AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0759030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759030 MtrunA17_Chr5g0405791 100.000 197 0 0 1 197 1 197 2.40e-147 407
Msa0759030 MtrunA17_Chr8g0371501 97.462 197 5 0 1 197 1 197 1.10e-143 397
Msa0759030 MtrunA17_Chr4g0037021 89.340 197 21 0 1 197 1 197 1.93e-133 372
Msa0759030 MtrunA17_Chr3g0117931 77.835 194 42 1 2 194 4 197 1.41e-116 330
Msa0759030 MtrunA17_Chr2g0325101 84.270 178 28 0 1 178 1 178 8.01e-116 327
Msa0759030 MtrunA17_Chr6g0486041 74.874 199 47 1 1 196 1 199 1.40e-111 317
Msa0759030 MtrunA17_Chr4g0046211 88.112 143 17 0 1 143 1 143 5.57e-94 270
Msa0759030 MtrunA17_Chr7g0265001 30.851 188 116 5 8 192 18 194 1.09e-25 98.6
Msa0759030 MtrunA17_Chr1g0182321 31.383 188 116 6 6 191 15 191 7.56e-25 96.7
Msa0759030 MtrunA17_Chr1g0182301 31.383 188 116 6 6 191 15 191 1.31e-24 95.9
Msa0759030 MtrunA17_Chr1g0212521 32.386 176 106 6 6 179 15 179 1.60e-24 95.9
Msa0759030 MtrunA17_Chr4g0030911 32.386 176 106 6 6 179 15 179 2.78e-24 95.1
Msa0759030 MtrunA17_Chr7g0263071 30.851 188 117 6 6 191 15 191 3.86e-24 94.7
Msa0759030 MtrunA17_Chr8g0384311 30.726 179 109 5 6 181 13 179 2.64e-23 92.4
Msa0759030 MtrunA17_Chr3g0130271 29.630 189 119 5 7 192 13 190 3.95e-23 92.0
Msa0759030 MtrunA17_Chr1g0199331 31.461 178 105 7 7 180 32 196 8.76e-23 90.9
Msa0759030 MtrunA17_Chr1g0180291 30.899 178 108 5 7 181 15 180 1.44e-22 90.5
Msa0759030 MtrunA17_Chr8g0349711 30.814 172 105 5 1 170 1 160 2.85e-22 89.7
Msa0759030 MtrunA17_Chr8g0335471 33.516 182 102 8 6 180 8 177 4.21e-22 89.0
Msa0759030 MtrunA17_Chr1g0159481 30.726 179 109 5 6 181 17 183 4.99e-22 89.4
Msa0759030 MtrunA17_Chr7g0275991 33.939 165 95 5 8 170 22 174 5.37e-22 89.4
Msa0759030 MtrunA17_Chr4g0055651 29.947 187 112 6 6 185 8 182 6.03e-22 88.6
Msa0759030 MtrunA17_Chr8g0390561 31.111 180 109 6 8 184 11 178 1.42e-21 87.4
Msa0759030 MtrunA17_Chr7g0250531 31.016 187 110 7 6 185 8 182 1.53e-21 87.4
Msa0759030 MtrunA17_Chr4g0055081 32.727 165 97 5 8 170 11 163 2.05e-21 87.4
Msa0759030 MtrunA17_Chr2g0276931 30.857 175 104 6 1 170 1 163 2.79e-21 87.0
Msa0759030 MtrunA17_Chr3g0109721 32.961 179 105 5 8 183 12 178 8.98e-21 85.5
Msa0759030 MtrunA17_Chr2g0319951 29.282 181 114 5 6 184 13 181 1.01e-20 85.9
Msa0759030 MtrunA17_Chr5g0400131 32.727 165 97 5 8 170 11 163 1.97e-20 84.7
Msa0759030 MtrunA17_Chr2g0327911 29.891 184 114 6 6 185 15 187 2.55e-20 84.7
Msa0759030 MtrunA17_Chr8g0346421 38.655 119 70 3 8 124 28 145 2.68e-20 85.1
Msa0759030 MtrunA17_Chr6g0458081 27.624 181 117 5 1 179 1 169 2.72e-20 84.3
Msa0759030 MtrunA17_Chr2g0290891 27.907 172 110 5 1 170 1 160 3.85e-20 84.0
Msa0759030 MtrunA17_Chr8g0349681 30.233 172 106 5 1 170 8 167 8.83e-20 86.3
Msa0759030 MtrunA17_Chr8g0349681 29.651 172 107 5 1 170 379 538 5.21e-19 84.3
Msa0759030 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 170 329 1.22e-18 83.2
Msa0759030 MtrunA17_Chr5g0433561 31.515 165 99 4 8 170 12 164 2.27e-19 81.6
Msa0759030 MtrunA17_Chr4g0040781 35.606 132 82 3 6 135 13 143 3.82e-19 81.6
Msa0759030 MtrunA17_Chr1g0201121 30.729 192 116 7 1 184 1 183 4.18e-19 81.3
Msa0759030 MtrunA17_Chr3g0123971 30.909 165 100 4 8 170 11 163 4.46e-19 81.3
Msa0759030 MtrunA17_Chr4g0046261 29.834 181 113 5 6 184 13 181 4.61e-19 81.3
Msa0759030 MtrunA17_Chr2g0276941 30.994 171 104 5 6 174 15 173 4.87e-19 81.3
Msa0759030 MtrunA17_Chr3g0112681 29.834 181 113 5 6 184 12 180 5.46e-19 81.3
Msa0759030 MtrunA17_Chr2g0315551 35.833 120 74 3 6 123 13 131 5.74e-19 81.3
Msa0759030 MtrunA17_Chr1g0174281 32.530 166 98 5 7 170 16 169 6.21e-19 81.3
Msa0759030 MtrunA17_Chr8g0382221 31.515 165 99 5 8 170 11 163 6.56e-19 80.9
Msa0759030 MtrunA17_Chr2g0283131 35.833 120 74 3 6 123 13 131 1.05e-18 80.5
Msa0759030 MtrunA17_Chr1g0197331 28.804 184 116 5 7 186 16 188 1.31e-18 80.1
Msa0759030 MtrunA17_Chr7g0234201 28.947 190 121 5 6 193 13 190 1.69e-18 79.7
Msa0759030 MtrunA17_Chr7g0231131 31.928 166 99 6 7 170 16 169 2.65e-18 79.3
Msa0759030 MtrunA17_Chr8g0381841 31.515 165 99 5 8 170 19 171 6.75e-18 78.6
Msa0759030 MtrunA17_Chr8g0392931 35.294 119 74 3 6 122 14 131 1.19e-17 77.8
Msa0759030 MtrunA17_Chr2g0331691 29.032 186 117 6 6 185 10 186 1.38e-17 77.4
Msa0759030 MtrunA17_Chr7g0214241 28.947 190 121 5 6 193 15 192 1.53e-17 77.4
Msa0759030 MtrunA17_Chr4g0034871 27.957 186 119 5 6 185 8 184 2.64e-17 76.6
Msa0759030 MtrunA17_Chr3g0123821 31.325 166 98 6 8 170 18 170 3.04e-17 76.6
Msa0759030 MtrunA17_Chr6g0466301 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0759030 MtrunA17_Chr6g0465491 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0759030 MtrunA17_Chr4g0032201 29.341 167 104 5 6 170 12 166 5.92e-17 75.9
Msa0759030 MtrunA17_Chr1g0189361 30.114 176 105 6 6 177 14 175 6.15e-17 75.9
Msa0759030 MtrunA17_Chr8g0351861 31.138 167 101 6 6 170 16 170 6.27e-17 75.9
Msa0759030 MtrunA17_Chr2g0325091 36.667 120 73 3 6 123 11 129 1.74e-16 74.3
Msa0759030 MtrunA17_Chr4g0004661 28.497 193 121 7 1 185 57 240 2.52e-16 74.7
Msa0759030 MtrunA17_Chr8g0383261 28.235 170 109 3 6 170 12 173 2.58e-16 73.9
Msa0759030 MtrunA17_Chr6g0482721 33.065 124 80 3 6 127 12 134 9.61e-16 72.4
Msa0759030 MtrunA17_Chr7g0259771 28.877 187 99 4 7 180 9 174 4.67e-15 72.8
Msa0759030 MtrunA17_Chr3g0138121 28.485 165 103 4 7 170 14 164 6.94e-14 67.4
Msa0759030 MtrunA17_Chr3g0138101 28.485 165 103 4 7 170 14 164 7.16e-14 67.4
Msa0759030 MtrunA17_Chr3g0138111 28.485 165 103 4 7 170 14 164 8.00e-14 67.4
Msa0759030 MtrunA17_Chr1g0185591 27.604 192 95 6 7 180 13 178 8.38e-14 69.3
Msa0759030 MtrunA17_Chr1g0191811 28.571 175 115 4 7 180 17 182 8.40e-13 66.2
Msa0759030 MtrunA17_Chr4g0046221 70.270 37 11 0 142 178 10 46 2.23e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759030 AT2G17800.2 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0759030 AT2G17800.1 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0759030 AT3G51300.1 95.431 197 9 0 1 197 1 197 1.40e-127 357
Msa0759030 AT4G35950.1 95.939 197 8 0 1 197 1 197 1.42e-127 357
Msa0759030 AT4G35020.1 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0759030 AT4G35020.2 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0759030 AT4G35020.3 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0759030 AT1G75840.1 91.573 178 15 0 1 178 1 178 5.36e-122 343
Msa0759030 AT1G20090.1 90.341 176 17 0 3 178 2 177 3.09e-119 336
Msa0759030 AT5G45970.1 86.034 179 25 0 1 179 1 179 1.71e-117 332
Msa0759030 AT4G28950.1 75.500 200 43 2 1 196 1 198 2.63e-111 317
Msa0759030 AT5G62880.1 80.447 179 35 0 2 180 4 182 8.69e-111 315
Msa0759030 AT3G48040.1 80.447 179 35 0 2 180 4 182 8.96e-110 313
Msa0759030 AT2G44690.1 75.000 192 48 0 6 197 18 209 2.40e-106 304
Msa0759030 AT5G62880.2 79.070 172 32 1 2 173 4 171 5.27e-103 295
Msa0759030 AT3G48040.2 80.368 163 32 0 2 164 4 166 4.32e-100 287
Msa0759030 AT3G51290.2 95.122 164 8 0 34 197 635 798 1.77e-96 297
Msa0759030 AT4G35020.4 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0759030 AT4G35020.5 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0759030 AT3G46060.2 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0759030 AT3G46060.3 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0759030 AT3G46060.1 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0759030 AT3G54840.2 33.333 168 97 6 7 171 34 189 8.90e-24 93.6
Msa0759030 AT3G54840.1 32.143 168 99 5 7 171 34 189 9.53e-24 93.6
Msa0759030 AT3G54840.3 31.928 166 98 5 9 171 2 155 3.62e-23 91.3
Msa0759030 AT1G02130.1 32.544 169 100 6 6 172 8 164 8.47e-23 91.3
Msa0759030 AT3G53610.2 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0759030 AT3G53610.3 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0759030 AT3G53610.1 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0759030 AT5G45130.1 33.333 165 96 4 8 170 12 164 1.10e-22 90.9
Msa0759030 AT5G59840.1 31.737 167 101 5 6 170 15 170 1.43e-22 90.9
Msa0759030 AT4G19640.1 33.333 165 96 4 8 170 12 164 2.49e-22 90.1
Msa0759030 AT1G43890.3 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0759030 AT1G43890.1 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0759030 AT1G43890.2 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0759030 AT1G16920.1 32.418 182 109 6 6 185 13 182 2.99e-22 90.1
Msa0759030 AT5G45130.2 33.129 163 95 4 10 170 22 172 5.58e-22 89.4
Msa0759030 AT1G73640.1 32.984 191 110 6 6 190 13 191 1.97e-21 88.6
Msa0759030 AT2G44610.1 33.333 165 96 5 8 170 11 163 2.27e-21 87.8
Msa0759030 AT5G03530.2 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0759030 AT5G03530.1 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0759030 AT4G18430.1 32.597 181 108 5 6 184 13 181 4.12e-21 87.0
Msa0759030 AT4G35860.1 27.624 181 117 5 1 179 1 169 1.11e-20 85.9
Msa0759030 AT4G17530.1 31.016 187 110 7 6 185 8 182 1.79e-20 85.1
Msa0759030 AT4G39890.1 34.731 167 92 6 8 170 11 164 1.82e-20 85.5
Msa0759030 AT5G47200.1 31.319 182 106 7 6 180 8 177 3.03e-20 84.7
Msa0759030 AT3G11730.1 29.940 167 103 5 6 170 8 162 3.37e-20 84.3
Msa0759030 AT2G22290.1 33.333 165 96 5 8 170 11 163 3.58e-20 84.3
Msa0759030 AT1G06400.1 32.335 167 99 5 6 170 13 167 6.72e-20 84.0
Msa0759030 AT4G19640.2 36.885 122 74 3 8 127 12 132 8.89e-20 82.0
Msa0759030 AT4G18800.1 36.667 120 73 3 6 123 13 131 9.53e-20 83.6
Msa0759030 AT1G09630.1 31.492 181 110 5 6 184 12 180 9.58e-20 83.6
Msa0759030 AT3G15060.1 31.285 179 109 5 8 184 15 181 1.65e-19 82.8
Msa0759030 AT4G17170.1 27.326 172 111 5 1 170 1 160 2.17e-19 82.4
Msa0759030 AT4G19640.3 34.074 135 86 3 8 140 12 145 2.68e-19 82.0
Msa0759030 AT2G31680.1 29.670 182 114 4 6 185 12 181 2.75e-19 82.4
Msa0759030 AT3G09900.1 32.934 167 99 6 6 170 15 170 3.29e-19 82.0
Msa0759030 AT3G07410.1 30.526 190 118 5 6 193 12 189 3.29e-19 82.0
Msa0759030 AT4G17160.1 26.744 172 112 5 1 170 1 160 3.69e-19 81.6
Msa0759030 AT1G18200.1 30.387 181 112 6 6 184 13 181 5.73e-19 81.6
Msa0759030 AT5G60860.1 30.387 181 112 5 6 184 13 181 5.85e-19 81.6
Msa0759030 AT2G43130.1 29.121 182 115 5 6 185 12 181 5.89e-19 81.3
Msa0759030 AT3G12160.1 32.530 166 98 5 7 170 16 169 5.91e-19 81.6
Msa0759030 AT5G45750.1 35.833 120 74 3 6 123 13 131 6.31e-19 81.3
Msa0759030 AT3G18820.1 29.570 186 116 6 6 185 8 184 7.28e-19 80.9
Msa0759030 AT5G03520.1 33.533 167 98 6 6 170 15 170 8.63e-19 80.9
Msa0759030 AT1G01200.1 34.132 167 95 6 7 170 29 183 1.66e-18 80.9
Msa0759030 AT1G28550.1 37.500 120 72 3 6 123 13 131 1.71e-18 80.1
Msa0759030 AT5G10260.1 32.121 165 98 5 8 170 11 163 2.11e-18 79.7
Msa0759030 AT1G05810.2 30.994 171 104 5 6 174 58 216 2.97e-18 80.5
Msa0759030 AT1G05810.1 29.670 182 114 5 6 185 12 181 3.26e-18 79.7
Msa0759030 AT2G33870.1 37.815 119 71 3 6 122 13 130 5.00e-18 79.0
Msa0759030 AT5G64990.1 33.333 165 96 5 8 170 9 161 7.57e-18 78.2
Msa0759030 AT5G47520.1 28.652 178 113 5 2 177 10 175 1.85e-17 77.4
Msa0759030 AT1G22740.1 29.167 192 119 6 1 184 1 183 2.47e-17 77.0
Msa0759030 AT1G52280.1 30.726 179 107 7 1 171 1 170 5.25e-17 76.3
Msa0759030 AT2G21880.1 27.174 184 121 4 6 184 9 184 9.54e-17 75.5
Msa0759030 AT4G39990.1 32.787 122 79 3 7 126 18 138 1.10e-16 75.5
Msa0759030 AT1G07410.1 34.454 119 75 3 6 122 12 129 1.27e-16 75.1
Msa0759030 AT5G59150.1 29.240 171 107 6 6 174 12 170 1.57e-16 75.1
Msa0759030 AT3G16100.1 29.947 187 114 7 1 179 1 178 1.87e-16 74.7
Msa0759030 AT5G47960.1 33.051 118 76 3 7 122 16 132 2.16e-16 74.7
Msa0759030 AT5G65270.1 28.729 181 113 6 7 184 18 185 2.31e-16 74.7
Msa0759030 AT4G09720.1 30.409 171 104 6 6 170 8 169 5.43e-16 73.2
Msa0759030 AT3G09910.3 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0759030 AT3G09910.1 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0759030 AT3G09910.2 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0759030 AT5G27540.1 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0759030 AT5G27540.2 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0759030 AT3G46830.1 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0759030 AT3G46830.2 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0759030 AT1G49300.2 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0759030 AT1G49300.1 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0759030 AT5G64990.2 31.977 172 96 6 8 170 9 168 3.92e-15 71.2
Msa0759030 AT5G55080.1 30.769 117 79 2 7 122 14 129 6.81e-15 70.9
Msa0759030 AT3G09910.5 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0759030 AT3G09910.4 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0759030 AT4G09720.4 28.409 176 106 5 6 170 8 174 1.45e-14 69.7
Msa0759030 AT3G63150.1 30.286 175 112 5 7 180 15 180 2.34e-14 71.2
Msa0759030 AT5G20020.1 28.485 165 103 4 7 170 14 164 8.48e-14 67.8
Msa0759030 AT5G20010.1 28.485 165 103 4 7 170 14 164 9.29e-14 67.8
Msa0759030 AT5G55190.1 31.092 119 80 2 7 124 14 131 9.68e-14 67.4
Msa0759030 AT4G09720.3 27.473 182 106 5 6 170 8 180 1.17e-13 67.4
Msa0759030 AT5G03520.2 30.137 146 90 5 47 191 47 181 1.91e-12 63.9
Msa0759030 AT4G35860.2 25.373 134 87 4 47 179 2 123 1.00e-11 61.2

Find 79 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAGATCTGAGGCTATTT+TGG 0.133154 5_2:-14559587 Msa0759030:five_prime_UTR
CTTTGTTCCAACCAGAATTA+TGG 0.197915 5_2:+14558371 None:intergenic
AGAAAGAGAAAGAACCTATT+TGG 0.261379 5_2:+14559874 None:intergenic
AGTGCGAATGTGGTTGTTAA+TGG 0.319066 5_2:-14558824 Msa0759030:CDS
TGAAATCAATAATAATAATA+AGG 0.321196 5_2:-14560044 None:intergenic
TAATGGAAGTATTGTGAATC+TGG 0.328007 5_2:-14558807 Msa0759030:CDS
TCTTCTGCTTTGGTGGTTGA+AGG 0.339738 5_2:+14557451 None:intergenic
AAACGACAATTCTTGTCATT+TGG 0.368834 5_2:-14557108 None:intergenic
TTTGTTCCAACCAGAATTAT+GGG 0.374664 5_2:+14558372 None:intergenic
TGGTTGAAGGACAACTCTTA+TGG 0.386728 5_2:+14557464 None:intergenic
TTATGAAAATGTCTCCAAAA+AGG 0.394383 5_2:-14558590 Msa0759030:intron
AAAGAAGGGGAGGGGAATAC+AGG 0.394999 5_2:+14557350 None:intergenic
CACACTCTCAGCTTCCAAAT+AGG 0.400756 5_2:-14559888 Msa0759030:five_prime_UTR
TTCTTTGTTATTGAAGAAGT+TGG 0.406930 5_2:-14557319 Msa0759030:three_prime_UTR
TTGTATCGAGATAGACCTTC+GGG 0.409474 5_2:-14557814 Msa0759030:intron
TTAAGCCACCTTATGCTTCA+AGG 0.411941 5_2:+14557254 None:intergenic
ATCACCTGAGCTGTGGTAAT+GGG 0.421707 5_2:+14557753 None:intergenic
AACCGTGGGGAAAGTATTGC+TGG 0.429322 5_2:+14559468 None:intergenic
CAAAGAAAATGATGAAAAGA+AGG 0.438811 5_2:+14557335 None:intergenic
AAAGAAGAATAAAGCACAAA+AGG 0.449553 5_2:-14557427 Msa0759030:CDS
AATCACCTGAGCTGTGGTAA+TGG 0.454383 5_2:+14557752 None:intergenic
TAATTGCCTTCTTCTCTCAC+AGG 0.456105 5_2:-14559843 Msa0759030:five_prime_UTR
GAAGAAGAAAGTACTGGAGG+AGG 0.457780 5_2:-14559624 Msa0759030:five_prime_UTR
ACCTGGTGTCCCCATAATTC+TGG 0.467734 5_2:-14558382 Msa0759030:CDS
CTAGATCTCCTTGAAGCATA+AGG 0.474002 5_2:-14557262 Msa0759030:three_prime_UTR
AAGAGGATATACGAAATAGT+TGG 0.475651 5_2:-14559953 Msa0759030:five_prime_UTR
AAAGAAAATGATGAAAAGAA+GGG 0.477205 5_2:+14557336 None:intergenic
GGTGCATAATGCTTCAACTC+TGG 0.481713 5_2:+14558402 None:intergenic
AAGAAGAAAGTACTGGAGGA+GGG 0.483171 5_2:-14559623 Msa0759030:five_prime_UTR
GGAGGAGGGGAACTGTCCTC+AGG 0.484285 5_2:-14559609 Msa0759030:five_prime_UTR
AGTGAATTACAATATTGAAC+AGG 0.490190 5_2:+14557404 None:intergenic
CCTAACCCTGTGAGAGAAGA+AGG 0.494457 5_2:+14559837 None:intergenic
ATTCTGGTTGGAACAAAGCT+TGG 0.495346 5_2:-14558366 Msa0759030:intron
TTTGTATCGAGATAGACCTT+CGG 0.498207 5_2:-14557815 Msa0759030:intron
GTGGTAATGGGAACGGCACC+AGG 0.503169 5_2:+14557765 None:intergenic
TATTGTGAATCTGGGTTTGT+GGG 0.507703 5_2:-14558798 Msa0759030:CDS
AATGGAAGTATTGTGAATCT+GGG 0.518446 5_2:-14558806 Msa0759030:CDS
AATGATGAAAAGAAGGGGAG+GGG 0.524862 5_2:+14557342 None:intergenic
CTGGGTTTGTGGGATACTGC+TGG 0.538632 5_2:-14558788 Msa0759030:intron
GTATTGTGAATCTGGGTTTG+TGG 0.538694 5_2:-14558799 Msa0759030:CDS
CGTTCCCATTACCACAGCTC+AGG 0.544810 5_2:-14557757 Msa0759030:intron
TGAATGCAGTTCAAAATCAC+AGG 0.553565 5_2:-14557606 Msa0759030:CDS
AAATGTGTTACTGTTGGTGA+TGG 0.556572 5_2:-14559526 Msa0759030:CDS
ACTGTTGGTGATGGAGCTGT+TGG 0.558196 5_2:-14559517 Msa0759030:CDS
TTTATTAAATGTGTTACTGT+TGG 0.563795 5_2:-14559532 Msa0759030:CDS
CACCAGCAATACTTTCCCCA+CGG 0.567542 5_2:-14559470 Msa0759030:intron
GAACTGCATTCAATGTAAGC+TGG 0.572826 5_2:+14557617 None:intergenic
AAATGATGAAAAGAAGGGGA+GGG 0.575158 5_2:+14557341 None:intergenic
CAGTTCTGCATAGACCATCC+TGG 0.577967 5_2:-14557783 Msa0759030:CDS
AAGAAAATGATGAAAAGAAG+GGG 0.582810 5_2:+14557337 None:intergenic
AGAAGAAAGTACTGGAGGAG+GGG 0.583883 5_2:-14559622 Msa0759030:five_prime_UTR
GAAGAAGAAGAAAGTACTGG+AGG 0.584132 5_2:-14559627 Msa0759030:five_prime_UTR
GTTCAATATTGTAATTCACT+AGG 0.584735 5_2:-14557401 Msa0759030:exon
AATTGCCTTCTTCTCTCACA+GGG 0.587915 5_2:-14559842 Msa0759030:intron
AAAATGATGAAAAGAAGGGG+AGG 0.591003 5_2:+14557340 None:intergenic
TATTTGTGGAACAGGGCAAG+AGG 0.600984 5_2:-14558692 Msa0759030:intron
ACCAGAATTATGGGGACACC+AGG 0.601533 5_2:+14558381 None:intergenic
GTATTTATTGCAGAACGTGA+AGG 0.606998 5_2:-14557502 Msa0759030:intron
GGTGTCCCCATAATTCTGGT+TGG 0.615899 5_2:-14558378 Msa0759030:CDS
GAGTTGAAGCATTATGCACC+TGG 0.616427 5_2:-14558399 Msa0759030:CDS
TCGGCACCACGGTAACTCAA+AGG 0.619029 5_2:+14558652 None:intergenic
TATTAAAGGGAGAAGAGCTG+AGG 0.626553 5_2:-14557655 Msa0759030:intron
TTGTTCCAACCAGAATTATG+GGG 0.627746 5_2:+14558373 None:intergenic
CTGAGCTGTGGTAATGGGAA+CGG 0.629176 5_2:+14557758 None:intergenic
TGTCCTCAGGTGAAGATCTG+AGG 0.631328 5_2:-14559596 Msa0759030:five_prime_UTR
GAAAGCCAATATGAAAACAT+CGG 0.632883 5_2:+14558633 None:intergenic
TTAAGACCTTTGAGTTACCG+TGG 0.650534 5_2:-14558658 Msa0759030:CDS
AGAAGTTGGAGAGTCTGTGT+GGG 0.653335 5_2:-14557305 Msa0759030:three_prime_UTR
TGTGTTAACAACGAAACGAA+CGG 0.653824 5_2:-14559925 Msa0759030:five_prime_UTR
TGACAATTTCAGTGCGAATG+TGG 0.655180 5_2:-14558834 Msa0759030:CDS
AGAACTGCTTATCATCCCGA+AGG 0.656406 5_2:+14557799 None:intergenic
AAGAAGTTGGAGAGTCTGTG+TGG 0.657940 5_2:-14557306 Msa0759030:three_prime_UTR
TAATGGGAACGGCACCAGGA+TGG 0.658560 5_2:+14557769 None:intergenic
TTTATTGCAGAACGTGAAGG+CGG 0.659717 5_2:-14557499 Msa0759030:intron
GATCTCCTTGAAGCATAAGG+TGG 0.664504 5_2:-14557259 Msa0759030:three_prime_UTR
GGGGAATACAGGTAGAACAA+AGG 0.683373 5_2:+14557361 None:intergenic
ATGCAGTTCAAAATCACAGG+AGG 0.690232 5_2:-14557603 Msa0759030:intron
TAGCCTCAGATCTTCACCTG+AGG 0.705958 5_2:+14559593 None:intergenic
ATATGAAAACATCGGCACCA+CGG 0.709782 5_2:+14558641 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTCAAATTTTTATTTT+TGG - chr5_2:14557730-14557749 Msa0759030:intron 10.0%
!!! TTGTTATTTGATTTATATTT+TGG - chr5_2:14558380-14558399 Msa0759030:CDS 10.0%
!! TTAATACCATATATAAATAT+GGG + chr5_2:14558847-14558866 None:intergenic 10.0%
!! ATTAATACCATATATAAATA+TGG + chr5_2:14558848-14558867 None:intergenic 10.0%
!! AATTGAAGAAAAAAGAAAAA+GGG + chr5_2:14557707-14557726 None:intergenic 15.0%
!! AAATTGAAGAAAAAAGAAAA+AGG + chr5_2:14557708-14557727 None:intergenic 15.0%
!! TGTTAATTTAAAGATTGAAA+TGG - chr5_2:14557920-14557939 Msa0759030:intron 15.0%
!!! TTTGGAATATATATGTAATT+GGG - chr5_2:14557962-14557981 Msa0759030:intron 15.0%
!!! GTTTGTTGAATTTAAAATTT+AGG - chr5_2:14557989-14558008 Msa0759030:intron 15.0%
!!! TGTAAATTTCAAATTTGTTA+TGG - chr5_2:14558035-14558054 Msa0759030:intron 15.0%
!!! TTATTTGATTTATATTTTGG+TGG - chr5_2:14558383-14558402 Msa0759030:CDS 15.0%
!! AAATACATAAAAAAACACAA+GGG + chr5_2:14558604-14558623 None:intergenic 15.0%
!! AAAATACATAAAAAAACACA+AGG + chr5_2:14558605-14558624 None:intergenic 15.0%
!!! TTGTGTTTTTTTATGTATTT+TGG - chr5_2:14558604-14558623 Msa0759030:CDS 15.0%
!! TAATACCATATATAAATATG+GGG + chr5_2:14558846-14558865 None:intergenic 15.0%
!! ATTTATATATGGTATTAATC+AGG - chr5_2:14558849-14558868 Msa0759030:CDS 15.0%
!! TTTATATATGGTATTAATCA+GGG - chr5_2:14558850-14558869 Msa0759030:CDS 15.0%
!! GAAAATATTTAATACAAATC+TGG + chr5_2:14559186-14559205 None:intergenic 15.0%
!!! TTTATTAAATGTGTTACTGT+TGG - chr5_2:14557606-14557625 Msa0759030:CDS 20.0%
!! ATTGAAGAAAAAAGAAAAAG+GGG + chr5_2:14557706-14557725 None:intergenic 20.0%
!!! AATTTTTATTTTTGGGTATC+TGG - chr5_2:14557738-14557757 Msa0759030:intron 20.0%
!!! ATTTTTATTTTTGGGTATCT+GGG - chr5_2:14557739-14557758 Msa0759030:intron 20.0%
!!! AATTTTGATTTTTCAGTGTT+TGG - chr5_2:14557801-14557820 Msa0759030:CDS 20.0%
!!! ATTCTTGTAAATTTGAAATC+TGG - chr5_2:14557835-14557854 Msa0759030:intron 20.0%
!! CAAATCATAATTTACATACT+CGG + chr5_2:14557885-14557904 None:intergenic 20.0%
!!! TAAATTATGATTTGGAATGA+TGG - chr5_2:14557891-14557910 Msa0759030:intron 20.0%
!!! AAATTATGATTTGGAATGAT+GGG - chr5_2:14557892-14557911 Msa0759030:intron 20.0%
!!! GTTTGGAATATATATGTAAT+TGG - chr5_2:14557961-14557980 Msa0759030:intron 20.0%
!!! GTTTGATTTAAAAGTTGAAA+TGG - chr5_2:14558116-14558135 Msa0759030:intron 20.0%
!! AATATTAAGTCGAAAAAAAC+TGG + chr5_2:14559283-14559302 None:intergenic 20.0%
!! GATACAAATGACAAAATAAT+AGG + chr5_2:14559311-14559330 None:intergenic 20.0%
!! ATTAAATTAAATCTGACATG+TGG - chr5_2:14559456-14559475 Msa0759030:intron 20.0%
!! TTAAATTAAATCTGACATGT+GGG - chr5_2:14559457-14559476 Msa0759030:intron 20.0%
!! AAAGAAAATGATGAAAAGAA+GGG + chr5_2:14559805-14559824 None:intergenic 20.0%
! ATCACAACTTTGTTCAAAAA+AGG - chr5_2:14557144-14557163 Msa0759030:three_prime_UTR 25.0%
! AATTGAGAAAATGCAGAATT+GGG + chr5_2:14557361-14557380 None:intergenic 25.0%
! TAATTGAGAAAATGCAGAAT+TGG + chr5_2:14557362-14557381 None:intergenic 25.0%
!! AATTCTGCATTTTCTCAATT+AGG - chr5_2:14557361-14557380 Msa0759030:three_prime_UTR 25.0%
!! ATTCTGCATTTTCTCAATTA+GGG - chr5_2:14557362-14557381 Msa0759030:three_prime_UTR 25.0%
! CGAGTATGTAAATTATGATT+TGG - chr5_2:14557883-14557902 Msa0759030:intron 25.0%
!! ATGGATATAATGTGAGATTT+TGG - chr5_2:14558135-14558154 Msa0759030:intron 25.0%
! TGATTGTTGGAATTAAAAGT+TGG - chr5_2:14558173-14558192 Msa0759030:intron 25.0%
! GATTGTTGGAATTAAAAGTT+GGG - chr5_2:14558174-14558193 Msa0759030:intron 25.0%
!!! ATATTGATTTGTGAATTTGC+TGG - chr5_2:14558217-14558236 Msa0759030:intron 25.0%
!!! ATTTTGTTGAACTTGCTTTT+TGG - chr5_2:14558251-14558270 Msa0759030:intron 25.0%
!!! TTTTGGTGGATTTTGTTATT+CGG - chr5_2:14558397-14558416 Msa0759030:CDS 25.0%
! GGATTTATTGTGTTTATTTG+TGG - chr5_2:14558432-14558451 Msa0759030:intron 25.0%
! TTATGAAAATGTCTCCAAAA+AGG - chr5_2:14558548-14558567 Msa0759030:intron 25.0%
!!! TATGTATTTTGGTTTATCTG+CGG - chr5_2:14558615-14558634 Msa0759030:CDS 25.0%
! ATATATAAATATGGGGAAGA+TGG + chr5_2:14558839-14558858 None:intergenic 25.0%
! ATCTTCCCCATATTTATATA+TGG - chr5_2:14558838-14558857 Msa0759030:CDS 25.0%
! TAGTTGATTATGAAATGCAT+GGG + chr5_2:14558902-14558921 None:intergenic 25.0%
! ATAGTTGATTATGAAATGCA+TGG + chr5_2:14558903-14558922 None:intergenic 25.0%
! AAAACTAAAACAAACCAGAA+AGG + chr5_2:14559125-14559144 None:intergenic 25.0%
!!! ATTTTTTATTCTGCTACTCT+AGG - chr5_2:14559406-14559425 Msa0759030:intron 25.0%
! ATTCTTCTACTAATTCACAA+TGG - chr5_2:14559581-14559600 Msa0759030:five_prime_UTR 25.0%
!! AATGTGAAGTTTAAGTTTGT+TGG - chr5_2:14559611-14559630 Msa0759030:five_prime_UTR 25.0%
! AAAGAAGAATAAAGCACAAA+AGG - chr5_2:14559711-14559730 Msa0759030:intron 25.0%
! AGTGAATTACAATATTGAAC+AGG + chr5_2:14559737-14559756 None:intergenic 25.0%
! GTTCAATATTGTAATTCACT+AGG - chr5_2:14559737-14559756 Msa0759030:intron 25.0%
! AAGAAAATGATGAAAAGAAG+GGG + chr5_2:14559804-14559823 None:intergenic 25.0%
! CAAAGAAAATGATGAAAAGA+AGG + chr5_2:14559806-14559825 None:intergenic 25.0%
!!! TTCTTTGTTATTGAAGAAGT+TGG - chr5_2:14559819-14559838 Msa0759030:intron 25.0%
AAGAGGATATACGAAATAGT+TGG - chr5_2:14557185-14557204 Msa0759030:three_prime_UTR 30.0%
AGAAAGAGAAAGAACCTATT+TGG + chr5_2:14557267-14557286 None:intergenic 30.0%
GAAATTTGGGGAAAAAAAGA+AGG + chr5_2:14557339-14557358 None:intergenic 30.0%
AATGCAGAATTGGGAAATTT+GGG + chr5_2:14557352-14557371 None:intergenic 30.0%
AAATGCAGAATTGGGAAATT+TGG + chr5_2:14557353-14557372 None:intergenic 30.0%
AATTCAAAGTTGAAAAAGCC+AGG + chr5_2:14557778-14557797 None:intergenic 30.0%
! TGTAAATTTGAAATCTGGCA+TGG - chr5_2:14557840-14557859 Msa0759030:intron 30.0%
AGATTGAAATGGATACAATG+TGG - chr5_2:14557931-14557950 Msa0759030:intron 30.0%
GATTGAAATGGATACAATGT+GGG - chr5_2:14557932-14557951 Msa0759030:intron 30.0%
!! GTTATGGAAAGTACTTTGTA+AGG - chr5_2:14558051-14558070 Msa0759030:intron 30.0%
TCGAGTTTATGTTTGATTGT+TGG - chr5_2:14558160-14558179 Msa0759030:intron 30.0%
CTGAAATTGTCAAAAACTGT+CGG + chr5_2:14558296-14558315 None:intergenic 30.0%
!! TAATGGAAGTATTGTGAATC+TGG - chr5_2:14558331-14558350 Msa0759030:intron 30.0%
!! AATGGAAGTATTGTGAATCT+GGG - chr5_2:14558332-14558351 Msa0759030:intron 30.0%
!!! TTTGGTGGATTTTGTTATTC+GGG - chr5_2:14558398-14558417 Msa0759030:CDS 30.0%
ATTGTGTTTATTTGTGGAAC+AGG - chr5_2:14558438-14558457 Msa0759030:intron 30.0%
TTGTGTTTATTTGTGGAACA+GGG - chr5_2:14558439-14558458 Msa0759030:intron 30.0%
GAAAGCCAATATGAAAACAT+CGG + chr5_2:14558508-14558527 None:intergenic 30.0%
! TTTGGAGACATTTTCATAAC+TGG + chr5_2:14558547-14558566 None:intergenic 30.0%
TTTGTTCCAACCAGAATTAT+GGG + chr5_2:14558769-14558788 None:intergenic 30.0%
AGTTGATTATGAAATGCATG+GGG + chr5_2:14558901-14558920 None:intergenic 30.0%
AGATACCAGTAGAAAAACAA+TGG + chr5_2:14559066-14559085 None:intergenic 30.0%
CATACATTGTATCTGCAATT+GGG - chr5_2:14559148-14559167 Msa0759030:intron 30.0%
ATACATTGTATCTGCAATTG+GGG - chr5_2:14559149-14559168 Msa0759030:intron 30.0%
TTAATACAAATCTGGAGACA+GGG + chr5_2:14559178-14559197 None:intergenic 30.0%
TTTAATACAAATCTGGAGAC+AGG + chr5_2:14559179-14559198 None:intergenic 30.0%
! TTCTTCTTTTTCTTCTGCTT+TGG + chr5_2:14559700-14559719 None:intergenic 30.0%
TGTGTTAACAACGAAACGAA+CGG - chr5_2:14557213-14557232 Msa0759030:three_prime_UTR 35.0%
GGGAAAAAAAGAAGGAAATG+AGG + chr5_2:14557331-14557350 None:intergenic 35.0%
ATGCAGAATTGGGAAATTTG+GGG + chr5_2:14557351-14557370 None:intergenic 35.0%
! GAAGAAGAAGAAGAAAGTAC+TGG - chr5_2:14557508-14557527 Msa0759030:intron 35.0%
!! TGAAGATCTGAGGCTATTTT+GGG - chr5_2:14557552-14557571 Msa0759030:intron 35.0%
!! TTTTCAAGATGAGTGCTTCT+AGG - chr5_2:14557583-14557602 Msa0759030:intron 35.0%
! AAATGTGTTACTGTTGGTGA+TGG - chr5_2:14557612-14557631 Msa0759030:CDS 35.0%
!! TATGATTTGGAATGATGGGT+TGG - chr5_2:14557896-14557915 Msa0759030:intron 35.0%
! ATGGATACAATGTGGGATTT+TGG - chr5_2:14557939-14557958 Msa0759030:intron 35.0%
!!! TACAATGTGGGATTTTGGTT+TGG - chr5_2:14557944-14557963 Msa0759030:intron 35.0%
! AAAGTACTTTGTAAGGCAAC+TGG - chr5_2:14558058-14558077 Msa0759030:intron 35.0%
AAGTACTTTGTAAGGCAACT+GGG - chr5_2:14558059-14558078 Msa0759030:intron 35.0%
!!! TGTTGAACTTGCTTTTTGGT+TGG - chr5_2:14558255-14558274 Msa0759030:intron 35.0%
TATTGTGAATCTGGGTTTGT+GGG - chr5_2:14558340-14558359 Msa0759030:intron 35.0%
TCTGCGGTGAACAATATATA+CGG - chr5_2:14558631-14558650 Msa0759030:CDS 35.0%
TTGTTCCAACCAGAATTATG+GGG + chr5_2:14558768-14558787 None:intergenic 35.0%
CTTTGTTCCAACCAGAATTA+TGG + chr5_2:14558770-14558789 None:intergenic 35.0%
!! CATCACCATTGTTTTTCTAC+TGG - chr5_2:14559058-14559077 Msa0759030:intron 35.0%
! GGTATCTGATACATCAGTTT+TGG - chr5_2:14559079-14559098 Msa0759030:intron 35.0%
!! TTTTGATGTTTCTGCCTTTC+TGG - chr5_2:14559108-14559127 Msa0759030:intron 35.0%
CCAATTGCAGATACAATGTA+TGG + chr5_2:14559150-14559169 None:intergenic 35.0%
CCATACATTGTATCTGCAAT+TGG - chr5_2:14559147-14559166 Msa0759030:intron 35.0%
AGCTAACAAAACTACTCGAT+GGG + chr5_2:14559218-14559237 None:intergenic 35.0%
AAGCTAACAAAACTACTCGA+TGG + chr5_2:14559219-14559238 None:intergenic 35.0%
TTAGCTTCCAGTTAGACAAA+TGG - chr5_2:14559232-14559251 Msa0759030:intron 35.0%
AAAAAACTGGTACACTGATC+AGG + chr5_2:14559270-14559289 None:intergenic 35.0%
TTTGTATCGAGATAGACCTT+CGG - chr5_2:14559323-14559342 Msa0759030:intron 35.0%
ATAAAAAATCACCTGAGCTG+TGG + chr5_2:14559395-14559414 None:intergenic 35.0%
TGAATGCAGTTCAAAATCAC+AGG - chr5_2:14559532-14559551 Msa0759030:CDS 35.0%
! TCAAAATCACAGGAGGTATA+TGG - chr5_2:14559542-14559561 Msa0759030:CDS 35.0%
GTATTTATTGCAGAACGTGA+AGG - chr5_2:14559636-14559655 Msa0759030:five_prime_UTR 35.0%
! TTCTTTTTCTTCTGCTTTGG+TGG + chr5_2:14559697-14559716 None:intergenic 35.0%
AAATGATGAAAAGAAGGGGA+GGG + chr5_2:14559800-14559819 None:intergenic 35.0%
AAAATGATGAAAAGAAGGGG+AGG + chr5_2:14559801-14559820 None:intergenic 35.0%
TAATTGCCTTCTTCTCTCAC+AGG - chr5_2:14557295-14557314 Msa0759030:three_prime_UTR 40.0%
AATTGCCTTCTTCTCTCACA+GGG - chr5_2:14557296-14557315 Msa0759030:three_prime_UTR 40.0%
!! GAAGAAGAAGAAAGTACTGG+AGG - chr5_2:14557511-14557530 Msa0759030:intron 40.0%
!! AAGAAGAAAGTACTGGAGGA+GGG - chr5_2:14557515-14557534 Msa0759030:intron 40.0%
!! GTGAAGATCTGAGGCTATTT+TGG - chr5_2:14557551-14557570 Msa0759030:intron 40.0%
CTGGGTTGTGTTTAAATTCC+TGG - chr5_2:14557757-14557776 Msa0759030:intron 40.0%
GGCATGGAAAATGCTTTGTA+AGG - chr5_2:14557856-14557875 Msa0759030:intron 40.0%
!!! CTTGCTTTTTGGTTGGAAAC+AGG - chr5_2:14558262-14558281 Msa0759030:intron 40.0%
TGACAATTTCAGTGCGAATG+TGG - chr5_2:14558304-14558323 Msa0759030:intron 40.0%
AGTGCGAATGTGGTTGTTAA+TGG - chr5_2:14558314-14558333 Msa0759030:intron 40.0%
GTATTGTGAATCTGGGTTTG+TGG - chr5_2:14558339-14558358 Msa0759030:intron 40.0%
! TTAAGACCTTTGAGTTACCG+TGG - chr5_2:14558480-14558499 Msa0759030:intron 40.0%
ATATGAAAACATCGGCACCA+CGG + chr5_2:14558500-14558519 None:intergenic 40.0%
!!! TGGTGCCGATGTTTTCATAT+TGG - chr5_2:14558500-14558519 Msa0759030:intron 40.0%
!!! GGAACAAGAAAGCACCTTTT+TGG + chr5_2:14558565-14558584 None:intergenic 40.0%
! TTCTTGTTCCCGTTTTGCTT+TGG - chr5_2:14558575-14558594 Msa0759030:intron 40.0%
CTGCGGTGAACAATATATAC+GGG - chr5_2:14558632-14558651 Msa0759030:CDS 40.0%
! CGTGCTTGTTTCATTTTCAG+TGG - chr5_2:14558710-14558729 Msa0759030:intron 40.0%
! ATTCTGGTTGGAACAAAGCT+TGG - chr5_2:14558772-14558791 Msa0759030:intron 40.0%
GAAGTTTCCATTTGTCGAAC+TGG - chr5_2:14558945-14558964 Msa0759030:intron 40.0%
!! ACCGGTTCTTTTTGTTTGCT+AGG - chr5_2:14558979-14558998 Msa0759030:intron 40.0%
CCCTAGCAAACAAAAAGAAC+CGG + chr5_2:14558983-14559002 None:intergenic 40.0%
!! CCGGTTCTTTTTGTTTGCTA+GGG - chr5_2:14558980-14558999 Msa0759030:intron 40.0%
!! CGGTTCTTTTTGTTTGCTAG+GGG - chr5_2:14558981-14559000 Msa0759030:intron 40.0%
!! GGTTCTTTTTGTTTGCTAGG+GGG - chr5_2:14558982-14559001 Msa0759030:intron 40.0%
!! TTTTTGTTTGCTAGGGGGAA+AGG - chr5_2:14558987-14559006 Msa0759030:intron 40.0%
!! TTTTGTTTGCTAGGGGGAAA+GGG - chr5_2:14558988-14559007 Msa0759030:intron 40.0%
AGCACAACCATTTGTCTAAC+TGG + chr5_2:14559242-14559261 None:intergenic 40.0%
TTGTATCGAGATAGACCTTC+GGG - chr5_2:14559324-14559343 Msa0759030:intron 40.0%
TGACATGTGGGTCCTATTAA+AGG - chr5_2:14559469-14559488 Msa0759030:intron 40.0%
GACATGTGGGTCCTATTAAA+GGG - chr5_2:14559470-14559489 Msa0759030:intron 40.0%
TCAGCTCTTCTCCCTTTAAT+AGG + chr5_2:14559484-14559503 None:intergenic 40.0%
TATTAAAGGGAGAAGAGCTG+AGG - chr5_2:14559483-14559502 Msa0759030:CDS 40.0%
GAACTGCATTCAATGTAAGC+TGG + chr5_2:14559524-14559543 None:intergenic 40.0%
ATGCAGTTCAAAATCACAGG+AGG - chr5_2:14559535-14559554 Msa0759030:CDS 40.0%
TTTATTGCAGAACGTGAAGG+CGG - chr5_2:14559639-14559658 Msa0759030:five_prime_UTR 40.0%
TGGTTGAAGGACAACTCTTA+TGG + chr5_2:14559677-14559696 None:intergenic 40.0%
AATGATGAAAAGAAGGGGAG+GGG + chr5_2:14559799-14559818 None:intergenic 40.0%
CTAGATCTCCTTGAAGCATA+AGG - chr5_2:14559876-14559895 Msa0759030:five_prime_UTR 40.0%
TTAAGCCACCTTATGCTTCA+AGG + chr5_2:14559887-14559906 None:intergenic 40.0%
CACACTCTCAGCTTCCAAAT+AGG - chr5_2:14557250-14557269 Msa0759030:three_prime_UTR 45.0%
CTTCTTCTCTCACAGGGTTA+GGG - chr5_2:14557302-14557321 Msa0759030:three_prime_UTR 45.0%
!! GAAGAAGAAAGTACTGGAGG+AGG - chr5_2:14557514-14557533 Msa0759030:intron 45.0%
!! AGAAGAAAGTACTGGAGGAG+GGG - chr5_2:14557516-14557535 Msa0759030:intron 45.0%
!!! GGTGGATTTTGTTATTCGGG+AGG - chr5_2:14558401-14558420 Msa0759030:CDS 45.0%
GTTATTCGGGAGGTTCTTTC+TGG - chr5_2:14558411-14558430 Msa0759030:CDS 45.0%
TATTTGTGGAACAGGGCAAG+AGG - chr5_2:14558446-14558465 Msa0759030:intron 45.0%
AAGGGACACCAAAGCAAAAC+GGG + chr5_2:14558586-14558605 None:intergenic 45.0%
CAAGGGACACCAAAGCAAAA+CGG + chr5_2:14558587-14558606 None:intergenic 45.0%
GGTGCATAATGCTTCAACTC+TGG + chr5_2:14558739-14558758 None:intergenic 45.0%
! GAGTTGAAGCATTATGCACC+TGG - chr5_2:14558739-14558758 Msa0759030:intron 45.0%
CAGTTAGCCAGTTCGACAAA+TGG + chr5_2:14558955-14558974 None:intergenic 45.0%
! TTTGTTTGCTAGGGGGAAAG+GGG - chr5_2:14558989-14559008 Msa0759030:intron 45.0%
AGAACTGCTTATCATCCCGA+AGG + chr5_2:14559342-14559361 None:intergenic 45.0%
ATCACCTGAGCTGTGGTAAT+GGG + chr5_2:14559388-14559407 None:intergenic 45.0%
AATCACCTGAGCTGTGGTAA+TGG + chr5_2:14559389-14559408 None:intergenic 45.0%
! TCTTCTGCTTTGGTGGTTGA+AGG + chr5_2:14559690-14559709 None:intergenic 45.0%
GGGGAATACAGGTAGAACAA+AGG + chr5_2:14559780-14559799 None:intergenic 45.0%
AAGAAGTTGGAGAGTCTGTG+TGG - chr5_2:14559832-14559851 Msa0759030:intron 45.0%
AGAAGTTGGAGAGTCTGTGT+GGG - chr5_2:14559833-14559852 Msa0759030:intron 45.0%
GATCTCCTTGAAGCATAAGG+TGG - chr5_2:14559879-14559898 Msa0759030:five_prime_UTR 45.0%
!! AATAAAATAATAAAAATAAG+AGG - chr5_2:14557168-14557187 Msa0759030:three_prime_UTR 5.0%
!!! AATTTCAAATTTTTATTTTT+GGG - chr5_2:14557731-14557750 Msa0759030:intron 5.0%
!! ATAAAAACTTAAAAAAATAT+GGG + chr5_2:14559974-14559993 None:intergenic 5.0%
!! AATAAAAACTTAAAAAAATA+TGG + chr5_2:14559975-14559994 None:intergenic 5.0%
CCTAACCCTGTGAGAGAAGA+AGG + chr5_2:14557304-14557323 None:intergenic 50.0%
CCTTCTTCTCTCACAGGGTT+AGG - chr5_2:14557301-14557320 Msa0759030:three_prime_UTR 50.0%
TGTCCTCAGGTGAAGATCTG+AGG - chr5_2:14557542-14557561 Msa0759030:intron 50.0%
TAGCCTCAGATCTTCACCTG+AGG + chr5_2:14557548-14557567 None:intergenic 50.0%
!! ACTGTTGGTGATGGAGCTGT+TGG - chr5_2:14557621-14557640 Msa0759030:CDS 50.0%
CACCAGCAATACTTTCCCCA+CGG - chr5_2:14557668-14557687 Msa0759030:intron 50.0%
!! AACCGTGGGGAAAGTATTGC+TGG + chr5_2:14557673-14557692 None:intergenic 50.0%
GGGAAGAATGACAAACCGTG+GGG + chr5_2:14557686-14557705 None:intergenic 50.0%
GGGGAAGAATGACAAACCGT+GGG + chr5_2:14557687-14557706 None:intergenic 50.0%
AGGGGAAGAATGACAAACCG+TGG + chr5_2:14557688-14557707 None:intergenic 50.0%
ACCTGGTGTCCCCATAATTC+TGG - chr5_2:14558756-14558775 Msa0759030:intron 50.0%
ACCAGAATTATGGGGACACC+AGG + chr5_2:14558760-14558779 None:intergenic 50.0%
GGTGTCCCCATAATTCTGGT+TGG - chr5_2:14558760-14558779 Msa0759030:intron 50.0%
GAACTGGCTAACTGCTGAAC+CGG - chr5_2:14558961-14558980 Msa0759030:intron 50.0%
! TTGTTTGCTAGGGGGAAAGG+GGG - chr5_2:14558990-14559009 Msa0759030:intron 50.0%
CAGTTCTGCATAGACCATCC+TGG - chr5_2:14559355-14559374 Msa0759030:intron 50.0%
!! CTGAGCTGTGGTAATGGGAA+CGG + chr5_2:14559383-14559402 None:intergenic 50.0%
AAAGAAGGGGAGGGGAATAC+AGG + chr5_2:14559791-14559810 None:intergenic 50.0%
CTGGGTTTGTGGGATACTGC+TGG - chr5_2:14558350-14558369 Msa0759030:intron 55.0%
TCGGCACCACGGTAACTCAA+AGG + chr5_2:14558489-14558508 None:intergenic 55.0%
TAGGGGGAAAGGGGGATTTC+TGG - chr5_2:14558998-14559017 Msa0759030:intron 55.0%
TAATGGGAACGGCACCAGGA+TGG + chr5_2:14559372-14559391 None:intergenic 55.0%
CGTTCCCATTACCACAGCTC+AGG - chr5_2:14559381-14559400 Msa0759030:intron 55.0%
!! GTGGTAATGGGAACGGCACC+AGG + chr5_2:14559376-14559395 None:intergenic 60.0%
GGAGGAGGGGAACTGTCCTC+AGG - chr5_2:14557529-14557548 Msa0759030:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 14557109 14560051 14557109 ID=Msa0759030;Name=Msa0759030
chr5_2 mRNA 14557109 14560051 14557109 ID=Msa0759030-mRNA-1;Parent=Msa0759030;Name=Msa0759030-mRNA-1;_AED=0.11;_eAED=0.11;_QI=320|1|1|1|0.85|0.75|8|301|197
chr5_2 exon 14557109 14557511 14557109 ID=Msa0759030-mRNA-1:exon:6627;Parent=Msa0759030-mRNA-1
chr5_2 exon 14557604 14557669 14557604 ID=Msa0759030-mRNA-1:exon:6626;Parent=Msa0759030-mRNA-1
chr5_2 exon 14557758 14557822 14557758 ID=Msa0759030-mRNA-1:exon:6625;Parent=Msa0759030-mRNA-1
chr5_2 exon 14558367 14558430 14558367 ID=Msa0759030-mRNA-1:exon:6624;Parent=Msa0759030-mRNA-1
chr5_2 exon 14558591 14558700 14558591 ID=Msa0759030-mRNA-1:exon:6623;Parent=Msa0759030-mRNA-1
chr5_2 exon 14558789 14558876 14558789 ID=Msa0759030-mRNA-1:exon:6622;Parent=Msa0759030-mRNA-1
chr5_2 exon 14559471 14559680 14559471 ID=Msa0759030-mRNA-1:exon:6621;Parent=Msa0759030-mRNA-1
chr5_2 exon 14559843 14560051 14559843 ID=Msa0759030-mRNA-1:exon:6620;Parent=Msa0759030-mRNA-1
chr5_2 five_prime_UTR 14559843 14560051 14559843 ID=Msa0759030-mRNA-1:five_prime_utr;Parent=Msa0759030-mRNA-1
chr5_2 five_prime_UTR 14559570 14559680 14559570 ID=Msa0759030-mRNA-1:five_prime_utr;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14559471 14559569 14559471 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14558789 14558876 14558789 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14558591 14558700 14558591 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14558367 14558430 14558367 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14557758 14557822 14557758 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14557604 14557669 14557604 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 CDS 14557410 14557511 14557410 ID=Msa0759030-mRNA-1:cds;Parent=Msa0759030-mRNA-1
chr5_2 three_prime_UTR 14557109 14557409 14557109 ID=Msa0759030-mRNA-1:three_prime_utr;Parent=Msa0759030-mRNA-1
Gene Sequence

>Msa0759030

ATGAGTGCTTCTAGGTTTATTAAATGTGTTACTGTTGGTGATGGAGCTGTTGGCAAAACTTGTTTGTTGATTTCTTACACCAGCAATACTTTCCCCACGGATTATGTGCCGACAGTTTTTGACAATTTCAGTGCGAATGTGGTTGTTAATGGAAGTATTGTGAATCTGGGTTTGTGGGATACTGCTGGGCAAGAGGATTATAACAGATTAAGACCTTTGAGTTACCGTGGTGCCGATGTTTTCATATTGGCTTTCTCTCTCATAAGCAAAGCCAGTTATGAAAATGTCTCCAAAAAGTGGATTCCAGAGTTGAAGCATTATGCACCTGGTGTCCCCATAATTCTGGTTGGAACAAAGCTTGACCTTCGGGATGATAAGCAGTTCTGCATAGACCATCCTGGTGCCGTTCCCATTACCACAGCTCAGGGAGAAGAGCTGAGGAAGCTGATTAATGCACCAGCTTACATTGAATGCAGTTCAAAATCACAGGAGAACGTGAAGGCGGTGTTTGACGCAGCCATAAGAGTTGTCCTTCAACCACCAAAGCAGAAGAAAAAGAAGAATAAAGCACAAAAGGCCTGTTCAATATTGTAA
Protein sequence

>Msa0759030

MSASRFIKCVTVGDGAVGKTCLLISYTSNTFPTDYVPTVFDNFSANVVVNGSIVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIILVGTKLDLRDDKQFCIDHPGAVPITTAQGEELRKLINAPAYIECSSKSQENVKAVFDAAIRVVLQPPKQKKKKNKAQKACSIL*