Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042770 | Msa0800390 | 0.808007 | 3.826666e-50 | -8.615850e-47 |
| Msa0622630 | Msa0800390 | 0.810028 | 1.413130e-50 | -8.615850e-47 |
| Msa0697380 | Msa0800390 | 0.884417 | 1.952226e-71 | -8.615850e-47 |
| Msa0757030 | Msa0800390 | 0.887879 | 9.675467e-73 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAGACCGTTGATGTTTC+AGG | 0.230060 | 5_3:-13772534 | None:intergenic |
| GTATCTGATGTGGTTTAGTT+TGG | 0.276776 | 5_3:+13773650 | Msa0800390:intron |
| TATCTGATGTGGTTTAGTTT+GGG | 0.288954 | 5_3:+13773651 | Msa0800390:three_prime_UTR |
| GCAACTTCCAAAGTCATTGA+TGG | 0.359430 | 5_3:+13772509 | Msa0800390:CDS |
| TTGGAAGTTGCAATGATGTC+AGG | 0.443874 | 5_3:-13772497 | None:intergenic |
| TCAGGAACCATCAATGACTT+TGG | 0.450270 | 5_3:-13772516 | None:intergenic |
| TTTGCAGTTAATATCCTTGA+GGG | 0.481204 | 5_3:+13773203 | Msa0800390:intron |
| CAATTTCTAACTGATTCAAA+TGG | 0.497571 | 5_3:+13772594 | Msa0800390:CDS |
| ATCTGATGTGGTTTAGTTTG+GGG | 0.497992 | 5_3:+13773652 | Msa0800390:three_prime_UTR |
| GTGAATCTAGGCGAGGGAAG+TGG | 0.550537 | 5_3:-13773237 | None:intergenic |
| AACGTACAGTAGGTGAATCT+AGG | 0.553854 | 5_3:-13773249 | None:intergenic |
| TTCTTTGTTAGTATCTGATG+TGG | 0.569322 | 5_3:+13773640 | Msa0800390:intron |
| TGGTTGTCTCATCACCCTCA+AGG | 0.606403 | 5_3:-13773217 | None:intergenic |
| CAGTAGGTGAATCTAGGCGA+GGG | 0.620624 | 5_3:-13773243 | None:intergenic |
| CTAGAAGCAACAGTTCTGCA+AGG | 0.631238 | 5_3:+13773680 | Msa0800390:three_prime_UTR |
| GTTAGACATGATGAAAACAA+AGG | 0.640821 | 5_3:+13773711 | Msa0800390:three_prime_UTR |
| ACAGTAGGTGAATCTAGGCG+AGG | 0.644049 | 5_3:-13773244 | None:intergenic |
| GATGGTTCCTGAAACATCAA+CGG | 0.663379 | 5_3:+13772527 | Msa0800390:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAATTTATTTAAAATT+AGG | - | chr5_3:13773442-13773461 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | + | chr5_3:13773445-13773464 | Msa0800390:intron | 0.0% |
| !!! | TAAAAAATTTATTTAAAATT+AGG | - | chr5_3:13773442-13773461 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | + | chr5_3:13773445-13773464 | Msa0800390:intron | 0.0% |
| !! | TGGATTATTTAATTATGATT+TGG | + | chr5_3:13772644-13772663 | Msa0800390:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr5_3:13772868-13772887 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr5_3:13772918-13772937 | None:intergenic | 15.0% |
| !! | TGGATTATTTAATTATGATT+TGG | + | chr5_3:13772644-13772663 | Msa0800390:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr5_3:13772868-13772887 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr5_3:13772918-13772937 | None:intergenic | 15.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr5_3:13772919-13772938 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr5_3:13773075-13773094 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr5_3:13773076-13773095 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr5_3:13773078-13773097 | Msa0800390:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | - | chr5_3:13773563-13773582 | None:intergenic | 20.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr5_3:13772919-13772938 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr5_3:13773075-13773094 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr5_3:13773076-13773095 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr5_3:13773078-13773097 | Msa0800390:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | - | chr5_3:13773563-13773582 | None:intergenic | 20.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr5_3:13772594-13772613 | Msa0800390:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr5_3:13772619-13772638 | Msa0800390:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr5_3:13773284-13773303 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr5_3:13773395-13773414 | Msa0800390:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr5_3:13773497-13773516 | None:intergenic | 25.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr5_3:13772594-13772613 | Msa0800390:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr5_3:13772619-13772638 | Msa0800390:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr5_3:13773284-13773303 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr5_3:13773395-13773414 | Msa0800390:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr5_3:13773497-13773516 | None:intergenic | 25.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr5_3:13772855-13772874 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr5_3:13772910-13772929 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr5_3:13772959-13772978 | Msa0800390:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr5_3:13772972-13772991 | Msa0800390:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr5_3:13772973-13772992 | Msa0800390:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr5_3:13773027-13773046 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr5_3:13773112-13773131 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr5_3:13773202-13773221 | Msa0800390:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr5_3:13773203-13773222 | Msa0800390:intron | 30.0% | |
| GAAAGAAAAAAACGTACAGT+AGG | - | chr5_3:13773262-13773281 | None:intergenic | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr5_3:13773489-13773508 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr5_3:13773490-13773509 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr5_3:13773640-13773659 | Msa0800390:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr5_3:13773651-13773670 | Msa0800390:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr5_3:13773711-13773730 | Msa0800390:three_prime_UTR | 30.0% | |
| ! | ATGAAAACAAAGGCATGTAA+GGG | + | chr5_3:13773721-13773740 | Msa0800390:three_prime_UTR | 30.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr5_3:13772855-13772874 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr5_3:13772910-13772929 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr5_3:13772959-13772978 | Msa0800390:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr5_3:13772972-13772991 | Msa0800390:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr5_3:13772973-13772992 | Msa0800390:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr5_3:13773027-13773046 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr5_3:13773112-13773131 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr5_3:13773202-13773221 | Msa0800390:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr5_3:13773203-13773222 | Msa0800390:intron | 30.0% | |
| GAAAGAAAAAAACGTACAGT+AGG | - | chr5_3:13773262-13773281 | None:intergenic | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr5_3:13773489-13773508 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr5_3:13773490-13773509 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr5_3:13773640-13773659 | Msa0800390:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr5_3:13773651-13773670 | Msa0800390:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr5_3:13773711-13773730 | Msa0800390:three_prime_UTR | 30.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr5_3:13772551-13772570 | Msa0800390:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr5_3:13772624-13772643 | Msa0800390:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr5_3:13772695-13772714 | Msa0800390:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr5_3:13772696-13772715 | Msa0800390:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr5_3:13773098-13773117 | Msa0800390:intron | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr5_3:13773337-13773356 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr5_3:13773338-13773357 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr5_3:13773338-13773357 | Msa0800390:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr5_3:13773339-13773358 | Msa0800390:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr5_3:13773347-13773366 | Msa0800390:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr5_3:13773650-13773669 | Msa0800390:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr5_3:13773652-13773671 | Msa0800390:three_prime_UTR | 35.0% |
| ! | GATGAAAACAAAGGCATGTA+AGG | + | chr5_3:13773720-13773739 | Msa0800390:three_prime_UTR | 35.0% |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr5_3:13772551-13772570 | Msa0800390:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr5_3:13772624-13772643 | Msa0800390:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr5_3:13772695-13772714 | Msa0800390:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr5_3:13772696-13772715 | Msa0800390:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr5_3:13773098-13773117 | Msa0800390:intron | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr5_3:13773337-13773356 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr5_3:13773338-13773357 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr5_3:13773338-13773357 | Msa0800390:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr5_3:13773339-13773358 | Msa0800390:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr5_3:13773347-13773366 | Msa0800390:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr5_3:13773650-13773669 | Msa0800390:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr5_3:13773652-13773671 | Msa0800390:three_prime_UTR | 35.0% |
| TTGGAAGTTGCAATGATGTC+AGG | - | chr5_3:13772500-13772519 | None:intergenic | 40.0% | |
| GCAACTTCCAAAGTCATTGA+TGG | + | chr5_3:13772509-13772528 | Msa0800390:CDS | 40.0% | |
| TCAGGAACCATCAATGACTT+TGG | - | chr5_3:13772519-13772538 | None:intergenic | 40.0% | |
| GATGGTTCCTGAAACATCAA+CGG | + | chr5_3:13772527-13772546 | Msa0800390:CDS | 40.0% | |
| TCATAGACCGTTGATGTTTC+AGG | - | chr5_3:13772537-13772556 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | - | chr5_3:13773252-13773271 | None:intergenic | 40.0% | |
| TTGGAAGTTGCAATGATGTC+AGG | - | chr5_3:13772500-13772519 | None:intergenic | 40.0% | |
| GCAACTTCCAAAGTCATTGA+TGG | + | chr5_3:13772509-13772528 | Msa0800390:CDS | 40.0% | |
| TCAGGAACCATCAATGACTT+TGG | - | chr5_3:13772519-13772538 | None:intergenic | 40.0% | |
| GATGGTTCCTGAAACATCAA+CGG | + | chr5_3:13772527-13772546 | Msa0800390:CDS | 40.0% | |
| TCATAGACCGTTGATGTTTC+AGG | - | chr5_3:13772537-13772556 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | - | chr5_3:13773252-13773271 | None:intergenic | 40.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | + | chr5_3:13773680-13773699 | Msa0800390:three_prime_UTR | 45.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | + | chr5_3:13773680-13773699 | Msa0800390:three_prime_UTR | 45.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr5_3:13773220-13773239 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | - | chr5_3:13773246-13773265 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | - | chr5_3:13773247-13773266 | None:intergenic | 50.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr5_3:13773220-13773239 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | - | chr5_3:13773246-13773265 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | - | chr5_3:13773247-13773266 | None:intergenic | 50.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | - | chr5_3:13773240-13773259 | None:intergenic | 55.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | - | chr5_3:13773240-13773259 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 13772486 | 13773740 | 13772486 | ID=Msa0800390;Name=Msa0800390 |
| chr5_3 | mRNA | 13772486 | 13773740 | 13772486 | ID=Msa0800390-mRNA-1;Parent=Msa0800390;Name=Msa0800390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=6|1|1|1|0.5|0.33|3|101|55 |
| chr5_3 | exon | 13772486 | 13772615 | 13772486 | ID=Msa0800390-mRNA-1:exon:5704;Parent=Msa0800390-mRNA-1 |
| chr5_3 | exon | 13773210 | 13773264 | 13773210 | ID=Msa0800390-mRNA-1:exon:5705;Parent=Msa0800390-mRNA-1 |
| chr5_3 | exon | 13773651 | 13773740 | 13773651 | ID=Msa0800390-mRNA-1:exon:5706;Parent=Msa0800390-mRNA-1 |
| chr5_3 | five_prime_UTR | 13772486 | 13772491 | 13772486 | ID=Msa0800390-mRNA-1:five_prime_utr;Parent=Msa0800390-mRNA-1 |
| chr5_3 | CDS | 13772492 | 13772615 | 13772492 | ID=Msa0800390-mRNA-1:cds;Parent=Msa0800390-mRNA-1 |
| chr5_3 | CDS | 13773210 | 13773253 | 13773210 | ID=Msa0800390-mRNA-1:cds;Parent=Msa0800390-mRNA-1 |
| chr5_3 | three_prime_UTR | 13773254 | 13773264 | 13773254 | ID=Msa0800390-mRNA-1:three_prime_utr;Parent=Msa0800390-mRNA-1 |
| chr5_3 | three_prime_UTR | 13773651 | 13773740 | 13773651 | ID=Msa0800390-mRNA-1:three_prime_utr;Parent=Msa0800390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
