Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0765530 | sp|D4Q9Z4|SGT2_SOYBN | 62.562 | 203 | 76 | 0 | 6 | 208 | 2 | 204 | 8.08e-89 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0765530 | G7KDU4 | 92.823 | 209 | 15 | 0 | 1 | 209 | 1 | 209 | 2.95e-139 | 408 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0022900 | Msa0765530 | 0.806287 | 8.850876e-50 | -8.615850e-47 |
| Msa0765530 | Msa0894040 | 0.802502 | 5.441463e-49 | -8.615850e-47 |
| Msa0765530 | Msa0927930 | 0.810669 | 1.027469e-50 | -8.615850e-47 |
| Msa0765530 | Msa1383560 | 0.838791 | 2.331261e-57 | -8.615850e-47 |
| Msa0765530 | Msa1445890 | 0.802553 | 5.311471e-49 | -8.615850e-47 |
| Msa0209060 | Msa0765530 | 0.802500 | 5.447807e-49 | -8.615850e-47 |
| Msa0395070 | Msa0765530 | 0.807604 | 4.661584e-50 | -8.615850e-47 |
| Msa0288540 | Msa0765530 | 0.802238 | 6.168592e-49 | -8.615850e-47 |
| Msa0326770 | Msa0765530 | 0.828497 | 8.701089e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0765530 | MtrunA17_Chr5g0416741 | 92.823 | 209 | 15 | 0 | 1 | 209 | 1 | 209 | 5.68e-143 | 408 |
| Msa0765530 | MtrunA17_Chr6g0463891 | 66.667 | 210 | 68 | 2 | 2 | 209 | 1 | 210 | 1.81e-99 | 297 |
| Msa0765530 | MtrunA17_Chr7g0231651 | 65.072 | 209 | 72 | 1 | 2 | 209 | 3 | 211 | 5.12e-98 | 283 |
| Msa0765530 | MtrunA17_Chr5g0416821 | 65.385 | 208 | 71 | 1 | 2 | 208 | 3 | 210 | 6.81e-95 | 285 |
| Msa0765530 | MtrunA17_Chr5g0401851 | 61.111 | 198 | 77 | 0 | 12 | 209 | 9 | 206 | 2.93e-86 | 263 |
| Msa0765530 | MtrunA17_Chr8g0355221 | 57.635 | 203 | 83 | 2 | 8 | 209 | 10 | 210 | 4.34e-77 | 239 |
| Msa0765530 | MtrunA17_Chr1g0165701 | 56.158 | 203 | 86 | 2 | 8 | 209 | 10 | 210 | 1.52e-73 | 230 |
| Msa0765530 | MtrunA17_Chr7g0243921 | 52.020 | 198 | 90 | 1 | 5 | 202 | 32 | 224 | 4.96e-68 | 208 |
| Msa0765530 | MtrunA17_Chr7g0243951 | 51.500 | 200 | 97 | 0 | 3 | 202 | 1 | 200 | 1.35e-66 | 212 |
| Msa0765530 | MtrunA17_Chr7g0243801 | 51.515 | 198 | 95 | 1 | 8 | 204 | 2 | 199 | 4.35e-65 | 208 |
| Msa0765530 | MtrunA17_Chr7g0243771 | 52.041 | 196 | 93 | 1 | 10 | 204 | 3 | 198 | 2.94e-64 | 206 |
| Msa0765530 | MtrunA17_Chr7g0243681 | 51.531 | 196 | 95 | 0 | 3 | 198 | 1 | 196 | 3.21e-64 | 206 |
| Msa0765530 | MtrunA17_Chr7g0243931 | 50.500 | 200 | 99 | 0 | 3 | 202 | 7 | 206 | 8.14e-63 | 202 |
| Msa0765530 | MtrunA17_Chr7g0243861 | 50.505 | 198 | 98 | 0 | 5 | 202 | 6 | 203 | 3.08e-62 | 201 |
| Msa0765530 | MtrunA17_Chr7g0243761 | 51.256 | 199 | 97 | 0 | 3 | 201 | 1 | 199 | 1.31e-61 | 199 |
| Msa0765530 | MtrunA17_Chr4g0060511 | 48.276 | 203 | 102 | 1 | 3 | 202 | 1 | 203 | 2.69e-59 | 193 |
| Msa0765530 | MtrunA17_Chr7g0243631 | 50.794 | 189 | 93 | 0 | 10 | 198 | 10 | 198 | 3.15e-59 | 193 |
| Msa0765530 | MtrunA17_Chr7g0243871 | 46.569 | 204 | 109 | 0 | 6 | 209 | 2 | 205 | 4.42e-59 | 192 |
| Msa0765530 | MtrunA17_Chr7g0243781 | 48.485 | 198 | 102 | 0 | 6 | 203 | 2 | 199 | 6.30e-57 | 187 |
| Msa0765530 | MtrunA17_Chr7g0235051 | 45.500 | 200 | 79 | 1 | 3 | 202 | 1 | 170 | 6.95e-51 | 171 |
| Msa0765530 | MtrunA17_Chr7g0243811 | 49.718 | 177 | 84 | 1 | 27 | 203 | 3 | 174 | 8.69e-48 | 161 |
| Msa0765530 | MtrunA17_Chr7g0243851 | 40.704 | 199 | 76 | 1 | 3 | 201 | 1 | 157 | 2.71e-43 | 150 |
| Msa0765530 | MtrunA17_Chr4g0044501 | 38.000 | 200 | 120 | 2 | 8 | 205 | 5 | 202 | 1.95e-41 | 146 |
| Msa0765530 | MtrunA17_Chr8g0366391 | 34.653 | 202 | 128 | 2 | 6 | 205 | 2 | 201 | 6.13e-40 | 142 |
| Msa0765530 | MtrunA17_Chr7g0275681 | 35.204 | 196 | 124 | 2 | 15 | 207 | 14 | 209 | 1.48e-37 | 135 |
| Msa0765530 | MtrunA17_Chr7g0265141 | 35.533 | 197 | 125 | 1 | 7 | 201 | 3 | 199 | 2.19e-36 | 132 |
| Msa0765530 | MtrunA17_Chr5g0419761 | 36.979 | 192 | 114 | 4 | 8 | 194 | 4 | 193 | 7.81e-36 | 131 |
| Msa0765530 | MtrunA17_Chr4g0066151 | 36.757 | 185 | 108 | 2 | 5 | 189 | 10 | 185 | 3.68e-35 | 129 |
| Msa0765530 | MtrunA17_Chr7g0265041 | 33.493 | 209 | 132 | 3 | 1 | 204 | 1 | 207 | 5.89e-34 | 126 |
| Msa0765530 | MtrunA17_Chr4g0066131 | 35.638 | 188 | 112 | 2 | 1 | 188 | 1 | 179 | 6.32e-34 | 125 |
| Msa0765530 | MtrunA17_Chr4g0066261 | 36.957 | 184 | 105 | 3 | 12 | 189 | 10 | 188 | 1.98e-33 | 124 |
| Msa0765530 | MtrunA17_Chr4g0044511 | 30.693 | 202 | 134 | 2 | 8 | 208 | 35 | 231 | 3.27e-33 | 124 |
| Msa0765530 | MtrunA17_Chr4g0014061 | 33.508 | 191 | 125 | 1 | 8 | 198 | 4 | 192 | 7.89e-33 | 123 |
| Msa0765530 | MtrunA17_Chr5g0419771 | 37.566 | 189 | 111 | 4 | 10 | 194 | 33 | 218 | 2.16e-32 | 122 |
| Msa0765530 | MtrunA17_Chr4g0066221 | 36.413 | 184 | 104 | 4 | 12 | 189 | 10 | 186 | 3.13e-32 | 121 |
| Msa0765530 | MtrunA17_Chr2g0294931 | 37.017 | 181 | 109 | 2 | 12 | 189 | 10 | 188 | 3.72e-32 | 121 |
| Msa0765530 | MtrunA17_Chr2g0294921 | 35.912 | 181 | 111 | 2 | 12 | 189 | 159 | 337 | 5.22e-32 | 122 |
| Msa0765530 | MtrunA17_Chr4g0044521 | 35.323 | 201 | 119 | 2 | 8 | 208 | 4 | 193 | 1.46e-31 | 119 |
| Msa0765530 | MtrunA17_Chr1g0179901 | 34.737 | 190 | 119 | 3 | 8 | 194 | 11 | 198 | 5.05e-31 | 118 |
| Msa0765530 | MtrunA17_Chr8g0374971 | 36.517 | 178 | 105 | 2 | 12 | 186 | 11 | 183 | 2.76e-30 | 116 |
| Msa0765530 | MtrunA17_Chr4g0066201 | 39.216 | 153 | 86 | 2 | 12 | 164 | 17 | 162 | 6.89e-30 | 115 |
| Msa0765530 | MtrunA17_Chr4g0066171 | 38.562 | 153 | 86 | 2 | 12 | 164 | 20 | 164 | 9.80e-30 | 114 |
| Msa0765530 | MtrunA17_Chr7g0265111 | 33.155 | 187 | 119 | 2 | 10 | 194 | 36 | 218 | 1.38e-29 | 114 |
| Msa0765530 | MtrunA17_Chr4g0044481 | 35.638 | 188 | 95 | 1 | 7 | 194 | 21 | 182 | 2.34e-29 | 112 |
| Msa0765530 | MtrunA17_Chr7g0265101 | 35.714 | 182 | 113 | 3 | 15 | 192 | 11 | 192 | 9.37e-29 | 112 |
| Msa0765530 | MtrunA17_Chr8g0374931 | 39.333 | 150 | 89 | 1 | 12 | 161 | 10 | 157 | 1.67e-28 | 111 |
| Msa0765530 | MtrunA17_Chr4g0011051 | 35.135 | 185 | 116 | 3 | 12 | 192 | 8 | 192 | 8.78e-28 | 109 |
| Msa0765530 | MtrunA17_Chr5g0410941 | 34.615 | 182 | 108 | 3 | 12 | 189 | 22 | 196 | 1.99e-27 | 108 |
| Msa0765530 | MtrunA17_Chr7g0265151 | 32.178 | 202 | 121 | 6 | 6 | 196 | 2 | 198 | 2.00e-26 | 105 |
| Msa0765530 | MtrunA17_Chr7g0243711 | 56.977 | 86 | 36 | 1 | 3 | 88 | 1 | 85 | 2.55e-26 | 103 |
| Msa0765530 | MtrunA17_Chr5g0428081 | 35.333 | 150 | 92 | 1 | 12 | 161 | 10 | 154 | 6.97e-26 | 103 |
| Msa0765530 | MtrunA17_Chr7g0265051 | 32.973 | 185 | 120 | 3 | 12 | 192 | 8 | 192 | 1.08e-25 | 103 |
| Msa0765530 | MtrunA17_Chr6g0463861 | 34.637 | 179 | 107 | 3 | 12 | 189 | 10 | 179 | 2.41e-25 | 102 |
| Msa0765530 | MtrunA17_Chr8g0374941 | 34.831 | 178 | 108 | 2 | 12 | 186 | 948 | 1120 | 2.87e-25 | 102 |
| Msa0765530 | MtrunA17_Chr8g0374941 | 34.831 | 178 | 108 | 2 | 12 | 186 | 10 | 182 | 3.65e-25 | 102 |
| Msa0765530 | MtrunA17_Chr8g0374941 | 34.270 | 178 | 109 | 2 | 12 | 186 | 475 | 647 | 1.00e-24 | 101 |
| Msa0765530 | MtrunA17_Chr7g0218311 | 38.028 | 142 | 86 | 1 | 12 | 153 | 15 | 154 | 5.72e-25 | 101 |
| Msa0765530 | MtrunA17_Chr4g0066281 | 33.152 | 184 | 111 | 2 | 8 | 191 | 7 | 178 | 6.12e-25 | 101 |
| Msa0765530 | MtrunA17_Chr8g0350881 | 33.708 | 178 | 116 | 1 | 12 | 189 | 11 | 186 | 2.52e-24 | 99.8 |
| Msa0765530 | MtrunA17_Chr4g0066141 | 36.242 | 149 | 86 | 2 | 41 | 189 | 8 | 147 | 7.38e-24 | 97.1 |
| Msa0765530 | MtrunA17_Chr8g0374901 | 32.044 | 181 | 113 | 3 | 12 | 189 | 11 | 184 | 4.05e-23 | 96.3 |
| Msa0765530 | MtrunA17_Chr6g0463851 | 32.796 | 186 | 110 | 3 | 12 | 189 | 10 | 188 | 5.87e-22 | 92.8 |
| Msa0765530 | MtrunA17_Chr7g0272701 | 35.537 | 121 | 73 | 3 | 90 | 205 | 11 | 131 | 4.91e-17 | 74.7 |
| Msa0765530 | MtrunA17_Chr1g0196851 | 30.380 | 158 | 95 | 4 | 4 | 158 | 37 | 182 | 5.09e-14 | 70.1 |
| Msa0765530 | MtrunA17_Chr2g0329111 | 27.638 | 199 | 127 | 7 | 14 | 203 | 7 | 197 | 4.44e-13 | 67.4 |
| Msa0765530 | MtrunA17_Chr5g0440421 | 27.143 | 210 | 135 | 7 | 7 | 202 | 2 | 207 | 2.56e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0765530 | AT4G34135.2 | 32.843 | 204 | 128 | 3 | 12 | 207 | 10 | 212 | 7.67e-36 | 129 |
| Msa0765530 | AT4G34135.1 | 33.333 | 192 | 120 | 2 | 12 | 195 | 10 | 201 | 1.16e-34 | 128 |
| Msa0765530 | AT4G34131.1 | 32.812 | 192 | 121 | 2 | 12 | 195 | 9 | 200 | 3.31e-33 | 124 |
| Msa0765530 | AT2G15480.1 | 29.717 | 212 | 140 | 3 | 5 | 208 | 2 | 212 | 1.01e-31 | 120 |
| Msa0765530 | AT2G15480.2 | 29.717 | 212 | 140 | 3 | 5 | 208 | 2 | 212 | 1.07e-31 | 120 |
| Msa0765530 | AT2G36750.1 | 31.959 | 194 | 123 | 3 | 12 | 196 | 9 | 202 | 3.39e-31 | 119 |
| Msa0765530 | AT3G53150.1 | 32.995 | 197 | 127 | 3 | 7 | 199 | 16 | 211 | 1.09e-30 | 117 |
| Msa0765530 | AT2G15490.2 | 30.601 | 183 | 119 | 2 | 14 | 188 | 8 | 190 | 1.24e-30 | 114 |
| Msa0765530 | AT2G15490.3 | 31.016 | 187 | 121 | 2 | 14 | 192 | 8 | 194 | 2.30e-29 | 114 |
| Msa0765530 | AT2G15490.1 | 31.016 | 187 | 121 | 2 | 14 | 192 | 8 | 194 | 2.61e-29 | 114 |
| Msa0765530 | AT4G34138.1 | 32.292 | 192 | 117 | 4 | 8 | 189 | 6 | 194 | 9.52e-29 | 112 |
| Msa0765530 | AT2G36780.1 | 29.703 | 202 | 136 | 3 | 11 | 209 | 12 | 210 | 5.03e-28 | 110 |
| Msa0765530 | AT2G36790.1 | 31.443 | 194 | 126 | 3 | 8 | 194 | 4 | 197 | 4.33e-27 | 107 |
| Msa0765530 | AT2G36770.1 | 30.108 | 186 | 127 | 2 | 12 | 194 | 13 | 198 | 9.00e-27 | 107 |
| Msa0765530 | AT2G36800.1 | 29.082 | 196 | 136 | 2 | 2 | 194 | 1 | 196 | 3.69e-26 | 105 |
| Msa0765530 | AT2G36760.1 | 29.730 | 185 | 127 | 2 | 12 | 193 | 13 | 197 | 4.85e-26 | 104 |
| Msa0765530 | AT3G53160.1 | 29.570 | 186 | 126 | 3 | 12 | 193 | 7 | 191 | 1.89e-22 | 94.7 |
| Msa0765530 | AT2G16890.4 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 1.05e-12 | 66.2 |
| Msa0765530 | AT2G16890.1 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 1.05e-12 | 66.2 |
| Msa0765530 | AT2G16890.3 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 2.09e-12 | 65.5 |
| Msa0765530 | AT2G16890.2 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 3.34e-12 | 65.1 |
| Msa0765530 | AT5G14860.1 | 28.495 | 186 | 111 | 6 | 15 | 183 | 10 | 190 | 2.09e-11 | 62.8 |
| Msa0765530 | AT1G10400.1 | 26.486 | 185 | 120 | 5 | 8 | 183 | 2 | 179 | 4.85e-11 | 61.6 |
Find 51 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGTCTTCGAAAATCTATTA+TGG | 0.276486 | 5_2:-26609333 | Msa0765530:CDS |
| GGTAGGTTTGCCACATAAAC+TGG | 0.325089 | 5_2:-26609085 | Msa0765530:CDS |
| CATAATTCCTATGGTTGATA+TGG | 0.330403 | 5_2:-26609550 | Msa0765530:CDS |
| GATTCTCTATTTGTGGTTGT+AGG | 0.355186 | 5_2:+26609301 | Msa0765520:intron |
| GTTGCCTGCGGTGGTGATTA+TGG | 0.365577 | 5_2:+26609494 | Msa0765520:intron |
| ATGAGAAAGTCAAATGCTTA+TGG | 0.375477 | 5_2:-26609033 | Msa0765530:CDS |
| AACAAGTTGGTCTTCCAGTT+GGG | 0.375978 | 5_2:-26609389 | Msa0765530:CDS |
| TTGAAGGTTTCCACCCCAAC+TGG | 0.385526 | 5_2:+26609375 | Msa0765520:intron |
| ACCTACCATCGTGAATTTAT+CGG | 0.396689 | 5_2:+26609101 | Msa0765520:intron |
| TTTGACTTTCTCATCCAATC+TGG | 0.409831 | 5_2:+26609042 | Msa0765520:intron |
| CAAGATTGCAATTACCAGAT+TGG | 0.417174 | 5_2:-26609056 | Msa0765530:CDS |
| TTAGTGACATGTTTCATCCT+TGG | 0.431972 | 5_2:-26609248 | Msa0765530:CDS |
| ACGATTGAAATCGCGGTCAA+TGG | 0.433731 | 5_2:+26609452 | Msa0765520:intron |
| GTAGAAGTTGCAGAAAAGTT+AGG | 0.443483 | 5_2:-26609222 | Msa0765530:CDS |
| TATGTGACTTGTAGATAAGA+AGG | 0.463270 | 5_2:+26609569 | Msa0765520:intron |
| GGAAGACCAACTTGTTTAGC+TGG | 0.463523 | 5_2:+26609396 | Msa0765520:intron |
| AGGAGGAGTATCGGCGTTGA+AGG | 0.463737 | 5_2:+26609359 | Msa0765520:intron |
| CTTGTCATCTCCAGTTTATG+TGG | 0.472070 | 5_2:+26609075 | Msa0765520:intron |
| TACAAGTCACATAATTCCTA+TGG | 0.510486 | 5_2:-26609559 | Msa0765530:CDS |
| GGTGACATCCACACCTTGCA+TGG | 0.510554 | 5_2:+26609515 | Msa0765520:intron |
| AAACAAGTTGGTCTTCCAGT+TGG | 0.513181 | 5_2:-26609390 | Msa0765530:CDS |
| AGCTGAAACGAATAACTTGA+AGG | 0.514317 | 5_2:-26609604 | Msa0765530:CDS |
| TCGTGAATTTATCGGAATCA+TGG | 0.515266 | 5_2:+26609109 | Msa0765520:intron |
| AACAACATGCAACTCACACC+AGG | 0.517115 | 5_2:-26609137 | Msa0765530:CDS |
| TGCTTATGGACAGTTAATGA+AGG | 0.517282 | 5_2:-26609019 | Msa0765530:CDS |
| TTCCTATGGTTGATATGGCA+AGG | 0.519211 | 5_2:-26609545 | Msa0765530:CDS |
| TCTGCAACTTCTACAGTCCA+AGG | 0.525503 | 5_2:+26609231 | Msa0765520:intron |
| TCGATCTGCGGTTCACACGT+TGG | 0.527754 | 5_2:-26609160 | Msa0765530:CDS |
| GAGTTTCCAGCTAAACAAGT+TGG | 0.555597 | 5_2:-26609402 | Msa0765530:CDS |
| ATGTGACTTGTAGATAAGAA+GGG | 0.556016 | 5_2:+26609570 | Msa0765520:intron |
| ACAAGTTGGTCTTCCAGTTG+GGG | 0.557963 | 5_2:-26609388 | Msa0765530:CDS |
| TCCTATGGTTGATATGGCAA+GGG | 0.565831 | 5_2:-26609544 | Msa0765530:CDS |
| TCCGATAAATTCACGATGGT+AGG | 0.572144 | 5_2:-26609102 | Msa0765530:CDS |
| AATTGCAGCGTTGCCTGCGG+TGG | 0.581566 | 5_2:+26609485 | Msa0765520:intron |
| AGTTGGTCTTCCAGTTGGGG+TGG | 0.582666 | 5_2:-26609385 | Msa0765530:CDS |
| ATTGACCGCGATTTCAATCG+TGG | 0.585780 | 5_2:-26609450 | Msa0765530:CDS |
| AGACGTGTTAGGAGGAGTAT+CGG | 0.603373 | 5_2:+26609350 | Msa0765520:intron |
| GACTTGTAGATAAGAAGGGA+AGG | 0.612405 | 5_2:+26609574 | Msa0765520:intron |
| GTCACCATAATCACCACCGC+AGG | 0.624053 | 5_2:-26609498 | Msa0765530:CDS |
| ATCGGAATCATGGTCCACCC+TGG | 0.625345 | 5_2:+26609119 | Msa0765520:intron |
| GCAAGGGTATTTGCCATGCA+AGG | 0.625587 | 5_2:-26609528 | Msa0765530:CDS |
| ACCCTTGCCATATCAACCAT+AGG | 0.629141 | 5_2:+26609543 | Msa0765520:intron |
| CAGAAAAGTTAGGCATTCCG+CGG | 0.633351 | 5_2:-26609212 | Msa0765530:CDS |
| GGTATTTGCCATGCAAGGTG+TGG | 0.648806 | 5_2:-26609523 | Msa0765530:CDS |
| AGCGACCACGATTGAAATCG+CGG | 0.652411 | 5_2:+26609445 | Msa0765520:intron |
| GAAAATTGCAGCGTTGCCTG+CGG | 0.659981 | 5_2:+26609482 | Msa0765520:intron |
| GAGTGTTCTCTCTCGATCTG+CGG | 0.674850 | 5_2:-26609172 | Msa0765530:CDS |
| ACAACATGCAACTCACACCA+GGG | 0.703158 | 5_2:-26609136 | Msa0765530:CDS |
| ACATGCAACTCACACCAGGG+TGG | 0.720674 | 5_2:-26609133 | Msa0765530:CDS |
| TGATTCCGATAAATTCACGA+TGG | 0.728112 | 5_2:-26609106 | Msa0765530:CDS |
| GCAGCATAGAAAATAATCCG+CGG | 0.733001 | 5_2:+26609195 | Msa0765520:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAACAGATTCTCTATTTG+TGG | + | chr5_2:26609344-26609363 | Msa0765520:intron | 25.0% |
| ! | ATGTGACTTGTAGATAAGAA+GGG | + | chr5_2:26609068-26609087 | Msa0765520:intron | 30.0% |
| ! | TATGTGACTTGTAGATAAGA+AGG | + | chr5_2:26609069-26609088 | Msa0765520:intron | 30.0% |
| TACAAGTCACATAATTCCTA+TGG | - | chr5_2:26609076-26609095 | Msa0765530:CDS | 30.0% | |
| ! | CATAATTCCTATGGTTGATA+TGG | - | chr5_2:26609085-26609104 | Msa0765530:CDS | 30.0% |
| ! | CTCCATAAAAACACATGTTT+TGG | - | chr5_2:26609211-26609230 | Msa0765530:CDS | 30.0% |
| !! | CTCCAAAACATGTGTTTTTA+TGG | + | chr5_2:26609216-26609235 | Msa0765520:intron | 30.0% |
| ACGTCTTCGAAAATCTATTA+TGG | - | chr5_2:26609302-26609321 | Msa0765530:CDS | 30.0% | |
| ATGAGAAAGTCAAATGCTTA+TGG | - | chr5_2:26609602-26609621 | Msa0765530:CDS | 30.0% | |
| AGCTGAAACGAATAACTTGA+AGG | - | chr5_2:26609031-26609050 | Msa0765530:CDS | 35.0% | |
| ! | TAGATTTTCGAAGACGTGTT+AGG | + | chr5_2:26609299-26609318 | Msa0765520:intron | 35.0% |
| GATTCTCTATTTGTGGTTGT+AGG | + | chr5_2:26609337-26609356 | Msa0765520:intron | 35.0% | |
| TTAGTGACATGTTTCATCCT+TGG | - | chr5_2:26609387-26609406 | Msa0765530:CDS | 35.0% | |
| GTAGAAGTTGCAGAAAAGTT+AGG | - | chr5_2:26609413-26609432 | Msa0765530:CDS | 35.0% | |
| TCGTGAATTTATCGGAATCA+TGG | + | chr5_2:26609529-26609548 | Msa0765520:intron | 35.0% | |
| TGATTCCGATAAATTCACGA+TGG | - | chr5_2:26609529-26609548 | Msa0765530:CDS | 35.0% | |
| ACCTACCATCGTGAATTTAT+CGG | + | chr5_2:26609537-26609556 | Msa0765520:intron | 35.0% | |
| CAAGATTGCAATTACCAGAT+TGG | - | chr5_2:26609579-26609598 | Msa0765530:CDS | 35.0% | |
| ! | TTTGACTTTCTCATCCAATC+TGG | + | chr5_2:26609596-26609615 | Msa0765520:intron | 35.0% |
| TGCTTATGGACAGTTAATGA+AGG | - | chr5_2:26609616-26609635 | Msa0765530:CDS | 35.0% | |
| GACTTGTAGATAAGAAGGGA+AGG | + | chr5_2:26609064-26609083 | Msa0765520:intron | 40.0% | |
| ! | TTCCTATGGTTGATATGGCA+AGG | - | chr5_2:26609090-26609109 | Msa0765530:CDS | 40.0% |
| ! | TCCTATGGTTGATATGGCAA+GGG | - | chr5_2:26609091-26609110 | Msa0765530:CDS | 40.0% |
| !! | AATCGCGGTCAATGGATTTT+TGG | + | chr5_2:26609178-26609197 | Msa0765520:intron | 40.0% |
| GAGTTTCCAGCTAAACAAGT+TGG | - | chr5_2:26609233-26609252 | Msa0765530:CDS | 40.0% | |
| !! | AAACAAGTTGGTCTTCCAGT+TGG | - | chr5_2:26609245-26609264 | Msa0765530:CDS | 40.0% |
| !! | AACAAGTTGGTCTTCCAGTT+GGG | - | chr5_2:26609246-26609265 | Msa0765530:CDS | 40.0% |
| ! | ATTTTCGAAGACGTGTTAGG+AGG | + | chr5_2:26609296-26609315 | Msa0765520:intron | 40.0% |
| GCAGCATAGAAAATAATCCG+CGG | + | chr5_2:26609443-26609462 | Msa0765520:intron | 40.0% | |
| TCCGATAAATTCACGATGGT+AGG | - | chr5_2:26609533-26609552 | Msa0765530:CDS | 40.0% | |
| CTTGTCATCTCCAGTTTATG+TGG | + | chr5_2:26609563-26609582 | Msa0765520:intron | 40.0% | |
| ACCCTTGCCATATCAACCAT+AGG | + | chr5_2:26609095-26609114 | Msa0765520:intron | 45.0% | |
| ACGATTGAAATCGCGGTCAA+TGG | + | chr5_2:26609186-26609205 | Msa0765520:intron | 45.0% | |
| ATTGACCGCGATTTCAATCG+TGG | - | chr5_2:26609185-26609204 | Msa0765530:CDS | 45.0% | |
| ! | GGAAGACCAACTTGTTTAGC+TGG | + | chr5_2:26609242-26609261 | Msa0765520:intron | 45.0% |
| !! | ACAAGTTGGTCTTCCAGTTG+GGG | - | chr5_2:26609247-26609266 | Msa0765530:CDS | 45.0% |
| ! | AGACGTGTTAGGAGGAGTAT+CGG | + | chr5_2:26609288-26609307 | Msa0765520:intron | 45.0% |
| TCTGCAACTTCTACAGTCCA+AGG | + | chr5_2:26609407-26609426 | Msa0765520:intron | 45.0% | |
| CAGAAAAGTTAGGCATTCCG+CGG | - | chr5_2:26609423-26609442 | Msa0765530:CDS | 45.0% | |
| AACAACATGCAACTCACACC+AGG | - | chr5_2:26609498-26609517 | Msa0765530:CDS | 45.0% | |
| ACAACATGCAACTCACACCA+GGG | - | chr5_2:26609499-26609518 | Msa0765530:CDS | 45.0% | |
| GGTAGGTTTGCCACATAAAC+TGG | - | chr5_2:26609550-26609569 | Msa0765530:CDS | 45.0% | |
| !! | GCAAGGGTATTTGCCATGCA+AGG | - | chr5_2:26609107-26609126 | Msa0765530:CDS | 50.0% |
| GGTATTTGCCATGCAAGGTG+TGG | - | chr5_2:26609112-26609131 | Msa0765530:CDS | 50.0% | |
| GAAAATTGCAGCGTTGCCTG+CGG | + | chr5_2:26609156-26609175 | Msa0765520:intron | 50.0% | |
| AGCGACCACGATTGAAATCG+CGG | + | chr5_2:26609193-26609212 | Msa0765520:intron | 50.0% | |
| TTGAAGGTTTCCACCCCAAC+TGG | + | chr5_2:26609263-26609282 | Msa0765520:intron | 50.0% | |
| GAGTGTTCTCTCTCGATCTG+CGG | - | chr5_2:26609463-26609482 | Msa0765530:CDS | 50.0% | |
| GGTGACATCCACACCTTGCA+TGG | + | chr5_2:26609123-26609142 | Msa0765520:intron | 55.0% | |
| GTCACCATAATCACCACCGC+AGG | - | chr5_2:26609137-26609156 | Msa0765530:CDS | 55.0% | |
| GTTGCCTGCGGTGGTGATTA+TGG | + | chr5_2:26609144-26609163 | Msa0765520:intron | 55.0% | |
| !! | AGTTGGTCTTCCAGTTGGGG+TGG | - | chr5_2:26609250-26609269 | Msa0765530:CDS | 55.0% |
| !! | AGGAGGAGTATCGGCGTTGA+AGG | + | chr5_2:26609279-26609298 | Msa0765520:intron | 55.0% |
| TCGATCTGCGGTTCACACGT+TGG | - | chr5_2:26609475-26609494 | Msa0765530:CDS | 55.0% | |
| ACATGCAACTCACACCAGGG+TGG | - | chr5_2:26609502-26609521 | Msa0765530:CDS | 55.0% | |
| ATCGGAATCATGGTCCACCC+TGG | + | chr5_2:26609519-26609538 | Msa0765520:intron | 55.0% | |
| AATTGCAGCGTTGCCTGCGG+TGG | + | chr5_2:26609153-26609172 | Msa0765520:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 26609014 | 26609643 | 26609014 | ID=Msa0765530;Name=Msa0765530 |
| chr5_2 | mRNA | 26609014 | 26609643 | 26609014 | ID=Msa0765530-mRNA-1;Parent=Msa0765530;Name=Msa0765530-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|209 |
| chr5_2 | exon | 26609014 | 26609643 | 26609014 | ID=Msa0765530-mRNA-1:exon:10540;Parent=Msa0765530-mRNA-1 |
| chr5_2 | CDS | 26609014 | 26609643 | 26609014 | ID=Msa0765530-mRNA-1:cds;Parent=Msa0765530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
