Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0805690 | XP_024639148.1 | 91.584 | 202 | 17 | 0 | 12 | 213 | 371 | 572 | 1.22e-128 | 384 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0805690 | G7K1E3 | 91.584 | 202 | 17 | 0 | 12 | 213 | 370 | 571 | 7.90e-129 | 384 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0795870 | Msa0805690 | 0.812743 | 3.635085e-51 | -8.615850e-47 |
| Msa0805690 | Msa0833650 | 0.811456 | 6.938566e-51 | -8.615850e-47 |
| Msa0805690 | Msa1132840 | 0.806532 | 7.860429e-50 | -8.615850e-47 |
| Msa0805690 | Msa1343170 | 0.800495 | 1.403449e-48 | -8.615850e-47 |
| Msa0805690 | Msa1343180 | 0.801157 | 1.027788e-48 | -8.615850e-47 |
| Msa0805690 | Msa1437930 | 0.810154 | 1.327174e-50 | -8.615850e-47 |
| Msa0190570 | Msa0805690 | 0.805431 | 1.339691e-49 | -8.615850e-47 |
| Msa0580370 | Msa0805690 | 0.806442 | 8.210833e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0805690 | MtrunA17_Chr5g0413621 | 91.584 | 202 | 17 | 0 | 12 | 213 | 370 | 571 | 1.52e-132 | 384 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0805690 | AT1G70340.1 | 44.651 | 215 | 81 | 6 | 11 | 213 | 322 | 510 | 3.17e-48 | 165 |
| Msa0805690 | AT1G23790.1 | 47.805 | 205 | 70 | 7 | 12 | 213 | 348 | 518 | 8.25e-46 | 159 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAAATCAAAAGGAGATTT+TGG | 0.171404 | 5_3:+22389829 | Msa0805690:CDS |
| AGAAGCTGGGAATTGGTTTA+TGG | 0.224742 | 5_3:+22389958 | Msa0805690:CDS |
| CGAGCAATCTGCACTGCTTT+AGG | 0.240173 | 5_3:-22390130 | None:intergenic |
| GGAGTTTCCTGATTGGTCTA+TGG | 0.360089 | 5_3:-22389735 | None:intergenic |
| TTGAAACAGAAGCTGGGAAT+TGG | 0.368398 | 5_3:+22389951 | Msa0805690:CDS |
| TTATACTTAAAGTTATGAAC+TGG | 0.369520 | 5_3:+22390065 | Msa0805690:CDS |
| GGCTAGGCCTCTTATTACTA+TGG | 0.370646 | 5_3:-22390105 | None:intergenic |
| ATTCGGTGGAGTTTCCTGAT+TGG | 0.373974 | 5_3:-22389742 | None:intergenic |
| GGAATTCATCGAGAAGGCTT+TGG | 0.409546 | 5_3:+22389979 | Msa0805690:CDS |
| ATCGAGAAGGCTTTGGAAGC+TGG | 0.409777 | 5_3:+22389986 | Msa0805690:CDS |
| CTGCTTCTACAGTATCAGTA+TGG | 0.424249 | 5_3:-22389598 | None:intergenic |
| AAAACAAAGGAAGCGTCAAA+AGG | 0.430890 | 5_3:+22390016 | Msa0805690:CDS |
| AAACGAAGGTGTGTTTATAA+AGG | 0.439091 | 5_3:+22389776 | Msa0805690:CDS |
| CAAAATTCCATAGACCAATC+AGG | 0.466760 | 5_3:+22389728 | Msa0805690:CDS |
| AACAATACCATAGTAATAAG+AGG | 0.475846 | 5_3:+22390098 | Msa0805690:CDS |
| GGAGAAGAAAAGTTCTACCT+TGG | 0.478194 | 5_3:+22389850 | Msa0805690:CDS |
| AAACAAAGGAAGCGTCAAAA+GGG | 0.480804 | 5_3:+22390017 | Msa0805690:CDS |
| TAAAGTTATGAACTGGGTTG+AGG | 0.488202 | 5_3:+22390072 | Msa0805690:CDS |
| ACTTTAAGTATAAGTGATTG+AGG | 0.490591 | 5_3:-22390055 | None:intergenic |
| AGAGACTTCAGCTTCAGTAT+TGG | 0.490725 | 5_3:+22389646 | Msa0805690:CDS |
| GATACTGTAGAAGCAGTTAG+TGG | 0.497058 | 5_3:+22389605 | Msa0805690:CDS |
| AAACAGATTGAAACAGAAGC+TGG | 0.500125 | 5_3:+22389944 | Msa0805690:CDS |
| ATCATCGAGTACAAATCAAA+AGG | 0.504941 | 5_3:+22389820 | Msa0805690:CDS |
| CTTAAGCAACACAATCAAGC+TGG | 0.516269 | 5_3:+22389919 | Msa0805690:CDS |
| CTAAAGCAGTGCAGATTGCT+CGG | 0.536163 | 5_3:+22390131 | Msa0805690:CDS |
| CTTTAAGTATAAGTGATTGA+GGG | 0.548171 | 5_3:-22390054 | None:intergenic |
| AACAGATTGAAACAGAAGCT+GGG | 0.564973 | 5_3:+22389945 | Msa0805690:CDS |
| TGCACTGCTTTAGGATGGCT+AGG | 0.572732 | 5_3:-22390121 | None:intergenic |
| GTTTATGGAATTCATCGAGA+AGG | 0.578128 | 5_3:+22389973 | Msa0805690:CDS |
| CAATCTGCACTGCTTTAGGA+TGG | 0.593787 | 5_3:-22390126 | None:intergenic |
| TATACTTAAAGTTATGAACT+GGG | 0.600506 | 5_3:+22390066 | Msa0805690:CDS |
| TTAAGCAACACAATCAAGCT+GGG | 0.607478 | 5_3:+22389920 | Msa0805690:CDS |
| AACAAAGGAAGCGTCAAAAG+GGG | 0.651530 | 5_3:+22390018 | Msa0805690:CDS |
| AATCAAATGTGTCAAAACGA+AGG | 0.676051 | 5_3:+22389762 | Msa0805690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTGTTTATAAAGGAAAAAT+GGG | + | chr5_3:22389785-22389804 | Msa0805690:CDS | 20.0% |
| !! | TTATACTTAAAGTTATGAAC+TGG | + | chr5_3:22390065-22390084 | Msa0805690:CDS | 20.0% |
| !! | TATACTTAAAGTTATGAACT+GGG | + | chr5_3:22390066-22390085 | Msa0805690:CDS | 20.0% |
| ! | GTGTGTTTATAAAGGAAAAA+TGG | + | chr5_3:22389784-22389803 | Msa0805690:CDS | 25.0% |
| !! | TACAAATCAAAAGGAGATTT+TGG | + | chr5_3:22389829-22389848 | Msa0805690:CDS | 25.0% |
| !! | TTCTCATCATTTTCAACAAT+TGG | - | chr5_3:22389878-22389897 | None:intergenic | 25.0% |
| ! | CTTTAAGTATAAGTGATTGA+GGG | - | chr5_3:22390057-22390076 | None:intergenic | 25.0% |
| ! | ACTTTAAGTATAAGTGATTG+AGG | - | chr5_3:22390058-22390077 | None:intergenic | 25.0% |
| ! | AACAATACCATAGTAATAAG+AGG | + | chr5_3:22390098-22390117 | Msa0805690:CDS | 25.0% |
| !! | TCGTTTTGACACATTTGATT+CGG | - | chr5_3:22389762-22389781 | None:intergenic | 30.0% |
| AATCAAATGTGTCAAAACGA+AGG | + | chr5_3:22389762-22389781 | Msa0805690:CDS | 30.0% | |
| ! | AAACGAAGGTGTGTTTATAA+AGG | + | chr5_3:22389776-22389795 | Msa0805690:CDS | 30.0% |
| ATAAAGGAAAAATGGGAAAG+CGG | + | chr5_3:22389792-22389811 | Msa0805690:CDS | 30.0% | |
| ATCATCGAGTACAAATCAAA+AGG | + | chr5_3:22389820-22389839 | Msa0805690:CDS | 30.0% | |
| !! | ATTTTCAACAATTGGTTCCA+AGG | - | chr5_3:22389870-22389889 | None:intergenic | 30.0% |
| !! | AGCTGGTTTGAAAAAAACAA+AGG | + | chr5_3:22390003-22390022 | Msa0805690:CDS | 30.0% |
| CAAAATTCCATAGACCAATC+AGG | + | chr5_3:22389728-22389747 | Msa0805690:CDS | 35.0% | |
| !!! | TTTTGACACATTTGATTCGG+TGG | - | chr5_3:22389759-22389778 | None:intergenic | 35.0% |
| TTAAGCAACACAATCAAGCT+GGG | + | chr5_3:22389920-22389939 | Msa0805690:CDS | 35.0% | |
| AAACAGATTGAAACAGAAGC+TGG | + | chr5_3:22389944-22389963 | Msa0805690:CDS | 35.0% | |
| AACAGATTGAAACAGAAGCT+GGG | + | chr5_3:22389945-22389964 | Msa0805690:CDS | 35.0% | |
| GTTTATGGAATTCATCGAGA+AGG | + | chr5_3:22389973-22389992 | Msa0805690:CDS | 35.0% | |
| !! | AAAACAAAGGAAGCGTCAAA+AGG | + | chr5_3:22390016-22390035 | Msa0805690:CDS | 35.0% |
| !! | AAACAAAGGAAGCGTCAAAA+GGG | + | chr5_3:22390017-22390036 | Msa0805690:CDS | 35.0% |
| TAAAGTTATGAACTGGGTTG+AGG | + | chr5_3:22390072-22390091 | Msa0805690:CDS | 35.0% | |
| !! | AGCTCGAGATCTGTTTTGAA+CGG | + | chr5_3:22389564-22389583 | Msa0805690:CDS | 40.0% |
| CTGCTTCTACAGTATCAGTA+TGG | - | chr5_3:22389601-22389620 | None:intergenic | 40.0% | |
| GATACTGTAGAAGCAGTTAG+TGG | + | chr5_3:22389605-22389624 | Msa0805690:CDS | 40.0% | |
| AGAGACTTCAGCTTCAGTAT+TGG | + | chr5_3:22389646-22389665 | Msa0805690:CDS | 40.0% | |
| ! | ACAATCGCATGTTTTACACG+AGG | + | chr5_3:22389700-22389719 | Msa0805690:CDS | 40.0% |
| GGAGAAGAAAAGTTCTACCT+TGG | + | chr5_3:22389850-22389869 | Msa0805690:CDS | 40.0% | |
| CTTAAGCAACACAATCAAGC+TGG | + | chr5_3:22389919-22389938 | Msa0805690:CDS | 40.0% | |
| TTGAAACAGAAGCTGGGAAT+TGG | + | chr5_3:22389951-22389970 | Msa0805690:CDS | 40.0% | |
| ! | AGAAGCTGGGAATTGGTTTA+TGG | + | chr5_3:22389958-22389977 | Msa0805690:CDS | 40.0% |
| !! | AACAAAGGAAGCGTCAAAAG+GGG | + | chr5_3:22390018-22390037 | Msa0805690:CDS | 40.0% |
| !! | GGAGTTTCCTGATTGGTCTA+TGG | - | chr5_3:22389738-22389757 | None:intergenic | 45.0% |
| ! | ATTCGGTGGAGTTTCCTGAT+TGG | - | chr5_3:22389745-22389764 | None:intergenic | 45.0% |
| GGAATTCATCGAGAAGGCTT+TGG | + | chr5_3:22389979-22389998 | Msa0805690:CDS | 45.0% | |
| GGCTAGGCCTCTTATTACTA+TGG | - | chr5_3:22390108-22390127 | None:intergenic | 45.0% | |
| !! | CAATCTGCACTGCTTTAGGA+TGG | - | chr5_3:22390129-22390148 | None:intergenic | 45.0% |
| CTAAAGCAGTGCAGATTGCT+CGG | + | chr5_3:22390131-22390150 | Msa0805690:CDS | 45.0% | |
| !! | ATCGAGAAGGCTTTGGAAGC+TGG | + | chr5_3:22389986-22390005 | Msa0805690:CDS | 50.0% |
| !! | TGCACTGCTTTAGGATGGCT+AGG | - | chr5_3:22390124-22390143 | None:intergenic | 50.0% |
| CGAGCAATCTGCACTGCTTT+AGG | - | chr5_3:22390133-22390152 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 22389542 | 22390183 | 22389542 | ID=Msa0805690;Name=Msa0805690 |
| chr5_3 | mRNA | 22389542 | 22390183 | 22389542 | ID=Msa0805690-mRNA-1;Parent=Msa0805690;Name=Msa0805690-mRNA-1;_AED=0.39;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|213 |
| chr5_3 | exon | 22389542 | 22390183 | 22389542 | ID=Msa0805690-mRNA-1:exon:8986;Parent=Msa0805690-mRNA-1 |
| chr5_3 | CDS | 22389542 | 22390183 | 22389542 | ID=Msa0805690-mRNA-1:cds;Parent=Msa0805690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
