AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0835450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835450 MtrunA17_Chr5g0401401 95.671 231 10 0 1 231 1 231 9.65e-165 454
Msa0835450 MtrunA17_Chr4g0053911 70.124 241 54 6 1 231 1 233 5.43e-112 320
Msa0835450 MtrunA17_Chr1g0207471 56.579 152 61 3 1 147 1 152 1.21e-54 173
Msa0835450 MtrunA17_Chr7g0215281 63.636 110 40 0 1 110 3 112 3.40e-52 171
Msa0835450 MtrunA17_Chr6g0457431 52.899 138 55 1 1 128 3 140 1.71e-50 162
Msa0835450 MtrunA17_Chr6g0469271 51.880 133 60 2 1 131 3 133 1.87e-45 151
Msa0835450 MtrunA17_Chr6g0470651 47.368 133 66 3 1 131 3 133 2.65e-40 138
Msa0835450 MtrunA17_Chr6g0470631 46.617 133 67 3 1 131 3 133 3.38e-39 135
Msa0835450 MtrunA17_Chr1g0209841 45.865 133 58 3 1 131 3 123 3.08e-36 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835450 AT4G37540.1 56.048 248 84 8 1 231 1 240 2.52e-82 246
Msa0835450 AT3G49940.1 56.641 256 77 10 1 231 1 247 2.23e-80 241
Msa0835450 AT5G67420.2 53.333 255 90 6 1 231 1 250 3.46e-76 231
Msa0835450 AT5G67420.1 53.333 255 90 6 1 231 1 250 3.46e-76 231
Msa0835450 AT1G67100.1 64.545 110 39 0 1 110 3 112 9.03e-52 167
Msa0835450 AT3G02550.1 62.727 110 41 0 1 110 3 112 2.29e-49 162
Msa0835450 AT1G68510.1 61.062 113 42 1 1 111 3 115 1.98e-47 157

Find 103 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 5_4:-8796840 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 5_4:-8796729 None:intergenic
GCAGTAGTATTGAGAAATTT+TGG 0.201017 5_4:+8797485 Msa0835450:three_prime_UTR
ACGCGTTGACCAACGGTTTC+CGG 0.249914 5_4:+8796947 Msa0835450:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 5_4:-8797095 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 5_4:-8796293 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 5_4:-8796985 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 5_4:-8796828 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 5_4:-8796643 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 5_4:-8796930 None:intergenic
GTGGCATGACCTTGAGCATC+AGG 0.382282 5_4:-8796354 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 5_4:+8796723 Msa0835450:CDS
AGTAGTAGTTATTTATCAAA+AGG 0.387469 5_4:+8797454 Msa0835450:three_prime_UTR
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 5_4:+8796844 Msa0835450:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 5_4:+8796322 Msa0835450:CDS
AACTAGGGTGCAAGTGCAAA+TGG 0.409490 5_4:-8796133 None:intergenic
AAGTCCGACAGCACCGTTAA+CGG 0.413291 5_4:-8796644 None:intergenic
GGTATAAATAAACACTAGTT+AGG 0.420813 5_4:-8796110 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 5_4:-8796728 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 5_4:+8796733 Msa0835450:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 5_4:+8796281 Msa0835450:CDS
AGAAGAGGAAGAGAAGAGAA+TGG 0.464524 5_4:-8796156 None:intergenic
GGTGGTAAACGCAAGCGATC+AGG 0.465080 5_4:+8796865 Msa0835450:CDS
GAAGAGAAGAGAATGGAACT+AGG 0.469054 5_4:-8796149 None:intergenic
ACTAGTTAGGAGAGAGGAAG+AGG 0.471446 5_4:-8796097 None:intergenic
GCTTCATCGGCAATGAAATC+CGG 0.477949 5_4:+8797204 Msa0835450:three_prime_UTR
TACCACCAGAACTCATGAAC+CGG 0.478203 5_4:-8796849 None:intergenic
TTATGGATCGTGCAATTAAG+AGG 0.483356 5_4:+8797145 Msa0835450:three_prime_UTR
ATGAACCGGACTTTCTGGTT+CGG 0.483553 5_4:-8796835 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 5_4:-8796635 None:intergenic
ACTAGGGTGCAAGTGCAAAT+GGG 0.485440 5_4:-8796132 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 5_4:+8797017 Msa0835450:CDS
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 5_4:+8796967 Msa0835450:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 5_4:+8796631 Msa0835450:CDS
CGTTGCAACTCATACCTAGT+TGG 0.511859 5_4:-8796260 None:intergenic
GGTGGTAGTGAGGTATGGTT+AGG 0.513227 5_4:-8796051 None:intergenic
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 5_4:+8796966 Msa0835450:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 5_4:+8796690 Msa0835450:CDS
TTACCAGGTCTCTGTGGTGA+AGG 0.520683 5_4:-8796435 None:intergenic
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 5_4:+8796282 Msa0835450:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 5_4:-8796410 None:intergenic
GCGTCTAAACCGAGAAACTC+CGG 0.534500 5_4:-8796742 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 5_4:+8796830 Msa0835450:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 5_4:-8796981 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 5_4:+8797024 Msa0835450:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 5_4:-8796330 None:intergenic
AAGAGAAGAGAATGGAACTA+GGG 0.549109 5_4:-8796148 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 5_4:+8797025 Msa0835450:CDS
TACCAGGTCTCTGTGGTGAA+GGG 0.560196 5_4:-8796434 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 5_4:+8797005 Msa0835450:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 5_4:+8797033 Msa0835450:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.570584 5_4:-8796736 None:intergenic
TAAACCGAGAAACTCCGGTA+AGG 0.571879 5_4:-8796737 None:intergenic
GAGTGGGTGGTAGTGAGGTA+TGG 0.575021 5_4:-8796056 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 5_4:-8796940 None:intergenic
ACACCTCACATGAGGAGAAG+AGG 0.577266 5_4:-8796171 None:intergenic
CTTCCTCTTCTCCTCATGTG+AGG 0.577472 5_4:+8796168 Msa0835450:five_prime_UTR
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 5_4:+8796962 Msa0835450:CDS
AATTTCCCTCCGGAAACCGT+TGG 0.583258 5_4:-8796956 None:intergenic
AGAGAAAGATGAGGGTTCGT+CGG 0.584589 5_4:+8797281 Msa0835450:three_prime_UTR
ACCGTTGATGACTCATCTGA+AGG 0.585962 5_4:+8796775 Msa0835450:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 5_4:+8797030 Msa0835450:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 5_4:-8796966 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 5_4:+8796640 Msa0835450:CDS
TTAAACAGAGAGAAAGATGA+GGG 0.590185 5_4:+8797273 Msa0835450:three_prime_UTR
TGGCATGACCTTGAGCATCA+GGG 0.592011 5_4:-8796353 None:intergenic
GTCCCTTCACCACAGAGACC+TGG 0.592710 5_4:+8796432 Msa0835450:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 5_4:-8796907 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 5_4:-8796776 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 5_4:+8796911 Msa0835450:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 5_4:+8796800 Msa0835450:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 5_4:+8796950 Msa0835450:CDS
TTTAAACAGAGAGAAAGATG+AGG 0.602638 5_4:+8797272 Msa0835450:three_prime_UTR
CCACAGAACGCGTTGACCAA+CGG 0.603462 5_4:+8796940 Msa0835450:CDS
ATCGATACCCCTGATGCTCA+AGG 0.605725 5_4:+8796345 Msa0835450:CDS
CTAGGGTGCAAGTGCAAATG+GGG 0.606285 5_4:-8796131 None:intergenic
AGGGTTTAAGGGTTCCGCCA+CGG 0.607346 5_4:-8796717 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 5_4:-8796287 None:intergenic
AACTAGAGAACGAGACTGAG+TGG 0.615527 5_4:-8796073 None:intergenic
CAACTAGGTATGAGTTGCAA+CGG 0.621586 5_4:+8796261 Msa0835450:exon
ATAAACACTAGTTAGGAGAG+AGG 0.622037 5_4:-8796103 None:intergenic
GGCATGACCTTGAGCATCAG+GGG 0.622526 5_4:-8796352 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 5_4:+8796847 Msa0835450:CDS
AGACTGAGTGGGTGGTAGTG+AGG 0.633551 5_4:-8796061 None:intergenic
TGAAAGACATAAGGTCAGCA+CGG 0.634887 5_4:-8796401 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 5_4:-8796642 None:intergenic
GTTGAAACGGTGCTCCGTGG+CGG 0.644097 5_4:+8796703 Msa0835450:CDS
AAACTGGCACGTGTGTCAAG+CGG 0.645348 5_4:+8796678 Msa0835450:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 5_4:+8796951 Msa0835450:CDS
TGCTAAATACACCTCACATG+AGG 0.652353 5_4:-8796179 None:intergenic
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 5_4:+8796681 Msa0835450:CDS
ACTAGAGAACGAGACTGAGT+GGG 0.654460 5_4:-8796072 None:intergenic
TTCAGATGAGTCATCAACGG+TGG 0.656087 5_4:-8796773 None:intergenic
GCGGTTGAAACGGTGCTCCG+TGG 0.657002 5_4:+8796700 Msa0835450:CDS
GATTATTTACCAGGTCTCTG+TGG 0.665083 5_4:-8796441 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 5_4:+8796607 Msa0835450:CDS
GGTGTGTTACAAAATCTCAC+CGG 0.677482 5_4:-8797223 None:intergenic
GATTCCTCAGAATTCATCGA+CGG 0.685600 5_4:-8796994 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 5_4:-8796329 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 5_4:+8796990 Msa0835450:CDS
AGAGAACGAGACTGAGTGGG+TGG 0.705977 5_4:-8796069 None:intergenic
AAACTTGGCTACAAAGACAG+TGG 0.714204 5_4:-8796373 None:intergenic
GGAGATAACTACGTTCACGA+CGG 0.740228 5_4:+8797054 Msa0835450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_4:8797098-8797117 None:intergenic 15.0%
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_4:8797098-8797117 None:intergenic 15.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_4:8796246-8796265 Msa0835450:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_4:8797318-8797337 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_4:8797389-8797408 None:intergenic 20.0%
!! AGTAGTAGTTATTTATCAAA+AGG + chr5_4:8797454-8797473 Msa0835450:three_prime_UTR 20.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_4:8796246-8796265 Msa0835450:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_4:8797318-8797337 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_4:8797389-8797408 None:intergenic 20.0%
!! AGTAGTAGTTATTTATCAAA+AGG + chr5_4:8797454-8797473 Msa0835450:three_prime_UTR 20.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_4:8796113-8796132 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_4:8796453-8796472 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_4:8796553-8796572 Msa0835450:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_4:8797128-8797147 Msa0835450:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_4:8797191-8797210 Msa0835450:three_prime_UTR 25.0%
!!! CAGTAGTATTGAGAAATTTT+GGG + chr5_4:8797486-8797505 Msa0835450:three_prime_UTR 25.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_4:8796113-8796132 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_4:8796453-8796472 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_4:8796553-8796572 Msa0835450:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_4:8797128-8797147 Msa0835450:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_4:8797191-8797210 Msa0835450:three_prime_UTR 25.0%
TATCGATCCATTGAATACAA+GGG - chr5_4:8796332-8796351 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_4:8796413-8796432 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_4:8797272-8797291 Msa0835450:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_4:8797273-8797292 Msa0835450:three_prime_UTR 30.0%
! GTTTTTGTTAGAGTCTAGTT+TGG + chr5_4:8797428-8797447 Msa0835450:three_prime_UTR 30.0%
!! GCAGTAGTATTGAGAAATTT+TGG + chr5_4:8797485-8797504 Msa0835450:three_prime_UTR 30.0%
TATCGATCCATTGAATACAA+GGG - chr5_4:8796332-8796351 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_4:8796413-8796432 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_4:8797272-8797291 Msa0835450:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_4:8797273-8797292 Msa0835450:three_prime_UTR 30.0%
! GTTTTTGTTAGAGTCTAGTT+TGG + chr5_4:8797428-8797447 Msa0835450:three_prime_UTR 30.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_4:8796106-8796125 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_4:8796151-8796170 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_4:8796322-8796341 Msa0835450:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_4:8796333-8796352 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_4:8796378-8796397 Msa0835450:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_4:8796487-8796506 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_4:8797083-8797102 None:intergenic 35.0%
TTATGGATCGTGCAATTAAG+AGG + chr5_4:8797145-8797164 Msa0835450:three_prime_UTR 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_4:8797250-8797269 None:intergenic 35.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_4:8796106-8796125 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_4:8796151-8796170 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_4:8796322-8796341 Msa0835450:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_4:8796333-8796352 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_4:8796378-8796397 Msa0835450:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_4:8796487-8796506 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_4:8797083-8797102 None:intergenic 35.0%
TTATGGATCGTGCAATTAAG+AGG + chr5_4:8797145-8797164 Msa0835450:three_prime_UTR 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_4:8797250-8797269 None:intergenic 35.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_4:8796152-8796171 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_4:8796159-8796178 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_4:8796182-8796201 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_4:8796261-8796280 Msa0835450:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_4:8796290-8796309 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_4:8796296-8796315 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_4:8796376-8796395 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_4:8796404-8796423 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_4:8796444-8796463 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_4:8796607-8796626 Msa0835450:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_4:8796731-8796750 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_4:8796779-8796798 None:intergenic 40.0%
!!! TGCTGAAGTTTTGAAGCTTC+CGG + chr5_4:8796888-8796907 Msa0835450:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_4:8796997-8797016 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_4:8797226-8797245 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_4:8796152-8796171 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_4:8796159-8796178 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_4:8796182-8796201 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_4:8796261-8796280 Msa0835450:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_4:8796290-8796309 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_4:8796296-8796315 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_4:8796376-8796395 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_4:8796404-8796423 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_4:8796444-8796463 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_4:8796607-8796626 Msa0835450:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_4:8796731-8796750 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_4:8796779-8796798 None:intergenic 40.0%
!!! TGCTGAAGTTTTGAAGCTTC+CGG + chr5_4:8796888-8796907 Msa0835450:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_4:8796997-8797016 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_4:8797226-8797245 None:intergenic 40.0%
ACTAGAGAACGAGACTGAGT+GGG - chr5_4:8796075-8796094 None:intergenic 45.0%
AACTAGAGAACGAGACTGAG+TGG - chr5_4:8796076-8796095 None:intergenic 45.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_4:8796100-8796119 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_4:8796135-8796154 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_4:8796136-8796155 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_4:8796263-8796282 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_4:8796662-8796681 Msa0835450:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_4:8796732-8796751 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_4:8796733-8796752 Msa0835450:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_4:8796739-8796758 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_4:8796740-8796759 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_4:8796776-8796795 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_4:8796775-8796794 Msa0835450:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_4:8796838-8796857 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_4:8796843-8796862 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_4:8796844-8796863 Msa0835450:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_4:8796852-8796871 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_4:8796910-8796929 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_4:8796911-8796930 Msa0835450:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_4:8796990-8797009 Msa0835450:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_4:8797005-8797024 Msa0835450:CDS 45.0%
GGAGATAACTACGTTCACGA+CGG + chr5_4:8797054-8797073 Msa0835450:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_4:8797204-8797223 Msa0835450:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_4:8797231-8797250 Msa0835450:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_4:8797247-8797266 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_4:8797281-8797300 Msa0835450:three_prime_UTR 45.0%
ACTAGAGAACGAGACTGAGT+GGG - chr5_4:8796075-8796094 None:intergenic 45.0%
AACTAGAGAACGAGACTGAG+TGG - chr5_4:8796076-8796095 None:intergenic 45.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_4:8796100-8796119 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_4:8796135-8796154 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_4:8796136-8796155 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_4:8796263-8796282 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_4:8796662-8796681 Msa0835450:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_4:8796732-8796751 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_4:8796733-8796752 Msa0835450:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_4:8796739-8796758 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_4:8796740-8796759 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_4:8796776-8796795 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_4:8796775-8796794 Msa0835450:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_4:8796838-8796857 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_4:8796843-8796862 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_4:8796844-8796863 Msa0835450:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_4:8796852-8796871 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_4:8796910-8796929 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_4:8796911-8796930 Msa0835450:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_4:8796990-8797009 Msa0835450:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_4:8797005-8797024 Msa0835450:CDS 45.0%
GGAGATAACTACGTTCACGA+CGG + chr5_4:8797054-8797073 Msa0835450:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_4:8797204-8797223 Msa0835450:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_4:8797231-8797250 Msa0835450:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_4:8797247-8797266 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_4:8797281-8797300 Msa0835450:three_prime_UTR 45.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_4:8796054-8796073 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_4:8796134-8796153 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_4:8796168-8796187 Msa0835450:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_4:8796174-8796193 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_4:8796282-8796301 Msa0835450:CDS 50.0%
ATCGATACCCCTGATGCTCA+AGG + chr5_4:8796345-8796364 Msa0835450:CDS 50.0%
TGGCATGACCTTGAGCATCA+GGG - chr5_4:8796356-8796375 None:intergenic 50.0%
TACCAGGTCTCTGTGGTGAA+GGG - chr5_4:8796437-8796456 None:intergenic 50.0%
TTACCAGGTCTCTGTGGTGA+AGG - chr5_4:8796438-8796457 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_4:8796631-8796650 Msa0835450:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_4:8796647-8796666 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_4:8796678-8796697 Msa0835450:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_4:8796723-8796742 Msa0835450:CDS 50.0%
GCGTCTAAACCGAGAAACTC+CGG - chr5_4:8796745-8796764 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_4:8796831-8796850 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_4:8796830-8796849 Msa0835450:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_4:8796959-8796978 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_4:8796988-8797007 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_4:8797030-8797049 Msa0835450:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_4:8797243-8797262 Msa0835450:three_prime_UTR 50.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_4:8796054-8796073 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_4:8796134-8796153 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_4:8796168-8796187 Msa0835450:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_4:8796174-8796193 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_4:8796282-8796301 Msa0835450:CDS 50.0%
ATCGATACCCCTGATGCTCA+AGG + chr5_4:8796345-8796364 Msa0835450:CDS 50.0%
TGGCATGACCTTGAGCATCA+GGG - chr5_4:8796356-8796375 None:intergenic 50.0%
TACCAGGTCTCTGTGGTGAA+GGG - chr5_4:8796437-8796456 None:intergenic 50.0%
TTACCAGGTCTCTGTGGTGA+AGG - chr5_4:8796438-8796457 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_4:8796631-8796650 Msa0835450:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_4:8796647-8796666 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_4:8796678-8796697 Msa0835450:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_4:8796723-8796742 Msa0835450:CDS 50.0%
GCGTCTAAACCGAGAAACTC+CGG - chr5_4:8796745-8796764 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_4:8796831-8796850 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_4:8796830-8796849 Msa0835450:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_4:8796959-8796978 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_4:8796988-8797007 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_4:8797030-8797049 Msa0835450:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_4:8797243-8797262 Msa0835450:three_prime_UTR 50.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_4:8796059-8796078 None:intergenic 55.0%
! AGACTGAGTGGGTGGTAGTG+AGG - chr5_4:8796064-8796083 None:intergenic 55.0%
AGAGAACGAGACTGAGTGGG+TGG - chr5_4:8796072-8796091 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_4:8796281-8796300 Msa0835450:CDS 55.0%
GGCATGACCTTGAGCATCAG+GGG - chr5_4:8796355-8796374 None:intergenic 55.0%
GTGGCATGACCTTGAGCATC+AGG - chr5_4:8796357-8796376 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_4:8796391-8796410 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_4:8796638-8796657 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_4:8796640-8796659 Msa0835450:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_4:8796646-8796665 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_4:8796650-8796669 Msa0835450:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_4:8796655-8796674 Msa0835450:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_4:8796690-8796709 Msa0835450:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_4:8796720-8796739 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_4:8796847-8796866 Msa0835450:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_4:8796865-8796884 Msa0835450:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_4:8796933-8796952 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_4:8796940-8796959 Msa0835450:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_4:8796947-8796966 Msa0835450:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_4:8796951-8796970 Msa0835450:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_4:8796962-8796981 Msa0835450:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_4:8797024-8797043 Msa0835450:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_4:8797025-8797044 Msa0835450:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_4:8797033-8797052 Msa0835450:CDS 55.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_4:8796059-8796078 None:intergenic 55.0%
! AGACTGAGTGGGTGGTAGTG+AGG - chr5_4:8796064-8796083 None:intergenic 55.0%
AGAGAACGAGACTGAGTGGG+TGG - chr5_4:8796072-8796091 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_4:8796281-8796300 Msa0835450:CDS 55.0%
GGCATGACCTTGAGCATCAG+GGG - chr5_4:8796355-8796374 None:intergenic 55.0%
GTGGCATGACCTTGAGCATC+AGG - chr5_4:8796357-8796376 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_4:8796391-8796410 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_4:8796638-8796657 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_4:8796640-8796659 Msa0835450:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_4:8796646-8796665 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_4:8796650-8796669 Msa0835450:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_4:8796655-8796674 Msa0835450:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_4:8796690-8796709 Msa0835450:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_4:8796720-8796739 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_4:8796847-8796866 Msa0835450:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_4:8796865-8796884 Msa0835450:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_4:8796933-8796952 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_4:8796940-8796959 Msa0835450:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_4:8796947-8796966 Msa0835450:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_4:8796951-8796970 Msa0835450:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_4:8796962-8796981 Msa0835450:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_4:8797024-8797043 Msa0835450:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_4:8797025-8797044 Msa0835450:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_4:8797033-8797052 Msa0835450:CDS 55.0%
GTCCCTTCACCACAGAGACC+TGG + chr5_4:8796432-8796451 Msa0835450:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_4:8796645-8796664 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_4:8796703-8796722 Msa0835450:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_4:8796800-8796819 Msa0835450:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_4:8796943-8796962 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_4:8796950-8796969 Msa0835450:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_4:8797017-8797036 Msa0835450:CDS 60.0%
GTCCCTTCACCACAGAGACC+TGG + chr5_4:8796432-8796451 Msa0835450:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_4:8796645-8796664 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_4:8796703-8796722 Msa0835450:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_4:8796800-8796819 Msa0835450:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_4:8796943-8796962 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_4:8796950-8796969 Msa0835450:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_4:8797017-8797036 Msa0835450:CDS 60.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_4:8796681-8796700 Msa0835450:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_4:8796700-8796719 Msa0835450:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_4:8796984-8797003 None:intergenic 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_4:8796681-8796700 Msa0835450:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_4:8796700-8796719 Msa0835450:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_4:8796984-8797003 None:intergenic 65.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_4:8796969-8796988 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_4:8796966-8796985 Msa0835450:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_4:8796967-8796986 Msa0835450:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_4:8796969-8796988 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_4:8796966-8796985 Msa0835450:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_4:8796967-8796986 Msa0835450:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 8796044 8797507 8796044 ID=Msa0835450;Name=Msa0835450
chr5_4 mRNA 8796044 8797507 8796044 ID=Msa0835450-mRNA-1;Parent=Msa0835450;Name=Msa0835450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=226|1|1|1|1|1|2|400|231
chr5_4 exon 8796044 8796453 8796044 ID=Msa0835450-mRNA-1:exon:3875;Parent=Msa0835450-mRNA-1
chr5_4 exon 8796596 8797507 8796596 ID=Msa0835450-mRNA-1:exon:3876;Parent=Msa0835450-mRNA-1
chr5_4 five_prime_UTR 8796044 8796269 8796044 ID=Msa0835450-mRNA-1:five_prime_utr;Parent=Msa0835450-mRNA-1
chr5_4 CDS 8796270 8796453 8796270 ID=Msa0835450-mRNA-1:cds;Parent=Msa0835450-mRNA-1
chr5_4 CDS 8796596 8797107 8796596 ID=Msa0835450-mRNA-1:cds;Parent=Msa0835450-mRNA-1
chr5_4 three_prime_UTR 8797108 8797507 8797108 ID=Msa0835450-mRNA-1:three_prime_utr;Parent=Msa0835450-mRNA-1
Gene Sequence

>Msa0835450

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGATGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTTCACCACAGAGACCTGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCCGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTAGACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGCTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGGAGACCGCAAAGTTCTCAACCTTTTTGTTTGA
Protein sequence

>Msa0835450

MSCNGCRVLRKGCSESCILRPCIQWIDTPDAQGHATVFVAKFFGRADLMSFISNVPSPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLKPLPEFLGLDALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGAEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDGDRKVLNLFV*