AlfalfaGEDB | Msa0815080

M. sativa cultivar zhongmu-4 / Msa0815080

KEGG: K10706
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815080	AES99078.2	80.597	67	13	0	3	69	814	880	4.33e-29	119

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815080	G7KFP1	80.597	67	13	0	3	69	814	880	2.07e-29	119

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0736000	Msa0815080	0.849877	2.438615e-60	-8.615850e-47
Msa0777790	Msa0815080	0.876708	1.126260e-68	-8.615850e-47
Msa0777850	Msa0815080	0.872491	3.054287e-67	-8.615850e-47
Msa0814940	Msa0815080	0.818234	2.179923e-52	-8.615850e-47
Msa0815080	Msa0815140	0.943880	5.409097e-103	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815080	MtrunA17_Chr5g0433361	80.597	67	13	3	69	814	880	3.99e-33	119
Msa0815080	MtrunA17_Chr5g0433331	79.688	64	13	3	66	86	149	1.02e-32	110
Msa0815080	MtrunA17_Chr5g0433421	79.688	64	13	3	66	928	991	1.32e-30	111
Msa0815080	MtrunA17_Chr5g0433511	76.562	64	15	3	66	843	906	2.17e-25	97.1
Msa0815080	MtrunA17_Chr3g0109541	64.062	64	23	3	66	844	907	1.94e-24	94.4
Msa0815080	MtrunA17_Chr5g0433321	62.687	67	25	3	69	820	886	3.87e-24	93.6
Msa0815080	MtrunA17_Chr5g0433371	59.701	67	27	3	69	844	910	1.95e-23	91.3
Msa0815080	MtrunA17_Chr5g0433471	71.875	64	18	3	66	843	906	2.66e-23	90.9
Msa0815080	MtrunA17_Chr3g0109551	43.103	58	33	3	60	879	936	1.88e-14	65.9
Msa0815080	MtrunA17_Chr8g0375151	48.276	58	30	3	60	787	844	2.15e-14	65.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815080	AT1G65780.1	50.000	70	29	1	3	66	807	876	5.28e-17	73.6
Msa0815080	AT1G65810.2	67.391	46	15	0	3	48	792	837	2.71e-16	71.6
Msa0815080	AT1G65810.3	67.391	46	15	0	3	48	792	837	3.05e-16	71.2
Msa0815080	AT1G65810.1	67.391	46	15	0	3	48	792	837	3.05e-16	71.2
Msa0815080	AT5G37030.1	47.917	48	25	0	3	50	589	636	8.04e-12	58.9

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Find 11 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAGAACATTCTTAATAGCC+CGG	0.537690	5_3:+47998926	None:intergenic
AGATACTGTCTTTGGATATT+AGG	0.305787	5_3:-47999054	Msa0815080:CDS
AGTTATTGATGCTAAGAATA+GGG	0.406528	5_3:-47998986	Msa0815080:CDS
ATTCTTCCAGTCGTTGAATC+TGG	0.301086	5_3:+47998902	None:intergenic
CAGAACATTCTTAATAGCCC+GGG	0.595796	5_3:+47998927	None:intergenic
CCTAAACGCCGTTTCTTGAA+TGG	0.370619	5_3:+47998871	None:intergenic
TAATCAACAGTGACTCTGTT+TGG	0.369827	5_3:-47999017	Msa0815080:CDS
TAGTTATTGATGCTAAGAAT+AGG	0.429708	5_3:-47998987	Msa0815080:CDS
TCTGTTCCAGATTCAACGAC+TGG	0.536252	5_3:-47998908	Msa0815080:CDS
TTGAGTCACCATTCAAGAAA+CGG	0.508950	5_3:-47998879	Msa0815080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATACTGTCTTTGGATATT+AGG	-	chr5_3:47998520-47998539	Msa0815080:intron	30.0%
ACATATCAACTACTTTCTCT+AGG	-	chr5_3:47998746-47998765	Msa0815080:intron	30.0%
TGGTTAATCAAGTTTCATGT+AGG	-	chr5_3:47998910-47998929	Msa0815080:CDS	30.0%
GGTTAATCAAGTTTCATGTA+GGG	-	chr5_3:47998911-47998930	Msa0815080:CDS	30.0%
TAATCAACAGTGACTCTGTT+TGG	-	chr5_3:47998557-47998576	Msa0815080:intron	35.0%
TGCTGAAGAGGACAAAAAAT+TGG	-	chr5_3:47998624-47998643	Msa0815080:intron	35.0%
ACAGAACATTCTTAATAGCC+CGG	+	chr5_3:47998651-47998670	None:intergenic	35.0%
TTGAGTCACCATTCAAGAAA+CGG	-	chr5_3:47998695-47998714	Msa0815080:intron	35.0%
TAATCAAGTTTCATGTAGGG+TGG	-	chr5_3:47998914-47998933	Msa0815080:CDS	35.0%
AATCAAGTTTCATGTAGGGT+GGG	-	chr5_3:47998915-47998934	Msa0815080:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	47998452	47999144	47998452	ID=Msa0815080;Name=Msa0815080
chr5_3	mRNA	47998452	47999144	47998452	ID=Msa0815080-mRNA-1;Parent=Msa0815080;Name=Msa0815080-mRNA-1;_AED=0.51;_eAED=0.52;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|69
chr5_3	exon	47999140	47999144	47999140	ID=Msa0815080-mRNA-1:exon:14571;Parent=Msa0815080-mRNA-1
chr5_3	exon	47998878	47999074	47998878	ID=Msa0815080-mRNA-1:exon:14570;Parent=Msa0815080-mRNA-1
chr5_3	exon	47998452	47998459	47998452	ID=Msa0815080-mRNA-1:exon:14569;Parent=Msa0815080-mRNA-1
chr5_3	CDS	47999140	47999144	47999140	ID=Msa0815080-mRNA-1:cds;Parent=Msa0815080-mRNA-1
chr5_3	CDS	47998878	47999074	47998878	ID=Msa0815080-mRNA-1:cds;Parent=Msa0815080-mRNA-1
chr5_3	CDS	47998452	47998459	47998452	ID=Msa0815080-mRNA-1:cds;Parent=Msa0815080-mRNA-1

Gene Sequence	>Msa0815080 ATGACATACTGTCTTTGGATATTAGGAAATGCATCTACTTTAATCAACAGTGACTCTGTTTGGAGAAATGTAGTTATTGATGCTAAGAATAGGGATTGTTTCCACAATGCTGAAGAGGACAAAAAATTGGCCCGGGCTATTAAGAATGTTCTGTTCCAGATTCAACGACTGGAAGAATTTGAGTCACCATTCAAGAAACGGCATATTTGA
Protein sequence	>Msa0815080 MTYCLWILGNASTLINSDSVWRNVVIDAKNRDCFHNAEEDKKLARAIKNVLFQIQRLEEFESPFKKRHI*