic
TGTCCTTGGTGGACAAGGTT+TGG 0.418293 3_4:+1746966 None:intergenic
TTATTCTTCAGAATACATCT+AGG 0.422202 3_4:-1744570 Msa0484400:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 3_4:-1743997 Msa0484400:three_prime_UTR
TCCAACCAAACCGGTTCAAC+CGG 0.434266 3_4:-1746805 Msa0484400:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 3_4:+1746795 None:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 3_4:+1744542 None:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 3_4:-1744549 Msa0484400:CDS
CATAGAAGATTGAGATTGAA+TGG 0.442851 3_4:+1746852 None:intergenic
GAATTGATGATAGAAGATAT+TGG 0.446914 3_4:-1745778 Msa0484400:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 3_4:-1746553 Msa0484400:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 3_4:+1746800 None:intergenic
AGTCATAGAGAATTTGACTT+TGG 0.459733 3_4:+1744353 None:intergenic
CCTTGTCCACCAAGGACAAC+CGG 0.461370 3_4:-1746961 Msa0484400:five_prime_UTR
TTTGAGTACAGTAGAATGAA+TGG 0.461971 3_4:-1743860 Msa0484400:three_prime_UTR
ATATCATCACTGAAGAATCT+AGG 0.462039 3_4:-1745751 Msa0484400:intron
AGCCACGGGCAAGCTTTGCT+TGG 0.464990 3_4:-1745834 Msa0484400:CDS
TTCCAACTTTGGACTTCAGA+TGG 0.477540 3_4:-1744471 Msa0484400:CDS
TAATTCACCTAAAGTTGAAC+CGG 0.483165 3_4:+1746786 None:intergenic
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 3_4:-1746757 Msa0484400:CDS
ACGCGTACACCGGTTGTCCT+TGG 0.487127 3_4:+1746952 None:intergenic
TTGCATACCTTCAGCATACC+TGG 0.490977 3_4:-1744647 Msa0484400:CDS
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 3_4:+1744595 None:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 3_4:-1746814 Msa0484400:CDS
CCGGTTGTCCTTGGTGGACA+AGG 0.499332 3_4:+1746961 None:intergenic
TGCAACACTGAACATGTTCA+TGG 0.501094 3_4:-1744516 Msa0484400:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 3_4:-1744629 Msa0484400:CDS
AACAAGGGACTATCAGTACT+TGG 0.501726 3_4:-1745800 Msa0484400:CDS
GGTGATTTCACCGTTGAGGA+TGG 0.503816 3_4:-1744387 Msa0484400:CDS
CTTGGCTATATCAGCTAACA+AGG 0.503821 3_4:-1745816 Msa0484400:CDS
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 3_4:+1746804 None:intergenic
AATGGTAATAATGGAAAAGA+GGG 0.510959 3_4:+1746870 None:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 3_4:-1744327 Msa0484400:CDS
TCTAGTATGTATAGAACATA+GGG 0.518228 3_4:-1744192 Msa0484400:three_prime_UTR
ATTCAATCTCAATCTTCTAT+GGG 0.519119 3_4:-1746850 Msa0484400:exon
CACTGAACATGTTCATGGGT+GGG 0.526344 3_4:-1744511 Msa0484400:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 3_4:+1744433 None:intergenic
CGTACACCGGTTGTCCTTGG+TGG 0.532429 3_4:+1746955 None:intergenic
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 3_4:+1744469 None:intergenic
GAATGGTAATAATGGAAAAG+AGG 0.536724 3_4:+1746869 None:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 3_4:+1744629 None:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 3_4:+1744588 None:intergenic
TTGACTTCCCATTTCCAGCC+GGG 0.542798 3_4:-1744602 Msa0484400:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 3_4:-1744470 Msa0484400:CDS
TTGAGTACAGTAGAATGAAT+GGG 0.545617 3_4:-1743859 Msa0484400:three_prime_UTR
GCATACCTGGTGGTTTGAAG+TGG 0.546087 3_4:-1744634 Msa0484400:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 3_4:+1746640 None:intergenic
GTGATTTCACCGTTGAGGAT+GGG 0.546700 3_4:-1744386 Msa0484400:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 3_4:-1744569 Msa0484400:CDS
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 3_4:-1744014 Msa0484400:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 3_4:-1744630 Msa0484400:CDS
GTCTAGTATGTATAGAACAT+AGG 0.562493 3_4:-1744193 Msa0484400:three_prime_UTR
GTTTGGATTGATAGAGCCAC+GGG 0.562866 3_4:-1745848 Msa0484400:CDS
ATGAATGGGAATAAGAGTGT+TGG 0.570486 3_4:-1743845 Msa0484400:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 3_4:+1744584 None:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 3_4:+1744480 None:intergenic
TGCAGGTGATTTCACCGTTG+AGG 0.581687 3_4:-1744391 Msa0484400:CDS
TGCGGAAGATTTGAATGTAT+GGG 0.582252 3_4:-1743996 Msa0484400:three_prime_UTR
ATGTACAGCAACGCGTACAC+CGG 0.585734 3_4:+1746942 None:intergenic
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 3_4:-1744628 Msa0484400:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 3_4:-1744603 Msa0484400:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 3_4:-1744179 Msa0484400:three_prime_UTR
AGTTCCTTTCAGAAAACACT+GGG 0.601469 3_4:+1744434 None:intergenic
AGTTTGGATTGATAGAGCCA+CGG 0.603796 3_4:-1745849 Msa0484400:CDS
GATTGCACGCACACTAAAGG+TGG 0.628473 3_4:-1744324 Msa0484400:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 3_4:-1744515 Msa0484400:CDS
TGATGAATTCCCATCCTCAA+CGG 0.629667 3_4:+1744377 None:intergenic
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 3_4:-1744554 Msa0484400:CDS
CAACGGTGAAATCACCTGCA+TGG 0.636855 3_4:+1744394 None:intergenic
TACTATTGATGATGGCACCA+AGG 0.640156 3_4:-1746545 Msa0484400:intron
AGCCAAGCAAAGCTTGCCCG+TGG 0.641717 3_4:+1745832 None:intergenic
GCAAGTTGACAGATCTGAGG+AGG 0.643594 3_4:+1746724 None:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 3_4:-1744512 Msa0484400:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 3_4:-1744644 Msa0484400:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 3_4:+1744640 None:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 3_4:+1744437 None:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 3_4:+1746721 None:intergenic
AGTATGTATAGAACATAGGG+CGG 0.724118 3_4:-1744189 Msa0484400:three_prime_UTR
ATACCAAACCTTGTCCACCA+AGG 0.728318 3_4:-1746969 Msa0484400:five_prime_UTR
TTGGCTATATCAGCTAACAA+GGG 0.728498 3_4:-1745815 Msa0484400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG - chr3_4:1744451-1744470 Msa0484400:CDS 10.0%
!! ACATTATAATCTATTTAATA+GGG - chr3_4:1745684-1745703 Msa0484400:intron 10.0%
!!! TTATTATAAATCTATTTTAG+TGG - chr3_4:1746086-1746105 Msa0484400:intron 10.0%
!!! TCTATCTTTTTCTATAAAAT+TGG - chr3_4:1744795-1744814 Msa0484400:intron 15.0%
!!! AGTTATGATATGATATTTTT+GGG - chr3_4:1745294-1745313 Msa0484400:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG - chr3_4:1745478-1745497 Msa0484400:intron 15.0%
!!! TTTTCATTAAGGAATATAAT+TGG - chr3_4:1745489-1745508 Msa0484400:intron 15.0%
!! CACATTATAATCTATTTAAT+AGG - chr3_4:1745683-1745702 Msa0484400:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG - chr3_4:1746726-1746745 Msa0484400:CDS 15.0%
!!! TATACCTTTAGAATCAATTT+TGG - chr3_4:1744581-1744600 Msa0484400:CDS 20.0%
!!! ATTGTATTTTCTAGAATAGA+TGG - chr3_4:1745146-1745165 Msa0484400:intron 20.0%
!!! GAGTTATGATATGATATTTT+TGG - chr3_4:1745293-1745312 Msa0484400:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG - chr3_4:1745373-1745392 Msa0484400:intron 20.0%
!!! GATATTTATTGTTTTTATCG+AGG + chr3_4:1745664-1745683 None:intergenic 20.0%
!! AGAAAATCAATATGACTATT+TGG + chr3_4:1745710-1745729 None:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG - chr3_4:1745928-1745947 Msa0484400:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG - chr3_4:1746654-1746673 Msa0484400:CDS 20.0%
! CATAAGAAAAAGAGAGAAAT+GGG + chr3_4:1743885-1743904 None:intergenic 25.0%
! TCATAAGAAAAAGAGAGAAA+TGG + chr3_4:1743886-1743905 None:intergenic 25.0%
!! AATGGTAATAATGGAAAAGA+GGG + chr3_4:1743932-1743951 None:intergenic 25.0%
! TTGAGATTGAATGGTAATAA+TGG + chr3_4:1743941-1743960 None:intergenic 25.0%
! ATTCAATCTCAATCTTCTAT+GGG - chr3_4:1743949-1743968 Msa0484400:three_prime_UTR 25.0%
!! TGGTAAATCATGAAAGATTT+TGG + chr3_4:1744187-1744206 None:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG - chr3_4:1744194-1744213 Msa0484400:three_prime_UTR 25.0%
! CATGATTTACCAATCAATTT+GGG - chr3_4:1744195-1744214 Msa0484400:three_prime_UTR 25.0%
! AATCAATAAAGTAATCACCT+TGG + chr3_4:1744274-1744293 None:intergenic 25.0%
!! AAATGTATGTGTGCTTATTT+TGG - chr3_4:1744401-1744420 Msa0484400:CDS 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG - chr3_4:1744507-1744526 Msa0484400:CDS 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG - chr3_4:1744676-1744695 Msa0484400:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG - chr3_4:1744677-1744696 Msa0484400:intron 25.0%
!! GAATTGATGATAGAAGATAT+TGG - chr3_4:1745021-1745040 Msa0484400:intron 25.0%
!!! GGACTGTCTTAATTTTTTTT+TGG - chr3_4:1745167-1745186 Msa0484400:intron 25.0%
!!! TTGATTAGTTTAATGATGAG+AGG - chr3_4:1745223-1745242 Msa0484400:intron 25.0%
!!! TGATTAGTTTAATGATGAGA+GGG - chr3_4:1745224-1745243 Msa0484400:intron 25.0%
! ATCTAGAAAACTATGTTTGT+GGG + chr3_4:1745449-1745468 None:intergenic 25.0%
! AATCTAGAAAACTATGTTTG+TGG + chr3_4:1745450-1745469 None:intergenic 25.0%
!! CAAACATAGTTTTCTAGATT+TGG - chr3_4:1745450-1745469 Msa0484400:intron 25.0%
!!! CGAGGTTTTTTTTTCAATTT+AGG + chr3_4:1745646-1745665 None:intergenic 25.0%
!!! GATTTTCTGCATCTTTTTTA+TGG - chr3_4:1745722-1745741 Msa0484400:intron 25.0%
! TTATTCTTCAGAATACATCT+AGG - chr3_4:1746229-1746248 Msa0484400:intron 25.0%
! TATTCTTCAGAATACATCTA+GGG - chr3_4:1746230-1746249 Msa0484400:intron 25.0%
! TCTAGTATGTATAGAACATA+GGG - chr3_4:1746607-1746626 Msa0484400:CDS 25.0%
!! TGTTCTATCAAGTTTTTGTA+TGG - chr3_4:1746751-1746770 Msa0484400:CDS 25.0%
! TAAACTTCAGATATACTCTT+AGG + chr3_4:1746849-1746868 None:intergenic 25.0%
! GAATGGTAATAATGGAAAAG+AGG + chr3_4:1743933-1743952 None:intergenic 30.0%
CATAGAAGATTGAGATTGAA+TGG + chr3_4:1743950-1743969 None:intergenic 30.0%
CATTCAATCTCAATCTTCTA+TGG - chr3_4:1743948-1743967 Msa0484400:three_prime_UTR 30.0%
TAATTCACCTAAAGTTGAAC+CGG + chr3_4:1744016-1744035 None:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG + chr3_4:1744207-1744226 None:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG - chr3_4:1744246-1744265 Msa0484400:exon 30.0%
TCCATTTCGCGAAAAATTTA+GGG + chr3_4:1744327-1744346 None:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG + chr3_4:1744328-1744347 None:intergenic 30.0%
!!! ATTTTGGTTTAGCGTATGTT+TGG - chr3_4:1744417-1744436 Msa0484400:CDS 30.0%
! TGTATGTATGATTCCACTTT+TGG + chr3_4:1744612-1744631 None:intergenic 30.0%
TGGAATCATACATACACTTA+AGG - chr3_4:1744616-1744635 Msa0484400:CDS 30.0%
ATATCATCACTGAAGAATCT+AGG - chr3_4:1745048-1745067 Msa0484400:intron 30.0%
!! GATTAGTTTAATGATGAGAG+GGG - chr3_4:1745225-1745244 Msa0484400:intron 30.0%
TCTAGAAAACTATGTTTGTG+GGG + chr3_4:1745448-1745467 None:intergenic 30.0%
GACTAACACATGTTGATATT+TGG + chr3_4:1745530-1745549 None:intergenic 30.0%
GTGAAAATTGGTAAAGTGAT+AGG - chr3_4:1745802-1745821 Msa0484400:CDS 30.0%
GATAGGACATTGTTTGAAAA+TGG - chr3_4:1745819-1745838 Msa0484400:CDS 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG - chr3_4:1746094-1746113 Msa0484400:intron 30.0%
! AGTCATAGAGAATTTGACTT+TGG + chr3_4:1746449-1746468 None:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG + chr3_4:1746574-1746593 None:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG - chr3_4:1746606-1746625 Msa0484400:CDS 30.0%
!!! ATTAGAGTTTTGTTAGAGCT+AGG - chr3_4:1746677-1746696 Msa0484400:CDS 30.0%
ACATCAGACACAAATGTATA+TGG + chr3_4:1746920-1746939 None:intergenic 30.0%
TTTGAGTACAGTAGAATGAA+TGG - chr3_4:1746939-1746958 Msa0484400:five_prime_UTR 30.0%
TTGAGTACAGTAGAATGAAT+GGG - chr3_4:1746940-1746959 Msa0484400:five_prime_UTR 30.0%
GGTGCAGAAAGATTGAAAAA+GGG + chr3_4:1743911-1743930 None:intergenic 35.0%
!! TTCTTCTGCTGATTTCGTTT+GGG - chr3_4:1744125-1744144 Msa0484400:three_prime_UTR 35.0%
! AGGAGAAGATGGTAATTTGT+CGG + chr3_4:1744162-1744181 None:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG + chr3_4:1744182-1744201 None:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG - chr3_4:1744323-1744342 Msa0484400:CDS 35.0%
! GGAGCCAAAATTGATTCTAA+AGG + chr3_4:1744588-1744607 None:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG + chr3_4:1744609-1744628 None:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG - chr3_4:1744620-1744639 Msa0484400:CDS 35.0%
CTTATCTCAGCTTATAACAC+AGG + chr3_4:1744861-1744880 None:intergenic 35.0%
! TTTGTTGTGTGCAGAAAGTT+TGG - chr3_4:1744934-1744953 Msa0484400:intron 35.0%
TTGGCTATATCAGCTAACAA+GGG - chr3_4:1744984-1745003 Msa0484400:intron 35.0%
AACAGAATTGATCACAATGC+AGG + chr3_4:1745115-1745134 None:intergenic 35.0%
!! AAGGTAATGGAGAGAACTAA+GGG - chr3_4:1745248-1745267 Msa0484400:intron 35.0%
GACTGGAATACATTCAAGTA+AGG - chr3_4:1745390-1745409 Msa0484400:intron 35.0%
AACATGTGTTAGTCCAAGTA+TGG - chr3_4:1745536-1745555 Msa0484400:intron 35.0%
ACATGTGTTAGTCCAAGTAT+GGG - chr3_4:1745537-1745556 Msa0484400:intron 35.0%
ACCTGTAACCACATGTTTAA+GGG + chr3_4:1745571-1745590 None:intergenic 35.0%
AACCTGTAACCACATGTTTA+AGG + chr3_4:1745572-1745591 None:intergenic 35.0%
!! CACTTGATGATGGTGAAAAT+TGG - chr3_4:1745790-1745809 Msa0484400:CDS 35.0%
! TTGTTTGAGTTCAACCACAT+TGG - chr3_4:1745979-1745998 Msa0484400:intron 35.0%
AGCAAGCATATTTACCAATG+TGG + chr3_4:1745996-1746015 None:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG + chr3_4:1746368-1746387 None:intergenic 35.0%
! AATTGGACTTTTTACCATGC+AGG - chr3_4:1746391-1746410 Msa0484400:intron 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG + chr3_4:1746528-1746547 None:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG + chr3_4:1746529-1746548 None:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG + chr3_4:1746569-1746588 None:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG - chr3_4:1746610-1746629 Msa0484400:CDS 35.0%
TTGAGAAAGTTCTATGCTTG+CGG - chr3_4:1746785-1746804 Msa0484400:CDS 35.0%
TTGCGGAAGATTTGAATGTA+TGG - chr3_4:1746802-1746821 Msa0484400:CDS 35.0%
TGCGGAAGATTTGAATGTAT+GGG - chr3_4:1746803-1746822 Msa0484400:CDS 35.0%
! ATGAATGGGAATAAGAGTGT+TGG - chr3_4:1746954-1746973 Msa0484400:five_prime_UTR 35.0%
GGGTGCAGAAAGATTGAAAA+AGG + chr3_4:1743912-1743931 None:intergenic 40.0%
TCATTTCGATCCAACCAAAC+CGG - chr3_4:1743985-1744004 Msa0484400:three_prime_UTR 40.0%
TAAAGTTGAACCGGTTGAAC+CGG + chr3_4:1744007-1744026 None:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG - chr3_4:1744124-1744143 Msa0484400:three_prime_UTR 40.0%
!! TACTATTGATGATGGCACCA+AGG - chr3_4:1744254-1744273 Msa0484400:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG - chr3_4:1744596-1744615 Msa0484400:CDS 40.0%
! ACATACACTTAAGGCTGGTT+CGG - chr3_4:1744625-1744644 Msa0484400:CDS 40.0%
GATAAGCTGTTTGTCCAAAC+AGG - chr3_4:1744875-1744894 Msa0484400:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG - chr3_4:1744883-1744902 Msa0484400:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG - chr3_4:1744891-1744910 Msa0484400:intron 40.0%
AGTTTGGATTGATAGAGCCA+CGG - chr3_4:1744950-1744969 Msa0484400:intron 40.0%
CTTGGCTATATCAGCTAACA+AGG - chr3_4:1744983-1745002 Msa0484400:intron 40.0%
AACAAGGGACTATCAGTACT+TGG - chr3_4:1744999-1745018 Msa0484400:intron 40.0%
AGTTTAATGATGAGAGGGGA+AGG - chr3_4:1745229-1745248 Msa0484400:intron 40.0%
!! GAAGGTAATGGAGAGAACTA+AGG - chr3_4:1745247-1745266 Msa0484400:intron 40.0%
TAATGGAGAGAACTAAGGGA+GGG - chr3_4:1745252-1745271 Msa0484400:intron 40.0%
CATGTGTTAGTCCAAGTATG+GGG - chr3_4:1745538-1745557 Msa0484400:intron 40.0%
ATGTGTTAGTCCAAGTATGG+GGG - chr3_4:1745539-1745558 Msa0484400:intron 40.0%
AACCACATGTTTAAGGGACA+CGG + chr3_4:1745565-1745584 None:intergenic 40.0%
TCCCTTAAACATGTGGTTAC+AGG - chr3_4:1745567-1745586 Msa0484400:intron 40.0%
TACAGGTTCAATTCATACCC+AGG - chr3_4:1745584-1745603 Msa0484400:intron 40.0%
ACAGGTTCAATTCATACCCA+GGG - chr3_4:1745585-1745604 Msa0484400:intron 40.0%
! TTCTTCCATCGACTAGTCAA+GGG + chr3_4:1745619-1745638 None:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG + chr3_4:1745620-1745639 None:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG - chr3_4:1745942-1745961 Msa0484400:intron 40.0%
CAACTGCACTGCGAAAATTA+AGG + chr3_4:1746022-1746041 None:intergenic 40.0%
GAAGAATAAGCTGTATGTCC+CGG + chr3_4:1746218-1746237 None:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG - chr3_4:1746283-1746302 Msa0484400:intron 40.0%
GCAACACTGAACATGTTCAT+GGG - chr3_4:1746284-1746303 Msa0484400:intron 40.0%
TTCCAACTTTGGACTTCAGA+TGG - chr3_4:1746328-1746347 Msa0484400:intron 40.0%
TCCAACTTTGGACTTCAGAT+GGG - chr3_4:1746329-1746348 Msa0484400:intron 40.0%
CAGTTCCTTTCAGAAAACAC+TGG + chr3_4:1746369-1746388 None:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG - chr3_4:1746367-1746386 Msa0484400:intron 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG - chr3_4:1746374-1746393 Msa0484400:intron 40.0%
TGATGAATTCCCATCCTCAA+CGG + chr3_4:1746425-1746444 None:intergenic 40.0%
ATTGATTGCACGCACACTAA+AGG - chr3_4:1746472-1746491 Msa0484400:intron 40.0%
AAACTGCTCCTAACCATTTC+AGG - chr3_4:1746558-1746577 Msa0484400:CDS 40.0%
!!! AGAGTTTTGTTAGAGCTAGG+AGG - chr3_4:1746680-1746699 Msa0484400:CDS 40.0%
!!! GAGTTTTGTTAGAGCTAGGA+GGG - chr3_4:1746681-1746700 Msa0484400:CDS 40.0%
! GGTTCAACCGGTTCAACTTT+AGG - chr3_4:1744006-1744025 Msa0484400:three_prime_UTR 45.0%
ATTGAAGCGACGCAACTTTC+AGG + chr3_4:1744039-1744058 None:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG - chr3_4:1744042-1744061 Msa0484400:three_prime_UTR 45.0%
! CGCTTCAATCATCGGTTACA+TGG - chr3_4:1744050-1744069 Msa0484400:three_prime_UTR 45.0%
! GTTGCAAGTTGACAGATCTG+AGG + chr3_4:1744081-1744100 None:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG + chr3_4:1744173-1744192 None:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG + chr3_4:1744892-1744911 None:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG - chr3_4:1744890-1744909 Msa0484400:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG - chr3_4:1744904-1744923 Msa0484400:intron 45.0%
GTTTGGATTGATAGAGCCAC+GGG - chr3_4:1744951-1744970 Msa0484400:intron 45.0%
ATGATGAGAGGGGAAGGTAA+TGG - chr3_4:1745235-1745254 Msa0484400:intron 45.0%
! GTAATGGAGAGAACTAAGGG+AGG - chr3_4:1745251-1745270 Msa0484400:intron 45.0%
AATGGAGAGAACTAAGGGAG+GGG - chr3_4:1745253-1745272 Msa0484400:intron 45.0%
GTCAAGGGATCAATTACCCT+GGG + chr3_4:1745604-1745623 None:intergenic 45.0%
AGTCAAGGGATCAATTACCC+TGG + chr3_4:1745605-1745624 None:intergenic 45.0%
! TTGATCCCTTGACTAGTCGA+TGG - chr3_4:1745611-1745630 Msa0484400:intron 45.0%
ATCATCAAGTGCGCGAGAAT+AGG + chr3_4:1745781-1745800 None:intergenic 45.0%
TATTCTCGCGCACTTGATGA+TGG - chr3_4:1745780-1745799 Msa0484400:CDS 45.0%
ATTACGCTTCTCTGCTACTG+AGG - chr3_4:1745884-1745903 Msa0484400:intron 45.0%
TTGCATACCTTCAGCATACC+TGG - chr3_4:1746152-1746171 Msa0484400:intron 45.0%
ACACTGAACATGTTCATGGG+TGG - chr3_4:1746287-1746306 Msa0484400:intron 45.0%
CACTGAACATGTTCATGGGT+GGG - chr3_4:1746288-1746307 Msa0484400:intron 45.0%
TCCTTTCAGAAAACACTGGG+AGG + chr3_4:1746365-1746384 None:intergenic 45.0%
GTGATTTCACCGTTGAGGAT+GGG - chr3_4:1746413-1746432 Msa0484400:intron 45.0%
CACTAAAGGTGGTCTGTGTT+TGG - chr3_4:1746486-1746505 Msa0484400:intron 45.0%
! TGTCCTTGGTGGACAAGGTT+TGG + chr3_4:1743836-1743855 None:intergenic 50.0%
ATGTACAGCAACGCGTACAC+CGG + chr3_4:1743860-1743879 None:intergenic 50.0%
! TCCAACCAAACCGGTTCAAC+CGG - chr3_4:1743994-1744013 Msa0484400:three_prime_UTR 50.0%
TTGAACCGGTTGAACCGGTT+TGG + chr3_4:1744002-1744021 None:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG + chr3_4:1744078-1744097 None:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG - chr3_4:1744630-1744649 Msa0484400:CDS 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG - chr3_4:1744896-1744915 Msa0484400:intron 50.0%
ATGGAGAGAACTAAGGGAGG+GGG - chr3_4:1745254-1745273 Msa0484400:intron 50.0%
CATACCTTCAGCATACCTGG+TGG - chr3_4:1746155-1746174 Msa0484400:intron 50.0%
CAAACCACCAGGTATGCTGA+AGG + chr3_4:1746162-1746181 None:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG - chr3_4:1746165-1746184 Msa0484400:intron 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG - chr3_4:1746169-1746188 Msa0484400:intron 50.0%
! GTTGACTTCCCATTTCCAGC+CGG - chr3_4:1746196-1746215 Msa0484400:intron 50.0%
TTGACTTCCCATTTCCAGCC+GGG - chr3_4:1746197-1746216 Msa0484400:intron 50.0%
TGTATGTCCCGGCTGGAAAT+GGG + chr3_4:1746207-1746226 None:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG + chr3_4:1746214-1746233 None:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG - chr3_4:1746245-1746264 Msa0484400:intron 50.0%
ACGACGTCCAAACCTCTTGT+AGG + chr3_4:1746260-1746279 None:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG - chr3_4:1746317-1746336 Msa0484400:intron 50.0%
GTCCAAAGTTGGAATCGCAC+AGG + chr3_4:1746322-1746341 None:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG + chr3_4:1746333-1746352 None:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG - chr3_4:1746361-1746380 Msa0484400:intron 50.0%
CAACGGTGAAATCACCTGCA+TGG + chr3_4:1746408-1746427 None:intergenic 50.0%
TGCAGGTGATTTCACCGTTG+AGG - chr3_4:1746408-1746427 Msa0484400:intron 50.0%
GGTGATTTCACCGTTGAGGA+TGG - chr3_4:1746412-1746431 Msa0484400:intron 50.0%
GATTGCACGCACACTAAAGG+TGG - chr3_4:1746475-1746494 Msa0484400:intron 50.0%
CCTTGTCCACCAAGGACAAC+CGG - chr3_4:1743838-1743857 Msa0484400:three_prime_UTR 55.0%
!! ACCGGTTGAACCGGTTTGGT+TGG + chr3_4:1743998-1744017 None:intergenic 55.0%
AGAGAACTAAGGGAGGGGGA+AGG - chr3_4:1745258-1745277 Msa0484400:intron 55.0%
GGCCGTGTCCCTTAAACATG+TGG - chr3_4:1745560-1745579 Msa0484400:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG - chr3_4:1745889-1745908 Msa0484400:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG - chr3_4:1746170-1746189 Msa0484400:intron 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG - chr3_4:1746171-1746190 Msa0484400:intron 55.0%
CTGTATGTCCCGGCTGGAAA+TGG + chr3_4:1746208-1746227 None:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG - chr3_4:1746250-1746269 Msa0484400:intron 55.0%
!! GAACATAGGGCGGTGTCATG+AGG - chr3_4:1746620-1746639 Msa0484400:CDS 55.0%
! CCGGTTGTCCTTGGTGGACA+AGG + chr3_4:1743841-1743860 None:intergenic 60.0%
! CGTACACCGGTTGTCCTTGG+TGG + chr3_4:1743847-1743866 None:intergenic 60.0%
! ACGCGTACACCGGTTGTCCT+TGG + chr3_4:1743850-1743869 None:intergenic 60.0%
AGCCACGGGCAAGCTTTGCT+TGG - chr3_4:1744965-1744984 Msa0484400:intron 60.0%
AGCCAAGCAAAGCTTGCCCG+TGG + chr3_4:1744970-1744989 None:intergenic 60.0%
AAGGGAGGGGGAAGGAATCG+AGG - chr3_4:1745266-1745285 Msa0484400:intron 60.0%
AGGGAGGGGGAAGGAATCGA+GGG - chr3_4:1745267-1745286 Msa0484400:intron 60.0%
AGGGGGAAGGAATCGAGGGA+TGG - chr3_4:1745271-1745290 Msa0484400:intron 60.0%
CCCGTCCACTTCAAACCACC+AGG + chr3_4:1746173-1746192 None:intergenic 60.0%
AGGGACACGGCCCCCATACT+TGG + chr3_4:1745552-1745571 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 1743833 1746988 1743833 ID=Msa0484400;Name=Msa0484400
chr3_4 mRNA 1743833 1746988 1743833 ID=Msa0484400-mRNA-1;Parent=Msa0484400;Name=Msa0484400-mRNA-1;_AED=0.03;_eAED=0.03;_QI=134|1|1|1|1|1|3|418|286
chr3_4 exon 1743833 1744680 1743833 ID=Msa0484400-mRNA-1:exon:532;Parent=Msa0484400-mRNA-1
chr3_4 exon 1745752 1745873 1745752 ID=Msa0484400-mRNA-1:exon:531;Parent=Msa0484400-mRNA-1
chr3_4 exon 1746546 1746988 1746546 ID=Msa0484400-mRNA-1:exon:530;Parent=Msa0484400-mRNA-1
chr3_4 five_prime_UTR 1746855 1746988 1746855 ID=Msa0484400-mRNA-1:five_prime_utr;Parent=Msa0484400-mRNA-1
chr3_4 CDS 1746546 1746854 1746546 ID=Msa0484400-mRNA-1:cds;Parent=Msa0484400-mRNA-1
chr3_4 CDS 1745752 1745873 1745752 ID=Msa0484400-mRNA-1:cds;Parent=Msa0484400-mRNA-1
chr3_4 CDS 1744251 1744680 1744251 ID=Msa0484400-mRNA-1:cds;Parent=Msa0484400-mRNA-1
chr3_4 three_prime_UTR 1743833 1744250 1743833 ID=Msa0484400-mRNA-1:three_prime_utr;Parent=Msa0484400-mRNA-1
Gene Sequence

>Msa0484400

ATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTTCAACTTTAGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGAAAGTTTGGATTGATAGAGCCACGGGCAAGCTTTGCTTGGCTATATCAGCTAACAAGGGACTATCAGTACTTGGAATTGATGATAGAAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTACCATGCAGGTGATTTCACCGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa0484400

MGIRFSSFRSNQTGSTGSTLGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKKVWIDRATGKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*