AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0761070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0761070 MtrunA17_Chr5g0411271 89.793 725 60 5 1 721 1 715 0.0 1230
Msa0761070 MtrunA17_Chr6g0458431 71.174 562 131 8 153 705 92 631 0.0 739
Msa0761070 MtrunA17_Chr6g0458441 57.967 728 194 14 18 705 1 656 0.0 715
Msa0761070 MtrunA17_Chr6g0458421 67.782 568 128 10 153 712 27 547 0.0 709
Msa0761070 MtrunA17_Chr8g0366721 56.329 316 122 4 152 466 209 509 1.01e-110 347
Msa0761070 MtrunA17_Chr8g0365831 47.516 322 155 5 152 466 161 475 5.26e-91 294
Msa0761070 MtrunA17_Chr4g0074041 42.089 316 160 6 153 455 90 395 4.89e-74 256
Msa0761070 MtrunA17_Chr4g0062561 39.375 320 173 9 141 456 239 541 4.19e-57 209
Msa0761070 MtrunA17_Chr4g0062561 38.356 292 157 8 173 456 577 853 9.25e-48 182
Msa0761070 MtrunA17_Chr4g0062491 35.806 310 178 6 166 463 1 301 6.90e-55 191
Msa0761070 MtrunA17_Chr1g0150541 35.016 317 179 9 155 455 181 486 6.15e-49 184
Msa0761070 MtrunA17_Chr3g0133061 34.286 315 181 9 156 455 585 888 3.74e-47 180
Msa0761070 MtrunA17_Chr2g0313171 34.412 340 188 11 132 455 449 769 4.55e-47 180
Msa0761070 MtrunA17_Chr5g0412381 34.024 338 194 11 133 456 405 727 8.68e-47 179
Msa0761070 MtrunA17_Chr2g0312901 32.698 315 188 7 156 455 23 328 1.82e-46 176
Msa0761070 MtrunA17_Chr5g0427301 33.651 315 182 10 156 455 543 845 1.91e-44 172
Msa0761070 MtrunA17_Chr1g0149991 31.534 352 182 11 156 456 182 525 9.98e-41 157
Msa0761070 MtrunA17_Chr8g0382161 30.259 347 191 8 156 457 24 364 1.61e-35 138
Msa0761070 MtrunA17_Chr3g0123951 30.347 346 184 10 156 453 24 360 3.11e-35 137
Msa0761070 MtrunA17_Chr4g0064671 28.400 250 120 8 258 456 5 246 1.55e-27 112
Msa0761070 MtrunA17_Chr1g0148321 29.352 293 174 9 155 430 107 383 3.43e-24 108
Msa0761070 MtrunA17_Chr3g0138791 28.424 387 243 14 60 431 17 384 1.12e-22 103
Msa0761070 MtrunA17_Chr4g0020741 26.741 359 194 12 156 455 183 531 9.23e-22 99.8
Msa0761070 MtrunA17_Chr5g0439971 26.298 289 192 9 155 431 204 483 1.48e-20 97.4
Msa0761070 MtrunA17_Chr3g0119031 26.736 288 190 10 155 430 199 477 2.04e-20 96.7
Msa0761070 MtrunA17_Chr1g0170261 26.552 290 188 10 155 430 201 479 4.02e-20 95.9
Msa0761070 MtrunA17_Chr2g0322211 33.766 154 90 5 305 455 717 861 3.72e-15 79.7
Msa0761070 MtrunA17_Chr1g0189101 35.443 158 89 6 305 458 765 913 5.01e-15 79.3
Msa0761070 MtrunA17_Chr7g0255591 32.911 158 94 6 305 458 754 903 1.92e-14 77.4
Msa0761070 MtrunA17_Chr3g0121291 35.938 128 73 3 336 455 943 1069 2.10e-14 77.4
Msa0761070 MtrunA17_Chr3g0121291 31.447 159 89 5 156 300 232 384 3.40e-12 70.1
Msa0761070 MtrunA17_Chr7g0267641 33.117 154 92 5 305 455 791 936 4.30e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0761070 AT4G17895.1 53.644 343 147 4 150 486 170 506 2.12e-114 360
Msa0761070 AT5G46740.1 48.433 351 164 3 147 486 155 499 3.29e-101 327
Msa0761070 AT5G46740.2 48.299 294 137 2 202 486 3 290 1.23e-80 266
Msa0761070 AT5G57990.1 42.540 315 159 6 153 455 105 409 3.83e-76 263
Msa0761070 AT4G31670.1 35.331 317 178 9 155 455 168 473 3.97e-49 183
Msa0761070 AT5G65450.1 34.921 315 178 9 156 455 330 632 6.00e-48 181
Msa0761070 AT5G65450.2 34.921 315 178 9 156 455 349 651 9.22e-48 181
Msa0761070 AT2G24640.1 32.877 365 211 11 107 455 133 479 2.95e-47 178
Msa0761070 AT3G14400.1 34.169 319 182 8 156 455 25 334 4.44e-47 177
Msa0761070 AT2G24640.2 34.700 317 180 9 155 455 4 309 1.14e-46 174
Msa0761070 AT1G17110.2 32.754 345 194 11 130 455 422 747 4.14e-44 171
Msa0761070 AT1G17110.3 32.754 345 194 11 130 455 418 743 4.48e-44 171
Msa0761070 AT1G17110.1 32.754 345 194 11 130 455 418 743 4.48e-44 171
Msa0761070 AT4G24560.1 31.646 316 188 10 156 455 543 846 5.04e-40 159
Msa0761070 AT2G22310.1 30.659 349 188 11 156 458 24 364 7.56e-34 134
Msa0761070 AT4G39910.1 29.944 354 187 10 156 457 24 368 2.20e-33 132
Msa0761070 AT5G10790.2 27.901 362 192 12 156 456 49 402 1.45e-29 122
Msa0761070 AT5G10790.1 27.901 362 192 12 156 456 178 531 2.18e-28 120
Msa0761070 AT2G22310.3 29.674 337 183 11 168 458 4 332 1.48e-27 114
Msa0761070 AT2G22310.2 30.508 295 157 9 206 458 39 327 1.54e-26 111
Msa0761070 AT4G30890.1 25.208 361 201 10 156 455 198 550 4.59e-23 104
Msa0761070 AT4G30890.2 25.208 361 201 10 156 455 198 550 4.59e-23 104
Msa0761070 AT4G30890.3 25.208 361 201 10 156 455 198 550 4.59e-23 104
Msa0761070 AT3G11910.3 26.829 287 193 9 155 431 198 477 2.58e-22 103
Msa0761070 AT3G11910.1 26.829 287 193 9 155 431 198 477 2.58e-22 103
Msa0761070 AT3G11910.4 26.829 287 193 9 155 431 197 476 2.83e-22 103
Msa0761070 AT3G11910.2 26.829 287 193 9 155 431 197 476 2.83e-22 103
Msa0761070 AT5G06600.1 26.689 296 200 9 155 440 199 487 1.24e-21 101
Msa0761070 AT5G06600.3 26.689 296 200 9 155 440 199 487 1.29e-21 100
Msa0761070 AT5G06600.2 26.689 296 200 9 155 440 198 486 1.29e-21 101
Msa0761070 AT3G49600.2 28.322 286 185 8 155 430 106 381 2.79e-20 96.7
Msa0761070 AT3G49600.1 28.322 286 185 8 155 430 106 381 2.79e-20 96.7
Msa0761070 AT3G49600.3 28.322 286 185 8 155 430 106 381 3.19e-20 96.7
Msa0761070 AT5G22030.1 34.416 154 90 5 305 455 723 868 2.20e-16 84.0
Msa0761070 AT5G22030.2 34.416 154 90 5 305 455 765 910 2.77e-16 84.0
Msa0761070 AT2G40930.1 33.333 156 92 6 305 456 769 916 4.28e-15 80.1
Msa0761070 AT2G40930.2 33.333 156 92 6 305 456 769 916 4.28e-15 80.1
Msa0761070 AT4G10590.4 37.419 155 84 6 305 455 748 893 5.00e-15 79.7
Msa0761070 AT4G10590.4 29.167 192 103 5 155 313 303 494 4.00e-12 70.5
Msa0761070 AT4G10590.3 37.419 155 84 6 305 455 748 893 5.00e-15 79.7
Msa0761070 AT4G10590.3 29.167 192 103 5 155 313 303 494 4.00e-12 70.5
Msa0761070 AT4G10590.1 37.419 155 84 6 305 455 748 893 5.00e-15 79.7
Msa0761070 AT4G10590.1 29.167 192 103 5 155 313 303 494 4.00e-12 70.5
Msa0761070 AT4G10590.2 37.419 155 84 6 305 455 748 893 5.00e-15 79.7
Msa0761070 AT4G10590.2 29.167 192 103 5 155 313 303 494 4.00e-12 70.5
Msa0761070 AT4G10570.1 36.774 155 85 7 305 455 749 894 2.06e-14 77.8
Msa0761070 AT4G10570.1 29.167 192 103 5 155 313 304 495 2.74e-12 70.9
Msa0761070 AT1G32850.1 35.484 155 87 7 305 455 734 879 4.31e-14 76.6
Msa0761070 AT1G32850.1 27.604 192 106 2 155 313 301 492 1.61e-12 71.6
Msa0761070 AT1G04860.1 33.974 156 86 4 156 298 232 383 1.42e-13 75.1

Find 169 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCTTAAACCTCTTTAAA+TGG 0.207305 5_2:+18059889 None:intergenic
AGTTAATTCTACTCGGATTT+TGG 0.210552 5_2:-18061524 Msa0761070:CDS
TGCTCAATTGGGTATTTCTT+TGG 0.217652 5_2:-18061734 Msa0761070:CDS
AGACTTCTCAGAAAATTCTT+TGG 0.249608 5_2:+18059052 None:intergenic
ATTGAGAGAAAGAGAAGTTT+AGG 0.251577 5_2:+18061891 None:intergenic
ATGGTGATTTATCAGATATT+TGG 0.272470 5_2:-18061585 Msa0761070:CDS
AGTTTCTGAGCCTGAGGATA+TGG 0.285559 5_2:-18061668 Msa0761070:CDS
ATTGAATAGTCTTTGACTTT+AGG 0.290779 5_2:-18058334 Msa0761070:exon
TGTCATGACTACATACAATT+TGG 0.304833 5_2:+18058234 None:intergenic
TACAATTTGGCTAGAGGTAT+TGG 0.311108 5_2:+18058247 None:intergenic
AATATTGCGAAGAAAGTCTT+TGG 0.320644 5_2:-18060246 Msa0761070:CDS
AACCGTAGATCCTGCTCAAT+TGG 0.346549 5_2:-18061746 Msa0761070:CDS
AGTCTTTGACTTTAGGCATT+TGG 0.346739 5_2:-18058327 Msa0761070:three_prime_UTR
ATCATAAACTTCATCCTTCA+AGG 0.350177 5_2:+18061426 None:intergenic
TAGGCATTTGGCTGTGTACT+TGG 0.354596 5_2:-18058315 Msa0761070:three_prime_UTR
GTATCTGTTCTCATGCATAA+TGG 0.357638 5_2:+18058197 None:intergenic
TTGGGAATTTCAGGTCATAA+TGG 0.374228 5_2:-18060661 Msa0761070:intron
AAAATCCGAGTAGAATTAAC+TGG 0.376969 5_2:+18061526 None:intergenic
ATCATCATCAGGCAAGTCTT+TGG 0.380060 5_2:-18058564 Msa0761070:CDS
GTTCTCATGCATAATGGAAC+AGG 0.386413 5_2:+18058203 None:intergenic
TCAAATGATCGCGTGAGATC+TGG 0.390392 5_2:+18058618 None:intergenic
TGGGAATTTCAGGTCATAAT+GGG 0.391890 5_2:-18060660 Msa0761070:intron
TGCCGCAAACAAGTTCCTGC+TGG 0.392129 5_2:-18058966 Msa0761070:CDS
TATGCAGTTGTTGTGCATAA+TGG 0.393951 5_2:-18059677 Msa0761070:CDS
GGCAGCTACCCTGTGGCAGC+TGG 0.397430 5_2:-18058365 Msa0761070:CDS
TATCTCCCCTGTGGTATTTG+TGG 0.399283 5_2:-18060575 Msa0761070:CDS
AGCAGTGCTCGCAAGAAATC+AGG 0.399732 5_2:-18058401 Msa0761070:CDS
GAAAGTCTTTGGAGGCAGTT+TGG 0.402179 5_2:-18060235 Msa0761070:CDS
AAATCTTCATTTGAATGGAT+TGG 0.405484 5_2:-18058928 Msa0761070:CDS
CTGCAATGAAGAAGTGTCTA+TGG 0.405794 5_2:-18059955 Msa0761070:CDS
AAACAGTCCTTGCAGTCTAC+TGG 0.408748 5_2:-18060604 Msa0761070:CDS
TTCTGATTTGATGGCCCAGC+AGG 0.410077 5_2:+18058951 None:intergenic
TCCGGTGGATACCAAGCTTC+CGG 0.412997 5_2:+18061493 None:intergenic
AAAACCAAGGTGTGCCTTGT+CGG 0.413813 5_2:+18059219 None:intergenic
GTTTCTGAGCCTGAGGATAT+GGG 0.417439 5_2:-18061667 Msa0761070:CDS
GTGAATTATGCCAGCCCGAC+TGG 0.419613 5_2:-18058879 Msa0761070:CDS
CGAGTAGAATTAACTGGAAC+CGG 0.424615 5_2:+18061532 None:intergenic
TCCAACTTATGCCATGTGTC+TGG 0.425693 5_2:+18059608 None:intergenic
TACAACCAGTTCTCATCATC+AGG 0.433784 5_2:+18061463 None:intergenic
GACGTTTATGTTGATGTTGT+CGG 0.434418 5_2:-18061691 Msa0761070:CDS
TGGCTGTGCCGCCGGAAGCT+TGG 0.438381 5_2:-18061504 Msa0761070:CDS
TCCTCTTACTCACCATCATT+AGG 0.442960 5_2:+18060809 None:intergenic
AAATAGAAAATGATTTATCC+TGG 0.443696 5_2:+18059111 None:intergenic
CTTTGCAATGCCAGTCGGGC+TGG 0.444918 5_2:+18058869 None:intergenic
CGGTTCCAGTTAATTCTACT+CGG 0.445652 5_2:-18061531 Msa0761070:CDS
GCTGCTGTCTTTGTCCGACT+TGG 0.449405 5_2:+18058421 None:intergenic
GGAGCTGGGCTTGCCAACCT+TGG 0.457605 5_2:-18060917 Msa0761070:CDS
AAATGGAATGCAGCAATTGA+AGG 0.458769 5_2:+18059906 None:intergenic
CAGGCTTTGCAATGCCAGTC+GGG 0.460520 5_2:+18058865 None:intergenic
AAGCTGCCAGGGCATATGTT+AGG 0.462405 5_2:-18058526 Msa0761070:CDS
GGTAATTATTCAGGTAACTA+AGG 0.467491 5_2:-18059291 Msa0761070:intron
TGAAGGGACTGTGCTGTCTC+AGG 0.467824 5_2:-18059264 Msa0761070:CDS
TATCAATTTCCCACTGGAGT+TGG 0.469397 5_2:-18059835 Msa0761070:CDS
GATTTATCAGATATTTGGCC+GGG 0.469571 5_2:-18061580 Msa0761070:CDS
AATGGAACTTCATCTGACTC+TGG 0.470300 5_2:-18059659 Msa0761070:CDS
AAACCAAGGTGTGCCTTGTC+GGG 0.471326 5_2:+18059220 None:intergenic
CATAATTCACAGGTAGCACT+TGG 0.473153 5_2:+18058892 None:intergenic
ACCGTAGATCCTGCTCAATT+GGG 0.475265 5_2:-18061745 Msa0761070:CDS
AGCAGCCATTCTCCTAATGA+TGG 0.479019 5_2:-18060821 Msa0761070:intron
AATATTGAAAAGAGTGGGGC+TGG 0.480181 5_2:-18059080 Msa0761070:CDS
TTGCTGCATTCCATTTAAAG+AGG 0.480468 5_2:-18059899 Msa0761070:CDS
TCAAAAGAAGACCCTCAACT+AGG 0.481413 5_2:+18060843 None:intergenic
TTTAGACATCATTGATGGCC+AGG 0.481820 5_2:-18059129 Msa0761070:CDS
AGATATTTGGCCGGGGCCAA+TGG 0.483102 5_2:-18061572 Msa0761070:CDS
AATGAAAATATTGAAAAGAG+TGG 0.488221 5_2:-18059086 Msa0761070:CDS
ATGAAAATATTGAAAAGAGT+GGG 0.488375 5_2:-18059085 Msa0761070:CDS
GAAGAAATGAAATTCTTGGT+TGG 0.491050 5_2:+18061919 None:intergenic
ATCTCGCTGCGGAACATCAC+CGG 0.496487 5_2:+18061609 None:intergenic
CAAAAGAAGACCCTCAACTA+GGG 0.500648 5_2:+18060844 None:intergenic
GCCCAGCAGGAACTTGTTTG+CGG 0.500874 5_2:+18058964 None:intergenic
GCATACTGTGCCCCTAGTTG+AGG 0.501299 5_2:-18060855 Msa0761070:CDS
AGATACTATTTCAAGTGCAT+TGG 0.502024 5_2:-18060027 Msa0761070:CDS
GCATACACAGCAAGATCCAT+TGG 0.503857 5_2:+18061556 None:intergenic
ATCCTCAAAATAAAGCTGCC+AGG 0.504847 5_2:-18058538 Msa0761070:CDS
GATGTTCCGCAGCGAGATCA+TGG 0.509643 5_2:-18061604 Msa0761070:CDS
GCATAAGTTGGATGATTCAA+TGG 0.511129 5_2:-18059597 Msa0761070:CDS
CCGACTGGCATTGCAAAGCC+TGG 0.513078 5_2:-18058864 Msa0761070:CDS
ATGCTCCTGATGATGAGAAC+TGG 0.515150 5_2:-18061468 Msa0761070:CDS
AGTGGTAAAAGATCATCATC+AGG 0.517890 5_2:-18058575 Msa0761070:CDS
TCATCATCAGGAGCATAATC+CGG 0.519460 5_2:+18061475 None:intergenic
CCAGGCTTTGCAATGCCAGT+CGG 0.519525 5_2:+18058864 None:intergenic
TGTGAAACTCCAGCTGCCAC+AGG 0.523773 5_2:+18058356 None:intergenic
ACTCACCATCATTAGGAGAA+TGG 0.525951 5_2:+18060816 None:intergenic
GCCGCAAACAAGTTCCTGCT+GGG 0.528262 5_2:-18058965 Msa0761070:CDS
TGGAGGCAGTTTGGTTAGCA+AGG 0.528538 5_2:-18060226 Msa0761070:intron
TGAGTTTATGCAGTGTGCAT+TGG 0.528922 5_2:-18060307 Msa0761070:CDS
CAATCACCTGGAGTCTGAGA+AGG 0.530275 5_2:+18058729 None:intergenic
TTAGGAATATGCCTGGTTCA+AGG 0.531245 5_2:-18058508 Msa0761070:CDS
CATACTGTGCCCCTAGTTGA+GGG 0.532476 5_2:-18060854 Msa0761070:CDS
GTCGGGCTGGCATAATTCAC+AGG 0.532843 5_2:+18058882 None:intergenic
AAAACGCTTACAATCGTCAA+TGG 0.544488 5_2:+18061385 None:intergenic
CTGGCATTGCAAAGCCTGGT+GGG 0.544914 5_2:-18058860 Msa0761070:CDS
AATCACCTGGAGTCTGAGAA+GGG 0.545095 5_2:+18058730 None:intergenic
ACCCAATTGAGCAGGATCTA+CGG 0.547215 5_2:+18061744 None:intergenic
TTGGTTAACCCCATATCCTC+AGG 0.548294 5_2:+18061658 None:intergenic
GGTAGCTGCCGACGGAAGCA+TGG 0.550788 5_2:+18058378 None:intergenic
TCTAGACTCAGGTCAATCAA+TGG 0.552026 5_2:+18060062 None:intergenic
GCCGGAAGCTTGGTATCCAC+CGG 0.552170 5_2:-18061494 Msa0761070:CDS
CAGGTAACTAAGGTATCTGA+AGG 0.552413 5_2:-18059281 Msa0761070:CDS
TATGGAGAAGCAGCTTATGT+TGG 0.552768 5_2:-18059937 Msa0761070:CDS
ATGGAACTTCATCTGACTCT+GGG 0.557189 5_2:-18059658 Msa0761070:CDS
ACAGAGAAGCAAGGAAGTAG+TGG 0.558974 5_2:-18058593 Msa0761070:CDS
ACCTGGAGTCTGAGAAGGGG+GGG 0.559134 5_2:+18058734 None:intergenic
TCCAGACACATGGCATAAGT+TGG 0.561978 5_2:-18059609 Msa0761070:CDS
TGCCACAGGGTAGCTGCCGA+CGG 0.563195 5_2:+18058370 None:intergenic
AATGATGTCTAAAACAGACT+TGG 0.564405 5_2:+18059139 None:intergenic
TGATTTATCAGATATTTGGC+CGG 0.566794 5_2:-18061581 Msa0761070:CDS
TCAGCATATGAACTCCCACC+AGG 0.567820 5_2:+18058846 None:intergenic
GCAGGAACTTGTTTGCGGCA+AGG 0.568113 5_2:+18058969 None:intergenic
TCACCTGGAGTCTGAGAAGG+GGG 0.569077 5_2:+18058732 None:intergenic
TCCTCAAAATAAAGCTGCCA+GGG 0.569220 5_2:-18058537 Msa0761070:CDS
AAATTATCCACAAATACCAC+AGG 0.569292 5_2:+18060568 None:intergenic
AAAGAAATACCCAATTGAGC+AGG 0.570044 5_2:+18061736 None:intergenic
ATTGCGAAGAAAGTCTTTGG+AGG 0.579484 5_2:-18060243 Msa0761070:CDS
CACGGACATAAACTCATCGT+TGG 0.580359 5_2:+18061639 None:intergenic
ACAGCAAGATCCATTGGCCC+CGG 0.580619 5_2:+18061562 None:intergenic
CATATTCCTAACATATGCCC+TGG 0.580915 5_2:+18058520 None:intergenic
GCATATGTTAGGAATATGCC+TGG 0.584399 5_2:-18058515 Msa0761070:CDS
CAAGCATATCAATTTCCCAC+TGG 0.585157 5_2:-18059841 Msa0761070:CDS
ATGATGTCTAAAACAGACTT+GGG 0.586058 5_2:+18059140 None:intergenic
TTCGCACTGCTCCAGACACA+TGG 0.586738 5_2:-18059619 Msa0761070:CDS
TTTCTGAGCCTGAGGATATG+GGG 0.586760 5_2:-18061666 Msa0761070:CDS
AATGCACCTCTCCTTGAACC+AGG 0.588351 5_2:+18058497 None:intergenic
AACTCTTCCAGTAGACTGCA+AGG 0.591622 5_2:+18060597 None:intergenic
TGCATTGGAGTCTTTCACAA+TGG 0.593024 5_2:-18060012 Msa0761070:CDS
ACAAATAGAAATCGTGGACA+AGG 0.593108 5_2:-18058176 Msa0761070:three_prime_UTR
TGAAAATATTGAAAAGAGTG+GGG 0.594431 5_2:-18059084 Msa0761070:CDS
AAAGCATGTGTTGCCAAGGT+TGG 0.598808 5_2:+18060904 None:intergenic
AACATCACCGGTGTGCATCA+CGG 0.599073 5_2:+18061621 None:intergenic
ATTGGAGTCTTTCACAATGG+TGG 0.605716 5_2:-18060009 Msa0761070:CDS
ATGCCCGACAAGGCACACCT+TGG 0.606806 5_2:-18059223 Msa0761070:CDS
ACAGATACAAATAGAAATCG+TGG 0.607957 5_2:-18058182 Msa0761070:three_prime_UTR
GGATCTTGCTGTGTATGCTC+CGG 0.610441 5_2:-18061551 Msa0761070:CDS
TGATGCTAAGATTAAATGTG+AGG 0.612633 5_2:-18059979 Msa0761070:CDS
ATCACCTGGAGTCTGAGAAG+GGG 0.616978 5_2:+18058731 None:intergenic
ATTTATCAGATATTTGGCCG+GGG 0.621370 5_2:-18061579 Msa0761070:CDS
TGAGAAAGCATGTGTTGCCA+AGG 0.622674 5_2:+18060900 None:intergenic
GGTGAAAATACCTTTATCTG+TGG 0.622911 5_2:-18058284 Msa0761070:three_prime_UTR
AAAGACTGATTCATCCAAGT+CGG 0.626726 5_2:-18058435 Msa0761070:CDS
GCTCATTAGTGTCAGAAGAG+TGG 0.628120 5_2:+18060084 None:intergenic
AAATCAGGCCATGCTTCCGT+CGG 0.629660 5_2:-18058386 Msa0761070:CDS
GTGAAACTCCAGCTGCCACA+GGG 0.630296 5_2:+18058357 None:intergenic
ATCCTACTCAGATTCAAGCA+TGG 0.632686 5_2:+18059173 None:intergenic
AATATGCCTGGTTCAAGGAG+AGG 0.638632 5_2:-18058503 Msa0761070:CDS
GGTTGCAAATCCAACTCCAG+TGG 0.642634 5_2:+18059825 None:intergenic
TTCCATGCTTGAATCTGAGT+AGG 0.644297 5_2:-18059175 Msa0761070:CDS
ATAATTCACAGGTAGCACTT+GGG 0.648379 5_2:+18058893 None:intergenic
AAAAGAAGACCCTCAACTAG+GGG 0.651897 5_2:+18060845 None:intergenic
TGTCGGAGTTTCTGAGCCTG+AGG 0.652877 5_2:-18061674 Msa0761070:CDS
ACTACATACAATTTGGCTAG+AGG 0.656761 5_2:+18058241 None:intergenic
TTCTCATGCATAATGGAACA+GGG 0.661403 5_2:+18058204 None:intergenic
TTCCGTCGGCAGCTACCCTG+TGG 0.662854 5_2:-18058372 Msa0761070:CDS
ACTGGCATTGCAAAGCCTGG+TGG 0.663624 5_2:-18058861 Msa0761070:CDS
GTTGCAAATCCAACTCCAGT+GGG 0.671853 5_2:+18059826 None:intergenic
AGGTAACTAAGGTATCTGAA+GGG 0.674479 5_2:-18059280 Msa0761070:CDS
TGTTGTTCTTGTTCACCACG+TGG 0.675934 5_2:+18061814 None:intergenic
CACCTGGAGTCTGAGAAGGG+GGG 0.678451 5_2:+18058733 None:intergenic
AGGACTGTTTCATCTGATAG+CGG 0.680625 5_2:+18060617 None:intergenic
AATTATCCACAAATACCACA+GGG 0.684146 5_2:+18060569 None:intergenic
TCCGCAAAACTTGAAAACCA+AGG 0.687758 5_2:+18059206 None:intergenic
TCATCAGGAGCATAATCCGG+TGG 0.690832 5_2:+18061478 None:intergenic
TTTATGTCCGTGATGCACAC+CGG 0.691794 5_2:-18061628 Msa0761070:CDS
GATGCTAAGATTAAATGTGA+GGG 0.695012 5_2:-18059978 Msa0761070:CDS
GGTGGATACCAAGCTTCCGG+CGG 0.695616 5_2:+18061496 None:intergenic
TGGAAGAGTTATCTCCCCTG+TGG 0.704661 5_2:-18060584 Msa0761070:CDS
CATTTGAAGACAGAGAAGCA+AGG 0.707732 5_2:-18058602 Msa0761070:CDS
AAATCACCATGATCTCGCTG+CGG 0.730412 5_2:+18061598 None:intergenic
TCTGATTCAAATTCACCACG+TGG 0.785823 5_2:-18061829 Msa0761070:CDS
ATTATCCACAAATACCACAG+GGG 0.791011 5_2:+18060570 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTGATTTAAATGTATA+TGG - chr5_2:18059281-18059300 Msa0761070:CDS 15.0%
!!! TTTTGAAGAAATGAAATTCT+TGG + chr5_2:18058151-18058170 None:intergenic 20.0%
!!! CATTGAAATTTTGAAAGTTA+GGG + chr5_2:18058214-18058233 None:intergenic 20.0%
!!! ACATTGAAATTTTGAAAGTT+AGG + chr5_2:18058215-18058234 None:intergenic 20.0%
!! AATTACTAATACAAAATCAC+AGG + chr5_2:18058992-18059011 None:intergenic 20.0%
!! GCACAAACAATTAATATTAA+AGG + chr5_2:18059068-18059087 None:intergenic 20.0%
!! AGTAGTGAGTATTATATTAT+TGG - chr5_2:18059610-18059629 Msa0761070:CDS 20.0%
!! TAGAACCTTTAATTAATGTA+AGG - chr5_2:18059644-18059663 Msa0761070:CDS 20.0%
!! ACTAACCTTACATTAATTAA+AGG + chr5_2:18059652-18059671 None:intergenic 20.0%
!!! TTTTCATTCAAAATAGTGTA+AGG + chr5_2:18060262-18060281 None:intergenic 20.0%
!! ACATGTTTAATGATAAAAGT+TGG - chr5_2:18060542-18060561 Msa0761070:intron 20.0%
!! TTTATTTAACACGTAATATC+TGG - chr5_2:18060751-18060770 Msa0761070:intron 20.0%
!! AAATAGAAAATGATTTATCC+TGG + chr5_2:18060955-18060974 None:intergenic 20.0%
!! AATGAAAATATTGAAAAGAG+TGG - chr5_2:18060977-18060996 Msa0761070:intron 20.0%
!! ATGAAAATATTGAAAAGAGT+GGG - chr5_2:18060978-18060997 Msa0761070:intron 20.0%
!!! AATTTTGAAAGTTAGGGTTT+CGG + chr5_2:18058208-18058227 None:intergenic 25.0%
! ATGGTGATTTATCAGATATT+TGG - chr5_2:18058478-18058497 Msa0761070:CDS 25.0%
! ATTTAGATCAAATATGTGTC+TGG + chr5_2:18058892-18058911 None:intergenic 25.0%
!! ATTAATATTAAAGGTGTGTC+TGG + chr5_2:18059059-18059078 None:intergenic 25.0%
! ATTAATTGTTTGTGCAACAT+AGG - chr5_2:18059073-18059092 Msa0761070:CDS 25.0%
!!! AAGATTTTGACCTTTAGATT+GGG - chr5_2:18059384-18059403 Msa0761070:intron 25.0%
! TATCCTTAATACCATATTCA+TGG - chr5_2:18059524-18059543 Msa0761070:intron 25.0%
! TTTCCATGAATATGGTATTA+AGG + chr5_2:18059530-18059549 None:intergenic 25.0%
!! TTGCATATCTTTTCAATGAA+AGG - chr5_2:18059555-18059574 Msa0761070:intron 25.0%
! TAGAATTGTCTGTCATTAAT+TGG - chr5_2:18059581-18059600 Msa0761070:CDS 25.0%
!! TTAAAGGTTCTATATTTTGC+AGG + chr5_2:18059636-18059655 None:intergenic 25.0%
!!! AGGTTAGTTTCTTTTACTTT+TGG - chr5_2:18059664-18059683 Msa0761070:CDS 25.0%
!!! TTAGTTTCTTTTACTTTTGG+TGG - chr5_2:18059667-18059686 Msa0761070:CDS 25.0%
! GAAGCTGAAAAATAAATACA+AGG + chr5_2:18059943-18059962 None:intergenic 25.0%
! TCAGTATAAAGAATTCACAA+GGG + chr5_2:18060325-18060344 None:intergenic 25.0%
!!! TTTCTTCAAGATTTTTTCCT+AGG - chr5_2:18060597-18060616 Msa0761070:CDS 25.0%
! AGTTTGCAAAAACAGTAAAA+TGG + chr5_2:18060650-18060669 None:intergenic 25.0%
!! TGTTTTCTTTGCATATAGTT+AGG - chr5_2:18060688-18060707 Msa0761070:intron 25.0%
!! GTTTTCTTTGCATATAGTTA+GGG - chr5_2:18060689-18060708 Msa0761070:intron 25.0%
! GGCTTATTTAATAATTGCAT+TGG - chr5_2:18060710-18060729 Msa0761070:intron 25.0%
! GTAATATCTGGTAATTATTC+AGG - chr5_2:18060763-18060782 Msa0761070:intron 25.0%
! TGAAAATATTGAAAAGAGTG+GGG - chr5_2:18060979-18060998 Msa0761070:intron 25.0%
!! TCAATAACATCATCATTTTC+AGG + chr5_2:18061067-18061086 None:intergenic 25.0%
!!! TGAAGATTTTTCTGATTTGA+TGG + chr5_2:18061124-18061143 None:intergenic 25.0%
! CAGAAAAATCTTCATTTGAA+TGG - chr5_2:18061130-18061149 Msa0761070:intron 25.0%
! AAATCTTCATTTGAATGGAT+TGG - chr5_2:18061135-18061154 Msa0761070:intron 25.0%
!! AAAAAGTATATTCAATCACC+TGG + chr5_2:18061349-18061368 None:intergenic 25.0%
!!! GGTGATTGAATATACTTTTT+TGG - chr5_2:18061349-18061368 Msa0761070:intron 25.0%
!!! TCTTTTTTCTCTTGTTTTCT+GGG + chr5_2:18061613-18061632 None:intergenic 25.0%
!! ATTGAATAGTCTTTGACTTT+AGG - chr5_2:18061729-18061748 Msa0761070:CDS 25.0%
GAAGAAATGAAATTCTTGGT+TGG + chr5_2:18058147-18058166 None:intergenic 30.0%
ATTGAGAGAAAGAGAAGTTT+AGG + chr5_2:18058175-18058194 None:intergenic 30.0%
TGATTTATCAGATATTTGGC+CGG - chr5_2:18058482-18058501 Msa0761070:CDS 30.0%
AAAATCCGAGTAGAATTAAC+TGG + chr5_2:18058540-18058559 None:intergenic 30.0%
! AGTTAATTCTACTCGGATTT+TGG - chr5_2:18058539-18058558 Msa0761070:CDS 30.0%
! GAACTGGTTGTATGATTTTA+AGG - chr5_2:18058611-18058630 Msa0761070:CDS 30.0%
!! TGATTTTAAGGAATCCTTGA+AGG - chr5_2:18058623-18058642 Msa0761070:CDS 30.0%
ATCATAAACTTCATCCTTCA+AGG + chr5_2:18058640-18058659 None:intergenic 30.0%
ATCCAGATTAAATGTGTGTT+TGG + chr5_2:18058967-18058986 None:intergenic 30.0%
!! CAACATAGGTTTTGTCTTTT+TGG - chr5_2:18059087-18059106 Msa0761070:CDS 30.0%
!! TTGACGATCTTTTGTTTAAC+AGG - chr5_2:18059124-18059143 Msa0761070:CDS 30.0%
!! TGACGATCTTTTGTTTAACA+GGG - chr5_2:18059125-18059144 Msa0761070:CDS 30.0%
!! GAAGATTTTGACCTTTAGAT+TGG - chr5_2:18059383-18059402 Msa0761070:intron 30.0%
AATTATCCACAAATACCACA+GGG + chr5_2:18059497-18059516 None:intergenic 30.0%
AAATTATCCACAAATACCAC+AGG + chr5_2:18059498-18059517 None:intergenic 30.0%
CAAGAAAGTTTCCATGAATA+TGG + chr5_2:18059538-18059557 None:intergenic 30.0%
GAAAATTCTGATACAAGTCA+TGG + chr5_2:18059700-18059719 None:intergenic 30.0%
!! TTTTAGAAGACATATGCAAG+AGG - chr5_2:18059726-18059745 Msa0761070:intron 30.0%
!! GACATGTTTTTCGAATTTTG+AGG - chr5_2:18059789-18059808 Msa0761070:intron 30.0%
!! ATGTTTTTCGAATTTTGAGG+AGG - chr5_2:18059792-18059811 Msa0761070:intron 30.0%
AATATTGCGAAGAAAGTCTT+TGG - chr5_2:18059817-18059836 Msa0761070:CDS 30.0%
CATACCATTCTGAATCAAAA+AGG + chr5_2:18059900-18059919 None:intergenic 30.0%
!!! CATTTTCTATTTCTAGACTC+AGG + chr5_2:18060015-18060034 None:intergenic 30.0%
! AGATACTATTTCAAGTGCAT+TGG - chr5_2:18060036-18060055 Msa0761070:CDS 30.0%
TGATGCTAAGATTAAATGTG+AGG - chr5_2:18060084-18060103 Msa0761070:CDS 30.0%
GATGCTAAGATTAAATGTGA+GGG - chr5_2:18060085-18060104 Msa0761070:CDS 30.0%
CTGTCTTAAACCTCTTTAAA+TGG + chr5_2:18060177-18060196 None:intergenic 30.0%
GTCAGTATAAAGAATTCACA+AGG + chr5_2:18060326-18060345 None:intergenic 30.0%
TGCCTGTCAACATAAAAAAT+GGG + chr5_2:18060348-18060367 None:intergenic 30.0%
ATGCCTGTCAACATAAAAAA+TGG + chr5_2:18060349-18060368 None:intergenic 30.0%
AAAAGTTGGCTGTAACTTTA+TGG - chr5_2:18060556-18060575 Msa0761070:intron 30.0%
GGTAATTATTCAGGTAACTA+AGG - chr5_2:18060772-18060791 Msa0761070:intron 30.0%
! ATGATGTCTAAAACAGACTT+GGG + chr5_2:18060926-18060945 None:intergenic 30.0%
! AATGATGTCTAAAACAGACT+TGG + chr5_2:18060927-18060946 None:intergenic 30.0%
!! TCTGTTTTAGACATCATTGA+TGG - chr5_2:18060929-18060948 Msa0761070:intron 30.0%
AGACTTCTCAGAAAATTCTT+TGG + chr5_2:18061014-18061033 None:intergenic 30.0%
!!! AGACTGCATTTGTTTTTATC+AGG + chr5_2:18061253-18061272 None:intergenic 30.0%
TGATAAAAACAAATGCAGTC+TGG - chr5_2:18061252-18061271 Msa0761070:intron 30.0%
TTGTTCAATAACCATTGAGA+TGG - chr5_2:18061403-18061422 Msa0761070:CDS 30.0%
!!! CTTTTTTCTCTTGTTTTCTG+GGG + chr5_2:18061612-18061631 None:intergenic 30.0%
!!! GTCTTTTTTCTCTTGTTTTC+TGG + chr5_2:18061614-18061633 None:intergenic 30.0%
AACAAAAAGACCACAGATAA+AGG + chr5_2:18061792-18061811 None:intergenic 30.0%
TGTCATGACTACATACAATT+TGG + chr5_2:18061832-18061851 None:intergenic 30.0%
ACAGATACAAATAGAAATCG+TGG - chr5_2:18061881-18061900 Msa0761070:five_prime_UTR 30.0%
AAAGAAATACCCAATTGAGC+AGG + chr5_2:18058330-18058349 None:intergenic 35.0%
TGCTCAATTGGGTATTTCTT+TGG - chr5_2:18058329-18058348 Msa0761070:three_prime_UTR 35.0%
GACGTTTATGTTGATGTTGT+CGG - chr5_2:18058372-18058391 Msa0761070:CDS 35.0%
GATTTATCAGATATTTGGCC+GGG - chr5_2:18058483-18058502 Msa0761070:CDS 35.0%
ATTTATCAGATATTTGGCCG+GGG - chr5_2:18058484-18058503 Msa0761070:CDS 35.0%
AAAACGCTTACAATCGTCAA+TGG + chr5_2:18058681-18058700 None:intergenic 35.0%
TCTACATGTAAAAGTCGTGT+CGG + chr5_2:18058771-18058790 None:intergenic 35.0%
AATTCAGATCAATTGTGCGT+CGG + chr5_2:18058831-18058850 None:intergenic 35.0%
CTGAATTGTATGTGCTACAT+AGG - chr5_2:18058844-18058863 Msa0761070:CDS 35.0%
CACCAAACACACATTTAATC+TGG - chr5_2:18058962-18058981 Msa0761070:CDS 35.0%
ACCTTTAGATTGGGAATTTC+AGG - chr5_2:18059393-18059412 Msa0761070:intron 35.0%
ACCTGAAATTCCCAATCTAA+AGG + chr5_2:18059397-18059416 None:intergenic 35.0%
! TTGGGAATTTCAGGTCATAA+TGG - chr5_2:18059402-18059421 Msa0761070:intron 35.0%
! TGGGAATTTCAGGTCATAAT+GGG - chr5_2:18059403-18059422 Msa0761070:intron 35.0%
ATTATCCACAAATACCACAG+GGG + chr5_2:18059496-18059515 None:intergenic 35.0%
!! GTCTCCTTTTTGATTCAGAA+TGG - chr5_2:18059893-18059912 Msa0761070:CDS 35.0%
AAATGGAATGCAGCAATTGA+AGG + chr5_2:18060160-18060179 None:intergenic 35.0%
TTGCTGCATTCCATTTAAAG+AGG - chr5_2:18060164-18060183 Msa0761070:intron 35.0%
!!! GACCCATTTTTTATGTTGAC+AGG - chr5_2:18060343-18060362 Msa0761070:CDS 35.0%
TATGCAGTTGTTGTGCATAA+TGG - chr5_2:18060386-18060405 Msa0761070:intron 35.0%
! GCATAAGTTGGATGATTCAA+TGG - chr5_2:18060466-18060485 Msa0761070:intron 35.0%
TCTGCTATACTTGTTCTACT+TGG - chr5_2:18060494-18060513 Msa0761070:intron 35.0%
AGTTTCAAGCTATGAAGACT+GGG + chr5_2:18060625-18060644 None:intergenic 35.0%
TAGTTTCAAGCTATGAAGAC+TGG + chr5_2:18060626-18060645 None:intergenic 35.0%
! AGGTAACTAAGGTATCTGAA+GGG - chr5_2:18060783-18060802 Msa0761070:intron 35.0%
!! ATATTGTTTTATGCCCGACA+AGG - chr5_2:18060830-18060849 Msa0761070:CDS 35.0%
!!! ACCTTGGTTTTCAAGTTTTG+CGG - chr5_2:18060856-18060875 Msa0761070:CDS 35.0%
ATAATTCACAGGTAGCACTT+GGG + chr5_2:18061173-18061192 None:intergenic 35.0%
AGTGGTAAAAGATCATCATC+AGG - chr5_2:18061488-18061507 Msa0761070:CDS 35.0%
! AAAGACTGATTCATCCAAGT+CGG - chr5_2:18061628-18061647 Msa0761070:CDS 35.0%
! AGTCTTTGACTTTAGGCATT+TGG - chr5_2:18061736-18061755 Msa0761070:CDS 35.0%
GGTGAAAATACCTTTATCTG+TGG - chr5_2:18061779-18061798 Msa0761070:CDS 35.0%
! TACAATTTGGCTAGAGGTAT+TGG + chr5_2:18061819-18061838 None:intergenic 35.0%
ACTACATACAATTTGGCTAG+AGG + chr5_2:18061825-18061844 None:intergenic 35.0%
! TTCTCATGCATAATGGAACA+GGG + chr5_2:18061862-18061881 None:intergenic 35.0%
GTATCTGTTCTCATGCATAA+TGG + chr5_2:18061869-18061888 None:intergenic 35.0%
ACAAATAGAAATCGTGGACA+AGG - chr5_2:18061887-18061906 Msa0761070:five_prime_UTR 35.0%
TCTGATTCAAATTCACCACG+TGG - chr5_2:18058234-18058253 Msa0761070:three_prime_UTR 40.0%
CGAGTAGAATTAACTGGAAC+CGG + chr5_2:18058534-18058553 None:intergenic 40.0%
CGGTTCCAGTTAATTCTACT+CGG - chr5_2:18058532-18058551 Msa0761070:CDS 40.0%
! TCATCATCAGGAGCATAATC+CGG + chr5_2:18058591-18058610 None:intergenic 40.0%
TACAACCAGTTCTCATCATC+AGG + chr5_2:18058603-18058622 None:intergenic 40.0%
ATTCAGATCAATTGTGCGTC+GGG + chr5_2:18058830-18058849 None:intergenic 40.0%
CTAAATTGTCTGTGCTACAG+AGG - chr5_2:18058906-18058925 Msa0761070:CDS 40.0%
!! GTTTGGTGTCTAACATGTGT+CGG + chr5_2:18058950-18058969 None:intergenic 40.0%
!! TCTTTTGTTTAACAGGGAGC+TGG - chr5_2:18059131-18059150 Msa0761070:CDS 40.0%
!! CTTTTGTTTAACAGGGAGCT+GGG - chr5_2:18059132-18059151 Msa0761070:CDS 40.0%
AAAAGAAGACCCTCAACTAG+GGG + chr5_2:18059221-18059240 None:intergenic 40.0%
CAAAAGAAGACCCTCAACTA+GGG + chr5_2:18059222-18059241 None:intergenic 40.0%
TCAAAAGAAGACCCTCAACT+AGG + chr5_2:18059223-18059242 None:intergenic 40.0%
ACTCACCATCATTAGGAGAA+TGG + chr5_2:18059250-18059269 None:intergenic 40.0%
! TCCTAATGATGGTGAGTAAG+AGG - chr5_2:18059253-18059272 Msa0761070:CDS 40.0%
TCCTCTTACTCACCATCATT+AGG + chr5_2:18059257-18059276 None:intergenic 40.0%
AGGACTGTTTCATCTGATAG+CGG + chr5_2:18059449-18059468 None:intergenic 40.0%
TGAGTTTATGCAGTGTGCAT+TGG - chr5_2:18059756-18059775 Msa0761070:intron 40.0%
ATTGCGAAGAAAGTCTTTGG+AGG - chr5_2:18059820-18059839 Msa0761070:CDS 40.0%
TCTAGACTCAGGTCAATCAA+TGG + chr5_2:18060004-18060023 None:intergenic 40.0%
TGCATTGGAGTCTTTCACAA+TGG - chr5_2:18060051-18060070 Msa0761070:CDS 40.0%
ATTGGAGTCTTTCACAATGG+TGG - chr5_2:18060054-18060073 Msa0761070:CDS 40.0%
!! CTGCAATGAAGAAGTGTCTA+TGG - chr5_2:18060108-18060127 Msa0761070:intron 40.0%
TATGGAGAAGCAGCTTATGT+TGG - chr5_2:18060126-18060145 Msa0761070:intron 40.0%
CAAGCATATCAATTTCCCAC+TGG - chr5_2:18060222-18060241 Msa0761070:intron 40.0%
TATCAATTTCCCACTGGAGT+TGG - chr5_2:18060228-18060247 Msa0761070:CDS 40.0%
AATGGAACTTCATCTGACTC+TGG - chr5_2:18060404-18060423 Msa0761070:intron 40.0%
ATGGAACTTCATCTGACTCT+GGG - chr5_2:18060405-18060424 Msa0761070:intron 40.0%
! CAGGTAACTAAGGTATCTGA+AGG - chr5_2:18060782-18060801 Msa0761070:intron 40.0%
TCCGCAAAACTTGAAAACCA+AGG + chr5_2:18060860-18060879 None:intergenic 40.0%
TTCCATGCTTGAATCTGAGT+AGG - chr5_2:18060888-18060907 Msa0761070:CDS 40.0%
ATCCTACTCAGATTCAAGCA+TGG + chr5_2:18060893-18060912 None:intergenic 40.0%
! TTTAGACATCATTGATGGCC+AGG - chr5_2:18060934-18060953 Msa0761070:intron 40.0%
AATATTGAAAAGAGTGGGGC+TGG - chr5_2:18060983-18061002 Msa0761070:intron 40.0%
CATAATTCACAGGTAGCACT+TGG + chr5_2:18061174-18061193 None:intergenic 40.0%
! ACCATTGAGATGGATTTGTC+AGG - chr5_2:18061413-18061432 Msa0761070:CDS 40.0%
ACCTGACAAATCCATCTCAA+TGG + chr5_2:18061417-18061436 None:intergenic 40.0%
CATTTGAAGACAGAGAAGCA+AGG - chr5_2:18061461-18061480 Msa0761070:CDS 40.0%
ATCATCATCAGGCAAGTCTT+TGG - chr5_2:18061499-18061518 Msa0761070:CDS 40.0%
ATCCTCAAAATAAAGCTGCC+AGG - chr5_2:18061525-18061544 Msa0761070:CDS 40.0%
TCCTCAAAATAAAGCTGCCA+GGG - chr5_2:18061526-18061545 Msa0761070:CDS 40.0%
CATATTCCTAACATATGCCC+TGG + chr5_2:18061546-18061565 None:intergenic 40.0%
GCATATGTTAGGAATATGCC+TGG - chr5_2:18061548-18061567 Msa0761070:CDS 40.0%
TTAGGAATATGCCTGGTTCA+AGG - chr5_2:18061555-18061574 Msa0761070:CDS 40.0%
!! TTCAAGGAGAGGTGCATTTT+TGG - chr5_2:18061571-18061590 Msa0761070:CDS 40.0%
!! GGTGCATTTTTGGACTTACT+TGG - chr5_2:18061581-18061600 Msa0761070:CDS 40.0%
!! GCATTTTTGGACTTACTTGG+TGG - chr5_2:18061584-18061603 Msa0761070:CDS 40.0%
!!! TGGCTGTGTACTTGGTTTTT+TGG - chr5_2:18061756-18061775 Msa0761070:CDS 40.0%
!!! GGCTGTGTACTTGGTTTTTT+GGG - chr5_2:18061757-18061776 Msa0761070:CDS 40.0%
!!! GCTGTGTACTTGGTTTTTTG+GGG - chr5_2:18061758-18061777 Msa0761070:CDS 40.0%
! GTTCTCATGCATAATGGAAC+AGG + chr5_2:18061863-18061882 None:intergenic 40.0%
TGTTGTTCTTGTTCACCACG+TGG + chr5_2:18058252-18058271 None:intergenic 45.0%
AACCGTAGATCCTGCTCAAT+TGG - chr5_2:18058317-18058336 Msa0761070:three_prime_UTR 45.0%
ACCGTAGATCCTGCTCAATT+GGG - chr5_2:18058318-18058337 Msa0761070:three_prime_UTR 45.0%
ACCCAATTGAGCAGGATCTA+CGG + chr5_2:18058322-18058341 None:intergenic 45.0%
AGTTTCTGAGCCTGAGGATA+TGG - chr5_2:18058395-18058414 Msa0761070:CDS 45.0%
GTTTCTGAGCCTGAGGATAT+GGG - chr5_2:18058396-18058415 Msa0761070:CDS 45.0%
TTTCTGAGCCTGAGGATATG+GGG - chr5_2:18058397-18058416 Msa0761070:CDS 45.0%
TTGGTTAACCCCATATCCTC+AGG + chr5_2:18058408-18058427 None:intergenic 45.0%
CACGGACATAAACTCATCGT+TGG + chr5_2:18058427-18058446 None:intergenic 45.0%
TTTATGTCCGTGATGCACAC+CGG - chr5_2:18058435-18058454 Msa0761070:CDS 45.0%
AAATCACCATGATCTCGCTG+CGG + chr5_2:18058468-18058487 None:intergenic 45.0%
GCATACACAGCAAGATCCAT+TGG + chr5_2:18058510-18058529 None:intergenic 45.0%
! ATGCTCCTGATGATGAGAAC+TGG - chr5_2:18058595-18058614 Msa0761070:CDS 45.0%
AAAGCATGTGTTGCCAAGGT+TGG + chr5_2:18059162-18059181 None:intergenic 45.0%
! TGAGAAAGCATGTGTTGCCA+AGG + chr5_2:18059166-18059185 None:intergenic 45.0%
AGCAGCCATTCTCCTAATGA+TGG - chr5_2:18059242-18059261 Msa0761070:CDS 45.0%
AAACAGTCCTTGCAGTCTAC+TGG - chr5_2:18059459-18059478 Msa0761070:intron 45.0%
AACTCTTCCAGTAGACTGCA+AGG + chr5_2:18059469-18059488 None:intergenic 45.0%
TATCTCCCCTGTGGTATTTG+TGG - chr5_2:18059488-18059507 Msa0761070:intron 45.0%
! GAAAGTCTTTGGAGGCAGTT+TGG - chr5_2:18059828-18059847 Msa0761070:CDS 45.0%
GCTCATTAGTGTCAGAAGAG+TGG + chr5_2:18059982-18060001 None:intergenic 45.0%
GTTGCAAATCCAACTCCAGT+GGG + chr5_2:18060240-18060259 None:intergenic 45.0%
TCCAGACACATGGCATAAGT+TGG - chr5_2:18060454-18060473 Msa0761070:intron 45.0%
TCCAACTTATGCCATGTGTC+TGG + chr5_2:18060458-18060477 None:intergenic 45.0%
AAAACCAAGGTGTGCCTTGT+CGG + chr5_2:18060847-18060866 None:intergenic 45.0%
AATCACCTGGAGTCTGAGAA+GGG + chr5_2:18061336-18061355 None:intergenic 45.0%
TCAAATGATCGCGTGAGATC+TGG + chr5_2:18061448-18061467 None:intergenic 45.0%
ACAGAGAAGCAAGGAAGTAG+TGG - chr5_2:18061470-18061489 Msa0761070:CDS 45.0%
AATATGCCTGGTTCAAGGAG+AGG - chr5_2:18061560-18061579 Msa0761070:CDS 45.0%
TAGGCATTTGGCTGTGTACT+TGG - chr5_2:18061748-18061767 Msa0761070:CDS 45.0%
AACATCACCGGTGTGCATCA+CGG + chr5_2:18058445-18058464 None:intergenic 50.0%
! GGATCTTGCTGTGTATGCTC+CGG - chr5_2:18058512-18058531 Msa0761070:CDS 50.0%
! TCATCAGGAGCATAATCCGG+TGG + chr5_2:18058588-18058607 None:intergenic 50.0%
TGGTGTCTAACATGTGTCGG+TGG + chr5_2:18058947-18058966 None:intergenic 50.0%
CATACTGTGCCCCTAGTTGA+GGG - chr5_2:18059209-18059228 Msa0761070:CDS 50.0%
TGGAAGAGTTATCTCCCCTG+TGG - chr5_2:18059479-18059498 Msa0761070:intron 50.0%
TGGAGGCAGTTTGGTTAGCA+AGG - chr5_2:18059837-18059856 Msa0761070:CDS 50.0%
GGTTGCAAATCCAACTCCAG+TGG + chr5_2:18060241-18060260 None:intergenic 50.0%
AAACCAAGGTGTGCCTTGTC+GGG + chr5_2:18060846-18060865 None:intergenic 50.0%
! TTCTGATTTGATGGCCCAGC+AGG + chr5_2:18061115-18061134 None:intergenic 50.0%
TCAGCATATGAACTCCCACC+AGG + chr5_2:18061220-18061239 None:intergenic 50.0%
ATCACCTGGAGTCTGAGAAG+GGG + chr5_2:18061335-18061354 None:intergenic 50.0%
CAATCACCTGGAGTCTGAGA+AGG + chr5_2:18061337-18061356 None:intergenic 50.0%
!!! GCCCTGGCAGCTTTATTTTG+AGG + chr5_2:18061530-18061549 None:intergenic 50.0%
AAGCTGCCAGGGCATATGTT+AGG - chr5_2:18061537-18061556 Msa0761070:CDS 50.0%
AATGCACCTCTCCTTGAACC+AGG + chr5_2:18061569-18061588 None:intergenic 50.0%
AGCAGTGCTCGCAAGAAATC+AGG - chr5_2:18061662-18061681 Msa0761070:CDS 50.0%
AAATCAGGCCATGCTTCCGT+CGG - chr5_2:18061677-18061696 Msa0761070:CDS 50.0%
TGTCGGAGTTTCTGAGCCTG+AGG - chr5_2:18058389-18058408 Msa0761070:CDS 55.0%
ATCTCGCTGCGGAACATCAC+CGG + chr5_2:18058457-18058476 None:intergenic 55.0%
GATGTTCCGCAGCGAGATCA+TGG - chr5_2:18058459-18058478 Msa0761070:CDS 55.0%
AGATATTTGGCCGGGGCCAA+TGG - chr5_2:18058491-18058510 Msa0761070:CDS 55.0%
ACAGCAAGATCCATTGGCCC+CGG + chr5_2:18058504-18058523 None:intergenic 55.0%
TCCGGTGGATACCAAGCTTC+CGG + chr5_2:18058573-18058592 None:intergenic 55.0%
GCATACTGTGCCCCTAGTTG+AGG - chr5_2:18059208-18059227 Msa0761070:CDS 55.0%
TTCGCACTGCTCCAGACACA+TGG - chr5_2:18060444-18060463 Msa0761070:intron 55.0%
GCTATGAAGACTGGGCTCCT+AGG + chr5_2:18060617-18060636 None:intergenic 55.0%
TGAAGGGACTGTGCTGTCTC+AGG - chr5_2:18060799-18060818 Msa0761070:intron 55.0%
! GCAGGAACTTGTTTGCGGCA+AGG + chr5_2:18061097-18061116 None:intergenic 55.0%
TGCCGCAAACAAGTTCCTGC+TGG - chr5_2:18061097-18061116 Msa0761070:intron 55.0%
GCCGCAAACAAGTTCCTGCT+GGG - chr5_2:18061098-18061117 Msa0761070:intron 55.0%
! GCCCAGCAGGAACTTGTTTG+CGG + chr5_2:18061102-18061121 None:intergenic 55.0%
!! GTCGGGCTGGCATAATTCAC+AGG + chr5_2:18061184-18061203 None:intergenic 55.0%
GTGAATTATGCCAGCCCGAC+TGG - chr5_2:18061184-18061203 Msa0761070:intron 55.0%
CAGGCTTTGCAATGCCAGTC+GGG + chr5_2:18061201-18061220 None:intergenic 55.0%
CCAGGCTTTGCAATGCCAGT+CGG + chr5_2:18061202-18061221 None:intergenic 55.0%
ACTGGCATTGCAAAGCCTGG+TGG - chr5_2:18061202-18061221 Msa0761070:intron 55.0%
CTGGCATTGCAAAGCCTGGT+GGG - chr5_2:18061203-18061222 Msa0761070:intron 55.0%
TCACCTGGAGTCTGAGAAGG+GGG + chr5_2:18061334-18061353 None:intergenic 55.0%
GCTGCTGTCTTTGTCCGACT+TGG + chr5_2:18061645-18061664 None:intergenic 55.0%
GTGAAACTCCAGCTGCCACA+GGG + chr5_2:18061709-18061728 None:intergenic 55.0%
TGTGAAACTCCAGCTGCCAC+AGG + chr5_2:18061710-18061729 None:intergenic 55.0%
!!! TCGGATTTTGGCTGTGCCGC+CGG - chr5_2:18058551-18058570 Msa0761070:CDS 60.0%
GGTGGATACCAAGCTTCCGG+CGG + chr5_2:18058570-18058589 None:intergenic 60.0%
GCCGGAAGCTTGGTATCCAC+CGG - chr5_2:18058569-18058588 Msa0761070:CDS 60.0%
ATGCCCGACAAGGCACACCT+TGG - chr5_2:18060840-18060859 Msa0761070:CDS 60.0%
CTTTGCAATGCCAGTCGGGC+TGG + chr5_2:18061197-18061216 None:intergenic 60.0%
CCGACTGGCATTGCAAAGCC+TGG - chr5_2:18061199-18061218 Msa0761070:intron 60.0%
GGGGGAGTTGGGAGGAGTTA+AGG + chr5_2:18061314-18061333 None:intergenic 60.0%
GTCTGAGAAGGGGGGGAGTT+GGG + chr5_2:18061325-18061344 None:intergenic 60.0%
AGTCTGAGAAGGGGGGGAGT+TGG + chr5_2:18061326-18061345 None:intergenic 60.0%
ACCTGGAGTCTGAGAAGGGG+GGG + chr5_2:18061332-18061351 None:intergenic 60.0%
CACCTGGAGTCTGAGAAGGG+GGG + chr5_2:18061333-18061352 None:intergenic 60.0%
GGAGCTGGGCTTGCCAACCT+TGG - chr5_2:18059146-18059165 Msa0761070:CDS 65.0%
TGAGAAGGGGGGGAGTTGGG+AGG + chr5_2:18061322-18061341 None:intergenic 65.0%
TCCCCCCCTTCTCAGACTCC+AGG - chr5_2:18061328-18061347 Msa0761070:intron 65.0%
GGTAGCTGCCGACGGAAGCA+TGG + chr5_2:18061688-18061707 None:intergenic 65.0%
TTCCGTCGGCAGCTACCCTG+TGG - chr5_2:18061691-18061710 Msa0761070:CDS 65.0%
TGCCACAGGGTAGCTGCCGA+CGG + chr5_2:18061696-18061715 None:intergenic 65.0%
TGGCTGTGCCGCCGGAAGCT+TGG - chr5_2:18058559-18058578 Msa0761070:CDS 70.0%
GGCAGCTACCCTGTGGCAGC+TGG - chr5_2:18061698-18061717 Msa0761070:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 18058137 18061948 18058137 ID=Msa0761070;Name=Msa0761070
chr5_2 mRNA 18058137 18061948 18058137 ID=Msa0761070-mRNA-1;Parent=Msa0761070;Name=Msa0761070-mRNA-1;_AED=0.20;_eAED=0.20;_QI=94|0.85|0.87|1|1|1|8|215|721
chr5_2 exon 18058137 18058650 18058137 ID=Msa0761070-mRNA-1:exon:7842;Parent=Msa0761070-mRNA-1
chr5_2 exon 18058736 18059300 18058736 ID=Msa0761070-mRNA-1:exon:7841;Parent=Msa0761070-mRNA-1
chr5_2 exon 18059580 18059720 18059580 ID=Msa0761070-mRNA-1:exon:7840;Parent=Msa0761070-mRNA-1
chr5_2 exon 18059797 18060126 18059797 ID=Msa0761070-mRNA-1:exon:7839;Parent=Msa0761070-mRNA-1
chr5_2 exon 18060227 18060372 18060227 ID=Msa0761070-mRNA-1:exon:7838;Parent=Msa0761070-mRNA-1
chr5_2 exon 18060563 18060670 18060563 ID=Msa0761070-mRNA-1:exon:7837;Parent=Msa0761070-mRNA-1
chr5_2 exon 18060822 18060939 18060822 ID=Msa0761070-mRNA-1:exon:7836;Parent=Msa0761070-mRNA-1
chr5_2 exon 18061396 18061948 18061396 ID=Msa0761070-mRNA-1:exon:7835;Parent=Msa0761070-mRNA-1
chr5_2 five_prime_UTR 18061855 18061948 18061855 ID=Msa0761070-mRNA-1:five_prime_utr;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18061396 18061854 18061396 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18060822 18060939 18060822 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18060563 18060670 18060563 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18060227 18060372 18060227 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18059797 18060126 18059797 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18059580 18059720 18059580 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18058736 18059300 18058736 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 CDS 18058352 18058650 18058352 ID=Msa0761070-mRNA-1:cds;Parent=Msa0761070-mRNA-1
chr5_2 three_prime_UTR 18058137 18058351 18058137 ID=Msa0761070-mRNA-1:three_prime_utr;Parent=Msa0761070-mRNA-1
Gene Sequence

>Msa0761070

ATGTCTGATTCAAATTCACCACGTGGTGAACAAGAACAACAAGAGAATCAATTGAATTCATCTTCAACGATGAGTTCAATTTCTGAAACCGTAGATCCTGCTCAATTGGGTATTTCTTTGGCTGATCTTGATGATGACATTGACGTTTATGTTGATGTTGTCGGAGTTTCTGAGCCTGAGGATATGGGGTTAACCAACGATGAGTTTATGTCCGTGATGCACACCGGTGATGTTCCGCAGCGAGATCATGGTGATTTATCAGATATTTGGCCGGGGCCAATGGATCTTGCTGTGTATGCTCCGGTTCCAGTTAATTCTACTCGGATTTTGGCTGTGCCGCCGGAAGCTTGGTATCCACCGGATTATGCTCCTGATGATGAGAACTGGTTGTATGATTTTAAGGAATCCTTGAAGGATGAAGTTTATGATTCTGATTCTAAAGTTGTGCCATTGACGATTGGAGCTGGGCTTGCCAACCTTGGCAACACATGCTTTCTCAATTCAATTCTGCAATGCTTCACGCATACTGTGCCCCTAGTTGAGGGTCTTCTTTTGAGCAGCCATTCTCCTAATGATGGTCATAATGGGTTCTGTGTTATCTGTGCTTTCCGCTATCAGATGAAACAGTCCTTGCAGTCTACTGGAAGAGTTATCTCCCCTGTGGTATTTGTGGATAATTTGAAGCAATTTTCTAATGATTTTAGAAGACATATGCAAGAGGATGCACATGAGTTTATGCAGTGTGCATTGGATAAACTTGAGACATGTTTTTCGAATTTTGAGGAGGATAATATTGCGAAGAAAGTCTTTGGAGGCAGTTTGGTTAGCAAGCTTCAGTGTTGTAACTGTAGCCACTCTTCTGACACTAATGAGCCATTGATTGACCTGAGTCTAGAAATAGAAAATGTAGATACTATTTCAAGTGCATTGGAGTCTTTCACAATGGTGGAAAAGATTGATGCTAAGATTAAATGTGAGGGCTGCAATGAAGAAGTGTCTATGGAGAAGCAGCTTATGTTGGATCAGACACCTTCAATTGCTGCATTCCATTTAAAGAGGTTTAAGACAGTTGAGAAGTCAGTTGAAAAGATTGACAAGCATATCAATTTCCCACTGGAGTTGGATTTGCAACCTTACACTATTTTGAATGAAAACAATGATGCATCATTGAAATATGATCTATATGCAGTTGTTGTGCATAATGGAACTTCATCTGACTCTGGGCATTATTTCTGCTTTGTTCGCACTGCTCCAGACACATGGCATAAGTTGGATGATTCAATGGTAGCATCTGCTATACTTGTAACTAAGGTATCTGAAGGGACTGTGCTGTCTCAGGAAGCATACATATTGTTTTATGCCCGACAAGGCACACCTTGGTTTTCAAGTTTTGCGGAATCTACAATTCCATGCTTGAATCTGAGTAGGATGAACACGTCTCCCAAGTCTGTTTTAGACATCATTGATGGCCAGGATAAATCATTTTCTATTTTAAATGAAAATATTGAAAAGAGTGGGGCTGGTAAATCCAAAGAATTTTCTGAGAAGTCTGATTATTCTTGTCAACAAAGTCGCAAGTTTCCTGAAAATGATGATGTTATTGATGCTTCTCCTTGCCGCAAACAAGTTCCTGCTGGGCCATCAAATCAGAAAAATCTTCATTTGAATGGATTGGAAGATATCAGCGCCCAAGTGCTACCTGTGAATTATGCCAGCCCGACTGGCATTGCAAAGCCTGGTGGGAGTTCATATGCTGAGAATGTTGCTCCTGATAAAAACAAATGCAGTCTGGAAGCTAATGATTTTATAGAGAATGATGTTTTCAATGCCTTAACTCCTCCCAACTCCCCCCCTTCTCAGACTCCAGGTAAGAGTTTCCAGATCTCACGCGATCATTTGAAGACAGAGAAGCAAGGAAGTAGTGGTAAAAGATCATCATCAGGCAAGTCTTTGGATAATCCTCAAAATAAAGCTGCCAGGGCATATGTTAGGAATATGCCTGGTTCAAGGAGAGGTGCATTTTTGGACTTACTTGGTGGTTCCCCAGAAAACAAGAGAAAAAAGACTGATTCATCCAAGTCGGACAAAGACAGCAGCAGTGCTCGCAAGAAATCAGGCCATGCTTCCGTCGGCAGCTACCCTGTGGCAGCTGGAGTTTCACATTGA
Protein sequence

>Msa0761070

MSDSNSPRGEQEQQENQLNSSSTMSSISETVDPAQLGISLADLDDDIDVYVDVVGVSEPEDMGLTNDEFMSVMHTGDVPQRDHGDLSDIWPGPMDLAVYAPVPVNSTRILAVPPEAWYPPDYAPDDENWLYDFKESLKDEVYDSDSKVVPLTIGAGLANLGNTCFLNSILQCFTHTVPLVEGLLLSSHSPNDGHNGFCVICAFRYQMKQSLQSTGRVISPVVFVDNLKQFSNDFRRHMQEDAHEFMQCALDKLETCFSNFEEDNIAKKVFGGSLVSKLQCCNCSHSSDTNEPLIDLSLEIENVDTISSALESFTMVEKIDAKIKCEGCNEEVSMEKQLMLDQTPSIAAFHLKRFKTVEKSVEKIDKHINFPLELDLQPYTILNENNDASLKYDLYAVVVHNGTSSDSGHYFCFVRTAPDTWHKLDDSMVASAILVTKVSEGTVLSQEAYILFYARQGTPWFSSFAESTIPCLNLSRMNTSPKSVLDIIDGQDKSFSILNENIEKSGAGKSKEFSEKSDYSCQQSRKFPENDDVIDASPCRKQVPAGPSNQKNLHLNGLEDISAQVLPVNYASPTGIAKPGGSSYAENVAPDKNKCSLEANDFIENDVFNALTPPNSPPSQTPGKSFQISRDHLKTEKQGSSGKRSSSGKSLDNPQNKAARAYVRNMPGSRRGAFLDLLGGSPENKRKKTDSSKSDKDSSSARKKSGHASVGSYPVAAGVSH*