AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215580 MtrunA17_Chr4g0064691 97.041 169 5 0 1 169 1 169 3.01e-109 308
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTTCTTTGCTGGTGGC+TGG 0.201684 8_1:+76866841 None:intergenic
TCTCACTTCCTCTTCTTTGC+TGG 0.250916 8_1:+76866834 None:intergenic
TTCCCTTTGGAGCACCTTCT+TGG 0.276978 8_1:-76867143 Msa1215580:CDS
CCTCTTCTTTGCTGGTGGCT+GGG 0.297534 8_1:+76866842 None:intergenic
TTGTGTCAAGCTTAGTGATT+AGG 0.324884 8_1:-76866775 Msa1215580:three_prime_UTR
CTCTTCTTTGCTGGTGGCTG+GGG 0.325642 8_1:+76866843 None:intergenic
GAATTATATTAGTAGGAAAT+TGG 0.326206 8_1:-76866813 Msa1215580:three_prime_UTR
CAACTTTGGAGCCACTGATT+TGG 0.351405 8_1:+76867266 None:intergenic
ATTGCTTGTGCTATCAATCT+TGG 0.352766 8_1:-76866593 Msa1215580:three_prime_UTR
AGAGAATTGGAAGGTGAGAT+TGG 0.354776 8_1:+76868304 None:intergenic
CACTCACGGACAATAATAAC+TGG 0.374970 8_1:+76866647 None:intergenic
AATCCTTGTCAGGTTCCCTT+TGG 0.384520 8_1:-76867156 Msa1215580:intron
TGACACAGGTTCACTTACTA+AGG 0.409062 8_1:+76866736 None:intergenic
ACTTGAGTTGAGTTGAGTCC+AGG 0.410529 8_1:-76868419 Msa1215580:five_prime_UTR
GAATCCATTGCAGTCAACTT+TGG 0.411321 8_1:+76867252 None:intergenic
CACTTCCTCTTCTTTGCTGG+TGG 0.417470 8_1:+76866837 None:intergenic
TTCTTCCTCGCCTTCATCAC+CGG 0.419016 8_1:+76867010 None:intergenic
GAGGAGTCAACAACAACAAT+AGG 0.424929 8_1:+76868357 None:intergenic
AGAGGACGAAGAGGAGGAAG+AGG 0.432417 8_1:-76866880 Msa1215580:CDS
TTAGTAGGAAATTGGTTATG+TGG 0.455585 8_1:-76866805 Msa1215580:three_prime_UTR
AGGTCGACGACGGAGAGAAT+TGG 0.458122 8_1:+76868291 None:intergenic
TGAGGATGATGATGGAGAAG+AGG 0.459842 8_1:-76867042 Msa1215580:CDS
GAAGTGAGAATTATATTAGT+AGG 0.468566 8_1:-76866820 Msa1215580:exon
GATCCTGAGGCTAACGGTGC+AGG 0.476679 8_1:-76866972 Msa1215580:CDS
GTCAACAACAACAATAGGTT+TGG 0.480887 8_1:+76868362 None:intergenic
GGTGATGAAGGCGAGGAAGA+AGG 0.491443 8_1:-76867008 Msa1215580:CDS
GAGAATTGGAAGGTGAGATT+GGG 0.491676 8_1:+76868305 None:intergenic
GAAGACTACTCCGGTGATGA+AGG 0.492863 8_1:-76867020 Msa1215580:CDS
CTTTGGAGCACCTTCTTGGA+AGG 0.496704 8_1:-76867139 Msa1215580:CDS
CTCTCTCTCACTCTCGGCAA+TGG 0.497209 8_1:-76868337 Msa1215580:exon
GGTGCAGGAGGAAGTGATGA+TGG 0.499986 8_1:-76866957 Msa1215580:CDS
GTGATGAAGGCGAGGAAGAA+GGG 0.503531 8_1:-76867007 Msa1215580:CDS
CTTCCAAGAAGGTGCTCCAA+AGG 0.509009 8_1:+76867140 None:intergenic
CCTCCTGCACCGTTAGCCTC+AGG 0.511442 8_1:+76866969 None:intergenic
CCCAGCCACCAGCAAAGAAG+AGG 0.521663 8_1:-76866842 Msa1215580:CDS
TTCCAAGAAGGTGCTCCAAA+GGG 0.531221 8_1:+76867141 None:intergenic
GAGTGTCGACGGCGACAGCT+TGG 0.539506 8_1:+76868256 None:intergenic
GTGCAGGAGGAAGTGATGAT+GGG 0.542524 8_1:-76866956 Msa1215580:CDS
CTTGAGTTGAGTTGAGTCCA+GGG 0.545804 8_1:-76868418 Msa1215580:five_prime_UTR
GGCTCCAAAGTTGACTGCAA+TGG 0.554467 8_1:-76867256 Msa1215580:CDS
AGTTCCACAAAACCCTAAAA+CGG 0.558071 8_1:-76868448 Msa1215580:five_prime_UTR
TGACTCCTCTCTCTCACTCT+CGG 0.562392 8_1:-76868343 Msa1215580:five_prime_UTR
TTAGCCTCAGGATCATCCTC+GGG 0.566086 8_1:+76866981 None:intergenic
GCGACAGCTTGGAGAAGAGA+GGG 0.568266 8_1:+76868267 None:intergenic
CATTGCCGAGAGTGAGAGAG+AGG 0.573058 8_1:+76868338 None:intergenic
AAGCTGGCTTCCTTCCAAGA+AGG 0.576452 8_1:+76867129 None:intergenic
AAGAGAGGGGAGGTCGACGA+CGG 0.580788 8_1:+76868281 None:intergenic
AGATGAAGAGGACGAAGAGG+AGG 0.581319 8_1:-76866886 Msa1215580:CDS
GGCGACAGCTTGGAGAAGAG+AGG 0.582331 8_1:+76868266 None:intergenic
GATGAGGAAGATGAAGAAGA+TGG 0.583508 8_1:-76866912 Msa1215580:CDS
GTTAGCCTCAGGATCATCCT+CGG 0.587042 8_1:+76866980 None:intergenic
TGATGAAGGCGAGGAAGAAG+GGG 0.595555 8_1:-76867006 Msa1215580:CDS
CCTGAGGCTAACGGTGCAGG+AGG 0.603799 8_1:-76866969 Msa1215580:CDS
CGAAGATGAAGAGGACGAAG+AGG 0.605829 8_1:-76866889 Msa1215580:CDS
AGAAGATGGCGAAGATGAAG+AGG 0.607145 8_1:-76866898 Msa1215580:CDS
TCCACAAAACCCTAAAACGG+TGG 0.610189 8_1:-76868445 Msa1215580:five_prime_UTR
GGCGGAAACAAGAGTGTCGA+CGG 0.610291 8_1:+76868245 None:intergenic
TGATTAGAGCGAGTAAACCC+TGG 0.612107 8_1:+76868401 None:intergenic
GTACAAGATGAGGATGATGA+TGG 0.612789 8_1:-76867050 Msa1215580:CDS
AAGTGATACAGAAGATGATG+AGG 0.617223 8_1:-76867093 Msa1215580:CDS
CGAGGAAGAAGGGGATCCCG+AGG 0.625175 8_1:-76866997 Msa1215580:CDS
GGAGAAGAGGAAGACTACTC+CGG 0.625811 8_1:-76867029 Msa1215580:CDS
GACTAATGCTGCCAAATCAG+TGG 0.634709 8_1:-76867277 Msa1215580:CDS
CTACTCCGGTGATGAAGGCG+AGG 0.636930 8_1:-76867015 Msa1215580:CDS
TGCAGGAGGAAGTGATGATG+GGG 0.639993 8_1:-76866955 Msa1215580:CDS
TACGAGAGCGAGAGATTGAG+CGG 0.643982 8_1:+76868224 None:intergenic
GAGGATGATCCTGAGGCTAA+CGG 0.649850 8_1:-76866978 Msa1215580:CDS
CGACGACGGAGAGAATTGGA+AGG 0.661376 8_1:+76868295 None:intergenic
TCATTGACATAACTTGACAC+AGG 0.664341 8_1:+76866722 None:intergenic
CGACAGCTTGGAGAAGAGAG+GGG 0.672671 8_1:+76868268 None:intergenic
GGATCCCGAGGATGATCCTG+AGG 0.672779 8_1:-76866985 Msa1215580:CDS
CAGCTTGGAGAAGAGAGGGG+AGG 0.680758 8_1:+76868271 None:intergenic
TGATGATGACGTACAAGATG+AGG 0.687848 8_1:-76867060 Msa1215580:CDS
TGATGATGACGATGGCGATG+AGG 0.692718 8_1:-76866928 Msa1215580:CDS
GGGGAAGATGATGATGACGA+TGG 0.701589 8_1:-76866936 Msa1215580:CDS
GAGAGCGAGAGATTGAGCGG+CGG 0.738155 8_1:+76868227 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGCATAGTAAAATTAAAA+GGG + chr8_1:76866850-76866869 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + chr8_1:76866851-76866870 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - chr8_1:76867182-76867201 Msa1215580:intron 15.0%
!! AATGCATAGTAAAATTAAAA+GGG + chr8_1:76866850-76866869 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + chr8_1:76866851-76866870 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - chr8_1:76867182-76867201 Msa1215580:intron 15.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + chr8_1:76866837-76866856 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + chr8_1:76866838-76866857 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + chr8_1:76866970-76866989 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - chr8_1:76867631-76867650 Msa1215580:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - chr8_1:76867658-76867677 Msa1215580:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - chr8_1:76868198-76868217 Msa1215580:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - chr8_1:76868383-76868402 Msa1215580:five_prime_UTR 20.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + chr8_1:76866837-76866856 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + chr8_1:76866838-76866857 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + chr8_1:76866970-76866989 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - chr8_1:76867631-76867650 Msa1215580:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - chr8_1:76867658-76867677 Msa1215580:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - chr8_1:76868198-76868217 Msa1215580:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - chr8_1:76868383-76868402 Msa1215580:five_prime_UTR 20.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + chr8_1:76866836-76866855 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - chr8_1:76867131-76867150 Msa1215580:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - chr8_1:76867166-76867185 Msa1215580:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - chr8_1:76868191-76868210 Msa1215580:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + chr8_1:76868381-76868400 None:intergenic 25.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + chr8_1:76866836-76866855 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - chr8_1:76867131-76867150 Msa1215580:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - chr8_1:76867166-76867185 Msa1215580:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - chr8_1:76868191-76868210 Msa1215580:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + chr8_1:76868381-76868400 None:intergenic 25.0%
TAAGCATGTTGTGGAAATTA+GGG - chr8_1:76866887-76866906 Msa1215580:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + chr8_1:76866994-76867013 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - chr8_1:76867159-76867178 Msa1215580:intron 30.0%
AATATACAAGCATGCACATA+GGG + chr8_1:76867228-76867247 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - chr8_1:76867313-76867332 Msa1215580:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - chr8_1:76867421-76867440 Msa1215580:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + chr8_1:76867511-76867530 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + chr8_1:76867651-76867670 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + chr8_1:76867901-76867920 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - chr8_1:76868206-76868225 Msa1215580:intron 30.0%
TAAGCATGTTGTGGAAATTA+GGG - chr8_1:76866887-76866906 Msa1215580:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + chr8_1:76866994-76867013 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - chr8_1:76867159-76867178 Msa1215580:intron 30.0%
AATATACAAGCATGCACATA+GGG + chr8_1:76867228-76867247 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - chr8_1:76867313-76867332 Msa1215580:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - chr8_1:76867421-76867440 Msa1215580:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + chr8_1:76867511-76867530 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + chr8_1:76867651-76867670 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + chr8_1:76867901-76867920 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - chr8_1:76868206-76868225 Msa1215580:intron 30.0%
AGTTCCACAAAACCCTAAAA+CGG - chr8_1:76866563-76866582 Msa1215580:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + chr8_1:76866652-76866671 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + chr8_1:76866877-76866896 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - chr8_1:76866878-76866897 Msa1215580:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - chr8_1:76866886-76866905 Msa1215580:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + chr8_1:76866942-76866961 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - chr8_1:76866942-76866961 Msa1215580:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + chr8_1:76866957-76866976 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + chr8_1:76867077-76867096 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - chr8_1:76867135-76867154 Msa1215580:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + chr8_1:76867229-76867248 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - chr8_1:76867233-76867252 Msa1215580:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - chr8_1:76867321-76867340 Msa1215580:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + chr8_1:76867427-76867446 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + chr8_1:76867478-76867497 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - chr8_1:76867578-76867597 Msa1215580:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - chr8_1:76867587-76867606 Msa1215580:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - chr8_1:76867798-76867817 Msa1215580:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - chr8_1:76867845-76867864 Msa1215580:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + chr8_1:76867850-76867869 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - chr8_1:76867918-76867937 Msa1215580:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - chr8_1:76868236-76868255 Msa1215580:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + chr8_1:76868292-76868311 None:intergenic 35.0%
ATTGCTTGTGCTATCAATCT+TGG - chr8_1:76868418-76868437 Msa1215580:five_prime_UTR 35.0%
AGTTCCACAAAACCCTAAAA+CGG - chr8_1:76866563-76866582 Msa1215580:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + chr8_1:76866652-76866671 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + chr8_1:76866877-76866896 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - chr8_1:76866878-76866897 Msa1215580:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - chr8_1:76866886-76866905 Msa1215580:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + chr8_1:76866942-76866961 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - chr8_1:76866942-76866961 Msa1215580:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + chr8_1:76866957-76866976 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + chr8_1:76867077-76867096 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - chr8_1:76867135-76867154 Msa1215580:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + chr8_1:76867229-76867248 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - chr8_1:76867233-76867252 Msa1215580:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - chr8_1:76867321-76867340 Msa1215580:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + chr8_1:76867427-76867446 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + chr8_1:76867478-76867497 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - chr8_1:76867578-76867597 Msa1215580:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - chr8_1:76867587-76867606 Msa1215580:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - chr8_1:76867798-76867817 Msa1215580:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - chr8_1:76867845-76867864 Msa1215580:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + chr8_1:76867850-76867869 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - chr8_1:76867918-76867937 Msa1215580:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - chr8_1:76868236-76868255 Msa1215580:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + chr8_1:76868292-76868311 None:intergenic 35.0%
ATTGCTTGTGCTATCAATCT+TGG - chr8_1:76868418-76868437 Msa1215580:five_prime_UTR 35.0%
! TCAAGTACTCCACCGTTTTA+GGG + chr8_1:76866578-76866597 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + chr8_1:76866657-76866676 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + chr8_1:76866709-76866728 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + chr8_1:76866710-76866729 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + chr8_1:76867078-76867097 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + chr8_1:76867504-76867523 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + chr8_1:76867505-76867524 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - chr8_1:76867702-76867721 Msa1215580:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + chr8_1:76867718-76867737 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + chr8_1:76867719-76867738 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + chr8_1:76867762-76867781 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - chr8_1:76867951-76867970 Msa1215580:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - chr8_1:76867961-76867980 Msa1215580:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - chr8_1:76868099-76868118 Msa1215580:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + chr8_1:76868278-76868297 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + chr8_1:76868367-76868386 None:intergenic 40.0%
! TCAAGTACTCCACCGTTTTA+GGG + chr8_1:76866578-76866597 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + chr8_1:76866657-76866676 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + chr8_1:76866709-76866728 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + chr8_1:76866710-76866729 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + chr8_1:76867078-76867097 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + chr8_1:76867504-76867523 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + chr8_1:76867505-76867524 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - chr8_1:76867702-76867721 Msa1215580:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + chr8_1:76867718-76867737 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + chr8_1:76867719-76867738 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + chr8_1:76867762-76867781 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - chr8_1:76867951-76867970 Msa1215580:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - chr8_1:76867961-76867980 Msa1215580:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - chr8_1:76868099-76868118 Msa1215580:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + chr8_1:76868278-76868297 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + chr8_1:76868367-76868386 None:intergenic 40.0%
TCCACAAAACCCTAAAACGG+TGG - chr8_1:76866566-76866585 Msa1215580:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + chr8_1:76866570-76866589 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + chr8_1:76866579-76866598 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - chr8_1:76866592-76866611 Msa1215580:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - chr8_1:76866593-76866612 Msa1215580:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + chr8_1:76866613-76866632 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + chr8_1:76866928-76866947 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - chr8_1:76867140-76867159 Msa1215580:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - chr8_1:76867330-76867349 Msa1215580:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + chr8_1:76867717-76867736 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - chr8_1:76867734-76867753 Msa1215580:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + chr8_1:76867748-76867767 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - chr8_1:76867855-76867874 Msa1215580:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + chr8_1:76867873-76867892 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - chr8_1:76867969-76867988 Msa1215580:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - chr8_1:76868113-76868132 Msa1215580:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + chr8_1:76868180-76868199 None:intergenic 45.0%
TCCACAAAACCCTAAAACGG+TGG - chr8_1:76866566-76866585 Msa1215580:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + chr8_1:76866570-76866589 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + chr8_1:76866579-76866598 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - chr8_1:76866592-76866611 Msa1215580:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - chr8_1:76866593-76866612 Msa1215580:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + chr8_1:76866613-76866632 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + chr8_1:76866928-76866947 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - chr8_1:76867140-76867159 Msa1215580:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - chr8_1:76867330-76867349 Msa1215580:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + chr8_1:76867717-76867736 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - chr8_1:76867734-76867753 Msa1215580:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + chr8_1:76867748-76867767 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - chr8_1:76867855-76867874 Msa1215580:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + chr8_1:76867873-76867892 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - chr8_1:76867969-76867988 Msa1215580:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - chr8_1:76868113-76868132 Msa1215580:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + chr8_1:76868180-76868199 None:intergenic 45.0%
TGACTCCTCTCTCTCACTCT+CGG - chr8_1:76866668-76866687 Msa1215580:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + chr8_1:76866790-76866809 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - chr8_1:76867146-76867165 Msa1215580:CDS 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - chr8_1:76867755-76867774 Msa1215580:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - chr8_1:76867868-76867887 Msa1215580:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + chr8_1:76867874-76867893 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - chr8_1:76867872-76867891 Msa1215580:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + chr8_1:76867885-76867904 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - chr8_1:76867982-76868001 Msa1215580:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - chr8_1:76867991-76868010 Msa1215580:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + chr8_1:76868004-76868023 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - chr8_1:76868004-76868023 Msa1215580:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - chr8_1:76868005-76868024 Msa1215580:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + chr8_1:76868033-76868052 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + chr8_1:76868034-76868053 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - chr8_1:76868033-76868052 Msa1215580:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - chr8_1:76868055-76868074 Msa1215580:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - chr8_1:76868056-76868075 Msa1215580:intron 50.0%
! GGGGAAGATGATGATGACGA+TGG - chr8_1:76868075-76868094 Msa1215580:intron 50.0%
! TGATGATGACGATGGCGATG+AGG - chr8_1:76868083-76868102 Msa1215580:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - chr8_1:76868122-76868141 Msa1215580:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - chr8_1:76868125-76868144 Msa1215580:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + chr8_1:76868177-76868196 None:intergenic 50.0%
TGACTCCTCTCTCTCACTCT+CGG - chr8_1:76866668-76866687 Msa1215580:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + chr8_1:76866790-76866809 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - chr8_1:76867146-76867165 Msa1215580:CDS 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - chr8_1:76867755-76867774 Msa1215580:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - chr8_1:76867868-76867887 Msa1215580:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + chr8_1:76867874-76867893 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - chr8_1:76867872-76867891 Msa1215580:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + chr8_1:76867885-76867904 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - chr8_1:76867982-76868001 Msa1215580:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - chr8_1:76867991-76868010 Msa1215580:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + chr8_1:76868004-76868023 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - chr8_1:76868004-76868023 Msa1215580:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - chr8_1:76868005-76868024 Msa1215580:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + chr8_1:76868033-76868052 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + chr8_1:76868034-76868053 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - chr8_1:76868033-76868052 Msa1215580:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - chr8_1:76868055-76868074 Msa1215580:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - chr8_1:76868056-76868075 Msa1215580:intron 50.0%
! GGGGAAGATGATGATGACGA+TGG - chr8_1:76868075-76868094 Msa1215580:intron 50.0%
! TGATGATGACGATGGCGATG+AGG - chr8_1:76868083-76868102 Msa1215580:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - chr8_1:76868122-76868141 Msa1215580:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - chr8_1:76868125-76868144 Msa1215580:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + chr8_1:76868177-76868196 None:intergenic 50.0%
CATTGCCGAGAGTGAGAGAG+AGG + chr8_1:76866676-76866695 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - chr8_1:76866674-76866693 Msa1215580:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + chr8_1:76866719-76866738 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + chr8_1:76866723-76866742 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + chr8_1:76866746-76866765 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + chr8_1:76866747-76866766 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + chr8_1:76866769-76866788 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + chr8_1:76867151-76867170 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + chr8_1:76867861-76867880 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - chr8_1:76868003-76868022 Msa1215580:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - chr8_1:76868054-76868073 Msa1215580:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - chr8_1:76868131-76868150 Msa1215580:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + chr8_1:76868171-76868190 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + chr8_1:76868172-76868191 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + chr8_1:76868173-76868192 None:intergenic 55.0%
CATTGCCGAGAGTGAGAGAG+AGG + chr8_1:76866676-76866695 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - chr8_1:76866674-76866693 Msa1215580:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + chr8_1:76866719-76866738 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + chr8_1:76866723-76866742 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + chr8_1:76866746-76866765 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + chr8_1:76866747-76866766 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + chr8_1:76866769-76866788 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + chr8_1:76867151-76867170 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + chr8_1:76867861-76867880 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - chr8_1:76868003-76868022 Msa1215580:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - chr8_1:76868054-76868073 Msa1215580:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - chr8_1:76868131-76868150 Msa1215580:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + chr8_1:76868171-76868190 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + chr8_1:76868172-76868191 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + chr8_1:76868173-76868192 None:intergenic 55.0%
AAGAGAGGGGAGGTCGACGA+CGG + chr8_1:76866733-76866752 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + chr8_1:76866743-76866762 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + chr8_1:76866748-76866767 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + chr8_1:76866787-76866806 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - chr8_1:76867996-76868015 Msa1215580:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - chr8_1:76868026-76868045 Msa1215580:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - chr8_1:76868039-76868058 Msa1215580:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - chr8_1:76868169-76868188 Msa1215580:intron 60.0%
AAGAGAGGGGAGGTCGACGA+CGG + chr8_1:76866733-76866752 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + chr8_1:76866743-76866762 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + chr8_1:76866748-76866767 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + chr8_1:76866787-76866806 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - chr8_1:76867996-76868015 Msa1215580:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - chr8_1:76868026-76868045 Msa1215580:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - chr8_1:76868039-76868058 Msa1215580:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - chr8_1:76868169-76868188 Msa1215580:intron 60.0%
!! GAGTGTCGACGGCGACAGCT+TGG + chr8_1:76866758-76866777 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - chr8_1:76868014-76868033 Msa1215580:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + chr8_1:76868045-76868064 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - chr8_1:76868042-76868061 Msa1215580:intron 65.0%
!! GAGTGTCGACGGCGACAGCT+TGG + chr8_1:76866758-76866777 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - chr8_1:76868014-76868033 Msa1215580:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + chr8_1:76868045-76868064 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - chr8_1:76868042-76868061 Msa1215580:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 76866560 76868473 76866560 ID=Msa1215580;Name=Msa1215580
chr8_1 mRNA 76866560 76868473 76866560 ID=Msa1215580-mRNA-1;Parent=Msa1215580;Name=Msa1215580-mRNA-1;_AED=0.00;_eAED=0.00;_QI=133|1|1|1|1|1|3|276|169
chr8_1 exon 76866560 76867166 76866560 ID=Msa1215580-mRNA-1:exon:26454;Parent=Msa1215580-mRNA-1
chr8_1 exon 76867249 76867313 76867249 ID=Msa1215580-mRNA-1:exon:26453;Parent=Msa1215580-mRNA-1
chr8_1 exon 76868227 76868473 76868227 ID=Msa1215580-mRNA-1:exon:26452;Parent=Msa1215580-mRNA-1
chr8_1 five_prime_UTR 76868341 76868473 76868341 ID=Msa1215580-mRNA-1:five_prime_utr;Parent=Msa1215580-mRNA-1
chr8_1 CDS 76868227 76868340 76868227 ID=Msa1215580-mRNA-1:cds;Parent=Msa1215580-mRNA-1
chr8_1 CDS 76867249 76867313 76867249 ID=Msa1215580-mRNA-1:cds;Parent=Msa1215580-mRNA-1
chr8_1 CDS 76866836 76867166 76866836 ID=Msa1215580-mRNA-1:cds;Parent=Msa1215580-mRNA-1
chr8_1 three_prime_UTR 76866560 76866835 76866560 ID=Msa1215580-mRNA-1:three_prime_utr;Parent=Msa1215580-mRNA-1
Gene Sequence

>Msa1215580

ATGGCTGTGTCAACCCAATCTCACCTTCCAATTCTCTCCGTCGTCGACCTCCCCTCTCTTCTCCAAGCTGTCGCCGTCGACACTCTTGTTTCCGCCGCTCAATCTCTCGCTCTCATTGGATATCTGCTGACTAATGCTGCCAAATCAGTGGCTCCAAAGTTGACTGCAATGGATTCAATGTTCCCTTTGGAGCACCTTCTTGGAAGGAAGCCAGCTTACAAAGAAAACAAAAGTGATACAGAAGATGATGAGGACGACGACGATGATGATGACGTACAAGATGAGGATGATGATGGAGAAGAGGAAGACTACTCCGGTGATGAAGGCGAGGAAGAAGGGGATCCCGAGGATGATCCTGAGGCTAACGGTGCAGGAGGAAGTGATGATGGGGAAGATGATGATGACGATGGCGATGAGGAAGATGAAGAAGATGGCGAAGATGAAGAGGACGAAGAGGAGGAAGAGGAAGATGATGAGACTCCCCAGCCACCAGCAAAGAAGAGGAAGTGA
Protein sequence

>Msa1215580

MAVSTQSHLPILSVVDLPSLLQAVAVDTLVSAAQSLALIGYLLTNAAKSVAPKLTAMDSMFPLEHLLGRKPAYKENKSDTEDDEDDDDDDDVQDEDDDGEEEDYSGDEGEEEGDPEDDPEANGAGGSDDGEDDDDDGDEEDEEDGEDEEDEEEEEEDDETPQPPAKKRK*