AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0720520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720520 MtrunA17_Chr5g0411271 90.069 725 58 5 1 721 1 715 0.0 1234
Msa0720520 MtrunA17_Chr6g0458431 71.352 562 130 8 153 705 92 631 0.0 743
Msa0720520 MtrunA17_Chr6g0458441 58.242 728 192 14 18 705 1 656 0.0 717
Msa0720520 MtrunA17_Chr6g0458421 67.606 568 129 10 153 712 27 547 0.0 710
Msa0720520 MtrunA17_Chr8g0366721 56.329 316 122 4 152 466 209 509 9.17e-111 347
Msa0720520 MtrunA17_Chr8g0365831 47.516 322 155 5 152 466 161 475 4.21e-91 294
Msa0720520 MtrunA17_Chr4g0074041 42.089 316 160 6 153 455 90 395 5.63e-74 256
Msa0720520 MtrunA17_Chr4g0062561 39.375 320 173 9 141 456 239 541 2.60e-57 210
Msa0720520 MtrunA17_Chr4g0062561 38.356 292 157 8 173 456 577 853 9.96e-48 181
Msa0720520 MtrunA17_Chr4g0062491 35.806 310 178 6 166 463 1 301 4.69e-55 191
Msa0720520 MtrunA17_Chr1g0150541 35.016 317 179 10 155 455 181 486 2.69e-49 186
Msa0720520 MtrunA17_Chr2g0313171 34.412 340 188 11 132 455 449 769 1.57e-47 181
Msa0720520 MtrunA17_Chr3g0133061 34.286 315 181 9 156 455 585 888 1.91e-47 181
Msa0720520 MtrunA17_Chr5g0412381 34.024 338 194 11 133 456 405 727 1.00e-46 179
Msa0720520 MtrunA17_Chr2g0312901 32.698 315 188 7 156 455 23 328 1.82e-46 176
Msa0720520 MtrunA17_Chr5g0427301 33.651 315 182 10 156 455 543 845 1.02e-44 173
Msa0720520 MtrunA17_Chr1g0149991 31.534 352 182 11 156 456 182 525 1.06e-40 157
Msa0720520 MtrunA17_Chr3g0123951 30.347 346 184 10 156 453 24 360 1.43e-35 138
Msa0720520 MtrunA17_Chr8g0382161 30.259 347 191 9 156 457 24 364 1.89e-35 138
Msa0720520 MtrunA17_Chr4g0064671 28.400 250 120 8 258 456 5 246 1.47e-27 112
Msa0720520 MtrunA17_Chr1g0148321 29.352 293 174 9 155 430 107 383 3.58e-24 108
Msa0720520 MtrunA17_Chr3g0138791 28.424 387 243 14 60 431 17 384 1.48e-22 103
Msa0720520 MtrunA17_Chr4g0020741 26.741 359 194 12 156 455 183 531 1.08e-21 99.8
Msa0720520 MtrunA17_Chr5g0439971 26.298 289 192 9 155 431 204 483 1.50e-20 97.4
Msa0720520 MtrunA17_Chr3g0119031 26.736 288 190 10 155 430 199 477 2.42e-20 96.7
Msa0720520 MtrunA17_Chr1g0170261 26.552 290 188 10 155 430 201 479 4.27e-20 95.9
Msa0720520 MtrunA17_Chr2g0322211 33.766 154 90 5 305 455 717 861 3.60e-15 79.7
Msa0720520 MtrunA17_Chr1g0189101 35.443 158 89 6 305 458 765 913 4.93e-15 79.3
Msa0720520 MtrunA17_Chr7g0255591 32.911 158 94 6 305 458 754 903 1.84e-14 77.4
Msa0720520 MtrunA17_Chr3g0121291 35.938 128 73 3 336 455 943 1069 2.16e-14 77.4
Msa0720520 MtrunA17_Chr3g0121291 31.447 159 89 5 156 300 232 384 3.28e-12 70.5
Msa0720520 MtrunA17_Chr7g0267641 33.117 154 92 5 305 455 791 936 4.37e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720520 AT4G17895.1 53.644 343 147 4 150 486 170 506 1.73e-114 361
Msa0720520 AT5G46740.1 38.023 597 303 15 147 696 155 731 2.92e-105 338
Msa0720520 AT5G46740.2 37.222 540 274 14 202 696 3 522 1.13e-84 277
Msa0720520 AT5G57990.1 42.540 315 159 6 153 455 105 409 3.57e-76 263
Msa0720520 AT4G31670.1 35.331 317 178 9 155 455 168 473 3.85e-49 183
Msa0720520 AT5G65450.1 34.921 315 178 9 156 455 330 632 9.46e-48 181
Msa0720520 AT5G65450.2 34.921 315 178 9 156 455 349 651 1.27e-47 180
Msa0720520 AT2G24640.1 32.877 365 211 11 107 455 133 479 2.71e-47 178
Msa0720520 AT3G14400.1 33.856 319 183 8 156 455 25 334 8.67e-47 177
Msa0720520 AT2G24640.2 34.700 317 180 9 155 455 4 309 1.16e-46 173
Msa0720520 AT1G17110.3 32.754 345 194 11 130 455 418 743 3.98e-44 171
Msa0720520 AT1G17110.1 32.754 345 194 11 130 455 418 743 3.98e-44 171
Msa0720520 AT1G17110.2 32.754 345 194 11 130 455 422 747 4.41e-44 171
Msa0720520 AT4G24560.1 31.646 316 188 10 156 455 543 846 2.92e-40 159
Msa0720520 AT2G22310.1 30.659 349 188 11 156 458 24 364 9.51e-34 133
Msa0720520 AT4G39910.1 29.944 354 187 11 156 457 24 368 2.00e-33 132
Msa0720520 AT5G10790.2 27.901 362 192 12 156 456 49 402 1.65e-29 122
Msa0720520 AT5G10790.1 27.901 362 192 12 156 456 178 531 2.16e-28 120
Msa0720520 AT2G22310.3 30.294 340 177 13 168 458 4 332 1.48e-27 114
Msa0720520 AT2G22310.2 31.208 298 151 11 206 458 39 327 1.59e-26 111
Msa0720520 AT4G30890.1 25.208 361 201 10 156 455 198 550 3.74e-23 104
Msa0720520 AT4G30890.2 25.208 361 201 10 156 455 198 550 3.74e-23 104
Msa0720520 AT4G30890.3 25.208 361 201 10 156 455 198 550 3.74e-23 104
Msa0720520 AT3G11910.4 26.351 296 201 9 155 440 197 485 2.57e-22 103
Msa0720520 AT3G11910.2 26.351 296 201 9 155 440 197 485 2.57e-22 103
Msa0720520 AT3G11910.3 26.351 296 201 9 155 440 198 486 2.62e-22 103
Msa0720520 AT3G11910.1 26.351 296 201 9 155 440 198 486 2.62e-22 103
Msa0720520 AT5G06600.3 26.923 286 192 9 155 430 199 477 2.22e-21 100
Msa0720520 AT5G06600.1 26.829 287 193 9 155 431 199 478 2.23e-21 100
Msa0720520 AT5G06600.2 26.829 287 193 9 155 431 198 477 2.25e-21 100
Msa0720520 AT3G49600.2 28.322 286 185 8 155 430 106 381 2.93e-20 96.7
Msa0720520 AT3G49600.1 28.322 286 185 8 155 430 106 381 2.93e-20 96.7
Msa0720520 AT3G49600.3 28.322 286 185 8 155 430 106 381 3.50e-20 96.3
Msa0720520 AT5G22030.1 34.416 154 90 5 305 455 723 868 2.32e-16 84.0
Msa0720520 AT5G22030.2 34.416 154 90 5 305 455 765 910 2.58e-16 84.0
Msa0720520 AT2G40930.1 33.333 156 92 6 305 456 769 916 3.97e-15 80.1
Msa0720520 AT2G40930.2 33.333 156 92 6 305 456 769 916 3.97e-15 80.1
Msa0720520 AT4G10590.4 37.419 155 84 6 305 455 748 893 5.22e-15 79.7
Msa0720520 AT4G10590.4 29.167 192 103 5 155 313 303 494 4.10e-12 70.5
Msa0720520 AT4G10590.3 37.419 155 84 6 305 455 748 893 5.22e-15 79.7
Msa0720520 AT4G10590.3 29.167 192 103 5 155 313 303 494 4.10e-12 70.5
Msa0720520 AT4G10590.1 37.419 155 84 6 305 455 748 893 5.22e-15 79.7
Msa0720520 AT4G10590.1 29.167 192 103 5 155 313 303 494 4.10e-12 70.5
Msa0720520 AT4G10590.2 37.419 155 84 6 305 455 748 893 5.22e-15 79.7
Msa0720520 AT4G10590.2 29.167 192 103 5 155 313 303 494 4.10e-12 70.5
Msa0720520 AT4G10570.1 36.774 155 85 7 305 455 749 894 2.17e-14 77.8
Msa0720520 AT4G10570.1 29.167 192 103 5 155 313 304 495 2.64e-12 70.9
Msa0720520 AT1G32850.1 35.484 155 87 7 305 455 734 879 4.38e-14 76.6
Msa0720520 AT1G32850.1 27.604 192 106 2 155 313 301 492 1.74e-12 71.6
Msa0720520 AT1G04860.1 33.974 156 86 4 156 298 232 383 1.32e-13 75.1

Find 170 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCTTAAACCTCTTTAAA+TGG 0.207305 5_1:+16551583 None:intergenic
AGTTAATTCTACTCGGATTT+TGG 0.210552 5_1:-16553237 Msa0720520:CDS
TGCTCAATTGGGTATTTCTT+TGG 0.217652 5_1:-16553447 Msa0720520:CDS
AGACTTCTCAGAAAATTCTT+TGG 0.249608 5_1:+16550746 None:intergenic
ATTGAGAGAAAGAGAAGTTT+AGG 0.251577 5_1:+16553604 None:intergenic
ATGGTGATTTATCAGATATT+TGG 0.272470 5_1:-16553298 Msa0720520:CDS
AGTTTCTGAGCCTGAGGATA+TGG 0.285559 5_1:-16553381 Msa0720520:CDS
ATTGAATAGTCTTTGACTTT+AGG 0.290779 5_1:-16550028 Msa0720520:exon
TGTCATGACTACATACAATT+TGG 0.304833 5_1:+16549928 None:intergenic
TACAATTTGGCTAGAGGTAT+TGG 0.311108 5_1:+16549941 None:intergenic
AATATTGCGAAGAAAGTCTT+TGG 0.320644 5_1:-16551940 Msa0720520:CDS
AACCGTAGATCCTGCTCAAT+TGG 0.346549 5_1:-16553459 Msa0720520:CDS
AGTCTTTGACTTTAGGCATT+TGG 0.346739 5_1:-16550021 Msa0720520:three_prime_UTR
ATCATAAACTTCATCCTTCA+AGG 0.350177 5_1:+16553139 None:intergenic
TAGGCATTTGGCTGTGTACT+TGG 0.354596 5_1:-16550009 Msa0720520:three_prime_UTR
GTATCTGTTCTCATGCATAA+TGG 0.357638 5_1:+16549891 None:intergenic
GAAGAAATGAAATTCTTTGT+TGG 0.359357 5_1:+16553632 None:intergenic
TTGGGAATTTCAGGTCATAA+TGG 0.374228 5_1:-16552355 Msa0720520:intron
AAAATCCGAGTAGAATTAAC+TGG 0.376969 5_1:+16553239 None:intergenic
GTTCTCATGCATAATGGAAC+AGG 0.386413 5_1:+16549897 None:intergenic
TCAAATGATCGCGTGAGATC+TGG 0.390392 5_1:+16550312 None:intergenic
TGGGAATTTCAGGTCATAAT+GGG 0.391890 5_1:-16552354 Msa0720520:intron
TGCCGCAAACAAGTTCCTGC+TGG 0.392129 5_1:-16550660 Msa0720520:CDS
TATGCAGTTGTTGTGCATAA+TGG 0.393951 5_1:-16551371 Msa0720520:CDS
GGCAGCTACCCTGTGGCAGC+TGG 0.397430 5_1:-16550059 Msa0720520:CDS
AGCAGTGCTCGCAAGAAATC+AGG 0.399732 5_1:-16550095 Msa0720520:CDS
GAAAGTCTTTGGAGGCAGTT+TGG 0.402179 5_1:-16551929 Msa0720520:CDS
CTGCAATGAAGAAGTGTCTA+TGG 0.405794 5_1:-16551649 Msa0720520:CDS
AAACAGTCCTTGCAGTCTAC+TGG 0.408748 5_1:-16552298 Msa0720520:CDS
TTCTGATTTGATGGCCCAGC+AGG 0.410077 5_1:+16550645 None:intergenic
TCCGGTGGATACCAAGCTTC+CGG 0.412997 5_1:+16553206 None:intergenic
AAAACCAAGGTGTGCCTTGT+CGG 0.413813 5_1:+16550913 None:intergenic
GTTTCTGAGCCTGAGGATAT+GGG 0.417439 5_1:-16553380 Msa0720520:CDS
GTGAATTATGCCAGCCCGAC+TGG 0.419613 5_1:-16550573 Msa0720520:CDS
CGAGTAGAATTAACTGGAAC+CGG 0.424615 5_1:+16553245 None:intergenic
TCCAACTTATGCCATGTGTC+TGG 0.425693 5_1:+16551302 None:intergenic
TACAACCAGTTCTCATCATC+AGG 0.433784 5_1:+16553176 None:intergenic
GACGTTTATGTTGATGTTGT+CGG 0.434418 5_1:-16553404 Msa0720520:CDS
TATATCCCCTGTGGTATTTG+TGG 0.437517 5_1:-16552269 Msa0720520:CDS
TGGCTGTGCCGCCGGAAGCT+TGG 0.438381 5_1:-16553217 Msa0720520:CDS
TTTGTCCGACTTGGATGAAT+CGG 0.442350 5_1:+16550124 None:intergenic
TCCTCTTACTCACCATCATT+AGG 0.442960 5_1:+16552504 None:intergenic
AAATAGAAAATGATTTATCC+TGG 0.443696 5_1:+16550805 None:intergenic
CTTTGCAATGCCAGTCGGGC+TGG 0.444918 5_1:+16550563 None:intergenic
CGGTTCCAGTTAATTCTACT+CGG 0.445652 5_1:-16553244 Msa0720520:CDS
GCTGCTGTCTTTGTCCGACT+TGG 0.449405 5_1:+16550115 None:intergenic
AAATGGAATGCAGCAATTGA+AGG 0.458769 5_1:+16551600 None:intergenic
CAGGCTTTGCAATGCCAGTC+GGG 0.460520 5_1:+16550559 None:intergenic
AAGCTGCCAGGGCATATGTT+AGG 0.462405 5_1:-16550220 Msa0720520:CDS
GGTAATTATTCAGGTAACTA+AGG 0.467491 5_1:-16550985 Msa0720520:intron
TGAAGGGACTGTGCTGTCTC+AGG 0.467824 5_1:-16550958 Msa0720520:CDS
TATCAATTTCCCACTGGAGT+TGG 0.469397 5_1:-16551529 Msa0720520:CDS
GATTTATCAGATATTTGGCC+GGG 0.469571 5_1:-16553293 Msa0720520:CDS
AATGGAACTTCATCTGACTC+TGG 0.470300 5_1:-16551353 Msa0720520:CDS
AAACCAAGGTGTGCCTTGTC+GGG 0.471326 5_1:+16550914 None:intergenic
CATAATTCACAGGTAGCACT+TGG 0.473153 5_1:+16550586 None:intergenic
ACCGTAGATCCTGCTCAATT+GGG 0.475265 5_1:-16553458 Msa0720520:CDS
AGCAGCCATTCTCCTAATGA+TGG 0.479019 5_1:-16552516 Msa0720520:intron
AATATTGAAAAGAGTGGGGC+TGG 0.480181 5_1:-16550774 Msa0720520:CDS
TTGCTGCATTCCATTTAAAG+AGG 0.480468 5_1:-16551593 Msa0720520:CDS
TCAAAAGAAGACCCTCAACT+AGG 0.481413 5_1:+16552538 None:intergenic
AGATATTTGGCCGGGGCCAA+TGG 0.483102 5_1:-16553285 Msa0720520:CDS
AATGAAAATATTGAAAAGAG+TGG 0.488221 5_1:-16550780 Msa0720520:CDS
ATGAAAATATTGAAAAGAGT+GGG 0.488375 5_1:-16550779 Msa0720520:CDS
TTTAGACATCACTGATGGCC+AGG 0.488436 5_1:-16550823 Msa0720520:CDS
ATCTCGCTGCGGAACATCAC+CGG 0.496487 5_1:+16553322 None:intergenic
CAAAAGAAGACCCTCAACTA+GGG 0.500648 5_1:+16552539 None:intergenic
GCCCAGCAGGAACTTGTTTG+CGG 0.500874 5_1:+16550658 None:intergenic
GCATACTGTGCCCCTAGTTG+AGG 0.501299 5_1:-16552550 Msa0720520:CDS
AGATACTATTTCAAGTGCAT+TGG 0.502024 5_1:-16551721 Msa0720520:CDS
GCATACACAGCAAGATCCAT+TGG 0.503857 5_1:+16553269 None:intergenic
ATCCTCAAAATAAAGCTGCC+AGG 0.504847 5_1:-16550232 Msa0720520:CDS
GATGTTCCGCAGCGAGATCA+TGG 0.509643 5_1:-16553317 Msa0720520:CDS
GCATAAGTTGGATGATTCAA+TGG 0.511129 5_1:-16551291 Msa0720520:CDS
CCGACTGGCATTGCAAAGCC+TGG 0.513078 5_1:-16550558 Msa0720520:CDS
ATCATCATCAGGCAAGTCTT+CGG 0.514591 5_1:-16550258 Msa0720520:CDS
ATGCTCCTGATGATGAGAAC+TGG 0.515150 5_1:-16553181 Msa0720520:CDS
AGTGGTAAAAGATCATCATC+AGG 0.517890 5_1:-16550269 Msa0720520:CDS
TCATCATCAGGAGCATAATC+CGG 0.519460 5_1:+16553188 None:intergenic
CCAGGCTTTGCAATGCCAGT+CGG 0.519525 5_1:+16550558 None:intergenic
AAAGCATGTGTTACCAAGGT+TGG 0.521069 5_1:+16552599 None:intergenic
TGTGAAACTCCAGCTGCCAC+AGG 0.523773 5_1:+16550050 None:intergenic
ACTCACCATCATTAGGAGAA+TGG 0.525951 5_1:+16552511 None:intergenic
GCCGCAAACAAGTTCCTGCT+GGG 0.528262 5_1:-16550659 Msa0720520:CDS
TGGAGGCAGTTTGGTTAGCA+AGG 0.528538 5_1:-16551920 Msa0720520:intron
TGAGTTTATGCAGTGTGCAT+TGG 0.528922 5_1:-16552001 Msa0720520:CDS
CAATCACCTGGAGTCTGAGA+AGG 0.530275 5_1:+16550423 None:intergenic
AAATCTTTATTTGAATGGAT+CGG 0.530296 5_1:-16550622 Msa0720520:CDS
TTAGGAATATGCCTGGTTCA+AGG 0.531245 5_1:-16550202 Msa0720520:CDS
CATACTGTGCCCCTAGTTGA+GGG 0.532476 5_1:-16552549 Msa0720520:CDS
GTCGGGCTGGCATAATTCAC+AGG 0.532843 5_1:+16550576 None:intergenic
AAAACGCTTACAATCGTCAA+TGG 0.544488 5_1:+16553098 None:intergenic
CTGGCATTGCAAAGCCTGGT+GGG 0.544914 5_1:-16550554 Msa0720520:CDS
AATCACCTGGAGTCTGAGAA+GGG 0.545095 5_1:+16550424 None:intergenic
ACCCAATTGAGCAGGATCTA+CGG 0.547215 5_1:+16553457 None:intergenic
TTGGTTAACCCCATATCCTC+AGG 0.548294 5_1:+16553371 None:intergenic
GGTAGCTGCCGACGGAAGCA+TGG 0.550788 5_1:+16550072 None:intergenic
TCTAGACTCAGGTCAATCAA+TGG 0.552026 5_1:+16551756 None:intergenic
GCCGGAAGCTTGGTATCCAC+CGG 0.552170 5_1:-16553207 Msa0720520:CDS
CAGGTAACTAAGGTATCTGA+AGG 0.552413 5_1:-16550975 Msa0720520:CDS
TATGGAGAAGCAGCTTATGT+TGG 0.552768 5_1:-16551631 Msa0720520:CDS
AGTGATGTCTAAAACAGACT+TGG 0.553424 5_1:+16550833 None:intergenic
GGAGCTGGGCTTGCCAACCT+TGG 0.555047 5_1:-16552612 Msa0720520:CDS
ATGGAACTTCATCTGACTCT+GGG 0.557189 5_1:-16551352 Msa0720520:CDS
ACAGAGAAGCAAGGAAGTAG+TGG 0.558974 5_1:-16550287 Msa0720520:CDS
ACCTGGAGTCTGAGAAGGGG+GGG 0.559134 5_1:+16550428 None:intergenic
TCCAGACACATGGCATAAGT+TGG 0.561978 5_1:-16551303 Msa0720520:CDS
TGCCACAGGGTAGCTGCCGA+CGG 0.563195 5_1:+16550064 None:intergenic
AGGACTGTTTCATCTGATAA+CGG 0.564213 5_1:+16552311 None:intergenic
TGATTTATCAGATATTTGGC+CGG 0.566794 5_1:-16553294 Msa0720520:CDS
TCAGCATATGAACTCCCACC+AGG 0.567820 5_1:+16550540 None:intergenic
GCAGGAACTTGTTTGCGGCA+AGG 0.568113 5_1:+16550663 None:intergenic
TCACCTGGAGTCTGAGAAGG+GGG 0.569077 5_1:+16550426 None:intergenic
TCCTCAAAATAAAGCTGCCA+GGG 0.569220 5_1:-16550231 Msa0720520:CDS
AAATTATCCACAAATACCAC+AGG 0.569292 5_1:+16552262 None:intergenic
AAAGAAATACCCAATTGAGC+AGG 0.570044 5_1:+16553449 None:intergenic
ACAAATAGAAATCGCGGACA+AGG 0.573534 5_1:-16549870 Msa0720520:three_prime_UTR
ATTGCGAAGAAAGTCTTTGG+AGG 0.579484 5_1:-16551937 Msa0720520:CDS
CACGGACATAAACTCATCGT+TGG 0.580359 5_1:+16553352 None:intergenic
ACAGCAAGATCCATTGGCCC+CGG 0.580619 5_1:+16553275 None:intergenic
CATATTCCTAACATATGCCC+TGG 0.580915 5_1:+16550214 None:intergenic
GCATATGTTAGGAATATGCC+TGG 0.584399 5_1:-16550209 Msa0720520:CDS
CAAGCATATCAATTTCCCAC+TGG 0.585157 5_1:-16551535 Msa0720520:CDS
TTCGCACTGCTCCAGACACA+TGG 0.586738 5_1:-16551313 Msa0720520:CDS
TTTCTGAGCCTGAGGATATG+GGG 0.586760 5_1:-16553379 Msa0720520:CDS
AATGCACCTCTCCTTGAACC+AGG 0.588351 5_1:+16550191 None:intergenic
TGCATTGGAGTCTTTCACAA+TGG 0.593024 5_1:-16551706 Msa0720520:CDS
TGAAAATATTGAAAAGAGTG+GGG 0.594431 5_1:-16550778 Msa0720520:CDS
AACTCTTCCAGTAGACTGCA+AGG 0.596809 5_1:+16552291 None:intergenic
AACATCACCGGTGTGCATCA+CGG 0.599073 5_1:+16553334 None:intergenic
GTGATGTCTAAAACAGACTT+GGG 0.604740 5_1:+16550834 None:intergenic
ATTGGAGTCTTTCACAATGG+TGG 0.605716 5_1:-16551703 Msa0720520:CDS
ATGCCCGACAAGGCACACCT+TGG 0.606806 5_1:-16550917 Msa0720520:CDS
GGATCTTGCTGTGTATGCTC+CGG 0.610441 5_1:-16553264 Msa0720520:CDS
TGATGCTAAGATTAAATGTG+AGG 0.612633 5_1:-16551673 Msa0720520:CDS
ATCACCTGGAGTCTGAGAAG+GGG 0.616978 5_1:+16550425 None:intergenic
TGAGAAAGCATGTGTTACCA+AGG 0.617381 5_1:+16552595 None:intergenic
ATTTATCAGATATTTGGCCG+GGG 0.621370 5_1:-16553292 Msa0720520:CDS
GGTGAAAATACCTTTATCTG+TGG 0.622911 5_1:-16549978 Msa0720520:three_prime_UTR
GCTCATTAGTGTCAGAAGAG+TGG 0.628120 5_1:+16551778 None:intergenic
AAATCAGGCCATGCTTCCGT+CGG 0.629660 5_1:-16550080 Msa0720520:CDS
GTGAAACTCCAGCTGCCACA+GGG 0.630296 5_1:+16550051 None:intergenic
ATCCTACTCAGATTCAAGCA+TGG 0.632686 5_1:+16550867 None:intergenic
AATATGCCTGGTTCAAGGAG+AGG 0.638632 5_1:-16550197 Msa0720520:CDS
GGTTGCAAATCCAACTCCAG+TGG 0.642634 5_1:+16551519 None:intergenic
TTCCATGCTTGAATCTGAGT+AGG 0.644297 5_1:-16550869 Msa0720520:CDS
ATAATTCACAGGTAGCACTT+GGG 0.648379 5_1:+16550587 None:intergenic
AAAAGAAGACCCTCAACTAG+GGG 0.651897 5_1:+16552540 None:intergenic
TGTCGGAGTTTCTGAGCCTG+AGG 0.652877 5_1:-16553387 Msa0720520:CDS
ACTACATACAATTTGGCTAG+AGG 0.656761 5_1:+16549935 None:intergenic
AAAGACCGATTCATCCAAGT+CGG 0.658745 5_1:-16550129 Msa0720520:CDS
TTCTCATGCATAATGGAACA+GGG 0.661403 5_1:+16549898 None:intergenic
ACAGATACAAATAGAAATCG+CGG 0.661812 5_1:-16549876 Msa0720520:three_prime_UTR
TTCCGTCGGCAGCTACCCTG+TGG 0.662854 5_1:-16550066 Msa0720520:CDS
ACTGGCATTGCAAAGCCTGG+TGG 0.663624 5_1:-16550555 Msa0720520:CDS
GTTGCAAATCCAACTCCAGT+GGG 0.671853 5_1:+16551520 None:intergenic
AGGTAACTAAGGTATCTGAA+GGG 0.674479 5_1:-16550974 Msa0720520:CDS
TGTTGTTCTTGTTCACCACG+TGG 0.675934 5_1:+16553527 None:intergenic
CACCTGGAGTCTGAGAAGGG+GGG 0.678451 5_1:+16550427 None:intergenic
AATTATCCACAAATACCACA+GGG 0.680495 5_1:+16552263 None:intergenic
TCCGCAAAACTTGAAAACCA+AGG 0.687758 5_1:+16550900 None:intergenic
TCATCAGGAGCATAATCCGG+TGG 0.690832 5_1:+16553191 None:intergenic
TTTATGTCCGTGATGCACAC+CGG 0.691794 5_1:-16553341 Msa0720520:CDS
GATGCTAAGATTAAATGTGA+GGG 0.695012 5_1:-16551672 Msa0720520:CDS
GGTGGATACCAAGCTTCCGG+CGG 0.695616 5_1:+16553209 None:intergenic
CATTTGAAGACAGAGAAGCA+AGG 0.707732 5_1:-16550296 Msa0720520:CDS
TGGAAGAGTTATATCCCCTG+TGG 0.712097 5_1:-16552278 Msa0720520:CDS
AAATCACCATGATCTCGCTG+CGG 0.730412 5_1:+16553311 None:intergenic
TCTGATTCAAATTCACCACG+TGG 0.785823 5_1:-16553542 Msa0720520:CDS
ATTATCCACAAATACCACAG+GGG 0.787360 5_1:+16552264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTGATTTAAATGTATA+TGG - chr5_1:16550994-16551013 Msa0720520:intron 15.0%
!! TAGAACCTTTAATTAATATA+AGG - chr5_1:16551357-16551376 Msa0720520:CDS 15.0%
!! ACTAACCTTATATTAATTAA+AGG + chr5_1:16551365-16551384 None:intergenic 15.0%
!!! CATTGAAATTTTGAAAGTTA+GGG + chr5_1:16549908-16549927 None:intergenic 20.0%
!!! ACATTGAAATTTTGAAAGTT+AGG + chr5_1:16549909-16549928 None:intergenic 20.0%
!! AATTACTAATACAAAATCAC+AGG + chr5_1:16550705-16550724 None:intergenic 20.0%
!! GCACAAACAATTAATATTAA+AGG + chr5_1:16550781-16550800 None:intergenic 20.0%
!!! TTTTCATTCAAAATAGTGTA+AGG + chr5_1:16551975-16551994 None:intergenic 20.0%
!! ACATGTTTAATGATAAAAGT+TGG - chr5_1:16552255-16552274 Msa0720520:intron 20.0%
!! TTTATTTAACACGTAATATC+TGG - chr5_1:16552464-16552483 Msa0720520:intron 20.0%
!! AAATAGAAAATGATTTATCC+TGG + chr5_1:16552668-16552687 None:intergenic 20.0%
!! AATGAAAATATTGAAAAGAG+TGG - chr5_1:16552690-16552709 Msa0720520:intron 20.0%
!! ATGAAAATATTGAAAAGAGT+GGG - chr5_1:16552691-16552710 Msa0720520:intron 20.0%
!!! TAAAGATTTTTCTGATTTGA+TGG + chr5_1:16552837-16552856 None:intergenic 20.0%
!!! CAGAAAAATCTTTATTTGAA+TGG - chr5_1:16552843-16552862 Msa0720520:intron 20.0%
!!! AAATCTTTATTTGAATGGAT+CGG - chr5_1:16552848-16552867 Msa0720520:intron 20.0%
! GAAGAAATGAAATTCTTTGT+TGG + chr5_1:16549841-16549860 None:intergenic 25.0%
!!! AATTTTGAAAGTTAGGGTTT+CGG + chr5_1:16549902-16549921 None:intergenic 25.0%
! ATGGTGATTTATCAGATATT+TGG - chr5_1:16550172-16550191 Msa0720520:CDS 25.0%
! ATTTAGATCAAATATGTGTC+TGG + chr5_1:16550605-16550624 None:intergenic 25.0%
!! ATTAATATTAAAGGTGTGTC+TGG + chr5_1:16550772-16550791 None:intergenic 25.0%
! ATTAATTGTTTGTGCAACAT+AGG - chr5_1:16550786-16550805 Msa0720520:CDS 25.0%
!!! AAGATTTTGACCTTTAGATT+GGG - chr5_1:16551097-16551116 Msa0720520:intron 25.0%
! TATCCTTAATACCATATTCA+TGG - chr5_1:16551237-16551256 Msa0720520:intron 25.0%
! TTTCCATGAATATGGTATTA+AGG + chr5_1:16551243-16551262 None:intergenic 25.0%
!! TTGCATATCTTTTCAATGAA+AGG - chr5_1:16551268-16551287 Msa0720520:intron 25.0%
! TAGAATTGTCTGTCATTAAT+TGG - chr5_1:16551294-16551313 Msa0720520:CDS 25.0%
! AGTAGTGAGTGTTATATTAT+TGG - chr5_1:16551323-16551342 Msa0720520:CDS 25.0%
!! TTAAAGGTTCTATATTTTGC+AGG + chr5_1:16551349-16551368 None:intergenic 25.0%
!!! AGGTTAGTTTCTTTTACTTT+TGG - chr5_1:16551377-16551396 Msa0720520:CDS 25.0%
!!! TTAGTTTCTTTTACTTTTGG+TGG - chr5_1:16551380-16551399 Msa0720520:CDS 25.0%
!!! GACATGTTTTTCAAATTTTG+AGG - chr5_1:16551502-16551521 Msa0720520:CDS 25.0%
!!! ATGTTTTTCAAATTTTGAGG+AGG - chr5_1:16551505-16551524 Msa0720520:CDS 25.0%
! GAAGCTGAAAAATAAATACA+AGG + chr5_1:16551656-16551675 None:intergenic 25.0%
! TCAGTATAAAGAATTCACAA+GGG + chr5_1:16552038-16552057 None:intergenic 25.0%
!!! TTTCTTCAAGATTTTTTCCT+AGG - chr5_1:16552310-16552329 Msa0720520:CDS 25.0%
! AGTTTGCAAAAACAGTAAAA+TGG + chr5_1:16552363-16552382 None:intergenic 25.0%
!! TGTTTTCTTTGCATATAGTT+AGG - chr5_1:16552401-16552420 Msa0720520:intron 25.0%
!! GTTTTCTTTGCATATAGTTA+GGG - chr5_1:16552402-16552421 Msa0720520:intron 25.0%
! GGCTTATTTAATAATTGCAT+TGG - chr5_1:16552423-16552442 Msa0720520:intron 25.0%
! GTAATATCTGGTAATTATTC+AGG - chr5_1:16552476-16552495 Msa0720520:intron 25.0%
! TGAAAATATTGAAAAGAGTG+GGG - chr5_1:16552692-16552711 Msa0720520:intron 25.0%
!! TCAATAACATCATCATTTTC+AGG + chr5_1:16552780-16552799 None:intergenic 25.0%
!! AAAAAGTATATTCAATCACC+TGG + chr5_1:16553062-16553081 None:intergenic 25.0%
!!! GGTGATTGAATATACTTTTT+TGG - chr5_1:16553062-16553081 Msa0720520:intron 25.0%
!!! TCTTTTTTCTCTTGTTTTCT+GGG + chr5_1:16553326-16553345 None:intergenic 25.0%
!! ATTGAATAGTCTTTGACTTT+AGG - chr5_1:16553442-16553461 Msa0720520:CDS 25.0%
ATTGAGAGAAAGAGAAGTTT+AGG + chr5_1:16549869-16549888 None:intergenic 30.0%
TGATTTATCAGATATTTGGC+CGG - chr5_1:16550176-16550195 Msa0720520:CDS 30.0%
AAAATCCGAGTAGAATTAAC+TGG + chr5_1:16550234-16550253 None:intergenic 30.0%
! AGTTAATTCTACTCGGATTT+TGG - chr5_1:16550233-16550252 Msa0720520:CDS 30.0%
! GAACTGGTTGTATGATTTTA+AGG - chr5_1:16550305-16550324 Msa0720520:CDS 30.0%
!! TGATTTTAAGGAATCCTTGA+AGG - chr5_1:16550317-16550336 Msa0720520:CDS 30.0%
ATCATAAACTTCATCCTTCA+AGG + chr5_1:16550334-16550353 None:intergenic 30.0%
ATCCAGATTAAATGTGTGTT+TGG + chr5_1:16550680-16550699 None:intergenic 30.0%
!! CAACATAGGTTTTGTCTTTT+TGG - chr5_1:16550800-16550819 Msa0720520:CDS 30.0%
!! TTGACGATCTTTTGTTTAAC+AGG - chr5_1:16550836-16550855 Msa0720520:CDS 30.0%
!! TGACGATCTTTTGTTTAACA+GGG - chr5_1:16550837-16550856 Msa0720520:CDS 30.0%
!! GAAGATTTTGACCTTTAGAT+TGG - chr5_1:16551096-16551115 Msa0720520:intron 30.0%
AATTATCCACAAATACCACA+GGG + chr5_1:16551210-16551229 None:intergenic 30.0%
AAATTATCCACAAATACCAC+AGG + chr5_1:16551211-16551230 None:intergenic 30.0%
CAAGAAAGTTTCCATGAATA+TGG + chr5_1:16551251-16551270 None:intergenic 30.0%
GAAAATTCTGATACAAGTCA+TGG + chr5_1:16551413-16551432 None:intergenic 30.0%
!! TTTTAGAAGACATATGCAAG+AGG - chr5_1:16551439-16551458 Msa0720520:intron 30.0%
AATATTGCGAAGAAAGTCTT+TGG - chr5_1:16551530-16551549 Msa0720520:CDS 30.0%
CATACCATTCTGAATCAAAA+AGG + chr5_1:16551613-16551632 None:intergenic 30.0%
!!! CATTTTCTATTTCTAGACTC+AGG + chr5_1:16551728-16551747 None:intergenic 30.0%
! AGATACTATTTCAAGTGCAT+TGG - chr5_1:16551749-16551768 Msa0720520:CDS 30.0%
TGATGCTAAGATTAAATGTG+AGG - chr5_1:16551797-16551816 Msa0720520:CDS 30.0%
GATGCTAAGATTAAATGTGA+GGG - chr5_1:16551798-16551817 Msa0720520:CDS 30.0%
CTGTCTTAAACCTCTTTAAA+TGG + chr5_1:16551890-16551909 None:intergenic 30.0%
GTCAGTATAAAGAATTCACA+AGG + chr5_1:16552039-16552058 None:intergenic 30.0%
TGCCTGTCAACATAAAAAAT+GGG + chr5_1:16552061-16552080 None:intergenic 30.0%
ATGCCTGTCAACATAAAAAA+TGG + chr5_1:16552062-16552081 None:intergenic 30.0%
TCTGCTATAATTGTTCTACT+TGG - chr5_1:16552207-16552226 Msa0720520:intron 30.0%
AAAAGTTGGCTGTAACTTTA+TGG - chr5_1:16552269-16552288 Msa0720520:CDS 30.0%
GGTAATTATTCAGGTAACTA+AGG - chr5_1:16552485-16552504 Msa0720520:intron 30.0%
AGACTTCTCAGAAAATTCTT+TGG + chr5_1:16552727-16552746 None:intergenic 30.0%
!!! AGACTGCATTTGTTTTTATC+AGG + chr5_1:16552966-16552985 None:intergenic 30.0%
TGATAAAAACAAATGCAGTC+TGG - chr5_1:16552965-16552984 Msa0720520:intron 30.0%
TTGTTCAATAACCATTGAGA+TGG - chr5_1:16553116-16553135 Msa0720520:CDS 30.0%
!!! CTTTTTTCTCTTGTTTTCTG+GGG + chr5_1:16553325-16553344 None:intergenic 30.0%
!!! GTCTTTTTTCTCTTGTTTTC+TGG + chr5_1:16553327-16553346 None:intergenic 30.0%
AACAAAAAGACCACAGATAA+AGG + chr5_1:16553505-16553524 None:intergenic 30.0%
TGTCATGACTACATACAATT+TGG + chr5_1:16553545-16553564 None:intergenic 30.0%
ACAGATACAAATAGAAATCG+CGG - chr5_1:16553594-16553613 Msa0720520:five_prime_UTR 30.0%
AAAGAAATACCCAATTGAGC+AGG + chr5_1:16550024-16550043 None:intergenic 35.0%
TGCTCAATTGGGTATTTCTT+TGG - chr5_1:16550023-16550042 Msa0720520:three_prime_UTR 35.0%
GACGTTTATGTTGATGTTGT+CGG - chr5_1:16550066-16550085 Msa0720520:CDS 35.0%
GATTTATCAGATATTTGGCC+GGG - chr5_1:16550177-16550196 Msa0720520:CDS 35.0%
ATTTATCAGATATTTGGCCG+GGG - chr5_1:16550178-16550197 Msa0720520:CDS 35.0%
AAAACGCTTACAATCGTCAA+TGG + chr5_1:16550375-16550394 None:intergenic 35.0%
AATTCAGATCAATTGTGCGT+CGG + chr5_1:16550525-16550544 None:intergenic 35.0%
CTGAATTGTATGTGCTACAT+AGG - chr5_1:16550538-16550557 Msa0720520:CDS 35.0%
CACCAAACACACATTTAATC+TGG - chr5_1:16550675-16550694 Msa0720520:CDS 35.0%
ACCTTTAGATTGGGAATTTC+AGG - chr5_1:16551106-16551125 Msa0720520:intron 35.0%
ACCTGAAATTCCCAATCTAA+AGG + chr5_1:16551110-16551129 None:intergenic 35.0%
! TTGGGAATTTCAGGTCATAA+TGG - chr5_1:16551115-16551134 Msa0720520:intron 35.0%
! TGGGAATTTCAGGTCATAAT+GGG - chr5_1:16551116-16551135 Msa0720520:intron 35.0%
AGGACTGTTTCATCTGATAA+CGG + chr5_1:16551162-16551181 None:intergenic 35.0%
ATTATCCACAAATACCACAG+GGG + chr5_1:16551209-16551228 None:intergenic 35.0%
!! GTCTCCTTTTTGATTCAGAA+TGG - chr5_1:16551606-16551625 Msa0720520:CDS 35.0%
AAATGGAATGCAGCAATTGA+AGG + chr5_1:16551873-16551892 None:intergenic 35.0%
TTGCTGCATTCCATTTAAAG+AGG - chr5_1:16551877-16551896 Msa0720520:intron 35.0%
!!! GACCCATTTTTTATGTTGAC+AGG - chr5_1:16552056-16552075 Msa0720520:intron 35.0%
TATGCAGTTGTTGTGCATAA+TGG - chr5_1:16552099-16552118 Msa0720520:intron 35.0%
! GCATAAGTTGGATGATTCAA+TGG - chr5_1:16552179-16552198 Msa0720520:intron 35.0%
AGTTTCAAGCTATGAAGACT+GGG + chr5_1:16552338-16552357 None:intergenic 35.0%
TAGTTTCAAGCTATGAAGAC+TGG + chr5_1:16552339-16552358 None:intergenic 35.0%
! AGGTAACTAAGGTATCTGAA+GGG - chr5_1:16552496-16552515 Msa0720520:intron 35.0%
!! ATATTGTTTTATGCCCGACA+AGG - chr5_1:16552543-16552562 Msa0720520:CDS 35.0%
!!! ACCTTGGTTTTCAAGTTTTG+CGG - chr5_1:16552569-16552588 Msa0720520:CDS 35.0%
! GTGATGTCTAAAACAGACTT+GGG + chr5_1:16552639-16552658 None:intergenic 35.0%
! AGTGATGTCTAAAACAGACT+TGG + chr5_1:16552640-16552659 None:intergenic 35.0%
!! TCTGTTTTAGACATCACTGA+TGG - chr5_1:16552642-16552661 Msa0720520:intron 35.0%
ATAATTCACAGGTAGCACTT+GGG + chr5_1:16552886-16552905 None:intergenic 35.0%
AGTGGTAAAAGATCATCATC+AGG - chr5_1:16553201-16553220 Msa0720520:CDS 35.0%
! AGTCTTTGACTTTAGGCATT+TGG - chr5_1:16553449-16553468 Msa0720520:CDS 35.0%
GGTGAAAATACCTTTATCTG+TGG - chr5_1:16553492-16553511 Msa0720520:CDS 35.0%
! TACAATTTGGCTAGAGGTAT+TGG + chr5_1:16553532-16553551 None:intergenic 35.0%
ACTACATACAATTTGGCTAG+AGG + chr5_1:16553538-16553557 None:intergenic 35.0%
! TTCTCATGCATAATGGAACA+GGG + chr5_1:16553575-16553594 None:intergenic 35.0%
GTATCTGTTCTCATGCATAA+TGG + chr5_1:16553582-16553601 None:intergenic 35.0%
TCTGATTCAAATTCACCACG+TGG - chr5_1:16549928-16549947 Msa0720520:three_prime_UTR 40.0%
CGAGTAGAATTAACTGGAAC+CGG + chr5_1:16550228-16550247 None:intergenic 40.0%
CGGTTCCAGTTAATTCTACT+CGG - chr5_1:16550226-16550245 Msa0720520:CDS 40.0%
! TCATCATCAGGAGCATAATC+CGG + chr5_1:16550285-16550304 None:intergenic 40.0%
TACAACCAGTTCTCATCATC+AGG + chr5_1:16550297-16550316 None:intergenic 40.0%
ATTCAGATCAATTGTGCGTC+GGG + chr5_1:16550524-16550543 None:intergenic 40.0%
CTAAATTGTCTGTGCTACAG+AGG - chr5_1:16550619-16550638 Msa0720520:CDS 40.0%
!! TCTTTTGTTTAACAGGGAGC+TGG - chr5_1:16550843-16550862 Msa0720520:CDS 40.0%
!! CTTTTGTTTAACAGGGAGCT+GGG - chr5_1:16550844-16550863 Msa0720520:CDS 40.0%
AAAGCATGTGTTACCAAGGT+TGG + chr5_1:16550874-16550893 None:intergenic 40.0%
! TGAGAAAGCATGTGTTACCA+AGG + chr5_1:16550878-16550897 None:intergenic 40.0%
AAAAGAAGACCCTCAACTAG+GGG + chr5_1:16550933-16550952 None:intergenic 40.0%
CAAAAGAAGACCCTCAACTA+GGG + chr5_1:16550934-16550953 None:intergenic 40.0%
TCAAAAGAAGACCCTCAACT+AGG + chr5_1:16550935-16550954 None:intergenic 40.0%
ACTCACCATCATTAGGAGAA+TGG + chr5_1:16550962-16550981 None:intergenic 40.0%
! TCCTAATGATGGTGAGTAAG+AGG - chr5_1:16550965-16550984 Msa0720520:CDS 40.0%
TCCTCTTACTCACCATCATT+AGG + chr5_1:16550969-16550988 None:intergenic 40.0%
TATATCCCCTGTGGTATTTG+TGG - chr5_1:16551201-16551220 Msa0720520:intron 40.0%
TGAGTTTATGCAGTGTGCAT+TGG - chr5_1:16551469-16551488 Msa0720520:intron 40.0%
ATTGCGAAGAAAGTCTTTGG+AGG - chr5_1:16551533-16551552 Msa0720520:CDS 40.0%
TCTAGACTCAGGTCAATCAA+TGG + chr5_1:16551717-16551736 None:intergenic 40.0%
TGCATTGGAGTCTTTCACAA+TGG - chr5_1:16551764-16551783 Msa0720520:CDS 40.0%
ATTGGAGTCTTTCACAATGG+TGG - chr5_1:16551767-16551786 Msa0720520:CDS 40.0%
!! CTGCAATGAAGAAGTGTCTA+TGG - chr5_1:16551821-16551840 Msa0720520:intron 40.0%
TATGGAGAAGCAGCTTATGT+TGG - chr5_1:16551839-16551858 Msa0720520:intron 40.0%
CAAGCATATCAATTTCCCAC+TGG - chr5_1:16551935-16551954 Msa0720520:CDS 40.0%
TATCAATTTCCCACTGGAGT+TGG - chr5_1:16551941-16551960 Msa0720520:CDS 40.0%
AATGGAACTTCATCTGACTC+TGG - chr5_1:16552117-16552136 Msa0720520:intron 40.0%
ATGGAACTTCATCTGACTCT+GGG - chr5_1:16552118-16552137 Msa0720520:intron 40.0%
! CAGGTAACTAAGGTATCTGA+AGG - chr5_1:16552495-16552514 Msa0720520:intron 40.0%
TCCGCAAAACTTGAAAACCA+AGG + chr5_1:16552573-16552592 None:intergenic 40.0%
TTCCATGCTTGAATCTGAGT+AGG - chr5_1:16552601-16552620 Msa0720520:CDS 40.0%
ATCCTACTCAGATTCAAGCA+TGG + chr5_1:16552606-16552625 None:intergenic 40.0%
AATATTGAAAAGAGTGGGGC+TGG - chr5_1:16552696-16552715 Msa0720520:intron 40.0%
CATAATTCACAGGTAGCACT+TGG + chr5_1:16552887-16552906 None:intergenic 40.0%
! ACCATTGAGATGGATTTGTC+AGG - chr5_1:16553126-16553145 Msa0720520:CDS 40.0%
ACCTGACAAATCCATCTCAA+TGG + chr5_1:16553130-16553149 None:intergenic 40.0%
CATTTGAAGACAGAGAAGCA+AGG - chr5_1:16553174-16553193 Msa0720520:CDS 40.0%
ATCATCATCAGGCAAGTCTT+CGG - chr5_1:16553212-16553231 Msa0720520:CDS 40.0%
ATCCTCAAAATAAAGCTGCC+AGG - chr5_1:16553238-16553257 Msa0720520:CDS 40.0%
TCCTCAAAATAAAGCTGCCA+GGG - chr5_1:16553239-16553258 Msa0720520:CDS 40.0%
CATATTCCTAACATATGCCC+TGG + chr5_1:16553259-16553278 None:intergenic 40.0%
GCATATGTTAGGAATATGCC+TGG - chr5_1:16553261-16553280 Msa0720520:CDS 40.0%
TTAGGAATATGCCTGGTTCA+AGG - chr5_1:16553268-16553287 Msa0720520:CDS 40.0%
!! TTCAAGGAGAGGTGCATTTT+TGG - chr5_1:16553284-16553303 Msa0720520:CDS 40.0%
!! GGTGCATTTTTGGACTTACT+TGG - chr5_1:16553294-16553313 Msa0720520:CDS 40.0%
!! GCATTTTTGGACTTACTTGG+TGG - chr5_1:16553297-16553316 Msa0720520:CDS 40.0%
! AAAGACCGATTCATCCAAGT+CGG - chr5_1:16553341-16553360 Msa0720520:CDS 40.0%
!! TTTGTCCGACTTGGATGAAT+CGG + chr5_1:16553349-16553368 None:intergenic 40.0%
!!! TGGCTGTGTACTTGGTTTTT+TGG - chr5_1:16553469-16553488 Msa0720520:CDS 40.0%
!!! GGCTGTGTACTTGGTTTTTT+GGG - chr5_1:16553470-16553489 Msa0720520:CDS 40.0%
!!! GCTGTGTACTTGGTTTTTTG+GGG - chr5_1:16553471-16553490 Msa0720520:CDS 40.0%
! GTTCTCATGCATAATGGAAC+AGG + chr5_1:16553576-16553595 None:intergenic 40.0%
ACAAATAGAAATCGCGGACA+AGG - chr5_1:16553600-16553619 Msa0720520:five_prime_UTR 40.0%
TGTTGTTCTTGTTCACCACG+TGG + chr5_1:16549946-16549965 None:intergenic 45.0%
AACCGTAGATCCTGCTCAAT+TGG - chr5_1:16550011-16550030 Msa0720520:three_prime_UTR 45.0%
ACCGTAGATCCTGCTCAATT+GGG - chr5_1:16550012-16550031 Msa0720520:three_prime_UTR 45.0%
ACCCAATTGAGCAGGATCTA+CGG + chr5_1:16550016-16550035 None:intergenic 45.0%
AGTTTCTGAGCCTGAGGATA+TGG - chr5_1:16550089-16550108 Msa0720520:CDS 45.0%
GTTTCTGAGCCTGAGGATAT+GGG - chr5_1:16550090-16550109 Msa0720520:CDS 45.0%
TTTCTGAGCCTGAGGATATG+GGG - chr5_1:16550091-16550110 Msa0720520:CDS 45.0%
TTGGTTAACCCCATATCCTC+AGG + chr5_1:16550102-16550121 None:intergenic 45.0%
CACGGACATAAACTCATCGT+TGG + chr5_1:16550121-16550140 None:intergenic 45.0%
TTTATGTCCGTGATGCACAC+CGG - chr5_1:16550129-16550148 Msa0720520:CDS 45.0%
AAATCACCATGATCTCGCTG+CGG + chr5_1:16550162-16550181 None:intergenic 45.0%
GCATACACAGCAAGATCCAT+TGG + chr5_1:16550204-16550223 None:intergenic 45.0%
! ATGCTCCTGATGATGAGAAC+TGG - chr5_1:16550289-16550308 Msa0720520:CDS 45.0%
ATTGTCCACCGACACATGTT+AGG - chr5_1:16550652-16550671 Msa0720520:CDS 45.0%
!! GTTTGGTGCCTAACATGTGT+CGG + chr5_1:16550663-16550682 None:intergenic 45.0%
AGCAGCCATTCTCCTAATGA+TGG - chr5_1:16550954-16550973 Msa0720520:CDS 45.0%
AAACAGTCCTTGCAGTCTAC+TGG - chr5_1:16551172-16551191 Msa0720520:intron 45.0%
AACTCTTCCAGTAGACTGCA+AGG + chr5_1:16551182-16551201 None:intergenic 45.0%
TGGAAGAGTTATATCCCCTG+TGG - chr5_1:16551192-16551211 Msa0720520:intron 45.0%
! GAAAGTCTTTGGAGGCAGTT+TGG - chr5_1:16551541-16551560 Msa0720520:CDS 45.0%
GCTCATTAGTGTCAGAAGAG+TGG + chr5_1:16551695-16551714 None:intergenic 45.0%
GTTGCAAATCCAACTCCAGT+GGG + chr5_1:16551953-16551972 None:intergenic 45.0%
TCCAGACACATGGCATAAGT+TGG - chr5_1:16552167-16552186 Msa0720520:intron 45.0%
TCCAACTTATGCCATGTGTC+TGG + chr5_1:16552171-16552190 None:intergenic 45.0%
AAAACCAAGGTGTGCCTTGT+CGG + chr5_1:16552560-16552579 None:intergenic 45.0%
TTTAGACATCACTGATGGCC+AGG - chr5_1:16552647-16552666 Msa0720520:intron 45.0%
AATCACCTGGAGTCTGAGAA+GGG + chr5_1:16553049-16553068 None:intergenic 45.0%
TCAAATGATCGCGTGAGATC+TGG + chr5_1:16553161-16553180 None:intergenic 45.0%
ACAGAGAAGCAAGGAAGTAG+TGG - chr5_1:16553183-16553202 Msa0720520:CDS 45.0%
AATATGCCTGGTTCAAGGAG+AGG - chr5_1:16553273-16553292 Msa0720520:CDS 45.0%
TAGGCATTTGGCTGTGTACT+TGG - chr5_1:16553461-16553480 Msa0720520:CDS 45.0%
AACATCACCGGTGTGCATCA+CGG + chr5_1:16550139-16550158 None:intergenic 50.0%
! GGATCTTGCTGTGTATGCTC+CGG - chr5_1:16550206-16550225 Msa0720520:CDS 50.0%
! TCATCAGGAGCATAATCCGG+TGG + chr5_1:16550282-16550301 None:intergenic 50.0%
CATACTGTGCCCCTAGTTGA+GGG - chr5_1:16550921-16550940 Msa0720520:CDS 50.0%
TGGAGGCAGTTTGGTTAGCA+AGG - chr5_1:16551550-16551569 Msa0720520:CDS 50.0%
GGTTGCAAATCCAACTCCAG+TGG + chr5_1:16551954-16551973 None:intergenic 50.0%
AAACCAAGGTGTGCCTTGTC+GGG + chr5_1:16552559-16552578 None:intergenic 50.0%
! TTCTGATTTGATGGCCCAGC+AGG + chr5_1:16552828-16552847 None:intergenic 50.0%
TCAGCATATGAACTCCCACC+AGG + chr5_1:16552933-16552952 None:intergenic 50.0%
ATCACCTGGAGTCTGAGAAG+GGG + chr5_1:16553048-16553067 None:intergenic 50.0%
CAATCACCTGGAGTCTGAGA+AGG + chr5_1:16553050-16553069 None:intergenic 50.0%
!!! GCCCTGGCAGCTTTATTTTG+AGG + chr5_1:16553243-16553262 None:intergenic 50.0%
AAGCTGCCAGGGCATATGTT+AGG - chr5_1:16553250-16553269 Msa0720520:CDS 50.0%
AATGCACCTCTCCTTGAACC+AGG + chr5_1:16553282-16553301 None:intergenic 50.0%
AGCAGTGCTCGCAAGAAATC+AGG - chr5_1:16553375-16553394 Msa0720520:CDS 50.0%
AAATCAGGCCATGCTTCCGT+CGG - chr5_1:16553390-16553409 Msa0720520:CDS 50.0%
TGTCGGAGTTTCTGAGCCTG+AGG - chr5_1:16550083-16550102 Msa0720520:CDS 55.0%
ATCTCGCTGCGGAACATCAC+CGG + chr5_1:16550151-16550170 None:intergenic 55.0%
GATGTTCCGCAGCGAGATCA+TGG - chr5_1:16550153-16550172 Msa0720520:CDS 55.0%
AGATATTTGGCCGGGGCCAA+TGG - chr5_1:16550185-16550204 Msa0720520:CDS 55.0%
ACAGCAAGATCCATTGGCCC+CGG + chr5_1:16550198-16550217 None:intergenic 55.0%
TCCGGTGGATACCAAGCTTC+CGG + chr5_1:16550267-16550286 None:intergenic 55.0%
TGGTGCCTAACATGTGTCGG+TGG + chr5_1:16550660-16550679 None:intergenic 55.0%
GCATACTGTGCCCCTAGTTG+AGG - chr5_1:16550920-16550939 Msa0720520:CDS 55.0%
TTCGCACTGCTCCAGACACA+TGG - chr5_1:16552157-16552176 Msa0720520:intron 55.0%
GCTATGAAGACTGGGCTCCT+AGG + chr5_1:16552330-16552349 None:intergenic 55.0%
TGAAGGGACTGTGCTGTCTC+AGG - chr5_1:16552512-16552531 Msa0720520:intron 55.0%
! GCAGGAACTTGTTTGCGGCA+AGG + chr5_1:16552810-16552829 None:intergenic 55.0%
TGCCGCAAACAAGTTCCTGC+TGG - chr5_1:16552810-16552829 Msa0720520:intron 55.0%
GCCGCAAACAAGTTCCTGCT+GGG - chr5_1:16552811-16552830 Msa0720520:intron 55.0%
! GCCCAGCAGGAACTTGTTTG+CGG + chr5_1:16552815-16552834 None:intergenic 55.0%
!! GTCGGGCTGGCATAATTCAC+AGG + chr5_1:16552897-16552916 None:intergenic 55.0%
GTGAATTATGCCAGCCCGAC+TGG - chr5_1:16552897-16552916 Msa0720520:intron 55.0%
CAGGCTTTGCAATGCCAGTC+GGG + chr5_1:16552914-16552933 None:intergenic 55.0%
CCAGGCTTTGCAATGCCAGT+CGG + chr5_1:16552915-16552934 None:intergenic 55.0%
ACTGGCATTGCAAAGCCTGG+TGG - chr5_1:16552915-16552934 Msa0720520:intron 55.0%
CTGGCATTGCAAAGCCTGGT+GGG - chr5_1:16552916-16552935 Msa0720520:intron 55.0%
GGGGGAGTTAGGAGGAGTTA+AGG + chr5_1:16553027-16553046 None:intergenic 55.0%
TCACCTGGAGTCTGAGAAGG+GGG + chr5_1:16553047-16553066 None:intergenic 55.0%
GCTGCTGTCTTTGTCCGACT+TGG + chr5_1:16553358-16553377 None:intergenic 55.0%
GTGAAACTCCAGCTGCCACA+GGG + chr5_1:16553422-16553441 None:intergenic 55.0%
TGTGAAACTCCAGCTGCCAC+AGG + chr5_1:16553423-16553442 None:intergenic 55.0%
!!! TCGGATTTTGGCTGTGCCGC+CGG - chr5_1:16550245-16550264 Msa0720520:CDS 60.0%
GGTGGATACCAAGCTTCCGG+CGG + chr5_1:16550264-16550283 None:intergenic 60.0%
GCCGGAAGCTTGGTATCCAC+CGG - chr5_1:16550263-16550282 Msa0720520:CDS 60.0%
ATGCCCGACAAGGCACACCT+TGG - chr5_1:16552553-16552572 Msa0720520:CDS 60.0%
CTTTGCAATGCCAGTCGGGC+TGG + chr5_1:16552910-16552929 None:intergenic 60.0%
CCGACTGGCATTGCAAAGCC+TGG - chr5_1:16552912-16552931 Msa0720520:intron 60.0%
TGAGAAGGGGGGGAGTTAGG+AGG + chr5_1:16553035-16553054 None:intergenic 60.0%
GTCTGAGAAGGGGGGGAGTT+AGG + chr5_1:16553038-16553057 None:intergenic 60.0%
ACCTGGAGTCTGAGAAGGGG+GGG + chr5_1:16553045-16553064 None:intergenic 60.0%
CACCTGGAGTCTGAGAAGGG+GGG + chr5_1:16553046-16553065 None:intergenic 60.0%
GGAGCTGGGCTTGCCAACCT+TGG - chr5_1:16550858-16550877 Msa0720520:CDS 65.0%
TCCCCCCCTTCTCAGACTCC+AGG - chr5_1:16553041-16553060 Msa0720520:intron 65.0%
GGTAGCTGCCGACGGAAGCA+TGG + chr5_1:16553401-16553420 None:intergenic 65.0%
TTCCGTCGGCAGCTACCCTG+TGG - chr5_1:16553404-16553423 Msa0720520:CDS 65.0%
TGCCACAGGGTAGCTGCCGA+CGG + chr5_1:16553409-16553428 None:intergenic 65.0%
TGGCTGTGCCGCCGGAAGCT+TGG - chr5_1:16550253-16550272 Msa0720520:CDS 70.0%
GGCAGCTACCCTGTGGCAGC+TGG - chr5_1:16553411-16553430 Msa0720520:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 16549831 16553661 16549831 ID=Msa0720520;Name=Msa0720520
chr5_1 mRNA 16549831 16553661 16549831 ID=Msa0720520-mRNA-1;Parent=Msa0720520;Name=Msa0720520-mRNA-1;_AED=0.19;_eAED=0.19;_QI=94|0.85|0.87|1|1|1|8|215|721
chr5_1 exon 16549831 16550344 16549831 ID=Msa0720520-mRNA-1:exon:6296;Parent=Msa0720520-mRNA-1
chr5_1 exon 16550430 16550994 16550430 ID=Msa0720520-mRNA-1:exon:6295;Parent=Msa0720520-mRNA-1
chr5_1 exon 16551274 16551414 16551274 ID=Msa0720520-mRNA-1:exon:6294;Parent=Msa0720520-mRNA-1
chr5_1 exon 16551491 16551820 16551491 ID=Msa0720520-mRNA-1:exon:6293;Parent=Msa0720520-mRNA-1
chr5_1 exon 16551921 16552066 16551921 ID=Msa0720520-mRNA-1:exon:6292;Parent=Msa0720520-mRNA-1
chr5_1 exon 16552257 16552364 16552257 ID=Msa0720520-mRNA-1:exon:6291;Parent=Msa0720520-mRNA-1
chr5_1 exon 16552517 16552634 16552517 ID=Msa0720520-mRNA-1:exon:6290;Parent=Msa0720520-mRNA-1
chr5_1 exon 16553109 16553661 16553109 ID=Msa0720520-mRNA-1:exon:6289;Parent=Msa0720520-mRNA-1
chr5_1 five_prime_UTR 16553568 16553661 16553568 ID=Msa0720520-mRNA-1:five_prime_utr;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16553109 16553567 16553109 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16552517 16552634 16552517 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16552257 16552364 16552257 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16551921 16552066 16551921 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16551491 16551820 16551491 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16551274 16551414 16551274 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16550430 16550994 16550430 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 CDS 16550046 16550344 16550046 ID=Msa0720520-mRNA-1:cds;Parent=Msa0720520-mRNA-1
chr5_1 three_prime_UTR 16549831 16550045 16549831 ID=Msa0720520-mRNA-1:three_prime_utr;Parent=Msa0720520-mRNA-1
Gene Sequence

>Msa0720520

ATGTCTGATTCAAATTCACCACGTGGTGAACAAGAACAACAAGAGAATCAATTGAATTCATCTTCAACGATGAGTTCAATTTCTGAAACCGTAGATCCTGCTCAATTGGGTATTTCTTTGGCTGATCTTGATGATGACATTGACGTTTATGTTGATGTTGTCGGAGTTTCTGAGCCTGAGGATATGGGGTTAACCAACGATGAGTTTATGTCCGTGATGCACACCGGTGATGTTCCGCAGCGAGATCATGGTGATTTATCAGATATTTGGCCGGGGCCAATGGATCTTGCTGTGTATGCTCCGGTTCCAGTTAATTCTACTCGGATTTTGGCTGTGCCGCCGGAAGCTTGGTATCCACCGGATTATGCTCCTGATGATGAGAACTGGTTGTATGATTTTAAGGAATCCTTGAAGGATGAAGTTTATGATTCTGATTCTAAAGTTGTGCCATTGACGATTGGAGCTGGGCTTGCCAACCTTGGTAACACATGCTTTCTCAATTCAATTCTGCAATGCTTCACGCATACTGTGCCCCTAGTTGAGGGTCTTCTTTTGAGCAGCCATTCTCCTAATGATGGTCATAATGGGTTCTGTGTTATCTGTGCTTTCCGTTATCAGATGAAACAGTCCTTGCAGTCTACTGGAAGAGTTATATCCCCTGTGGTATTTGTGGATAATTTGAAGCAATTTTCTAATGATTTTAGAAGACATATGCAAGAGGATGCACATGAGTTTATGCAGTGTGCATTGGATAAACTTGAGACATGTTTTTCAAATTTTGAGGAGGATAATATTGCGAAGAAAGTCTTTGGAGGCAGTTTGGTTAGCAAGCTTCAGTGTTGTAACTGTAGCCACTCTTCTGACACTAATGAGCCATTGATTGACCTGAGTCTAGAAATAGAAAATGTAGATACTATTTCAAGTGCATTGGAGTCTTTCACAATGGTGGAAAAGATTGATGCTAAGATTAAATGTGAGGGCTGCAATGAAGAAGTGTCTATGGAGAAGCAGCTTATGTTGGATCAGACACCTTCAATTGCTGCATTCCATTTAAAGAGGTTTAAGACAGTTGAGAAGTCAGTTGAAAAGATTGACAAGCATATCAATTTCCCACTGGAGTTGGATTTGCAACCTTACACTATTTTGAATGAAAACAATGATGCATCATTGAAATATGATCTATATGCAGTTGTTGTGCATAATGGAACTTCATCTGACTCTGGGCATTATTTCTGCTTTGTTCGCACTGCTCCAGACACATGGCATAAGTTGGATGATTCAATGGTAGCATCTGCTATAATTGTAACTAAGGTATCTGAAGGGACTGTGCTGTCTCAGGAAGCATACATATTGTTTTATGCCCGACAAGGCACACCTTGGTTTTCAAGTTTTGCGGAATCTACAATTCCATGCTTGAATCTGAGTAGGATGAACACGTCTCCCAAGTCTGTTTTAGACATCACTGATGGCCAGGATAAATCATTTTCTATTTTAAATGAAAATATTGAAAAGAGTGGGGCTGGTAAATCCAAAGAATTTTCTGAGAAGTCTGATTATTCTTGTCAACAAAGTCGCAAGTTTCCTGAAAATGATGATGTTATTGATGCTTCTCCTTGCCGCAAACAAGTTCCTGCTGGGCCATCAAATCAGAAAAATCTTTATTTGAATGGATCGGAAGATATCAGCGCCCAAGTGCTACCTGTGAATTATGCCAGCCCGACTGGCATTGCAAAGCCTGGTGGGAGTTCATATGCTGAGAATGTTGCTCCTGATAAAAACAAATGCAGTCTGGAAGCTAATGATTTTATAGAGAATGATGTTTTCAATGCCTTAACTCCTCCTAACTCCCCCCCTTCTCAGACTCCAGGTAAGAGTTTCCAGATCTCACGCGATCATTTGAAGACAGAGAAGCAAGGAAGTAGTGGTAAAAGATCATCATCAGGCAAGTCTTCGGATAATCCTCAAAATAAAGCTGCCAGGGCATATGTTAGGAATATGCCTGGTTCAAGGAGAGGTGCATTTTTGGACTTACTTGGTGGTTCCCCAGAAAACAAGAGAAAAAAGACCGATTCATCCAAGTCGGACAAAGACAGCAGCAGTGCTCGCAAGAAATCAGGCCATGCTTCCGTCGGCAGCTACCCTGTGGCAGCTGGAGTTTCACATTGA
Protein sequence

>Msa0720520

MSDSNSPRGEQEQQENQLNSSSTMSSISETVDPAQLGISLADLDDDIDVYVDVVGVSEPEDMGLTNDEFMSVMHTGDVPQRDHGDLSDIWPGPMDLAVYAPVPVNSTRILAVPPEAWYPPDYAPDDENWLYDFKESLKDEVYDSDSKVVPLTIGAGLANLGNTCFLNSILQCFTHTVPLVEGLLLSSHSPNDGHNGFCVICAFRYQMKQSLQSTGRVISPVVFVDNLKQFSNDFRRHMQEDAHEFMQCALDKLETCFSNFEEDNIAKKVFGGSLVSKLQCCNCSHSSDTNEPLIDLSLEIENVDTISSALESFTMVEKIDAKIKCEGCNEEVSMEKQLMLDQTPSIAAFHLKRFKTVEKSVEKIDKHINFPLELDLQPYTILNENNDASLKYDLYAVVVHNGTSSDSGHYFCFVRTAPDTWHKLDDSMVASAIIVTKVSEGTVLSQEAYILFYARQGTPWFSSFAESTIPCLNLSRMNTSPKSVLDITDGQDKSFSILNENIEKSGAGKSKEFSEKSDYSCQQSRKFPENDDVIDASPCRKQVPAGPSNQKNLYLNGSEDISAQVLPVNYASPTGIAKPGGSSYAENVAPDKNKCSLEANDFIENDVFNALTPPNSPPSQTPGKSFQISRDHLKTEKQGSSGKRSSSGKSSDNPQNKAARAYVRNMPGSRRGAFLDLLGGSPENKRKKTDSSKSDKDSSSARKKSGHASVGSYPVAAGVSH*