AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1445890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1445890 MtrunA17_Chr5g0432341 49.367 79 18 2 19 76 246 323 2.76e-15 68.2
Msa1445890 MtrunA17_Chr5g0432331 49.367 79 18 2 19 76 277 354 8.16e-15 67.0
Msa1445890 MtrunA17_Chr5g0432401 46.053 76 19 2 19 73 211 285 8.91e-15 66.6
Msa1445890 MtrunA17_Chr5g0432361 41.975 81 25 2 14 73 29 108 4.33e-14 61.6
Msa1445890 MtrunA17_Chr5g0432351 48.052 77 18 2 19 74 246 321 1.67e-13 63.2
Msa1445890 MtrunA17_Chr3g0107881 47.945 73 16 2 23 74 253 324 7.14e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGATTTATTGTACAAAA+AGG 0.207420 tig0031232:-35415 Msa1445890:CDS
TATTAGAACCATATATTATA+AGG 0.252483 tig0031232:-35433 Msa1445890:CDS
GCCTATTACTGGCAGAATAA+TGG 0.270633 tig0031232:-35255 Msa1445890:CDS
CAATAAATCCTTATAATATA+TGG 0.294284 tig0031232:+35425 Msa1445890:intergenic
CCCATTATTCTGCCAGTAAT+AGG 0.308962 tig0031232:+35254 Msa1445890:intergenic
CCTATTACTGGCAGAATAAT+GGG 0.377412 tig0031232:-35254 Msa1445890:CDS
AGTAACATGAAGCCTATTAC+TGG 0.410932 tig0031232:-35266 Msa1445890:CDS
GCAGAATAATGGGTGAGATT+AGG 0.482122 tig0031232:-35244 Msa1445890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTATTTTAATATTT+CGG - tig0031232:36598-36617 Msa1445890:intron 0.0%
!!! AAATTTATAATTTATTTTTT+CGG - tig0031232:36671-36690 Msa1445890:intron 0.0%
!!! AAAAATTTTTAAAATTTTTT+CGG - tig0031232:36708-36727 Msa1445890:intron 0.0%
!! AATAATACTTTCATATATAT+TGG + tig0031232:35611-35630 Msa1445890:intergenic 10.0%
!!! AATTTTTTTTTGTTTGAAAA+TGG - tig0031232:36038-36057 Msa1445890:intron 10.0%
!!! AAATTTATTTTCAGATTTTT+CGG + tig0031232:37207-37226 Msa1445890:intergenic 10.0%
!! AAATATATTTCATAAATTTC+CGG - tig0031232:36535-36554 Msa1445890:intron 10.0%
!! TCGAAAATTTAAAAATATTT+CGG + tig0031232:36854-36873 Msa1445890:intergenic 10.0%
!!! AAATTTATTTTCAGATTTTT+TGG - tig0031232:37207-37226 Msa1445890:intron 10.0%
!!! TATAATTTTCAGATTTTTTT+CGG - tig0031232:37279-37298 Msa1445890:intron 10.0%
!! CTGAAAATTATAAAAAATTT+CGG + tig0031232:36336-36355 Msa1445890:intergenic 10.0%
!!! TATAATTTTCAGAATTTTTT+CGG - tig0031232:37351-37370 Msa1445890:intron 10.0%
!!! AATTTTTTTTTGTTTGAAAA+TGG + tig0031232:36038-36057 Msa1445890:intergenic 10.0%
!! AATTTATCATAAAATATAAG+TGG + tig0031232:37678-37697 Msa1445890:intergenic 10.0%
!! TTTATGTTAGTATAAATTAT+AGG - tig0031232:38421-38440 Msa1445890:intron 10.0%
!! AGTTATGTAATATTATATGT+TGG - tig0031232:35439-35458 Msa1445890:CDS 15.0%
!!! CTTTGTATTATTTAAGTTAT+AGG - tig0031232:35467-35486 Msa1445890:intron 15.0%
!!! AATTTGGAACTTTAAATAAA+AGG - tig0031232:35678-35697 Msa1445890:intron 15.0%
!!! TCAAACTTTTTTTTTTTACA+GGG + tig0031232:36004-36023 Msa1445890:intergenic 15.0%
!!! TTCAAACTTTTTTTTTTTAC+AGG + tig0031232:36005-36024 Msa1445890:intergenic 15.0%
!! CAAACAAAAAAAAATTTACA+GGG + tig0031232:37574-37593 Msa1445890:intergenic 15.0%
!! TCAAACAAAAAAAAATTTAC+AGG + tig0031232:37573-37592 Msa1445890:intergenic 15.0%
!!! AAATTTATTTTGGGATTTTT+TGG + tig0031232:36288-36307 Msa1445890:intergenic 15.0%
!!! TTTAAAATAATCATGAGATA+AGG - tig0031232:36887-36906 Msa1445890:intron 15.0%
!!! ATGACAATTTTTACTTTAAA+AGG + tig0031232:37040-37059 Msa1445890:intergenic 15.0%
!! TCAAACAAAAAAAAATTTAC+AGG - tig0031232:37573-37592 Msa1445890:intron 15.0%
!! CAAACAAAAAAAAATTTACA+GGG - tig0031232:37574-37593 Msa1445890:intron 15.0%
!!! ATTCAAACTTTTTTATTTAC+AGG - tig0031232:37602-37621 Msa1445890:intron 15.0%
!!! TTCAAACTTTTTTATTTACA+GGG - tig0031232:37603-37622 Msa1445890:intron 15.0%
!! TAAATAATAATAGATTCACA+TGG + tig0031232:38067-38086 Msa1445890:intergenic 15.0%
!! ATGATTAAAAAATTAATCAG+TGG + tig0031232:38274-38293 Msa1445890:intergenic 15.0%
!! TATTAGAACCATATATTATA+AGG - tig0031232:38450-38469 Msa1445890:intron 15.0%
!! CAATAAATCCTTATAATATA+TGG + tig0031232:38461-38480 Msa1445890:intergenic 15.0%
!! CAAATTAATTCTAATTACTC+TGG + tig0031232:35871-35890 Msa1445890:intergenic 20.0%
!! GAAAGAATAATACATACATT+TGG - tig0031232:35901-35920 Msa1445890:intron 20.0%
!! AGTTAATTAAACAAGTAGAA+GGG + tig0031232:36987-37006 Msa1445890:intergenic 20.0%
!! TGTTTAATTAACTGAATATG+CGG - tig0031232:36994-37013 Msa1445890:intron 20.0%
!!! TTGAAAATTGAGAGTATTTT+TGG + tig0031232:37827-37846 Msa1445890:intergenic 20.0%
!!! TATGTACATTTTGTATTGTA+GGG + tig0031232:38397-38416 Msa1445890:intergenic 20.0%
!! TAAGGATTTATTGTACAAAA+AGG - tig0031232:38468-38487 Msa1445890:intron 20.0%
!! AAGGATTTATTGTACAAAAA+GGG - tig0031232:38469-38488 Msa1445890:intron 20.0%
!!! CTTTTTTCTTTTCGAATTCA+CGG + tig0031232:35356-35375 None:intergenic 25.0%
!!! AAAAGCTCTAAAACGTAATA+AGG - tig0031232:35371-35390 Msa1445890:CDS 25.0%
!!! AAAGCTCTAAAACGTAATAA+GGG - tig0031232:35372-35391 Msa1445890:CDS 25.0%
!! GATGTTGAGTTTTTATGTTA+AGG - tig0031232:35544-35563 Msa1445890:intron 25.0%
! TATGTTAAGGTTCAATTGTT+TGG - tig0031232:35557-35576 Msa1445890:intron 25.0%
! ATGTTAAGGTTCAATTGTTT+GGG - tig0031232:35558-35577 Msa1445890:intron 25.0%
!!! GAGAATTTGACTTGTAAAAA+GGG + tig0031232:35739-35758 Msa1445890:intergenic 25.0%
!!! TGAGAATTTGACTTGTAAAA+AGG + tig0031232:35740-35759 Msa1445890:intergenic 25.0%
!!! CTCTGGTCTTTATTATTTTT+TGG + tig0031232:35854-35873 Msa1445890:intergenic 25.0%
! TGTAAGAGATCAACTTATAT+TGG - tig0031232:35929-35948 Msa1445890:intron 25.0%
! CAACATTAAGAAAGACTAAT+GGG - tig0031232:36912-36931 Msa1445890:intron 25.0%
! AACATTAAGAAAGACTAATG+GGG - tig0031232:36913-36932 Msa1445890:intron 25.0%
! TTAATTAAACAAGTAGAAGG+GGG + tig0031232:36985-37004 Msa1445890:intergenic 25.0%
! GTTAATTAAACAAGTAGAAG+GGG + tig0031232:36986-37005 Msa1445890:intergenic 25.0%
! CAGTTAATTAAACAAGTAGA+AGG + tig0031232:36988-37007 Msa1445890:intergenic 25.0%
! GTTTAATTAACTGAATATGC+GGG - tig0031232:36995-37014 Msa1445890:intron 25.0%
! TTTAATTAACTGAATATGCG+GGG - tig0031232:36996-37015 Msa1445890:intron 25.0%
!!! AATTGAGAGTATTTTTGGAA+AGG + tig0031232:37822-37841 Msa1445890:intergenic 25.0%
!! ATTAGGGTTTTTGTTACAAT+AGG - tig0031232:38224-38243 Msa1445890:intron 25.0%
!! ACTAACGATAGACTTTATTT+CGG - tig0031232:38332-38351 Msa1445890:intron 25.0%
!! ATGTACATTTTGTATTGTAG+GGG + tig0031232:38396-38415 Msa1445890:intergenic 25.0%
!! CTATGTACATTTTGTATTGT+AGG + tig0031232:38398-38417 Msa1445890:intergenic 25.0%
!!! TGGTTTTGAAAGTGATAAAT+TGG - tig0031232:38528-38547 Msa1445890:intron 25.0%
!! TATGCTTTTACTAATGACTT+TGG - tig0031232:38576-38595 Msa1445890:intron 25.0%
!! AAGCTCTAAAACGTAATAAG+GGG - tig0031232:35373-35392 Msa1445890:CDS 30.0%
GCAACATTAAGAAAGACTAA+TGG - tig0031232:36911-36930 Msa1445890:intron 30.0%
CATATATAGATCAACTTTGC+TGG - tig0031232:37088-37107 Msa1445890:intron 30.0%
TTCCTCTGTGCTTAATTATA+AGG + tig0031232:37711-37730 Msa1445890:intergenic 30.0%
TGTTACAATAGGAGCTATAA+AGG - tig0031232:38235-38254 Msa1445890:intron 30.0%
!! CAAGAGCTTTTTAAAGATGA+TGG - tig0031232:38508-38527 Msa1445890:intron 30.0%
!! TTCAAATTTTGACACTCGAC+AGG + tig0031232:35508-35527 Msa1445890:intergenic 35.0%
GCGATGATCAATGCTTAATT+TGG - tig0031232:35662-35681 Msa1445890:intron 35.0%
GAATTTCGTCCCTGTAATAT+AGG - tig0031232:35961-35980 Msa1445890:intron 35.0%
CAAAACACGCCTATATTACA+GGG + tig0031232:37634-37653 Msa1445890:intergenic 35.0%
TCAAAACACGCCTATATTAC+AGG + tig0031232:37633-37652 Msa1445890:intergenic 35.0%
!! TTTTGTTTGAAAATGGTCCC+TGG - tig0031232:36045-36064 Msa1445890:intron 35.0%
TTTGTTTGAAAATGGTCCCT+GGG - tig0031232:36046-36065 Msa1445890:intron 35.0%
TTTGTTTGAAAATGGTCCCT+GGG + tig0031232:36046-36065 Msa1445890:intergenic 35.0%
!! TTTTGTTTGAAAATGGTCCC+TGG + tig0031232:36045-36064 Msa1445890:intergenic 35.0%
TCAAAACACGCCTATATTAC+AGG - tig0031232:37633-37652 Msa1445890:intron 35.0%
CAAAACACGCCTATATTACA+GGG - tig0031232:37634-37653 Msa1445890:intron 35.0%
GAATTTCGTCCCTGTAATAT+AGG + tig0031232:35961-35980 Msa1445890:intergenic 35.0%
GTCCTTATAATTAAGCACAG+AGG - tig0031232:37706-37725 Msa1445890:intron 35.0%
! ACTACCTCTTTCAGCATTTT+TGG - tig0031232:37785-37804 Msa1445890:intron 35.0%
! CTACCTCTTTCAGCATTTTT+GGG - tig0031232:37786-37805 Msa1445890:intron 35.0%
ACATAAACCAATTGCTACTG+CGG + tig0031232:38140-38159 Msa1445890:intergenic 35.0%
TGGTTTATGTCAAATCCCTT+TGG - tig0031232:38150-38169 Msa1445890:intron 35.0%
GGTTTATGTCAAATCCCTTT+GGG - tig0031232:38151-38170 Msa1445890:intron 35.0%
GTTTATGTCAAATCCCTTTG+GGG - tig0031232:38152-38171 Msa1445890:intron 35.0%
TTTATGTCAAATCCCTTTGG+GGG - tig0031232:38153-38172 Msa1445890:intron 35.0%
TAATCTTGAGAGTCTCCAAA+TGG + tig0031232:38208-38227 Msa1445890:intergenic 35.0%
ATTTGGAGACTCTCAAGATT+AGG - tig0031232:38207-38226 Msa1445890:intron 35.0%
TTTGGAGACTCTCAAGATTA+GGG - tig0031232:38208-38227 Msa1445890:intron 35.0%
AGTAACATGAAGCCTATTAC+TGG - tig0031232:38617-38636 Msa1445890:intron 35.0%
CCTATTACTGGCAGAATAAT+GGG - tig0031232:38629-38648 Msa1445890:intron 35.0%
! GCATTGATCATCGCATCAAA+AGG + tig0031232:35656-35675 Msa1445890:intergenic 40.0%
AAGAAATCCGCAGTAGCAAT+TGG - tig0031232:38130-38149 Msa1445890:intron 40.0%
GCCTATTACTGGCAGAATAA+TGG - tig0031232:38628-38647 Msa1445890:intron 40.0%
CCCATTATTCTGCCAGTAAT+AGG + tig0031232:38632-38651 Msa1445890:intergenic 40.0%
!! GCAGAATAATGGGTGAGATT+AGG - tig0031232:38639-38658 Msa1445890:intron 40.0%
AGATGTAGCAGAAGTCTGAC+AGG - tig0031232:36942-36961 Msa1445890:intron 45.0%
CTGCCCAAAAATGCTGAAAG+AGG + tig0031232:37792-37811 Msa1445890:intergenic 45.0%
TCTCCAAATGGAGACTTCCA+TGG + tig0031232:38196-38215 Msa1445890:intergenic 45.0%
!!! TTTTTTATTTTTTAATTATG+TGG + tig0031232:36159-36178 Msa1445890:intergenic 5.0%
!!! TATTATTTTTAGATTTTTTA+TGG + tig0031232:36257-36276 Msa1445890:intergenic 5.0%
!!! ATGATTTTTAAATTTATTTT+GGG + tig0031232:37198-37217 Msa1445890:intergenic 5.0%
!!! TATGATTTTTAAATTTATTT+TGG + tig0031232:37197-37216 Msa1445890:intergenic 5.0%
!!! TATTAAAATAAATTTTTTTC+CGG + tig0031232:36557-36576 Msa1445890:intergenic 5.0%
!!! AAAATTTTTAAAATTTTTTC+GGG - tig0031232:36709-36728 Msa1445890:intron 5.0%
!!! TTACATTTTTTTAATTTTTT+CGG - tig0031232:36759-36778 Msa1445890:intron 5.0%
!!! TTTTAAAATTTAATATTATG+TGG - tig0031232:37449-37468 Msa1445890:intron 5.0%
!!! TGTATATATATATTTTTTTA+AGG - tig0031232:37990-38009 Msa1445890:intron 5.0%
TGCCAACTCAGCTGAACAGT+GGG + tig0031232:36072-36091 Msa1445890:intergenic 50.0%
TTGCCAACTCAGCTGAACAG+TGG + tig0031232:36073-36092 Msa1445890:intergenic 50.0%
! ACTGTTCAGCTGAGTTGGCA+AGG - tig0031232:36072-36091 Msa1445890:intron 50.0%
! CTGTTCAGCTGAGTTGGCAA+GGG - tig0031232:36073-36092 Msa1445890:intron 50.0%
! ACTGTGCAACTTTTGCCACG+TGG - tig0031232:36118-36137 Msa1445890:intron 50.0%
! ACTGTGCAACTTTTGCCACG+TGG + tig0031232:36118-36137 Msa1445890:intergenic 50.0%
TGTAGTAGCAGCTTCAGCCA+TGG - tig0031232:38176-38195 Msa1445890:intron 50.0%
CAGCCATGGAAGTCTCCATT+TGG - tig0031232:38190-38209 Msa1445890:intron 50.0%
! CAGCTGAGTTGGCAAGGGTT+TGG - tig0031232:36078-36097 Msa1445890:intron 55.0%
!! ACTGTAGCGCTGATGTGGCA+TGG + tig0031232:37536-37555 Msa1445890:intergenic 55.0%
ATGCCACATCAGCGCTACAG+TGG - tig0031232:37535-37554 Msa1445890:intron 55.0%
TGCCACATCAGCGCTACAGT+GGG - tig0031232:37536-37555 Msa1445890:intron 55.0%
GCTGCTACTACACCCCCAAA+GGG + tig0031232:38168-38187 Msa1445890:intergenic 55.0%
AGCTGCTACTACACCCCCAA+AGG + tig0031232:38169-38188 Msa1445890:intergenic 55.0%
TCAGCTGAACAGTGGGCCCA+GGG + tig0031232:36065-36084 Msa1445890:intergenic 60.0%
GGCCCACTGTTCAGCTGAGT+TGG - tig0031232:36067-36086 Msa1445890:intron 60.0%
TTGGCAAGGGTTTGGCCACG+TGG - tig0031232:36086-36105 Msa1445890:intron 60.0%
CACAGTCAGCAACTGCCACG+TGG + tig0031232:37504-37523 Msa1445890:intergenic 60.0%
!! TTTGCCACGTGGCACTGACG+TGG + tig0031232:37479-37498 Msa1445890:intergenic 60.0%
CACAGTCAGCAACTGCCACG+TGG - tig0031232:37504-37523 Msa1445890:intron 60.0%
CTCAGCTGAACAGTGGGCCC+AGG + tig0031232:36066-36085 Msa1445890:intergenic 65.0%
!! TTTGCCACGTGGCGCTGACG+TGG - tig0031232:36129-36148 Msa1445890:intron 65.0%
GTGGCATGGCATTCGCCACG+TGG + tig0031232:37522-37541 Msa1445890:intergenic 65.0%
!! GGCCCACTGTAGCGCTGATG+TGG + tig0031232:37541-37560 Msa1445890:intergenic 65.0%
ATCAGCGCTACAGTGGGCCC+AGG - tig0031232:37542-37561 Msa1445890:intron 65.0%
TCAGCGCTACAGTGGGCCCA+GGG - tig0031232:37543-37562 Msa1445890:intron 65.0%
CACGCCACGTCAGTGCCACG+TGG - tig0031232:37472-37491 Msa1445890:intron 70.0%
CACGCCACGTCAGCGCCACG+TGG + tig0031232:36136-36155 Msa1445890:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0031232 gene 35214 38691 35214 ID=Msa1445890;Name=Msa1445890
tig0031232 mRNA 35214 38691 35214 ID=Msa1445890-mRNA-1;Parent=Msa1445890;Name=Msa1445890-mRNA-1;_AED=0.54;_eAED=0.56;_QI=0|0|0|0.75|0|0|4|0|76
tig0031232 exon 38686 38691 38686 ID=Msa1445890-mRNA-1:exon:15189;Parent=Msa1445890-mRNA-1
tig0031232 exon 38377 38416 38377 ID=Msa1445890-mRNA-1:exon:15188;Parent=Msa1445890-mRNA-1
tig0031232 exon 35356 35462 35356 ID=Msa1445890-mRNA-1:exon:15187;Parent=Msa1445890-mRNA-1
tig0031232 exon 35214 35291 35214 ID=Msa1445890-mRNA-1:exon:15186;Parent=Msa1445890-mRNA-1
tig0031232 CDS 38686 38691 38686 ID=Msa1445890-mRNA-1:cds;Parent=Msa1445890-mRNA-1
tig0031232 CDS 38377 38416 38377 ID=Msa1445890-mRNA-1:cds;Parent=Msa1445890-mRNA-1
tig0031232 CDS 35356 35462 35356 ID=Msa1445890-mRNA-1:cds;Parent=Msa1445890-mRNA-1
tig0031232 CDS 35214 35291 35214 ID=Msa1445890-mRNA-1:cds;Parent=Msa1445890-mRNA-1
Gene Sequence

>Msa1445890

ATGGACGTTCAAATGACATCGACCTGTCGAGTGTCAAAATTTGAAAGAATCTTTATTAGAACCATATATTATAAGGATTTATTGTACAAAAAGGGTCTCTTTGATTCTGATCAAGAGCTTTTTAAAGATGATGGTTTTGAAAGTGATAAATTGATGAGTAACATGAAGCCTATTACTGGCAGAATAATGGGTGAGATTAGGCACAATTGCAGAAAAGTAAACTATATATGA

Protein sequence

>Msa1445890

MDVQMTSTCRVSKFERIFIRTIYYKDLLYKKGLFDSDQELFKDDGFESDKLMSNMKPITGRIMGEIRHNCRKVNYI*