AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0825180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825180 MtrunA17_Chr5g0446771 96.865 319 7 1 1 316 1 319 0.0 630
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825180 AT4G37000.1 53.571 280 125 2 40 316 41 318 3.10e-102 303

Find 64 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACGACCTTGCTTCTTC+TGG 0.137652 5_3:+64908703 None:intergenic
GTTTGAGATACAAATTTATT+TGG 0.161065 5_3:-64906733 Msa0825180:exon
ATCAGCTCGAACACGAAATT+TGG 0.213505 5_3:+64907237 None:intergenic
CGAAGCAAGTGTTGGGGATA+TGG 0.251288 5_3:-64906936 Msa0825180:CDS
TTCTGTTTCTTGTTCTATAA+TGG 0.268979 5_3:-64908730 Msa0825180:CDS
CTTCTTATTCTTCTTCTTCA+AGG 0.276636 5_3:-64908776 Msa0825180:CDS
CCCAACACTTGCTTCGAAAT+CGG 0.315726 5_3:+64906943 None:intergenic
GCTTCGAAATCGGGTCAATA+TGG 0.335220 5_3:+64906953 None:intergenic
AGAACAAGAATTAGCATTGT+TGG 0.348949 5_3:+64907204 None:intergenic
TCAAGCTTTCCAAGGTTGTT+TGG 0.358482 5_3:-64906805 Msa0825180:CDS
AACAGTAAAATACTCCTTGA+TGG 0.363131 5_3:+64906753 None:intergenic
GCTACGACCTTGCTTCTTCT+GGG 0.370091 5_3:+64908704 None:intergenic
AAGACTATTGAGGTTGATCT+TGG 0.379733 5_3:-64906829 Msa0825180:CDS
AGAAGCAGCAAAACAGATTC+TGG 0.389790 5_3:-64906779 Msa0825180:CDS
AAAAGCGAAGAAGTGATATA+AGG 0.389812 5_3:+64907066 None:intergenic
CTCTTATAGTATTGAACATC+AGG 0.390571 5_3:+64908570 None:intergenic
AGCATTACTAAGTGTGTGTT+TGG 0.411616 5_3:-64906612 Msa0825180:three_prime_UTR
CCGATTTCGAAGCAAGTGTT+GGG 0.411778 5_3:-64906943 Msa0825180:CDS
AGAAGAATAAGAAGATAAGA+TGG 0.418675 5_3:+64908785 None:intergenic
CTCGAAAAGACCTTGTTCTC+TGG 0.419190 5_3:-64907170 Msa0825180:CDS
TTAGCATTGTTGGACTGCTT+CGG 0.427662 5_3:+64907214 None:intergenic
CGATACACATAGGCAGGCTT+TGG 0.432959 5_3:-64907109 Msa0825180:CDS
GATGTGAATTGATTTATGTT+TGG 0.433229 5_3:-64906518 Msa0825180:three_prime_UTR
CCAACACTTGCTTCGAAATC+GGG 0.439844 5_3:+64906944 None:intergenic
GTGATATAAGGTTGAACCTC+TGG 0.443073 5_3:+64907078 None:intergenic
CCCGATTTCGAAGCAAGTGT+TGG 0.467573 5_3:-64906944 Msa0825180:CDS
ATCATTTGAACTGTTAAGAT+AGG 0.468826 5_3:+64907263 None:intergenic
TTCCTCTCCTACTTCTCTCT+CGG 0.469965 5_3:+64906894 None:intergenic
CAATACTATAAGAGCAAGAA+TGG 0.480003 5_3:-64908561 Msa0825180:CDS
TTACCTTAAAACTTTCTATG+AGG 0.484560 5_3:-64907142 Msa0825180:CDS
ATCAAGCATGAGATTCTTGT+GGG 0.484852 5_3:+64908650 None:intergenic
ACAGATTCTGGAAGCCATCA+AGG 0.490658 5_3:-64906767 Msa0825180:CDS
AAAGACATAAACATTTGTCT+TGG 0.497744 5_3:-64908858 Msa0825180:five_prime_UTR
AGAAACAGAAGAACGAGAAG+TGG 0.502938 5_3:+64908743 None:intergenic
AGATGGAAAGTGATGAATGT+TGG 0.508445 5_3:+64908802 None:intergenic
ATCTTGGTTCAAGCTTTCCA+AGG 0.509846 5_3:-64906813 Msa0825180:CDS
GTGCATGCTGAGCTACCAAC+AGG 0.516588 5_3:-64907315 Msa0825180:CDS
TTGTGGGGTGCTGAAACAAA+TGG 0.519655 5_3:+64908666 None:intergenic
TCCTACTTCTCTCTCGGCAC+AGG 0.520467 5_3:+64906900 None:intergenic
AAAGTGATGAATGTTGGAAA+TGG 0.528882 5_3:+64908808 None:intergenic
GCTTCTGGACCAAACAACCT+TGG 0.539958 5_3:+64906796 None:intergenic
ACAGGGGAATCAGTGTTGCT+TGG 0.543335 5_3:+64908510 None:intergenic
TAAACTCGATACACATAGGC+AGG 0.546034 5_3:-64907115 Msa0825180:CDS
ATTCAGACAGAAAATGATGA+AGG 0.546561 5_3:-64907003 Msa0825180:CDS
GGTAATCTGGCCAGAGAACA+AGG 0.552840 5_3:+64907160 None:intergenic
GCAAGTGTTGGGGATATGGT+TGG 0.554687 5_3:-64906932 Msa0825180:CDS
ATGCGAACAAATATAGCGGT+TGG 0.559048 5_3:+64907024 None:intergenic
TAGTAGAAACAAGACTATTG+AGG 0.564219 5_3:-64906839 Msa0825180:CDS
ACATCAGGTGGAAGTGAACA+AGG 0.566315 5_3:+64908585 None:intergenic
GCCTGTGCCGAGAGAGAAGT+AGG 0.571524 5_3:-64906901 Msa0825180:CDS
ATATCTAATGATCCTCCTGT+TGG 0.577783 5_3:+64907300 None:intergenic
GATCAAGCATGAGATTCTTG+TGG 0.577920 5_3:+64908649 None:intergenic
TAAGTGTGTGTTTGGTATGA+CGG 0.586600 5_3:-64906604 Msa0825180:three_prime_UTR
TGCCGAGAGAGAAGTAGGAG+AGG 0.601529 5_3:-64906896 Msa0825180:CDS
TTATAGTATTGAACATCAGG+TGG 0.606078 5_3:+64908573 None:intergenic
ATGGATACCCAGAAGAAGCA+AGG 0.631772 5_3:-64908711 Msa0825180:CDS
CGATTTCGAAGCAAGTGTTG+GGG 0.634468 5_3:-64906942 Msa0825180:CDS
ACACTAAACTCGATACACAT+AGG 0.635620 5_3:-64907119 Msa0825180:CDS
CATTTGTCTTGGTTACACAA+TGG 0.648976 5_3:-64908847 Msa0825180:exon
CATGCTGAGCTACCAACAGG+AGG 0.671949 5_3:-64907312 Msa0825180:CDS
CTGAATGCGAACAAATATAG+CGG 0.692980 5_3:+64907020 None:intergenic
CGGTTGGAGATGCAACGCTG+CGG 0.725888 5_3:+64907040 None:intergenic
TCAAGCATGAGATTCTTGTG+GGG 0.732179 5_3:+64908651 None:intergenic
GTGTGTGTTTGGTATGACGG+TGG 0.740191 5_3:-64906601 Msa0825180:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTATGTTATTTTATT+AGG - chr5_3:64907029-64907048 Msa0825180:CDS 10.0%
!!! TATTGTTATGTTATTTTATT+AGG - chr5_3:64907029-64907048 Msa0825180:CDS 10.0%
!! TTTAAAAGCAAAAAGTAGAA+AGG + chr5_3:64906955-64906974 None:intergenic 20.0%
!! TATACTCTAACATGAAAATA+TGG + chr5_3:64906981-64907000 None:intergenic 20.0%
!! TTCATGTTAGAGTATATATT+TGG - chr5_3:64906985-64907004 Msa0825180:CDS 20.0%
!! GAAGAAAAATAATGTATTTG+AGG - chr5_3:64907632-64907651 Msa0825180:intron 20.0%
!!! GATCTATTTGGTATAAATTT+TGG + chr5_3:64907917-64907936 None:intergenic 20.0%
!! TCAACAAAAATTGATCTATT+TGG + chr5_3:64907929-64907948 None:intergenic 20.0%
!! CTCAAAATATAAGCAAAAAT+GGG + chr5_3:64907952-64907971 None:intergenic 20.0%
!! TCTCAAAATATAAGCAAAAA+TGG + chr5_3:64907953-64907972 None:intergenic 20.0%
!!! ATTTTTGCTTATATTTTGAG+AGG - chr5_3:64907952-64907971 Msa0825180:intron 20.0%
!!! TTTTTGCTTATATTTTGAGA+GGG - chr5_3:64907953-64907972 Msa0825180:intron 20.0%
!!! GTTTGAGATACAAATTTATT+TGG - chr5_3:64908701-64908720 Msa0825180:CDS 20.0%
!! TTTAAAAGCAAAAAGTAGAA+AGG + chr5_3:64906955-64906974 None:intergenic 20.0%
!! TATACTCTAACATGAAAATA+TGG + chr5_3:64906981-64907000 None:intergenic 20.0%
!! TTCATGTTAGAGTATATATT+TGG - chr5_3:64906985-64907004 Msa0825180:CDS 20.0%
!! GAAGAAAAATAATGTATTTG+AGG - chr5_3:64907632-64907651 Msa0825180:intron 20.0%
!!! GATCTATTTGGTATAAATTT+TGG + chr5_3:64907917-64907936 None:intergenic 20.0%
!! TCAACAAAAATTGATCTATT+TGG + chr5_3:64907929-64907948 None:intergenic 20.0%
!! CTCAAAATATAAGCAAAAAT+GGG + chr5_3:64907952-64907971 None:intergenic 20.0%
!! TCTCAAAATATAAGCAAAAA+TGG + chr5_3:64907953-64907972 None:intergenic 20.0%
!!! ATTTTTGCTTATATTTTGAG+AGG - chr5_3:64907952-64907971 Msa0825180:intron 20.0%
!!! TTTTTGCTTATATTTTGAGA+GGG - chr5_3:64907953-64907972 Msa0825180:intron 20.0%
!!! GTTTGAGATACAAATTTATT+TGG - chr5_3:64908701-64908720 Msa0825180:CDS 20.0%
! AAAGACATAAACATTTGTCT+TGG - chr5_3:64906576-64906595 Msa0825180:three_prime_UTR 25.0%
! AGAAGAATAAGAAGATAAGA+TGG + chr5_3:64906652-64906671 None:intergenic 25.0%
!! TTCTGTTTCTTGTTCTATAA+TGG - chr5_3:64906704-64906723 Msa0825180:three_prime_UTR 25.0%
! AAAGTAGAAAGGAAATTACA+GGG + chr5_3:64906944-64906963 None:intergenic 25.0%
! AAAAGTAGAAAGGAAATTAC+AGG + chr5_3:64906945-64906964 None:intergenic 25.0%
! TATATATTTGGTTGAGAACT+AGG - chr5_3:64906997-64907016 Msa0825180:CDS 25.0%
! ATGCAAATAACCAAATTAAG+TGG + chr5_3:64907208-64907227 None:intergenic 25.0%
! ACAGCTATCTTTCTTTATAA+AGG - chr5_3:64907579-64907598 Msa0825180:intron 25.0%
! TTATGTCAGTATATACAAAC+TGG + chr5_3:64907658-64907677 None:intergenic 25.0%
!!! TGTCTGTTTTGTTATAGAAA+TGG - chr5_3:64907817-64907836 Msa0825180:intron 25.0%
! TTCAGCATAAAACAATAACA+AGG + chr5_3:64908039-64908058 None:intergenic 25.0%
!! ATCACAGATAGATTTTGTAA+TGG - chr5_3:64908088-64908107 Msa0825180:intron 25.0%
!! TCACAGATAGATTTTGTAAT+GGG - chr5_3:64908089-64908108 Msa0825180:intron 25.0%
! ATCATTTGAACTGTTAAGAT+AGG + chr5_3:64908174-64908193 None:intergenic 25.0%
!!! TAGAAAGTTTTAAGGTAATC+TGG + chr5_3:64908290-64908309 None:intergenic 25.0%
! TTACCTTAAAACTTTCTATG+AGG - chr5_3:64908292-64908311 Msa0825180:intron 25.0%
!! GATGTGAATTGATTTATGTT+TGG - chr5_3:64908916-64908935 Msa0825180:five_prime_UTR 25.0%
! AAAGACATAAACATTTGTCT+TGG - chr5_3:64906576-64906595 Msa0825180:three_prime_UTR 25.0%
! AGAAGAATAAGAAGATAAGA+TGG + chr5_3:64906652-64906671 None:intergenic 25.0%
!! TTCTGTTTCTTGTTCTATAA+TGG - chr5_3:64906704-64906723 Msa0825180:three_prime_UTR 25.0%
! AAAGTAGAAAGGAAATTACA+GGG + chr5_3:64906944-64906963 None:intergenic 25.0%
! AAAAGTAGAAAGGAAATTAC+AGG + chr5_3:64906945-64906964 None:intergenic 25.0%
! TATATATTTGGTTGAGAACT+AGG - chr5_3:64906997-64907016 Msa0825180:CDS 25.0%
! ATGCAAATAACCAAATTAAG+TGG + chr5_3:64907208-64907227 None:intergenic 25.0%
! ACAGCTATCTTTCTTTATAA+AGG - chr5_3:64907579-64907598 Msa0825180:intron 25.0%
! TTATGTCAGTATATACAAAC+TGG + chr5_3:64907658-64907677 None:intergenic 25.0%
!!! TGTCTGTTTTGTTATAGAAA+TGG - chr5_3:64907817-64907836 Msa0825180:intron 25.0%
! TTCAGCATAAAACAATAACA+AGG + chr5_3:64908039-64908058 None:intergenic 25.0%
!! ATCACAGATAGATTTTGTAA+TGG - chr5_3:64908088-64908107 Msa0825180:intron 25.0%
!! TCACAGATAGATTTTGTAAT+GGG - chr5_3:64908089-64908108 Msa0825180:intron 25.0%
! ATCATTTGAACTGTTAAGAT+AGG + chr5_3:64908174-64908193 None:intergenic 25.0%
!!! TAGAAAGTTTTAAGGTAATC+TGG + chr5_3:64908290-64908309 None:intergenic 25.0%
! TTACCTTAAAACTTTCTATG+AGG - chr5_3:64908292-64908311 Msa0825180:intron 25.0%
!! GATGTGAATTGATTTATGTT+TGG - chr5_3:64908916-64908935 Msa0825180:five_prime_UTR 25.0%
AAAGTGATGAATGTTGGAAA+TGG + chr5_3:64906629-64906648 None:intergenic 30.0%
CTTCTTATTCTTCTTCTTCA+AGG - chr5_3:64906658-64906677 Msa0825180:three_prime_UTR 30.0%
! TTATAGTATTGAACATCAGG+TGG + chr5_3:64906864-64906883 None:intergenic 30.0%
! CTCTTATAGTATTGAACATC+AGG + chr5_3:64906867-64906886 None:intergenic 30.0%
CAATACTATAAGAGCAAGAA+TGG - chr5_3:64906873-64906892 Msa0825180:CDS 30.0%
AAGTAGAAAGGAAATTACAG+GGG + chr5_3:64906943-64906962 None:intergenic 30.0%
GTGATTTCAACCACTTAATT+TGG - chr5_3:64907195-64907214 Msa0825180:CDS 30.0%
! TTGCTTACTTTAGAGAATGA+AGG - chr5_3:64907284-64907303 Msa0825180:CDS 30.0%
ACATTACCAATTTGATTCAG+TGG + chr5_3:64907311-64907330 None:intergenic 30.0%
!! TGATTCTTAAAGTATTGCAC+TGG - chr5_3:64907418-64907437 Msa0825180:intron 30.0%
ACATGTTAGACTGTTTGATA+GGG - chr5_3:64907679-64907698 Msa0825180:intron 30.0%
!! ATACATCAGCTTTTGTTTAC+CGG + chr5_3:64907889-64907908 None:intergenic 30.0%
AACAATAACAAGGTTCTGTT+TGG + chr5_3:64908029-64908048 None:intergenic 30.0%
AGAACAAGAATTAGCATTGT+TGG + chr5_3:64908233-64908252 None:intergenic 30.0%
! TGTCCTCATAGAAAGTTTTA+AGG + chr5_3:64908298-64908317 None:intergenic 30.0%
AAAAGCGAAGAAGTGATATA+AGG + chr5_3:64908371-64908390 None:intergenic 30.0%
ATTCAGACAGAAAATGATGA+AGG - chr5_3:64908431-64908450 Msa0825180:intron 30.0%
TAGTAGAAACAAGACTATTG+AGG - chr5_3:64908595-64908614 Msa0825180:CDS 30.0%
AACAGTAAAATACTCCTTGA+TGG + chr5_3:64908684-64908703 None:intergenic 30.0%
AAAGTGATGAATGTTGGAAA+TGG + chr5_3:64906629-64906648 None:intergenic 30.0%
CTTCTTATTCTTCTTCTTCA+AGG - chr5_3:64906658-64906677 Msa0825180:three_prime_UTR 30.0%
! TTATAGTATTGAACATCAGG+TGG + chr5_3:64906864-64906883 None:intergenic 30.0%
! CTCTTATAGTATTGAACATC+AGG + chr5_3:64906867-64906886 None:intergenic 30.0%
CAATACTATAAGAGCAAGAA+TGG - chr5_3:64906873-64906892 Msa0825180:CDS 30.0%
AAGTAGAAAGGAAATTACAG+GGG + chr5_3:64906943-64906962 None:intergenic 30.0%
GTGATTTCAACCACTTAATT+TGG - chr5_3:64907195-64907214 Msa0825180:CDS 30.0%
! TTGCTTACTTTAGAGAATGA+AGG - chr5_3:64907284-64907303 Msa0825180:CDS 30.0%
ACATTACCAATTTGATTCAG+TGG + chr5_3:64907311-64907330 None:intergenic 30.0%
!! TGATTCTTAAAGTATTGCAC+TGG - chr5_3:64907418-64907437 Msa0825180:intron 30.0%
ACATGTTAGACTGTTTGATA+GGG - chr5_3:64907679-64907698 Msa0825180:intron 30.0%
!! ATACATCAGCTTTTGTTTAC+CGG + chr5_3:64907889-64907908 None:intergenic 30.0%
AACAATAACAAGGTTCTGTT+TGG + chr5_3:64908029-64908048 None:intergenic 30.0%
AGAACAAGAATTAGCATTGT+TGG + chr5_3:64908233-64908252 None:intergenic 30.0%
! TGTCCTCATAGAAAGTTTTA+AGG + chr5_3:64908298-64908317 None:intergenic 30.0%
AAAAGCGAAGAAGTGATATA+AGG + chr5_3:64908371-64908390 None:intergenic 30.0%
ATTCAGACAGAAAATGATGA+AGG - chr5_3:64908431-64908450 Msa0825180:intron 30.0%
TAGTAGAAACAAGACTATTG+AGG - chr5_3:64908595-64908614 Msa0825180:CDS 30.0%
AACAGTAAAATACTCCTTGA+TGG + chr5_3:64908684-64908703 None:intergenic 30.0%
CATTTGTCTTGGTTACACAA+TGG - chr5_3:64906587-64906606 Msa0825180:three_prime_UTR 35.0%
AGATGGAAAGTGATGAATGT+TGG + chr5_3:64906635-64906654 None:intergenic 35.0%
ATCAAGCATGAGATTCTTGT+GGG + chr5_3:64906787-64906806 None:intergenic 35.0%
! TAGAGTGTCTAAAGTGAGTT+TGG + chr5_3:64907111-64907130 None:intergenic 35.0%
TTACTAACTACTCACCTTAG+AGG + chr5_3:64907155-64907174 None:intergenic 35.0%
!! TCTTAAAGTATTGCACTGGT+TGG - chr5_3:64907422-64907441 Msa0825180:intron 35.0%
TTCCCTAGTTGTGATTGTTT+GGG - chr5_3:64907457-64907476 Msa0825180:intron 35.0%
TACCCAAACAATCACAACTA+GGG + chr5_3:64907462-64907481 None:intergenic 35.0%
ATACCCAAACAATCACAACT+AGG + chr5_3:64907463-64907482 None:intergenic 35.0%
CACATGTTAGACTGTTTGAT+AGG - chr5_3:64907678-64907697 Msa0825180:intron 35.0%
ACAACTTATAGACATGTCCT+AGG + chr5_3:64907717-64907736 None:intergenic 35.0%
!!! CTTTGCTTATATTTTGGGAC+AGG + chr5_3:64907865-64907884 None:intergenic 35.0%
! CCGGTCTTTGCTTATATTTT+GGG + chr5_3:64907870-64907889 None:intergenic 35.0%
CCCAAAATATAAGCAAAGAC+CGG - chr5_3:64907867-64907886 Msa0825180:intron 35.0%
! ACCGGTCTTTGCTTATATTT+TGG + chr5_3:64907871-64907890 None:intergenic 35.0%
!! TAGATTTTGTAATGGGAAGC+TGG - chr5_3:64908096-64908115 Msa0825180:intron 35.0%
!! AGATTTTGTAATGGGAAGCT+GGG - chr5_3:64908097-64908116 Msa0825180:intron 35.0%
ATATCTAATGATCCTCCTGT+TGG + chr5_3:64908137-64908156 None:intergenic 35.0%
ACACTAAACTCGATACACAT+AGG - chr5_3:64908315-64908334 Msa0825180:intron 35.0%
CTGAATGCGAACAAATATAG+CGG + chr5_3:64908417-64908436 None:intergenic 35.0%
! AAGACTATTGAGGTTGATCT+TGG - chr5_3:64908605-64908624 Msa0825180:CDS 35.0%
AGCATTACTAAGTGTGTGTT+TGG - chr5_3:64908822-64908841 Msa0825180:CDS 35.0%
TAAGTGTGTGTTTGGTATGA+CGG - chr5_3:64908830-64908849 Msa0825180:CDS 35.0%
CATTTGTCTTGGTTACACAA+TGG - chr5_3:64906587-64906606 Msa0825180:three_prime_UTR 35.0%
AGATGGAAAGTGATGAATGT+TGG + chr5_3:64906635-64906654 None:intergenic 35.0%
ATCAAGCATGAGATTCTTGT+GGG + chr5_3:64906787-64906806 None:intergenic 35.0%
! TAGAGTGTCTAAAGTGAGTT+TGG + chr5_3:64907111-64907130 None:intergenic 35.0%
TTACTAACTACTCACCTTAG+AGG + chr5_3:64907155-64907174 None:intergenic 35.0%
!! TCTTAAAGTATTGCACTGGT+TGG - chr5_3:64907422-64907441 Msa0825180:intron 35.0%
TTCCCTAGTTGTGATTGTTT+GGG - chr5_3:64907457-64907476 Msa0825180:intron 35.0%
TACCCAAACAATCACAACTA+GGG + chr5_3:64907462-64907481 None:intergenic 35.0%
ATACCCAAACAATCACAACT+AGG + chr5_3:64907463-64907482 None:intergenic 35.0%
CACATGTTAGACTGTTTGAT+AGG - chr5_3:64907678-64907697 Msa0825180:intron 35.0%
ACAACTTATAGACATGTCCT+AGG + chr5_3:64907717-64907736 None:intergenic 35.0%
!!! CTTTGCTTATATTTTGGGAC+AGG + chr5_3:64907865-64907884 None:intergenic 35.0%
! CCGGTCTTTGCTTATATTTT+GGG + chr5_3:64907870-64907889 None:intergenic 35.0%
CCCAAAATATAAGCAAAGAC+CGG - chr5_3:64907867-64907886 Msa0825180:intron 35.0%
! ACCGGTCTTTGCTTATATTT+TGG + chr5_3:64907871-64907890 None:intergenic 35.0%
!! TAGATTTTGTAATGGGAAGC+TGG - chr5_3:64908096-64908115 Msa0825180:intron 35.0%
!! AGATTTTGTAATGGGAAGCT+GGG - chr5_3:64908097-64908116 Msa0825180:intron 35.0%
ATATCTAATGATCCTCCTGT+TGG + chr5_3:64908137-64908156 None:intergenic 35.0%
ACACTAAACTCGATACACAT+AGG - chr5_3:64908315-64908334 Msa0825180:intron 35.0%
CTGAATGCGAACAAATATAG+CGG + chr5_3:64908417-64908436 None:intergenic 35.0%
! AAGACTATTGAGGTTGATCT+TGG - chr5_3:64908605-64908624 Msa0825180:CDS 35.0%
AGCATTACTAAGTGTGTGTT+TGG - chr5_3:64908822-64908841 Msa0825180:CDS 35.0%
TAAGTGTGTGTTTGGTATGA+CGG - chr5_3:64908830-64908849 Msa0825180:CDS 35.0%
AGAAACAGAAGAACGAGAAG+TGG + chr5_3:64906694-64906713 None:intergenic 40.0%
! GCAAGGTCGTAGCAAATTTT+TGG - chr5_3:64906740-64906759 Msa0825180:exon 40.0%
TCAAGCATGAGATTCTTGTG+GGG + chr5_3:64906786-64906805 None:intergenic 40.0%
GATCAAGCATGAGATTCTTG+TGG + chr5_3:64906788-64906807 None:intergenic 40.0%
TCAACCATGTTTCTCCTCTA+AGG - chr5_3:64907138-64907157 Msa0825180:CDS 40.0%
GAAGGACCACTGAATCAAAT+TGG - chr5_3:64907302-64907321 Msa0825180:CDS 40.0%
GTTCCCTAGTTGTGATTGTT+TGG - chr5_3:64907456-64907475 Msa0825180:intron 40.0%
CTTATAGACATGTCCTAGGT+TGG + chr5_3:64907713-64907732 None:intergenic 40.0%
CTTATTTCCGTAAGTTCTCG+AGG - chr5_3:64907743-64907762 Msa0825180:intron 40.0%
AAGCTATCCTCGAGAACTTA+CGG + chr5_3:64907753-64907772 None:intergenic 40.0%
ATCAGCTCGAACACGAAATT+TGG + chr5_3:64908200-64908219 None:intergenic 40.0%
TTAGCATTGTTGGACTGCTT+CGG + chr5_3:64908223-64908242 None:intergenic 40.0%
TAAACTCGATACACATAGGC+AGG - chr5_3:64908319-64908338 Msa0825180:intron 40.0%
!! GGCTTTGGAAAAAATTCCAG+AGG - chr5_3:64908340-64908359 Msa0825180:intron 40.0%
GTGATATAAGGTTGAACCTC+TGG + chr5_3:64908359-64908378 None:intergenic 40.0%
ATGCGAACAAATATAGCGGT+TGG + chr5_3:64908413-64908432 None:intergenic 40.0%
ATCTTGGTTCAAGCTTTCCA+AGG - chr5_3:64908621-64908640 Msa0825180:CDS 40.0%
TCAAGCTTTCCAAGGTTGTT+TGG - chr5_3:64908629-64908648 Msa0825180:CDS 40.0%
!! AGAATCTGTTTTGCTGCTTC+TGG + chr5_3:64908656-64908675 None:intergenic 40.0%
AGAAGCAGCAAAACAGATTC+TGG - chr5_3:64908655-64908674 Msa0825180:CDS 40.0%
AGAAACAGAAGAACGAGAAG+TGG + chr5_3:64906694-64906713 None:intergenic 40.0%
! GCAAGGTCGTAGCAAATTTT+TGG - chr5_3:64906740-64906759 Msa0825180:exon 40.0%
TCAAGCATGAGATTCTTGTG+GGG + chr5_3:64906786-64906805 None:intergenic 40.0%
GATCAAGCATGAGATTCTTG+TGG + chr5_3:64906788-64906807 None:intergenic 40.0%
TCAACCATGTTTCTCCTCTA+AGG - chr5_3:64907138-64907157 Msa0825180:CDS 40.0%
GAAGGACCACTGAATCAAAT+TGG - chr5_3:64907302-64907321 Msa0825180:CDS 40.0%
GTTCCCTAGTTGTGATTGTT+TGG - chr5_3:64907456-64907475 Msa0825180:intron 40.0%
CTTATAGACATGTCCTAGGT+TGG + chr5_3:64907713-64907732 None:intergenic 40.0%
CTTATTTCCGTAAGTTCTCG+AGG - chr5_3:64907743-64907762 Msa0825180:intron 40.0%
AAGCTATCCTCGAGAACTTA+CGG + chr5_3:64907753-64907772 None:intergenic 40.0%
ATCAGCTCGAACACGAAATT+TGG + chr5_3:64908200-64908219 None:intergenic 40.0%
TTAGCATTGTTGGACTGCTT+CGG + chr5_3:64908223-64908242 None:intergenic 40.0%
TAAACTCGATACACATAGGC+AGG - chr5_3:64908319-64908338 Msa0825180:intron 40.0%
!! GGCTTTGGAAAAAATTCCAG+AGG - chr5_3:64908340-64908359 Msa0825180:intron 40.0%
GTGATATAAGGTTGAACCTC+TGG + chr5_3:64908359-64908378 None:intergenic 40.0%
ATGCGAACAAATATAGCGGT+TGG + chr5_3:64908413-64908432 None:intergenic 40.0%
ATCTTGGTTCAAGCTTTCCA+AGG - chr5_3:64908621-64908640 Msa0825180:CDS 40.0%
TCAAGCTTTCCAAGGTTGTT+TGG - chr5_3:64908629-64908648 Msa0825180:CDS 40.0%
!! AGAATCTGTTTTGCTGCTTC+TGG + chr5_3:64908656-64908675 None:intergenic 40.0%
AGAAGCAGCAAAACAGATTC+TGG - chr5_3:64908655-64908674 Msa0825180:CDS 40.0%
ATGGATACCCAGAAGAAGCA+AGG - chr5_3:64906723-64906742 Msa0825180:three_prime_UTR 45.0%
!! TTGTGGGGTGCTGAAACAAA+TGG + chr5_3:64906771-64906790 None:intergenic 45.0%
ACATCAGGTGGAAGTGAACA+AGG + chr5_3:64906852-64906871 None:intergenic 45.0%
CTCACCTTAGAGGAGAAACA+TGG + chr5_3:64907145-64907164 None:intergenic 45.0%
!! CTGTAGCCTAAGCCTTGTTT+AGG - chr5_3:64907386-64907405 Msa0825180:intron 45.0%
TCACAAGACGCTCCTAAACA+AGG + chr5_3:64907401-64907420 None:intergenic 45.0%
TAGGGCTTATGAACCAACCT+AGG - chr5_3:64907697-64907716 Msa0825180:intron 45.0%
!! AGAACAAGGTCTTTTCGAGG+TGG + chr5_3:64908263-64908282 None:intergenic 45.0%
! CAGAGAACAAGGTCTTTTCG+AGG + chr5_3:64908266-64908285 None:intergenic 45.0%
CTCGAAAAGACCTTGTTCTC+TGG - chr5_3:64908264-64908283 Msa0825180:intron 45.0%
GCTTCGAAATCGGGTCAATA+TGG + chr5_3:64908484-64908503 None:intergenic 45.0%
CCAACACTTGCTTCGAAATC+GGG + chr5_3:64908493-64908512 None:intergenic 45.0%
CCCAACACTTGCTTCGAAAT+CGG + chr5_3:64908494-64908513 None:intergenic 45.0%
CCGATTTCGAAGCAAGTGTT+GGG - chr5_3:64908491-64908510 Msa0825180:intron 45.0%
! CGATTTCGAAGCAAGTGTTG+GGG - chr5_3:64908492-64908511 Msa0825180:intron 45.0%
TTCCTCTCCTACTTCTCTCT+CGG + chr5_3:64908543-64908562 None:intergenic 45.0%
! ACAGATTCTGGAAGCCATCA+AGG - chr5_3:64908667-64908686 Msa0825180:CDS 45.0%
ATGGATACCCAGAAGAAGCA+AGG - chr5_3:64906723-64906742 Msa0825180:three_prime_UTR 45.0%
!! TTGTGGGGTGCTGAAACAAA+TGG + chr5_3:64906771-64906790 None:intergenic 45.0%
ACATCAGGTGGAAGTGAACA+AGG + chr5_3:64906852-64906871 None:intergenic 45.0%
CTCACCTTAGAGGAGAAACA+TGG + chr5_3:64907145-64907164 None:intergenic 45.0%
!! CTGTAGCCTAAGCCTTGTTT+AGG - chr5_3:64907386-64907405 Msa0825180:intron 45.0%
TCACAAGACGCTCCTAAACA+AGG + chr5_3:64907401-64907420 None:intergenic 45.0%
TAGGGCTTATGAACCAACCT+AGG - chr5_3:64907697-64907716 Msa0825180:intron 45.0%
!! AGAACAAGGTCTTTTCGAGG+TGG + chr5_3:64908263-64908282 None:intergenic 45.0%
! CAGAGAACAAGGTCTTTTCG+AGG + chr5_3:64908266-64908285 None:intergenic 45.0%
CTCGAAAAGACCTTGTTCTC+TGG - chr5_3:64908264-64908283 Msa0825180:intron 45.0%
GCTTCGAAATCGGGTCAATA+TGG + chr5_3:64908484-64908503 None:intergenic 45.0%
CCAACACTTGCTTCGAAATC+GGG + chr5_3:64908493-64908512 None:intergenic 45.0%
CCCAACACTTGCTTCGAAAT+CGG + chr5_3:64908494-64908513 None:intergenic 45.0%
CCGATTTCGAAGCAAGTGTT+GGG - chr5_3:64908491-64908510 Msa0825180:intron 45.0%
! CGATTTCGAAGCAAGTGTTG+GGG - chr5_3:64908492-64908511 Msa0825180:intron 45.0%
TTCCTCTCCTACTTCTCTCT+CGG + chr5_3:64908543-64908562 None:intergenic 45.0%
! ACAGATTCTGGAAGCCATCA+AGG - chr5_3:64908667-64908686 Msa0825180:CDS 45.0%
GCTACGACCTTGCTTCTTCT+GGG + chr5_3:64906733-64906752 None:intergenic 50.0%
TGCTACGACCTTGCTTCTTC+TGG + chr5_3:64906734-64906753 None:intergenic 50.0%
! ACAGGGGAATCAGTGTTGCT+TGG + chr5_3:64906927-64906946 None:intergenic 50.0%
GACGCTCCTAAACAAGGCTT+AGG + chr5_3:64907395-64907414 None:intergenic 50.0%
GGTAATCTGGCCAGAGAACA+AGG + chr5_3:64908277-64908296 None:intergenic 50.0%
CGATACACATAGGCAGGCTT+TGG - chr5_3:64908325-64908344 Msa0825180:intron 50.0%
CCCGATTTCGAAGCAAGTGT+TGG - chr5_3:64908490-64908509 Msa0825180:intron 50.0%
!! CGAAGCAAGTGTTGGGGATA+TGG - chr5_3:64908498-64908517 Msa0825180:intron 50.0%
!! GCAAGTGTTGGGGATATGGT+TGG - chr5_3:64908502-64908521 Msa0825180:intron 50.0%
GCTTCTGGACCAAACAACCT+TGG + chr5_3:64908641-64908660 None:intergenic 50.0%
GTGTGTGTTTGGTATGACGG+TGG - chr5_3:64908833-64908852 Msa0825180:exon 50.0%
GCTACGACCTTGCTTCTTCT+GGG + chr5_3:64906733-64906752 None:intergenic 50.0%
TGCTACGACCTTGCTTCTTC+TGG + chr5_3:64906734-64906753 None:intergenic 50.0%
! ACAGGGGAATCAGTGTTGCT+TGG + chr5_3:64906927-64906946 None:intergenic 50.0%
GACGCTCCTAAACAAGGCTT+AGG + chr5_3:64907395-64907414 None:intergenic 50.0%
GGTAATCTGGCCAGAGAACA+AGG + chr5_3:64908277-64908296 None:intergenic 50.0%
CGATACACATAGGCAGGCTT+TGG - chr5_3:64908325-64908344 Msa0825180:intron 50.0%
CCCGATTTCGAAGCAAGTGT+TGG - chr5_3:64908490-64908509 Msa0825180:intron 50.0%
!! CGAAGCAAGTGTTGGGGATA+TGG - chr5_3:64908498-64908517 Msa0825180:intron 50.0%
!! GCAAGTGTTGGGGATATGGT+TGG - chr5_3:64908502-64908521 Msa0825180:intron 50.0%
GCTTCTGGACCAAACAACCT+TGG + chr5_3:64908641-64908660 None:intergenic 50.0%
GTGTGTGTTTGGTATGACGG+TGG - chr5_3:64908833-64908852 Msa0825180:exon 50.0%
GTGCATGCTGAGCTACCAAC+AGG - chr5_3:64908119-64908138 Msa0825180:intron 55.0%
CATGCTGAGCTACCAACAGG+AGG - chr5_3:64908122-64908141 Msa0825180:intron 55.0%
TCCTACTTCTCTCTCGGCAC+AGG + chr5_3:64908537-64908556 None:intergenic 55.0%
TGCCGAGAGAGAAGTAGGAG+AGG - chr5_3:64908538-64908557 Msa0825180:CDS 55.0%
GTGCATGCTGAGCTACCAAC+AGG - chr5_3:64908119-64908138 Msa0825180:intron 55.0%
CATGCTGAGCTACCAACAGG+AGG - chr5_3:64908122-64908141 Msa0825180:intron 55.0%
TCCTACTTCTCTCTCGGCAC+AGG + chr5_3:64908537-64908556 None:intergenic 55.0%
TGCCGAGAGAGAAGTAGGAG+AGG - chr5_3:64908538-64908557 Msa0825180:CDS 55.0%
CGGTTGGAGATGCAACGCTG+CGG + chr5_3:64908397-64908416 None:intergenic 60.0%
GCCTGTGCCGAGAGAGAAGT+AGG - chr5_3:64908533-64908552 Msa0825180:CDS 60.0%
CGGTTGGAGATGCAACGCTG+CGG + chr5_3:64908397-64908416 None:intergenic 60.0%
GCCTGTGCCGAGAGAGAAGT+AGG - chr5_3:64908533-64908552 Msa0825180:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 64906515 64908941 64906515 ID=Msa0825180;Name=Msa0825180
chr5_3 mRNA 64906515 64908941 64906515 ID=Msa0825180-mRNA-1;Parent=Msa0825180;Name=Msa0825180-mRNA-1;_AED=0.01;_eAED=0.01;_QI=91|1|1|1|1|1|2|235|316
chr5_3 exon 64906515 64907361 64906515 ID=Msa0825180-mRNA-1:exon:20303;Parent=Msa0825180-mRNA-1
chr5_3 exon 64908512 64908941 64908512 ID=Msa0825180-mRNA-1:exon:20302;Parent=Msa0825180-mRNA-1
chr5_3 five_prime_UTR 64908851 64908941 64908851 ID=Msa0825180-mRNA-1:five_prime_utr;Parent=Msa0825180-mRNA-1
chr5_3 CDS 64908512 64908850 64908512 ID=Msa0825180-mRNA-1:cds;Parent=Msa0825180-mRNA-1
chr5_3 CDS 64906750 64907361 64906750 ID=Msa0825180-mRNA-1:cds;Parent=Msa0825180-mRNA-1
chr5_3 three_prime_UTR 64906515 64906749 64906515 ID=Msa0825180-mRNA-1:three_prime_utr;Parent=Msa0825180-mRNA-1
Gene Sequence

>Msa0825180

ATGGTAGTGATGATATGTAGCCATTTCCAACATTCATCACTTTCCATCTTATCTTCTTATTCTTCTTCTTCAAGGTTTCATCTTTCCACTTCTCGTTCTTCTGTTTCTTGTTCTATAATGGATACCCAGAAGAAGCAAGGTCGTAGCAAATTTTTGGAATTTCCATTTGTTTCAGCACCCCACAAGAATCTCATGCTTGATCTTGTTTCAACAGTTGAAGATCGCTTTGAATCTCATCTTCTTCCTTGTTCACTTCCACCTGATGTTCAATACTATAAGAGCAAGAATGGTACTTCTCAAGTTTCATTGCATATTAGACCAAGCAACACTGATTCCCCTATAGATTTTGTAATGGGAAGCTGGGTGCATGCTGAGCTACCAACAGGAGGATCATTAGATATAACATCTCTTTCAGCCTATCTTAACAGTTCAAATGATGCACCAAATTTCGTGTTCGAGCTGATCCGAAGCAGTCCAACAATGCTAATTCTTGTTCTTGATTTGCCACCTCGAAAAGACCTTGTTCTCTGGCCAGATTACCTTAAAACTTTCTATGAGGACACTAAACTCGATACACATAGGCAGGCTTTGGAAAAAATTCCAGAGGTTCAACCTTATATCACTTCTTCGCTTTTCATCCGCAGCGTTGCATCTCCAACCGCTATATTTGTTCGCATTCAGACAGAAAATGATGAAGGAGAGCGAATAGACGAGATCATCAAGAACCATATTGACCCGATTTCGAAGCAAGTGTTGGGGATATGGTTGGATCATTGTGCCTGTGCCGAGAGAGAAGTAGGAGAGGAAGATAAAGCTTATCTGATAAAAAGAGACAGTGTTAGTAGAAACAAGACTATTGAGGTTGATCTTGGTTCAAGCTTTCCAAGGTTGTTTGGTCCAGAAGCAGCAAAACAGATTCTGGAAGCCATCAAGGAGTATTTTACTGTTTGA
Protein sequence

>Msa0825180

MVVMICSHFQHSSLSILSSYSSSSRFHLSTSRSSVSCSIMDTQKKQGRSKFLEFPFVSAPHKNLMLDLVSTVEDRFESHLLPCSLPPDVQYYKSKNGTSQVSLHIRPSNTDSPIDFVMGSWVHAELPTGGSLDITSLSAYLNSSNDAPNFVFELIRSSPTMLILVLDLPPRKDLVLWPDYLKTFYEDTKLDTHRQALEKIPEVQPYITSSLFIRSVASPTAIFVRIQTENDEGERIDEIIKNHIDPISKQVLGIWLDHCACAEREVGEEDKAYLIKRDSVSRNKTIEVDLGSSFPRLFGPEAAKQILEAIKEYFTV*