AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388170 MtrunA17_Chr5g0401401 95.671 231 10 0 1 231 1 231 1.15e-163 451
Msa1388170 MtrunA17_Chr4g0053911 69.710 241 55 6 1 231 1 233 2.44e-109 313
Msa1388170 MtrunA17_Chr1g0207471 56.579 152 61 3 1 147 1 152 5.76e-55 174
Msa1388170 MtrunA17_Chr7g0215281 64.545 110 39 0 1 110 3 112 2.49e-52 171
Msa1388170 MtrunA17_Chr6g0457431 61.818 110 42 0 1 110 3 112 2.35e-50 161
Msa1388170 MtrunA17_Chr6g0469271 46.154 169 80 5 1 164 3 165 2.22e-45 151
Msa1388170 MtrunA17_Chr6g0470651 48.413 126 63 2 1 124 3 128 2.33e-40 138
Msa1388170 MtrunA17_Chr6g0470631 52.294 109 50 2 1 107 3 111 1.75e-38 133
Msa1388170 MtrunA17_Chr1g0209841 50.000 112 38 2 1 107 3 101 1.47e-35 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388170 AT4G37540.1 55.645 248 85 8 1 231 1 240 9.53e-81 242
Msa1388170 AT3G49940.1 57.031 256 76 10 1 231 1 247 4.89e-80 240
Msa1388170 AT5G67420.2 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa1388170 AT5G67420.1 52.941 255 91 6 1 231 1 250 9.19e-75 227
Msa1388170 AT1G67100.1 65.455 110 38 0 1 110 3 112 6.52e-52 168
Msa1388170 AT3G02550.1 63.636 110 40 0 1 110 3 112 2.17e-49 162
Msa1388170 AT1G68510.1 57.025 121 50 1 1 119 3 123 4.41e-47 155

Find 102 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATGAACCGGACTTTC+TGG 0.160468 tig0021037:-2984 None:intergenic
GAAACTCCGGTAAGGGTTTA+AGG 0.160922 tig0021037:-2873 None:intergenic
TTAGTAGTATTGAGAAATTT+TGG 0.238378 tig0021037:+3620 Msa1388170:three_prime_UTR
ACGCGTTGACCAACGGTTTC+CGG 0.249914 tig0021037:+3091 Msa1388170:CDS
ATTAAAATTCTCAAACAAAA+AGG 0.262922 tig0021037:-3239 None:intergenic
GTGGCATGACCTTGAGCTTC+AGG 0.279564 tig0021037:-2496 None:intergenic
ACTCACTACAACCCTTTCTA+AGG 0.331551 tig0021037:-2435 None:intergenic
GAATTCATCGACGGTGTTCC+CGG 0.351208 tig0021037:-3129 None:intergenic
GGACTTTCTGGTTCGGATCT+CGG 0.355586 tig0021037:-2972 None:intergenic
AGTCCGACAGCACCGTTAAC+GGG 0.370602 tig0021037:-2787 None:intergenic
ACGCGTTCTGTGGCGTTAAC+CGG 0.381628 tig0021037:-3074 None:intergenic
CGGAACCCTTAAACCCTTAC+CGG 0.384595 tig0021037:+2867 Msa1388170:CDS
AAAGTCCGGTTCATGAGTTC+TGG 0.390533 tig0021037:+2988 Msa1388170:CDS
TATTACGCCCTTGTATTCAA+TGG 0.394479 tig0021037:+2464 Msa1388170:CDS
AACTAGGGTGCAAGTGCAAA+TGG 0.409490 tig0021037:-2275 None:intergenic
AAGTCCGACAGCACCGTTAA+CGG 0.413291 tig0021037:-2788 None:intergenic
GGTATAAATAAACACTAGTT+AGG 0.420813 tig0021037:-2252 None:intergenic
AAACTCCGGTAAGGGTTTAA+GGG 0.432686 tig0021037:-2872 None:intergenic
AAACCCTTACCGGAGTTTCT+CGG 0.442329 tig0021037:+2877 Msa1388170:CDS
CGGTTGCCGTGTCCTTAGAA+AGG 0.456637 tig0021037:+2423 Msa1388170:CDS
TTACCAGGTCTCTGTGGTGG+AGG 0.463406 tig0021037:-2577 None:intergenic
AGAAGAGGAAGAGAAGAGAA+TGG 0.464524 tig0021037:-2298 None:intergenic
GGTGGTAAACGCAAGCGATC+AGG 0.465080 tig0021037:+3009 Msa1388170:CDS
GAAGAGAAGAGAATGGAACT+AGG 0.469054 tig0021037:-2291 None:intergenic
ACTAGTTAGGAGAGAGGAAG+AGG 0.471446 tig0021037:-2239 None:intergenic
GCTTCATCGGCAATGAAATC+CGG 0.477949 tig0021037:+3348 Msa1388170:three_prime_UTR
TACCACCAGAACTCATGAAC+CGG 0.478203 tig0021037:-2993 None:intergenic
ATGAACCGGACTTTCTGGTT+CGG 0.483553 tig0021037:-2979 None:intergenic
AGCACCGTTAACGGGGTTCA+CGG 0.485215 tig0021037:-2779 None:intergenic
ACTAGGGTGCAAGTGCAAAT+GGG 0.485440 tig0021037:-2274 None:intergenic
TGTGACGACGGTGACGTGCT+TGG 0.492180 tig0021037:+3161 Msa1388170:CDS
TGGCATGACCTTGAGCTTCA+GGG 0.492713 tig0021037:-2495 None:intergenic
CGGAGGGAAATTCGCCGGCC+GGG 0.499369 tig0021037:+3111 Msa1388170:CDS
AGAACCGTGAACCCCGTTAA+CGG 0.510533 tig0021037:+2775 Msa1388170:CDS
CGTTGCAACTCATACCTAGT+TGG 0.511859 tig0021037:-2402 None:intergenic
GGTGGTAGTGAGGTATGGTT+AGG 0.513227 tig0021037:-2193 None:intergenic
CCGGAGGGAAATTCGCCGGC+CGG 0.514516 tig0021037:+3110 Msa1388170:CDS
GTGTCAAGCGGCGGTTGAAA+CGG 0.518728 tig0021037:+2834 Msa1388170:CDS
GGTTGCCGTGTCCTTAGAAA+GGG 0.520856 tig0021037:+2424 Msa1388170:CDS
CGTTAGAAATGAAAGACATA+AGG 0.526325 tig0021037:-2552 None:intergenic
GCGTGTAAACCGAGAAACTC+CGG 0.536983 tig0021037:-2886 None:intergenic
GAGATCCGAACCAGAAAGTC+CGG 0.539752 tig0021037:+2974 Msa1388170:CDS
TCATCGACGGTGTTCCCGGC+CGG 0.542141 tig0021037:-3125 None:intergenic
ACGGTGACGTGCTTGGAAAG+TGG 0.543942 tig0021037:+3168 Msa1388170:CDS
GTATCGATCCATTGAATACA+AGG 0.548656 tig0021037:-2472 None:intergenic
AAGAGAAGAGAATGGAACTA+GGG 0.549109 tig0021037:-2290 None:intergenic
CGGTGACGTGCTTGGAAAGT+GGG 0.551329 tig0021037:+3169 Msa1388170:CDS
AGAATGAGTGGGTGGTAGTG+AGG 0.560089 tig0021037:-2203 None:intergenic
TACCAGGTCTCTGTGGTGGA+GGG 0.561251 tig0021037:-2576 None:intergenic
TTCTGAGGAATCTGTGACGA+CGG 0.564218 tig0021037:+3149 Msa1388170:CDS
TGCTTGGAAAGTGGGATCGG+AGG 0.566383 tig0021037:+3177 Msa1388170:CDS
TAAACCGAGAAACTCCGGTA+AGG 0.574362 tig0021037:-2881 None:intergenic
CCGTTGGTCAACGCGTTCTG+TGG 0.575430 tig0021037:-3084 None:intergenic
ACACCTCACATGAGGAGAAG+AGG 0.577266 tig0021037:-2313 None:intergenic
CTTCCTCTTCTCCTCATGTG+AGG 0.577472 tig0021037:+2310 Msa1388170:five_prime_UTR
GTTTCCGGAGGGAAATTCGC+CGG 0.577846 tig0021037:+3106 Msa1388170:CDS
AACTAGAGAACGAGAATGAG+TGG 0.578906 tig0021037:-2215 None:intergenic
AATTTCCCTCCGGAAACCGT+TGG 0.583258 tig0021037:-3100 None:intergenic
AGAGAAAGATGAGGGTTCGT+CGG 0.584589 tig0021037:+3425 Msa1388170:three_prime_UTR
ACCGTTGATGACTCATCTGA+AGG 0.585962 tig0021037:+2919 Msa1388170:CDS
ACGTGCTTGGAAAGTGGGAT+CGG 0.586938 tig0021037:+3174 Msa1388170:CDS
CCGGCCGGCGAATTTCCCTC+CGG 0.587093 tig0021037:-3110 None:intergenic
AACCCCGTTAACGGTGCTGT+CGG 0.588780 tig0021037:+2784 Msa1388170:CDS
AAACCGAGAAACTCCGGTAA+GGG 0.589079 tig0021037:-2880 None:intergenic
TTAAACAGAGAGAAAGATGA+GGG 0.590185 tig0021037:+3417 Msa1388170:three_prime_UTR
GTCCCTCCACCACAGAGACC+TGG 0.592710 tig0021037:+2574 Msa1388170:CDS
AGATTAAGATCAGTCGTCGC+CGG 0.593377 tig0021037:-3051 None:intergenic
TATTTACCAGGTCTCTGTGG+TGG 0.593625 tig0021037:-2580 None:intergenic
GAGTGGGTGGTAGTGAGGTA+TGG 0.595919 tig0021037:-2198 None:intergenic
TCCTTCAGATGAGTCATCAA+CGG 0.596231 tig0021037:-2920 None:intergenic
CGACGACTGATCTTAATCTC+CGG 0.598907 tig0021037:+3055 Msa1388170:CDS
AAGTCACGTGCAACGACGCG+CGG 0.599047 tig0021037:+2944 Msa1388170:CDS
CGTTGACCAACGGTTTCCGG+AGG 0.601993 tig0021037:+3094 Msa1388170:CDS
TTTAAACAGAGAGAAAGATG+AGG 0.602638 tig0021037:+3416 Msa1388170:three_prime_UTR
CCACAGAACGCGTTGACCAA+CGG 0.603462 tig0021037:+3084 Msa1388170:CDS
CTAGGGTGCAAGTGCAAATG+GGG 0.606285 tig0021037:-2273 None:intergenic
AGGGTTTAAGGGTTCCGCCA+CGG 0.607346 tig0021037:-2861 None:intergenic
GGCATGACCTTGAGCTTCAG+GGG 0.607961 tig0021037:-2494 None:intergenic
TACAACCCTTTCTAAGGACA+CGG 0.609661 tig0021037:-2429 None:intergenic
ATCGATACCCCTGAAGCTCA+AGG 0.611816 tig0021037:+2487 Msa1388170:CDS
CAACTAGGTATGAGTTGCAA+CGG 0.621586 tig0021037:+2403 Msa1388170:exon
ATAAACACTAGTTAGGAGAG+AGG 0.622037 tig0021037:-2245 None:intergenic
GTCCGGTTCATGAGTTCTGG+TGG 0.622596 tig0021037:+2991 Msa1388170:CDS
TGAAAGACATAAGGTCAGCA+CGG 0.634887 tig0021037:-2543 None:intergenic
GTCCGACAGCACCGTTAACG+GGG 0.636214 tig0021037:-2786 None:intergenic
GGTAAACGCAAGCGATCAGG+TGG 0.636761 tig0021037:+3012 Msa1388170:CDS
GTTGAAACGGTGCTCCGTGG+CGG 0.644097 tig0021037:+2847 Msa1388170:CDS
AAACTGGCACGTGTGTCAAG+CGG 0.645348 tig0021037:+2822 Msa1388170:CDS
GTTGACCAACGGTTTCCGGA+GGG 0.646523 tig0021037:+3095 Msa1388170:CDS
TGCTAAATACACCTCACATG+AGG 0.652353 tig0021037:-2321 None:intergenic
CTGGCACGTGTGTCAAGCGG+CGG 0.654365 tig0021037:+2825 Msa1388170:CDS
TTCAGATGAGTCATCAACGG+TGG 0.656087 tig0021037:-2917 None:intergenic
GCGGTTGAAACGGTGCTCCG+TGG 0.657002 tig0021037:+2844 Msa1388170:CDS
ACTAGAGAACGAGAATGAGT+GGG 0.661667 tig0021037:-2214 None:intergenic
GATTATTTACCAGGTCTCTG+TGG 0.665083 tig0021037:-2583 None:intergenic
TCTTTGTTGTTTGAAGCGTG+TGG 0.669278 tig0021037:+2751 Msa1388170:CDS
GGTGTGTTACAAAATCTCAC+CGG 0.677482 tig0021037:-3367 None:intergenic
GATTCCTCAGAATTCATCGA+CGG 0.685600 tig0021037:-3138 None:intergenic
TATCGATCCATTGAATACAA+GGG 0.699979 tig0021037:-2471 None:intergenic
AGAGAACGAGAATGAGTGGG+TGG 0.701859 tig0021037:-2211 None:intergenic
AACACCGTCGATGAATTCTG+AGG 0.704407 tig0021037:+3134 Msa1388170:CDS
AAACTTGGCTACAAAGACAG+TGG 0.714204 tig0021037:-2515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTCTCAAACAAAA+AGG - tig0021037:3242-3261 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - tig0021037:3559-3578 None:intergenic 15.0%
!! ATTAAAATTCTCAAACAAAA+AGG - tig0021037:3242-3261 None:intergenic 15.0%
!! CAAAAACAACAATAAAATAA+CGG - tig0021037:3559-3578 None:intergenic 15.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + tig0021037:2388-2407 Msa1388170:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - tig0021037:3462-3481 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - tig0021037:3533-3552 None:intergenic 20.0%
!!! TTAGTAGTATTGAGAAATTT+TGG + tig0021037:3620-3639 Msa1388170:three_prime_UTR 20.0%
!!! TAGTAGTATTGAGAAATTTT+GGG + tig0021037:3621-3640 Msa1388170:three_prime_UTR 20.0%
!!! TTTAAGTTTTTTAACCAACT+AGG + tig0021037:2388-2407 Msa1388170:five_prime_UTR 20.0%
!! TGATTGATAGATCATAAAAA+AGG - tig0021037:3462-3481 None:intergenic 20.0%
!! AGAAAAATATGTTTCTTCTT+AGG - tig0021037:3533-3552 None:intergenic 20.0%
! GGTATAAATAAACACTAGTT+AGG - tig0021037:2255-2274 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - tig0021037:2595-2614 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + tig0021037:2697-2716 Msa1388170:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + tig0021037:3272-3291 Msa1388170:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + tig0021037:3335-3354 Msa1388170:three_prime_UTR 25.0%
! GGTATAAATAAACACTAGTT+AGG - tig0021037:2255-2274 None:intergenic 25.0%
! GAAGATAAAGATTATTTACC+AGG - tig0021037:2595-2614 None:intergenic 25.0%
! TAGATGAACTAAACTAACTA+AGG + tig0021037:2697-2716 Msa1388170:intron 25.0%
!!! AGATTCGTGTTTTTTGTTTA+TGG + tig0021037:3272-3291 Msa1388170:three_prime_UTR 25.0%
!!! TTTTTTTAGTGTTGCTTCAT+CGG + tig0021037:3335-3354 Msa1388170:three_prime_UTR 25.0%
TATCGATCCATTGAATACAA+GGG - tig0021037:2474-2493 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - tig0021037:2555-2574 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + tig0021037:3416-3435 Msa1388170:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + tig0021037:3417-3436 Msa1388170:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + tig0021037:3570-3589 Msa1388170:three_prime_UTR 30.0%
TATCGATCCATTGAATACAA+GGG - tig0021037:2474-2493 None:intergenic 30.0%
CGTTAGAAATGAAAGACATA+AGG - tig0021037:2555-2574 None:intergenic 30.0%
TTTAAACAGAGAGAAAGATG+AGG + tig0021037:3416-3435 Msa1388170:three_prime_UTR 30.0%
TTAAACAGAGAGAAAGATGA+GGG + tig0021037:3417-3436 Msa1388170:three_prime_UTR 30.0%
!!! TTGTTTTTGTTAGAGTCTAC+TGG + tig0021037:3570-3589 Msa1388170:three_prime_UTR 30.0%
ATAAACACTAGTTAGGAGAG+AGG - tig0021037:2248-2267 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - tig0021037:2293-2312 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + tig0021037:2464-2483 Msa1388170:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - tig0021037:2475-2494 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + tig0021037:2520-2539 Msa1388170:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - tig0021037:2631-2650 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - tig0021037:3227-3246 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - tig0021037:3394-3413 None:intergenic 35.0%
ATAAACACTAGTTAGGAGAG+AGG - tig0021037:2248-2267 None:intergenic 35.0%
AAGAGAAGAGAATGGAACTA+GGG - tig0021037:2293-2312 None:intergenic 35.0%
TATTACGCCCTTGTATTCAA+TGG + tig0021037:2464-2483 Msa1388170:CDS 35.0%
! GTATCGATCCATTGAATACA+AGG - tig0021037:2475-2494 None:intergenic 35.0%
! GTCTTTGTAGCCAAGTTTTT+CGG + tig0021037:2520-2539 Msa1388170:CDS 35.0%
! TTAGAGTGAAAGTGTAACTG+TGG - tig0021037:2631-2650 None:intergenic 35.0%
CAAAAAGGTTGAGAACTTTG+CGG - tig0021037:3227-3246 None:intergenic 35.0%
AAAACCAGAGAAAAATCCTG+TGG - tig0021037:3394-3413 None:intergenic 35.0%
ACTAGAGAACGAGAATGAGT+GGG - tig0021037:2217-2236 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - tig0021037:2218-2237 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - tig0021037:2294-2313 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - tig0021037:2301-2320 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - tig0021037:2324-2343 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + tig0021037:2403-2422 Msa1388170:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - tig0021037:2432-2451 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - tig0021037:2438-2457 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - tig0021037:2518-2537 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - tig0021037:2546-2565 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - tig0021037:2586-2605 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + tig0021037:2751-2770 Msa1388170:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - tig0021037:2875-2894 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - tig0021037:2923-2942 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + tig0021037:3032-3051 Msa1388170:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - tig0021037:3141-3160 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - tig0021037:3370-3389 None:intergenic 40.0%
ACTAGAGAACGAGAATGAGT+GGG - tig0021037:2217-2236 None:intergenic 40.0%
AACTAGAGAACGAGAATGAG+TGG - tig0021037:2218-2237 None:intergenic 40.0%
GAAGAGAAGAGAATGGAACT+AGG - tig0021037:2294-2313 None:intergenic 40.0%
AGAAGAGGAAGAGAAGAGAA+TGG - tig0021037:2301-2320 None:intergenic 40.0%
TGCTAAATACACCTCACATG+AGG - tig0021037:2324-2343 None:intergenic 40.0%
CAACTAGGTATGAGTTGCAA+CGG + tig0021037:2403-2422 Msa1388170:exon 40.0%
! TACAACCCTTTCTAAGGACA+CGG - tig0021037:2432-2451 None:intergenic 40.0%
ACTCACTACAACCCTTTCTA+AGG - tig0021037:2438-2457 None:intergenic 40.0%
AAACTTGGCTACAAAGACAG+TGG - tig0021037:2518-2537 None:intergenic 40.0%
TGAAAGACATAAGGTCAGCA+CGG - tig0021037:2546-2565 None:intergenic 40.0%
GATTATTTACCAGGTCTCTG+TGG - tig0021037:2586-2605 None:intergenic 40.0%
!! TCTTTGTTGTTTGAAGCGTG+TGG + tig0021037:2751-2770 Msa1388170:CDS 40.0%
AAACTCCGGTAAGGGTTTAA+GGG - tig0021037:2875-2894 None:intergenic 40.0%
TCCTTCAGATGAGTCATCAA+CGG - tig0021037:2923-2942 None:intergenic 40.0%
!!! TGGTGAAGTTTTGAAGCTTC+CGG + tig0021037:3032-3051 Msa1388170:CDS 40.0%
GATTCCTCAGAATTCATCGA+CGG - tig0021037:3141-3160 None:intergenic 40.0%
GGTGTGTTACAAAATCTCAC+CGG - tig0021037:3370-3389 None:intergenic 40.0%
ACTAGTTAGGAGAGAGGAAG+AGG - tig0021037:2242-2261 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - tig0021037:2277-2296 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - tig0021037:2278-2297 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - tig0021037:2405-2424 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - tig0021037:2583-2602 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + tig0021037:2806-2825 Msa1388170:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - tig0021037:2876-2895 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + tig0021037:2877-2896 Msa1388170:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - tig0021037:2883-2902 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - tig0021037:2884-2903 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - tig0021037:2920-2939 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + tig0021037:2919-2938 Msa1388170:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - tig0021037:2982-3001 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - tig0021037:2987-3006 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + tig0021037:2988-3007 Msa1388170:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - tig0021037:2996-3015 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - tig0021037:3054-3073 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + tig0021037:3055-3074 Msa1388170:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + tig0021037:3134-3153 Msa1388170:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + tig0021037:3149-3168 Msa1388170:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + tig0021037:3348-3367 Msa1388170:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + tig0021037:3375-3394 Msa1388170:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - tig0021037:3391-3410 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + tig0021037:3425-3444 Msa1388170:three_prime_UTR 45.0%
ACTAGTTAGGAGAGAGGAAG+AGG - tig0021037:2242-2261 None:intergenic 45.0%
ACTAGGGTGCAAGTGCAAAT+GGG - tig0021037:2277-2296 None:intergenic 45.0%
AACTAGGGTGCAAGTGCAAA+TGG - tig0021037:2278-2297 None:intergenic 45.0%
CGTTGCAACTCATACCTAGT+TGG - tig0021037:2405-2424 None:intergenic 45.0%
TATTTACCAGGTCTCTGTGG+TGG - tig0021037:2583-2602 None:intergenic 45.0%
! GACTTTTGTGGACAGGAAAC+TGG + tig0021037:2806-2825 Msa1388170:CDS 45.0%
GAAACTCCGGTAAGGGTTTA+AGG - tig0021037:2876-2895 None:intergenic 45.0%
AAACCCTTACCGGAGTTTCT+CGG + tig0021037:2877-2896 Msa1388170:CDS 45.0%
AAACCGAGAAACTCCGGTAA+GGG - tig0021037:2883-2902 None:intergenic 45.0%
TAAACCGAGAAACTCCGGTA+AGG - tig0021037:2884-2903 None:intergenic 45.0%
TTCAGATGAGTCATCAACGG+TGG - tig0021037:2920-2939 None:intergenic 45.0%
!! ACCGTTGATGACTCATCTGA+AGG + tig0021037:2919-2938 Msa1388170:CDS 45.0%
!! ATGAACCGGACTTTCTGGTT+CGG - tig0021037:2982-3001 None:intergenic 45.0%
AACTCATGAACCGGACTTTC+TGG - tig0021037:2987-3006 None:intergenic 45.0%
AAAGTCCGGTTCATGAGTTC+TGG + tig0021037:2988-3007 Msa1388170:CDS 45.0%
TACCACCAGAACTCATGAAC+CGG - tig0021037:2996-3015 None:intergenic 45.0%
AGATTAAGATCAGTCGTCGC+CGG - tig0021037:3054-3073 None:intergenic 45.0%
CGACGACTGATCTTAATCTC+CGG + tig0021037:3055-3074 Msa1388170:CDS 45.0%
AACACCGTCGATGAATTCTG+AGG + tig0021037:3134-3153 Msa1388170:CDS 45.0%
! TTCTGAGGAATCTGTGACGA+CGG + tig0021037:3149-3168 Msa1388170:CDS 45.0%
GCTTCATCGGCAATGAAATC+CGG + tig0021037:3348-3367 Msa1388170:three_prime_UTR 45.0%
! ATTTTGTAACACACCGCCAC+AGG + tig0021037:3375-3394 Msa1388170:three_prime_UTR 45.0%
ACCAGAGAAAAATCCTGTGG+CGG - tig0021037:3391-3410 None:intergenic 45.0%
AGAGAAAGATGAGGGTTCGT+CGG + tig0021037:3425-3444 Msa1388170:three_prime_UTR 45.0%
! GGTGGTAGTGAGGTATGGTT+AGG - tig0021037:2196-2215 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - tig0021037:2206-2225 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - tig0021037:2214-2233 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - tig0021037:2276-2295 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + tig0021037:2310-2329 Msa1388170:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - tig0021037:2316-2335 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + tig0021037:2424-2443 Msa1388170:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + tig0021037:2487-2506 Msa1388170:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - tig0021037:2498-2517 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + tig0021037:2775-2794 Msa1388170:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - tig0021037:2791-2810 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + tig0021037:2822-2841 Msa1388170:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + tig0021037:2867-2886 Msa1388170:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - tig0021037:2889-2908 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - tig0021037:2975-2994 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + tig0021037:2974-2993 Msa1388170:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - tig0021037:3103-3122 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - tig0021037:3132-3151 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + tig0021037:3174-3193 Msa1388170:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + tig0021037:3387-3406 Msa1388170:three_prime_UTR 50.0%
! GGTGGTAGTGAGGTATGGTT+AGG - tig0021037:2196-2215 None:intergenic 50.0%
! AGAATGAGTGGGTGGTAGTG+AGG - tig0021037:2206-2225 None:intergenic 50.0%
AGAGAACGAGAATGAGTGGG+TGG - tig0021037:2214-2233 None:intergenic 50.0%
CTAGGGTGCAAGTGCAAATG+GGG - tig0021037:2276-2295 None:intergenic 50.0%
CTTCCTCTTCTCCTCATGTG+AGG + tig0021037:2310-2329 Msa1388170:five_prime_UTR 50.0%
ACACCTCACATGAGGAGAAG+AGG - tig0021037:2316-2335 None:intergenic 50.0%
GGTTGCCGTGTCCTTAGAAA+GGG + tig0021037:2424-2443 Msa1388170:CDS 50.0%
ATCGATACCCCTGAAGCTCA+AGG + tig0021037:2487-2506 Msa1388170:CDS 50.0%
TGGCATGACCTTGAGCTTCA+GGG - tig0021037:2498-2517 None:intergenic 50.0%
AGAACCGTGAACCCCGTTAA+CGG + tig0021037:2775-2794 Msa1388170:CDS 50.0%
AAGTCCGACAGCACCGTTAA+CGG - tig0021037:2791-2810 None:intergenic 50.0%
AAACTGGCACGTGTGTCAAG+CGG + tig0021037:2822-2841 Msa1388170:CDS 50.0%
CGGAACCCTTAAACCCTTAC+CGG + tig0021037:2867-2886 Msa1388170:CDS 50.0%
GCGTGTAAACCGAGAAACTC+CGG - tig0021037:2889-2908 None:intergenic 50.0%
!! GGACTTTCTGGTTCGGATCT+CGG - tig0021037:2975-2994 None:intergenic 50.0%
GAGATCCGAACCAGAAAGTC+CGG + tig0021037:2974-2993 Msa1388170:CDS 50.0%
AATTTCCCTCCGGAAACCGT+TGG - tig0021037:3103-3122 None:intergenic 50.0%
GAATTCATCGACGGTGTTCC+CGG - tig0021037:3132-3151 None:intergenic 50.0%
ACGTGCTTGGAAAGTGGGAT+CGG + tig0021037:3174-3193 Msa1388170:CDS 50.0%
! ACCGCCACAGGATTTTTCTC+TGG + tig0021037:3387-3406 Msa1388170:three_prime_UTR 50.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - tig0021037:2201-2220 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + tig0021037:2423-2442 Msa1388170:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - tig0021037:2497-2516 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - tig0021037:2499-2518 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - tig0021037:2533-2552 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - tig0021037:2579-2598 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - tig0021037:2580-2599 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - tig0021037:2782-2801 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + tig0021037:2784-2803 Msa1388170:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - tig0021037:2790-2809 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + tig0021037:2794-2813 Msa1388170:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + tig0021037:2799-2818 Msa1388170:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + tig0021037:2834-2853 Msa1388170:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - tig0021037:2864-2883 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + tig0021037:2991-3010 Msa1388170:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + tig0021037:3009-3028 Msa1388170:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + tig0021037:3012-3031 Msa1388170:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - tig0021037:3077-3096 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + tig0021037:3084-3103 Msa1388170:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + tig0021037:3091-3110 Msa1388170:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + tig0021037:3095-3114 Msa1388170:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + tig0021037:3106-3125 Msa1388170:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + tig0021037:3168-3187 Msa1388170:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + tig0021037:3169-3188 Msa1388170:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + tig0021037:3177-3196 Msa1388170:CDS 55.0%
! GAGTGGGTGGTAGTGAGGTA+TGG - tig0021037:2201-2220 None:intergenic 55.0%
CGGTTGCCGTGTCCTTAGAA+AGG + tig0021037:2423-2442 Msa1388170:CDS 55.0%
GGCATGACCTTGAGCTTCAG+GGG - tig0021037:2497-2516 None:intergenic 55.0%
GTGGCATGACCTTGAGCTTC+AGG - tig0021037:2499-2518 None:intergenic 55.0%
GTCAGCACGGCCGAAAAACT+TGG - tig0021037:2533-2552 None:intergenic 55.0%
! TACCAGGTCTCTGTGGTGGA+GGG - tig0021037:2579-2598 None:intergenic 55.0%
TTACCAGGTCTCTGTGGTGG+AGG - tig0021037:2580-2599 None:intergenic 55.0%
AGCACCGTTAACGGGGTTCA+CGG - tig0021037:2782-2801 None:intergenic 55.0%
!! AACCCCGTTAACGGTGCTGT+CGG + tig0021037:2784-2803 Msa1388170:CDS 55.0%
AGTCCGACAGCACCGTTAAC+GGG - tig0021037:2790-2809 None:intergenic 55.0%
!!! ACGGTGCTGTCGGACTTTTG+TGG + tig0021037:2794-2813 Msa1388170:CDS 55.0%
! GCTGTCGGACTTTTGTGGAC+AGG + tig0021037:2799-2818 Msa1388170:CDS 55.0%
GTGTCAAGCGGCGGTTGAAA+CGG + tig0021037:2834-2853 Msa1388170:CDS 55.0%
AGGGTTTAAGGGTTCCGCCA+CGG - tig0021037:2864-2883 None:intergenic 55.0%
! GTCCGGTTCATGAGTTCTGG+TGG + tig0021037:2991-3010 Msa1388170:CDS 55.0%
GGTGGTAAACGCAAGCGATC+AGG + tig0021037:3009-3028 Msa1388170:CDS 55.0%
GGTAAACGCAAGCGATCAGG+TGG + tig0021037:3012-3031 Msa1388170:CDS 55.0%
ACGCGTTCTGTGGCGTTAAC+CGG - tig0021037:3077-3096 None:intergenic 55.0%
! CCACAGAACGCGTTGACCAA+CGG + tig0021037:3084-3103 Msa1388170:CDS 55.0%
! ACGCGTTGACCAACGGTTTC+CGG + tig0021037:3091-3110 Msa1388170:CDS 55.0%
GTTGACCAACGGTTTCCGGA+GGG + tig0021037:3095-3114 Msa1388170:CDS 55.0%
GTTTCCGGAGGGAAATTCGC+CGG + tig0021037:3106-3125 Msa1388170:CDS 55.0%
ACGGTGACGTGCTTGGAAAG+TGG + tig0021037:3168-3187 Msa1388170:CDS 55.0%
CGGTGACGTGCTTGGAAAGT+GGG + tig0021037:3169-3188 Msa1388170:CDS 55.0%
TGCTTGGAAAGTGGGATCGG+AGG + tig0021037:3177-3196 Msa1388170:CDS 55.0%
GTCCGACAGCACCGTTAACG+GGG - tig0021037:2789-2808 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + tig0021037:2847-2866 Msa1388170:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + tig0021037:2944-2963 Msa1388170:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - tig0021037:3087-3106 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + tig0021037:3094-3113 Msa1388170:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + tig0021037:3161-3180 Msa1388170:CDS 60.0%
GTCCGACAGCACCGTTAACG+GGG - tig0021037:2789-2808 None:intergenic 60.0%
GTTGAAACGGTGCTCCGTGG+CGG + tig0021037:2847-2866 Msa1388170:CDS 60.0%
AAGTCACGTGCAACGACGCG+CGG + tig0021037:2944-2963 Msa1388170:CDS 60.0%
CCGTTGGTCAACGCGTTCTG+TGG - tig0021037:3087-3106 None:intergenic 60.0%
CGTTGACCAACGGTTTCCGG+AGG + tig0021037:3094-3113 Msa1388170:CDS 60.0%
TGTGACGACGGTGACGTGCT+TGG + tig0021037:3161-3180 Msa1388170:CDS 60.0%
GTCCCTCCACCACAGAGACC+TGG + tig0021037:2574-2593 Msa1388170:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + tig0021037:2825-2844 Msa1388170:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + tig0021037:2844-2863 Msa1388170:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - tig0021037:3128-3147 None:intergenic 65.0%
GTCCCTCCACCACAGAGACC+TGG + tig0021037:2574-2593 Msa1388170:CDS 65.0%
CTGGCACGTGTGTCAAGCGG+CGG + tig0021037:2825-2844 Msa1388170:CDS 65.0%
GCGGTTGAAACGGTGCTCCG+TGG + tig0021037:2844-2863 Msa1388170:CDS 65.0%
TCATCGACGGTGTTCCCGGC+CGG - tig0021037:3128-3147 None:intergenic 65.0%
CCGGCCGGCGAATTTCCCTC+CGG - tig0021037:3113-3132 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + tig0021037:3110-3129 Msa1388170:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + tig0021037:3111-3130 Msa1388170:CDS 70.0%
CCGGCCGGCGAATTTCCCTC+CGG - tig0021037:3113-3132 None:intergenic 70.0%
CCGGAGGGAAATTCGCCGGC+CGG + tig0021037:3110-3129 Msa1388170:CDS 70.0%
CGGAGGGAAATTCGCCGGCC+GGG + tig0021037:3111-3130 Msa1388170:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0021037 gene 2186 3642 2186 ID=Msa1388170;Name=Msa1388170
tig0021037 mRNA 2186 3642 2186 ID=Msa1388170-mRNA-1;Parent=Msa1388170;Name=Msa1388170-mRNA-1;_AED=0.05;_eAED=0.05;_QI=226|1|1|1|1|1|2|391|231
tig0021037 exon 2186 2595 2186 ID=Msa1388170-mRNA-1:exon:11014;Parent=Msa1388170-mRNA-1
tig0021037 exon 2740 3642 2740 ID=Msa1388170-mRNA-1:exon:11015;Parent=Msa1388170-mRNA-1
tig0021037 five_prime_UTR 2186 2411 2186 ID=Msa1388170-mRNA-1:five_prime_utr;Parent=Msa1388170-mRNA-1
tig0021037 CDS 2412 2595 2412 ID=Msa1388170-mRNA-1:cds;Parent=Msa1388170-mRNA-1
tig0021037 CDS 2740 3251 2740 ID=Msa1388170-mRNA-1:cds;Parent=Msa1388170-mRNA-1
tig0021037 three_prime_UTR 3252 3642 3252 ID=Msa1388170-mRNA-1:three_prime_utr;Parent=Msa1388170-mRNA-1
Gene Sequence

>Msa1388170

ATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCATGTATATTACGCCCTTGTATTCAATGGATCGATACCCCTGAAGCTCAAGGTCATGCCACTGTCTTTGTAGCCAAGTTTTTCGGCCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTCCACCACAGAGACCTGCTTTGTTTCAATCTTTGTTGTTTGAAGCGTGTGGAAGAACCGTGAACCCCGTTAACGGTGCTGTCGGACTTTTGTGGACAGGAAACTGGCACGTGTGTCAAGCGGCGGTTGAAACGGTGCTCCGTGGCGGAACCCTTAAACCCTTACCGGAGTTTCTCGGTTTACACGCTCTTACTTCCACCGTTGATGACTCATCTGAAGGAGAAGTCACGTGCAACGACGCGCGGAAGATCCGAGATCCGAACCAGAAAGTCCGGTTCATGAGTTCTGGTGGTAAACGCAAGCGATCAGGTGGTGAAGTTTTGAAGCTTCCGGCGACGACTGATCTTAATCTCCGGTTAACGCCACAGAACGCGTTGACCAACGGTTTCCGGAGGGAAATTCGCCGGCCGGGAACACCGTCGATGAATTCTGAGGAATCTGTGACGACGGTGACGTGCTTGGAAAGTGGGATCGGAGGAGATAACTACGTTCACGACGAAGACCGCAAAGTTCTCAACCTTTTTGTTTGA
Protein sequence

>Msa1388170

MSCNGCRVLRKGCSESCILRPCIQWIDTPEAQGHATVFVAKFFGRADLMSFISNVPPPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLKPLPEFLGLHALTSTVDDSSEGEVTCNDARKIRDPNQKVRFMSSGGKRKRSGGEVLKLPATTDLNLRLTPQNALTNGFRREIRRPGTPSMNSEESVTTVTCLESGIGGDNYVHDEDRKVLNLFV*