AlfalfaGEDB | Msa0781830

M. sativa cultivar zhongmu-4 / Msa0781830

KEGG: -
GO: GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
PFAM: PF03330.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0781830	XP_003616175.1	97.368	266	7	0	1	266	1	266	0.0	544

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0781830	sp\|Q9M9P0\|EXP13_ARATH	83.628	226	37	0	40	265	39	264	6.19e-145	409

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0781830	G7KFU6	97.368	266	7	0	1	266	1	266	0.0	544

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0756490	Msa0781830	0.800264	1.563489e-48	-8.615850e-47
Msa0781830	Msa0863010	0.801371	9.294676e-49	-8.615850e-47
Msa0781830	Msa1042160	0.806827	6.808897e-50	-8.615850e-47
Msa0781830	Msa1042190	0.804565	2.032413e-49	-8.615850e-47
Msa0781830	Msa1167470	0.802693	4.971351e-49	-8.615850e-47
Msa0781830	Msa1393780	0.817721	2.847594e-52	-8.615850e-47
Msa0375640	Msa0781830	0.811443	6.984032e-51	-8.615850e-47
Msa0424160	Msa0781830	0.816754	4.698909e-52	-8.615850e-47
Msa0426530	Msa0781830	0.801608	8.309269e-49	-8.615850e-47
Msa0472950	Msa0781830	0.802644	5.088453e-49	-8.615850e-47
Msa0515800	Msa0781830	0.813775	2.158351e-51	-8.615850e-47
Msa0517250	Msa0781830	0.808399	3.156900e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0781830	MtrunA17_Chr5g0433841	97.368	266	7	0	1	266	1	266	0.0	544
Msa0781830	MtrunA17_Chr3g0109801	68.846	260	68	5	18	264	25	284	3.11e-128	365
Msa0781830	MtrunA17_Chr4g0021561	53.962	265	105	2	1	264	8	256	4.26e-99	290
Msa0781830	MtrunA17_Chr5g0435311	56.000	225	98	1	41	264	82	306	4.42e-91	271
Msa0781830	MtrunA17_Chr5g0417701	56.696	224	96	1	41	264	25	247	2.20e-90	268
Msa0781830	MtrunA17_Chr4g0042521	55.357	224	99	1	41	264	25	247	2.74e-89	265
Msa0781830	MtrunA17_Chr3g0092071	56.250	224	97	1	41	264	25	247	7.46e-89	264
Msa0781830	MtrunA17_Chr7g0238681	49.805	257	124	2	9	264	4	256	1.89e-88	263
Msa0781830	MtrunA17_Chr3g0128801	54.018	224	102	1	41	264	31	253	5.39e-88	262
Msa0781830	MtrunA17_Chr3g0128791	54.018	224	102	1	41	264	31	253	1.44e-87	261
Msa0781830	MtrunA17_Chr2g0328261	54.667	225	101	1	41	264	34	258	2.40e-87	260
Msa0781830	MtrunA17_Chr5g0432611	53.947	228	104	1	37	264	25	251	2.68e-87	260
Msa0781830	MtrunA17_Chr3g0107311	54.911	224	100	1	41	264	32	254	1.14e-86	258
Msa0781830	MtrunA17_Chr7g0217661	52.863	227	98	2	40	266	25	242	1.38e-85	255
Msa0781830	MtrunA17_Chr3g0107291	52.361	233	110	1	32	264	29	260	3.14e-85	255
Msa0781830	MtrunA17_Chr4g0031621	55.310	226	100	1	40	264	31	256	1.88e-81	245
Msa0781830	MtrunA17_Chr7g0218751	52.679	224	103	2	40	262	27	248	2.46e-80	242
Msa0781830	MtrunA17_Chr8g0360041	51.101	227	108	2	36	261	26	250	1.46e-78	238
Msa0781830	MtrunA17_Chr1g0193631	50.667	225	108	2	41	262	30	254	6.30e-77	234
Msa0781830	MtrunA17_Chr7g0218761	54.412	204	90	2	60	262	8	209	6.80e-76	229
Msa0781830	MtrunA17_Chr4g0069711	43.525	278	138	5	3	263	8	283	8.05e-76	232
Msa0781830	MtrunA17_Chr7g0218741	51.327	226	106	3	40	263	28	251	1.95e-75	230
Msa0781830	MtrunA17_Chr6g0453341	53.097	226	97	2	41	266	38	254	2.00e-74	227
Msa0781830	MtrunA17_Chr1g0157061	50.000	212	104	1	53	262	27	238	2.07e-73	224
Msa0781830	MtrunA17_Chr7g0271001	47.982	223	113	2	41	260	27	249	1.10e-71	220
Msa0781830	MtrunA17_Chr8g0360721	52.475	202	93	2	60	260	17	216	6.15e-71	217
Msa0781830	MtrunA17_Chr5g0398271	45.333	225	122	1	39	262	35	259	1.18e-66	207
Msa0781830	MtrunA17_Chr4g0057091	45.852	229	119	2	33	260	32	256	9.12e-64	200
Msa0781830	MtrunA17_Chr2g0324181	66.337	101	34	0	164	264	17	117	5.39e-46	150
Msa0781830	MtrunA17_Chr2g0324191	42.424	165	79	2	1	164	3	152	5.23e-41	138
Msa0781830	MtrunA17_Chr4g0022841	53.043	115	54	0	147	261	2	116	2.14e-37	128
Msa0781830	MtrunA17_Chr4g0022851	51.456	103	45	2	36	137	27	125	7.26e-29	106
Msa0781830	MtrunA17_Chr2g0324221	28.390	236	144	7	39	262	33	255	1.90e-23	95.9
Msa0781830	MtrunA17_Chr2g0324201	29.362	235	141	8	40	262	32	253	2.19e-22	93.2
Msa0781830	MtrunA17_Chr4g0021641	30.213	235	139	8	40	262	40	261	2.30e-22	93.2
Msa0781830	MtrunA17_Chr4g0012801	30.833	240	124	11	41	260	42	259	5.67e-22	92.0
Msa0781830	MtrunA17_Chr8g0359971	53.947	76	34	1	186	260	2	77	7.78e-21	84.0
Msa0781830	MtrunA17_Chr7g0231221	29.565	230	133	11	46	259	53	269	2.28e-18	82.4
Msa0781830	MtrunA17_Chr7g0231211	28.755	233	147	10	37	260	43	265	1.19e-17	80.5
Msa0781830	MtrunA17_Chr8g0359981	44.828	87	36	4	83	164	2	81	7.00e-16	72.0
Msa0781830	MtrunA17_Chr4g0038741	26.724	232	136	9	44	260	26	238	2.75e-13	68.2
Msa0781830	MtrunA17_Chr1g0193571	52.727	55	26	0	208	262	3	57	3.05e-12	60.5
Msa0781830	MtrunA17_Chr5g0399811	27.273	231	149	10	44	265	32	252	7.31e-12	63.9
Msa0781830	MtrunA17_Chr4g0055561	26.695	236	150	9	34	260	24	245	9.25e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0781830	AT3G03220.1	83.628	226	37	0	40	265	39	264	6.29e-146	409
Msa0781830	AT5G56320.1	58.036	224	93	1	41	264	29	251	2.73e-93	276
Msa0781830	AT5G56320.2	58.036	224	93	1	41	264	29	251	2.73e-93	276
Msa0781830	AT5G56320.3	58.036	224	93	1	41	264	29	251	7.83e-93	276
Msa0781830	AT3G55500.1	56.637	226	97	1	40	264	33	258	2.99e-91	271
Msa0781830	AT5G05290.1	57.778	225	93	2	41	264	32	255	4.66e-91	270
Msa0781830	AT2G39700.1	50.758	264	114	2	2	264	7	255	5.56e-91	270
Msa0781830	AT5G02260.1	54.667	225	101	1	41	264	32	256	4.72e-90	267
Msa0781830	AT1G69530.2	51.136	264	114	3	1	264	1	249	4.91e-90	267
Msa0781830	AT1G69530.1	51.136	264	114	3	1	264	1	249	4.91e-90	267
Msa0781830	AT2G37640.1	56.000	225	98	1	41	264	36	260	1.07e-89	267
Msa0781830	AT3G29030.1	54.867	226	94	1	41	266	38	255	1.58e-88	263
Msa0781830	AT2G03090.1	56.250	224	97	1	41	264	30	252	2.61e-88	263
Msa0781830	AT1G69530.3	51.351	259	111	3	1	259	1	244	6.53e-88	263
Msa0781830	AT2G40610.1	52.444	225	105	2	41	264	30	253	1.03e-84	254
Msa0781830	AT1G26770.1	55.357	224	99	1	41	264	26	248	1.73e-84	253
Msa0781830	AT1G26770.2	55.357	224	99	1	41	264	36	258	2.59e-84	253
Msa0781830	AT2G37640.2	57.212	208	88	1	58	264	28	235	3.32e-84	252
Msa0781830	AT5G39270.1	51.261	238	113	2	25	262	42	276	2.82e-81	246
Msa0781830	AT2G28950.1	53.778	225	103	1	41	264	31	255	4.01e-81	244
Msa0781830	AT5G39290.1	51.931	233	106	3	30	262	50	276	1.32e-80	244
Msa0781830	AT5G39280.1	52.232	224	104	2	39	262	52	272	1.44e-79	241
Msa0781830	AT5G39300.1	46.970	264	128	4	8	262	13	273	1.68e-79	241
Msa0781830	AT4G38210.1	51.316	228	108	2	40	264	27	254	7.16e-79	239
Msa0781830	AT1G20190.1	49.091	220	110	1	43	260	28	247	1.08e-75	231
Msa0781830	AT1G69530.4	45.455	264	99	4	1	264	1	219	7.70e-73	222
Msa0781830	AT1G62980.1	48.444	225	115	1	39	262	29	253	1.93e-72	223
Msa0781830	AT4G01630.1	49.107	224	109	3	41	260	28	250	2.78e-71	219
Msa0781830	AT1G69530.5	45.560	259	96	4	1	259	1	214	6.21e-71	218
Msa0781830	AT5G39310.1	45.306	245	130	3	19	262	68	309	4.00e-69	216
Msa0781830	AT1G12560.1	42.308	260	133	4	2	260	13	256	8.25e-69	213
Msa0781830	AT5G39260.1	48.444	225	107	3	39	262	43	259	4.93e-66	206
Msa0781830	AT3G15370.1	47.414	232	107	6	41	264	27	251	1.63e-62	197
Msa0781830	AT1G20190.2	48.128	187	95	1	76	260	2	188	2.19e-62	195
Msa0781830	AT3G15370.2	47.907	215	97	6	58	264	74	281	1.01e-57	186
Msa0781830	AT2G20750.1	31.250	256	150	9	22	262	20	264	2.12e-24	99.0
Msa0781830	AT4G28250.1	31.250	240	137	9	37	262	32	257	5.18e-24	97.8
Msa0781830	AT4G28250.2	30.962	239	133	9	37	262	32	251	8.90e-22	91.7
Msa0781830	AT1G65680.1	28.512	242	145	9	34	260	40	268	4.99e-19	84.7
Msa0781830	AT2G20750.2	30.412	194	113	6	22	203	20	203	5.92e-15	72.4
Msa0781830	AT1G65681.1	26.941	219	130	8	57	260	14	217	8.42e-14	69.3
Msa0781830	AT4G17030.1	26.778	239	137	9	40	260	26	244	3.62e-13	67.8
Msa0781830	AT3G60570.2	24.424	217	137	8	59	262	4	206	6.34e-11	60.8
Msa0781830	AT4G28250.4	31.073	177	94	7	37	203	32	190	8.61e-11	60.5

Find 114 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTTCTGTGCGCCGAATTA+TGG	0.120138	5_2:-64464528	Msa0781830:CDS
GGCTTTCAATGGGTCGGAAT+TGG	0.242564	5_2:-64462114	Msa0781830:CDS
GCTTTCAATGGGTCGGAATT+GGG	0.256772	5_2:-64462113	Msa0781830:CDS
GGAAGAACAAAGTGCTTATT+AGG	0.266775	5_2:+64464471	None:intergenic
TTGTCCAAAACTCCAGTCTT+TGG	0.295901	5_2:+64461991	None:intergenic
GCTCCCAAAGACTGGAGTTT+TGG	0.308877	5_2:-64461995	Msa0781830:CDS
ATTTCTGTGCGCCGAATTAT+GGG	0.321870	5_2:-64464527	Msa0781830:CDS
GTAAGTAGACCTATAATATT+GGG	0.325816	5_2:+64461868	None:intergenic
GGTAAGTAGACCTATAATAT+TGG	0.326700	5_2:+64461867	None:intergenic
AACTTTCCATCTATTATCTT+AGG	0.332869	5_2:-64461742	Msa0781830:three_prime_UTR
ACGCGTTTATATGCCAGTTT+TGG	0.342695	5_2:+64462089	None:intergenic
CATTGTTGCGGTGAAGGTTA+AGG	0.343774	5_2:-64462152	Msa0781830:CDS
GATTTAAAGTGGTGTATTCC+TGG	0.357927	5_2:-64464576	Msa0781830:CDS
TGTCCAAAACTCCAGTCTTT+GGG	0.365496	5_2:+64461992	None:intergenic
GTCGGAATTGGGGCCAAAAC+TGG	0.376301	5_2:-64462102	Msa0781830:CDS
TGCGCCGAATTATGGGTTTA+CGG	0.384118	5_2:-64464520	Msa0781830:CDS
AAGAACAGGTTGGCTTTCAA+TGG	0.397007	5_2:-64462125	Msa0781830:CDS
GAATCAACCTCTTTCATTTG+AGG	0.401927	5_2:-64462059	Msa0781830:CDS
GTGGCGCGTGCTTTGAATTA+CGG	0.402202	5_2:-64464608	Msa0781830:CDS
CAAACTCCTAAGATAATAGA+TGG	0.403066	5_2:+64461736	None:intergenic
AGAGACTGGTCCCTTGAAAC+AGG	0.404717	5_2:+64461920	None:intergenic
TATGAAAATATATACCTTGT+TGG	0.406110	5_2:-64461710	Msa0781830:three_prime_UTR
GCAATGGGTGGGTGGGACAC+TGG	0.410370	5_2:+64465030	None:intergenic
AATTATGGGTTTACGGTTGA+AGG	0.412698	5_2:-64464513	Msa0781830:CDS
ATTGTTGCGGTGAAGGTTAA+GGG	0.417256	5_2:-64462151	Msa0781830:CDS
AACAGTTGCCATGCCGTATC+CGG	0.417973	5_2:+64464668	None:intergenic
AGAGTAGTTGATAATTGATT+TGG	0.424966	5_2:-64461795	Msa0781830:three_prime_UTR
CGATTTACAGTCACCGGATC+TGG	0.430139	5_2:-64462220	Msa0781830:CDS
GAGAAAATGAAGAAAGAAAG+TGG	0.437740	5_2:+64464963	None:intergenic
AATATTATAGGTCTACTTAC+CGG	0.448816	5_2:-64461865	Msa0781830:three_prime_UTR
ATCAAATTGCTTGCCTTCAA+AGG	0.451831	5_2:+64461967	None:intergenic
ACTGTGTCACGTGGATCTAC+CGG	0.453305	5_2:+64464726	None:intergenic
GTCGGCGGCGCGTGTGGCTA+CGG	0.454653	5_2:-64464705	Msa0781830:CDS
GTGCTGATCAGCAATGTTGC+TGG	0.456538	5_2:-64462184	Msa0781830:CDS
CAGATCCGGTGACTGTAAAT+CGG	0.457752	5_2:+64462221	None:intergenic
AACGAAGAAAACCTGTTTCA+AGG	0.458176	5_2:-64461931	Msa0781830:three_prime_UTR
GACTTATCAAAAGCCGGATA+CGG	0.459622	5_2:-64464681	Msa0781830:CDS
GTAGTTGATAATTGATTTGG+TGG	0.461348	5_2:-64461792	Msa0781830:three_prime_UTR
GTAACAATAATCGAAGTTCC+AGG	0.466240	5_2:+64464558	None:intergenic
AAGATCGCTATTTGGAAAGC+TGG	0.469148	5_2:-64464432	Msa0781830:CDS
GGGTTTACGGTTGAAGGTGG+TGG	0.469331	5_2:-64464507	Msa0781830:CDS
CTTTCAATGGGTCGGAATTG+GGG	0.469604	5_2:-64462112	Msa0781830:CDS
AAGGTTAAGGGCTCAAGAAC+AGG	0.472972	5_2:-64462139	Msa0781830:CDS
ATCAGCAATGTTGCTGGCCA+TGG	0.474378	5_2:-64462178	Msa0781830:CDS
TGTTGTTGCAATGGGTGGGT+GGG	0.481326	5_2:+64465023	None:intergenic
CTTCCCCTTCTTTCACCGAA+TGG	0.483052	5_2:-64464776	Msa0781830:CDS
ATCTTCTCAAAAGCATCAAT+AGG	0.486912	5_2:+64464450	None:intergenic
GGCACGTGCGGATCGCCATT+CGG	0.487063	5_2:+64464761	None:intergenic
TTGTTGTTGCAATGGGTGGG+TGG	0.487519	5_2:+64465022	None:intergenic
ACAATGTTGCTCCCAAAGAC+TGG	0.490580	5_2:-64462003	Msa0781830:CDS
AGAACAGGTTGGCTTTCAAT+GGG	0.491999	5_2:-64462124	Msa0781830:CDS
AGAAGAGTAGTGTGAGGTGA+CGG	0.492028	5_2:+64464830	None:intergenic
TGGAGACATTGTTGCGGTGA+AGG	0.493493	5_2:-64462158	Msa0781830:CDS
TGGATCTACCGGGGCGTAGT+AGG	0.498940	5_2:+64464737	None:intergenic
TTTGAGGTTACGAGTAGTGA+TGG	0.501425	5_2:-64462043	Msa0781830:CDS
CTCGTAACCTCAAATGAAAG+AGG	0.502745	5_2:+64462052	None:intergenic
GATCGCCATTCGGTGAAAGA+AGG	0.504459	5_2:+64464771	None:intergenic
TCAACCGTAAACCCATAATT+CGG	0.504889	5_2:+64464516	None:intergenic
GGGGAAGATTGTTGAGAAGG+TGG	0.507413	5_2:+64464792	None:intergenic
AGAAGGTGGTGGTTGAGAAG+AGG	0.512505	5_2:+64464806	None:intergenic
GAAGGGGAAGATTGTTGAGA+AGG	0.515350	5_2:+64464789	None:intergenic
CTGTGTCACGTGGATCTACC+GGG	0.516254	5_2:+64464727	None:intergenic
ACGAAGAAAACCTGTTTCAA+GGG	0.518898	5_2:-64461930	Msa0781830:three_prime_UTR
AGGATAAAGTGCAGAAGGGA+AGG	0.530862	5_2:-64462250	Msa0781830:CDS
TGAAGAAGAAAGAAAAGTGA+GGG	0.531579	5_2:+64464919	None:intergenic
TATGGGTTTACGGTTGAAGG+TGG	0.532788	5_2:-64464510	Msa0781830:CDS
ATCGCCATTCGGTGAAAGAA+GGG	0.538930	5_2:+64464772	None:intergenic
TACGGAGACTTATCAAAAGC+CGG	0.550504	5_2:-64464687	Msa0781830:CDS
GACACAGTCGGCGGCGCGTG+TGG	0.550703	5_2:-64464711	Msa0781830:CDS
CAGGTTGGCTTTCAATGGGT+CGG	0.553172	5_2:-64462120	Msa0781830:CDS
ATGAAGAAGAAAGAAAAGTG+AGG	0.555383	5_2:+64464918	None:intergenic
GGAGCAGGATAAAGTGCAGA+AGG	0.570200	5_2:-64462255	Msa0781830:intron
GGAGTCCGATTTACAGTCAC+CGG	0.573922	5_2:-64462226	Msa0781830:CDS
ATAAAGTGCAGAAGGGAAGG+TGG	0.575254	5_2:-64462247	Msa0781830:CDS
TGAAATGAAGATACCAGATC+CGG	0.576855	5_2:+64462207	None:intergenic
AGAACAAAGTGCTTATTAGG+AGG	0.582144	5_2:+64464474	None:intergenic
TTGTTGGTCAACAACAAAAG+AGG	0.583248	5_2:-64461694	Msa0781830:three_prime_UTR
TTGAGGTTACGAGTAGTGAT+GGG	0.583484	5_2:-64462042	Msa0781830:CDS
TTTGAACGAGGTCAGATCTG+TGG	0.583854	5_2:-64464627	Msa0781830:CDS
AATTAACATGTAAATGCTTG+AGG	0.586547	5_2:-64461671	Msa0781830:three_prime_UTR
AGAGGAAGAAGAGTAGTGTG+AGG	0.594007	5_2:+64464824	None:intergenic
ATCAAAAGCCGGATACGGCA+TGG	0.595287	5_2:-64464676	Msa0781830:CDS
AATGGGAAAGGGAAAGAGAC+TGG	0.597600	5_2:+64461906	None:intergenic
ATTATACCTACGATACTGAA+GGG	0.605648	5_2:+64464401	None:intergenic
GAAAGTGGTAGTGAAAGTGA+TGG	0.609379	5_2:+64464978	None:intergenic
TATTATACCTACGATACTGA+AGG	0.610443	5_2:+64464400	None:intergenic
GAAGATTGTTGAGAAGGTGG+TGG	0.611367	5_2:+64464795	None:intergenic
AAAGTGGTAGTGAAAGTGAT+GGG	0.615535	5_2:+64464979	None:intergenic
GGCGTAGTAGGTGGCACGTG+CGG	0.618083	5_2:+64464749	None:intergenic
GTAACATTTCCTCAGTGTGA+AGG	0.620175	5_2:+64464892	None:intergenic
ATCTACCGGGGCGTAGTAGG+TGG	0.621184	5_2:+64464740	None:intergenic
ACATGCCCCTTCAGTATCGT+AGG	0.621276	5_2:-64464407	Msa0781830:intron
TCACCGCAACAATGTCTCCA+TGG	0.621449	5_2:+64462161	None:intergenic
GGATACGGCATGGCAACTGT+TGG	0.621514	5_2:-64464666	Msa0781830:CDS
TTGTAAGATTGCCAATGTGA+TGG	0.622247	5_2:-64461768	Msa0781830:three_prime_UTR
TTAAGGGCTCAAGAACAGGT+TGG	0.623161	5_2:-64462135	Msa0781830:CDS
GCGCCGCCGACTGTGTCACG+TGG	0.625743	5_2:+64464717	None:intergenic
GGTGTGTGGATGATTTAAAG+TGG	0.628924	5_2:-64464587	Msa0781830:CDS
TCGCCATTCGGTGAAAGAAG+GGG	0.643549	5_2:+64464773	None:intergenic
AAAATGTAAGAGAACACCAC+CGG	0.649513	5_2:+64461846	None:intergenic
GAGCAGGATAAAGTGCAGAA+GGG	0.649937	5_2:-64462254	Msa0781830:intron
GTGCTTTGAATTACGGTGTG+TGG	0.652130	5_2:-64464601	Msa0781830:CDS
GGAAAGTTACACCATCACAT+TGG	0.657775	5_2:+64461757	None:intergenic
AGTGAAGCTTTGTTTGAACG+AGG	0.664292	5_2:-64464639	Msa0781830:CDS
CTTCATTTACCTTCACACTG+AGG	0.670406	5_2:-64464901	Msa0781830:five_prime_UTR
TAGTGATGGGAAAACAGTCA+CGG	0.670779	5_2:-64462029	Msa0781830:CDS
TTATACCTACGATACTGAAG+GGG	0.678752	5_2:+64464402	None:intergenic
ACGTGCCACCTACTACGCCC+CGG	0.682530	5_2:-64464745	Msa0781830:CDS
GTAGATCCACGTGACACAGT+CGG	0.684257	5_2:-64464723	Msa0781830:CDS
TAAGTAGACCTATAATATTG+GGG	0.687551	5_2:+64461869	None:intergenic
TGGCCATGGAGACATTGTTG+CGG	0.694232	5_2:-64462164	Msa0781830:CDS
ATTATAGGTCTACTTACCGG+TGG	0.694910	5_2:-64461862	Msa0781830:three_prime_UTR
TGTGTCACGTGGATCTACCG+GGG	0.729868	5_2:+64464728	None:intergenic
GATCCACGTGACACAGTCGG+CGG	0.742025	5_2:-64464720	Msa0781830:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTAGTCAAATTTAATGTT+TGG	-	chr5_2:64462673-64462692	Msa0781830:intron	15.0%
!!	ATTAAGCTTATTGAATTATA+TGG	-	chr5_2:64462802-64462821	Msa0781830:intron	15.0%
!!	AAATCAAAAATTGTATTTGA+GGG	+	chr5_2:64463700-64463719	None:intergenic	15.0%
!!!	TACTTAAAAAAACTTGATTT+TGG	-	chr5_2:64464379-64464398	Msa0781830:intron	15.0%
!!!	CTCTTACATTTTTATTATTT+TGG	-	chr5_2:64464856-64464875	Msa0781830:CDS	15.0%
!!!	TTATTTTTTGTTGTTGCAAT+GGG	+	chr5_2:64461679-64461698	None:intergenic	20.0%
!!!	TTTATTTTTTGTTGTTGCAA+TGG	+	chr5_2:64461680-64461699	None:intergenic	20.0%
!!	TTTAATAGTATACTTGAGTT+TGG	-	chr5_2:64462722-64462741	Msa0781830:intron	20.0%
!!!	TTGTTAATGGTTAATTTGTT+AGG	-	chr5_2:64463244-64463263	Msa0781830:intron	20.0%
!!!	TGTTAATGGTTAATTTGTTA+GGG	-	chr5_2:64463245-64463264	Msa0781830:intron	20.0%
!!	GAAATCAAAAATTGTATTTG+AGG	+	chr5_2:64463701-64463720	None:intergenic	20.0%
!!	ATACAAAATCCCATTAATTT+GGG	+	chr5_2:64463725-64463744	None:intergenic	20.0%
!!	AATACAAAATCCCATTAATT+TGG	+	chr5_2:64463726-64463745	None:intergenic	20.0%
!!	TACTAATTGAATTCTATGTA+TGG	+	chr5_2:64464001-64464020	None:intergenic	20.0%
!!	ATTAACATTGTATGAAGATA+CGG	+	chr5_2:64464028-64464047	None:intergenic	20.0%
!!!	TTATTAGACCATTAATTTTG+CGG	+	chr5_2:64464201-64464220	None:intergenic	20.0%
!!	AAAAAAATTGAAATGGGAAA+GGG	+	chr5_2:64464799-64464818	None:intergenic	20.0%
!!	AAAAAAAATTGAAATGGGAA+AGG	+	chr5_2:64464800-64464819	None:intergenic	20.0%
!!	GGGTAAAAAAAAATTGAAAT+GGG	+	chr5_2:64464805-64464824	None:intergenic	20.0%
!!!	TTTTTTTACCCCAATATTAT+AGG	-	chr5_2:64464814-64464833	Msa0781830:CDS	20.0%
!!	TATGAAAATATATACCTTGT+TGG	-	chr5_2:64464981-64465000	Msa0781830:five_prime_UTR	20.0%
!!!	GGTTATTTCTATTTTTCCTA+TGG	-	chr5_2:64462759-64462778	Msa0781830:intron	25.0%
!!!	TTTTCAAGAAGATATTCCAT+AGG	+	chr5_2:64462778-64462797	None:intergenic	25.0%
!	AAGCTTAAAAACGAAGTTAT+AGG	-	chr5_2:64462850-64462869	Msa0781830:intron	25.0%
!	ATTACTAAAACTAGCATTCA+AGG	+	chr5_2:64462989-64463008	None:intergenic	25.0%
!!!	GAATGCTAGTTTTAGTAATA+AGG	-	chr5_2:64462990-64463009	Msa0781830:intron	25.0%
!	ACACAAATTCCATTAAACTT+GGG	+	chr5_2:64463177-64463196	None:intergenic	25.0%
!	AACACAAATTCCATTAAACT+TGG	+	chr5_2:64463178-64463197	None:intergenic	25.0%
!!!	GTTAATGGTTAATTTGTTAG+GGG	-	chr5_2:64463246-64463265	Msa0781830:intron	25.0%
!!!	TTAATGGTTAATTTGTTAGG+GGG	-	chr5_2:64463247-64463266	Msa0781830:intron	25.0%
!	TAATGAAATTCAAGTAGCAA+AGG	-	chr5_2:64463440-64463459	Msa0781830:intron	25.0%
!	AATGAAATTCAAGTAGCAAA+GGG	-	chr5_2:64463441-64463460	Msa0781830:intron	25.0%
!!	AAATTGTATTTGAGGGTTTT+GGG	+	chr5_2:64463693-64463712	None:intergenic	25.0%
!!	AAAATTGTATTTGAGGGTTT+TGG	+	chr5_2:64463694-64463713	None:intergenic	25.0%
!!	TACAAAATCCCATTAATTTG+GGG	+	chr5_2:64463724-64463743	None:intergenic	25.0%
!	AACAAATCCTAAAGGAAAAT+AGG	-	chr5_2:64463861-64463880	Msa0781830:intron	25.0%
!	TCTATGTATGGAAAAATGTT+GGG	+	chr5_2:64463989-64464008	None:intergenic	25.0%
!	TTCTATGTATGGAAAAATGT+TGG	+	chr5_2:64463990-64464009	None:intergenic	25.0%
!	GTATCTTCATACAATGTTAA+TGG	-	chr5_2:64464027-64464046	Msa0781830:intron	25.0%
!	GGGGTAAAAAAAAATTGAAA+TGG	+	chr5_2:64464806-64464825	None:intergenic	25.0%
!	TAAGTAGACCTATAATATTG+GGG	+	chr5_2:64464825-64464844	None:intergenic	25.0%
!	GTAAGTAGACCTATAATATT+GGG	+	chr5_2:64464826-64464845	None:intergenic	25.0%
!	AATATTATAGGTCTACTTAC+CGG	-	chr5_2:64464826-64464845	Msa0781830:CDS	25.0%
!!!	AGAGTAGTTGATAATTGATT+TGG	-	chr5_2:64464896-64464915	Msa0781830:exon	25.0%
!	AACTTTCCATCTATTATCTT+AGG	-	chr5_2:64464949-64464968	Msa0781830:five_prime_UTR	25.0%
!	AATTAACATGTAAATGCTTG+AGG	-	chr5_2:64465020-64465039	Msa0781830:five_prime_UTR	25.0%
	GAGAAAATGAAGAAAGAAAG+TGG	+	chr5_2:64461731-64461750	None:intergenic	30.0%
	TGAAGAAGAAAGAAAAGTGA+GGG	+	chr5_2:64461775-64461794	None:intergenic	30.0%
	ATGAAGAAGAAAGAAAAGTG+AGG	+	chr5_2:64461776-64461795	None:intergenic	30.0%
!!	ATCTTCTCAAAAGCATCAAT+AGG	+	chr5_2:64462244-64462263	None:intergenic	30.0%
	ATTATACCTACGATACTGAA+GGG	+	chr5_2:64462293-64462312	None:intergenic	30.0%
	TATTATACCTACGATACTGA+AGG	+	chr5_2:64462294-64462313	None:intergenic	30.0%
	ACATCTTCAATCATAAGTGT+CGG	-	chr5_2:64462574-64462593	Msa0781830:intron	30.0%
	TTAACTGTGACAAAACTTCT+AGG	-	chr5_2:64462613-64462632	Msa0781830:intron	30.0%
!	TCTATCAAAATCTTGGTAGT+TGG	-	chr5_2:64463048-64463067	Msa0781830:intron	30.0%
	AGAGTTTATCCCAAGTTTAA+TGG	-	chr5_2:64463165-64463184	Msa0781830:intron	30.0%
!	TGGAATTTGTGTTTTGTCAT+TGG	-	chr5_2:64463185-64463204	Msa0781830:intron	30.0%
!!	TTTGTCATTGGCTTCTTAAT+TGG	-	chr5_2:64463197-64463216	Msa0781830:intron	30.0%
	GCTGAGAAACAAATTGTTAA+TGG	-	chr5_2:64463231-64463250	Msa0781830:intron	30.0%
	ACTTGAATTTCATTAGCACT+AGG	+	chr5_2:64463435-64463454	None:intergenic	30.0%
!	AATTGTATTTGAGGGTTTTG+GGG	+	chr5_2:64463692-64463711	None:intergenic	30.0%
!	TTTGATTTCCCCCAAATTAA+TGG	-	chr5_2:64463712-64463731	Msa0781830:intron	30.0%
	TTGATTTCCCCCAAATTAAT+GGG	-	chr5_2:64463713-64463732	Msa0781830:intron	30.0%
!	ACAAAATCCCATTAATTTGG+GGG	+	chr5_2:64463723-64463742	None:intergenic	30.0%
!	CTACTATCCTATTTTCCTTT+AGG	+	chr5_2:64463871-64463890	None:intergenic	30.0%
!	ATATTTTGCTCCATTATGAG+TGG	-	chr5_2:64463925-64463944	Msa0781830:intron	30.0%
	AATTCCTGTAGATAATGAGA+AGG	-	chr5_2:64464066-64464085	Msa0781830:intron	30.0%
	ATTCCTGTAGATAATGAGAA+GGG	-	chr5_2:64464067-64464086	Msa0781830:intron	30.0%
!	ATTTTGCTAGCAAGTACATT+AGG	-	chr5_2:64464107-64464126	Msa0781830:intron	30.0%
!	TTTTGCTAGCAAGTACATTA+GGG	-	chr5_2:64464108-64464127	Msa0781830:intron	30.0%
	AGAAAAGACCGCAAAATTAA+TGG	-	chr5_2:64464190-64464209	Msa0781830:intron	30.0%
	TATGCTACATCATTTACTGT+TGG	-	chr5_2:64464326-64464345	Msa0781830:intron	30.0%
	TTTGAATGTTGTGTTCTGTT+TGG	-	chr5_2:64464415-64464434	Msa0781830:CDS	30.0%
	GGTAAGTAGACCTATAATAT+TGG	+	chr5_2:64464827-64464846	None:intergenic	30.0%
!!	GTAGTTGATAATTGATTTGG+TGG	-	chr5_2:64464899-64464918	Msa0781830:five_prime_UTR	30.0%
	CAAACTCCTAAGATAATAGA+TGG	+	chr5_2:64464958-64464977	None:intergenic	30.0%
!!!	TTTTTGTTGTTGCAATGGGT+GGG	+	chr5_2:64461675-64461694	None:intergenic	35.0%
!!!	TTTTTTGTTGTTGCAATGGG+TGG	+	chr5_2:64461676-64461695	None:intergenic	35.0%
!	AAAGTGGTAGTGAAAGTGAT+GGG	+	chr5_2:64461715-64461734	None:intergenic	35.0%
!	GATTTAAAGTGGTGTATTCC+TGG	-	chr5_2:64462115-64462134	Msa0781830:CDS	35.0%
	GTAACAATAATCGAAGTTCC+AGG	+	chr5_2:64462136-64462155	None:intergenic	35.0%
	TCAACCGTAAACCCATAATT+CGG	+	chr5_2:64462178-64462197	None:intergenic	35.0%
	AATTATGGGTTTACGGTTGA+AGG	-	chr5_2:64462178-64462197	Msa0781830:CDS	35.0%
!	AGAACAAAGTGCTTATTAGG+AGG	+	chr5_2:64462220-64462239	None:intergenic	35.0%
!	GGAAGAACAAAGTGCTTATT+AGG	+	chr5_2:64462223-64462242	None:intergenic	35.0%
!!	CTTTTGAGAAGATCGCTATT+TGG	-	chr5_2:64462251-64462270	Msa0781830:CDS	35.0%
	TTATACCTACGATACTGAAG+GGG	+	chr5_2:64462292-64462311	None:intergenic	35.0%
	TTCAATGTAATCGCATGTGT+TGG	-	chr5_2:64462518-64462537	Msa0781830:intron	35.0%
!!	AGTTTGGCTTGTTTATGAGT+TGG	-	chr5_2:64462738-64462757	Msa0781830:intron	35.0%
!	GAGTAAGAGAAGAGTCTTTT+AGG	-	chr5_2:64462872-64462891	Msa0781830:intron	35.0%
!	GGATAAAGTATTATACAGCC+AGG	-	chr5_2:64463011-64463030	Msa0781830:intron	35.0%
!!	ACCAAGATTTTGATAGACAG+TGG	+	chr5_2:64463045-64463064	None:intergenic	35.0%
	CTTCATGCATAATGCTCATT+TGG	-	chr5_2:64463296-64463315	Msa0781830:intron	35.0%
	GCATAAAGTTCCAAAGAGAA+AGG	+	chr5_2:64463365-64463384	None:intergenic	35.0%
	GAATTTCATTAGCACTAGGA+AGG	+	chr5_2:64463431-64463450	None:intergenic	35.0%
	TTTGAGGGATGGGAAATATA+GGG	-	chr5_2:64463529-64463548	Msa0781830:intron	35.0%
!!	ATTGTATTTGAGGGTTTTGG+GGG	+	chr5_2:64463691-64463710	None:intergenic	35.0%
	TGGAAGAAATAGTAGGCTTT+GGG	+	chr5_2:64463782-64463801	None:intergenic	35.0%
	GAACAAGTGGAAGAAATAGT+AGG	+	chr5_2:64463789-64463808	None:intergenic	35.0%
!	TTAGGATTTGTTTGCGAGTT+TGG	+	chr5_2:64463853-64463872	None:intergenic	35.0%
	AACTCGCAAACAAATCCTAA+AGG	-	chr5_2:64463853-64463872	Msa0781830:intron	35.0%
	CTATTAACCCACGGTTTAAA+TGG	+	chr5_2:64464242-64464261	None:intergenic	35.0%
	ATAGCTGATAAAGGAACTTG+TGG	-	chr5_2:64464268-64464287	Msa0781830:intron	35.0%
	TGAAATGAAGATACCAGATC+CGG	+	chr5_2:64464487-64464506	None:intergenic	35.0%
	GAATCAACCTCTTTCATTTG+AGG	-	chr5_2:64464632-64464651	Msa0781830:CDS	35.0%
!!!	AGTTTTGGACAAACCTTTGA+AGG	-	chr5_2:64464711-64464730	Msa0781830:CDS	35.0%
	ATCAAATTGCTTGCCTTCAA+AGG	+	chr5_2:64464727-64464746	None:intergenic	35.0%
!	TGAAACAGGTTTTCTTCGTT+TGG	+	chr5_2:64464760-64464779	None:intergenic	35.0%
	AACGAAGAAAACCTGTTTCA+AGG	-	chr5_2:64464760-64464779	Msa0781830:CDS	35.0%
	ACGAAGAAAACCTGTTTCAA+GGG	-	chr5_2:64464761-64464780	Msa0781830:CDS	35.0%
	AAAATGTAAGAGAACACCAC+CGG	+	chr5_2:64464848-64464867	None:intergenic	35.0%
	TTGTAAGATTGCCAATGTGA+TGG	-	chr5_2:64464923-64464942	Msa0781830:five_prime_UTR	35.0%
!!!	TCTTTTGTTGTTGACCAACA+AGG	+	chr5_2:64464998-64465017	None:intergenic	35.0%
	TTGTTGGTCAACAACAAAAG+AGG	-	chr5_2:64464997-64465016	Msa0781830:five_prime_UTR	35.0%
!	GAAAGTGGTAGTGAAAGTGA+TGG	+	chr5_2:64461716-64461735	None:intergenic	40.0%
	CTTCATTTACCTTCACACTG+AGG	-	chr5_2:64461790-64461809	Msa0781830:three_prime_UTR	40.0%
	GTAACATTTCCTCAGTGTGA+AGG	+	chr5_2:64461802-64461821	None:intergenic	40.0%
	TACGGAGACTTATCAAAAGC+CGG	-	chr5_2:64462004-64462023	Msa0781830:CDS	40.0%
	GACTTATCAAAAGCCGGATA+CGG	-	chr5_2:64462010-64462029	Msa0781830:CDS	40.0%
!!	AGTGAAGCTTTGTTTGAACG+AGG	-	chr5_2:64462052-64462071	Msa0781830:CDS	40.0%
	GGTGTGTGGATGATTTAAAG+TGG	-	chr5_2:64462104-64462123	Msa0781830:CDS	40.0%
	AATTTCTGTGCGCCGAATTA+TGG	-	chr5_2:64462163-64462182	Msa0781830:CDS	40.0%
	ATTTCTGTGCGCCGAATTAT+GGG	-	chr5_2:64462164-64462183	Msa0781830:CDS	40.0%
	AAGATCGCTATTTGGAAAGC+TGG	-	chr5_2:64462259-64462278	Msa0781830:intron	40.0%
!!	ATTTTGATAGACAGTGGCAG+AGG	+	chr5_2:64463039-64463058	None:intergenic	40.0%
	GCCACTGTCTATCAAAATCT+TGG	-	chr5_2:64463041-64463060	Msa0781830:intron	40.0%
!!	GTTAATTTGTTAGGGGGAGA+GGG	-	chr5_2:64463253-64463272	Msa0781830:intron	40.0%
	TCAAGTAGCAAAGGGATGAA+AGG	-	chr5_2:64463449-64463468	Msa0781830:intron	40.0%
	CAAGTAGCAAAGGGATGAAA+GGG	-	chr5_2:64463450-64463469	Msa0781830:intron	40.0%
	AAGGGACTAAATTGACCCTA+AGG	-	chr5_2:64463468-64463487	Msa0781830:intron	40.0%
!!	ATTGACCCTAAGGCTTTGTT+TGG	-	chr5_2:64463478-64463497	Msa0781830:intron	40.0%
!!	TTGACCCTAAGGCTTTGTTT+GGG	-	chr5_2:64463479-64463498	Msa0781830:intron	40.0%
	AACTCCCAAACAAAGCCTTA+GGG	+	chr5_2:64463486-64463505	None:intergenic	40.0%
!	CTTTGAGGGATGGGAAATAT+AGG	-	chr5_2:64463528-64463547	Msa0781830:intron	40.0%
	TTGAGGGATGGGAAATATAG+GGG	-	chr5_2:64463530-64463549	Msa0781830:intron	40.0%
	ATGGGAAATATAGGGGGAAA+TGG	-	chr5_2:64463537-64463556	Msa0781830:intron	40.0%
	GTGGAAGAAATAGTAGGCTT+TGG	+	chr5_2:64463783-64463802	None:intergenic	40.0%
	GGAAAAAAGGAAGGAACAAG+TGG	+	chr5_2:64463802-64463821	None:intergenic	40.0%
	ATTTGTTTGCGAGTTTGGAG+GGG	+	chr5_2:64463848-64463867	None:intergenic	40.0%
!	GATTTGTTTGCGAGTTTGGA+GGG	+	chr5_2:64463849-64463868	None:intergenic	40.0%
	ACATTGTATGAAGATACGGC+TGG	+	chr5_2:64464024-64464043	None:intergenic	40.0%
	GTTCCCTTCTCATTATCTAC+AGG	+	chr5_2:64464073-64464092	None:intergenic	40.0%
!	TTTCGTACACGGTTTGAAAC+GGG	+	chr5_2:64464159-64464178	None:intergenic	40.0%
	GTTTCGTACACGGTTTGAAA+CGG	+	chr5_2:64464160-64464179	None:intergenic	40.0%
	TTTCAAACCGTGTACGAAAC+AGG	-	chr5_2:64464160-64464179	Msa0781830:intron	40.0%
	TATGCTTGGCTATTAACCCA+CGG	+	chr5_2:64464251-64464270	None:intergenic	40.0%
	TAGCCAAGCATAGCTGATAA+AGG	-	chr5_2:64464259-64464278	Msa0781830:intron	40.0%
	GTTCCTTTATCAGCTATGCT+TGG	+	chr5_2:64464265-64464284	None:intergenic	40.0%
	TAGCATAATGAGAGCTGTTG+GGG	+	chr5_2:64464313-64464332	None:intergenic	40.0%
	GTAGCATAATGAGAGCTGTT+GGG	+	chr5_2:64464314-64464333	None:intergenic	40.0%
	TGTAGCATAATGAGAGCTGT+TGG	+	chr5_2:64464315-64464334	None:intergenic	40.0%
	ATTGTTGCGGTGAAGGTTAA+GGG	-	chr5_2:64464540-64464559	Msa0781830:CDS	40.0%
!!	AAGAACAGGTTGGCTTTCAA+TGG	-	chr5_2:64464566-64464585	Msa0781830:CDS	40.0%
!!	AGAACAGGTTGGCTTTCAAT+GGG	-	chr5_2:64464567-64464586	Msa0781830:CDS	40.0%
!	ACGCGTTTATATGCCAGTTT+TGG	+	chr5_2:64464605-64464624	None:intergenic	40.0%
	CTCGTAACCTCAAATGAAAG+AGG	+	chr5_2:64464642-64464661	None:intergenic	40.0%
!	TTTGAGGTTACGAGTAGTGA+TGG	-	chr5_2:64464648-64464667	Msa0781830:CDS	40.0%
	TTGAGGTTACGAGTAGTGAT+GGG	-	chr5_2:64464649-64464668	Msa0781830:CDS	40.0%
!	TAGTGATGGGAAAACAGTCA+CGG	-	chr5_2:64464662-64464681	Msa0781830:CDS	40.0%
	TGTCCAAAACTCCAGTCTTT+GGG	+	chr5_2:64464702-64464721	None:intergenic	40.0%
	TTGTCCAAAACTCCAGTCTT+TGG	+	chr5_2:64464703-64464722	None:intergenic	40.0%
	ATTATAGGTCTACTTACCGG+TGG	-	chr5_2:64464829-64464848	Msa0781830:CDS	40.0%
	GGAAAGTTACACCATCACAT+TGG	+	chr5_2:64464937-64464956	None:intergenic	40.0%
	AGAAGAGTAGTGTGAGGTGA+CGG	+	chr5_2:64461864-64461883	None:intergenic	45.0%
	AGAGGAAGAAGAGTAGTGTG+AGG	+	chr5_2:64461870-64461889	None:intergenic	45.0%
	GAAGATTGTTGAGAAGGTGG+TGG	+	chr5_2:64461899-64461918	None:intergenic	45.0%
	GAAGGGGAAGATTGTTGAGA+AGG	+	chr5_2:64461905-64461924	None:intergenic	45.0%
!	ATCGCCATTCGGTGAAAGAA+GGG	+	chr5_2:64461922-64461941	None:intergenic	45.0%
	TTTGAACGAGGTCAGATCTG+TGG	-	chr5_2:64462064-64462083	Msa0781830:CDS	45.0%
!	GTGCTTTGAATTACGGTGTG+TGG	-	chr5_2:64462090-64462109	Msa0781830:CDS	45.0%
	TGCGCCGAATTATGGGTTTA+CGG	-	chr5_2:64462171-64462190	Msa0781830:CDS	45.0%
	TATGGGTTTACGGTTGAAGG+TGG	-	chr5_2:64462181-64462200	Msa0781830:CDS	45.0%
	TATTATCAGCCTCGACGTGT+GGG	+	chr5_2:64462482-64462501	None:intergenic	45.0%
	TTATTATCAGCCTCGACGTG+TGG	+	chr5_2:64462483-64462502	None:intergenic	45.0%
	TCCAACGGACGAGATTTGAT+TGG	-	chr5_2:64463089-64463108	Msa0781830:intron	45.0%
	CCAACGGACGAGATTTGATT+GGG	-	chr5_2:64463090-64463109	Msa0781830:intron	45.0%
!!	TTTGATTGGGTTGGCTGCAA+AGG	-	chr5_2:64463103-64463122	Msa0781830:intron	45.0%
!!	GGTTAATTTGTTAGGGGGAG+AGG	-	chr5_2:64463252-64463271	Msa0781830:intron	45.0%
!	AAGAGAAAGGTGTGCATCCT+TGG	+	chr5_2:64463352-64463371	None:intergenic	45.0%
	AGGATGCACACCTTTCTCTT+TGG	-	chr5_2:64463352-64463371	Msa0781830:intron	45.0%
	GAACTCCCAAACAAAGCCTT+AGG	+	chr5_2:64463487-64463506	None:intergenic	45.0%
!	CTTTGTTTGGGAGTTCAGAG+AGG	-	chr5_2:64463491-64463510	Msa0781830:intron	45.0%
	TGAGGGATGGGAAATATAGG+GGG	-	chr5_2:64463531-64463550	Msa0781830:intron	45.0%
	GGAAATATAGGGGGAAATGG+AGG	-	chr5_2:64463540-64463559	Msa0781830:intron	45.0%
!!	GAAATAGTAGGCTTTGGGAG+CGG	+	chr5_2:64463777-64463796	None:intergenic	45.0%
!!	GAGGGCTTTGGAAAAAAGGA+AGG	+	chr5_2:64463811-64463830	None:intergenic	45.0%
!	GGATTTGTTTGCGAGTTTGG+AGG	+	chr5_2:64463850-64463869	None:intergenic	45.0%
	CTCCATTATGAGTGGCAAAC+TGG	-	chr5_2:64463933-64463952	Msa0781830:intron	45.0%
	TGCCAGTTTGCCACTCATAA+TGG	+	chr5_2:64463938-64463957	None:intergenic	45.0%
!!	TTTTGCGGTCTTTTCTAGCC+CGG	+	chr5_2:64464186-64464205	None:intergenic	45.0%
	GGTTTAAATGGTGCGAACAC+GGG	+	chr5_2:64464230-64464249	None:intergenic	45.0%
	CGGTTTAAATGGTGCGAACA+CGG	+	chr5_2:64464231-64464250	None:intergenic	45.0%
	TGTTCGCACCATTTAAACCG+TGG	-	chr5_2:64464231-64464250	Msa0781830:intron	45.0%
	GTTCGCACCATTTAAACCGT+GGG	-	chr5_2:64464232-64464251	Msa0781830:intron	45.0%
!	TGTTGTGTTCTGTTTGGAGC+AGG	-	chr5_2:64464421-64464440	Msa0781830:CDS	45.0%
	GAGCAGGATAAAGTGCAGAA+GGG	-	chr5_2:64464437-64464456	Msa0781830:CDS	45.0%
	AGGATAAAGTGCAGAAGGGA+AGG	-	chr5_2:64464441-64464460	Msa0781830:CDS	45.0%
	ATAAAGTGCAGAAGGGAAGG+TGG	-	chr5_2:64464444-64464463	Msa0781830:CDS	45.0%
	CAGATCCGGTGACTGTAAAT+CGG	+	chr5_2:64464473-64464492	None:intergenic	45.0%
	CATTGTTGCGGTGAAGGTTA+AGG	-	chr5_2:64464539-64464558	Msa0781830:CDS	45.0%
	AAGGTTAAGGGCTCAAGAAC+AGG	-	chr5_2:64464552-64464571	Msa0781830:CDS	45.0%
	TTAAGGGCTCAAGAACAGGT+TGG	-	chr5_2:64464556-64464575	Msa0781830:CDS	45.0%
	GCTTTCAATGGGTCGGAATT+GGG	-	chr5_2:64464578-64464597	Msa0781830:CDS	45.0%
	CTTTCAATGGGTCGGAATTG+GGG	-	chr5_2:64464579-64464598	Msa0781830:CDS	45.0%
	ACAATGTTGCTCCCAAAGAC+TGG	-	chr5_2:64464688-64464707	Msa0781830:CDS	45.0%
	AATGGGAAAGGGAAAGAGAC+TGG	+	chr5_2:64464788-64464807	None:intergenic	45.0%
	TGTTGTTGCAATGGGTGGGT+GGG	+	chr5_2:64461671-64461690	None:intergenic	50.0%
!	TTGTTGTTGCAATGGGTGGG+TGG	+	chr5_2:64461672-64461691	None:intergenic	50.0%
!	AGAAGGTGGTGGTTGAGAAG+AGG	+	chr5_2:64461888-64461907	None:intergenic	50.0%
	GGGGAAGATTGTTGAGAAGG+TGG	+	chr5_2:64461902-64461921	None:intergenic	50.0%
	CTTCCCCTTCTTTCACCGAA+TGG	-	chr5_2:64461915-64461934	Msa0781830:three_prime_UTR	50.0%
!	TCGCCATTCGGTGAAAGAAG+GGG	+	chr5_2:64461921-64461940	None:intergenic	50.0%
!	GATCGCCATTCGGTGAAAGA+AGG	+	chr5_2:64461923-64461942	None:intergenic	50.0%
	ACTGTGTCACGTGGATCTAC+CGG	+	chr5_2:64461968-64461987	None:intergenic	50.0%
	GTAGATCCACGTGACACAGT+CGG	-	chr5_2:64461968-64461987	Msa0781830:CDS	50.0%
	ATCAAAAGCCGGATACGGCA+TGG	-	chr5_2:64462015-64462034	Msa0781830:CDS	50.0%
	AACAGTTGCCATGCCGTATC+CGG	+	chr5_2:64462026-64462045	None:intergenic	50.0%
!	GTGGCGCGTGCTTTGAATTA+CGG	-	chr5_2:64462083-64462102	Msa0781830:CDS	50.0%
	ACATGCCCCTTCAGTATCGT+AGG	-	chr5_2:64462284-64462303	Msa0781830:intron	50.0%
	ATTATCAGCCTCGACGTGTG+GGG	+	chr5_2:64462481-64462500	None:intergenic	50.0%
	CACAACACTCTGACGCATGT+TGG	-	chr5_2:64462542-64462561	Msa0781830:intron	50.0%
	TGAGCTTCAGCTCGATCCAA+CGG	-	chr5_2:64463074-64463093	Msa0781830:intron	50.0%
	CCCAATCAAATCTCGTCCGT+TGG	+	chr5_2:64463093-64463112	None:intergenic	50.0%
	CGGACGAGATTTGATTGGGT+TGG	-	chr5_2:64463094-64463113	Msa0781830:intron	50.0%
!	AGAAAGGTGTGCATCCTTGG+AGG	+	chr5_2:64463349-64463368	None:intergenic	50.0%
	TGTTTGGGAGTTCAGAGAGG+AGG	-	chr5_2:64463494-64463513	Msa0781830:intron	50.0%
	GTTTGGGAGTTCAGAGAGGA+GGG	-	chr5_2:64463495-64463514	Msa0781830:intron	50.0%
	TTTGGGAGTTCAGAGAGGAG+GGG	-	chr5_2:64463496-64463515	Msa0781830:intron	50.0%
!!	AGGGGAGGGCTTTGGAAAAA+AGG	+	chr5_2:64463815-64463834	None:intergenic	50.0%
	TTTGCGAGTTTGGAGGGGAA+GGG	+	chr5_2:64463843-64463862	None:intergenic	50.0%
	AAACCGTGTACGAAACAGGC+CGG	-	chr5_2:64464164-64464183	Msa0781830:intron	50.0%
	GGAGCAGGATAAAGTGCAGA+AGG	-	chr5_2:64464436-64464455	Msa0781830:CDS	50.0%
	GGAGTCCGATTTACAGTCAC+CGG	-	chr5_2:64464465-64464484	Msa0781830:CDS	50.0%
	CGATTTACAGTCACCGGATC+TGG	-	chr5_2:64464471-64464490	Msa0781830:CDS	50.0%
!	GTGCTGATCAGCAATGTTGC+TGG	-	chr5_2:64464507-64464526	Msa0781830:CDS	50.0%
	ATCAGCAATGTTGCTGGCCA+TGG	-	chr5_2:64464513-64464532	Msa0781830:CDS	50.0%
	TGGCCATGGAGACATTGTTG+CGG	-	chr5_2:64464527-64464546	Msa0781830:CDS	50.0%
	TCACCGCAACAATGTCTCCA+TGG	+	chr5_2:64464533-64464552	None:intergenic	50.0%
	TGGAGACATTGTTGCGGTGA+AGG	-	chr5_2:64464533-64464552	Msa0781830:CDS	50.0%
!!	CAGGTTGGCTTTCAATGGGT+CGG	-	chr5_2:64464571-64464590	Msa0781830:CDS	50.0%
	GGCTTTCAATGGGTCGGAAT+TGG	-	chr5_2:64464577-64464596	Msa0781830:CDS	50.0%
!	GCTCCCAAAGACTGGAGTTT+TGG	-	chr5_2:64464696-64464715	Msa0781830:CDS	50.0%
!	AGAGACTGGTCCCTTGAAAC+AGG	+	chr5_2:64464774-64464793	None:intergenic	50.0%
	TGTGTCACGTGGATCTACCG+GGG	+	chr5_2:64461966-64461985	None:intergenic	55.0%
	CTGTGTCACGTGGATCTACC+GGG	+	chr5_2:64461967-64461986	None:intergenic	55.0%
	GGATACGGCATGGCAACTGT+TGG	-	chr5_2:64462025-64462044	Msa0781830:CDS	55.0%
	GGGTTTACGGTTGAAGGTGG+TGG	-	chr5_2:64462184-64462203	Msa0781830:CDS	55.0%
	TAGACAGTGGCAGAGGATCC+TGG	+	chr5_2:64463032-64463051	None:intergenic	55.0%
!!	GGGTTGGCTGCAAAGGTTTC+TGG	-	chr5_2:64463110-64463129	Msa0781830:intron	55.0%
	GGAGGAGCAGTCAAGTGGTA+TGG	+	chr5_2:64463331-64463350	None:intergenic	55.0%
	ACCACTTGACTGCTCCTCCA+AGG	-	chr5_2:64463332-64463351	Msa0781830:intron	55.0%
	TCCTTGGAGGAGCAGTCAAG+TGG	+	chr5_2:64463336-64463355	None:intergenic	55.0%
	GAGTTCAGAGAGGAGGGGAA+GGG	-	chr5_2:64463501-64463520	Msa0781830:intron	55.0%
!!	AAGGGAGGGCTTTGAGGGAT+GGG	-	chr5_2:64463519-64463538	Msa0781830:intron	55.0%
!!	TAGTAGGCTTTGGGAGCGGA+GGG	+	chr5_2:64463773-64463792	None:intergenic	55.0%
!!	ATAGTAGGCTTTGGGAGCGG+AGG	+	chr5_2:64463774-64463793	None:intergenic	55.0%
	GTTTGCGAGTTTGGAGGGGA+AGG	+	chr5_2:64463844-64463863	None:intergenic	55.0%
	TAGGGCTGAGTGTGCATTGC+AGG	-	chr5_2:64464126-64464145	Msa0781830:intron	55.0%
	AACCGTGTACGAAACAGGCC+GGG	-	chr5_2:64464165-64464184	Msa0781830:intron	55.0%
	GTCGGAATTGGGGCCAAAAC+TGG	-	chr5_2:64464589-64464608	Msa0781830:CDS	55.0%
	ATCTACCGGGGCGTAGTAGG+TGG	+	chr5_2:64461954-64461973	None:intergenic	60.0%
	TGGATCTACCGGGGCGTAGT+AGG	+	chr5_2:64461957-64461976	None:intergenic	60.0%
	GATCCACGTGACACAGTCGG+CGG	-	chr5_2:64461971-64461990	Msa0781830:CDS	60.0%
	GACACTAACCCCACACGTCG+AGG	-	chr5_2:64462470-64462489	Msa0781830:intron	60.0%
	GGAGTTCAGAGAGGAGGGGA+AGG	-	chr5_2:64463500-64463519	Msa0781830:intron	60.0%
	TTCAGAGAGGAGGGGAAGGG+AGG	-	chr5_2:64463504-64463523	Msa0781830:intron	60.0%
	TCAGAGAGGAGGGGAAGGGA+GGG	-	chr5_2:64463505-64463524	Msa0781830:intron	60.0%
!	AGGGGAAGGGAGGGCTTTGA+GGG	-	chr5_2:64463514-64463533	Msa0781830:intron	60.0%
!!	GAAGGGAGGGCTTTGAGGGA+TGG	-	chr5_2:64463518-64463537	Msa0781830:intron	60.0%
	TTTGGGAGCGGAGGGCTTTG+GGG	+	chr5_2:64463765-64463784	None:intergenic	60.0%
!	CTTTGGGAGCGGAGGGCTTT+GGG	+	chr5_2:64463766-64463785	None:intergenic	60.0%
	GAGTTTGGAGGGGAAGGGAG+GGG	+	chr5_2:64463838-64463857	None:intergenic	60.0%
	CGAGTTTGGAGGGGAAGGGA+GGG	+	chr5_2:64463839-64463858	None:intergenic	60.0%
	AGCCCGGCCTGTTTCGTACA+CGG	+	chr5_2:64464170-64464189	None:intergenic	60.0%
	GCAATGGGTGGGTGGGACAC+TGG	+	chr5_2:64461664-64461683	None:intergenic	65.0%
	GGCACGTGCGGATCGCCATT+CGG	+	chr5_2:64461933-64461952	None:intergenic	65.0%
	GGCGTAGTAGGTGGCACGTG+CGG	+	chr5_2:64461945-64461964	None:intergenic	65.0%
	ACGTGCCACCTACTACGCCC+CGG	-	chr5_2:64461946-64461965	Msa0781830:exon	65.0%
	GAGGGGAAGGGAGGGCTTTG+AGG	-	chr5_2:64463513-64463532	Msa0781830:intron	65.0%
!	TTGGGAGCGGAGGGCTTTGG+GGG	+	chr5_2:64463764-64463783	None:intergenic	65.0%
!!	GCTTTGGGAGCGGAGGGCTT+TGG	+	chr5_2:64463767-64463786	None:intergenic	65.0%
	TTGGAGGGGAAGGGAGGGGA+GGG	+	chr5_2:64463834-64463853	None:intergenic	65.0%
	TTTGGAGGGGAAGGGAGGGG+AGG	+	chr5_2:64463835-64463854	None:intergenic	65.0%
	GCGAGTTTGGAGGGGAAGGG+AGG	+	chr5_2:64463840-64463859	None:intergenic	65.0%
	TGGAGGGGAAGGGAGGGGAG+GGG	+	chr5_2:64463833-64463852	None:intergenic	70.0%
!	GCGCCGCCGACTGTGTCACG+TGG	+	chr5_2:64461977-64461996	None:intergenic	75.0%
	GACACAGTCGGCGGCGCGTG+TGG	-	chr5_2:64461980-64461999	Msa0781830:CDS	75.0%
	GTCGGCGGCGCGTGTGGCTA+CGG	-	chr5_2:64461986-64462005	Msa0781830:CDS	75.0%
	GGGAGGGGAGGGGAGGGCTT+TGG	+	chr5_2:64463823-64463842	None:intergenic	75.0%
	GGGGAAGGGAGGGGAGGGGA+GGG	+	chr5_2:64463829-64463848	None:intergenic	75.0%
	AGGGGAAGGGAGGGGAGGGG+AGG	+	chr5_2:64463830-64463849	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	64461646	64465067	64461646	ID=Msa0781830;Name=Msa0781830
chr5_2	mRNA	64461646	64465067	64461646	ID=Msa0781830-mRNA-1;Parent=Msa0781830;Name=Msa0781830-mRNA-1;_AED=0.05;_eAED=0.05;_QI=169\|1\|1\|1\|1\|1\|2\|315\|266
chr5_2	exon	64461646	64462270	64461646	ID=Msa0781830-mRNA-1:exon:19245;Parent=Msa0781830-mRNA-1
chr5_2	exon	64464408	64465067	64464408	ID=Msa0781830-mRNA-1:exon:19244;Parent=Msa0781830-mRNA-1
chr5_2	five_prime_UTR	64464899	64465067	64464899	ID=Msa0781830-mRNA-1:five_prime_utr;Parent=Msa0781830-mRNA-1
chr5_2	CDS	64464408	64464898	64464408	ID=Msa0781830-mRNA-1:cds;Parent=Msa0781830-mRNA-1
chr5_2	CDS	64461961	64462270	64461961	ID=Msa0781830-mRNA-1:cds;Parent=Msa0781830-mRNA-1
chr5_2	three_prime_UTR	64461646	64461960	64461646	ID=Msa0781830-mRNA-1:three_prime_utr;Parent=Msa0781830-mRNA-1

Gene Sequence

>Msa0781830

ATGTTACTTACAACGTTCTTCTTTCTCTTTACACTCTCATCAACAACCGTCACCTCACACTACTCTTCTTCCTCTTCTCAACCACCACCTTCTCAACAATCTTCCCCTTCTTTCACCGAATGGCGATCCGCACGTGCCACCTACTACGCCCCGGTAGATCCACGTGACACAGTCGGCGGCGCGTGTGGCTACGGAGACTTATCAAAAGCCGGATACGGCATGGCAACTGTTGGTCTTAGTGAAGCTTTGTTTGAACGAGGTCAGATCTGTGGCGCGTGCTTTGAATTACGGTGTGTGGATGATTTAAAGTGGTGTATTCCTGGAACTTCGATTATTGTTACTGCGACGAATTTCTGTGCGCCGAATTATGGGTTTACGGTTGAAGGTGGTGGACATTGTAATCCTCCTAATAAGCACTTTGTTCTTCCTATTGATGCTTTTGAGAAGATCGCTATTTGGAAAGCTGGTAACATGCCCCTTCAGTATCGTAGGATAAAGTGCAGAAGGGAAGGTGGAGTCCGATTTACAGTCACCGGATCTGGTATCTTCATTTCAGTGCTGATCAGCAATGTTGCTGGCCATGGAGACATTGTTGCGGTGAAGGTTAAGGGCTCAAGAACAGGTTGGCTTTCAATGGGTCGGAATTGGGGCCAAAACTGGCATATAAACGCGTTACTACAGAATCAACCTCTTTCATTTGAGGTTACGAGTAGTGATGGGAAAACAGTCACGGCTTACAATGTTGCTCCCAAAGACTGGAGTTTTGGACAAACCTTTGAAGGCAAGCAATTTGATTATTAA

Protein sequence

>Msa0781830

MLLTTFFFLFTLSSTTVTSHYSSSSSQPPPSQQSSPSFTEWRSARATYYAPVDPRDTVGGACGYGDLSKAGYGMATVGLSEALFERGQICGACFELRCVDDLKWCIPGTSIIVTATNFCAPNYGFTVEGGGHCNPPNKHFVLPIDAFEKIAIWKAGNMPLQYRRIKCRREGGVRFTVTGSGIFISVLISNVAGHGDIVAVKVKGSRTGWLSMGRNWGQNWHINALLQNQPLSFEVTSSDGKTVTAYNVAPKDWSFGQTFEGKQFDY*