AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0714100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714100 MtrunA17_Chr5g0401401 95.671 231 10 0 1 231 1 231 1.15e-163 451
Msa0714100 MtrunA17_Chr4g0053911 69.710 241 55 6 1 231 1 233 2.44e-109 313
Msa0714100 MtrunA17_Chr1g0207471 56.579 152 61 3 1 147 1 152 5.76e-55 174
Msa0714100 MtrunA17_Chr7g0215281 64.545 110 39 0 1 110 3 112 2.49e-52 171
Msa0714100 MtrunA17_Chr6g0457431 61.818 110 42 0 1 110 3 112 2.35e-50 161
Msa0714100 MtrunA17_Chr6g0469271 46.154 169 80 5 1 164 3 165 2.22e-45 151
Msa0714100 MtrunA17_Chr6g0470651 48.413 126 63 2 1 124 3 128 2.33e-40 138
Msa0714100 MtrunA17_Chr6g0470631 52.294 109 50 2 1 107 3 111 1.75e-38 133
Msa0714100 MtrunA17_Chr1g0209841 50.000 112 38 2 1 107 3 101 1.47e-35 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714100 AT4G37540.1 55.645 248 85 8 1 231 1 240 9.53e-81 242
Msa0714100 AT3G49940.1 57.031 256 76 10 1 231 1 247 4.89e-80 240
Msa0714100 AT5G67420.2 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa0714100 AT5G67420.1 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa0714100 AT1G67100.1 65.455 110 38 0 1 110 3 112 6.52e-52 168
Msa0714100 AT3G02550.1 63.636 110 40 0 1 110 3 112 2.17e-49 162
Msa0714100 AT1G68510.1 57.025 121 50 1 1 119 3 123 4.41e-47 155

Find 102 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 5_1:-6706094 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 5_1:-6705983 None:intergenic
TTAGTAGTATTGAGAAATTT+TGG 0.238378 5_1:+6706730 Msa0714100:three_prime_UTR
ACGCGTTGACCAACGGTTTC+CGG 0.249914 5_1:+6706201 Msa0714100:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 5_1:-6706349 None:intergenic
GTGGCATGACCTTGAGCTTC+AGG 0.279564 5_1:-6705606 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 5_1:-6705545 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 5_1:-6706239 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 5_1:-6706082 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 5_1:-6705897 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 5_1:-6706184 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 5_1:+6705977 Msa0714100:CDS
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 5_1:+6706098 Msa0714100:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 5_1:+6705574 Msa0714100:CDS
AACTAGGGTGCAAGTGCAAA+TGG 0.409490 5_1:-6705385 None:intergenic
AAGTCCGACAGCACCGTTAA+CGG 0.413291 5_1:-6705898 None:intergenic
GGTATAAATAAACACTAGTT+AGG 0.420813 5_1:-6705362 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 5_1:-6705982 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 5_1:+6705987 Msa0714100:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 5_1:+6705533 Msa0714100:CDS
TTACCAGGTCTCTGTGGTGG+AGG 0.463406 5_1:-6705687 None:intergenic
AGAAGAGGAAGAGAAGAGAA+TGG 0.464524 5_1:-6705408 None:intergenic
GGTGGTAAACGCAAGCGATC+AGG 0.465080 5_1:+6706119 Msa0714100:CDS
GAAGAGAAGAGAATGGAACT+AGG 0.469054 5_1:-6705401 None:intergenic
ACTAGTTAGGAGAGAGGAAG+AGG 0.471446 5_1:-6705349 None:intergenic
GCTTCATCGGCAATGAAATC+CGG 0.477949 5_1:+6706458 Msa0714100:three_prime_UTR
TACCACCAGAACTCATGAAC+CGG 0.478203 5_1:-6706103 None:intergenic
ATGAACCGGACTTTCTGGTT+CGG 0.483553 5_1:-6706089 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 5_1:-6705889 None:intergenic
ACTAGGGTGCAAGTGCAAAT+GGG 0.485440 5_1:-6705384 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 5_1:+6706271 Msa0714100:CDS
TGGCATGACCTTGAGCTTCA+GGG 0.492713 5_1:-6705605 None:intergenic
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 5_1:+6706221 Msa0714100:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 5_1:+6705885 Msa0714100:CDS
CGTTGCAACTCATACCTAGT+TGG 0.511859 5_1:-6705512 None:intergenic
GGTGGTAGTGAGGTATGGTT+AGG 0.513227 5_1:-6705303 None:intergenic
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 5_1:+6706220 Msa0714100:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 5_1:+6705944 Msa0714100:CDS
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 5_1:+6705534 Msa0714100:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 5_1:-6705662 None:intergenic
GCGTGTAAACCGAGAAACTC+CGG 0.536983 5_1:-6705996 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 5_1:+6706084 Msa0714100:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 5_1:-6706235 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 5_1:+6706278 Msa0714100:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 5_1:-6705582 None:intergenic
AAGAGAAGAGAATGGAACTA+GGG 0.549109 5_1:-6705400 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 5_1:+6706279 Msa0714100:CDS
AGAATGAGTGGGTGGTAGTG+AGG 0.560089 5_1:-6705313 None:intergenic
TACCAGGTCTCTGTGGTGGA+GGG 0.561251 5_1:-6705686 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 5_1:+6706259 Msa0714100:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 5_1:+6706287 Msa0714100:CDS
TAAACCGAGAAACTCCGGTA+AGG 0.574362 5_1:-6705991 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 5_1:-6706194 None:intergenic
ACACCTCACATGAGGAGAAG+AGG 0.577266 5_1:-6705423 None:intergenic
CTTCCTCTTCTCCTCATGTG+AGG 0.577472 5_1:+6705420 Msa0714100:five_prime_UTR
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 5_1:+6706216 Msa0714100:CDS
AACTAGAGAACGAGAATGAG+TGG 0.578906 5_1:-6705325 None:intergenic
AATTTCCCTCCGGAAACCGT+TGG 0.583258 5_1:-6706210 None:intergenic
AGAGAAAGATGAGGGTTCGT+CGG 0.584589 5_1:+6706535 Msa0714100:three_prime_UTR
ACCGTTGATGACTCATCTGA+AGG 0.585962 5_1:+6706029 Msa0714100:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 5_1:+6706284 Msa0714100:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 5_1:-6706220 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 5_1:+6705894 Msa0714100:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.589079 5_1:-6705990 None:intergenic
TTAAACAGAGAGAAAGATGA+GGG 0.590185 5_1:+6706527 Msa0714100:three_prime_UTR
GTCCCTCCACCACAGAGACC+TGG 0.592710 5_1:+6705684 Msa0714100:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 5_1:-6706161 None:intergenic
TATTTACCAGGTCTCTGTGG+TGG 0.593625 5_1:-6705690 None:intergenic
GAGTGGGTGGTAGTGAGGTA+TGG 0.595919 5_1:-6705308 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 5_1:-6706030 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 5_1:+6706165 Msa0714100:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 5_1:+6706054 Msa0714100:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 5_1:+6706204 Msa0714100:CDS
TTTAAACAGAGAGAAAGATG+AGG 0.602638 5_1:+6706526 Msa0714100:three_prime_UTR
CCACAGAACGCGTTGACCAA+CGG 0.603462 5_1:+6706194 Msa0714100:CDS
CTAGGGTGCAAGTGCAAATG+GGG 0.606285 5_1:-6705383 None:intergenic
AGGGTTTAAGGGTTCCGCCA+CGG 0.607346 5_1:-6705971 None:intergenic
GGCATGACCTTGAGCTTCAG+GGG 0.607961 5_1:-6705604 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 5_1:-6705539 None:intergenic
ATCGATACCCCTGAAGCTCA+AGG 0.611816 5_1:+6705597 Msa0714100:CDS
CAACTAGGTATGAGTTGCAA+CGG 0.621586 5_1:+6705513 Msa0714100:exon
ATAAACACTAGTTAGGAGAG+AGG 0.622037 5_1:-6705355 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 5_1:+6706101 Msa0714100:CDS
TGAAAGACATAAGGTCAGCA+CGG 0.634887 5_1:-6705653 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 5_1:-6705896 None:intergenic
GGTAAACGCAAGCGATCAGG+TGG 0.636761 5_1:+6706122 Msa0714100:CDS
GTTGAAACGGTGCTCCGTGG+CGG 0.644097 5_1:+6705957 Msa0714100:CDS
AAACTGGCACGTGTGTCAAG+CGG 0.645348 5_1:+6705932 Msa0714100:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 5_1:+6706205 Msa0714100:CDS
TGCTAAATACACCTCACATG+AGG 0.652353 5_1:-6705431 None:intergenic
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 5_1:+6705935 Msa0714100:CDS
TTCAGATGAGTCATCAACGG+TGG 0.656087 5_1:-6706027 None:intergenic
GCGGTTGAAACGGTGCTCCG+TGG 0.657002 5_1:+6705954 Msa0714100:CDS
ACTAGAGAACGAGAATGAGT+GGG 0.661667 5_1:-6705324 None:intergenic
GATTATTTACCAGGTCTCTG+TGG 0.665083 5_1:-6705693 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 5_1:+6705861 Msa0714100:CDS
GGTGTGTTACAAAATCTCAC+CGG 0.677482 5_1:-6706477 None:intergenic
GATTCCTCAGAATTCATCGA+CGG 0.685600 5_1:-6706248 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 5_1:-6705581 None:intergenic
AGAGAACGAGAATGAGTGGG+TGG 0.701859 5_1:-6705321 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 5_1:+6706244 Msa0714100:CDS
AAACTTGGCTACAAAGACAG+TGG 0.714204 5_1:-6705625 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_1:6706352-6706371 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - chr5_1:6706669-6706688 None:intergenic 15.0%
!! ATTAAAATTCTCAAACAAAA+AGG - chr5_1:6706352-6706371 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - chr5_1:6706669-6706688 None:intergenic 15.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_1:6705498-6705517 Msa0714100:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_1:6706572-6706591 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_1:6706643-6706662 None:intergenic 20.0%
!!! TTAGTAGTATTGAGAAATTT+TGG + chr5_1:6706730-6706749 Msa0714100:three_prime_UTR 20.0%
!!! TAGTAGTATTGAGAAATTTT+GGG + chr5_1:6706731-6706750 Msa0714100:three_prime_UTR 20.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + chr5_1:6705498-6705517 Msa0714100:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - chr5_1:6706572-6706591 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - chr5_1:6706643-6706662 None:intergenic 20.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_1:6705365-6705384 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_1:6705705-6705724 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_1:6705807-6705826 Msa0714100:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_1:6706382-6706401 Msa0714100:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_1:6706445-6706464 Msa0714100:three_prime_UTR 25.0%
! GGTATAAATAAACACTAGTT+AGG - chr5_1:6705365-6705384 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - chr5_1:6705705-6705724 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + chr5_1:6705807-6705826 Msa0714100:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + chr5_1:6706382-6706401 Msa0714100:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + chr5_1:6706445-6706464 Msa0714100:three_prime_UTR 25.0%
TATCGATCCATTGAATACAA+GGG - chr5_1:6705584-6705603 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_1:6705665-6705684 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_1:6706526-6706545 Msa0714100:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_1:6706527-6706546 Msa0714100:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + chr5_1:6706680-6706699 Msa0714100:three_prime_UTR 30.0%
TATCGATCCATTGAATACAA+GGG - chr5_1:6705584-6705603 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - chr5_1:6705665-6705684 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + chr5_1:6706526-6706545 Msa0714100:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + chr5_1:6706527-6706546 Msa0714100:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + chr5_1:6706680-6706699 Msa0714100:three_prime_UTR 30.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_1:6705358-6705377 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_1:6705403-6705422 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_1:6705574-6705593 Msa0714100:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_1:6705585-6705604 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_1:6705630-6705649 Msa0714100:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_1:6705741-6705760 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_1:6706337-6706356 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_1:6706504-6706523 None:intergenic 35.0%
ATAAACACTAGTTAGGAGAG+AGG - chr5_1:6705358-6705377 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - chr5_1:6705403-6705422 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + chr5_1:6705574-6705593 Msa0714100:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - chr5_1:6705585-6705604 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + chr5_1:6705630-6705649 Msa0714100:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - chr5_1:6705741-6705760 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - chr5_1:6706337-6706356 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - chr5_1:6706504-6706523 None:intergenic 35.0%
ACTAGAGAACGAGAATGAGT+GGG - chr5_1:6705327-6705346 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - chr5_1:6705328-6705347 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_1:6705404-6705423 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_1:6705411-6705430 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_1:6705434-6705453 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_1:6705513-6705532 Msa0714100:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_1:6705542-6705561 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_1:6705548-6705567 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_1:6705628-6705647 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_1:6705656-6705675 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_1:6705696-6705715 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_1:6705861-6705880 Msa0714100:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_1:6705985-6706004 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_1:6706033-6706052 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + chr5_1:6706142-6706161 Msa0714100:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_1:6706251-6706270 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_1:6706480-6706499 None:intergenic 40.0%
ACTAGAGAACGAGAATGAGT+GGG - chr5_1:6705327-6705346 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - chr5_1:6705328-6705347 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - chr5_1:6705404-6705423 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - chr5_1:6705411-6705430 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - chr5_1:6705434-6705453 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + chr5_1:6705513-6705532 Msa0714100:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - chr5_1:6705542-6705561 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - chr5_1:6705548-6705567 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - chr5_1:6705628-6705647 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - chr5_1:6705656-6705675 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - chr5_1:6705696-6705715 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + chr5_1:6705861-6705880 Msa0714100:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - chr5_1:6705985-6706004 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - chr5_1:6706033-6706052 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + chr5_1:6706142-6706161 Msa0714100:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - chr5_1:6706251-6706270 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - chr5_1:6706480-6706499 None:intergenic 40.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_1:6705352-6705371 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_1:6705387-6705406 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_1:6705388-6705407 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_1:6705515-6705534 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - chr5_1:6705693-6705712 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_1:6705916-6705935 Msa0714100:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_1:6705986-6706005 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_1:6705987-6706006 Msa0714100:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_1:6705993-6706012 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_1:6705994-6706013 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_1:6706030-6706049 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_1:6706029-6706048 Msa0714100:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_1:6706092-6706111 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_1:6706097-6706116 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_1:6706098-6706117 Msa0714100:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_1:6706106-6706125 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_1:6706164-6706183 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_1:6706165-6706184 Msa0714100:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_1:6706244-6706263 Msa0714100:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_1:6706259-6706278 Msa0714100:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_1:6706458-6706477 Msa0714100:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_1:6706485-6706504 Msa0714100:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_1:6706501-6706520 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_1:6706535-6706554 Msa0714100:three_prime_UTR 45.0%
ACTAGTTAGGAGAGAGGAAG+AGG - chr5_1:6705352-6705371 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - chr5_1:6705387-6705406 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - chr5_1:6705388-6705407 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - chr5_1:6705515-6705534 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - chr5_1:6705693-6705712 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + chr5_1:6705916-6705935 Msa0714100:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - chr5_1:6705986-6706005 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + chr5_1:6705987-6706006 Msa0714100:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - chr5_1:6705993-6706012 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - chr5_1:6705994-6706013 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - chr5_1:6706030-6706049 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + chr5_1:6706029-6706048 Msa0714100:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - chr5_1:6706092-6706111 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - chr5_1:6706097-6706116 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + chr5_1:6706098-6706117 Msa0714100:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - chr5_1:6706106-6706125 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - chr5_1:6706164-6706183 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + chr5_1:6706165-6706184 Msa0714100:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + chr5_1:6706244-6706263 Msa0714100:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + chr5_1:6706259-6706278 Msa0714100:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + chr5_1:6706458-6706477 Msa0714100:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + chr5_1:6706485-6706504 Msa0714100:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - chr5_1:6706501-6706520 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + chr5_1:6706535-6706554 Msa0714100:three_prime_UTR 45.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_1:6705306-6705325 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - chr5_1:6705316-6705335 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - chr5_1:6705324-6705343 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_1:6705386-6705405 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_1:6705420-6705439 Msa0714100:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_1:6705426-6705445 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_1:6705534-6705553 Msa0714100:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5_1:6705597-6705616 Msa0714100:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5_1:6705608-6705627 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_1:6705885-6705904 Msa0714100:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_1:6705901-6705920 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_1:6705932-6705951 Msa0714100:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_1:6705977-6705996 Msa0714100:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - chr5_1:6705999-6706018 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_1:6706085-6706104 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_1:6706084-6706103 Msa0714100:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_1:6706213-6706232 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_1:6706242-6706261 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_1:6706284-6706303 Msa0714100:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_1:6706497-6706516 Msa0714100:three_prime_UTR 50.0%
! GGTGGTAGTGAGGTATGGTT+AGG - chr5_1:6705306-6705325 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - chr5_1:6705316-6705335 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - chr5_1:6705324-6705343 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - chr5_1:6705386-6705405 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + chr5_1:6705420-6705439 Msa0714100:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - chr5_1:6705426-6705445 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + chr5_1:6705534-6705553 Msa0714100:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + chr5_1:6705597-6705616 Msa0714100:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - chr5_1:6705608-6705627 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + chr5_1:6705885-6705904 Msa0714100:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - chr5_1:6705901-6705920 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + chr5_1:6705932-6705951 Msa0714100:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + chr5_1:6705977-6705996 Msa0714100:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - chr5_1:6705999-6706018 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - chr5_1:6706085-6706104 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + chr5_1:6706084-6706103 Msa0714100:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - chr5_1:6706213-6706232 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - chr5_1:6706242-6706261 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + chr5_1:6706284-6706303 Msa0714100:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + chr5_1:6706497-6706516 Msa0714100:three_prime_UTR 50.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_1:6705311-6705330 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_1:6705533-6705552 Msa0714100:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5_1:6705607-6705626 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5_1:6705609-6705628 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_1:6705643-6705662 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - chr5_1:6705689-6705708 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - chr5_1:6705690-6705709 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_1:6705892-6705911 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_1:6705894-6705913 Msa0714100:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_1:6705900-6705919 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_1:6705904-6705923 Msa0714100:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_1:6705909-6705928 Msa0714100:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_1:6705944-6705963 Msa0714100:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_1:6705974-6705993 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_1:6706101-6706120 Msa0714100:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_1:6706119-6706138 Msa0714100:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + chr5_1:6706122-6706141 Msa0714100:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_1:6706187-6706206 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_1:6706194-6706213 Msa0714100:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_1:6706201-6706220 Msa0714100:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_1:6706205-6706224 Msa0714100:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_1:6706216-6706235 Msa0714100:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_1:6706278-6706297 Msa0714100:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_1:6706279-6706298 Msa0714100:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_1:6706287-6706306 Msa0714100:CDS 55.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - chr5_1:6705311-6705330 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + chr5_1:6705533-6705552 Msa0714100:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - chr5_1:6705607-6705626 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - chr5_1:6705609-6705628 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - chr5_1:6705643-6705662 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - chr5_1:6705689-6705708 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - chr5_1:6705690-6705709 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - chr5_1:6705892-6705911 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + chr5_1:6705894-6705913 Msa0714100:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - chr5_1:6705900-6705919 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + chr5_1:6705904-6705923 Msa0714100:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + chr5_1:6705909-6705928 Msa0714100:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + chr5_1:6705944-6705963 Msa0714100:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - chr5_1:6705974-6705993 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + chr5_1:6706101-6706120 Msa0714100:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + chr5_1:6706119-6706138 Msa0714100:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + chr5_1:6706122-6706141 Msa0714100:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - chr5_1:6706187-6706206 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + chr5_1:6706194-6706213 Msa0714100:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + chr5_1:6706201-6706220 Msa0714100:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + chr5_1:6706205-6706224 Msa0714100:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + chr5_1:6706216-6706235 Msa0714100:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + chr5_1:6706278-6706297 Msa0714100:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + chr5_1:6706279-6706298 Msa0714100:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + chr5_1:6706287-6706306 Msa0714100:CDS 55.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_1:6705899-6705918 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_1:6705957-6705976 Msa0714100:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_1:6706054-6706073 Msa0714100:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_1:6706197-6706216 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_1:6706204-6706223 Msa0714100:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_1:6706271-6706290 Msa0714100:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - chr5_1:6705899-6705918 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + chr5_1:6705957-6705976 Msa0714100:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + chr5_1:6706054-6706073 Msa0714100:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - chr5_1:6706197-6706216 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + chr5_1:6706204-6706223 Msa0714100:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + chr5_1:6706271-6706290 Msa0714100:CDS 60.0%
GTCCCTCCACCACAGAGACC+TGG + chr5_1:6705684-6705703 Msa0714100:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_1:6705935-6705954 Msa0714100:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_1:6705954-6705973 Msa0714100:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_1:6706238-6706257 None:intergenic 65.0%
GTCCCTCCACCACAGAGACC+TGG + chr5_1:6705684-6705703 Msa0714100:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + chr5_1:6705935-6705954 Msa0714100:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + chr5_1:6705954-6705973 Msa0714100:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - chr5_1:6706238-6706257 None:intergenic 65.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_1:6706223-6706242 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_1:6706220-6706239 Msa0714100:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_1:6706221-6706240 Msa0714100:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - chr5_1:6706223-6706242 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + chr5_1:6706220-6706239 Msa0714100:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + chr5_1:6706221-6706240 Msa0714100:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 6705296 6706752 6705296 ID=Msa0714100;Name=Msa0714100
chr5_1 mRNA 6705296 6706752 6705296 ID=Msa0714100-mRNA-1;Parent=Msa0714100;Name=Msa0714100-mRNA-1;_AED=0.05;_eAED=0.05;_QI=226|1|1|1|1|1|2|391|231
chr5_1 exon 6705296 6705705 6705296 ID=Msa0714100-mRNA-1:exon:2802;Parent=Msa0714100-mRNA-1
chr5_1 exon 6705850 6706752 6705850 ID=Msa0714100-mRNA-1:exon:2803;Parent=Msa0714100-mRNA-1
chr5_1 five_prime_UTR 6705296 6705521 6705296 ID=Msa0714100-mRNA-1:five_prime_utr;Parent=Msa0714100-mRNA-1
chr5_1 CDS 6705522 6705705 6705522 ID=Msa0714100-mRNA-1:cds;Parent=Msa0714100-mRNA-1
chr5_1 CDS 6705850 6706361 6705850 ID=Msa0714100-mRNA-1:cds;Parent=Msa0714100-mRNA-1
chr5_1 three_prime_UTR 6706362 6706752 6706362 ID=Msa0714100-mRNA-1:three_prime_utr;Parent=Msa0714100-mRNA-1
Gene Sequence

>Msa0714100

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGAAGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTCCACCACAGAGACCTGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCCGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTACACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGGTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGAAGACCGCAAAGTTCTCAACCTTTTTGTTTGA
Protein sequence

>Msa0714100

MSCNGCRVLRKGCSESCILRPCIQWIDTPEAQGHATVFVAKFFGRADLMSFISNVPPPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLKPLPEFLGLHALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGGEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDEDRKVLNLFV*