AlfalfaGEDB | Msa0847250

M. sativa cultivar zhongmu-4 / Msa0847250

KEGG: K16075
GO: GO:0003674 GO:0005215 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847250	XP_003614161.1	99.506	405	2	0	1	405	1	405	0.0	828

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847250	sp\|Q9ZPR4\|MRS25_ARATH	64.394	396	131	4	17	404	27	420	6.14e-164	469

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847250	G7JZ40	99.506	405	2	0	1	405	1	405	0.0	828

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0475490	Msa0847250	0.812063	5.119775e-51	-8.615850e-47
Msa0553460	Msa0847250	0.813993	1.932013e-51	-8.615850e-47
Msa0607540	Msa0847250	0.832568	8.775878e-56	-8.615850e-47
Msa0647830	Msa0847250	0.805843	1.097702e-49	-8.615850e-47
Msa0715720	Msa0847250	0.806386	8.437760e-50	-8.615850e-47
Msa0847250	Msa0886330	-0.817306	3.531681e-52	-8.615850e-47
Msa0847250	Msa1205130	0.812163	4.868430e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847250	MtrunA17_Chr5g0419611	99.506	405	2	0	1	405	1	405	0.0	828
Msa0847250	MtrunA17_Chr2g0333971	52.706	425	153	5	20	404	31	447	2.25e-142	413
Msa0847250	MtrunA17_Chr5g0393831	39.723	433	208	8	20	403	15	443	5.18e-95	291
Msa0847250	MtrunA17_Chr8g0380621	39.651	401	183	9	32	403	50	420	7.04e-83	259
Msa0847250	MtrunA17_Chr1g0187901	40.806	397	187	6	31	403	16	388	6.98e-75	238
Msa0847250	MtrunA17_Chr8g0384941	36.952	433	198	10	22	405	18	424	1.02e-74	239
Msa0847250	MtrunA17_Chr1g0206981	34.579	428	227	8	22	403	3	423	2.41e-73	235
Msa0847250	MtrunA17_Chr1g0165841	38.120	383	182	6	40	403	19	365	8.82e-67	216
Msa0847250	MtrunA17_Chr1g0165861	38.272	243	118	6	163	403	1	213	1.89e-43	150
Msa0847250	MtrunA17_Chr7g0260731	43.421	228	105	3	136	363	45	248	1.22e-40	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847250	AT2G03620.1	64.394	396	131	4	17	404	27	420	6.24e-165	469
Msa0847250	AT2G03620.2	64.394	396	131	4	17	404	27	420	6.24e-165	469
Msa0847250	AT1G16010.3	55.309	405	150	4	28	404	40	441	3.23e-147	425
Msa0847250	AT1G16010.1	55.309	405	150	4	28	404	40	441	3.23e-147	425
Msa0847250	AT1G16010.2	55.309	405	150	4	28	404	40	441	3.23e-147	425
Msa0847250	AT1G80900.2	52.482	423	151	4	20	404	32	442	4.89e-147	425
Msa0847250	AT1G80900.1	52.482	423	151	4	20	404	32	442	4.89e-147	425
Msa0847250	AT5G09690.1	40.909	396	191	4	26	403	14	384	4.79e-90	277
Msa0847250	AT5G09690.2	40.909	396	191	4	26	403	25	395	4.88e-90	278
Msa0847250	AT3G19640.1	40.868	438	185	11	40	403	46	483	7.54e-89	277

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Find 119 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGACTCATGTAGAC+TGG	0.504756	5_4:-28831345	None:intergenic
AAAGGTAAATGAACTGGAGA+TGG	0.505002	5_4:+28832722	Msa0847250:intron
AAATATTGCGGCCACCAATG+TGG	0.649069	5_4:-28833586	None:intergenic
AACACTCCAACATGTTCTTC+AGG	0.394266	5_4:+28830500	Msa0847250:five_prime_UTR
AACGTTTCTGCTTTACGACA+AGG	0.583533	5_4:+28830450	Msa0847250:five_prime_UTR
AAGACAAGACTGCACGTGAA+GGG	0.583181	5_4:-28830477	None:intergenic
AAGGAGAGTACTGATAGATG+AGG	0.645637	5_4:-28832772	None:intergenic
AAGTAAATATGGTAGTTCAA+CGG	0.547346	5_4:+28833172	Msa0847250:CDS
AATAGTCGAAGATCTCTAGA+AGG	0.505041	5_4:-28831512	None:intergenic
AATCGCAGGGCTGGTATAAA+AGG	0.334220	5_4:+28831380	Msa0847250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTACTCATACTTCTTATTGA+AGG	+	chr5_4:28830941-28830960	Msa0847250:intron	30.0%
TTCGTAGAAGTTAAAGAGTT+GGG	+	chr5_4:28831079-28831098	Msa0847250:intron	30.0%
GAGAACAATATACAATGCAA+TGG	+	chr5_4:28831295-28831314	Msa0847250:exon	30.0%
GTCATGGATAAAAATTGATC+AGG	+	chr5_4:28831430-28831449	Msa0847250:CDS	30.0%
TGGATAAAAATTGATCAGGA+TGG	+	chr5_4:28831434-28831453	Msa0847250:CDS	30.0%
GGATAAAAATTGATCAGGAT+GGG	+	chr5_4:28831435-28831454	Msa0847250:CDS	30.0%
ACAGGACAATATTATGCAAT+CGG	+	chr5_4:28831672-28831691	Msa0847250:CDS	30.0%
TACTCCATATAATTGCTTAC+TGG	+	chr5_4:28832000-28832019	Msa0847250:intron	30.0%
GAAACCAGTAAGCAATTATA+TGG	-	chr5_4:28832007-28832026	None:intergenic	30.0%
ATGATCTTCCCTTTGAATTT+AGG	+	chr5_4:28832040-28832059	Msa0847250:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	28830356	28834108	28830356	ID=Msa0847250;Name=Msa0847250
chr5_4	mRNA	28830356	28834108	28830356	ID=Msa0847250-mRNA-1;Parent=Msa0847250;Name=Msa0847250-mRNA-1;_AED=0.10;_eAED=0.10;_QI=267\|1\|1\|1\|1\|1\|7\|341\|405
chr5_4	exon	28830356	28830521	28830356	ID=Msa0847250-mRNA-1:exon:10811;Parent=Msa0847250-mRNA-1
chr5_4	exon	28831213	28831713	28831213	ID=Msa0847250-mRNA-1:exon:10812;Parent=Msa0847250-mRNA-1
chr5_4	exon	28832040	28832110	28832040	ID=Msa0847250-mRNA-1:exon:10813;Parent=Msa0847250-mRNA-1
chr5_4	exon	28832726	28832854	28832726	ID=Msa0847250-mRNA-1:exon:10814;Parent=Msa0847250-mRNA-1
chr5_4	exon	28832951	28833289	28832951	ID=Msa0847250-mRNA-1:exon:10815;Parent=Msa0847250-mRNA-1
chr5_4	exon	28833381	28833428	28833381	ID=Msa0847250-mRNA-1:exon:10816;Parent=Msa0847250-mRNA-1
chr5_4	exon	28833537	28834108	28833537	ID=Msa0847250-mRNA-1:exon:10817;Parent=Msa0847250-mRNA-1
chr5_4	five_prime_UTR	28830356	28830521	28830356	ID=Msa0847250-mRNA-1:five_prime_utr;Parent=Msa0847250-mRNA-1
chr5_4	five_prime_UTR	28831213	28831313	28831213	ID=Msa0847250-mRNA-1:five_prime_utr;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28831314	28831713	28831314	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28832040	28832110	28832040	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28832726	28832854	28832726	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28832951	28833289	28832951	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28833381	28833428	28833381	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	CDS	28833537	28833767	28833537	ID=Msa0847250-mRNA-1:cds;Parent=Msa0847250-mRNA-1
chr5_4	three_prime_UTR	28833768	28834108	28833768	ID=Msa0847250-mRNA-1:three_prime_utr;Parent=Msa0847250-mRNA-1

Gene Sequence

>Msa0847250

ATGGAAGAAACACAAGGGCAGTATTATGTTTCCAGTCTACATGAGTCTGATTTTAGCAGGCAAGCGAATCGCAGGGCTGGTATAAAAGGCCTGAAGCAGAGAGGTCATGGAAGTCGGTCATGGATAAAAATTGATCAGGATGGGAATTCCGAGATTGTGACACTTGACAAGGCTACGATTATGAGACATTGTTCTTTGCCTTCTAGAGATCTTCGACTATTGGATCCGAAATTCATTTATCCTTCTTCCATATTAGGAAGGGAGATGGCTATTGTTGTCAACCTTGAACAAATCCGGTGTATAATCACTGCCGATGAAGTCATCGTCATGAATTCACTCGATGGGACTGTTGGTCGGTACAGGACAATATTATGCAATCGGCTCCAGAGAGAAAAATCTGATGATCTTCCCTTTGAATTTAGGGCACTGGAGTTAGCTCTGGAATTGACATGCACATCTTTAGATGCTCAGGTAAATGAACTGGAGATGGAAATATATCCCGTGCTAGATGAACTGGCCTCATCTATCAGTACTCTCCTTCTGGAACGTGTTCGAAGATTTAAAGGTCATCTCCTTGCTTTGACTCAGCGAGTTCAAAAGGTCCGTGATGAAATAGAACATCTCATGGATGACGATGGTGACATGGCTGAGATGTGTCTGACTGAGAAAAGGAGAAGACTAGATGCTTCTCCTTCAAATGATTGTTTTCAAATTCGTTCACCATCAGGTAGAGTGATTTCAAAATCAGCCCCTACTTCACCAGAGCGGTCACTTAGTGGGCTTCAGATGTTGCCAAGGACTTTCAGCGGCATTGGAAATTCAAGTAAATATGGTAGTTCAACGGGTTCATCTGATAACACAGAAAGGATTCAGCCGCTGGAAATGTTGCTGGAAGCGTACTTTATCGTCATTGATAATACTCTCAACACATTATCGTCGCTCAAAGAATACATCGACGACACGGAAGATTTTCTCAACATAAAATTGGGAAATATTCAAAACCTCCTGATAAAGTTTGAGATGCTTCTTACAGCAGCCACATTGGTGGCCGCAATATTTGCCGCCGTGGCAGGAGTATTTGGAATGAACTTTGAAACCTCAGTTTTTGACTACTCTTCTGGATTCAATTTGGTTTTGGTAGTTACTGGAATTGGTTGTGTAGCATTGTATTTCGCCTTACTATTCTATTTTAGATACAAAAAAGTGCTTCCAGAATGA

Protein sequence

>Msa0847250

MEETQGQYYVSSLHESDFSRQANRRAGIKGLKQRGHGSRSWIKIDQDGNSEIVTLDKATIMRHCSLPSRDLRLLDPKFIYPSSILGREMAIVVNLEQIRCIITADEVIVMNSLDGTVGRYRTILCNRLQREKSDDLPFEFRALELALELTCTSLDAQVNELEMEIYPVLDELASSISTLLLERVRRFKGHLLALTQRVQKVRDEIEHLMDDDGDMAEMCLTEKRRRLDASPSNDCFQIRSPSGRVISKSAPTSPERSLSGLQMLPRTFSGIGNSSKYGSSTGSSDNTERIQPLEMLLEAYFIVIDNTLNTLSSLKEYIDDTEDFLNIKLGNIQNLLIKFEMLLTAATLVAAIFAAVAGVFGMNFETSVFDYSSGFNLVLVVTGIGCVALYFALLFYFRYKKVLPE*