AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0806160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806160 MtrunA17_Chr5g0414091 95.632 435 19 0 1 435 1 435 0.0 847
Msa0806160 MtrunA17_Chr5g0414041 64.828 435 151 2 1 435 5 437 0.0 560
Msa0806160 MtrunA17_Chr5g0414061 62.238 429 156 3 7 435 20 442 0.0 541
Msa0806160 MtrunA17_Chr5g0414051 66.914 405 114 6 34 435 37 424 0.0 540
Msa0806160 MtrunA17_Chr3g0140371 66.759 361 110 5 75 433 10 362 8.10e-178 501
Msa0806160 MtrunA17_Chr8g0391481 63.881 371 126 5 66 432 7 373 8.78e-176 496
Msa0806160 MtrunA17_Chr7g0226991 61.972 355 128 4 82 433 54 404 1.43e-162 464
Msa0806160 MtrunA17_Chr5g0414081 71.642 201 53 3 236 433 2 201 2.34e-104 308
Msa0806160 MtrunA17_Chr8g0347321 40.227 353 201 5 83 431 93 439 9.27e-97 297
Msa0806160 MtrunA17_Chr8g0387921 41.477 352 195 7 85 431 74 419 5.38e-95 292
Msa0806160 MtrunA17_Chr6g0456691 37.681 414 242 9 25 432 17 420 1.84e-94 291
Msa0806160 MtrunA17_Chr6g0456681 38.544 371 214 7 68 432 47 409 1.52e-84 265
Msa0806160 MtrunA17_Chr6g0451321 38.172 372 212 8 66 433 102 459 1.37e-83 264
Msa0806160 MtrunA17_Chr2g0283851 37.360 356 198 12 90 433 60 402 4.34e-76 243
Msa0806160 MtrunA17_Chr1g0188191 33.884 363 213 11 85 433 234 583 6.62e-65 218
Msa0806160 MtrunA17_Chr6g0476041 33.069 378 200 13 83 433 165 516 1.03e-63 214
Msa0806160 MtrunA17_Chr7g0259711 33.516 364 209 13 84 433 90 434 3.38e-53 184
Msa0806160 MtrunA17_Chr7g0221281 37.295 244 141 6 193 432 2 237 1.53e-51 174
Msa0806160 MtrunA17_Chr2g0287901 33.693 371 202 14 84 435 96 441 2.33e-50 176
Msa0806160 MtrunA17_Chr1g0175671 33.062 369 201 15 85 433 170 512 1.19e-46 168
Msa0806160 MtrunA17_Chr4g0071391 32.880 368 192 16 83 427 107 442 4.81e-46 165
Msa0806160 MtrunA17_Chr7g0258851 32.440 373 210 15 74 427 234 583 1.13e-44 164
Msa0806160 MtrunA17_Chr7g0269991 31.549 355 203 13 85 427 137 463 2.35e-43 158
Msa0806160 MtrunA17_Chr1g0213281 30.854 363 207 15 84 427 174 511 4.04e-43 158
Msa0806160 MtrunA17_Chr4g0068111 28.706 425 248 18 27 431 64 453 9.50e-43 156
Msa0806160 MtrunA17_Chr8g0384151 30.663 362 205 12 83 431 88 416 1.49e-42 155
Msa0806160 MtrunA17_Chr4g0070341 30.518 367 217 12 76 427 124 467 2.59e-42 155
Msa0806160 MtrunA17_Chr4g0005491 31.579 361 210 17 85 427 62 403 1.26e-41 152
Msa0806160 MtrunA17_Chr4g0006991 29.032 372 222 15 79 431 79 427 5.03e-41 151
Msa0806160 MtrunA17_Chr3g0091031 30.084 359 194 11 83 427 29 344 6.87e-41 149
Msa0806160 MtrunA17_Chr5g0430371 29.570 372 215 10 77 427 50 395 2.15e-40 149
Msa0806160 MtrunA17_Chr8g0360521 27.059 425 259 15 23 427 12 405 2.50e-40 149
Msa0806160 MtrunA17_Chr8g0368461 29.231 390 221 15 73 432 166 530 3.59e-40 150
Msa0806160 MtrunA17_Chr5g0413271 28.650 363 224 12 81 433 141 478 3.97e-40 149
Msa0806160 MtrunA17_Chr7g0243301 31.233 365 206 17 85 427 64 405 4.47e-40 148
Msa0806160 MtrunA17_Chr2g0314801 31.373 357 205 12 82 431 30 353 1.22e-38 142
Msa0806160 MtrunA17_Chr3g0085081 30.769 351 202 14 83 427 29 344 1.66e-38 142
Msa0806160 MtrunA17_Chr3g0126021 29.091 385 213 13 69 431 113 459 1.90e-38 144
Msa0806160 MtrunA17_Chr7g0242701 30.570 386 216 15 69 431 71 427 2.28e-38 144
Msa0806160 MtrunA17_Chr3g0113671 28.333 360 227 12 82 427 103 445 1.28e-37 142
Msa0806160 MtrunA17_Chr3g0105301 29.513 349 227 11 82 427 40 372 1.40e-37 140
Msa0806160 MtrunA17_Chr3g0085071 29.683 347 211 10 83 427 44 359 8.20e-37 138
Msa0806160 MtrunA17_Chr4g0049421 29.024 410 225 16 54 431 17 392 1.40e-36 138
Msa0806160 MtrunA17_Chr2g0314791 31.006 358 201 14 82 431 26 345 1.58e-36 137
Msa0806160 MtrunA17_Chr3g0097891 31.215 362 203 14 76 427 21 346 2.43e-36 136
Msa0806160 MtrunA17_Chr7g0221271 34.921 189 113 4 29 212 22 205 6.20e-36 131
Msa0806160 MtrunA17_Chr8g0384421 27.273 363 229 9 83 431 131 472 6.74e-36 137
Msa0806160 MtrunA17_Chr6g0455901 27.938 451 260 18 8 431 4 416 7.56e-36 137
Msa0806160 MtrunA17_Chr4g0045941 30.058 346 209 10 84 427 32 346 1.08e-34 132
Msa0806160 MtrunA17_Chr1g0178081 28.333 360 176 11 83 431 53 341 1.56e-34 132
Msa0806160 MtrunA17_Chr5g0437601 29.223 373 205 14 82 427 80 420 1.74e-34 133
Msa0806160 MtrunA17_Chr1g0171171 28.065 367 234 11 67 431 37 375 1.85e-34 132
Msa0806160 MtrunA17_Chr4g0045951 29.769 346 210 9 84 427 44 358 5.08e-34 130
Msa0806160 MtrunA17_Chr2g0325481 30.925 346 203 11 85 427 50 362 1.03e-33 129
Msa0806160 MtrunA17_Chr3g0114101 29.609 358 217 13 85 427 89 426 2.13e-33 130
Msa0806160 MtrunA17_Chr5g0413851 30.769 351 202 13 83 427 40 355 1.08e-32 127
Msa0806160 MtrunA17_Chr4g0041011 28.682 387 240 13 49 431 9 363 1.25e-32 126
Msa0806160 MtrunA17_Chr1g0210771 26.027 365 213 12 80 427 54 378 6.25e-32 125
Msa0806160 MtrunA17_Chr5g0437291 27.901 362 227 13 83 427 163 507 8.97e-32 127
Msa0806160 MtrunA17_Chr2g0283581 29.315 365 220 14 70 431 29 358 1.96e-31 123
Msa0806160 MtrunA17_Chr4g0005481 28.533 368 217 15 83 431 106 446 2.30e-31 124
Msa0806160 MtrunA17_Chr7g0221291 37.333 150 85 3 29 173 22 167 1.40e-28 110
Msa0806160 MtrunA17_Chr1g0209921 32.778 180 111 6 79 255 38 210 1.21e-20 91.3
Msa0806160 MtrunA17_Chr4g0057171 44.211 95 47 2 227 321 1 89 5.17e-19 82.8
Msa0806160 MtrunA17_Chr7g0275501 57.377 61 23 1 370 430 11 68 1.93e-18 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806160 AT1G70230.1 53.933 356 146 6 82 435 76 415 1.85e-146 424
Msa0806160 AT5G15890.1 39.535 387 215 8 50 432 152 523 1.55e-98 305
Msa0806160 AT1G01430.1 39.018 387 221 8 52 431 69 447 1.32e-92 287
Msa0806160 AT4G01080.1 38.191 398 223 8 35 429 51 428 8.09e-91 282
Msa0806160 AT3G28150.1 37.284 405 236 10 36 433 19 412 2.06e-90 280
Msa0806160 AT4G23790.1 36.215 428 260 9 10 431 7 427 2.83e-90 280
Msa0806160 AT3G02440.2 38.527 353 202 6 85 432 135 477 8.10e-90 281
Msa0806160 AT3G02440.1 38.527 353 202 6 85 432 127 469 8.12e-90 281
Msa0806160 AT4G11090.1 36.343 432 254 12 9 431 6 425 2.00e-88 276
Msa0806160 AT5G15900.1 36.939 379 221 10 65 435 45 413 3.75e-82 259
Msa0806160 AT5G15900.2 36.939 379 221 10 65 435 64 432 5.63e-82 259
Msa0806160 AT4G25360.1 33.920 398 232 12 51 433 145 526 5.52e-70 231
Msa0806160 AT4G25360.2 33.920 398 232 12 51 433 145 526 5.52e-70 231
Msa0806160 AT5G51640.1 33.760 391 224 14 63 433 115 490 4.36e-66 219
Msa0806160 AT5G19160.1 34.426 366 209 11 85 435 99 448 1.32e-55 191
Msa0806160 AT3G06080.2 32.967 364 214 12 85 435 103 449 2.97e-51 179
Msa0806160 AT3G12060.1 31.915 376 212 16 77 433 188 538 4.31e-49 176
Msa0806160 AT1G48880.1 34.301 379 191 18 71 428 95 436 1.30e-47 169
Msa0806160 AT5G06700.1 32.213 357 211 14 83 427 253 590 2.60e-46 169
Msa0806160 AT5G64020.1 31.081 370 214 12 85 431 57 408 6.76e-46 164
Msa0806160 AT5G20590.1 31.892 370 210 14 75 427 131 475 1.96e-45 164
Msa0806160 AT3G11570.1 32.249 369 200 15 85 431 79 419 3.93e-44 159
Msa0806160 AT2G14530.1 29.524 420 235 19 36 431 24 406 6.41e-43 156
Msa0806160 AT5G49340.1 30.194 361 213 12 84 427 99 437 4.49e-42 155
Msa0806160 AT3G62390.1 29.661 354 218 12 83 427 136 467 1.11e-41 154
Msa0806160 AT5G58600.1 29.319 382 216 11 68 431 55 400 3.14e-41 151
Msa0806160 AT3G14850.2 32.591 359 204 11 79 431 28 354 5.65e-41 149
Msa0806160 AT5G06230.2 30.645 372 209 14 83 431 19 364 1.89e-40 148
Msa0806160 AT5G06230.1 30.645 372 209 14 83 431 60 405 2.89e-40 149
Msa0806160 AT2G31110.2 29.722 360 217 13 72 427 31 358 6.08e-39 144
Msa0806160 AT2G38320.1 28.729 362 229 12 80 427 54 400 4.08e-38 143
Msa0806160 AT5G20680.6 28.218 404 232 12 57 431 83 457 1.26e-37 142
Msa0806160 AT1G60790.1 27.989 368 226 13 83 432 188 534 2.23e-37 143
Msa0806160 AT2G37720.1 28.804 368 217 11 83 431 141 482 2.52e-37 142
Msa0806160 AT5G20680.4 28.218 404 232 12 57 431 177 551 2.98e-37 143
Msa0806160 AT5G20680.1 28.218 404 232 12 57 431 177 551 2.98e-37 143
Msa0806160 AT5G20680.3 28.218 404 232 12 57 431 177 551 2.98e-37 143
Msa0806160 AT5G20680.5 28.218 404 232 12 57 431 177 551 2.98e-37 143
Msa0806160 AT5G20680.2 28.218 404 232 12 57 431 159 533 3.06e-37 142
Msa0806160 AT1G73140.1 29.759 373 218 17 72 427 56 401 3.52e-37 140
Msa0806160 AT2G30010.1 28.947 380 204 10 82 431 53 396 5.58e-37 139
Msa0806160 AT5G01360.2 28.532 361 224 13 83 428 34 375 7.00e-37 139
Msa0806160 AT2G30010.2 29.551 379 199 12 84 431 98 439 7.08e-37 140
Msa0806160 AT2G30900.1 29.261 352 213 11 82 427 40 361 1.37e-36 138
Msa0806160 AT3G55990.1 29.363 361 220 13 83 427 138 479 1.71e-36 140
Msa0806160 AT5G01360.1 28.532 361 224 13 83 428 90 431 2.16e-36 139
Msa0806160 AT5G64470.3 28.969 359 224 13 85 431 55 394 2.56e-36 138
Msa0806160 AT5G19160.2 35.772 246 139 8 85 320 99 335 3.87e-36 136
Msa0806160 AT2G40320.1 28.846 364 218 13 82 427 78 418 3.60e-35 135
Msa0806160 AT2G40160.2 26.995 426 259 16 26 427 22 419 3.81e-35 135
Msa0806160 AT2G42570.1 29.915 351 209 12 85 431 47 364 3.93e-35 134
Msa0806160 AT3G11030.1 27.654 358 229 11 83 427 104 444 3.96e-35 135
Msa0806160 AT2G40160.1 26.995 426 259 16 26 427 22 419 4.11e-35 135
Msa0806160 AT5G64470.2 28.691 359 231 13 85 431 55 400 7.41e-35 134
Msa0806160 AT2G40150.1 29.199 387 228 16 63 427 54 416 2.14e-34 133
Msa0806160 AT2G38320.2 29.141 326 203 11 115 427 7 317 3.74e-34 130
Msa0806160 AT1G29050.1 28.611 360 222 12 75 431 51 378 1.18e-33 130
Msa0806160 AT2G34070.1 27.841 352 218 12 84 431 64 383 7.41e-33 128
Msa0806160 AT3G06080.1 33.065 248 145 7 85 321 103 340 3.88e-32 125
Msa0806160 AT3G54260.1 26.741 359 216 12 85 431 51 374 5.58e-31 122
Msa0806160 AT1G78710.1 30.471 361 213 15 76 431 29 356 1.34e-30 121
Msa0806160 AT3G62390.2 29.268 246 161 6 83 322 136 374 4.26e-29 118
Msa0806160 AT5G01620.2 26.933 375 227 14 77 431 102 449 4.32e-29 119
Msa0806160 AT5G01620.1 26.933 375 227 14 77 431 102 449 4.32e-29 119
Msa0806160 AT5G01620.3 26.933 375 227 14 77 431 110 457 4.81e-29 119
Msa0806160 AT5G20590.2 31.801 261 151 10 75 321 131 378 5.68e-29 117
Msa0806160 AT5G58600.2 30.418 263 144 8 68 319 55 289 4.93e-26 107
Msa0806160 AT5G06230.3 31.538 260 143 11 83 321 60 305 4.02e-25 105
Msa0806160 AT2G31110.3 33.152 184 115 4 72 254 31 207 1.21e-24 102
Msa0806160 AT2G40160.3 31.771 192 121 4 26 214 22 206 1.22e-23 101
Msa0806160 AT2G30900.2 33.714 175 109 3 82 255 40 208 6.27e-23 98.2
Msa0806160 AT5G64470.4 28.137 263 169 10 85 335 55 309 6.72e-20 90.5
Msa0806160 AT5G64470.1 28.137 263 169 10 85 335 55 309 8.18e-20 90.5
Msa0806160 AT3G14850.1 30.142 282 159 11 156 431 2 251 1.00e-19 88.6
Msa0806160 AT1G78710.2 31.667 180 114 4 76 254 22 193 1.65e-18 86.3
Msa0806160 AT2G31110.1 27.602 221 120 12 222 427 15 210 9.36e-11 62.0

Find 106 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTCAATTATACTATTA+AGG 0.098289 5_3:-23662686 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.120096 5_3:-23662370 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5_3:+23662169 Msa0806160:CDS
GTCCTCAAAGCCTTTCTTAA+AGG 0.191336 5_3:-23662660 None:intergenic
TATGAGAGTGATTTAGTTCT+TGG 0.213205 5_3:+23662588 Msa0806160:CDS
GCTTAGAAAGGGTTTGAAAA+TGG 0.214590 5_3:+23661566 Msa0806160:exon
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5_3:-23662299 None:intergenic
ACAAATGGGAGACCTGATTT+AGG 0.292037 5_3:+23662156 Msa0806160:CDS
CCTCTCAATTATACTATTAA+GGG 0.309841 5_3:-23662685 None:intergenic
CTTAGAAAGGGTTTGAAAAT+GGG 0.349836 5_3:+23661567 Msa0806160:exon
TCAACACCATGCACTAGAAA+TGG 0.356439 5_3:-23662432 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5_3:+23662841 Msa0806160:CDS
TTGTAGAGATGATGAAGAAA+TGG 0.363410 5_3:+23663087 Msa0806160:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5_3:-23661666 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5_3:+23662061 Msa0806160:CDS
TCACTCTCATAGTAAATTGA+TGG 0.370160 5_3:-23662576 None:intergenic
AGGAAAGTTGCTAAAGGAAA+TGG 0.381708 5_3:+23662705 Msa0806160:CDS
GGTGTTGGATGTAACTAAAT+TGG 0.388499 5_3:+23662929 Msa0806160:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5_3:-23662101 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5_3:+23662816 Msa0806160:CDS
ATTTATTGGACTTGCCATCT+TGG 0.403569 5_3:-23662356 None:intergenic
CAACACCATGCACTAGAAAT+GGG 0.415330 5_3:-23662431 None:intergenic
TTCCTTTAAGAAAGGCTTTG+AGG 0.416940 5_3:+23662658 Msa0806160:CDS
CAACATAAAACAGTGATTAG+AGG 0.421138 5_3:+23661532 Msa0806160:five_prime_UTR
GGCAAAGGATATTGATCAAA+TGG 0.427874 5_3:+23662515 Msa0806160:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5_3:-23662999 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5_3:+23662824 Msa0806160:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5_3:-23662993 None:intergenic
AGTGAGAGATGCATCACAAA+TGG 0.437580 5_3:+23662141 Msa0806160:CDS
CCATCTTGGTAAACACGGTT+AGG 0.438862 5_3:-23662342 None:intergenic
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5_3:-23661758 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5_3:-23662102 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5_3:-23662168 None:intergenic
ACAAAAGCTAATAAATTTGA+AGG 0.447815 5_3:+23662894 Msa0806160:CDS
GTTTGTTCTTAATGAGATTG+AGG 0.450023 5_3:-23662242 None:intergenic
TGTAGAGATGATGAAGAAAT+GGG 0.451545 5_3:+23663088 Msa0806160:CDS
CTTGGTAGATTACACTCACT+TGG 0.451639 5_3:-23662201 None:intergenic
TGACTATTCAAATGGAGATT+GGG 0.453167 5_3:+23662062 Msa0806160:CDS
TGTGTTCATTGGTGCTTGCC+AGG 0.456446 5_3:+23663038 Msa0806160:CDS
ATTTGAAGGTATTAGAATTG+AGG 0.457268 5_3:+23662908 Msa0806160:CDS
TACAAAACGATTGTGTTCAT+TGG 0.458956 5_3:+23663027 Msa0806160:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5_3:+23662175 Msa0806160:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5_3:-23662963 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5_3:+23662819 Msa0806160:CDS
AAATCATGTGACTATTCAAA+TGG 0.471379 5_3:+23662054 Msa0806160:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5_3:+23662957 Msa0806160:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5_3:+23662426 Msa0806160:CDS
TAGTTGGTCCAAAAGGAGTA+AGG 0.483038 5_3:-23661704 None:intergenic
AGAAGCTTAGTTGGTCCAAA+AGG 0.489769 5_3:-23661711 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5_3:+23662803 Msa0806160:CDS
AAGGATTGCTATAGAAGAAG+TGG 0.492816 5_3:+23662860 Msa0806160:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5_3:-23662981 None:intergenic
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5_3:+23662828 Msa0806160:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5_3:+23663054 Msa0806160:CDS
GGATGAAGTATAAACAAAGA+AGG 0.502496 5_3:-23661674 None:intergenic
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5_3:-23661601 None:intergenic
CATGAAAGAAAGCTTAGAAA+GGG 0.505195 5_3:+23661555 Msa0806160:five_prime_UTR
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5_3:-23661754 None:intergenic
GTAGAATGAAGAAGCTTAGT+TGG 0.508993 5_3:-23661720 None:intergenic
TTGTTGGGGATTCTTTGGCT+AGG 0.510878 5_3:+23662274 Msa0806160:CDS
CAAAAGGAGTAAGGGACAAA+AGG 0.511314 5_3:-23661695 None:intergenic
AAAGTGCTTGGTTCAAACCT+TGG 0.519498 5_3:-23662219 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5_3:+23662768 Msa0806160:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5_3:+23662447 Msa0806160:CDS
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5_3:-23661600 None:intergenic
TGGGGACCAATACAAAGAAA+AGG 0.526383 5_3:-23662412 None:intergenic
GTGAGAGATGCATCACAAAT+GGG 0.526674 5_3:+23662142 Msa0806160:CDS
GCATGAAAGAAAGCTTAGAA+AGG 0.526820 5_3:+23661554 Msa0806160:five_prime_UTR
GATGAAGTATAAACAAAGAA+GGG 0.527867 5_3:-23661673 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5_3:+23662367 Msa0806160:CDS
GAGTCATGAAAGGAAGAGAA+AGG 0.534479 5_3:-23661477 None:intergenic
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5_3:-23663056 None:intergenic
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5_3:-23661786 None:intergenic
ATGCATCACCCTTCTCTATG+TGG 0.539115 5_3:-23663119 None:intergenic
GGATCTTGTCAATGAGAAGT+GGG 0.539423 5_3:+23662494 Msa0806160:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.540214 5_3:-23663062 None:intergenic
CAATCACCTTCAAAATGAGC+AGG 0.550833 5_3:-23662756 None:intergenic
ATGAGATTGAGGAAAGTGCT+TGG 0.551052 5_3:-23662231 None:intergenic
CCTAACCGTGTTTACCAAGA+TGG 0.559444 5_3:+23662342 Msa0806160:CDS
TTCTTTGATTGTGCCACATG+TGG 0.561553 5_3:-23662118 None:intergenic
AGGTATTAGAATTGAGGTGT+TGG 0.565513 5_3:+23662914 Msa0806160:CDS
TGGATCTTGTCAATGAGAAG+TGG 0.565608 5_3:+23662493 Msa0806160:CDS
ATTGAGAGGAAAGTTGCTAA+AGG 0.566358 5_3:+23662699 Msa0806160:CDS
GAAAAGGTAGCATTATGAGA+AGG 0.570303 5_3:-23662396 None:intergenic
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5_3:-23661599 None:intergenic
TGAATAGTGGTAGGTGGCAA+TGG 0.576540 5_3:-23661745 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5_3:+23662948 Msa0806160:CDS
GAAAGGGTTTGAAAATGGGA+AGG 0.593746 5_3:+23661571 Msa0806160:exon
AAAGGATTCATGTAAGGACC+TGG 0.594011 5_3:-23662975 None:intergenic
CTTTATGAGAGAGTCATGAA+AGG 0.600156 5_3:-23661487 None:intergenic
GAGTAACATCCACATAGAGA+AGG 0.608356 5_3:+23663110 Msa0806160:exon
AAAGGGTTTGAAAATGGGAA+GGG 0.612498 5_3:+23661572 Msa0806160:exon
AGTAGATGAATAGTGGTAGG+TGG 0.614813 5_3:-23661751 None:intergenic
TGTCAATGAGAAGTGGGCAA+AGG 0.618169 5_3:+23662500 Msa0806160:CDS
AGTTGGTCCAAAAGGAGTAA+GGG 0.618383 5_3:-23661703 None:intergenic
ACTTGCCATCTTGGTAAACA+CGG 0.618682 5_3:-23662347 None:intergenic
AGTAACATCCACATAGAGAA+GGG 0.625804 5_3:+23663111 Msa0806160:exon
ATATACAATGTCACCACATG+TGG 0.627135 5_3:+23662105 Msa0806160:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5_3:+23662448 Msa0806160:CDS
ATTTGAGTATGATTAAACTC+AGG 0.636695 5_3:-23662624 None:intergenic
CAAGTGAGTGTAATCTACCA+AGG 0.650297 5_3:+23662202 Msa0806160:CDS
CCCTTAATAGTATAATTGAG+AGG 0.654229 5_3:+23662685 Msa0806160:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5_3:-23661783 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5_3:-23662430 None:intergenic
GATTGGGTTAGTGACATGAG+AGG 0.690038 5_3:+23662078 Msa0806160:CDS
ATAGTGGTAGGTGGCAATGG+TGG 0.693322 5_3:-23661742 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG + chr5_3:23662473-23662492 Msa0806160:CDS 10.0%
!!! TTATTATAATACAATGTTTT+TGG + chr5_3:23662473-23662492 Msa0806160:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG - chr5_3:23661625-23661644 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG + chr5_3:23662006-23662025 Msa0806160:intron 15.0%
!!! TATGTAATATATTTTTTGCA+GGG + chr5_3:23662007-23662026 Msa0806160:intron 15.0%
!! ATGAAAAAATTTCTTATTGA+AGG - chr5_3:23661625-23661644 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG + chr5_3:23662006-23662025 Msa0806160:intron 15.0%
!!! TATGTAATATATTTTTTGCA+GGG + chr5_3:23662007-23662026 Msa0806160:intron 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG - chr5_3:23661622-23661641 None:intergenic 20.0%
!!! TTTTTATGTTCCTTTAAGAA+AGG + chr5_3:23662650-23662669 Msa0806160:CDS 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG - chr5_3:23662892-23662911 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG + chr5_3:23662894-23662913 Msa0806160:CDS 20.0%
!! AAAAAATTTCTTATTGAAGG+AGG - chr5_3:23661622-23661641 None:intergenic 20.0%
!!! TTTTTATGTTCCTTTAAGAA+AGG + chr5_3:23662650-23662669 Msa0806160:CDS 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG - chr5_3:23662892-23662911 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG + chr5_3:23662894-23662913 Msa0806160:CDS 20.0%
! TGTTGAGACAAAAAATCAAT+GGG + chr5_3:23661451-23661470 Msa0806160:five_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG - chr5_3:23661669-23661688 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG - chr5_3:23661676-23661695 None:intergenic 25.0%
! AAATCATGTGACTATTCAAA+TGG + chr5_3:23662054-23662073 Msa0806160:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG - chr5_3:23662171-23662190 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG + chr5_3:23662169-23662188 Msa0806160:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG + chr5_3:23662258-23662277 Msa0806160:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG + chr5_3:23662259-23662278 Msa0806160:CDS 25.0%
!!! ACATGATTGTTTTATCAACT+GGG + chr5_3:23662538-23662557 Msa0806160:CDS 25.0%
! ATTTGAGTATGATTAAACTC+AGG - chr5_3:23662627-23662646 None:intergenic 25.0%
! CCTCTCAATTATACTATTAA+GGG - chr5_3:23662688-23662707 None:intergenic 25.0%
! TCCTCTCAATTATACTATTA+AGG - chr5_3:23662689-23662708 None:intergenic 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG - chr5_3:23662813-23662832 None:intergenic 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG + chr5_3:23662908-23662927 Msa0806160:CDS 25.0%
!! CAATCGTTTTGTACATATTT+TGG - chr5_3:23663020-23663039 None:intergenic 25.0%
! TGTTGAGACAAAAAATCAAT+GGG + chr5_3:23661451-23661470 Msa0806160:five_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG - chr5_3:23661669-23661688 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG - chr5_3:23661676-23661695 None:intergenic 25.0%
! AAATCATGTGACTATTCAAA+TGG + chr5_3:23662054-23662073 Msa0806160:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG - chr5_3:23662171-23662190 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG + chr5_3:23662169-23662188 Msa0806160:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG + chr5_3:23662258-23662277 Msa0806160:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG + chr5_3:23662259-23662278 Msa0806160:CDS 25.0%
!!! ACATGATTGTTTTATCAACT+GGG + chr5_3:23662538-23662557 Msa0806160:CDS 25.0%
! ATTTGAGTATGATTAAACTC+AGG - chr5_3:23662627-23662646 None:intergenic 25.0%
! CCTCTCAATTATACTATTAA+GGG - chr5_3:23662688-23662707 None:intergenic 25.0%
! TCCTCTCAATTATACTATTA+AGG - chr5_3:23662689-23662708 None:intergenic 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG - chr5_3:23662813-23662832 None:intergenic 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG + chr5_3:23662908-23662927 Msa0806160:CDS 25.0%
!! CAATCGTTTTGTACATATTT+TGG - chr5_3:23663020-23663039 None:intergenic 25.0%
GTGTTGAGACAAAAAATCAA+TGG + chr5_3:23661450-23661469 Msa0806160:five_prime_UTR 30.0%
! TCACTGTTTTATGTTGTTGT+CGG - chr5_3:23661528-23661547 None:intergenic 30.0%
CAACATAAAACAGTGATTAG+AGG + chr5_3:23661532-23661551 Msa0806160:five_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG + chr5_3:23661555-23661574 Msa0806160:five_prime_UTR 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG + chr5_3:23661567-23661586 Msa0806160:exon 30.0%
GGATGAAGTATAAACAAAGA+AGG - chr5_3:23661677-23661696 None:intergenic 30.0%
AAGAGTAAGTAGATGAATAG+TGG - chr5_3:23661761-23661780 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG - chr5_3:23661789-23661808 None:intergenic 30.0%
CCTTAATAGTAACAAATTGC+TGG - chr5_3:23661966-23661985 None:intergenic 30.0%
!! CCAGCAATTTGTTACTATTA+AGG + chr5_3:23661963-23661982 Msa0806160:intron 30.0%
TGTTACTATTAAGGACTTCT+TGG + chr5_3:23661972-23661991 Msa0806160:intron 30.0%
TGACTATTCAAATGGAGATT+GGG + chr5_3:23662062-23662081 Msa0806160:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG - chr5_3:23662104-23662123 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG + chr5_3:23662175-23662194 Msa0806160:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG - chr5_3:23662245-23662264 None:intergenic 30.0%
! CAAACATATAGCTTTTGTTG+GGG + chr5_3:23662260-23662279 Msa0806160:CDS 30.0%
AGTCCAATAAATTTCGTAAG+TGG + chr5_3:23662367-23662386 Msa0806160:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG + chr5_3:23662406-23662425 Msa0806160:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG + chr5_3:23662448-23662467 Msa0806160:CDS 30.0%
!! GACATGATTGTTTTATCAAC+TGG + chr5_3:23662537-23662556 Msa0806160:CDS 30.0%
TCACTCTCATAGTAAATTGA+TGG - chr5_3:23662579-23662598 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG + chr5_3:23662588-23662607 Msa0806160:CDS 30.0%
CCCTTAATAGTATAATTGAG+AGG + chr5_3:23662685-23662704 Msa0806160:CDS 30.0%
AGAAAAGAGGATAAGATAGT+TGG + chr5_3:23662816-23662835 Msa0806160:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG - chr5_3:23662984-23663003 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG + chr5_3:23662990-23663009 Msa0806160:CDS 30.0%
TACAAAACGATTGTGTTCAT+TGG + chr5_3:23663027-23663046 Msa0806160:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG - chr5_3:23663065-23663084 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG + chr5_3:23663087-23663106 Msa0806160:CDS 30.0%
TGTAGAGATGATGAAGAAAT+GGG + chr5_3:23663088-23663107 Msa0806160:CDS 30.0%
GTGTTGAGACAAAAAATCAA+TGG + chr5_3:23661450-23661469 Msa0806160:five_prime_UTR 30.0%
! TCACTGTTTTATGTTGTTGT+CGG - chr5_3:23661528-23661547 None:intergenic 30.0%
CAACATAAAACAGTGATTAG+AGG + chr5_3:23661532-23661551 Msa0806160:five_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG + chr5_3:23661555-23661574 Msa0806160:five_prime_UTR 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG + chr5_3:23661567-23661586 Msa0806160:exon 30.0%
GGATGAAGTATAAACAAAGA+AGG - chr5_3:23661677-23661696 None:intergenic 30.0%
AAGAGTAAGTAGATGAATAG+TGG - chr5_3:23661761-23661780 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG - chr5_3:23661789-23661808 None:intergenic 30.0%
CCTTAATAGTAACAAATTGC+TGG - chr5_3:23661966-23661985 None:intergenic 30.0%
!! CCAGCAATTTGTTACTATTA+AGG + chr5_3:23661963-23661982 Msa0806160:intron 30.0%
TGTTACTATTAAGGACTTCT+TGG + chr5_3:23661972-23661991 Msa0806160:intron 30.0%
TGACTATTCAAATGGAGATT+GGG + chr5_3:23662062-23662081 Msa0806160:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG - chr5_3:23662104-23662123 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG + chr5_3:23662175-23662194 Msa0806160:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG - chr5_3:23662245-23662264 None:intergenic 30.0%
! CAAACATATAGCTTTTGTTG+GGG + chr5_3:23662260-23662279 Msa0806160:CDS 30.0%
AGTCCAATAAATTTCGTAAG+TGG + chr5_3:23662367-23662386 Msa0806160:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG + chr5_3:23662406-23662425 Msa0806160:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG + chr5_3:23662448-23662467 Msa0806160:CDS 30.0%
!! GACATGATTGTTTTATCAAC+TGG + chr5_3:23662537-23662556 Msa0806160:CDS 30.0%
TCACTCTCATAGTAAATTGA+TGG - chr5_3:23662579-23662598 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG + chr5_3:23662588-23662607 Msa0806160:CDS 30.0%
CCCTTAATAGTATAATTGAG+AGG + chr5_3:23662685-23662704 Msa0806160:CDS 30.0%
AGAAAAGAGGATAAGATAGT+TGG + chr5_3:23662816-23662835 Msa0806160:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG - chr5_3:23662984-23663003 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG + chr5_3:23662990-23663009 Msa0806160:CDS 30.0%
TACAAAACGATTGTGTTCAT+TGG + chr5_3:23663027-23663046 Msa0806160:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG - chr5_3:23663065-23663084 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG + chr5_3:23663087-23663106 Msa0806160:CDS 30.0%
TGTAGAGATGATGAAGAAAT+GGG + chr5_3:23663088-23663107 Msa0806160:CDS 30.0%
CTTTATGAGAGAGTCATGAA+AGG - chr5_3:23661490-23661509 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG + chr5_3:23661554-23661573 Msa0806160:five_prime_UTR 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG + chr5_3:23661566-23661585 Msa0806160:exon 35.0%
AAAGGGTTTGAAAATGGGAA+GGG + chr5_3:23661572-23661591 Msa0806160:exon 35.0%
AGGAAAGATTGTCATTGAAG+GGG - chr5_3:23661602-23661621 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG - chr5_3:23661603-23661622 None:intergenic 35.0%
GTAGAATGAAGAAGCTTAGT+TGG - chr5_3:23661723-23661742 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG - chr5_3:23661757-23661776 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG + chr5_3:23662017-23662036 Msa0806160:intron 35.0%
GTGACTATTCAAATGGAGAT+TGG + chr5_3:23662061-23662080 Msa0806160:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG - chr5_3:23662105-23662124 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG + chr5_3:23662105-23662124 Msa0806160:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG - chr5_3:23662302-23662321 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG - chr5_3:23662359-23662378 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG - chr5_3:23662373-23662392 None:intergenic 35.0%
GAAAAGGTAGCATTATGAGA+AGG - chr5_3:23662399-23662418 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG + chr5_3:23662447-23662466 Msa0806160:CDS 35.0%
GGCAAAGGATATTGATCAAA+TGG + chr5_3:23662515-23662534 Msa0806160:CDS 35.0%
!! TTGTTTTATCAACTGGGCAT+TGG + chr5_3:23662544-23662563 Msa0806160:CDS 35.0%
! TTCCTTTAAGAAAGGCTTTG+AGG + chr5_3:23662658-23662677 Msa0806160:CDS 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG + chr5_3:23662699-23662718 Msa0806160:CDS 35.0%
AGGAAAGTTGCTAAAGGAAA+TGG + chr5_3:23662705-23662724 Msa0806160:CDS 35.0%
! TTTTCACCTGCTCATTTTGA+AGG + chr5_3:23662750-23662769 Msa0806160:CDS 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG + chr5_3:23662758-23662777 Msa0806160:CDS 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG + chr5_3:23662764-23662783 Msa0806160:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG + chr5_3:23662803-23662822 Msa0806160:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG + chr5_3:23662819-23662838 Msa0806160:CDS 35.0%
AAGGATTGCTATAGAAGAAG+TGG + chr5_3:23662860-23662879 Msa0806160:CDS 35.0%
AGGTATTAGAATTGAGGTGT+TGG + chr5_3:23662914-23662933 Msa0806160:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG + chr5_3:23662929-23662948 Msa0806160:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG - chr5_3:23662996-23663015 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG - chr5_3:23663002-23663021 None:intergenic 35.0%
AGTAACATCCACATAGAGAA+GGG + chr5_3:23663111-23663130 Msa0806160:exon 35.0%
CTTTATGAGAGAGTCATGAA+AGG - chr5_3:23661490-23661509 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG + chr5_3:23661554-23661573 Msa0806160:five_prime_UTR 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG + chr5_3:23661566-23661585 Msa0806160:exon 35.0%
AAAGGGTTTGAAAATGGGAA+GGG + chr5_3:23661572-23661591 Msa0806160:exon 35.0%
AGGAAAGATTGTCATTGAAG+GGG - chr5_3:23661602-23661621 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG - chr5_3:23661603-23661622 None:intergenic 35.0%
GTAGAATGAAGAAGCTTAGT+TGG - chr5_3:23661723-23661742 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG - chr5_3:23661757-23661776 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG + chr5_3:23662017-23662036 Msa0806160:intron 35.0%
GTGACTATTCAAATGGAGAT+TGG + chr5_3:23662061-23662080 Msa0806160:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG - chr5_3:23662105-23662124 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG + chr5_3:23662105-23662124 Msa0806160:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG - chr5_3:23662302-23662321 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG - chr5_3:23662359-23662378 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG - chr5_3:23662373-23662392 None:intergenic 35.0%
GAAAAGGTAGCATTATGAGA+AGG - chr5_3:23662399-23662418 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG + chr5_3:23662447-23662466 Msa0806160:CDS 35.0%
GGCAAAGGATATTGATCAAA+TGG + chr5_3:23662515-23662534 Msa0806160:CDS 35.0%
!! TTGTTTTATCAACTGGGCAT+TGG + chr5_3:23662544-23662563 Msa0806160:CDS 35.0%
! TTCCTTTAAGAAAGGCTTTG+AGG + chr5_3:23662658-23662677 Msa0806160:CDS 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG + chr5_3:23662699-23662718 Msa0806160:CDS 35.0%
AGGAAAGTTGCTAAAGGAAA+TGG + chr5_3:23662705-23662724 Msa0806160:CDS 35.0%
! TTTTCACCTGCTCATTTTGA+AGG + chr5_3:23662750-23662769 Msa0806160:CDS 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG + chr5_3:23662758-23662777 Msa0806160:CDS 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG + chr5_3:23662764-23662783 Msa0806160:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG + chr5_3:23662803-23662822 Msa0806160:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG + chr5_3:23662819-23662838 Msa0806160:CDS 35.0%
AAGGATTGCTATAGAAGAAG+TGG + chr5_3:23662860-23662879 Msa0806160:CDS 35.0%
AGGTATTAGAATTGAGGTGT+TGG + chr5_3:23662914-23662933 Msa0806160:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG + chr5_3:23662929-23662948 Msa0806160:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG - chr5_3:23662996-23663015 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG - chr5_3:23663002-23663021 None:intergenic 35.0%
AGTAACATCCACATAGAGAA+GGG + chr5_3:23663111-23663130 Msa0806160:exon 35.0%
GAGTCATGAAAGGAAGAGAA+AGG - chr5_3:23661480-23661499 None:intergenic 40.0%
GTTGTTGTCGGCAAGAAAAA+CGG - chr5_3:23661516-23661535 None:intergenic 40.0%
GAAAGGGTTTGAAAATGGGA+AGG + chr5_3:23661571-23661590 Msa0806160:exon 40.0%
GGAGGAAAGATTGTCATTGA+AGG - chr5_3:23661604-23661623 None:intergenic 40.0%
CAAAAGGAGTAAGGGACAAA+AGG - chr5_3:23661698-23661717 None:intergenic 40.0%
! CTTTTGTCCCTTACTCCTTT+TGG + chr5_3:23661696-23661715 Msa0806160:CDS 40.0%
AGTTGGTCCAAAAGGAGTAA+GGG - chr5_3:23661706-23661725 None:intergenic 40.0%
TAGTTGGTCCAAAAGGAGTA+AGG - chr5_3:23661707-23661726 None:intergenic 40.0%
AGAAGCTTAGTTGGTCCAAA+AGG - chr5_3:23661714-23661733 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG - chr5_3:23661754-23661773 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG - chr5_3:23661786-23661805 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG + chr5_3:23661783-23661802 Msa0806160:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG - chr5_3:23662121-23662140 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG + chr5_3:23662141-23662160 Msa0806160:CDS 40.0%
GTGAGAGATGCATCACAAAT+GGG + chr5_3:23662142-23662161 Msa0806160:CDS 40.0%
ACAAATGGGAGACCTGATTT+AGG + chr5_3:23662156-23662175 Msa0806160:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG - chr5_3:23662204-23662223 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG + chr5_3:23662202-23662221 Msa0806160:CDS 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG - chr5_3:23662222-23662241 None:intergenic 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG - chr5_3:23662234-23662253 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG + chr5_3:23662269-23662288 Msa0806160:CDS 40.0%
ACTTGCCATCTTGGTAAACA+CGG - chr5_3:23662350-23662369 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG - chr5_3:23662415-23662434 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG - chr5_3:23662433-23662452 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG - chr5_3:23662434-23662453 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG - chr5_3:23662435-23662454 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG + chr5_3:23662493-23662512 Msa0806160:CDS 40.0%
GGATCTTGTCAATGAGAAGT+GGG + chr5_3:23662494-23662513 Msa0806160:CDS 40.0%
GTCCTCAAAGCCTTTCTTAA+AGG - chr5_3:23662663-23662682 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG - chr5_3:23662759-23662778 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG + chr5_3:23662757-23662776 Msa0806160:CDS 40.0%
GGATAAGATAGTTGGAGGAA+TGG + chr5_3:23662824-23662843 Msa0806160:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG + chr5_3:23662828-23662847 Msa0806160:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG + chr5_3:23662841-23662860 Msa0806160:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG + chr5_3:23662948-23662967 Msa0806160:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG - chr5_3:23662978-23662997 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG - chr5_3:23663059-23663078 None:intergenic 40.0%
GAGTAACATCCACATAGAGA+AGG + chr5_3:23663110-23663129 Msa0806160:exon 40.0%
GAGTCATGAAAGGAAGAGAA+AGG - chr5_3:23661480-23661499 None:intergenic 40.0%
GTTGTTGTCGGCAAGAAAAA+CGG - chr5_3:23661516-23661535 None:intergenic 40.0%
GAAAGGGTTTGAAAATGGGA+AGG + chr5_3:23661571-23661590 Msa0806160:exon 40.0%
GGAGGAAAGATTGTCATTGA+AGG - chr5_3:23661604-23661623 None:intergenic 40.0%
CAAAAGGAGTAAGGGACAAA+AGG - chr5_3:23661698-23661717 None:intergenic 40.0%
! CTTTTGTCCCTTACTCCTTT+TGG + chr5_3:23661696-23661715 Msa0806160:CDS 40.0%
AGTTGGTCCAAAAGGAGTAA+GGG - chr5_3:23661706-23661725 None:intergenic 40.0%
TAGTTGGTCCAAAAGGAGTA+AGG - chr5_3:23661707-23661726 None:intergenic 40.0%
AGAAGCTTAGTTGGTCCAAA+AGG - chr5_3:23661714-23661733 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG - chr5_3:23661754-23661773 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG - chr5_3:23661786-23661805 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG + chr5_3:23661783-23661802 Msa0806160:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG - chr5_3:23662121-23662140 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG + chr5_3:23662141-23662160 Msa0806160:CDS 40.0%
GTGAGAGATGCATCACAAAT+GGG + chr5_3:23662142-23662161 Msa0806160:CDS 40.0%
ACAAATGGGAGACCTGATTT+AGG + chr5_3:23662156-23662175 Msa0806160:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG - chr5_3:23662204-23662223 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG + chr5_3:23662202-23662221 Msa0806160:CDS 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG - chr5_3:23662222-23662241 None:intergenic 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG - chr5_3:23662234-23662253 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG + chr5_3:23662269-23662288 Msa0806160:CDS 40.0%
ACTTGCCATCTTGGTAAACA+CGG - chr5_3:23662350-23662369 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG - chr5_3:23662415-23662434 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG - chr5_3:23662433-23662452 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG - chr5_3:23662434-23662453 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG - chr5_3:23662435-23662454 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG + chr5_3:23662493-23662512 Msa0806160:CDS 40.0%
GGATCTTGTCAATGAGAAGT+GGG + chr5_3:23662494-23662513 Msa0806160:CDS 40.0%
GTCCTCAAAGCCTTTCTTAA+AGG - chr5_3:23662663-23662682 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG - chr5_3:23662759-23662778 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG + chr5_3:23662757-23662776 Msa0806160:CDS 40.0%
GGATAAGATAGTTGGAGGAA+TGG + chr5_3:23662824-23662843 Msa0806160:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG + chr5_3:23662828-23662847 Msa0806160:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG + chr5_3:23662841-23662860 Msa0806160:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG + chr5_3:23662948-23662967 Msa0806160:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG - chr5_3:23662978-23662997 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG - chr5_3:23663059-23663078 None:intergenic 40.0%
GAGTAACATCCACATAGAGA+AGG + chr5_3:23663110-23663129 Msa0806160:exon 40.0%
TGAATAGTGGTAGGTGGCAA+TGG - chr5_3:23661748-23661767 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG + chr5_3:23662078-23662097 Msa0806160:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG + chr5_3:23662274-23662293 Msa0806160:CDS 45.0%
CCATCTTGGTAAACACGGTT+AGG - chr5_3:23662345-23662364 None:intergenic 45.0%
CCTAACCGTGTTTACCAAGA+TGG + chr5_3:23662342-23662361 Msa0806160:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+AGG + chr5_3:23662500-23662519 Msa0806160:CDS 45.0%
TGCCAGGACCTATAGATACT+TGG + chr5_3:23663054-23663073 Msa0806160:CDS 45.0%
ATGCATCACCCTTCTCTATG+TGG - chr5_3:23663122-23663141 None:intergenic 45.0%
TGAATAGTGGTAGGTGGCAA+TGG - chr5_3:23661748-23661767 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG + chr5_3:23662078-23662097 Msa0806160:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG + chr5_3:23662274-23662293 Msa0806160:CDS 45.0%
CCATCTTGGTAAACACGGTT+AGG - chr5_3:23662345-23662364 None:intergenic 45.0%
CCTAACCGTGTTTACCAAGA+TGG + chr5_3:23662342-23662361 Msa0806160:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+AGG + chr5_3:23662500-23662519 Msa0806160:CDS 45.0%
TGCCAGGACCTATAGATACT+TGG + chr5_3:23663054-23663073 Msa0806160:CDS 45.0%
ATGCATCACCCTTCTCTATG+TGG - chr5_3:23663122-23663141 None:intergenic 45.0%
ATAGTGGTAGGTGGCAATGG+TGG - chr5_3:23661745-23661764 None:intergenic 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG + chr5_3:23662426-23662445 Msa0806160:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG + chr5_3:23662768-23662787 Msa0806160:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG + chr5_3:23662957-23662976 Msa0806160:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG - chr5_3:23662966-23662985 None:intergenic 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG + chr5_3:23663038-23663057 Msa0806160:CDS 50.0%
ATAGTGGTAGGTGGCAATGG+TGG - chr5_3:23661745-23661764 None:intergenic 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG + chr5_3:23662426-23662445 Msa0806160:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG + chr5_3:23662768-23662787 Msa0806160:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG + chr5_3:23662957-23662976 Msa0806160:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG - chr5_3:23662966-23662985 None:intergenic 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG + chr5_3:23663038-23663057 Msa0806160:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 23661446 23663164 23661446 ID=Msa0806160;Name=Msa0806160
chr5_3 mRNA 23661446 23663164 23661446 ID=Msa0806160-mRNA-1;Parent=Msa0806160;Name=Msa0806160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=139|1|1|1|1|1|2|49|435
chr5_3 exon 23661446 23661804 23661446 ID=Msa0806160-mRNA-1:exon:9286;Parent=Msa0806160-mRNA-1
chr5_3 exon 23662028 23663164 23662028 ID=Msa0806160-mRNA-1:exon:9287;Parent=Msa0806160-mRNA-1
chr5_3 five_prime_UTR 23661446 23661584 23661446 ID=Msa0806160-mRNA-1:five_prime_utr;Parent=Msa0806160-mRNA-1
chr5_3 CDS 23661585 23661804 23661585 ID=Msa0806160-mRNA-1:cds;Parent=Msa0806160-mRNA-1
chr5_3 CDS 23662028 23663115 23662028 ID=Msa0806160-mRNA-1:cds;Parent=Msa0806160-mRNA-1
chr5_3 three_prime_UTR 23663116 23663164 23663116 ID=Msa0806160-mRNA-1:three_prime_utr;Parent=Msa0806160-mRNA-1
Gene Sequence

>Msa0806160

ATGGGAAGGGCAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCCTTTTGTCCCTTACTCCTTTTGGACCAACTAAGCTTCTTCATTCTACCACCATTGCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGGAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGGTTTGAACCAAGCACTTTCCTCAATCTCATTAAGAACAAACATATAGCTTTTGTTGGGGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTATTGACTACTTCAACACCTAACCGTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAAGTGGGCAAAGGATATTGATCAAATGGACATGATTGTTTTATCAACTGGGCATTGGTTTTTGCTTCCATCAATTTACTATGAGAGTGATTTAGTTCTTGGTAGCTTGAATTGTCCTGAGTTTAATCATACTCAAATTGATTTTTATGTTCCTTTAAGAAAGGCTTTGAGGACTGCCCTTAATAGTATAATTGAGAGGAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGCTATAGAAGAAGTGGAAAGTGCCAAGACAAAAGCTAATAAATTTGAAGGTATTAGAATTGAGGTGTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAACGATTGTGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTCTTGTAGAGATGATGAAGAAATGGGAGTAA
Protein sequence

>Msa0806160

MGRANPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFILLSLTPFGPTKLLHSTTIATYHYSSTYSSPPSTSFVGKNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPSTFLNLIKNKHIAFVGDSLARNQLESLLCMLLTTSTPNRVYQDGKSNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNEKWAKDIDQMDMIVLSTGHWFLLPSIYYESDLVLGSLNCPEFNHTQIDFYVPLRKALRTALNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIAIEEVESAKTKANKFEGIRIEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEILVEMMKKWE*