AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0795840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0795840 MtrunA17_Chr5g0398841 77.632 152 9 2 1 128 6 156 1.59e-69 206
Msa0795840 MtrunA17_Chr5g0398821 52.000 150 43 6 5 128 9 155 4.68e-40 131
Msa0795840 MtrunA17_Chr5g0398831 44.231 156 49 8 5 127 10 160 1.61e-27 99.4
Msa0795840 MtrunA17_Chr4g0056621 32.468 154 72 7 1 128 1 148 1.39e-17 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTCAAATTTCAGTTCC+TGG 0.242271 5_3:-7874482 Msa0795840:CDS
ACTTCATATTGTTGTTCATT+TGG 0.271537 5_3:-7872354 Msa0795840:CDS
CATTTGCTGCAATCCAATTA+AGG 0.297545 5_3:-7874537 Msa0795840:CDS
GCATGTATGTTGTTTCATTA+AGG 0.310653 5_3:-7872331 Msa0795840:exon
CTTCATATTGTTGTTCATTT+GGG 0.320896 5_3:-7872353 Msa0795840:CDS
CCTGGCCTACTCGAAAGTTT+TGG 0.354767 5_3:-7874464 Msa0795840:intron
CCTGGAGGTTGCAGCAATGA+TGG 0.402054 5_3:+7873993 None:intergenic
GACAGAAACATGTATAATCA+TGG 0.403279 5_3:+7874498 None:intergenic
TTATGAGGATGATGGTGGTT+TGG 0.421436 5_3:-7874682 Msa0795840:CDS
GGAAGTGATGACGGAGGAGC+GGG 0.427720 5_3:-7872400 Msa0795840:CDS
AAAATTGCAGCTTCTTCTCC+AGG 0.461247 5_3:-7874023 Msa0795840:intron
ATTAAGGAGAATTTCTTGCT+TGG 0.461758 5_3:-7872315 Msa0795840:exon
ATTGGATTGCAGCAAATGTC+TGG 0.495953 5_3:+7874542 None:intergenic
CCATCATTGCTGCAACCTCC+AGG 0.500501 5_3:-7873993 Msa0795840:intron
TGGAAGTGATGACGGAGGAG+CGG 0.513346 5_3:-7872401 Msa0795840:CDS
ATTGCAGAATGTCTATTATG+AGG 0.514995 5_3:-7874697 None:intergenic
GCGGGGATGATCACCTTCAC+TGG 0.521361 5_3:-7872382 Msa0795840:CDS
CAAATGCTTCCACCTTGTGC+TGG 0.524026 5_3:-7874611 Msa0795840:CDS
CCAAAACTTTCGAGTAGGCC+AGG 0.539426 5_3:+7874464 None:intergenic
ACAGAAACATGTATAATCAT+GGG 0.545168 5_3:+7874499 None:intergenic
TAACACCAAAACTTTCGAGT+AGG 0.584719 5_3:+7874459 None:intergenic
GCAACACGTGGAAGTGATGA+CGG 0.593939 5_3:-7872409 Msa0795840:CDS
ATTTCTTGATCTTAACGAAG+AGG 0.601751 5_3:+7872174 None:intergenic
AATGTCTATTATGAGGATGA+TGG 0.602352 5_3:-7874690 Msa0795840:CDS
CTGGTGGATGAGTGGAATTG+AGG 0.608601 5_3:+7874561 None:intergenic
GCAAATGTCTGGTGGATGAG+TGG 0.618915 5_3:+7874553 None:intergenic
TATGAAGTAGTGTCCAGTGA+AGG 0.634695 5_3:+7872369 None:intergenic
GTCTATTATGAGGATGATGG+TGG 0.635774 5_3:-7874687 Msa0795840:CDS
TTGGTTTAGATAGTTGTCCG+TGG 0.641346 5_3:+7872140 None:intergenic
AGCAATGATGGAGAAAGACC+TGG 0.643773 5_3:+7874005 None:intergenic
TACGAATTTGCAGCAACACG+TGG 0.655516 5_3:-7872421 Msa0795840:intron
AACATCTTATCACCAGCACA+AGG 0.675106 5_3:+7874599 None:intergenic
GAAGTGATGACGGAGGAGCG+GGG 0.687350 5_3:-7872399 Msa0795840:CDS
GAATTGAGGTAGTCTGTGCA+TGG 0.688761 5_3:+7874575 None:intergenic
ATCTTATCACCAGCACAAGG+TGG 0.690594 5_3:+7874602 None:intergenic
GGATTGCAGCAAATGTCTGG+TGG 0.693607 5_3:+7874545 None:intergenic
ACACGTGGAAGTGATGACGG+AGG 0.768255 5_3:-7872406 Msa0795840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTATTTTTAAACTT+AGG + chr5_3:7873120-7873139 None:intergenic 10.0%
!! ATAATTCCATTAAAAAAATA+AGG - chr5_3:7873250-7873269 Msa0795840:intron 10.0%
!!! TTTTTTCCTTATTTTTTTAA+TGG + chr5_3:7873259-7873278 None:intergenic 10.0%
!!! TTATTTCTATTTGAAATATA+GGG + chr5_3:7873353-7873372 None:intergenic 10.0%
!!! ATTATTTCTATTTGAAATAT+AGG + chr5_3:7873354-7873373 None:intergenic 10.0%
!! AAAAAAAATTCTCATAAATA+TGG - chr5_3:7873629-7873648 Msa0795840:intron 10.0%
!!! CTTTTATTAAAAATTCATTT+TGG + chr5_3:7873920-7873939 None:intergenic 10.0%
!!! GAATTTTTAATAAAAGATAA+AGG - chr5_3:7873924-7873943 Msa0795840:intron 10.0%
!! TTTAAACATTATAAAAACAA+TGG + chr5_3:7874116-7874135 None:intergenic 10.0%
!!! ATATATCATCCAATTATTTT+CGG - chr5_3:7872541-7872560 Msa0795840:intron 15.0%
!!! TTTTAAAAGAATTTTGTCTA+CGG - chr5_3:7872605-7872624 Msa0795840:intron 15.0%
!!! TTTTTTAGTAGAAAATAATC+AGG + chr5_3:7872762-7872781 None:intergenic 15.0%
!!! GTAGTTTTCTTTAAATAAAT+AGG - chr5_3:7873181-7873200 Msa0795840:intron 15.0%
!!! TTTTTTTAAAATAGACGAAA+TGG + chr5_3:7873610-7873629 None:intergenic 15.0%
!!! AAATAACATACTTTTTTCTT+CGG - chr5_3:7873681-7873700 Msa0795840:intron 15.0%
!!! AATCCTTTATCTTTTTAAAA+AGG - chr5_3:7873759-7873778 Msa0795840:intron 15.0%
!!! AACTTTTTTCTAAATCATTT+TGG + chr5_3:7873891-7873910 None:intergenic 15.0%
!!! ACATCTCAAATTTTTAAAAT+TGG + chr5_3:7874557-7874576 None:intergenic 15.0%
!! ACACATATATATGAGTAATA+TGG + chr5_3:7872473-7872492 None:intergenic 20.0%
!!! TGTGTGAAATTTTAAAAACA+TGG - chr5_3:7872488-7872507 Msa0795840:intron 20.0%
!! ATTATTGTACCGAAAATAAT+TGG + chr5_3:7872553-7872572 None:intergenic 20.0%
!!! AATAAATTACCTTTACGTTT+TGG - chr5_3:7872791-7872810 Msa0795840:intron 20.0%
!! TAAGATAATAGTTAAAAGAC+AGG - chr5_3:7873285-7873304 Msa0795840:intron 20.0%
!!! TGTATCTATAATCTTTTTTG+AGG - chr5_3:7873388-7873407 Msa0795840:intron 20.0%
!!! GTATCTATAATCTTTTTTGA+GGG - chr5_3:7873389-7873408 Msa0795840:intron 20.0%
!!! TCACCTTTTTAAAAAGATAA+AGG + chr5_3:7873765-7873784 None:intergenic 20.0%
!!! ATTTTAGTCCTTATTTTCAT+TGG - chr5_3:7873786-7873805 Msa0795840:intron 20.0%
!!! ATCACTCTTTAAAAAGATAA+AGG - chr5_3:7873835-7873854 Msa0795840:intron 20.0%
!! TATCATATGCATAAAAAAAG+TGG - chr5_3:7874020-7874039 Msa0795840:intron 20.0%
!! AATGATGTAAAATTATTAGC+CGG - chr5_3:7874273-7874292 Msa0795840:intron 20.0%
!!! ATTTTTTTTAAAATGAGTCC+CGG + chr5_3:7874295-7874314 None:intergenic 20.0%
!!! CAAGCTAATTTTATTTGTAT+TGG - chr5_3:7874624-7874643 Msa0795840:CDS 20.0%
! GCTTCAAAAATTTCCTTAAT+TGG + chr5_3:7872316-7872335 None:intergenic 25.0%
! ACAGAAACATGTATAATCAT+GGG + chr5_3:7872341-7872360 None:intergenic 25.0%
!!! GTTTTTTGAACAAGCAAAAA+TGG + chr5_3:7872667-7872686 None:intergenic 25.0%
!!! TTTCTCTCTGTCTTTATTTT+TGG - chr5_3:7872882-7872901 Msa0795840:intron 25.0%
!!! TTCTCTCTGTCTTTATTTTT+GGG - chr5_3:7872883-7872902 Msa0795840:intron 25.0%
! CTTTGTAAGTAAAAGTTGTT+TGG + chr5_3:7873143-7873162 None:intergenic 25.0%
!! AATCAAAGAGTACAAAACAA+TGG - chr5_3:7873420-7873439 Msa0795840:intron 25.0%
! GCTTGGCTTAAATATATATT+TGG - chr5_3:7873707-7873726 Msa0795840:intron 25.0%
! TACCAAAACCAATGAAAATA+AGG + chr5_3:7873797-7873816 None:intergenic 25.0%
! ATGATGTAAAATTATTAGCC+GGG - chr5_3:7874274-7874293 Msa0795840:intron 25.0%
!!! GGGACTCATTTTAAAAAAAA+TGG - chr5_3:7874294-7874313 Msa0795840:intron 25.0%
!!! TCTCTTAGTAAAAGTGTTAT+TGG - chr5_3:7874343-7874362 Msa0795840:intron 25.0%
!! ACTTCATATTGTTGTTCATT+TGG - chr5_3:7874483-7874502 Msa0795840:CDS 25.0%
!! CTTCATATTGTTGTTCATTT+GGG - chr5_3:7874484-7874503 Msa0795840:CDS 25.0%
!!! TTTTTTCATTATTGTGTGTG+TGG - chr5_3:7874601-7874620 Msa0795840:CDS 25.0%
!!! AGAAATTGACATTTTTTCCA+CGG - chr5_3:7874680-7874699 Msa0795840:CDS 25.0%
GACAGAAACATGTATAATCA+TGG + chr5_3:7872342-7872361 None:intergenic 30.0%
!!! TTTTGGTGTTAAACTAGCTT+TGG - chr5_3:7872390-7872409 Msa0795840:CDS 30.0%
GTATTATGTACCTTGAAGTT+TGG + chr5_3:7872645-7872664 None:intergenic 30.0%
! TGCAATTTTCCAAAACGTAA+AGG + chr5_3:7872803-7872822 None:intergenic 30.0%
AATGAGTCCTAAGAATTGTT+TGG + chr5_3:7872913-7872932 None:intergenic 30.0%
AAACAATGGATACATTGACA+CGG - chr5_3:7873434-7873453 Msa0795840:intron 30.0%
!!! TCCACAAAGGTATTTTTCTA+GGG - chr5_3:7873657-7873676 Msa0795840:intron 30.0%
!!! GTCCTTATTTTCATTGGTTT+TGG - chr5_3:7873792-7873811 Msa0795840:intron 30.0%
TAAAAAAAATGGTGTGCATG+TGG - chr5_3:7874305-7874324 Msa0795840:intron 30.0%
AAAAAAAATGGTGTGCATGT+GGG - chr5_3:7874306-7874325 Msa0795840:intron 30.0%
GTAAAAACTCAGGATCATAT+TGG + chr5_3:7874399-7874418 None:intergenic 30.0%
!! GCATGTATGTTGTTTCATTA+AGG - chr5_3:7874506-7874525 Msa0795840:CDS 30.0%
ATTAAGGAGAATTTCTTGCT+TGG - chr5_3:7874522-7874541 Msa0795840:CDS 30.0%
ATTTCTTGATCTTAACGAAG+AGG + chr5_3:7874666-7874685 None:intergenic 30.0%
! CATTTGCTGCAATCCAATTA+AGG - chr5_3:7872300-7872319 Msa0795840:three_prime_UTR 35.0%
TTCTGTCAAATTTCAGTTCC+TGG - chr5_3:7872355-7872374 Msa0795840:CDS 35.0%
! TAACACCAAAACTTTCGAGT+AGG + chr5_3:7872381-7872400 None:intergenic 35.0%
GTCAAAGTTGTTAACTCATG+TGG - chr5_3:7872415-7872434 Msa0795840:intron 35.0%
CACAATAGCATATGAAGCTT+TGG - chr5_3:7872512-7872531 Msa0795840:intron 35.0%
! TTGAAGTTTGGTGAGATACT+TGG + chr5_3:7872633-7872652 None:intergenic 35.0%
AAGTATCTCACCAAACTTCA+AGG - chr5_3:7872632-7872651 Msa0795840:intron 35.0%
GGACATCAATGCTTTAGTTA+AGG + chr5_3:7872741-7872760 None:intergenic 35.0%
! AAATTGAGAGGCTTTTTACC+TGG + chr5_3:7872865-7872884 None:intergenic 35.0%
AAAGACAGAGAGAAATTGAG+AGG + chr5_3:7872877-7872896 None:intergenic 35.0%
GGGATTACCAAACAATTCTT+AGG - chr5_3:7872903-7872922 Msa0795840:intron 35.0%
TTACTTACAAAGTGACACAC+CGG - chr5_3:7873151-7873170 Msa0795840:intron 35.0%
GGCAAAATGCTGAAATTGTT+AGG - chr5_3:7873522-7873541 Msa0795840:intron 35.0%
AAATGCTGAAATTGTTAGGC+CGG - chr5_3:7873526-7873545 Msa0795840:intron 35.0%
CATTATAAACTCACAAGTGG+AGG + chr5_3:7873581-7873600 None:intergenic 35.0%
CTCCACTTGTGAGTTTATAA+TGG - chr5_3:7873579-7873598 Msa0795840:intron 35.0%
AGCCATTATAAACTCACAAG+TGG + chr5_3:7873584-7873603 None:intergenic 35.0%
CTAGAAAAATACCTTTGTGG+AGG + chr5_3:7873658-7873677 None:intergenic 35.0%
!!! CTCCACAAAGGTATTTTTCT+AGG - chr5_3:7873656-7873675 Msa0795840:intron 35.0%
TCCCTAGAAAAATACCTTTG+TGG + chr5_3:7873661-7873680 None:intergenic 35.0%
!! TGGAAAGTATTGCTAGTGTT+AGG - chr5_3:7874040-7874059 Msa0795840:intron 35.0%
CTGCAAATTCGTAAAAACTC+AGG + chr5_3:7874409-7874428 None:intergenic 35.0%
! GTCTATTATGAGGATGATGG+TGG - chr5_3:7872150-7872169 Msa0795840:three_prime_UTR 40.0%
!! TTATGAGGATGATGGTGGTT+TGG - chr5_3:7872155-7872174 Msa0795840:three_prime_UTR 40.0%
AACATCTTATCACCAGCACA+AGG + chr5_3:7872241-7872260 None:intergenic 40.0%
ATTGGATTGCAGCAAATGTC+TGG + chr5_3:7872298-7872317 None:intergenic 40.0%
TAATATGGTACCTTTGCAGG+AGG + chr5_3:7872458-7872477 None:intergenic 40.0%
GAGTAATATGGTACCTTTGC+AGG + chr5_3:7872461-7872480 None:intergenic 40.0%
AAAATTGCAGCTTCTTCTCC+AGG - chr5_3:7872814-7872833 Msa0795840:intron 40.0%
AAAGAAAACTACTCAGCCAC+CGG + chr5_3:7873173-7873192 None:intergenic 40.0%
GAAAAATACCTTTGTGGAGG+CGG + chr5_3:7873655-7873674 None:intergenic 40.0%
!!! ACTTTTTTCTTCGGTTCGCT+TGG - chr5_3:7873690-7873709 Msa0795840:intron 40.0%
TATGAAGTAGTGTCCAGTGA+AGG + chr5_3:7874471-7874490 None:intergenic 40.0%
ATCTTATCACCAGCACAAGG+TGG + chr5_3:7872238-7872257 None:intergenic 45.0%
GAATTGAGGTAGTCTGTGCA+TGG + chr5_3:7872265-7872284 None:intergenic 45.0%
! AGCAATGATGGAGAAAGACC+TGG + chr5_3:7872835-7872854 None:intergenic 45.0%
! TTGAGAGGCTTTTTACCTGG+AGG + chr5_3:7872862-7872881 None:intergenic 45.0%
! AGCATTTTGCCAGTTGAGCT+AGG + chr5_3:7873513-7873532 None:intergenic 45.0%
TACGAATTTGCAGCAACACG+TGG - chr5_3:7874416-7874435 Msa0795840:intron 45.0%
CAAATGCTTCCACCTTGTGC+TGG - chr5_3:7872226-7872245 Msa0795840:three_prime_UTR 50.0%
! CTGGTGGATGAGTGGAATTG+AGG + chr5_3:7872279-7872298 None:intergenic 50.0%
! GCAAATGTCTGGTGGATGAG+TGG + chr5_3:7872287-7872306 None:intergenic 50.0%
GGATTGCAGCAAATGTCTGG+TGG + chr5_3:7872295-7872314 None:intergenic 50.0%
! CCAAAACTTTCGAGTAGGCC+AGG + chr5_3:7872376-7872395 None:intergenic 50.0%
! CCTGGCCTACTCGAAAGTTT+TGG - chr5_3:7872373-7872392 Msa0795840:CDS 50.0%
ATGGTACCTTTGCAGGAGGT+GGG + chr5_3:7872454-7872473 None:intergenic 50.0%
TATGGTACCTTTGCAGGAGG+TGG + chr5_3:7872455-7872474 None:intergenic 50.0%
CTTACAAAGTGACACACCGG+TGG - chr5_3:7873154-7873173 Msa0795840:intron 50.0%
TCCAGAAGTCCTAGCTCAAC+TGG - chr5_3:7873501-7873520 Msa0795840:intron 50.0%
ACTCACAAGTGGAGGAACCA+GGG + chr5_3:7873573-7873592 None:intergenic 50.0%
AACTCACAAGTGGAGGAACC+AGG + chr5_3:7873574-7873593 None:intergenic 50.0%
AAATATGGCCGCCTCCACAA+AGG - chr5_3:7873644-7873663 Msa0795840:intron 50.0%
GCAACACGTGGAAGTGATGA+CGG - chr5_3:7874428-7874447 Msa0795840:intron 50.0%
TTCGATCCCACCTCCTGCAA+AGG - chr5_3:7872445-7872464 Msa0795840:intron 55.0%
CCTGGAGGTTGCAGCAATGA+TGG + chr5_3:7872847-7872866 None:intergenic 55.0%
CCATCATTGCTGCAACCTCC+AGG - chr5_3:7872844-7872863 Msa0795840:intron 55.0%
GCCAGTTGAGCTAGGACTTC+TGG + chr5_3:7873505-7873524 None:intergenic 55.0%
!! ACACGTGGAAGTGATGACGG+AGG - chr5_3:7874431-7874450 Msa0795840:intron 55.0%
!! TGGAAGTGATGACGGAGGAG+CGG - chr5_3:7874436-7874455 Msa0795840:intron 55.0%
GTTAGGCCGGATGCCATGAC+CGG - chr5_3:7873539-7873558 Msa0795840:intron 60.0%
TTAGGCCGGATGCCATGACC+GGG - chr5_3:7873540-7873559 Msa0795840:intron 60.0%
AGGAACCAGGGTTCGAACCC+CGG + chr5_3:7873561-7873580 None:intergenic 60.0%
!! GGAAGTGATGACGGAGGAGC+GGG - chr5_3:7874437-7874456 Msa0795840:intron 60.0%
!! GAAGTGATGACGGAGGAGCG+GGG - chr5_3:7874438-7874457 Msa0795840:intron 60.0%
GCGGGGATGATCACCTTCAC+TGG - chr5_3:7874455-7874474 Msa0795840:intron 60.0%
TAGGCCGGATGCCATGACCG+GGG - chr5_3:7873541-7873560 Msa0795840:intron 65.0%
CGAACCCCGGTCATGGCATC+CGG + chr5_3:7873548-7873567 None:intergenic 65.0%
CAGGGTTCGAACCCCGGTCA+TGG + chr5_3:7873555-7873574 None:intergenic 65.0%
CATGACCGGGGTTCGAACCC+TGG - chr5_3:7873553-7873572 Msa0795840:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 7872148 7874711 7872148 ID=Msa0795840;Name=Msa0795840
chr5_3 mRNA 7872148 7874711 7872148 ID=Msa0795840-mRNA-1;Parent=Msa0795840;Name=Msa0795840-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.5|0.66|1|0|0.33|3|185|128
chr5_3 exon 7872148 7872430 7872148 ID=Msa0795840-mRNA-1:exon:3257;Parent=Msa0795840-mRNA-1
chr5_3 exon 7873994 7874035 7873994 ID=Msa0795840-mRNA-1:exon:3256;Parent=Msa0795840-mRNA-1
chr5_3 exon 7874465 7874711 7874465 ID=Msa0795840-mRNA-1:exon:3255;Parent=Msa0795840-mRNA-1
chr5_3 CDS 7874465 7874711 7874465 ID=Msa0795840-mRNA-1:cds;Parent=Msa0795840-mRNA-1
chr5_3 CDS 7873994 7874035 7873994 ID=Msa0795840-mRNA-1:cds;Parent=Msa0795840-mRNA-1
chr5_3 CDS 7872333 7872430 7872333 ID=Msa0795840-mRNA-1:cds;Parent=Msa0795840-mRNA-1
chr5_3 three_prime_UTR 7872148 7872332 7872148 ID=Msa0795840-mRNA-1:three_prime_utr;Parent=Msa0795840-mRNA-1
Gene Sequence

>Msa0795840

ATGTCTATTATGAGGATGATGGTGGTTTGGTTGATTATGTTCGTAATTAACATAAATGTAATTAGCTTAGTAGATGCACAAATGCTTCCACCTTGTGCTGGTGATAAGATGTTGCCATGCACAGACTACCTCAATTCCACTCATCCACCAGACATTTGCTGCAATCCAATTAAGGAAATTTTTGAAGCAACCCATGATTATACATGTTTCTGTCAAATTTCAGTTCCTGGCCTACTCGAAAGTTTTGCTTCTTCTCCAGGTCTTTCTCCATCATTGCTGCAACCTCCAGCAACACGTGGAAGTGATGACGGAGGAGCGGGGATGATCACCTTCACTGGACACTACTTCATATTGTTGTTCATTTGGGCATGTATGTTGTTTCATTAA

Protein sequence

>Msa0795840

MSIMRMMVVWLIMFVININVISLVDAQMLPPCAGDKMLPCTDYLNSTHPPDICCNPIKEIFEATHDYTCFCQISVPGLLESFASSPGLSPSLLQPPATRGSDDGGAGMITFTGHYFILLFIWACMLFH*