AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0797890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797890 MtrunA17_Chr5g0401401 95.671 231 10 0 1 231 1 231 1.15e-163 451
Msa0797890 MtrunA17_Chr4g0053911 69.710 241 55 6 1 231 1 233 2.44e-109 313
Msa0797890 MtrunA17_Chr1g0207471 56.579 152 61 3 1 147 1 152 5.76e-55 174
Msa0797890 MtrunA17_Chr7g0215281 64.545 110 39 0 1 110 3 112 2.49e-52 171
Msa0797890 MtrunA17_Chr6g0457431 61.818 110 42 0 1 110 3 112 2.35e-50 161
Msa0797890 MtrunA17_Chr6g0469271 46.154 169 80 5 1 164 3 165 2.22e-45 151
Msa0797890 MtrunA17_Chr6g0470651 48.413 126 63 2 1 124 3 128 2.33e-40 138
Msa0797890 MtrunA17_Chr6g0470631 52.294 109 50 2 1 107 3 111 1.75e-38 133
Msa0797890 MtrunA17_Chr1g0209841 50.000 112 38 2 1 107 3 101 1.47e-35 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797890 AT4G37540.1 55.645 248 85 8 1 231 1 240 9.53e-81 242
Msa0797890 AT3G49940.1 57.031 256 76 10 1 231 1 247 4.89e-80 240
Msa0797890 AT5G67420.2 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa0797890 AT5G67420.1 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa0797890 AT1G67100.1 65.455 110 38 0 1 110 3 112 6.52e-52 168
Msa0797890 AT3G02550.1 63.636 110 40 0 1 110 3 112 2.17e-49 162
Msa0797890 AT1G68510.1 57.025 121 50 1 1 119 3 123 4.41e-47 155

Find 102 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 5_3:-10251096 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 5_3:-10250985 None:intergenic
TTAGTAGTATTGAGAAATTT+TGG 0.238378 5_3:+10251732 Msa0797890:three_prime_UTR
ACGCGTTGACCAACGGTTTC+CGG 0.249914 5_3:+10251203 Msa0797890:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 5_3:-10251351 None:intergenic
GTGGCATGACCTTGAGCTTC+AGG 0.279564 5_3:-10250608 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 5_3:-10250547 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 5_3:-10251241 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 5_3:-10251084 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 5_3:-10250899 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 5_3:-10251186 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 5_3:+10250979 Msa0797890:CDS
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 5_3:+10251100 Msa0797890:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 5_3:+10250576 Msa0797890:CDS
AACTAGGGTGCAAGTGCAAA+TGG 0.409490 5_3:-10250387 None:intergenic
AAGTCCGACAGCACCGTTAA+CGG 0.413291 5_3:-10250900 None:intergenic
GGTATAAATAAACACTAGTT+AGG 0.420813 5_3:-10250364 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 5_3:-10250984 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 5_3:+10250989 Msa0797890:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 5_3:+10250535 Msa0797890:CDS
TTACCAGGTCTCTGTGGTGG+AGG 0.463406 5_3:-10250689 None:intergenic
AGAAGAGGAAGAGAAGAGAA+TGG 0.464524 5_3:-10250410 None:intergenic
GGTGGTAAACGCAAGCGATC+AGG 0.465080 5_3:+10251121 Msa0797890:CDS
GAAGAGAAGAGAATGGAACT+AGG 0.469054 5_3:-10250403 None:intergenic
ACTAGTTAGGAGAGAGGAAG+AGG 0.471446 5_3:-10250351 None:intergenic
GCTTCATCGGCAATGAAATC+CGG 0.477949 5_3:+10251460 Msa0797890:three_prime_UTR
TACCACCAGAACTCATGAAC+CGG 0.478203 5_3:-10251105 None:intergenic
ATGAACCGGACTTTCTGGTT+CGG 0.483553 5_3:-10251091 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 5_3:-10250891 None:intergenic
ACTAGGGTGCAAGTGCAAAT+GGG 0.485440 5_3:-10250386 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 5_3:+10251273 Msa0797890:CDS
TGGCATGACCTTGAGCTTCA+GGG 0.492713 5_3:-10250607 None:intergenic
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 5_3:+10251223 Msa0797890:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 5_3:+10250887 Msa0797890:CDS
CGTTGCAACTCATACCTAGT+TGG 0.511859 5_3:-10250514 None:intergenic
GGTGGTAGTGAGGTATGGTT+AGG 0.513227 5_3:-10250305 None:intergenic
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 5_3:+10251222 Msa0797890:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 5_3:+10250946 Msa0797890:CDS
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 5_3:+10250536 Msa0797890:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 5_3:-10250664 None:intergenic
GCGTGTAAACCGAGAAACTC+CGG 0.536983 5_3:-10250998 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 5_3:+10251086 Msa0797890:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 5_3:-10251237 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 5_3:+10251280 Msa0797890:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 5_3:-10250584 None:intergenic
AAGAGAAGAGAATGGAACTA+GGG 0.549109 5_3:-10250402 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 5_3:+10251281 Msa0797890:CDS
AGAATGAGTGGGTGGTAGTG+AGG 0.560089 5_3:-10250315 None:intergenic
TACCAGGTCTCTGTGGTGGA+GGG 0.561251 5_3:-10250688 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 5_3:+10251261 Msa0797890:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 5_3:+10251289 Msa0797890:CDS
TAAACCGAGAAACTCCGGTA+AGG 0.574362 5_3:-10250993 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 5_3:-10251196 None:intergenic
ACACCTCACATGAGGAGAAG+AGG 0.577266 5_3:-10250425 None:intergenic
CTTCCTCTTCTCCTCATGTG+AGG 0.577472 5_3:+10250422 Msa0797890:five_prime_UTR
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 5_3:+10251218 Msa0797890:CDS
AACTAGAGAACGAGAATGAG+TGG 0.578906 5_3:-10250327 None:intergenic
AATTTCCCTCCGGAAACCGT+TGG 0.583258 5_3:-10251212 None:intergenic
AGAGAAAGATGAGGGTTCGT+CGG 0.584589 5_3:+10251537 Msa0797890:three_prime_UTR
ACCGTTGATGACTCATCTGA+AGG 0.585962 5_3:+10251031 Msa0797890:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 5_3:+10251286 Msa0797890:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 5_3:-10251222 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 5_3:+10250896 Msa0797890:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.589079 5_3:-10250992 None:intergenic
TTAAACAGAGAGAAAGATGA+GGG 0.590185 5_3:+10251529 Msa0797890:three_prime_UTR
GTCCCTCCACCACAGAGACC+TGG 0.592710 5_3:+10250686 Msa0797890:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 5_3:-10251163 None:intergenic
TATTTACCAGGTCTCTGTGG+TGG 0.593625 5_3:-10250692 None:intergenic
GAGTGGGTGGTAGTGAGGTA+TGG 0.595919 5_3:-10250310 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 5_3:-10251032 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 5_3:+10251167 Msa0797890:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 5_3:+10251056 Msa0797890:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 5_3:+10251206 Msa0797890:CDS
TTTAAACAGAGAGAAAGATG+AGG 0.602638 5_3:+10251528 Msa0797890:three_prime_UTR
CCACAGAACGCGTTGACCAA+CGG 0.603462 5_3:+10251196 Msa0797890:CDS
CTAGGGTGCAAGTGCAAATG+GGG 0.606285 5_3:-10250385 None:intergenic
AGGGTTTAAGGGTTCCGCCA+CGG 0.607346 5_3:-10250973 None:intergenic
GGCATGACCTTGAGCTTCAG+GGG 0.607961 5_3:-10250606 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 5_3:-10250541 None:intergenic
ATCGATACCCCTGAAGCTCA+AGG 0.611816 5_3:+10250599 Msa0797890:CDS
CAACTAGGTATGAGTTGCAA+CGG 0.621586 5_3:+10250515 Msa0797890:exon
ATAAACACTAGTTAGGAGAG+AGG 0.622037 5_3:-10250357 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 5_3:+10251103 Msa0797890:CDS
TGAAAGACATAAGGTCAGCA+CGG 0.634887 5_3:-10250655 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 5_3:-10250898 None:intergenic
GGTAAACGCAAGCGATCAGG+TGG 0.636761 5_3:+10251124 Msa0797890:CDS
GTTGAAACGGTGCTCCGTGG+CGG 0.644097 5_3:+10250959 Msa0797890:CDS
AAACTGGCACGTGTGTCAAG+CGG 0.645348 5_3:+10250934 Msa0797890:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 5_3:+10251207 Msa0797890:CDS
TGCTAAATACACCTCACATG+AGG 0.652353 5_3:-10250433 None:intergenic
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 5_3:+10250937 Msa0797890:CDS
TTCAGATGAGTCATCAACGG+TGG 0.656087 5_3:-10251029 None:intergenic
GCGGTTGAAACGGTGCTCCG+TGG 0.657002 5_3:+10250956 Msa0797890:CDS
ACTAGAGAACGAGAATGAGT+GGG 0.661667 5_3:-10250326 None:intergenic
GATTATTTACCAGGTCTCTG+TGG 0.665083 5_3:-10250695 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 5_3:+10250863 Msa0797890:CDS
GGTGTGTTACAAAATCTCAC+CGG 0.677482 5_3:-10251479 None:intergenic
GATTCCTCAGAATTCATCGA+CGG 0.685600 5_3:-10251250 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 5_3:-10250583 None:intergenic
AGAGAACGAGAATGAGTGGG+TGG 0.701859 5_3:-10250323 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 5_3:+10251246 Msa0797890:CDS
AAACTTGGCTACAAAGACAG+TGG 0.714204 5_3:-10250627 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_3:10251354-10251373 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - chr5_3:10251671-10251690 None:intergenic 15.0%
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_3:10251354-10251373 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - chr5_3:10251671-10251690 None:intergenic 15.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_3:10250500-10250519 Msa0797890:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_3:10251574-10251593 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_3:10251645-10251664 None:intergenic 20.0%
!!! TTAGTAGTATTGAGAAATTT+TGG + chr5_3:10251732-10251751 Msa0797890:three_prime_UTR 20.0%
!!! TAGTAGTATTGAGAAATTTT+GGG + chr5_3:10251733-10251752 Msa0797890:three_prime_UTR 20.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_3:10250500-10250519 Msa0797890:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_3:10251574-10251593 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_3:10251645-10251664 None:intergenic 20.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_3:10250367-10250386 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_3:10250707-10250726 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_3:10250809-10250828 Msa0797890:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_3:10251384-10251403 Msa0797890:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_3:10251447-10251466 Msa0797890:three_prime_UTR 25.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_3:10250367-10250386 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_3:10250707-10250726 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_3:10250809-10250828 Msa0797890:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_3:10251384-10251403 Msa0797890:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_3:10251447-10251466 Msa0797890:three_prime_UTR 25.0%
TATCGATCCATTGAATACAA+GGG - chr5_3:10250586-10250605 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_3:10250667-10250686 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_3:10251528-10251547 Msa0797890:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_3:10251529-10251548 Msa0797890:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + chr5_3:10251682-10251701 Msa0797890:three_prime_UTR 30.0%
TATCGATCCATTGAATACAA+GGG - chr5_3:10250586-10250605 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_3:10250667-10250686 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_3:10251528-10251547 Msa0797890:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_3:10251529-10251548 Msa0797890:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + chr5_3:10251682-10251701 Msa0797890:three_prime_UTR 30.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_3:10250360-10250379 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_3:10250405-10250424 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_3:10250576-10250595 Msa0797890:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_3:10250587-10250606 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_3:10250632-10250651 Msa0797890:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_3:10250743-10250762 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_3:10251339-10251358 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_3:10251506-10251525 None:intergenic 35.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_3:10250360-10250379 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_3:10250405-10250424 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_3:10250576-10250595 Msa0797890:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_3:10250587-10250606 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_3:10250632-10250651 Msa0797890:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_3:10250743-10250762 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_3:10251339-10251358 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_3:10251506-10251525 None:intergenic 35.0%
ACTAGAGAACGAGAATGAGT+GGG - chr5_3:10250329-10250348 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - chr5_3:10250330-10250349 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_3:10250406-10250425 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_3:10250413-10250432 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_3:10250436-10250455 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_3:10250515-10250534 Msa0797890:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_3:10250544-10250563 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_3:10250550-10250569 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_3:10250630-10250649 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_3:10250658-10250677 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_3:10250698-10250717 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_3:10250863-10250882 Msa0797890:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_3:10250987-10251006 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_3:10251035-10251054 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + chr5_3:10251144-10251163 Msa0797890:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_3:10251253-10251272 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_3:10251482-10251501 None:intergenic 40.0%
ACTAGAGAACGAGAATGAGT+GGG - chr5_3:10250329-10250348 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - chr5_3:10250330-10250349 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_3:10250406-10250425 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_3:10250413-10250432 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_3:10250436-10250455 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_3:10250515-10250534 Msa0797890:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_3:10250544-10250563 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_3:10250550-10250569 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_3:10250630-10250649 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_3:10250658-10250677 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_3:10250698-10250717 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_3:10250863-10250882 Msa0797890:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_3:10250987-10251006 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_3:10251035-10251054 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + chr5_3:10251144-10251163 Msa0797890:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_3:10251253-10251272 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_3:10251482-10251501 None:intergenic 40.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_3:10250354-10250373 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_3:10250389-10250408 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_3:10250390-10250409 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_3:10250517-10250536 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - chr5_3:10250695-10250714 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_3:10250918-10250937 Msa0797890:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_3:10250988-10251007 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_3:10250989-10251008 Msa0797890:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_3:10250995-10251014 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_3:10250996-10251015 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_3:10251032-10251051 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_3:10251031-10251050 Msa0797890:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_3:10251094-10251113 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_3:10251099-10251118 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_3:10251100-10251119 Msa0797890:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_3:10251108-10251127 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_3:10251166-10251185 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_3:10251167-10251186 Msa0797890:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_3:10251246-10251265 Msa0797890:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_3:10251261-10251280 Msa0797890:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_3:10251460-10251479 Msa0797890:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_3:10251487-10251506 Msa0797890:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_3:10251503-10251522 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_3:10251537-10251556 Msa0797890:three_prime_UTR 45.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_3:10250354-10250373 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_3:10250389-10250408 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_3:10250390-10250409 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_3:10250517-10250536 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - chr5_3:10250695-10250714 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_3:10250918-10250937 Msa0797890:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_3:10250988-10251007 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_3:10250989-10251008 Msa0797890:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_3:10250995-10251014 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_3:10250996-10251015 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_3:10251032-10251051 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_3:10251031-10251050 Msa0797890:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_3:10251094-10251113 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_3:10251099-10251118 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_3:10251100-10251119 Msa0797890:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_3:10251108-10251127 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_3:10251166-10251185 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_3:10251167-10251186 Msa0797890:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_3:10251246-10251265 Msa0797890:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_3:10251261-10251280 Msa0797890:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_3:10251460-10251479 Msa0797890:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_3:10251487-10251506 Msa0797890:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_3:10251503-10251522 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_3:10251537-10251556 Msa0797890:three_prime_UTR 45.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_3:10250308-10250327 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - chr5_3:10250318-10250337 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - chr5_3:10250326-10250345 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_3:10250388-10250407 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_3:10250422-10250441 Msa0797890:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_3:10250428-10250447 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_3:10250536-10250555 Msa0797890:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5_3:10250599-10250618 Msa0797890:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5_3:10250610-10250629 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_3:10250887-10250906 Msa0797890:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_3:10250903-10250922 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_3:10250934-10250953 Msa0797890:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_3:10250979-10250998 Msa0797890:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - chr5_3:10251001-10251020 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_3:10251087-10251106 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_3:10251086-10251105 Msa0797890:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_3:10251215-10251234 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_3:10251244-10251263 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_3:10251286-10251305 Msa0797890:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_3:10251499-10251518 Msa0797890:three_prime_UTR 50.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_3:10250308-10250327 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - chr5_3:10250318-10250337 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - chr5_3:10250326-10250345 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_3:10250388-10250407 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_3:10250422-10250441 Msa0797890:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_3:10250428-10250447 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_3:10250536-10250555 Msa0797890:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5_3:10250599-10250618 Msa0797890:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5_3:10250610-10250629 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_3:10250887-10250906 Msa0797890:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_3:10250903-10250922 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_3:10250934-10250953 Msa0797890:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_3:10250979-10250998 Msa0797890:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - chr5_3:10251001-10251020 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_3:10251087-10251106 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_3:10251086-10251105 Msa0797890:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_3:10251215-10251234 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_3:10251244-10251263 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_3:10251286-10251305 Msa0797890:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_3:10251499-10251518 Msa0797890:three_prime_UTR 50.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_3:10250313-10250332 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_3:10250535-10250554 Msa0797890:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5_3:10250609-10250628 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5_3:10250611-10250630 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_3:10250645-10250664 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - chr5_3:10250691-10250710 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - chr5_3:10250692-10250711 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_3:10250894-10250913 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_3:10250896-10250915 Msa0797890:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_3:10250902-10250921 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_3:10250906-10250925 Msa0797890:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_3:10250911-10250930 Msa0797890:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_3:10250946-10250965 Msa0797890:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_3:10250976-10250995 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_3:10251103-10251122 Msa0797890:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_3:10251121-10251140 Msa0797890:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + chr5_3:10251124-10251143 Msa0797890:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_3:10251189-10251208 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_3:10251196-10251215 Msa0797890:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_3:10251203-10251222 Msa0797890:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_3:10251207-10251226 Msa0797890:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_3:10251218-10251237 Msa0797890:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_3:10251280-10251299 Msa0797890:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_3:10251281-10251300 Msa0797890:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_3:10251289-10251308 Msa0797890:CDS 55.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_3:10250313-10250332 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_3:10250535-10250554 Msa0797890:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5_3:10250609-10250628 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5_3:10250611-10250630 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_3:10250645-10250664 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - chr5_3:10250691-10250710 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - chr5_3:10250692-10250711 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_3:10250894-10250913 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_3:10250896-10250915 Msa0797890:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_3:10250902-10250921 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_3:10250906-10250925 Msa0797890:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_3:10250911-10250930 Msa0797890:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_3:10250946-10250965 Msa0797890:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_3:10250976-10250995 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_3:10251103-10251122 Msa0797890:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_3:10251121-10251140 Msa0797890:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + chr5_3:10251124-10251143 Msa0797890:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_3:10251189-10251208 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_3:10251196-10251215 Msa0797890:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_3:10251203-10251222 Msa0797890:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_3:10251207-10251226 Msa0797890:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_3:10251218-10251237 Msa0797890:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_3:10251280-10251299 Msa0797890:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_3:10251281-10251300 Msa0797890:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_3:10251289-10251308 Msa0797890:CDS 55.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_3:10250901-10250920 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_3:10250959-10250978 Msa0797890:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_3:10251056-10251075 Msa0797890:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_3:10251199-10251218 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_3:10251206-10251225 Msa0797890:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_3:10251273-10251292 Msa0797890:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_3:10250901-10250920 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_3:10250959-10250978 Msa0797890:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_3:10251056-10251075 Msa0797890:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_3:10251199-10251218 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_3:10251206-10251225 Msa0797890:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_3:10251273-10251292 Msa0797890:CDS 60.0%
GTCCCTCCACCACAGAGACC+TGG + chr5_3:10250686-10250705 Msa0797890:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_3:10250937-10250956 Msa0797890:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_3:10250956-10250975 Msa0797890:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_3:10251240-10251259 None:intergenic 65.0%
GTCCCTCCACCACAGAGACC+TGG + chr5_3:10250686-10250705 Msa0797890:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_3:10250937-10250956 Msa0797890:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_3:10250956-10250975 Msa0797890:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_3:10251240-10251259 None:intergenic 65.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_3:10251225-10251244 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_3:10251222-10251241 Msa0797890:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_3:10251223-10251242 Msa0797890:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_3:10251225-10251244 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_3:10251222-10251241 Msa0797890:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_3:10251223-10251242 Msa0797890:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 10250298 10251754 10250298 ID=Msa0797890;Name=Msa0797890
chr5_3 mRNA 10250298 10251754 10250298 ID=Msa0797890-mRNA-1;Parent=Msa0797890;Name=Msa0797890-mRNA-1;_AED=0.05;_eAED=0.05;_QI=226|1|1|1|1|1|2|391|231
chr5_3 exon 10250298 10250707 10250298 ID=Msa0797890-mRNA-1:exon:4320;Parent=Msa0797890-mRNA-1
chr5_3 exon 10250852 10251754 10250852 ID=Msa0797890-mRNA-1:exon:4321;Parent=Msa0797890-mRNA-1
chr5_3 five_prime_UTR 10250298 10250523 10250298 ID=Msa0797890-mRNA-1:five_prime_utr;Parent=Msa0797890-mRNA-1
chr5_3 CDS 10250524 10250707 10250524 ID=Msa0797890-mRNA-1:cds;Parent=Msa0797890-mRNA-1
chr5_3 CDS 10250852 10251363 10250852 ID=Msa0797890-mRNA-1:cds;Parent=Msa0797890-mRNA-1
chr5_3 three_prime_UTR 10251364 10251754 10251364 ID=Msa0797890-mRNA-1:three_prime_utr;Parent=Msa0797890-mRNA-1
Gene Sequence

>Msa0797890

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGAAGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTCCACCACAGAGACCTGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCCGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTACACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGGTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGAAGACCGCAAAGTTCTCAACCTTTTTGTTTGA
Protein sequence

>Msa0797890

MSCNGCRVLRKGCSESCILRPCIQWIDTPEAQGHATVFVAKFFGRADLMSFISNVPPPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLKPLPEFLGLHALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGGEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDEDRKVLNLFV*