Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1383560 | XP_003613714.1 | 92.823 | 209 | 15 | 0 | 1 | 209 | 1 | 209 | 6.18e-139 | 408 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1383560 | sp|D4Q9Z4|SGT2_SOYBN | 62.562 | 203 | 76 | 0 | 6 | 208 | 2 | 204 | 8.08e-89 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1383560 | G7KDU4 | 92.823 | 209 | 15 | 0 | 1 | 209 | 1 | 209 | 2.95e-139 | 408 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0096590 | Msa1383560 | 0.805522 | 1.281608e-49 | -8.615850e-47 |
| Msa1365450 | Msa1383560 | 0.849613 | 2.890786e-60 | -8.615850e-47 |
| Msa1383560 | Msa1431160 | 0.811735 | 6.035026e-51 | -8.615850e-47 |
| Msa1383560 | Msa1445890 | 0.835059 | 2.091269e-56 | -8.615850e-47 |
| Msa0632980 | Msa1383560 | 0.809093 | 2.243288e-50 | -8.615850e-47 |
| Msa0765530 | Msa1383560 | 0.838791 | 2.331261e-57 | -8.615850e-47 |
| Msa0209060 | Msa1383560 | 0.802732 | 4.879931e-49 | -8.615850e-47 |
| Msa0372840 | Msa1383560 | 0.912925 | 1.112787e-83 | -8.615850e-47 |
| Msa0439000 | Msa1383560 | 0.804873 | 1.752573e-49 | -8.615850e-47 |
| Msa0439370 | Msa1383560 | 0.834330 | 3.190479e-56 | -8.615850e-47 |
| Msa0987640 | Msa1383560 | 0.813847 | 2.079984e-51 | -8.615850e-47 |
| Msa0288540 | Msa1383560 | 0.802090 | 6.617237e-49 | -8.615850e-47 |
| Msa0326770 | Msa1383560 | 0.851067 | 1.129971e-60 | -8.615850e-47 |
| Msa0483080 | Msa1383560 | 0.813425 | 2.575919e-51 | -8.615850e-47 |
| Msa0894070 | Msa1383560 | 0.823103 | 1.658918e-53 | -8.615850e-47 |
| Msa0913550 | Msa1383560 | 0.815722 | 7.991170e-52 | -8.615850e-47 |
| Msa0946010 | Msa1383560 | 0.802847 | 4.620513e-49 | -8.615850e-47 |
| Msa0951150 | Msa1383560 | 0.809872 | 1.526237e-50 | -8.615850e-47 |
| Msa0959700 | Msa1383560 | 0.801895 | 7.255018e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1383560 | MtrunA17_Chr5g0416741 | 92.823 | 209 | 15 | 0 | 1 | 209 | 1 | 209 | 5.68e-143 | 408 |
| Msa1383560 | MtrunA17_Chr6g0463891 | 66.667 | 210 | 68 | 2 | 2 | 209 | 1 | 210 | 1.81e-99 | 297 |
| Msa1383560 | MtrunA17_Chr7g0231651 | 65.072 | 209 | 72 | 1 | 2 | 209 | 3 | 211 | 5.12e-98 | 283 |
| Msa1383560 | MtrunA17_Chr5g0416821 | 65.385 | 208 | 71 | 1 | 2 | 208 | 3 | 210 | 6.81e-95 | 285 |
| Msa1383560 | MtrunA17_Chr5g0401851 | 61.111 | 198 | 77 | 0 | 12 | 209 | 9 | 206 | 2.93e-86 | 263 |
| Msa1383560 | MtrunA17_Chr8g0355221 | 57.635 | 203 | 83 | 2 | 8 | 209 | 10 | 210 | 4.34e-77 | 239 |
| Msa1383560 | MtrunA17_Chr1g0165701 | 56.158 | 203 | 86 | 2 | 8 | 209 | 10 | 210 | 1.52e-73 | 230 |
| Msa1383560 | MtrunA17_Chr7g0243921 | 52.020 | 198 | 90 | 1 | 5 | 202 | 32 | 224 | 4.96e-68 | 208 |
| Msa1383560 | MtrunA17_Chr7g0243951 | 51.500 | 200 | 97 | 0 | 3 | 202 | 1 | 200 | 1.35e-66 | 212 |
| Msa1383560 | MtrunA17_Chr7g0243801 | 51.515 | 198 | 95 | 1 | 8 | 204 | 2 | 199 | 4.35e-65 | 208 |
| Msa1383560 | MtrunA17_Chr7g0243771 | 52.041 | 196 | 93 | 1 | 10 | 204 | 3 | 198 | 2.94e-64 | 206 |
| Msa1383560 | MtrunA17_Chr7g0243681 | 51.531 | 196 | 95 | 0 | 3 | 198 | 1 | 196 | 3.21e-64 | 206 |
| Msa1383560 | MtrunA17_Chr7g0243931 | 50.500 | 200 | 99 | 0 | 3 | 202 | 7 | 206 | 8.14e-63 | 202 |
| Msa1383560 | MtrunA17_Chr7g0243861 | 50.505 | 198 | 98 | 0 | 5 | 202 | 6 | 203 | 3.08e-62 | 201 |
| Msa1383560 | MtrunA17_Chr7g0243761 | 51.256 | 199 | 97 | 0 | 3 | 201 | 1 | 199 | 1.31e-61 | 199 |
| Msa1383560 | MtrunA17_Chr4g0060511 | 48.276 | 203 | 102 | 1 | 3 | 202 | 1 | 203 | 2.69e-59 | 193 |
| Msa1383560 | MtrunA17_Chr7g0243631 | 50.794 | 189 | 93 | 0 | 10 | 198 | 10 | 198 | 3.15e-59 | 193 |
| Msa1383560 | MtrunA17_Chr7g0243871 | 46.569 | 204 | 109 | 0 | 6 | 209 | 2 | 205 | 4.42e-59 | 192 |
| Msa1383560 | MtrunA17_Chr7g0243781 | 48.485 | 198 | 102 | 0 | 6 | 203 | 2 | 199 | 6.30e-57 | 187 |
| Msa1383560 | MtrunA17_Chr7g0235051 | 45.500 | 200 | 79 | 1 | 3 | 202 | 1 | 170 | 6.95e-51 | 171 |
| Msa1383560 | MtrunA17_Chr7g0243811 | 49.718 | 177 | 84 | 1 | 27 | 203 | 3 | 174 | 8.69e-48 | 161 |
| Msa1383560 | MtrunA17_Chr7g0243851 | 40.704 | 199 | 76 | 1 | 3 | 201 | 1 | 157 | 2.71e-43 | 150 |
| Msa1383560 | MtrunA17_Chr4g0044501 | 38.000 | 200 | 120 | 2 | 8 | 205 | 5 | 202 | 1.95e-41 | 146 |
| Msa1383560 | MtrunA17_Chr8g0366391 | 34.653 | 202 | 128 | 2 | 6 | 205 | 2 | 201 | 6.13e-40 | 142 |
| Msa1383560 | MtrunA17_Chr7g0275681 | 35.204 | 196 | 124 | 2 | 15 | 207 | 14 | 209 | 1.48e-37 | 135 |
| Msa1383560 | MtrunA17_Chr7g0265141 | 35.533 | 197 | 125 | 1 | 7 | 201 | 3 | 199 | 2.19e-36 | 132 |
| Msa1383560 | MtrunA17_Chr5g0419761 | 36.979 | 192 | 114 | 4 | 8 | 194 | 4 | 193 | 7.81e-36 | 131 |
| Msa1383560 | MtrunA17_Chr4g0066151 | 36.757 | 185 | 108 | 2 | 5 | 189 | 10 | 185 | 3.68e-35 | 129 |
| Msa1383560 | MtrunA17_Chr7g0265041 | 33.493 | 209 | 132 | 3 | 1 | 204 | 1 | 207 | 5.89e-34 | 126 |
| Msa1383560 | MtrunA17_Chr4g0066131 | 35.638 | 188 | 112 | 2 | 1 | 188 | 1 | 179 | 6.32e-34 | 125 |
| Msa1383560 | MtrunA17_Chr4g0066261 | 36.957 | 184 | 105 | 3 | 12 | 189 | 10 | 188 | 1.98e-33 | 124 |
| Msa1383560 | MtrunA17_Chr4g0044511 | 30.693 | 202 | 134 | 2 | 8 | 208 | 35 | 231 | 3.27e-33 | 124 |
| Msa1383560 | MtrunA17_Chr4g0014061 | 33.508 | 191 | 125 | 1 | 8 | 198 | 4 | 192 | 7.89e-33 | 123 |
| Msa1383560 | MtrunA17_Chr5g0419771 | 37.566 | 189 | 111 | 4 | 10 | 194 | 33 | 218 | 2.16e-32 | 122 |
| Msa1383560 | MtrunA17_Chr4g0066221 | 36.413 | 184 | 104 | 4 | 12 | 189 | 10 | 186 | 3.13e-32 | 121 |
| Msa1383560 | MtrunA17_Chr2g0294931 | 37.017 | 181 | 109 | 2 | 12 | 189 | 10 | 188 | 3.72e-32 | 121 |
| Msa1383560 | MtrunA17_Chr2g0294921 | 35.912 | 181 | 111 | 2 | 12 | 189 | 159 | 337 | 5.22e-32 | 122 |
| Msa1383560 | MtrunA17_Chr4g0044521 | 35.323 | 201 | 119 | 2 | 8 | 208 | 4 | 193 | 1.46e-31 | 119 |
| Msa1383560 | MtrunA17_Chr1g0179901 | 34.737 | 190 | 119 | 3 | 8 | 194 | 11 | 198 | 5.05e-31 | 118 |
| Msa1383560 | MtrunA17_Chr8g0374971 | 36.517 | 178 | 105 | 2 | 12 | 186 | 11 | 183 | 2.76e-30 | 116 |
| Msa1383560 | MtrunA17_Chr4g0066201 | 39.216 | 153 | 86 | 2 | 12 | 164 | 17 | 162 | 6.89e-30 | 115 |
| Msa1383560 | MtrunA17_Chr4g0066171 | 38.562 | 153 | 86 | 2 | 12 | 164 | 20 | 164 | 9.80e-30 | 114 |
| Msa1383560 | MtrunA17_Chr7g0265111 | 33.155 | 187 | 119 | 2 | 10 | 194 | 36 | 218 | 1.38e-29 | 114 |
| Msa1383560 | MtrunA17_Chr4g0044481 | 35.638 | 188 | 95 | 1 | 7 | 194 | 21 | 182 | 2.34e-29 | 112 |
| Msa1383560 | MtrunA17_Chr7g0265101 | 35.714 | 182 | 113 | 3 | 15 | 192 | 11 | 192 | 9.37e-29 | 112 |
| Msa1383560 | MtrunA17_Chr8g0374931 | 39.333 | 150 | 89 | 1 | 12 | 161 | 10 | 157 | 1.67e-28 | 111 |
| Msa1383560 | MtrunA17_Chr4g0011051 | 35.135 | 185 | 116 | 3 | 12 | 192 | 8 | 192 | 8.78e-28 | 109 |
| Msa1383560 | MtrunA17_Chr5g0410941 | 34.615 | 182 | 108 | 3 | 12 | 189 | 22 | 196 | 1.99e-27 | 108 |
| Msa1383560 | MtrunA17_Chr7g0265151 | 32.178 | 202 | 121 | 6 | 6 | 196 | 2 | 198 | 2.00e-26 | 105 |
| Msa1383560 | MtrunA17_Chr7g0243711 | 56.977 | 86 | 36 | 1 | 3 | 88 | 1 | 85 | 2.55e-26 | 103 |
| Msa1383560 | MtrunA17_Chr5g0428081 | 35.333 | 150 | 92 | 1 | 12 | 161 | 10 | 154 | 6.97e-26 | 103 |
| Msa1383560 | MtrunA17_Chr7g0265051 | 32.973 | 185 | 120 | 3 | 12 | 192 | 8 | 192 | 1.08e-25 | 103 |
| Msa1383560 | MtrunA17_Chr6g0463861 | 34.637 | 179 | 107 | 3 | 12 | 189 | 10 | 179 | 2.41e-25 | 102 |
| Msa1383560 | MtrunA17_Chr8g0374941 | 34.831 | 178 | 108 | 2 | 12 | 186 | 948 | 1120 | 2.87e-25 | 102 |
| Msa1383560 | MtrunA17_Chr8g0374941 | 34.831 | 178 | 108 | 2 | 12 | 186 | 10 | 182 | 3.65e-25 | 102 |
| Msa1383560 | MtrunA17_Chr8g0374941 | 34.270 | 178 | 109 | 2 | 12 | 186 | 475 | 647 | 1.00e-24 | 101 |
| Msa1383560 | MtrunA17_Chr7g0218311 | 38.028 | 142 | 86 | 1 | 12 | 153 | 15 | 154 | 5.72e-25 | 101 |
| Msa1383560 | MtrunA17_Chr4g0066281 | 33.152 | 184 | 111 | 2 | 8 | 191 | 7 | 178 | 6.12e-25 | 101 |
| Msa1383560 | MtrunA17_Chr8g0350881 | 33.708 | 178 | 116 | 1 | 12 | 189 | 11 | 186 | 2.52e-24 | 99.8 |
| Msa1383560 | MtrunA17_Chr4g0066141 | 36.242 | 149 | 86 | 2 | 41 | 189 | 8 | 147 | 7.38e-24 | 97.1 |
| Msa1383560 | MtrunA17_Chr8g0374901 | 32.044 | 181 | 113 | 3 | 12 | 189 | 11 | 184 | 4.05e-23 | 96.3 |
| Msa1383560 | MtrunA17_Chr6g0463851 | 32.796 | 186 | 110 | 3 | 12 | 189 | 10 | 188 | 5.87e-22 | 92.8 |
| Msa1383560 | MtrunA17_Chr7g0272701 | 35.537 | 121 | 73 | 3 | 90 | 205 | 11 | 131 | 4.91e-17 | 74.7 |
| Msa1383560 | MtrunA17_Chr1g0196851 | 30.380 | 158 | 95 | 4 | 4 | 158 | 37 | 182 | 5.09e-14 | 70.1 |
| Msa1383560 | MtrunA17_Chr2g0329111 | 27.638 | 199 | 127 | 7 | 14 | 203 | 7 | 197 | 4.44e-13 | 67.4 |
| Msa1383560 | MtrunA17_Chr5g0440421 | 27.143 | 210 | 135 | 7 | 7 | 202 | 2 | 207 | 2.56e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1383560 | AT4G34135.2 | 32.843 | 204 | 128 | 3 | 12 | 207 | 10 | 212 | 7.67e-36 | 129 |
| Msa1383560 | AT4G34135.1 | 33.333 | 192 | 120 | 2 | 12 | 195 | 10 | 201 | 1.16e-34 | 128 |
| Msa1383560 | AT4G34131.1 | 32.812 | 192 | 121 | 2 | 12 | 195 | 9 | 200 | 3.31e-33 | 124 |
| Msa1383560 | AT2G15480.1 | 29.717 | 212 | 140 | 3 | 5 | 208 | 2 | 212 | 1.01e-31 | 120 |
| Msa1383560 | AT2G15480.2 | 29.717 | 212 | 140 | 3 | 5 | 208 | 2 | 212 | 1.07e-31 | 120 |
| Msa1383560 | AT2G36750.1 | 31.959 | 194 | 123 | 3 | 12 | 196 | 9 | 202 | 3.39e-31 | 119 |
| Msa1383560 | AT3G53150.1 | 32.995 | 197 | 127 | 3 | 7 | 199 | 16 | 211 | 1.09e-30 | 117 |
| Msa1383560 | AT2G15490.2 | 30.601 | 183 | 119 | 2 | 14 | 188 | 8 | 190 | 1.24e-30 | 114 |
| Msa1383560 | AT2G15490.3 | 31.016 | 187 | 121 | 2 | 14 | 192 | 8 | 194 | 2.30e-29 | 114 |
| Msa1383560 | AT2G15490.1 | 31.016 | 187 | 121 | 2 | 14 | 192 | 8 | 194 | 2.61e-29 | 114 |
| Msa1383560 | AT4G34138.1 | 32.292 | 192 | 117 | 4 | 8 | 189 | 6 | 194 | 9.52e-29 | 112 |
| Msa1383560 | AT2G36780.1 | 29.703 | 202 | 136 | 3 | 11 | 209 | 12 | 210 | 5.03e-28 | 110 |
| Msa1383560 | AT2G36790.1 | 31.443 | 194 | 126 | 3 | 8 | 194 | 4 | 197 | 4.33e-27 | 107 |
| Msa1383560 | AT2G36770.1 | 30.108 | 186 | 127 | 2 | 12 | 194 | 13 | 198 | 9.00e-27 | 107 |
| Msa1383560 | AT2G36800.1 | 29.082 | 196 | 136 | 2 | 2 | 194 | 1 | 196 | 3.69e-26 | 105 |
| Msa1383560 | AT2G36760.1 | 29.730 | 185 | 127 | 2 | 12 | 193 | 13 | 197 | 4.85e-26 | 104 |
| Msa1383560 | AT3G53160.1 | 29.570 | 186 | 126 | 3 | 12 | 193 | 7 | 191 | 1.89e-22 | 94.7 |
| Msa1383560 | AT2G16890.4 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 1.05e-12 | 66.2 |
| Msa1383560 | AT2G16890.1 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 1.05e-12 | 66.2 |
| Msa1383560 | AT2G16890.3 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 2.09e-12 | 65.5 |
| Msa1383560 | AT2G16890.2 | 30.857 | 175 | 107 | 5 | 9 | 175 | 5 | 173 | 3.34e-12 | 65.1 |
| Msa1383560 | AT5G14860.1 | 28.495 | 186 | 111 | 6 | 15 | 183 | 10 | 190 | 2.09e-11 | 62.8 |
| Msa1383560 | AT1G10400.1 | 26.486 | 185 | 120 | 5 | 8 | 183 | 2 | 179 | 4.85e-11 | 61.6 |
Find 51 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGTCTTCGAAAATCTATTA+TGG | 0.276486 | tig0020603:-86331 | Msa1383560:CDS |
| AATCGCGGTCAATGGACTTT+TGG | 0.288470 | tig0020603:+86458 | None:intergenic |
| AACAAGTTGGTCTTCCTGTT+GGG | 0.302531 | tig0020603:-86387 | Msa1383560:CDS |
| GGTAGGTTTGCCACATAAAC+TGG | 0.325089 | tig0020603:-86083 | Msa1383560:CDS |
| CATAATTCCTATGGTTGATA+TGG | 0.330403 | tig0020603:-86548 | Msa1383560:CDS |
| GATTCTCTATTTGTGGTTGT+AGG | 0.355186 | tig0020603:+86299 | None:intergenic |
| GTTGCCTGCGGTGGTGATTA+TGG | 0.365577 | tig0020603:+86492 | None:intergenic |
| ATGAGAAAGTCAAATGCTTA+TGG | 0.375477 | tig0020603:-86031 | Msa1383560:CDS |
| ACCTACCATCGTGAATTTAT+CGG | 0.396689 | tig0020603:+86099 | None:intergenic |
| TTTGACTTTCTCATCCAATC+TGG | 0.409831 | tig0020603:+86040 | None:intergenic |
| TTGAAGGTTTCCACCCCAAC+AGG | 0.415030 | tig0020603:+86373 | None:intergenic |
| CAAGATTGCAATTACCAGAT+TGG | 0.417174 | tig0020603:-86054 | Msa1383560:CDS |
| TTAGTGACATGTTTCATCCT+TGG | 0.431972 | tig0020603:-86246 | Msa1383560:CDS |
| ACGATTGAAATCGCGGTCAA+TGG | 0.442161 | tig0020603:+86450 | None:intergenic |
| GTAGAAGTTGCAGAAAAGTT+AGG | 0.443483 | tig0020603:-86220 | Msa1383560:CDS |
| GGAAGACCAACTTGTTTAGC+TGG | 0.459648 | tig0020603:+86394 | None:intergenic |
| AGGAGGAGTATCGGCGTTGA+AGG | 0.463737 | tig0020603:+86357 | None:intergenic |
| CTTGTCATCTCCAGTTTATG+TGG | 0.472070 | tig0020603:+86073 | None:intergenic |
| ACAAGTTGGTCTTCCTGTTG+GGG | 0.498942 | tig0020603:-86386 | Msa1383560:CDS |
| AAACAAGTTGGTCTTCCTGT+TGG | 0.499951 | tig0020603:-86388 | Msa1383560:CDS |
| TACAAGTCACATAATTCCTA+TGG | 0.510486 | tig0020603:-86557 | Msa1383560:CDS |
| GGTGACATCCACACCTTGCA+TGG | 0.510554 | tig0020603:+86513 | None:intergenic |
| AGCTGAAACGAATAACTTGA+AGG | 0.514317 | tig0020603:-86602 | Msa1383560:CDS |
| TCGTGAATTTATCGGAATCA+TGG | 0.515266 | tig0020603:+86107 | None:intergenic |
| TGCTTATGGACAGTTAATGA+AGG | 0.517282 | tig0020603:-86017 | Msa1383560:CDS |
| TTCCTATGGTTGATATGGCA+AGG | 0.519211 | tig0020603:-86543 | Msa1383560:CDS |
| AGCAACATGCAACTCACACC+AGG | 0.520766 | tig0020603:-86135 | Msa1383560:CDS |
| TCTGCAACTTCTACAGTCCA+AGG | 0.525503 | tig0020603:+86229 | None:intergenic |
| CAGAAAAGTTAGGCATTCCA+CGG | 0.549406 | tig0020603:-86210 | Msa1383560:CDS |
| GAGTTTCCAGCTAAACAAGT+TGG | 0.555597 | tig0020603:-86400 | Msa1383560:CDS |
| TCCTATGGTTGATATGGCAA+GGG | 0.565831 | tig0020603:-86542 | Msa1383560:CDS |
| TCCGATAAATTCACGATGGT+AGG | 0.572144 | tig0020603:-86100 | Msa1383560:CDS |
| AGTTGGTCTTCCTGTTGGGG+TGG | 0.573736 | tig0020603:-86383 | Msa1383560:CDS |
| TCGATCTGCGGTTCACACGT+TGG | 0.574981 | tig0020603:-86158 | Msa1383560:CDS |
| ATTGACCGCGATTTCAATCG+TGG | 0.578436 | tig0020603:-86448 | Msa1383560:CDS |
| AATTGCAGCGTTGCCTGCGG+TGG | 0.581566 | tig0020603:+86483 | None:intergenic |
| AGACGTGTTAGGAGGAGTAT+CGG | 0.603373 | tig0020603:+86348 | None:intergenic |
| ATGTGACTTGTAGATAAGAA+CGG | 0.609824 | tig0020603:+86568 | None:intergenic |
| GTCACCATAATCACCACCGC+AGG | 0.624053 | tig0020603:-86496 | Msa1383560:CDS |
| ATCGGAATCATGGTCCACCC+TGG | 0.625345 | tig0020603:+86117 | None:intergenic |
| GCAAGGGTATTTGCCATGCA+AGG | 0.625587 | tig0020603:-86526 | Msa1383560:CDS |
| ACCCTTGCCATATCAACCAT+AGG | 0.629141 | tig0020603:+86541 | None:intergenic |
| GACTTGTAGATAAGAACGGA+AGG | 0.638563 | tig0020603:+86572 | None:intergenic |
| GGTATTTGCCATGCAAGGTG+TGG | 0.648806 | tig0020603:-86521 | Msa1383560:CDS |
| AGCGACCACGATTGAAATCG+CGG | 0.652411 | tig0020603:+86443 | None:intergenic |
| GAAAATTGCAGCGTTGCCTG+CGG | 0.659981 | tig0020603:+86480 | None:intergenic |
| GAGTGTTCTCTCTCGATCTG+CGG | 0.674850 | tig0020603:-86170 | Msa1383560:CDS |
| GCAGCATAGAAAATAATCCG+TGG | 0.678787 | tig0020603:+86193 | None:intergenic |
| GCAACATGCAACTCACACCA+GGG | 0.706809 | tig0020603:-86134 | Msa1383560:CDS |
| ACATGCAACTCACACCAGGG+TGG | 0.724325 | tig0020603:-86131 | Msa1383560:CDS |
| TGATTCCGATAAATTCACGA+TGG | 0.728112 | tig0020603:-86104 | Msa1383560:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAACAGATTCTCTATTTG+TGG | + | tig0020603:86342-86361 | None:intergenic | 25.0% |
| ! | ATGTGACTTGTAGATAAGAA+CGG | + | tig0020603:86066-86085 | None:intergenic | 30.0% |
| TACAAGTCACATAATTCCTA+TGG | - | tig0020603:86074-86093 | Msa1383560:CDS | 30.0% | |
| ! | CATAATTCCTATGGTTGATA+TGG | - | tig0020603:86083-86102 | Msa1383560:CDS | 30.0% |
| ! | CTCCATAAAAACACATGTTT+TGG | - | tig0020603:86209-86228 | Msa1383560:CDS | 30.0% |
| !! | CTCCAAAACATGTGTTTTTA+TGG | + | tig0020603:86214-86233 | None:intergenic | 30.0% |
| ACGTCTTCGAAAATCTATTA+TGG | - | tig0020603:86300-86319 | Msa1383560:CDS | 30.0% | |
| ATGAGAAAGTCAAATGCTTA+TGG | - | tig0020603:86600-86619 | Msa1383560:CDS | 30.0% | |
| AGCTGAAACGAATAACTTGA+AGG | - | tig0020603:86029-86048 | Msa1383560:CDS | 35.0% | |
| ! | TAGATTTTCGAAGACGTGTT+AGG | + | tig0020603:86297-86316 | None:intergenic | 35.0% |
| GATTCTCTATTTGTGGTTGT+AGG | + | tig0020603:86335-86354 | None:intergenic | 35.0% | |
| TTAGTGACATGTTTCATCCT+TGG | - | tig0020603:86385-86404 | Msa1383560:CDS | 35.0% | |
| GTAGAAGTTGCAGAAAAGTT+AGG | - | tig0020603:86411-86430 | Msa1383560:CDS | 35.0% | |
| TCGTGAATTTATCGGAATCA+TGG | + | tig0020603:86527-86546 | None:intergenic | 35.0% | |
| TGATTCCGATAAATTCACGA+TGG | - | tig0020603:86527-86546 | Msa1383560:CDS | 35.0% | |
| ACCTACCATCGTGAATTTAT+CGG | + | tig0020603:86535-86554 | None:intergenic | 35.0% | |
| CAAGATTGCAATTACCAGAT+TGG | - | tig0020603:86577-86596 | Msa1383560:CDS | 35.0% | |
| ! | TTTGACTTTCTCATCCAATC+TGG | + | tig0020603:86594-86613 | None:intergenic | 35.0% |
| TGCTTATGGACAGTTAATGA+AGG | - | tig0020603:86614-86633 | Msa1383560:CDS | 35.0% | |
| GACTTGTAGATAAGAACGGA+AGG | + | tig0020603:86062-86081 | None:intergenic | 40.0% | |
| ! | TTCCTATGGTTGATATGGCA+AGG | - | tig0020603:86088-86107 | Msa1383560:CDS | 40.0% |
| ! | TCCTATGGTTGATATGGCAA+GGG | - | tig0020603:86089-86108 | Msa1383560:CDS | 40.0% |
| GAGTTTCCAGCTAAACAAGT+TGG | - | tig0020603:86231-86250 | Msa1383560:CDS | 40.0% | |
| !! | AAACAAGTTGGTCTTCCTGT+TGG | - | tig0020603:86243-86262 | Msa1383560:CDS | 40.0% |
| !! | AACAAGTTGGTCTTCCTGTT+GGG | - | tig0020603:86244-86263 | Msa1383560:CDS | 40.0% |
| ! | ATTTTCGAAGACGTGTTAGG+AGG | + | tig0020603:86294-86313 | None:intergenic | 40.0% |
| CAGAAAAGTTAGGCATTCCA+CGG | - | tig0020603:86421-86440 | Msa1383560:CDS | 40.0% | |
| GCAGCATAGAAAATAATCCG+TGG | + | tig0020603:86441-86460 | None:intergenic | 40.0% | |
| TCCGATAAATTCACGATGGT+AGG | - | tig0020603:86531-86550 | Msa1383560:CDS | 40.0% | |
| CTTGTCATCTCCAGTTTATG+TGG | + | tig0020603:86561-86580 | None:intergenic | 40.0% | |
| ACCCTTGCCATATCAACCAT+AGG | + | tig0020603:86093-86112 | None:intergenic | 45.0% | |
| !! | AATCGCGGTCAATGGACTTT+TGG | + | tig0020603:86176-86195 | None:intergenic | 45.0% |
| ACGATTGAAATCGCGGTCAA+TGG | + | tig0020603:86184-86203 | None:intergenic | 45.0% | |
| ATTGACCGCGATTTCAATCG+TGG | - | tig0020603:86183-86202 | Msa1383560:CDS | 45.0% | |
| ! | GGAAGACCAACTTGTTTAGC+TGG | + | tig0020603:86240-86259 | None:intergenic | 45.0% |
| !! | ACAAGTTGGTCTTCCTGTTG+GGG | - | tig0020603:86245-86264 | Msa1383560:CDS | 45.0% |
| ! | AGACGTGTTAGGAGGAGTAT+CGG | + | tig0020603:86286-86305 | None:intergenic | 45.0% |
| TCTGCAACTTCTACAGTCCA+AGG | + | tig0020603:86405-86424 | None:intergenic | 45.0% | |
| GGTAGGTTTGCCACATAAAC+TGG | - | tig0020603:86548-86567 | Msa1383560:CDS | 45.0% | |
| !! | GCAAGGGTATTTGCCATGCA+AGG | - | tig0020603:86105-86124 | Msa1383560:CDS | 50.0% |
| GGTATTTGCCATGCAAGGTG+TGG | - | tig0020603:86110-86129 | Msa1383560:CDS | 50.0% | |
| GAAAATTGCAGCGTTGCCTG+CGG | + | tig0020603:86154-86173 | None:intergenic | 50.0% | |
| AGCGACCACGATTGAAATCG+CGG | + | tig0020603:86191-86210 | None:intergenic | 50.0% | |
| TTGAAGGTTTCCACCCCAAC+AGG | + | tig0020603:86261-86280 | None:intergenic | 50.0% | |
| GAGTGTTCTCTCTCGATCTG+CGG | - | tig0020603:86461-86480 | Msa1383560:CDS | 50.0% | |
| AGCAACATGCAACTCACACC+AGG | - | tig0020603:86496-86515 | Msa1383560:CDS | 50.0% | |
| GCAACATGCAACTCACACCA+GGG | - | tig0020603:86497-86516 | Msa1383560:CDS | 50.0% | |
| GGTGACATCCACACCTTGCA+TGG | + | tig0020603:86121-86140 | None:intergenic | 55.0% | |
| GTCACCATAATCACCACCGC+AGG | - | tig0020603:86135-86154 | Msa1383560:CDS | 55.0% | |
| GTTGCCTGCGGTGGTGATTA+TGG | + | tig0020603:86142-86161 | None:intergenic | 55.0% | |
| !! | AGTTGGTCTTCCTGTTGGGG+TGG | - | tig0020603:86248-86267 | Msa1383560:CDS | 55.0% |
| !! | AGGAGGAGTATCGGCGTTGA+AGG | + | tig0020603:86277-86296 | None:intergenic | 55.0% |
| TCGATCTGCGGTTCACACGT+TGG | - | tig0020603:86473-86492 | Msa1383560:CDS | 55.0% | |
| ACATGCAACTCACACCAGGG+TGG | - | tig0020603:86500-86519 | Msa1383560:CDS | 55.0% | |
| ATCGGAATCATGGTCCACCC+TGG | + | tig0020603:86517-86536 | None:intergenic | 55.0% | |
| AATTGCAGCGTTGCCTGCGG+TGG | + | tig0020603:86151-86170 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020603 | gene | 86012 | 86641 | 86012 | ID=Msa1383560;Name=Msa1383560 |
| tig0020603 | mRNA | 86012 | 86641 | 86012 | ID=Msa1383560-mRNA-1;Parent=Msa1383560;Name=Msa1383560-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|209 |
| tig0020603 | exon | 86012 | 86641 | 86012 | ID=Msa1383560-mRNA-1:exon:8628;Parent=Msa1383560-mRNA-1 |
| tig0020603 | CDS | 86012 | 86641 | 86012 | ID=Msa1383560-mRNA-1:cds;Parent=Msa1383560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
