AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0042770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGCATACGGTCCTTTCT+TGG 0.323111 1_1:+73672263 Msa0042770:CDS
GGAAGTCTTGTTGATTTAGT+TGG 0.335009 1_1:+73672231 Msa0042770:CDS
TGGATGAGGTTACCTTTGAA+TGG 0.410546 1_1:+73672283 Msa0042770:CDS
TGGTATGGAACTTCTTTCTC+CGG 0.445848 1_1:-73672156 None:intergenic
GGATGAGGTTACCTTTGAAT+GGG 0.455125 1_1:+73672284 Msa0042770:CDS
ATTGCAAATGACAATCTTGA+AGG 0.456999 1_1:+73672210 Msa0042770:CDS
ATTGTGTGATTGATTCATCT+TGG 0.474013 1_1:-73672176 None:intergenic
AATATCTTTATCCCATTCAA+AGG 0.483583 1_1:-73672295 None:intergenic
ATACGGTCCTTTCTTGGATG+AGG 0.503815 1_1:+73672269 Msa0042770:CDS
GTGATTGATTCATCTTGGTA+TGG 0.505565 1_1:-73672171 None:intergenic
CTTAAAAGAATTATGTCTAA+AGG 0.506090 1_1:+73671090 None:intergenic
TGATTTAGTTGGAGAAGTTG+AGG 0.526516 1_1:+73672242 Msa0042770:CDS
AACTTTAGAAGATAGTAAGC+CGG 0.555137 1_1:+73672137 Msa0042770:CDS
AAGGTAACCTCATCCAAGAA+AGG 0.563434 1_1:-73672276 None:intergenic
GGAGAAGTTGAGGAAGCATA+CGG 0.617796 1_1:+73672252 Msa0042770:CDS
TGATGAAACACATTCTGACT+AGG 0.628460 1_1:+73672377 Msa0042770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATATGAAATTTAATAAAA+AGG - chr1_1:73671446-73671465 None:intergenic 10.0%
!! TGATATGAAATTTAATAAAA+AGG - chr1_1:73671446-73671465 None:intergenic 10.0%
!! AAGTAATTTGATATTACATA+GGG - chr1_1:73671527-73671546 None:intergenic 15.0%
!! AAAGTAATTTGATATTACAT+AGG - chr1_1:73671528-73671547 None:intergenic 15.0%
!! TTGTAAAAAAAGTAGTAAAT+AGG - chr1_1:73671665-73671684 None:intergenic 15.0%
!! AAATACATATCTTGATTAAT+GGG - chr1_1:73671715-73671734 None:intergenic 15.0%
!! AAAATACATATCTTGATTAA+TGG - chr1_1:73671716-73671735 None:intergenic 15.0%
!! AAAAACACATAAATTTATTG+TGG + chr1_1:73671820-73671839 Msa0042770:intron 15.0%
!! AAGTAATTTGATATTACATA+GGG - chr1_1:73671527-73671546 None:intergenic 15.0%
!! AAAGTAATTTGATATTACAT+AGG - chr1_1:73671528-73671547 None:intergenic 15.0%
!! TTGTAAAAAAAGTAGTAAAT+AGG - chr1_1:73671665-73671684 None:intergenic 15.0%
!! AAATACATATCTTGATTAAT+GGG - chr1_1:73671715-73671734 None:intergenic 15.0%
!! AAAATACATATCTTGATTAA+TGG - chr1_1:73671716-73671735 None:intergenic 15.0%
!! AAAAACACATAAATTTATTG+TGG + chr1_1:73671820-73671839 Msa0042770:intron 15.0%
!!! TGAATTGTATATTTGCATTT+TGG + chr1_1:73671272-73671291 Msa0042770:intron 20.0%
!!! TGAATTGTATATTTGCATTT+TGG + chr1_1:73671272-73671291 Msa0042770:intron 20.0%
! ATATGTAGTTTATAAGACTC+AGG - chr1_1:73671191-73671210 None:intergenic 25.0%
!!! TTGTATATTTGCATTTTGGT+TGG + chr1_1:73671276-73671295 Msa0042770:intron 25.0%
!! TTGTTAAGCCTTTTGTATAA+TGG + chr1_1:73671419-73671438 Msa0042770:intron 25.0%
! AAAAAGGACCATTATACAAA+AGG - chr1_1:73671430-73671449 None:intergenic 25.0%
!! AGTTAAACTAGCACATTTTT+CGG + chr1_1:73671577-73671596 Msa0042770:intron 25.0%
! ACAATAGTATGATCAAAACT+AGG - chr1_1:73671604-73671623 None:intergenic 25.0%
!!! TACTATTGTTTTATGAAAGC+TGG + chr1_1:73671615-73671634 Msa0042770:intron 25.0%
!! CTCATATTGATAATAGCAAA+AGG - chr1_1:73671796-73671815 None:intergenic 25.0%
! ATTGTTGAAGTTTACTATAG+TGG + chr1_1:73671907-73671926 Msa0042770:intron 25.0%
!! TTTTTACCTTGATTCAAAGT+GGG - chr1_1:73671956-73671975 None:intergenic 25.0%
!! CTTGTTGTGCAAATATAAAT+GGG - chr1_1:73672036-73672055 None:intergenic 25.0%
! AATATCTTTATCCCATTCAA+AGG - chr1_1:73672298-73672317 None:intergenic 25.0%
! ATATGTAGTTTATAAGACTC+AGG - chr1_1:73671191-73671210 None:intergenic 25.0%
!!! TTGTATATTTGCATTTTGGT+TGG + chr1_1:73671276-73671295 Msa0042770:intron 25.0%
!! TTGTTAAGCCTTTTGTATAA+TGG + chr1_1:73671419-73671438 Msa0042770:intron 25.0%
! AAAAAGGACCATTATACAAA+AGG - chr1_1:73671430-73671449 None:intergenic 25.0%
!! AGTTAAACTAGCACATTTTT+CGG + chr1_1:73671577-73671596 Msa0042770:intron 25.0%
! ACAATAGTATGATCAAAACT+AGG - chr1_1:73671604-73671623 None:intergenic 25.0%
!!! TACTATTGTTTTATGAAAGC+TGG + chr1_1:73671615-73671634 Msa0042770:intron 25.0%
!! CTCATATTGATAATAGCAAA+AGG - chr1_1:73671796-73671815 None:intergenic 25.0%
! ATTGTTGAAGTTTACTATAG+TGG + chr1_1:73671907-73671926 Msa0042770:intron 25.0%
!! TTTTTACCTTGATTCAAAGT+GGG - chr1_1:73671956-73671975 None:intergenic 25.0%
!! CTTGTTGTGCAAATATAAAT+GGG - chr1_1:73672036-73672055 None:intergenic 25.0%
! AATATCTTTATCCCATTCAA+AGG - chr1_1:73672298-73672317 None:intergenic 25.0%
CCTTTAACATACGAAGATAA+TGG - chr1_1:73671122-73671141 None:intergenic 30.0%
CCATTATCTTCGTATGTTAA+AGG + chr1_1:73671119-73671138 Msa0042770:intron 30.0%
TACCGAGCTATTAGAAAAAA+GGG + chr1_1:73671389-73671408 Msa0042770:intron 30.0%
!!! TTCCCTTTTTTCTAATAGCT+CGG - chr1_1:73671394-73671413 None:intergenic 30.0%
AAGAAAAGAATTATGTGGTG+TGG - chr1_1:73671504-73671523 None:intergenic 30.0%
TAGGGAAGAAAAGAATTATG+TGG - chr1_1:73671509-73671528 None:intergenic 30.0%
! TTGAAGTTTACTATAGTGGA+TGG + chr1_1:73671911-73671930 Msa0042770:intron 30.0%
! CTTTTTACCTTGATTCAAAG+TGG - chr1_1:73671957-73671976 None:intergenic 30.0%
CACTTTGAATCAAGGTAAAA+AGG + chr1_1:73671955-73671974 Msa0042770:intron 30.0%
GGTAAAAAGGTAGATAGTTA+TGG + chr1_1:73671968-73671987 Msa0042770:intron 30.0%
!! GCTTGTTGTGCAAATATAAA+TGG - chr1_1:73672037-73672056 None:intergenic 30.0%
ATCCTGAAGGAAAAAAAGAT+TGG + chr1_1:73672080-73672099 Msa0042770:intron 30.0%
!!! AACCAATCTTTTTTTCCTTC+AGG - chr1_1:73672085-73672104 None:intergenic 30.0%
TGAAGGAAAAAAAGATTGGT+TGG + chr1_1:73672084-73672103 Msa0042770:intron 30.0%
! AACTTTAGAAGATAGTAAGC+CGG + chr1_1:73672137-73672156 Msa0042770:CDS 30.0%
! ATTGTGTGATTGATTCATCT+TGG - chr1_1:73672179-73672198 None:intergenic 30.0%
ATTGCAAATGACAATCTTGA+AGG + chr1_1:73672210-73672229 Msa0042770:CDS 30.0%
ATCATCTTCTTCTTCTTCTT+CGG - chr1_1:73672343-73672362 None:intergenic 30.0%
CCTTTAACATACGAAGATAA+TGG - chr1_1:73671122-73671141 None:intergenic 30.0%
CCATTATCTTCGTATGTTAA+AGG + chr1_1:73671119-73671138 Msa0042770:intron 30.0%
TACCGAGCTATTAGAAAAAA+GGG + chr1_1:73671389-73671408 Msa0042770:intron 30.0%
!!! TTCCCTTTTTTCTAATAGCT+CGG - chr1_1:73671394-73671413 None:intergenic 30.0%
AAGAAAAGAATTATGTGGTG+TGG - chr1_1:73671504-73671523 None:intergenic 30.0%
TAGGGAAGAAAAGAATTATG+TGG - chr1_1:73671509-73671528 None:intergenic 30.0%
! TTGAAGTTTACTATAGTGGA+TGG + chr1_1:73671911-73671930 Msa0042770:intron 30.0%
! CTTTTTACCTTGATTCAAAG+TGG - chr1_1:73671957-73671976 None:intergenic 30.0%
CACTTTGAATCAAGGTAAAA+AGG + chr1_1:73671955-73671974 Msa0042770:intron 30.0%
GGTAAAAAGGTAGATAGTTA+TGG + chr1_1:73671968-73671987 Msa0042770:intron 30.0%
!! GCTTGTTGTGCAAATATAAA+TGG - chr1_1:73672037-73672056 None:intergenic 30.0%
ATCCTGAAGGAAAAAAAGAT+TGG + chr1_1:73672080-73672099 Msa0042770:intron 30.0%
!!! AACCAATCTTTTTTTCCTTC+AGG - chr1_1:73672085-73672104 None:intergenic 30.0%
TGAAGGAAAAAAAGATTGGT+TGG + chr1_1:73672084-73672103 Msa0042770:intron 30.0%
! AACTTTAGAAGATAGTAAGC+CGG + chr1_1:73672137-73672156 Msa0042770:CDS 30.0%
! ATTGTGTGATTGATTCATCT+TGG - chr1_1:73672179-73672198 None:intergenic 30.0%
ATTGCAAATGACAATCTTGA+AGG + chr1_1:73672210-73672229 Msa0042770:CDS 30.0%
ATCATCTTCTTCTTCTTCTT+CGG - chr1_1:73672343-73672362 None:intergenic 30.0%
TCGTATGTTAAAGGATGAAC+AGG + chr1_1:73671128-73671147 Msa0042770:intron 35.0%
GTAGTTTATAAGACTCAGGA+AGG - chr1_1:73671187-73671206 None:intergenic 35.0%
CAATTCAGACTTAATGACTC+TGG - chr1_1:73671259-73671278 None:intergenic 35.0%
!!! TATTTGCATTTTGGTTGGAG+AGG + chr1_1:73671281-73671300 Msa0042770:intron 35.0%
ACTTAGGGGGAATTAAAAGA+CGG - chr1_1:73671352-73671371 None:intergenic 35.0%
!! TCTTTTAATTCCCCCTAAGT+TGG + chr1_1:73671352-73671371 Msa0042770:intron 35.0%
ATACAAGAAACCAACTTAGG+GGG - chr1_1:73671365-73671384 None:intergenic 35.0%
GATACAAGAAACCAACTTAG+GGG - chr1_1:73671366-73671385 None:intergenic 35.0%
GGATACAAGAAACCAACTTA+GGG - chr1_1:73671367-73671386 None:intergenic 35.0%
TGGATACAAGAAACCAACTT+AGG - chr1_1:73671368-73671387 None:intergenic 35.0%
! TTTTTCTAATAGCTCGGTAG+TGG - chr1_1:73671388-73671407 None:intergenic 35.0%
CTACCGAGCTATTAGAAAAA+AGG + chr1_1:73671388-73671407 Msa0042770:intron 35.0%
GCAATTGACAGTGATTACTA+TGG + chr1_1:73671871-73671890 Msa0042770:intron 35.0%
TTTGATCCCACTTTGAATCA+AGG + chr1_1:73671947-73671966 Msa0042770:intron 35.0%
! ATGTTGAAGTTAGAGCATCT+AGG + chr1_1:73671993-73672012 Msa0042770:intron 35.0%
TACTTCACATAGTATCCTGA+AGG + chr1_1:73672067-73672086 Msa0042770:intron 35.0%
! AAGTTTGAATTTTCTCGCCT+TGG - chr1_1:73672122-73672141 None:intergenic 35.0%
! GTGATTGATTCATCTTGGTA+TGG - chr1_1:73672174-73672193 None:intergenic 35.0%
!! GGAAGTCTTGTTGATTTAGT+TGG + chr1_1:73672231-73672250 Msa0042770:CDS 35.0%
TGATTTAGTTGGAGAAGTTG+AGG + chr1_1:73672242-73672261 Msa0042770:CDS 35.0%
TCGTATGTTAAAGGATGAAC+AGG + chr1_1:73671128-73671147 Msa0042770:intron 35.0%
GTAGTTTATAAGACTCAGGA+AGG - chr1_1:73671187-73671206 None:intergenic 35.0%
CAATTCAGACTTAATGACTC+TGG - chr1_1:73671259-73671278 None:intergenic 35.0%
!!! TATTTGCATTTTGGTTGGAG+AGG + chr1_1:73671281-73671300 Msa0042770:intron 35.0%
ACTTAGGGGGAATTAAAAGA+CGG - chr1_1:73671352-73671371 None:intergenic 35.0%
!! TCTTTTAATTCCCCCTAAGT+TGG + chr1_1:73671352-73671371 Msa0042770:intron 35.0%
ATACAAGAAACCAACTTAGG+GGG - chr1_1:73671365-73671384 None:intergenic 35.0%
GATACAAGAAACCAACTTAG+GGG - chr1_1:73671366-73671385 None:intergenic 35.0%
GGATACAAGAAACCAACTTA+GGG - chr1_1:73671367-73671386 None:intergenic 35.0%
TGGATACAAGAAACCAACTT+AGG - chr1_1:73671368-73671387 None:intergenic 35.0%
! TTTTTCTAATAGCTCGGTAG+TGG - chr1_1:73671388-73671407 None:intergenic 35.0%
CTACCGAGCTATTAGAAAAA+AGG + chr1_1:73671388-73671407 Msa0042770:intron 35.0%
GCAATTGACAGTGATTACTA+TGG + chr1_1:73671871-73671890 Msa0042770:intron 35.0%
TTTGATCCCACTTTGAATCA+AGG + chr1_1:73671947-73671966 Msa0042770:intron 35.0%
! ATGTTGAAGTTAGAGCATCT+AGG + chr1_1:73671993-73672012 Msa0042770:intron 35.0%
TACTTCACATAGTATCCTGA+AGG + chr1_1:73672067-73672086 Msa0042770:intron 35.0%
! AAGTTTGAATTTTCTCGCCT+TGG - chr1_1:73672122-73672141 None:intergenic 35.0%
! GTGATTGATTCATCTTGGTA+TGG - chr1_1:73672174-73672193 None:intergenic 35.0%
!! GGAAGTCTTGTTGATTTAGT+TGG + chr1_1:73672231-73672250 Msa0042770:CDS 35.0%
TGATTTAGTTGGAGAAGTTG+AGG + chr1_1:73672242-73672261 Msa0042770:CDS 35.0%
CTTAGGGGGAATTAAAAGAC+GGG - chr1_1:73671351-73671370 None:intergenic 40.0%
ACAAAAGATGCAGAGTTCAC+TGG + chr1_1:73671465-73671484 Msa0042770:intron 40.0%
TATTGTGGCGTATCTGTTCA+AGG + chr1_1:73671835-73671854 Msa0042770:intron 40.0%
! TAGTGGATGGCTAATGTGAT+TGG + chr1_1:73671924-73671943 Msa0042770:intron 40.0%
TGGTATGGAACTTCTTTCTC+CGG - chr1_1:73672159-73672178 None:intergenic 40.0%
AAGGTAACCTCATCCAAGAA+AGG - chr1_1:73672279-73672298 None:intergenic 40.0%
TGGATGAGGTTACCTTTGAA+TGG + chr1_1:73672283-73672302 Msa0042770:CDS 40.0%
GGATGAGGTTACCTTTGAAT+GGG + chr1_1:73672284-73672303 Msa0042770:CDS 40.0%
CTTAGGGGGAATTAAAAGAC+GGG - chr1_1:73671351-73671370 None:intergenic 40.0%
ACAAAAGATGCAGAGTTCAC+TGG + chr1_1:73671465-73671484 Msa0042770:intron 40.0%
TATTGTGGCGTATCTGTTCA+AGG + chr1_1:73671835-73671854 Msa0042770:intron 40.0%
! TAGTGGATGGCTAATGTGAT+TGG + chr1_1:73671924-73671943 Msa0042770:intron 40.0%
TGGTATGGAACTTCTTTCTC+CGG - chr1_1:73672159-73672178 None:intergenic 40.0%
AAGGTAACCTCATCCAAGAA+AGG - chr1_1:73672279-73672298 None:intergenic 40.0%
TGGATGAGGTTACCTTTGAA+TGG + chr1_1:73672283-73672302 Msa0042770:CDS 40.0%
GGATGAGGTTACCTTTGAAT+GGG + chr1_1:73672284-73672303 Msa0042770:CDS 40.0%
GTTAAAGGATGAACAGGTGC+AGG + chr1_1:73671134-73671153 Msa0042770:intron 45.0%
GTTGGCTGTGATTAAGACCA+AGG + chr1_1:73672102-73672121 Msa0042770:intron 45.0%
! GGAGAAGTTGAGGAAGCATA+CGG + chr1_1:73672252-73672271 Msa0042770:CDS 45.0%
ATACGGTCCTTTCTTGGATG+AGG + chr1_1:73672269-73672288 Msa0042770:CDS 45.0%
GTTAAAGGATGAACAGGTGC+AGG + chr1_1:73671134-73671153 Msa0042770:intron 45.0%
GTTGGCTGTGATTAAGACCA+AGG + chr1_1:73672102-73672121 Msa0042770:intron 45.0%
! GGAGAAGTTGAGGAAGCATA+CGG + chr1_1:73672252-73672271 Msa0042770:CDS 45.0%
ATACGGTCCTTTCTTGGATG+AGG + chr1_1:73672269-73672288 Msa0042770:CDS 45.0%
TGTGGCGTATCTGTTCAAGG+TGG + chr1_1:73671838-73671857 Msa0042770:intron 50.0%
CGTATCTGTTCAAGGTGGTG+AGG + chr1_1:73671843-73671862 Msa0042770:intron 50.0%
TCTGTTCAAGGTGGTGAGGA+TGG + chr1_1:73671847-73671866 Msa0042770:intron 50.0%
CTGTTCAAGGTGGTGAGGAT+GGG + chr1_1:73671848-73671867 Msa0042770:intron 50.0%
TGTTCAAGGTGGTGAGGATG+GGG + chr1_1:73671849-73671868 Msa0042770:intron 50.0%
! GGAAGCATACGGTCCTTTCT+TGG + chr1_1:73672263-73672282 Msa0042770:CDS 50.0%
TGTGGCGTATCTGTTCAAGG+TGG + chr1_1:73671838-73671857 Msa0042770:intron 50.0%
CGTATCTGTTCAAGGTGGTG+AGG + chr1_1:73671843-73671862 Msa0042770:intron 50.0%
TCTGTTCAAGGTGGTGAGGA+TGG + chr1_1:73671847-73671866 Msa0042770:intron 50.0%
CTGTTCAAGGTGGTGAGGAT+GGG + chr1_1:73671848-73671867 Msa0042770:intron 50.0%
TGTTCAAGGTGGTGAGGATG+GGG + chr1_1:73671849-73671868 Msa0042770:intron 50.0%
! GGAAGCATACGGTCCTTTCT+TGG + chr1_1:73672263-73672282 Msa0042770:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 73671102 73672398 73671102 ID=Msa0042770;Name=Msa0042770
chr1_1 mRNA 73671102 73672398 73671102 ID=Msa0042770-mRNA-1;Parent=Msa0042770;Name=Msa0042770-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|0.5|1|1|2|0|96
chr1_1 exon 73671102 73671111 73671102 ID=Msa0042770-mRNA-1:exon:24680;Parent=Msa0042770-mRNA-1
chr1_1 exon 73672118 73672398 73672118 ID=Msa0042770-mRNA-1:exon:24681;Parent=Msa0042770-mRNA-1
chr1_1 CDS 73671102 73671111 73671102 ID=Msa0042770-mRNA-1:cds;Parent=Msa0042770-mRNA-1
chr1_1 CDS 73672118 73672398 73672118 ID=Msa0042770-mRNA-1:cds;Parent=Msa0042770-mRNA-1
Gene Sequence

>Msa0042770

ATGTCTAAAGACCAAGGCGAGAAAATTCAAACTTTAGAAGATAGTAAGCCGGAGAAAGAAGTTCCATACCAAGATGAATCAATCACACAATCACACGTGACAATTGCAAATGACAATCTTGAAGGAAGTCTTGTTGATTTAGTTGGAGAAGTTGAGGAAGCATACGGTCCTTTCTTGGATGAGGTTACCTTTGAATGGGATAAAGATATTGAAGTAGAAGTAGAAGATAGTGCCGAAGAAGAAGAAGAAGATGATGATGATGATGATGATGATGAAACACATTCTGACTAG

Protein sequence

>Msa0042770

MSKDQGEKIQTLEDSKPEKEVPYQDESITQSHVTIANDNLEGSLVDLVGEVEEAYGPFLDEVTFEWDKDIEVEVEDSAEEEEEDDDDDDDDETHSD*