AlfalfaGEDB | Msa0840750

M. sativa cultivar zhongmu-4 / Msa0840750

KEGG: K19323
GO: -
PFAM: PF09759.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840750	PNX60450.1	57.426	101	18	1	2	77	38	138	7.98e-27	106

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840750	A0A2K3K2H3	57.426	101	18	1	2	77	38	138	3.81e-27	106

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0657970	Msa0840750	0.818006	2.455359e-52	-8.615850e-47
Msa0789660	Msa0840750	0.812597	3.913921e-51	-8.615850e-47
Msa0824680	Msa0840750	0.800390	1.474173e-48	-8.615850e-47
Msa0840740	Msa0840750	0.807075	6.035121e-50	-8.615850e-47
Msa0840750	Msa1082500	0.804706	1.899203e-49	-8.615850e-47
Msa0840750	Msa1305870	0.827547	1.473198e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840750	MtrunA17_Chr7g0246491	58.416	101	17	1	2	77	389	489	4.85e-28	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840750	AT4G00231.1	42.424	99	32	1	2	75	375	473	6.28e-19	80.5

Find 18 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGACATAGTTGCATTTAT+TGG	0.222909	5_4:+16583156	Msa0840750:CDS
GTCATGGAAACAAAAGGTTT+TGG	0.287707	5_4:+16583550	Msa0840750:CDS
TTGGCAGCTGTGTTTATAGA+AGG	0.374605	5_4:+16583175	Msa0840750:CDS
TCTAGTCTAGTAAAAGATAA+AGG	0.380161	5_4:+16583491	Msa0840750:CDS
ATTTACAAGCATTGCGTGCC+TGG	0.405672	5_4:-16583390	None:intergenic
AGCATTGCGTGCCTGGTTCT+CGG	0.406257	5_4:-16583383	None:intergenic
AGACCAAGTACAGAAATTTC+AGG	0.407898	5_4:-16583344	None:intergenic
GTGCCTGAAATTTCTGTACT+TGG	0.416850	5_4:+16583341	Msa0840750:CDS
AGCACGCACAAGATGAAATC+AGG	0.458831	5_4:+16583199	Msa0840750:CDS
GATGAAATCAGGAGGAGGAA+TGG	0.483073	5_4:+16583210	Msa0840750:CDS
CGGAAGTTGATCCGAGAACC+AGG	0.501779	5_4:+16583372	Msa0840750:CDS
TGATTGTCTCGACAGAATCA+AGG	0.502244	5_4:+16583528	Msa0840750:CDS
ATCAAGGTCATGGAAACAAA+AGG	0.517751	5_4:+16583544	Msa0840750:CDS
TGTTGCTGAGTTAAAGCTGC+AGG	0.521628	5_4:+16583307	Msa0840750:CDS
TCTCGACAGAATCAAGGTCA+TGG	0.547258	5_4:+16583534	Msa0840750:CDS
CACAAGATGAAATCAGGAGG+AGG	0.598541	5_4:+16583205	Msa0840750:CDS
TTCTGTACTTGGTCTTCGAG+CGG	0.631603	5_4:+16583352	Msa0840750:CDS
ACGCACAAGATGAAATCAGG+AGG	0.692619	5_4:+16583202	Msa0840750:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTTATAGGATTATTTCGT+GGG	-	chr5_4:16583418-16583437	None:intergenic	25.0%
!	ATACATTACAACCAAGTTAT+AGG	-	chr5_4:16583431-16583450	None:intergenic	25.0%
!	TCTAGTCTAGTAAAAGATAA+AGG	+	chr5_4:16583491-16583510	Msa0840750:CDS	25.0%
	AGAGACATAGTTGCATTTAT+TGG	+	chr5_4:16583156-16583175	Msa0840750:CDS	30.0%
	CAAGTTATAGGATTATTTCG+TGG	-	chr5_4:16583419-16583438	None:intergenic	30.0%
	CACGAAATAATCCTATAACT+TGG	+	chr5_4:16583417-16583436	Msa0840750:intron	30.0%
!	TCATGGAAACAAAAGGTTTT+GGG	+	chr5_4:16583551-16583570	Msa0840750:CDS	30.0%
	AGACCAAGTACAGAAATTTC+AGG	-	chr5_4:16583347-16583366	None:intergenic	35.0%
	ATCAAGGTCATGGAAACAAA+AGG	+	chr5_4:16583544-16583563	Msa0840750:CDS	35.0%
!	GTCATGGAAACAAAAGGTTT+TGG	+	chr5_4:16583550-16583569	Msa0840750:CDS	35.0%
!	CATGGAAACAAAAGGTTTTG+GGG	+	chr5_4:16583552-16583571	Msa0840750:CDS	35.0%
	TTGGCAGCTGTGTTTATAGA+AGG	+	chr5_4:16583175-16583194	Msa0840750:CDS	40.0%
	GTGCCTGAAATTTCTGTACT+TGG	+	chr5_4:16583341-16583360	Msa0840750:CDS	40.0%
	CTAGACTAGAGACATTGTAC+TGG	-	chr5_4:16583481-16583500	None:intergenic	40.0%
	TGATTGTCTCGACAGAATCA+AGG	+	chr5_4:16583528-16583547	Msa0840750:CDS	40.0%
	AGCACGCACAAGATGAAATC+AGG	+	chr5_4:16583199-16583218	Msa0840750:CDS	45.0%
	ACGCACAAGATGAAATCAGG+AGG	+	chr5_4:16583202-16583221	Msa0840750:CDS	45.0%
	CACAAGATGAAATCAGGAGG+AGG	+	chr5_4:16583205-16583224	Msa0840750:CDS	45.0%
	GATGAAATCAGGAGGAGGAA+TGG	+	chr5_4:16583210-16583229	Msa0840750:CDS	45.0%
	TGTTGCTGAGTTAAAGCTGC+AGG	+	chr5_4:16583307-16583326	Msa0840750:CDS	45.0%
!	TTCTGTACTTGGTCTTCGAG+CGG	+	chr5_4:16583352-16583371	Msa0840750:CDS	45.0%
!	ATTTACAAGCATTGCGTGCC+TGG	-	chr5_4:16583393-16583412	None:intergenic	45.0%
	TCTCGACAGAATCAAGGTCA+TGG	+	chr5_4:16583534-16583553	Msa0840750:CDS	45.0%
!	CGGAAGTTGATCCGAGAACC+AGG	+	chr5_4:16583372-16583391	Msa0840750:CDS	55.0%
!	AGCATTGCGTGCCTGGTTCT+CGG	-	chr5_4:16583386-16583405	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	16583153	16583580	16583153	ID=Msa0840750;Name=Msa0840750
chr5_4	mRNA	16583153	16583580	16583153	ID=Msa0840750-mRNA-1;Parent=Msa0840750;Name=Msa0840750-mRNA-1;_AED=0.55;_eAED=0.55;_QI=0\|0\|0\|0.66\|0\|0\|3\|0\|106
chr5_4	exon	16583153	16583253	16583153	ID=Msa0840750-mRNA-1:exon:7136;Parent=Msa0840750-mRNA-1
chr5_4	exon	16583289	16583406	16583289	ID=Msa0840750-mRNA-1:exon:7137;Parent=Msa0840750-mRNA-1
chr5_4	exon	16583482	16583580	16583482	ID=Msa0840750-mRNA-1:exon:7138;Parent=Msa0840750-mRNA-1
chr5_4	CDS	16583153	16583253	16583153	ID=Msa0840750-mRNA-1:cds;Parent=Msa0840750-mRNA-1
chr5_4	CDS	16583289	16583406	16583289	ID=Msa0840750-mRNA-1:cds;Parent=Msa0840750-mRNA-1
chr5_4	CDS	16583482	16583580	16583482	ID=Msa0840750-mRNA-1:cds;Parent=Msa0840750-mRNA-1

Gene Sequence	>Msa0840750 ATGAGAGACATAGTTGCATTTATTGGCAGCTGTGTTTATAGAAGGAAGCACGCACAAGATGAAATCAGGAGGAGGAATGGAATTCTACTGCTATTGCAGCAAAAAGAGAATCAAAAGCTTGTTGCTGAGTTAAAGCTGCAGGAATCTGCTGATGTGCCTGAAATTTCTGTACTTGGTCTTCGAGCGGAAGTTGATCCGAGAACCAGGCACGCAATGCTTTACAATGTCTCTAGTCTAGTAAAAGATAAAGGAAATTATAATGATTTGATTGTCTCGACAGAATCAAGGTCATGGAAACAAAAGGTTTTGGGGATTGAG
Protein sequence	>Msa0840750 MRDIVAFIGSCVYRRKHAQDEIRRRNGILLLLQQKENQKLVAELKLQESADVPEISVLGLRAEVDPRTRHAMLYNVSSLVKDKGNYNDLIVSTESRSWKQKVLGIE