AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0803350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0803350 MtrunA17_Chr5g0411271 89.379 725 63 5 1 721 1 715 0.0 1225
Msa0803350 MtrunA17_Chr6g0458431 70.475 569 135 9 146 705 87 631 0.0 739
Msa0803350 MtrunA17_Chr6g0458441 64.542 612 143 9 103 705 110 656 0.0 712
Msa0803350 MtrunA17_Chr6g0458421 67.254 568 131 10 153 712 27 547 0.0 708
Msa0803350 MtrunA17_Chr8g0366721 56.329 316 122 4 152 466 209 509 5.14e-110 345
Msa0803350 MtrunA17_Chr8g0365831 47.516 322 155 5 152 466 161 475 2.66e-90 292
Msa0803350 MtrunA17_Chr4g0074041 42.089 316 160 6 153 455 90 395 5.37e-73 253
Msa0803350 MtrunA17_Chr4g0062561 39.062 320 174 9 141 456 239 541 3.52e-55 204
Msa0803350 MtrunA17_Chr4g0062561 38.356 292 157 8 173 456 577 853 7.42e-47 179
Msa0803350 MtrunA17_Chr4g0062491 35.897 312 175 7 166 463 1 301 4.81e-54 189
Msa0803350 MtrunA17_Chr1g0150541 34.700 317 180 9 155 455 181 486 8.78e-49 184
Msa0803350 MtrunA17_Chr3g0133061 34.286 315 181 9 156 455 585 888 5.18e-47 180
Msa0803350 MtrunA17_Chr2g0312901 32.698 315 188 7 156 455 23 328 6.19e-47 177
Msa0803350 MtrunA17_Chr5g0412381 34.395 314 182 9 156 456 425 727 2.40e-46 177
Msa0803350 MtrunA17_Chr2g0313171 35.443 316 177 9 156 455 465 769 3.41e-46 177
Msa0803350 MtrunA17_Chr5g0427301 33.651 315 182 10 156 455 543 845 3.53e-44 171
Msa0803350 MtrunA17_Chr1g0149991 31.534 352 182 11 156 456 182 525 9.70e-40 154
Msa0803350 MtrunA17_Chr8g0382161 30.259 347 191 8 156 457 24 364 1.66e-34 135
Msa0803350 MtrunA17_Chr3g0123951 30.747 348 180 12 156 453 24 360 1.81e-34 135
Msa0803350 MtrunA17_Chr4g0064671 28.400 250 120 8 258 456 5 246 1.67e-27 112
Msa0803350 MtrunA17_Chr1g0148321 29.010 293 175 9 155 430 107 383 2.60e-23 105
Msa0803350 MtrunA17_Chr4g0020741 26.944 360 192 13 156 455 183 531 2.59e-22 101
Msa0803350 MtrunA17_Chr3g0138791 27.226 393 240 17 60 431 17 384 2.71e-21 99.8
Msa0803350 MtrunA17_Chr5g0439971 26.910 301 197 10 142 431 195 483 3.02e-21 99.4
Msa0803350 MtrunA17_Chr3g0119031 27.333 300 195 11 142 430 190 477 4.14e-21 99.0
Msa0803350 MtrunA17_Chr1g0170261 26.923 286 192 11 155 430 201 479 7.48e-20 95.1
Msa0803350 MtrunA17_Chr2g0322211 33.766 154 90 5 305 455 717 861 3.66e-15 79.7
Msa0803350 MtrunA17_Chr1g0189101 35.443 158 89 6 305 458 765 913 4.93e-15 79.3
Msa0803350 MtrunA17_Chr7g0255591 32.911 158 94 6 305 458 754 903 2.06e-14 77.4
Msa0803350 MtrunA17_Chr3g0121291 35.938 128 73 3 336 455 943 1069 2.31e-14 77.4
Msa0803350 MtrunA17_Chr3g0121291 30.457 197 91 7 136 300 202 384 4.47e-12 69.7
Msa0803350 MtrunA17_Chr7g0267641 33.117 154 92 5 305 455 791 936 4.89e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0803350 AT4G17895.1 52.355 361 154 5 135 486 155 506 2.05e-115 363
Msa0803350 AT5G46740.1 38.552 594 304 15 147 696 155 731 1.41e-103 333
Msa0803350 AT5G46740.2 37.430 537 277 14 202 696 3 522 2.81e-83 274
Msa0803350 AT5G57990.1 42.540 315 159 6 153 455 105 409 2.17e-75 260
Msa0803350 AT4G31670.1 35.016 317 179 9 155 455 168 473 3.60e-48 180
Msa0803350 AT5G65450.1 34.505 313 182 8 156 455 330 632 1.09e-47 180
Msa0803350 AT5G65450.2 34.505 313 182 8 156 455 349 651 1.63e-47 180
Msa0803350 AT2G24640.1 32.603 365 212 11 107 455 133 479 2.98e-47 178
Msa0803350 AT3G14400.1 34.169 319 182 8 156 455 25 334 8.83e-47 177
Msa0803350 AT2G24640.2 34.385 317 181 9 155 455 4 309 9.56e-47 174
Msa0803350 AT1G17110.2 32.464 345 195 11 130 455 422 747 3.60e-43 168
Msa0803350 AT1G17110.3 32.464 345 195 11 130 455 418 743 4.98e-43 168
Msa0803350 AT1G17110.1 32.464 345 195 11 130 455 418 743 4.98e-43 168
Msa0803350 AT4G24560.1 31.646 316 188 10 156 455 543 846 1.04e-39 158
Msa0803350 AT2G22310.1 30.659 349 188 11 156 458 24 364 6.97e-33 130
Msa0803350 AT4G39910.1 29.944 354 187 10 156 457 24 368 2.56e-32 129
Msa0803350 AT5G10790.2 28.571 364 187 14 156 456 49 402 9.15e-29 120
Msa0803350 AT5G10790.1 28.571 364 187 14 156 456 178 531 1.64e-27 118
Msa0803350 AT2G22310.3 29.674 337 183 11 168 458 4 332 1.30e-26 112
Msa0803350 AT2G22310.2 30.634 284 150 8 217 458 49 327 2.84e-26 110
Msa0803350 AT3G11910.3 28.000 300 193 11 142 430 189 476 3.56e-23 106
Msa0803350 AT3G11910.1 28.000 300 193 11 142 430 189 476 3.56e-23 106
Msa0803350 AT3G11910.4 28.000 300 193 11 142 430 188 475 3.95e-23 105
Msa0803350 AT3G11910.2 28.000 300 193 11 142 430 188 475 3.95e-23 105
Msa0803350 AT4G30890.1 25.414 362 199 10 156 455 198 550 6.72e-23 103
Msa0803350 AT4G30890.2 25.414 362 199 10 156 455 198 550 6.72e-23 103
Msa0803350 AT4G30890.3 25.414 362 199 10 156 455 198 550 6.72e-23 103
Msa0803350 AT5G06600.2 27.742 310 201 11 142 440 189 486 1.38e-22 104
Msa0803350 AT5G06600.1 27.742 310 201 11 142 440 190 487 1.44e-22 104
Msa0803350 AT5G06600.3 27.742 310 201 11 142 440 190 487 1.75e-22 103
Msa0803350 AT3G49600.2 26.990 289 185 10 155 430 106 381 1.39e-19 94.7
Msa0803350 AT3G49600.1 26.990 289 185 10 155 430 106 381 1.39e-19 94.7
Msa0803350 AT3G49600.3 26.990 289 185 10 155 430 106 381 1.57e-19 94.4
Msa0803350 AT5G22030.1 34.416 154 90 5 305 455 723 868 2.49e-16 84.0
Msa0803350 AT5G22030.2 34.416 154 90 5 305 455 765 910 3.04e-16 83.6
Msa0803350 AT2G40930.1 33.333 156 92 6 305 456 769 916 4.71e-15 79.7
Msa0803350 AT2G40930.2 33.333 156 92 6 305 456 769 916 4.71e-15 79.7
Msa0803350 AT4G10590.4 37.419 155 84 6 305 455 748 893 5.94e-15 79.3
Msa0803350 AT4G10590.4 28.646 192 104 5 155 313 303 494 5.64e-11 66.6
Msa0803350 AT4G10590.3 37.419 155 84 6 305 455 748 893 5.94e-15 79.3
Msa0803350 AT4G10590.3 28.646 192 104 5 155 313 303 494 5.64e-11 66.6
Msa0803350 AT4G10590.1 37.419 155 84 6 305 455 748 893 5.94e-15 79.3
Msa0803350 AT4G10590.1 28.646 192 104 5 155 313 303 494 5.64e-11 66.6
Msa0803350 AT4G10590.2 37.419 155 84 6 305 455 748 893 5.94e-15 79.3
Msa0803350 AT4G10590.2 28.646 192 104 5 155 313 303 494 5.64e-11 66.6
Msa0803350 AT4G10570.1 36.774 155 85 7 305 455 749 894 2.38e-14 77.4
Msa0803350 AT4G10570.1 28.910 211 115 6 138 313 285 495 3.45e-11 67.4
Msa0803350 AT1G32850.1 35.484 155 87 7 305 455 734 879 5.34e-14 76.3
Msa0803350 AT1G32850.1 26.291 213 124 2 134 313 280 492 6.74e-12 69.7
Msa0803350 AT1G04860.1 33.974 156 86 4 156 298 232 383 2.62e-13 74.3

Find 167 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATAAACTTCATCCTTTA+AGG 0.041402 5_3:+18430905 None:intergenic
CTTAACCAACGATGAGTTTA+TGG 0.181543 5_3:-18431123 Msa0803350:CDS
CTGTCTTAAACCTCTTTAAA+TGG 0.207305 5_3:+18429336 None:intergenic
AGTTAATTCTACTCGGATTT+TGG 0.210552 5_3:-18431003 Msa0803350:CDS
TGCTCAATTGGGTATTTCTT+TGG 0.217652 5_3:-18431213 Msa0803350:CDS
AAACAGTCCTTGCAGTTTAC+TGG 0.245751 5_3:-18430051 Msa0803350:CDS
AGACTTCTCAGAAAATTCTT+TGG 0.249608 5_3:+18428501 None:intergenic
ATTGAGAGAAAGAGAAGTTT+AGG 0.251577 5_3:+18431370 None:intergenic
ATGGTGATTTGTCAGATATT+TGG 0.280681 5_3:-18431064 Msa0803350:CDS
AGTTTCTGAGCCTGAGGATA+TGG 0.283386 5_3:-18431147 Msa0803350:CDS
ATTGAATAGTCTTTGACTTT+AGG 0.290779 5_3:-18427783 Msa0803350:exon
TGTCATGACTACATACAATT+TGG 0.304833 5_3:+18427683 None:intergenic
TACAATTTGGCTAGAGGTAT+TGG 0.311108 5_3:+18427696 None:intergenic
AATATTGCGAAGAAAGTCTT+TGG 0.320644 5_3:-18429693 Msa0803350:CDS
AACCGTAGATCCTGCTCAAT+TGG 0.346549 5_3:-18431225 Msa0803350:CDS
AGTCTTTGACTTTAGGCATT+TGG 0.346739 5_3:-18427776 Msa0803350:three_prime_UTR
TATGATTCTGATTCTAAAGT+TGG 0.348463 5_3:-18430888 Msa0803350:CDS
TAGGCATTTGGCTGTGTACT+TGG 0.354596 5_3:-18427764 Msa0803350:three_prime_UTR
GTATCTGTTCTCATGCATAA+TGG 0.357638 5_3:+18427646 None:intergenic
TTGGGAATTTCAGGTCATAA+TGG 0.374228 5_3:-18430108 Msa0803350:intron
AAAATCCGAGTAGAATTAAC+TGG 0.376969 5_3:+18431005 None:intergenic
GTTCTCATGCATAATGGAAC+AGG 0.386413 5_3:+18427652 None:intergenic
TCAAATGATCGCGTGAGATC+TGG 0.390392 5_3:+18428067 None:intergenic
TGGGAATTTCAGGTCATAAT+GGG 0.391890 5_3:-18430107 Msa0803350:intron
TGCCGCAAACAAGTTCCTGC+TGG 0.392129 5_3:-18428415 Msa0803350:CDS
TATGCAGTTGTTGTGCATAA+TGG 0.393951 5_3:-18429124 Msa0803350:CDS
GGCAGCTACCCTGTGGCAGC+TGG 0.397430 5_3:-18427814 Msa0803350:CDS
AGCAGTGCTCGCAAGAAATC+AGG 0.399732 5_3:-18427850 Msa0803350:CDS
GAAAGTCTTTGGAGGCAGTT+TGG 0.402179 5_3:-18429682 Msa0803350:CDS
CTGCAATGAAGAAGTGTCTA+TGG 0.405794 5_3:-18429402 Msa0803350:CDS
TTCTGATTTGATGGCCCAGC+AGG 0.410077 5_3:+18428400 None:intergenic
TCCGGTGGATACCAAGCTTC+CGG 0.412997 5_3:+18430972 None:intergenic
AAAACCAAGGTGTGCCTTGT+CGG 0.413813 5_3:+18428668 None:intergenic
GTGAATTATGCCAGCCCGAC+TGG 0.419613 5_3:-18428328 Msa0803350:CDS
CGAGTAGAATTAACTGGAAC+CGG 0.424615 5_3:+18431011 None:intergenic
GATTTGTCAGATATTTGGCC+GGG 0.424834 5_3:-18431059 Msa0803350:CDS
TCCAACTTATGCCATGTGTC+TGG 0.425693 5_3:+18429055 None:intergenic
ATGATTCTGATTCTAAAGTT+GGG 0.433621 5_3:-18430887 Msa0803350:CDS
TACAACCAGTTCTCATCATC+AGG 0.433784 5_3:+18430942 None:intergenic
GACGTTTATGTTGATGTTGT+CGG 0.434418 5_3:-18431170 Msa0803350:CDS
TGGCTGTGCCGCCGGAAGCT+TGG 0.438381 5_3:-18430983 Msa0803350:CDS
TCCTCTTACTCACCATCATT+AGG 0.442960 5_3:+18430257 None:intergenic
AAATAGAAAATGATTTATCC+TGG 0.443696 5_3:+18428560 None:intergenic
CTTTGCAATGCCAGTCGGGC+TGG 0.444918 5_3:+18428318 None:intergenic
CGGTTCCAGTTAATTCTACT+CGG 0.445652 5_3:-18431010 Msa0803350:CDS
GCTGCTGTCTTTGTCCGACT+TGG 0.449405 5_3:+18427870 None:intergenic
AAATGGAATGCAGCAATTGA+AGG 0.458769 5_3:+18429353 None:intergenic
CAGGCTTTGCAATGCCAGTC+GGG 0.460520 5_3:+18428314 None:intergenic
AAGCTGCCAGGGCATATGTT+AGG 0.462405 5_3:-18427975 Msa0803350:CDS
GGTAATTATTCAGGTAACTA+AGG 0.467491 5_3:-18428740 Msa0803350:intron
TGAAGGGACTGTGCTGTCTC+AGG 0.467824 5_3:-18428713 Msa0803350:CDS
TATCAATTTCCCACTGGAGT+TGG 0.469397 5_3:-18429282 Msa0803350:CDS
AATGGAACTTCATCTGACTC+TGG 0.470300 5_3:-18429106 Msa0803350:CDS
AAACCAAGGTGTGCCTTGTC+GGG 0.471326 5_3:+18428669 None:intergenic
GTTTCTGAGCCTGAGGATAT+GGG 0.471454 5_3:-18431146 Msa0803350:CDS
CATAATTCACAGGTAGCACT+TGG 0.473153 5_3:+18428341 None:intergenic
ACCGTAGATCCTGCTCAATT+GGG 0.475265 5_3:-18431224 Msa0803350:CDS
AGCTGCCATTCTCCTAATGA+TGG 0.476167 5_3:-18430269 Msa0803350:intron
AATATTGAAAAGAGTGGGGC+TGG 0.480181 5_3:-18428529 Msa0803350:CDS
TTGCTGCATTCCATTTAAAG+AGG 0.480468 5_3:-18429346 Msa0803350:CDS
TCAAAAGAAGACCCTCAACT+AGG 0.481413 5_3:+18430291 None:intergenic
AATGAAAATATTGAAAAGAG+TGG 0.488221 5_3:-18428535 Msa0803350:CDS
ATGAAAATATTGAAAAGAGT+GGG 0.488375 5_3:-18428534 Msa0803350:CDS
TTTAGACATCACTGATGGCC+AGG 0.488436 5_3:-18428578 Msa0803350:CDS
GAAGAAATGAAATTCTTGGT+TGG 0.491050 5_3:+18431398 None:intergenic
ATCTCGCTGCGGAACATCAC+CGG 0.496487 5_3:+18431088 None:intergenic
TCTAGACTTAGGTCAATCAA+TGG 0.498501 5_3:+18429509 None:intergenic
CAAAAGAAGACCCTCAACTA+GGG 0.500648 5_3:+18430292 None:intergenic
GCCCAGCAGGAACTTGTTTG+CGG 0.500874 5_3:+18428413 None:intergenic
GCATACTGTGCCCCTAGTTG+AGG 0.501299 5_3:-18430303 Msa0803350:CDS
AGATACTATTTCAAGTGCAT+TGG 0.502024 5_3:-18429474 Msa0803350:CDS
ATCCTCAAAATAAAGCTGCC+AGG 0.504847 5_3:-18427987 Msa0803350:CDS
GATGTTCCGCAGCGAGATCA+TGG 0.509643 5_3:-18431083 Msa0803350:CDS
GCATAAGTTGGATGATTCAA+TGG 0.511129 5_3:-18429044 Msa0803350:CDS
CCGACTGGCATTGCAAAGCC+TGG 0.513078 5_3:-18428313 Msa0803350:CDS
ATCATCATCAGGCAAGTCTT+CGG 0.514591 5_3:-18428013 Msa0803350:CDS
ATGCTCCTGATGATGAGAAC+TGG 0.515150 5_3:-18430947 Msa0803350:CDS
AGTGGTAAAAGATCATCATC+AGG 0.517890 5_3:-18428024 Msa0803350:CDS
TATCTCCCCTGTGGTATTCG+TGG 0.519071 5_3:-18430022 Msa0803350:CDS
TCATCATCAGGAGCATAATC+CGG 0.519460 5_3:+18430954 None:intergenic
CCAGGCTTTGCAATGCCAGT+CGG 0.519525 5_3:+18428313 None:intergenic
AAAGCATGTGTTACCAAGGT+TGG 0.521069 5_3:+18430352 None:intergenic
TGTGAAACTCCAGCTGCCAC+AGG 0.523773 5_3:+18427805 None:intergenic
ACTCACCATCATTAGGAGAA+TGG 0.528124 5_3:+18430264 None:intergenic
GCCGCAAACAAGTTCCTGCT+GGG 0.528262 5_3:-18428414 Msa0803350:CDS
TGGAGGCAGTTTGGTTAGCA+AGG 0.528538 5_3:-18429673 Msa0803350:intron
TGAGTTTATGCAGTGTGCAT+TGG 0.528922 5_3:-18429754 Msa0803350:CDS
CAATCACCTGGAGTCTGAGA+AGG 0.530275 5_3:+18428178 None:intergenic
TTAGGAATATGCCTGGTTCA+AGG 0.531245 5_3:-18427957 Msa0803350:CDS
CATACTGTGCCCCTAGTTGA+GGG 0.532476 5_3:-18430302 Msa0803350:CDS
GTCGGGCTGGCATAATTCAC+AGG 0.532843 5_3:+18428331 None:intergenic
CTGGCATTGCAAAGCCTGGT+GGG 0.544914 5_3:-18428309 Msa0803350:CDS
AATCACCTGGAGTCTGAGAA+GGG 0.545095 5_3:+18428179 None:intergenic
ACAGCAAGATCCATCGCCCC+CGG 0.546748 5_3:+18431041 None:intergenic
ACCCAATTGAGCAGGATCTA+CGG 0.547215 5_3:+18431223 None:intergenic
GGTAGCTGCCGACGGAAGCA+TGG 0.550788 5_3:+18427827 None:intergenic
TGATTTGTCAGATATTTGGC+CGG 0.551375 5_3:-18431060 Msa0803350:CDS
TTGGTTAAGCCCATATCCTC+AGG 0.551945 5_3:+18431137 None:intergenic
GCCGGAAGCTTGGTATCCAC+CGG 0.552170 5_3:-18430973 Msa0803350:CDS
CAGGTAACTAAGGTATCTGA+AGG 0.552413 5_3:-18428730 Msa0803350:CDS
TATGGAGAAGCAGCTTATGT+TGG 0.552768 5_3:-18429384 Msa0803350:CDS
AGTGATGTCTAAAACAGACT+TGG 0.553424 5_3:+18428588 None:intergenic
AAAACGCTTACAATCGTCAA+TGG 0.553452 5_3:+18430864 None:intergenic
GGAGCTGGGCTTGCCAACCT+TGG 0.555047 5_3:-18430365 Msa0803350:CDS
ATGGAACTTCATCTGACTCT+GGG 0.557189 5_3:-18429105 Msa0803350:CDS
ACAGAGAAGCAAGGAAGTAG+TGG 0.558974 5_3:-18428042 Msa0803350:CDS
ACCTGGAGTCTGAGAAGGGG+GGG 0.559134 5_3:+18428183 None:intergenic
TCCAGACACATGGCATAAGT+TGG 0.561978 5_3:-18429056 Msa0803350:CDS
TGCCACAGGGTAGCTGCCGA+CGG 0.563195 5_3:+18427819 None:intergenic
AAATTATCCACGAATACCAC+AGG 0.565734 5_3:+18430015 None:intergenic
TCAGCATATGAACTCCCACC+AGG 0.567820 5_3:+18428295 None:intergenic
GCAGGAACTTGTTTGCGGCA+AGG 0.568113 5_3:+18428418 None:intergenic
TCACCTGGAGTCTGAGAAGG+GGG 0.569077 5_3:+18428181 None:intergenic
TCCTCAAAATAAAGCTGCCA+GGG 0.569220 5_3:-18427986 Msa0803350:CDS
AAAGAAATACCCAATTGAGC+AGG 0.570044 5_3:+18431215 None:intergenic
ATTTGTCAGATATTTGGCCG+GGG 0.570634 5_3:-18431058 Msa0803350:CDS
ACAAATAGAAATCGCGGACA+AGG 0.573534 5_3:-18427625 Msa0803350:three_prime_UTR
ATTGCGAAGAAAGTCTTTGG+AGG 0.579484 5_3:-18429690 Msa0803350:CDS
CATATTCCTAACATATGCCC+TGG 0.580915 5_3:+18427969 None:intergenic
GCATATGTTAGGAATATGCC+TGG 0.584399 5_3:-18427964 Msa0803350:CDS
CAAGCATATCAATTTCCCAC+TGG 0.585157 5_3:-18429288 Msa0803350:CDS
TTCGCACTGCTCCAGACACA+TGG 0.586738 5_3:-18429066 Msa0803350:CDS
AATGCACTTCTCCTTGAACC+AGG 0.588351 5_3:+18427946 None:intergenic
TGCATTGGAGTCTTTCACAA+TGG 0.593024 5_3:-18429459 Msa0803350:CDS
TGAAAATATTGAAAAGAGTG+GGG 0.594431 5_3:-18428533 Msa0803350:CDS
CACAGCCATAAACTCATCGT+TGG 0.599069 5_3:+18431118 None:intergenic
GGATCTTGCTGTGTATGCTC+CGG 0.600782 5_3:-18431030 Msa0803350:CDS
GTGATGTCTAAAACAGACTT+GGG 0.604740 5_3:+18428589 None:intergenic
ATTGGAGTCTTTCACAATGG+TGG 0.605716 5_3:-18429456 Msa0803350:CDS
ATGCCCGACAAGGCACACCT+TGG 0.606806 5_3:-18428672 Msa0803350:CDS
TGATGCTAAGATTAAATGTG+AGG 0.612633 5_3:-18429426 Msa0803350:CDS
ATCACCTGGAGTCTGAGAAG+GGG 0.616978 5_3:+18428180 None:intergenic
TGAGAAAGCATGTGTTACCA+AGG 0.617381 5_3:+18430348 None:intergenic
GGTGAAAATACCTTTATCTG+TGG 0.622911 5_3:-18427733 Msa0803350:three_prime_UTR
AACTCTTCCAGTAAACTGCA+AGG 0.624220 5_3:+18430044 None:intergenic
AAAGACTGATTCATCCAAGT+CGG 0.626726 5_3:-18427884 Msa0803350:CDS
GCTCATTAGTGTCAGAAGAG+TGG 0.628120 5_3:+18429531 None:intergenic
AAATCAGGCCATGCTTCCGT+CGG 0.629660 5_3:-18427835 Msa0803350:CDS
GTGAAACTCCAGCTGCCACA+GGG 0.630296 5_3:+18427806 None:intergenic
ATCCTACTCAGATTCAAGCA+TGG 0.632686 5_3:+18428622 None:intergenic
TTTGTCAGATATTTGGCCGG+GGG 0.639280 5_3:-18431057 Msa0803350:CDS
GGTTGCAAATCCAACTCCAG+TGG 0.642634 5_3:+18429272 None:intergenic
TTCCATGCTTGAATCTGAGT+AGG 0.644297 5_3:-18428624 Msa0803350:CDS
ATAATTCACAGGTAGCACTT+GGG 0.648379 5_3:+18428342 None:intergenic
AAAAGAAGACCCTCAACTAG+GGG 0.651897 5_3:+18430293 None:intergenic
TGTCGGAGTTTCTGAGCCTG+AGG 0.652877 5_3:-18431153 Msa0803350:CDS
ACTACATACAATTTGGCTAG+AGG 0.656761 5_3:+18427690 None:intergenic
TTCTCATGCATAATGGAACA+GGG 0.661403 5_3:+18427653 None:intergenic
ACAGATACAAATAGAAATCG+CGG 0.661812 5_3:-18427631 Msa0803350:three_prime_UTR
TTCCGTCGGCAGCTACCCTG+TGG 0.662854 5_3:-18427821 Msa0803350:CDS
ACTGGCATTGCAAAGCCTGG+TGG 0.663624 5_3:-18428310 Msa0803350:CDS
GTTGCAAATCCAACTCCAGT+GGG 0.671853 5_3:+18429273 None:intergenic
AGGTAACTAAGGTATCTGAA+GGG 0.674479 5_3:-18428729 Msa0803350:CDS
TGTTGTTCTTGTTCACCACG+TGG 0.675934 5_3:+18431293 None:intergenic
CACCTGGAGTCTGAGAAGGG+GGG 0.678451 5_3:+18428182 None:intergenic
AGGACTGTTTCATCTGATAG+CGG 0.680625 5_3:+18430064 None:intergenic
TCCGCAAAACTTGAAAACCA+AGG 0.687758 5_3:+18428655 None:intergenic
TCATCAGGAGCATAATCCGG+TGG 0.690832 5_3:+18430957 None:intergenic
GATGCTAAGATTAAATGTGA+GGG 0.695012 5_3:-18429425 Msa0803350:CDS
GGTGGATACCAAGCTTCCGG+CGG 0.695616 5_3:+18430975 None:intergenic
TTTATGGCTGTGATGTACAC+CGG 0.698846 5_3:-18431107 Msa0803350:CDS
TGGAAGAGTTATCTCCCCTG+TGG 0.704661 5_3:-18430031 Msa0803350:CDS
AATTATCCACGAATACCACA+GGG 0.705529 5_3:+18430016 None:intergenic
CATTTGAAGACAGAGAAGCA+AGG 0.707732 5_3:-18428051 Msa0803350:CDS
AAATCACCATGATCTCGCTG+CGG 0.737924 5_3:+18431077 None:intergenic
TCTGATTCAAATTCACCACG+TGG 0.785823 5_3:-18431308 Msa0803350:CDS
ATTATCCACGAATACCACAG+GGG 0.824419 5_3:+18430017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTGATTTAAATGTATA+TGG - chr5_3:18428761-18428780 Msa0803350:intron 15.0%
!! TAGAACCTTTAATTAATATA+AGG - chr5_3:18429125-18429144 Msa0803350:CDS 15.0%
!! ACTAACCTTATATTAATTAA+AGG + chr5_3:18429133-18429152 None:intergenic 15.0%
!!! TTTTGAAGAAATGAAATTCT+TGG + chr5_3:18427600-18427619 None:intergenic 20.0%
!!! CATTGAAATTTTGAAAGTTA+GGG + chr5_3:18427663-18427682 None:intergenic 20.0%
!!! ACATTGAAATTTTGAAAGTT+AGG + chr5_3:18427664-18427683 None:intergenic 20.0%
!! GCACAAACAATTAATATTAA+AGG + chr5_3:18428549-18428568 None:intergenic 20.0%
!!! TTTTCATTCAAAATAGTGTA+AGG + chr5_3:18429743-18429762 None:intergenic 20.0%
!! ACATGTTTAATGATAAAAGT+TGG - chr5_3:18430023-18430042 Msa0803350:CDS 20.0%
!! TTTATTTAACACGTAATATC+TGG - chr5_3:18430230-18430249 Msa0803350:intron 20.0%
!! AAATAGAAAATGATTTATCC+TGG + chr5_3:18430434-18430453 None:intergenic 20.0%
!! AATGAAAATATTGAAAAGAG+TGG - chr5_3:18430456-18430475 Msa0803350:intron 20.0%
!! ATGAAAATATTGAAAAGAGT+GGG - chr5_3:18430457-18430476 Msa0803350:intron 20.0%
!!! AATTTTGAAAGTTAGGGTTT+CGG + chr5_3:18427657-18427676 None:intergenic 25.0%
!!! TGATTTTAAGGAATCCTTAA+AGG - chr5_3:18428072-18428091 Msa0803350:CDS 25.0%
! ATCATAAACTTCATCCTTTA+AGG + chr5_3:18428089-18428108 None:intergenic 25.0%
!!! TATGATTCTGATTCTAAAGT+TGG - chr5_3:18428103-18428122 Msa0803350:intron 25.0%
!!! ATGATTCTGATTCTAAAGTT+GGG - chr5_3:18428104-18428123 Msa0803350:intron 25.0%
! ATTTAGATCAAATATGTGTC+TGG + chr5_3:18428373-18428392 None:intergenic 25.0%
!! TTAATATTAAAGGTGTGTCT+GGG + chr5_3:18428539-18428558 None:intergenic 25.0%
!! ATTAATATTAAAGGTGTGTC+TGG + chr5_3:18428540-18428559 None:intergenic 25.0%
! ATTAATTGTTTGTGCTACAT+AGG - chr5_3:18428554-18428573 Msa0803350:CDS 25.0%
!!! AAGATTTTGACCTTTAGATT+GGG - chr5_3:18428865-18428884 Msa0803350:intron 25.0%
! TATCCTTAATACCATATTCA+TGG - chr5_3:18429005-18429024 Msa0803350:intron 25.0%
! TTTCCATGAATATGGTATTA+AGG + chr5_3:18429011-18429030 None:intergenic 25.0%
!! TTGCATATCTTTTCAATGAA+AGG - chr5_3:18429036-18429055 Msa0803350:CDS 25.0%
! TAGAATTGTCTGTCATTAAT+TGG - chr5_3:18429062-18429081 Msa0803350:CDS 25.0%
! AGTAGTGAGTGTTATATTAT+TGG - chr5_3:18429091-18429110 Msa0803350:CDS 25.0%
!! TTAAAGGTTCTATATTTTGC+AGG + chr5_3:18429117-18429136 None:intergenic 25.0%
!!! AGGTTAGTTTCTTTTACTTT+TGG - chr5_3:18429145-18429164 Msa0803350:CDS 25.0%
!!! TTAGTTTCTTTTACTTTTGG+TGG - chr5_3:18429148-18429167 Msa0803350:CDS 25.0%
!!! GACATGTTTTTCAAATTTTG+AGG - chr5_3:18429270-18429289 Msa0803350:CDS 25.0%
!!! ATGTTTTTCAAATTTTGAGG+AGG - chr5_3:18429273-18429292 Msa0803350:CDS 25.0%
! GAAGCTGAAAAATAAATACA+AGG + chr5_3:18429424-18429443 None:intergenic 25.0%
!!! CATTTTCTATTTCTAGACTT+AGG + chr5_3:18429496-18429515 None:intergenic 25.0%
! TCAGTATAAAGAATTCACAA+GGG + chr5_3:18429806-18429825 None:intergenic 25.0%
! AGTTTGCAAAAACAGTAAAA+TGG + chr5_3:18430131-18430150 None:intergenic 25.0%
!! TGTTTTCTTTGCATATAGTT+AGG - chr5_3:18430167-18430186 Msa0803350:intron 25.0%
!! GTTTTCTTTGCATATAGTTA+GGG - chr5_3:18430168-18430187 Msa0803350:intron 25.0%
! GGCTTATTTAATAATTGCAT+TGG - chr5_3:18430189-18430208 Msa0803350:intron 25.0%
! GTAATATCTGGTAATTATTC+AGG - chr5_3:18430242-18430261 Msa0803350:intron 25.0%
! TGAAAATATTGAAAAGAGTG+GGG - chr5_3:18430458-18430477 Msa0803350:intron 25.0%
!! TCAATAACATCATCATTTTC+AGG + chr5_3:18430546-18430565 None:intergenic 25.0%
!!! TGAAGATTTTTCTGATTTGA+TGG + chr5_3:18430603-18430622 None:intergenic 25.0%
! CAGAAAAATCTTCATTTGAA+TGG - chr5_3:18430609-18430628 Msa0803350:intron 25.0%
!! AAAAAGTATATTCAATCACC+TGG + chr5_3:18430828-18430847 None:intergenic 25.0%
!!! GGTGATTGAATATACTTTTT+TGG - chr5_3:18430828-18430847 Msa0803350:intron 25.0%
!!! TCTTTTTTCTCTTGTTTTCT+GGG + chr5_3:18431092-18431111 None:intergenic 25.0%
!! ATTGAATAGTCTTTGACTTT+AGG - chr5_3:18431208-18431227 Msa0803350:CDS 25.0%
GAAGAAATGAAATTCTTGGT+TGG + chr5_3:18427596-18427615 None:intergenic 30.0%
ATTGAGAGAAAGAGAAGTTT+AGG + chr5_3:18427624-18427643 None:intergenic 30.0%
! ATGGTGATTTGTCAGATATT+TGG - chr5_3:18427927-18427946 Msa0803350:CDS 30.0%
AAAATCCGAGTAGAATTAAC+TGG + chr5_3:18427989-18428008 None:intergenic 30.0%
! AGTTAATTCTACTCGGATTT+TGG - chr5_3:18427988-18428007 Msa0803350:CDS 30.0%
! GAACTGGTTGTATGATTTTA+AGG - chr5_3:18428060-18428079 Msa0803350:CDS 30.0%
AATTCAGATCAATTGTGTGT+CGG + chr5_3:18428285-18428304 None:intergenic 30.0%
ATCCAGATTAAATGTGTGTT+TGG + chr5_3:18428448-18428467 None:intergenic 30.0%
AATCACTGATACAAAATCAC+AGG + chr5_3:18428473-18428492 None:intergenic 30.0%
! TAATATTAAAGGTGTGTCTG+GGG + chr5_3:18428538-18428557 None:intergenic 30.0%
!! CTACATAGGTTTTGTCTTTT+TGG - chr5_3:18428568-18428587 Msa0803350:CDS 30.0%
!! GAAGATTTTGACCTTTAGAT+TGG - chr5_3:18428864-18428883 Msa0803350:intron 30.0%
CAAGAAAGTTTCCATGAATA+TGG + chr5_3:18429019-18429038 None:intergenic 30.0%
GAAAATTCTGATACAAGTCA+TGG + chr5_3:18429181-18429200 None:intergenic 30.0%
!! TTTTAGAAGACATATGCAAG+AGG - chr5_3:18429207-18429226 Msa0803350:intron 30.0%
AATATTGCGAAGAAAGTCTT+TGG - chr5_3:18429298-18429317 Msa0803350:CDS 30.0%
CATACCATTCTGAATCAAAA+AGG + chr5_3:18429381-18429400 None:intergenic 30.0%
! AGATACTATTTCAAGTGCAT+TGG - chr5_3:18429517-18429536 Msa0803350:CDS 30.0%
TGATGCTAAGATTAAATGTG+AGG - chr5_3:18429565-18429584 Msa0803350:intron 30.0%
GATGCTAAGATTAAATGTGA+GGG - chr5_3:18429566-18429585 Msa0803350:intron 30.0%
CTGTCTTAAACCTCTTTAAA+TGG + chr5_3:18429658-18429677 None:intergenic 30.0%
GTCAGTATAAAGAATTCACA+AGG + chr5_3:18429807-18429826 None:intergenic 30.0%
TGCCTGTCAACATAAAAAAT+GGG + chr5_3:18429829-18429848 None:intergenic 30.0%
ATGCCTGTCAACATAAAAAA+TGG + chr5_3:18429830-18429849 None:intergenic 30.0%
AAAAGTTGGCTGTAACTTTA+TGG - chr5_3:18430037-18430056 Msa0803350:CDS 30.0%
!!! TTTCCTCAAGATTTTTTCCT+AGG - chr5_3:18430078-18430097 Msa0803350:CDS 30.0%
GGTAATTATTCAGGTAACTA+AGG - chr5_3:18430251-18430270 Msa0803350:intron 30.0%
AGACTTCTCAGAAAATTCTT+TGG + chr5_3:18430493-18430512 None:intergenic 30.0%
!!! AGACTGCATTTGTTTTTATC+AGG + chr5_3:18430732-18430751 None:intergenic 30.0%
TTGTTCAATAACCATTGAGA+TGG - chr5_3:18430882-18430901 Msa0803350:CDS 30.0%
!!! CTTTTTTCTCTTGTTTTCTG+GGG + chr5_3:18431091-18431110 None:intergenic 30.0%
!!! GTCTTTTTTCTCTTGTTTTC+TGG + chr5_3:18431093-18431112 None:intergenic 30.0%
AACAAAAAGACCACAGATAA+AGG + chr5_3:18431271-18431290 None:intergenic 30.0%
TGTCATGACTACATACAATT+TGG + chr5_3:18431311-18431330 None:intergenic 30.0%
ACAGATACAAATAGAAATCG+CGG - chr5_3:18431360-18431379 Msa0803350:five_prime_UTR 30.0%
AAAGAAATACCCAATTGAGC+AGG + chr5_3:18427779-18427798 None:intergenic 35.0%
TGCTCAATTGGGTATTTCTT+TGG - chr5_3:18427778-18427797 Msa0803350:three_prime_UTR 35.0%
GACGTTTATGTTGATGTTGT+CGG - chr5_3:18427821-18427840 Msa0803350:CDS 35.0%
CTTAACCAACGATGAGTTTA+TGG - chr5_3:18427868-18427887 Msa0803350:CDS 35.0%
! TGATTTGTCAGATATTTGGC+CGG - chr5_3:18427931-18427950 Msa0803350:CDS 35.0%
AAAACGCTTACAATCGTCAA+TGG + chr5_3:18428130-18428149 None:intergenic 35.0%
ATTCAGATCAATTGTGTGTC+GGG + chr5_3:18428284-18428303 None:intergenic 35.0%
CTGAATTGTATGTGCTACAT+AGG - chr5_3:18428298-18428317 Msa0803350:CDS 35.0%
CACCAAACACACATTTAATC+TGG - chr5_3:18428443-18428462 Msa0803350:CDS 35.0%
! TTGACGATCTTTTGTTCAAC+AGG - chr5_3:18428604-18428623 Msa0803350:CDS 35.0%
! TGACGATCTTTTGTTCAACA+GGG - chr5_3:18428605-18428624 Msa0803350:CDS 35.0%
ACCTTTAGATTGGGAATTTC+AGG - chr5_3:18428874-18428893 Msa0803350:intron 35.0%
ACCTGAAATTCCCAATCTAA+AGG + chr5_3:18428878-18428897 None:intergenic 35.0%
! TTGGGAATTTCAGGTCATAA+TGG - chr5_3:18428883-18428902 Msa0803350:intron 35.0%
! TGGGAATTTCAGGTCATAAT+GGG - chr5_3:18428884-18428903 Msa0803350:intron 35.0%
AATTATCCACGAATACCACA+GGG + chr5_3:18428978-18428997 None:intergenic 35.0%
AAATTATCCACGAATACCAC+AGG + chr5_3:18428979-18428998 None:intergenic 35.0%
!! GTCTCCTTTTTGATTCAGAA+TGG - chr5_3:18429374-18429393 Msa0803350:CDS 35.0%
TCTAGACTTAGGTCAATCAA+TGG + chr5_3:18429485-18429504 None:intergenic 35.0%
AAATGGAATGCAGCAATTGA+AGG + chr5_3:18429641-18429660 None:intergenic 35.0%
TTGCTGCATTCCATTTAAAG+AGG - chr5_3:18429645-18429664 Msa0803350:intron 35.0%
!!! GACCCATTTTTTATGTTGAC+AGG - chr5_3:18429824-18429843 Msa0803350:intron 35.0%
TATGCAGTTGTTGTGCATAA+TGG - chr5_3:18429867-18429886 Msa0803350:intron 35.0%
! GCATAAGTTGGATGATTCAA+TGG - chr5_3:18429947-18429966 Msa0803350:intron 35.0%
TCTGCTATACTTGTTCTACT+TGG - chr5_3:18429975-18429994 Msa0803350:intron 35.0%
AGTTTCAAGCTATGAAGACT+GGG + chr5_3:18430106-18430125 None:intergenic 35.0%
TAGTTTCAAGCTATGAAGAC+TGG + chr5_3:18430107-18430126 None:intergenic 35.0%
! AGGTAACTAAGGTATCTGAA+GGG - chr5_3:18430262-18430281 Msa0803350:intron 35.0%
!! ATATTGTTTTATGCCCGACA+AGG - chr5_3:18430309-18430328 Msa0803350:CDS 35.0%
!!! ACCTTGGTTTTCAAGTTTTG+CGG - chr5_3:18430335-18430354 Msa0803350:CDS 35.0%
! GTGATGTCTAAAACAGACTT+GGG + chr5_3:18430405-18430424 None:intergenic 35.0%
! AGTGATGTCTAAAACAGACT+TGG + chr5_3:18430406-18430425 None:intergenic 35.0%
!! TCTGTTTTAGACATCACTGA+TGG - chr5_3:18430408-18430427 Msa0803350:intron 35.0%
ATAATTCACAGGTAGCACTT+GGG + chr5_3:18430652-18430671 None:intergenic 35.0%
AGTGGTAAAAGATCATCATC+AGG - chr5_3:18430967-18430986 Msa0803350:CDS 35.0%
!! TTCAAGGAGAAGTGCATTTT+TGG - chr5_3:18431050-18431069 Msa0803350:CDS 35.0%
!! AGTGCATTTTTGGACTTACT+TGG - chr5_3:18431060-18431079 Msa0803350:CDS 35.0%
! AAAGACTGATTCATCCAAGT+CGG - chr5_3:18431107-18431126 Msa0803350:CDS 35.0%
! AGTCTTTGACTTTAGGCATT+TGG - chr5_3:18431215-18431234 Msa0803350:CDS 35.0%
GGTGAAAATACCTTTATCTG+TGG - chr5_3:18431258-18431277 Msa0803350:CDS 35.0%
! TACAATTTGGCTAGAGGTAT+TGG + chr5_3:18431298-18431317 None:intergenic 35.0%
ACTACATACAATTTGGCTAG+AGG + chr5_3:18431304-18431323 None:intergenic 35.0%
! TTCTCATGCATAATGGAACA+GGG + chr5_3:18431341-18431360 None:intergenic 35.0%
GTATCTGTTCTCATGCATAA+TGG + chr5_3:18431348-18431367 None:intergenic 35.0%
TCTGATTCAAATTCACCACG+TGG - chr5_3:18427683-18427702 Msa0803350:three_prime_UTR 40.0%
TTTATGGCTGTGATGTACAC+CGG - chr5_3:18427884-18427903 Msa0803350:CDS 40.0%
GATTTGTCAGATATTTGGCC+GGG - chr5_3:18427932-18427951 Msa0803350:CDS 40.0%
ATTTGTCAGATATTTGGCCG+GGG - chr5_3:18427933-18427952 Msa0803350:CDS 40.0%
CGAGTAGAATTAACTGGAAC+CGG + chr5_3:18427983-18428002 None:intergenic 40.0%
CGGTTCCAGTTAATTCTACT+CGG - chr5_3:18427981-18428000 Msa0803350:CDS 40.0%
! TCATCATCAGGAGCATAATC+CGG + chr5_3:18428040-18428059 None:intergenic 40.0%
TACAACCAGTTCTCATCATC+AGG + chr5_3:18428052-18428071 None:intergenic 40.0%
CTAAATTGTCTGTGCTACAG+AGG - chr5_3:18428387-18428406 Msa0803350:CDS 40.0%
AAAGCATGTGTTACCAAGGT+TGG + chr5_3:18428642-18428661 None:intergenic 40.0%
! TGAGAAAGCATGTGTTACCA+AGG + chr5_3:18428646-18428665 None:intergenic 40.0%
AAAAGAAGACCCTCAACTAG+GGG + chr5_3:18428701-18428720 None:intergenic 40.0%
CAAAAGAAGACCCTCAACTA+GGG + chr5_3:18428702-18428721 None:intergenic 40.0%
TCAAAAGAAGACCCTCAACT+AGG + chr5_3:18428703-18428722 None:intergenic 40.0%
ACTCACCATCATTAGGAGAA+TGG + chr5_3:18428730-18428749 None:intergenic 40.0%
! TCCTAATGATGGTGAGTAAG+AGG - chr5_3:18428733-18428752 Msa0803350:intron 40.0%
TCCTCTTACTCACCATCATT+AGG + chr5_3:18428737-18428756 None:intergenic 40.0%
AGGACTGTTTCATCTGATAG+CGG + chr5_3:18428930-18428949 None:intergenic 40.0%
AAACAGTCCTTGCAGTTTAC+TGG - chr5_3:18428940-18428959 Msa0803350:intron 40.0%
AACTCTTCCAGTAAACTGCA+AGG + chr5_3:18428950-18428969 None:intergenic 40.0%
ATTATCCACGAATACCACAG+GGG + chr5_3:18428977-18428996 None:intergenic 40.0%
TGAGTTTATGCAGTGTGCAT+TGG - chr5_3:18429237-18429256 Msa0803350:intron 40.0%
ATTGCGAAGAAAGTCTTTGG+AGG - chr5_3:18429301-18429320 Msa0803350:CDS 40.0%
TGCATTGGAGTCTTTCACAA+TGG - chr5_3:18429532-18429551 Msa0803350:CDS 40.0%
ATTGGAGTCTTTCACAATGG+TGG - chr5_3:18429535-18429554 Msa0803350:CDS 40.0%
!! CTGCAATGAAGAAGTGTCTA+TGG - chr5_3:18429589-18429608 Msa0803350:intron 40.0%
TATGGAGAAGCAGCTTATGT+TGG - chr5_3:18429607-18429626 Msa0803350:intron 40.0%
CAAGCATATCAATTTCCCAC+TGG - chr5_3:18429703-18429722 Msa0803350:CDS 40.0%
TATCAATTTCCCACTGGAGT+TGG - chr5_3:18429709-18429728 Msa0803350:CDS 40.0%
AATGGAACTTCATCTGACTC+TGG - chr5_3:18429885-18429904 Msa0803350:intron 40.0%
ATGGAACTTCATCTGACTCT+GGG - chr5_3:18429886-18429905 Msa0803350:intron 40.0%
GCTCCTAGGAAAAAATCTTG+AGG + chr5_3:18430084-18430103 None:intergenic 40.0%
! CAGGTAACTAAGGTATCTGA+AGG - chr5_3:18430261-18430280 Msa0803350:intron 40.0%
TCCGCAAAACTTGAAAACCA+AGG + chr5_3:18430339-18430358 None:intergenic 40.0%
TTCCATGCTTGAATCTGAGT+AGG - chr5_3:18430367-18430386 Msa0803350:CDS 40.0%
ATCCTACTCAGATTCAAGCA+TGG + chr5_3:18430372-18430391 None:intergenic 40.0%
AATATTGAAAAGAGTGGGGC+TGG - chr5_3:18430462-18430481 Msa0803350:intron 40.0%
CATAATTCACAGGTAGCACT+TGG + chr5_3:18430653-18430672 None:intergenic 40.0%
! ACCATTGAGATGGATTTGTC+AGG - chr5_3:18430892-18430911 Msa0803350:CDS 40.0%
ACCTGACAAATCCATCTCAA+TGG + chr5_3:18430896-18430915 None:intergenic 40.0%
CATTTGAAGACAGAGAAGCA+AGG - chr5_3:18430940-18430959 Msa0803350:CDS 40.0%
ATCATCATCAGGCAAGTCTT+CGG - chr5_3:18430978-18430997 Msa0803350:CDS 40.0%
ATCCTCAAAATAAAGCTGCC+AGG - chr5_3:18431004-18431023 Msa0803350:CDS 40.0%
TCCTCAAAATAAAGCTGCCA+GGG - chr5_3:18431005-18431024 Msa0803350:CDS 40.0%
CATATTCCTAACATATGCCC+TGG + chr5_3:18431025-18431044 None:intergenic 40.0%
GCATATGTTAGGAATATGCC+TGG - chr5_3:18431027-18431046 Msa0803350:CDS 40.0%
TTAGGAATATGCCTGGTTCA+AGG - chr5_3:18431034-18431053 Msa0803350:CDS 40.0%
!! GCATTTTTGGACTTACTTGG+TGG - chr5_3:18431063-18431082 Msa0803350:CDS 40.0%
!!! TGGCTGTGTACTTGGTTTTT+TGG - chr5_3:18431235-18431254 Msa0803350:CDS 40.0%
!!! GGCTGTGTACTTGGTTTTTT+GGG - chr5_3:18431236-18431255 Msa0803350:CDS 40.0%
!!! GCTGTGTACTTGGTTTTTTG+GGG - chr5_3:18431237-18431256 Msa0803350:CDS 40.0%
! GTTCTCATGCATAATGGAAC+AGG + chr5_3:18431342-18431361 None:intergenic 40.0%
ACAAATAGAAATCGCGGACA+AGG - chr5_3:18431366-18431385 Msa0803350:five_prime_UTR 40.0%
TGTTGTTCTTGTTCACCACG+TGG + chr5_3:18427701-18427720 None:intergenic 45.0%
AACCGTAGATCCTGCTCAAT+TGG - chr5_3:18427766-18427785 Msa0803350:three_prime_UTR 45.0%
ACCGTAGATCCTGCTCAATT+GGG - chr5_3:18427767-18427786 Msa0803350:three_prime_UTR 45.0%
ACCCAATTGAGCAGGATCTA+CGG + chr5_3:18427771-18427790 None:intergenic 45.0%
AGTTTCTGAGCCTGAGGATA+TGG - chr5_3:18427844-18427863 Msa0803350:CDS 45.0%
GTTTCTGAGCCTGAGGATAT+GGG - chr5_3:18427845-18427864 Msa0803350:CDS 45.0%
TTGGTTAAGCCCATATCCTC+AGG + chr5_3:18427857-18427876 None:intergenic 45.0%
CACAGCCATAAACTCATCGT+TGG + chr5_3:18427876-18427895 None:intergenic 45.0%
AAATCACCATGATCTCGCTG+CGG + chr5_3:18427917-18427936 None:intergenic 45.0%
TTTGTCAGATATTTGGCCGG+GGG - chr5_3:18427934-18427953 Msa0803350:CDS 45.0%
! ATGCTCCTGATGATGAGAAC+TGG - chr5_3:18428044-18428063 Msa0803350:CDS 45.0%
ATTGTCCACCGACACATGTT+AGG - chr5_3:18428420-18428439 Msa0803350:CDS 45.0%
!! GTTTGGTGCCTAACATGTGT+CGG + chr5_3:18428431-18428450 None:intergenic 45.0%
! TCTTTTGTTCAACAGGGAGC+TGG - chr5_3:18428611-18428630 Msa0803350:CDS 45.0%
! CTTTTGTTCAACAGGGAGCT+GGG - chr5_3:18428612-18428631 Msa0803350:CDS 45.0%
AGCTGCCATTCTCCTAATGA+TGG - chr5_3:18428722-18428741 Msa0803350:CDS 45.0%
! GAAAGTCTTTGGAGGCAGTT+TGG - chr5_3:18429309-18429328 Msa0803350:CDS 45.0%
GCTCATTAGTGTCAGAAGAG+TGG + chr5_3:18429463-18429482 None:intergenic 45.0%
GTTGCAAATCCAACTCCAGT+GGG + chr5_3:18429721-18429740 None:intergenic 45.0%
TCCAGACACATGGCATAAGT+TGG - chr5_3:18429935-18429954 Msa0803350:intron 45.0%
TCCAACTTATGCCATGTGTC+TGG + chr5_3:18429939-18429958 None:intergenic 45.0%
AAAACCAAGGTGTGCCTTGT+CGG + chr5_3:18430326-18430345 None:intergenic 45.0%
TTTAGACATCACTGATGGCC+AGG - chr5_3:18430413-18430432 Msa0803350:intron 45.0%
AATCACCTGGAGTCTGAGAA+GGG + chr5_3:18430815-18430834 None:intergenic 45.0%
TCAAATGATCGCGTGAGATC+TGG + chr5_3:18430927-18430946 None:intergenic 45.0%
ACAGAGAAGCAAGGAAGTAG+TGG - chr5_3:18430949-18430968 Msa0803350:CDS 45.0%
AATGCACTTCTCCTTGAACC+AGG + chr5_3:18431048-18431067 None:intergenic 45.0%
TAGGCATTTGGCTGTGTACT+TGG - chr5_3:18431227-18431246 Msa0803350:CDS 45.0%
! GGATCTTGCTGTGTATGCTC+CGG - chr5_3:18427961-18427980 Msa0803350:CDS 50.0%
! TCATCAGGAGCATAATCCGG+TGG + chr5_3:18428037-18428056 None:intergenic 50.0%
!! CGGGTATTGACACGTGTAAG+TGG + chr5_3:18428265-18428284 None:intergenic 50.0%
CATACTGTGCCCCTAGTTGA+GGG - chr5_3:18428689-18428708 Msa0803350:CDS 50.0%
TGGAAGAGTTATCTCCCCTG+TGG - chr5_3:18428960-18428979 Msa0803350:intron 50.0%
TATCTCCCCTGTGGTATTCG+TGG - chr5_3:18428969-18428988 Msa0803350:intron 50.0%
TGGAGGCAGTTTGGTTAGCA+AGG - chr5_3:18429318-18429337 Msa0803350:CDS 50.0%
GGTTGCAAATCCAACTCCAG+TGG + chr5_3:18429722-18429741 None:intergenic 50.0%
AAACCAAGGTGTGCCTTGTC+GGG + chr5_3:18430325-18430344 None:intergenic 50.0%
! TTCTGATTTGATGGCCCAGC+AGG + chr5_3:18430594-18430613 None:intergenic 50.0%
TCAGCATATGAACTCCCACC+AGG + chr5_3:18430699-18430718 None:intergenic 50.0%
ATCACCTGGAGTCTGAGAAG+GGG + chr5_3:18430814-18430833 None:intergenic 50.0%
CAATCACCTGGAGTCTGAGA+AGG + chr5_3:18430816-18430835 None:intergenic 50.0%
!!! GCCCTGGCAGCTTTATTTTG+AGG + chr5_3:18431009-18431028 None:intergenic 50.0%
AAGCTGCCAGGGCATATGTT+AGG - chr5_3:18431016-18431035 Msa0803350:CDS 50.0%
AGCAGTGCTCGCAAGAAATC+AGG - chr5_3:18431141-18431160 Msa0803350:CDS 50.0%
AAATCAGGCCATGCTTCCGT+CGG - chr5_3:18431156-18431175 Msa0803350:CDS 50.0%
TGTCGGAGTTTCTGAGCCTG+AGG - chr5_3:18427838-18427857 Msa0803350:CDS 55.0%
ATCTCGCTGCGGAACATCAC+CGG + chr5_3:18427906-18427925 None:intergenic 55.0%
GATGTTCCGCAGCGAGATCA+TGG - chr5_3:18427908-18427927 Msa0803350:CDS 55.0%
TCCGGTGGATACCAAGCTTC+CGG + chr5_3:18428022-18428041 None:intergenic 55.0%
TGGTGCCTAACATGTGTCGG+TGG + chr5_3:18428428-18428447 None:intergenic 55.0%
GCATACTGTGCCCCTAGTTG+AGG - chr5_3:18428688-18428707 Msa0803350:CDS 55.0%
TTCGCACTGCTCCAGACACA+TGG - chr5_3:18429925-18429944 Msa0803350:intron 55.0%
GCTATGAAGACTGGGCTCCT+AGG + chr5_3:18430098-18430117 None:intergenic 55.0%
TGAAGGGACTGTGCTGTCTC+AGG - chr5_3:18430278-18430297 Msa0803350:CDS 55.0%
! GCAGGAACTTGTTTGCGGCA+AGG + chr5_3:18430576-18430595 None:intergenic 55.0%
TGCCGCAAACAAGTTCCTGC+TGG - chr5_3:18430576-18430595 Msa0803350:intron 55.0%
GCCGCAAACAAGTTCCTGCT+GGG - chr5_3:18430577-18430596 Msa0803350:intron 55.0%
! GCCCAGCAGGAACTTGTTTG+CGG + chr5_3:18430581-18430600 None:intergenic 55.0%
!! GTCGGGCTGGCATAATTCAC+AGG + chr5_3:18430663-18430682 None:intergenic 55.0%
GTGAATTATGCCAGCCCGAC+TGG - chr5_3:18430663-18430682 Msa0803350:intron 55.0%
CAGGCTTTGCAATGCCAGTC+GGG + chr5_3:18430680-18430699 None:intergenic 55.0%
CCAGGCTTTGCAATGCCAGT+CGG + chr5_3:18430681-18430700 None:intergenic 55.0%
ACTGGCATTGCAAAGCCTGG+TGG - chr5_3:18430681-18430700 Msa0803350:intron 55.0%
CTGGCATTGCAAAGCCTGGT+GGG - chr5_3:18430682-18430701 Msa0803350:intron 55.0%
TCACCTGGAGTCTGAGAAGG+GGG + chr5_3:18430813-18430832 None:intergenic 55.0%
GCTGCTGTCTTTGTCCGACT+TGG + chr5_3:18431124-18431143 None:intergenic 55.0%
GTGAAACTCCAGCTGCCACA+GGG + chr5_3:18431188-18431207 None:intergenic 55.0%
TGTGAAACTCCAGCTGCCAC+AGG + chr5_3:18431189-18431208 None:intergenic 55.0%
AGATATTTGGCCGGGGGCGA+TGG - chr5_3:18427940-18427959 Msa0803350:CDS 60.0%
ACAGCAAGATCCATCGCCCC+CGG + chr5_3:18427953-18427972 None:intergenic 60.0%
!!! TCGGATTTTGGCTGTGCCGC+CGG - chr5_3:18428000-18428019 Msa0803350:CDS 60.0%
GGTGGATACCAAGCTTCCGG+CGG + chr5_3:18428019-18428038 None:intergenic 60.0%
GCCGGAAGCTTGGTATCCAC+CGG - chr5_3:18428018-18428037 Msa0803350:CDS 60.0%
ATGCCCGACAAGGCACACCT+TGG - chr5_3:18430319-18430338 Msa0803350:CDS 60.0%
CTTTGCAATGCCAGTCGGGC+TGG + chr5_3:18430676-18430695 None:intergenic 60.0%
CCGACTGGCATTGCAAAGCC+TGG - chr5_3:18430678-18430697 Msa0803350:intron 60.0%
GGGGGAGTTGGGAGGAGTTA+AGG + chr5_3:18430793-18430812 None:intergenic 60.0%
GTCTGAGAAGGGGGGGAGTT+GGG + chr5_3:18430804-18430823 None:intergenic 60.0%
AGTCTGAGAAGGGGGGGAGT+TGG + chr5_3:18430805-18430824 None:intergenic 60.0%
ACCTGGAGTCTGAGAAGGGG+GGG + chr5_3:18430811-18430830 None:intergenic 60.0%
CACCTGGAGTCTGAGAAGGG+GGG + chr5_3:18430812-18430831 None:intergenic 60.0%
GGAGCTGGGCTTGCCAACCT+TGG - chr5_3:18428626-18428645 Msa0803350:CDS 65.0%
TGAGAAGGGGGGGAGTTGGG+AGG + chr5_3:18430801-18430820 None:intergenic 65.0%
TCCCCCCCTTCTCAGACTCC+AGG - chr5_3:18430807-18430826 Msa0803350:intron 65.0%
GGTAGCTGCCGACGGAAGCA+TGG + chr5_3:18431167-18431186 None:intergenic 65.0%
TTCCGTCGGCAGCTACCCTG+TGG - chr5_3:18431170-18431189 Msa0803350:CDS 65.0%
TGCCACAGGGTAGCTGCCGA+CGG + chr5_3:18431175-18431194 None:intergenic 65.0%
TGGCTGTGCCGCCGGAAGCT+TGG - chr5_3:18428008-18428027 Msa0803350:CDS 70.0%
GGCAGCTACCCTGTGGCAGC+TGG - chr5_3:18431177-18431196 Msa0803350:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 18427586 18431427 18427586 ID=Msa0803350;Name=Msa0803350
chr5_3 mRNA 18427586 18431427 18427586 ID=Msa0803350-mRNA-1;Parent=Msa0803350;Name=Msa0803350-mRNA-1;_AED=0.19;_eAED=0.19;_QI=94|0.85|0.87|1|1|1|8|215|721
chr5_3 exon 18427586 18428099 18427586 ID=Msa0803350-mRNA-1:exon:7551;Parent=Msa0803350-mRNA-1
chr5_3 exon 18428185 18428749 18428185 ID=Msa0803350-mRNA-1:exon:7550;Parent=Msa0803350-mRNA-1
chr5_3 exon 18429027 18429167 18429027 ID=Msa0803350-mRNA-1:exon:7549;Parent=Msa0803350-mRNA-1
chr5_3 exon 18429244 18429573 18429244 ID=Msa0803350-mRNA-1:exon:7548;Parent=Msa0803350-mRNA-1
chr5_3 exon 18429674 18429819 18429674 ID=Msa0803350-mRNA-1:exon:7547;Parent=Msa0803350-mRNA-1
chr5_3 exon 18430010 18430117 18430010 ID=Msa0803350-mRNA-1:exon:7546;Parent=Msa0803350-mRNA-1
chr5_3 exon 18430270 18430387 18430270 ID=Msa0803350-mRNA-1:exon:7545;Parent=Msa0803350-mRNA-1
chr5_3 exon 18430875 18431427 18430875 ID=Msa0803350-mRNA-1:exon:7544;Parent=Msa0803350-mRNA-1
chr5_3 five_prime_UTR 18431334 18431427 18431334 ID=Msa0803350-mRNA-1:five_prime_utr;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18430875 18431333 18430875 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18430270 18430387 18430270 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18430010 18430117 18430010 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18429674 18429819 18429674 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18429244 18429573 18429244 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18429027 18429167 18429027 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18428185 18428749 18428185 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 CDS 18427801 18428099 18427801 ID=Msa0803350-mRNA-1:cds;Parent=Msa0803350-mRNA-1
chr5_3 three_prime_UTR 18427586 18427800 18427586 ID=Msa0803350-mRNA-1:three_prime_utr;Parent=Msa0803350-mRNA-1
Gene Sequence

>Msa0803350

ATGTCTGATTCAAATTCACCACGTGGTGAACAAGAACAACAAGAGAATCAATTGAATTCATCTTCAACGATGAGTTTAATTTCTGAAACCGTAGATCCTGCTCAATTGGGTATTTCTTTGGCTGATCTTGATGATGACATTGACGTTTATGTTGATGTTGTCGGAGTTTCTGAGCCTGAGGATATGGGCTTAACCAACGATGAGTTTATGGCTGTGATGTACACCGGTGATGTTCCGCAGCGAGATCATGGTGATTTGTCAGATATTTGGCCGGGGGCGATGGATCTTGCTGTGTATGCTCCGGTTCCAGTTAATTCTACTCGGATTTTGGCTGTGCCGCCGGAAGCTTGGTATCCACCGGATTATGCTCCTGATGATGAGAACTGGTTGTATGATTTTAAGGAATCCTTAAAGGATGAAGTTTATGATTCTGATTCTAAAGTTGGGCCATTGACGATTGGAGCTGGGCTTGCCAACCTTGGTAACACATGCTTTCTCAATTCAATTCTGCAATGCTTCACGCATACTGTGCCCCTAGTTGAGGGTCTTCTTTTGAGCTGCCATTCTCCTAATGATGGTCATAATGGGTTCTGTGTTATCTGTGCTTTCCGCTATCAGATGAAACAGTCCTTGCAGTTTACTGGAAGAGTTATCTCCCCTGTGGTATTCGTGGATAATTTGAAGCAATTTTCTAATGATTTTAGAAGACATATGCAAGAGGATGCACATGAGTTTATGCAGTGTGCATTGGATAAACTTGAGACATGTTTTTCAAATTTTGAGGAGGATAATATTGCGAAGAAAGTCTTTGGAGGCAGTTTGGTTAGCAAGCTTCAGTGTTGTAACTGTAGCCACTCTTCTGACACTAATGAGCCATTGATTGACCTAAGTCTAGAAATAGAAAATGTAGATACTATTTCAAGTGCATTGGAGTCTTTCACAATGGTGGAAAAGATTGATGCTAAGATTAAATGTGAGGGCTGCAATGAAGAAGTGTCTATGGAGAAGCAGCTTATGTTGGATCAGACACCTTCAATTGCTGCATTCCATTTAAAGAGGTTTAAGACAGTTGAGAAGTCAGTTGAAAAGATTGACAAGCATATCAATTTCCCACTGGAGTTGGATTTGCAACCTTACACTATTTTGAATGAAAACAATGATGCATCATTGAAATATGATCTATATGCAGTTGTTGTGCATAATGGAACTTCATCTGACTCTGGGCATTATTTCTGCTTTGTTCGCACTGCTCCAGACACATGGCATAAGTTGGATGATTCAATGGTAGCATCTGCTATACTTGTAACTAAGGTATCTGAAGGGACTGTGCTGTCTCAGGAAGCATACATATTGTTTTATGCCCGACAAGGCACACCTTGGTTTTCAAGTTTTGCGGAATCTACAATTCCATGCTTGAATCTGAGTAGGATGAACACGTCTCCCAAGTCTGTTTTAGACATCACTGATGGCCAGGATAAATCATTTTCTATTTTAAATGAAAATATTGAAAAGAGTGGGGCTGGTAAATCCAAAGAATTTTCTGAGAAGTCTGATTATTCTTGTCAACAAAGTCGCAAGTTTCCTGAAAATGATGATGTTATTGATGCTTCTCCTTGCCGCAAACAAGTTCCTGCTGGGCCATCAAATCAGAAAAATCTTCATTTGAATGGATCAGAAGATATCAGCGCCCAAGTGCTACCTGTGAATTATGCCAGCCCGACTGGCATTGCAAAGCCTGGTGGGAGTTCATATGCTGAGAATGTTGCTCCTGATAAAAACAAATGCAGTCTGAAAGCTAATGATTTTATAGAGAATGATGTTTTCAATGCCTTAACTCCTCCCAACTCCCCCCCTTCTCAGACTCCAGGTAAGAGTTTCCAGATCTCACGCGATCATTTGAAGACAGAGAAGCAAGGAAGTAGTGGTAAAAGATCATCATCAGGCAAGTCTTCGGATAATCCTCAAAATAAAGCTGCCAGGGCATATGTTAGGAATATGCCTGGTTCAAGGAGAAGTGCATTTTTGGACTTACTTGGTGGTTCCCCAGAAAACAAGAGAAAAAAGACTGATTCATCCAAGTCGGACAAAGACAGCAGCAGTGCTCGCAAGAAATCAGGCCATGCTTCCGTCGGCAGCTACCCTGTGGCAGCTGGAGTTTCACATTGA
Protein sequence

>Msa0803350

MSDSNSPRGEQEQQENQLNSSSTMSLISETVDPAQLGISLADLDDDIDVYVDVVGVSEPEDMGLTNDEFMAVMYTGDVPQRDHGDLSDIWPGAMDLAVYAPVPVNSTRILAVPPEAWYPPDYAPDDENWLYDFKESLKDEVYDSDSKVGPLTIGAGLANLGNTCFLNSILQCFTHTVPLVEGLLLSCHSPNDGHNGFCVICAFRYQMKQSLQFTGRVISPVVFVDNLKQFSNDFRRHMQEDAHEFMQCALDKLETCFSNFEEDNIAKKVFGGSLVSKLQCCNCSHSSDTNEPLIDLSLEIENVDTISSALESFTMVEKIDAKIKCEGCNEEVSMEKQLMLDQTPSIAAFHLKRFKTVEKSVEKIDKHINFPLELDLQPYTILNENNDASLKYDLYAVVVHNGTSSDSGHYFCFVRTAPDTWHKLDDSMVASAILVTKVSEGTVLSQEAYILFYARQGTPWFSSFAESTIPCLNLSRMNTSPKSVLDITDGQDKSFSILNENIEKSGAGKSKEFSEKSDYSCQQSRKFPENDDVIDASPCRKQVPAGPSNQKNLHLNGSEDISAQVLPVNYASPTGIAKPGGSSYAENVAPDKNKCSLKANDFIENDVFNALTPPNSPPSQTPGKSFQISRDHLKTEKQGSSGKRSSSGKSSDNPQNKAARAYVRNMPGSRRSAFLDLLGGSPENKRKKTDSSKSDKDSSSARKKSGHASVGSYPVAAGVSH*