AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0868300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868300 MtrunA17_Chr6g0488101 86.593 455 55 3 1 449 1 455 0.0 810
Msa0868300 MtrunA17_Chr6g0488271 86.265 415 54 2 38 449 1 415 0.0 743
Msa0868300 MtrunA17_Chr3g0112311 67.514 354 114 1 95 447 2 355 3.59e-172 487
Msa0868300 MtrunA17_Chr4g0075971 52.349 447 189 9 8 449 175 602 1.02e-145 428
Msa0868300 MtrunA17_Chr6g0452641 65.517 319 104 4 136 449 23 340 1.69e-143 413
Msa0868300 MtrunA17_Chr3g0077901 58.127 363 140 4 90 448 17 371 8.49e-142 410
Msa0868300 MtrunA17_Chr3g0077801 54.342 357 151 4 97 449 4 352 1.03e-131 384
Msa0868300 MtrunA17_Chr3g0077851 57.143 343 135 4 110 448 1 335 2.28e-129 377
Msa0868300 MtrunA17_Chr3g0122991 56.037 323 82 4 96 407 2 275 1.63e-114 337
Msa0868300 MtrunA17_Chr7g0252101 46.667 435 190 13 23 449 32 432 4.28e-111 334
Msa0868300 MtrunA17_Chr3g0093911 51.630 368 146 10 95 449 2 350 2.99e-106 319
Msa0868300 MtrunA17_Chr1g0207351 50.000 364 157 8 98 449 16 366 4.87e-106 319
Msa0868300 MtrunA17_Chr3g0123471 60.595 269 95 4 174 441 1 259 1.52e-103 308
Msa0868300 MtrunA17_Chr7g0243331 48.451 355 169 7 99 449 6 350 5.53e-100 303
Msa0868300 MtrunA17_Chr6g0486591 84.906 159 23 1 292 449 1 159 9.19e-95 282
Msa0868300 MtrunA17_Chr1g0209051 49.466 281 125 5 174 448 1 270 8.94e-79 245
Msa0868300 MtrunA17_Chr7g0243391 49.533 214 102 4 90 299 14 225 1.63e-52 176
Msa0868300 MtrunA17_Chr4g0075861 50.980 204 89 5 8 209 175 369 3.32e-52 180
Msa0868300 MtrunA17_Chr1g0202841 46.703 182 85 4 271 449 7 179 8.17e-44 151
Msa0868300 MtrunA17_Chr1g0196231 52.941 153 66 3 85 235 218 366 1.59e-42 154
Msa0868300 MtrunA17_Chr1g0206951 59.292 113 44 2 97 207 2 114 9.11e-37 131
Msa0868300 MtrunA17_Chr6g0486601 66.038 106 21 3 1 91 1 106 1.66e-34 124
Msa0868300 MtrunA17_Chr1g0187941 47.967 123 59 2 271 390 7 127 4.99e-26 102
Msa0868300 MtrunA17_Chr7g0238781 42.537 134 74 3 126 258 27 158 1.15e-25 102
Msa0868300 MtrunA17_Chr1g0209191 46.535 101 48 3 349 447 1 97 7.13e-21 87.0
Msa0868300 MtrunA17_Chr1g0196241 50.575 87 35 3 340 424 3 83 4.56e-20 84.7
Msa0868300 MtrunA17_Chr6g0459061 25.000 288 210 3 106 391 22 305 5.83e-20 90.9
Msa0868300 MtrunA17_Chr1g0209061 39.062 128 47 3 6 133 11 107 2.47e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGCGTAATCGTTTAATTT+TGG 0.114594 6_1:-1802984 Msa0868300:CDS
TCGGATCTGAATCTGAATTT+TGG 0.148836 6_1:-1803267 Msa0868300:five_prime_UTR
AGCATATTTCTTCTTGTAAA+TGG 0.163180 6_1:+1801581 None:intergenic
TCCTTTGATTTGCGAATAAA+TGG 0.190240 6_1:-1800795 Msa0868300:three_prime_UTR
ATGGCAAACGAACATGAAAT+TGG 0.218869 6_1:-1803163 Msa0868300:CDS
TCTTTGTTGGATTGCTTATT+CGG 0.232701 6_1:-1801417 Msa0868300:CDS
TTTGATGACCTCTTTCAATT+TGG 0.238893 6_1:-1801845 Msa0868300:CDS
TTATGTCAAGTTGGAATCTT+TGG 0.283864 6_1:-1801144 Msa0868300:CDS
GACAAATTCCTCTGAGCCTT+TGG 0.284348 6_1:+1801493 None:intergenic
ATCAATCTCAAAATCTTTGT+TGG 0.303941 6_1:-1801430 Msa0868300:CDS
TGCTTATTATTCTACACTTT+GGG 0.305214 6_1:-1800841 Msa0868300:three_prime_UTR
AGGATGAATAAGGTACTTAA+AGG 0.337066 6_1:-1803004 Msa0868300:CDS
TTTCAAAGAAAGGATGAATA+AGG 0.348934 6_1:-1803014 Msa0868300:CDS
ATGCCAGAATTCATTCTTCC+TGG 0.356806 6_1:-1800978 Msa0868300:CDS
TGACCAACTCGTTGTCGAAT+TGG 0.360565 6_1:+1802923 None:intergenic
AATTTATTGGAGGAATTTCA+TGG 0.361680 6_1:-1801789 Msa0868300:CDS
TACTTCCGATTTGCCTATCA+TGG 0.378106 6_1:-1801662 Msa0868300:CDS
CTTTGTTGGATTGCTTATTC+GGG 0.379417 6_1:-1801416 Msa0868300:CDS
CTCAGAGGAATTTGTCTTGG+TGG 0.385475 6_1:-1801486 Msa0868300:CDS
ATGCTTATTATTCTACACTT+TGG 0.386843 6_1:-1800842 Msa0868300:three_prime_UTR
ATGTTCCATGTGGCAAATAC+AGG 0.390944 6_1:-1801025 Msa0868300:CDS
TGCTTATTCGGGACCACATA+AGG 0.394621 6_1:-1801405 Msa0868300:CDS
ATTTCTCAAGAGCGCACCTT+TGG 0.401502 6_1:+1801744 None:intergenic
CAAAACGACGTTACGTAGAT+TGG 0.402196 6_1:-1800914 Msa0868300:CDS
GGCAAATACAGGGTGTATAA+AGG 0.407440 6_1:-1801014 Msa0868300:CDS
CAGTTCAAAATTTCAAAGAA+AGG 0.407992 6_1:-1803024 Msa0868300:CDS
AAGAGAACTGTCAACTTCCT+TGG 0.409046 6_1:-1801901 Msa0868300:CDS
TATGTCAAGTTGGAATCTTT+GGG 0.417393 6_1:-1801143 Msa0868300:CDS
GATGACCTCTTTCAATTTGG+TGG 0.428951 6_1:-1801842 Msa0868300:CDS
TTGGACAAGTAATGGTTCTT+CGG 0.433887 6_1:+1801763 None:intergenic
ACACCAGGAAGAATGAATTC+TGG 0.440652 6_1:+1800975 None:intergenic
CGAACATGAAATTGGGTTTG+TGG 0.442670 6_1:-1803155 Msa0868300:CDS
AGAGAACTGTCAACTTCCTT+GGG 0.449563 6_1:-1801900 Msa0868300:CDS
CTGGTGTAACTTCTAATTGT+TGG 0.451842 6_1:-1801268 Msa0868300:CDS
TATTATTCTACACTTTGGGT+TGG 0.458520 6_1:-1800837 Msa0868300:three_prime_UTR
AATCTGTGTTTCAAGGTAAG+AGG 0.459600 6_1:-1803123 Msa0868300:CDS
GCATTTCTTGTTGTGGATTG+TGG 0.473401 6_1:-1800671 Msa0868300:three_prime_UTR
TGGCAAACGAACATGAAATT+GGG 0.479747 6_1:-1803162 Msa0868300:CDS
CACTGAGCAACCCGCGCATG+TGG 0.492803 6_1:-1801079 Msa0868300:CDS
TGATTTGCGAATAAATGGAC+AGG 0.494531 6_1:-1800790 Msa0868300:three_prime_UTR
TCCATTTATTCGCAAATCAA+AGG 0.494697 6_1:+1800794 None:intergenic
AGTGCTTAATATTATGTCAA+AGG 0.495744 6_1:-1801207 Msa0868300:CDS
GGATGAATAAGGTACTTAAA+GGG 0.501407 6_1:-1803003 Msa0868300:CDS
AATTTCATGTTCGTTTGCCA+TGG 0.506447 6_1:+1803165 None:intergenic
GTAGGGTTGGTTAGTTGTGA+TGG 0.506721 6_1:-1801239 Msa0868300:CDS
CTAATTGTTGGTCACACGTA+GGG 0.509336 6_1:-1801256 Msa0868300:CDS
AGTTGTAGCATTTCTTGTTG+TGG 0.514265 6_1:-1800678 Msa0868300:three_prime_UTR
AAGACCCATGATAGGCAAAT+CGG 0.519986 6_1:+1801657 None:intergenic
TCCGCTAGAAGACCCATGAT+AGG 0.521743 6_1:+1801649 None:intergenic
ATCTGTGTTTCAAGGTAAGA+GGG 0.522269 6_1:-1803122 Msa0868300:CDS
CGGCTTCTTCAATTGAGGAT+CGG 0.524330 6_1:-1803238 Msa0868300:five_prime_UTR
TACCATGGATTATGTCAAGT+TGG 0.524896 6_1:-1801153 Msa0868300:CDS
CAAGCTGAGTATCCCTATGT+TGG 0.527658 6_1:+1801331 None:intergenic
GCGCACCTTTGGACAAGTAA+TGG 0.527847 6_1:+1801755 None:intergenic
AGGCTCAGAGGAATTTGTCT+TGG 0.528013 6_1:-1801489 Msa0868300:CDS
AGCTAAGCAAGCAGCCTCCA+TGG 0.530098 6_1:-1803182 Msa0868300:exon
TTAGAACTGAAGAATACTCC+TGG 0.536982 6_1:-1801287 Msa0868300:CDS
GAAGTAAAATCTGTGTTTCA+AGG 0.545805 6_1:-1803130 Msa0868300:CDS
CAACGAGTTGGTCATAGTAA+TGG 0.547773 6_1:-1802914 Msa0868300:intron
AAATGCTACAACTACAAGCT+AGG 0.548587 6_1:+1800688 None:intergenic
GAGCGGCAATAACTAGGATG+TGG 0.552636 6_1:+1800936 None:intergenic
TTCTCATAACCCCACATGCG+CGG 0.556155 6_1:+1801068 None:intergenic
GCCTATCATGGGTCTTCTAG+CGG 0.557470 6_1:-1801650 Msa0868300:CDS
GTCACTGACAAGGCCAACAT+AGG 0.560597 6_1:-1801344 Msa0868300:CDS
TCTCATAACCCCACATGCGC+GGG 0.566108 6_1:+1801069 None:intergenic
CAACAACACAACACACGCAT+CGG 0.567355 6_1:-1803286 None:intergenic
GCAAAGTCAACAACCTTATG+TGG 0.569183 6_1:+1801392 None:intergenic
CTGAGCAACCCGCGCATGTG+GGG 0.569892 6_1:-1801077 Msa0868300:CDS
TTAGTCGGCTTCTTCAATTG+AGG 0.573899 6_1:-1803243 Msa0868300:five_prime_UTR
TACACCCTGTATTTGCCACA+TGG 0.574023 6_1:+1801020 None:intergenic
TTCATGTTCGTTTGCCATGG+AGG 0.575112 6_1:+1803168 None:intergenic
ACTTCCGATTTGCCTATCAT+GGG 0.586293 6_1:-1801661 Msa0868300:CDS
GAGTGATAAACTTTATCGTG+AGG 0.586533 6_1:+1801701 None:intergenic
ATATTTCATCATGACACGAA+TGG 0.586960 6_1:-1801627 Msa0868300:CDS
ACTGAGCAACCCGCGCATGT+GGG 0.589599 6_1:-1801078 Msa0868300:CDS
AGTTCACTGCGTGTAACACC+AGG 0.590897 6_1:+1800960 None:intergenic
TCACTGACAAGGCCAACATA+GGG 0.593942 6_1:-1801343 Msa0868300:CDS
ATACACCACCAAATTGAAAG+AGG 0.598201 6_1:+1801837 None:intergenic
CGTATATGTTGTCACTGACA+AGG 0.598708 6_1:-1801354 Msa0868300:CDS
TTGTGGATTGTGGCACATTG+AGG 0.599891 6_1:-1800661 Msa0868300:three_prime_UTR
TTCCAACTTGACATAATCCA+TGG 0.602044 6_1:+1801151 None:intergenic
CTTCCAATTCGACAACGAGT+TGG 0.607277 6_1:-1802926 Msa0868300:CDS
TGTTCCATGTGGCAAATACA+GGG 0.612242 6_1:-1801024 Msa0868300:CDS
TTGTTGGTCACACGTAGGGT+TGG 0.615794 6_1:-1801252 Msa0868300:CDS
ACATGAAATTGGGTTTGTGG+AGG 0.626424 6_1:-1803152 Msa0868300:CDS
CAACAATTAGAAGTTACACC+AGG 0.627295 6_1:+1801269 None:intergenic
CAAGATAGACTTCTCTACCA+TGG 0.632047 6_1:-1801168 Msa0868300:CDS
AAGAACCATTACTTGTCCAA+AGG 0.641369 6_1:-1801760 Msa0868300:CDS
AATACGACGATGCTTCATGT+AGG 0.643363 6_1:+1802952 None:intergenic
AGTGCATAATACTTTCTCGT+CGG 0.645156 6_1:+1801098 None:intergenic
TCTAATTGTTGGTCACACGT+AGG 0.652885 6_1:-1801257 Msa0868300:CDS
ATGATGTCAAGCAAATCCCA+AGG 0.658074 6_1:+1801884 None:intergenic
TTTGCCACATGGAACATCGA+GGG 0.658254 6_1:+1801031 None:intergenic
ATTTGCCACATGGAACATCG+AGG 0.704595 6_1:+1801030 None:intergenic
TATGCCCTCGATGTTCCATG+TGG 0.716778 6_1:-1801035 Msa0868300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAACATAAAAAAAATA+AGG - chr6_1:1801797-1801816 Msa0868300:CDS 10.0%
!! ATACATAATTATTTATATGT+TGG - chr6_1:1801823-1801842 Msa0868300:CDS 10.0%
!!! GAGAATTTTATAAAATTTAT+TGG - chr6_1:1802135-1802154 Msa0868300:intron 10.0%
!!! AATTTTATAAAATTTATTGG+AGG - chr6_1:1802138-1802157 Msa0868300:intron 10.0%
!!! AATCTGATATATGTATGTTT+TGG - chr6_1:1801138-1801157 Msa0868300:CDS 20.0%
!!! ATCTGATATATGTATGTTTT+GGG - chr6_1:1801139-1801158 Msa0868300:CDS 20.0%
!!! CTGGTGTTATTATATATTTT+AGG - chr6_1:1801180-1801199 Msa0868300:CDS 20.0%
!! TGGTGTTTGTTTAAATATAA+GGG - chr6_1:1801249-1801268 Msa0868300:CDS 20.0%
!!! TATATTATTTGTTTGTTGCT+AGG - chr6_1:1801279-1801298 Msa0868300:CDS 20.0%
!!! AAAATATTTTTCTGTTCTTG+CGG + chr6_1:1801630-1801649 None:intergenic 20.0%
!!! AGAACAGAAAAATATTTTGT+AGG - chr6_1:1801632-1801651 Msa0868300:CDS 20.0%
!!! CTCATTTTCTATGATATTAT+GGG - chr6_1:1801894-1801913 Msa0868300:CDS 20.0%
!!! ATGGTTTACAGTTTTATTAT+TGG - chr6_1:1803186-1803205 Msa0868300:five_prime_UTR 20.0%
!!! AATCTGAATTTTGGATTAGT+CGG - chr6_1:1800679-1800698 Msa0868300:three_prime_UTR 25.0%
!!! CTTTCTTTGAAATTTTGAAC+TGG + chr6_1:1800915-1800934 None:intergenic 25.0%
! CAGTTCAAAATTTCAAAGAA+AGG - chr6_1:1800913-1800932 Msa0868300:CDS 25.0%
! TTTCAAAGAAAGGATGAATA+AGG - chr6_1:1800923-1800942 Msa0868300:CDS 25.0%
!! TCTGATATATGTATGTTTTG+GGG - chr6_1:1801140-1801159 Msa0868300:CDS 25.0%
!!! ATATATTTTAGGATACACCA+AGG - chr6_1:1801191-1801210 Msa0868300:CDS 25.0%
! TCAATTTCTCCAACAAATAA+CGG - chr6_1:1801224-1801243 Msa0868300:CDS 25.0%
! CTGGTGTTTGTTTAAATATA+AGG - chr6_1:1801248-1801267 Msa0868300:CDS 25.0%
! GTTTGTTTAAATATAAGGGA+AGG - chr6_1:1801253-1801272 Msa0868300:CDS 25.0%
!! CAGAAAAATATTTTGTAGGT+AGG - chr6_1:1801636-1801655 Msa0868300:CDS 25.0%
!! AAATATTTTGTAGGTAGGAT+TGG - chr6_1:1801641-1801660 Msa0868300:CDS 25.0%
! GAAATTAAACTACAACAACT+AGG - chr6_1:1801749-1801768 Msa0868300:CDS 25.0%
! GATATTTGTTTCGAAAACAT+GGG - chr6_1:1801850-1801869 Msa0868300:CDS 25.0%
! CCATAATATCATAGAAAATG+AGG + chr6_1:1801896-1801915 None:intergenic 25.0%
!! CCTCATTTTCTATGATATTA+TGG - chr6_1:1801893-1801912 Msa0868300:CDS 25.0%
! AATTTATTGGAGGAATTTCA+TGG - chr6_1:1802148-1802167 Msa0868300:intron 25.0%
! AGCATATTTCTTCTTGTAAA+TGG + chr6_1:1802359-1802378 None:intergenic 25.0%
! ATCAATCTCAAAATCTTTGT+TGG - chr6_1:1802507-1802526 Msa0868300:intron 25.0%
! AGTGCTTAATATTATGTCAA+AGG - chr6_1:1802730-1802749 Msa0868300:intron 25.0%
!! TTTTAGACATCTACAATATG+AGG - chr6_1:1803048-1803067 Msa0868300:CDS 25.0%
! ATGCTTATTATTCTACACTT+TGG - chr6_1:1803095-1803114 Msa0868300:CDS 25.0%
! TGCTTATTATTCTACACTTT+GGG - chr6_1:1803096-1803115 Msa0868300:CDS 25.0%
! AAGGAAAAACAAATAGAACA+AGG + chr6_1:1803127-1803146 None:intergenic 25.0%
! AGGATTGAAAAATGTTGTAA+TGG - chr6_1:1803167-1803186 Msa0868300:exon 25.0%
GAAGTAAAATCTGTGTTTCA+AGG - chr6_1:1800807-1800826 Msa0868300:three_prime_UTR 30.0%
! AGGATGAATAAGGTACTTAA+AGG - chr6_1:1800933-1800952 Msa0868300:CDS 30.0%
! GGATGAATAAGGTACTTAAA+GGG - chr6_1:1800934-1800953 Msa0868300:CDS 30.0%
!! CTGATATATGTATGTTTTGG+GGG - chr6_1:1801141-1801160 Msa0868300:CDS 30.0%
! TATTTGTTTGTTGCTAGGTA+TGG - chr6_1:1801284-1801303 Msa0868300:CDS 30.0%
TGTTAGTAGATTTCTTAGGT+TGG + chr6_1:1801487-1801506 None:intergenic 30.0%
CATGTGTTAGTAGATTTCTT+AGG + chr6_1:1801491-1801510 None:intergenic 30.0%
TGTAAGTAGATGGAATGATA+GGG + chr6_1:1801688-1801707 None:intergenic 30.0%
TATCATTCCATCTACTTACA+GGG - chr6_1:1801688-1801707 Msa0868300:CDS 30.0%
ATTCATTGCAAGCTATAACT+AGG - chr6_1:1801718-1801737 Msa0868300:CDS 30.0%
CGATATTTGTTTCGAAAACA+TGG - chr6_1:1801849-1801868 Msa0868300:CDS 30.0%
CAAAGGACAAAAAAAGAAGA+AGG - chr6_1:1802010-1802029 Msa0868300:intron 30.0%
! ATTTGCTTGACATCATTTTG+AGG - chr6_1:1802060-1802079 Msa0868300:intron 30.0%
! TTTGATGACCTCTTTCAATT+TGG - chr6_1:1802092-1802111 Msa0868300:intron 30.0%
ATATTTCATCATGACACGAA+TGG - chr6_1:1802310-1802329 Msa0868300:intron 30.0%
TCTTTGTTGGATTGCTTATT+CGG - chr6_1:1802520-1802539 Msa0868300:intron 30.0%
!! TTATGTCAAGTTGGAATCTT+TGG - chr6_1:1802793-1802812 Msa0868300:intron 30.0%
!! TATGTCAAGTTGGAATCTTT+GGG - chr6_1:1802794-1802813 Msa0868300:intron 30.0%
TATTATTCTACACTTTGGGT+TGG - chr6_1:1803100-1803119 Msa0868300:CDS 30.0%
!! TCCTTTGATTTGCGAATAAA+TGG - chr6_1:1803142-1803161 Msa0868300:CDS 30.0%
TCCATTTATTCGCAAATCAA+AGG + chr6_1:1803146-1803165 None:intergenic 30.0%
!!! CAAGTTTTGAGATAGTATAG+AGG - chr6_1:1803210-1803229 Msa0868300:five_prime_UTR 30.0%
! TCGGATCTGAATCTGAATTT+TGG - chr6_1:1800670-1800689 Msa0868300:three_prime_UTR 35.0%
AATTTCATGTTCGTTTGCCA+TGG + chr6_1:1800775-1800794 None:intergenic 35.0%
ATGGCAAACGAACATGAAAT+TGG - chr6_1:1800774-1800793 Msa0868300:three_prime_UTR 35.0%
TGGCAAACGAACATGAAATT+GGG - chr6_1:1800775-1800794 Msa0868300:three_prime_UTR 35.0%
AATCTGTGTTTCAAGGTAAG+AGG - chr6_1:1800814-1800833 Msa0868300:three_prime_UTR 35.0%
ATCTGTGTTTCAAGGTAAGA+GGG - chr6_1:1800815-1800834 Msa0868300:three_prime_UTR 35.0%
! GAAATTTTGAACTGGAAGTC+TGG + chr6_1:1800907-1800926 None:intergenic 35.0%
!! AGGGCGTAATCGTTTAATTT+TGG - chr6_1:1800953-1800972 Msa0868300:CDS 35.0%
!! GGGCGTAATCGTTTAATTTT+GGG - chr6_1:1800954-1800973 Msa0868300:CDS 35.0%
AAATTGAACTCAACTCTCCT+TGG + chr6_1:1801211-1801230 None:intergenic 35.0%
TTTGTTGCTAGGTATGGTAA+TGG - chr6_1:1801290-1801309 Msa0868300:CDS 35.0%
TTACACTAACTCACTCATGT+AGG + chr6_1:1801324-1801343 None:intergenic 35.0%
TCGGCCATGAAAATAAACAT+AGG - chr6_1:1801361-1801380 Msa0868300:CDS 35.0%
GTTCATTCATATTGTGACAG+TGG + chr6_1:1801407-1801426 None:intergenic 35.0%
TCACAATATGAATGAACAGC+AGG - chr6_1:1801410-1801429 Msa0868300:CDS 35.0%
GGCTGCTTAATTGTATATGT+AGG + chr6_1:1801466-1801485 None:intergenic 35.0%
CTGTAAGTAGATGGAATGAT+AGG + chr6_1:1801689-1801708 None:intergenic 35.0%
CTATCATTCCATCTACTTAC+AGG - chr6_1:1801687-1801706 Msa0868300:CDS 35.0%
! TATAGCTTGCAATGAATAGC+CGG + chr6_1:1801715-1801734 None:intergenic 35.0%
ATTGCAAGCTATAACTAGGT+AGG - chr6_1:1801722-1801741 Msa0868300:CDS 35.0%
AAGCTATAACTAGGTAGGAT+TGG - chr6_1:1801727-1801746 Msa0868300:CDS 35.0%
TTAAACTACAACAACTAGGC+AGG - chr6_1:1801753-1801772 Msa0868300:CDS 35.0%
ATACACCACCAAATTGAAAG+AGG + chr6_1:1802103-1802122 None:intergenic 35.0%
!! TTGGTGGTGTATGCAAAAAT+TGG - chr6_1:1802111-1802130 Msa0868300:intron 35.0%
TTGGACAAGTAATGGTTCTT+CGG + chr6_1:1802177-1802196 None:intergenic 35.0%
AAGAACCATTACTTGTCCAA+AGG - chr6_1:1802177-1802196 Msa0868300:intron 35.0%
GAGTGATAAACTTTATCGTG+AGG + chr6_1:1802239-1802258 None:intergenic 35.0%
CTTTGTTGGATTGCTTATTC+GGG - chr6_1:1802521-1802540 Msa0868300:intron 35.0%
TTAGAACTGAAGAATACTCC+TGG - chr6_1:1802650-1802669 Msa0868300:intron 35.0%
CAACAATTAGAAGTTACACC+AGG + chr6_1:1802671-1802690 None:intergenic 35.0%
CTGGTGTAACTTCTAATTGT+TGG - chr6_1:1802669-1802688 Msa0868300:intron 35.0%
! TACCATGGATTATGTCAAGT+TGG - chr6_1:1802784-1802803 Msa0868300:intron 35.0%
TTCCAACTTGACATAATCCA+TGG + chr6_1:1802789-1802808 None:intergenic 35.0%
AGTGCATAATACTTTCTCGT+CGG + chr6_1:1802842-1802861 None:intergenic 35.0%
! TGATTTGCGAATAAATGGAC+AGG - chr6_1:1803147-1803166 Msa0868300:CDS 35.0%
AAATGCTACAACTACAAGCT+AGG + chr6_1:1803252-1803271 None:intergenic 35.0%
AGTTGTAGCATTTCTTGTTG+TGG - chr6_1:1803259-1803278 Msa0868300:five_prime_UTR 35.0%
! TTAGTCGGCTTCTTCAATTG+AGG - chr6_1:1800694-1800713 Msa0868300:three_prime_UTR 40.0%
CGAACATGAAATTGGGTTTG+TGG - chr6_1:1800782-1800801 Msa0868300:three_prime_UTR 40.0%
ACATGAAATTGGGTTTGTGG+AGG - chr6_1:1800785-1800804 Msa0868300:three_prime_UTR 40.0%
AATACGACGATGCTTCATGT+AGG + chr6_1:1800988-1801007 None:intergenic 40.0%
!! CAACGAGTTGGTCATAGTAA+TGG - chr6_1:1801023-1801042 Msa0868300:CDS 40.0%
TCAGATTCTCTATTCGACCT+GGG + chr6_1:1801125-1801144 None:intergenic 40.0%
ATCAGATTCTCTATTCGACC+TGG + chr6_1:1801126-1801145 None:intergenic 40.0%
GGGAAAATTTGACTCACTTC+TGG - chr6_1:1801161-1801180 Msa0868300:CDS 40.0%
TTCTCCAACAAATAACGGTC+TGG - chr6_1:1801229-1801248 Msa0868300:CDS 40.0%
AACACCAGACCGTTATTTGT+TGG + chr6_1:1801236-1801255 None:intergenic 40.0%
AAGACACACTAACACACTCT+CGG - chr6_1:1801342-1801361 Msa0868300:CDS 40.0%
!! GGCGCCTATGTTTATTTTCA+TGG + chr6_1:1801368-1801387 None:intergenic 40.0%
AAACATAGGCGCCTTGTAAT+CGG - chr6_1:1801375-1801394 Msa0868300:CDS 40.0%
! AGTGGTTAGTTCCGATTACA+AGG + chr6_1:1801389-1801408 None:intergenic 40.0%
ACCCAAAACCAAACACAATG+AGG - chr6_1:1801440-1801459 Msa0868300:CDS 40.0%
!!! GCCTCATTGTGTTTGGTTTT+GGG + chr6_1:1801444-1801463 None:intergenic 40.0%
TATGTAGGCCTCATTGTGTT+TGG + chr6_1:1801451-1801470 None:intergenic 40.0%
! GGTTTGGTTTCAAAGGCTTT+GGG + chr6_1:1801524-1801543 None:intergenic 40.0%
! TTCAGGAGGTTTTTGTTCTG+CGG + chr6_1:1801545-1801564 None:intergenic 40.0%
AAAAACCTCCTGAACAAAGC+TGG - chr6_1:1801551-1801570 Msa0868300:CDS 40.0%
AAAACCTCCTGAACAAAGCT+GGG - chr6_1:1801552-1801571 Msa0868300:CDS 40.0%
! TTTCTGTTCTTGCGGTTTTG+AGG + chr6_1:1801622-1801641 None:intergenic 40.0%
TCACAACAAACGACGATGTT+AGG + chr6_1:1801988-1802007 None:intergenic 40.0%
!! CGTCGTTTGTTGTGATACAA+AGG - chr6_1:1801993-1802012 Msa0868300:intron 40.0%
AAGAGAACTGTCAACTTCCT+TGG - chr6_1:1802036-1802055 Msa0868300:intron 40.0%
AGAGAACTGTCAACTTCCTT+GGG - chr6_1:1802037-1802056 Msa0868300:intron 40.0%
ATGATGTCAAGCAAATCCCA+AGG + chr6_1:1802056-1802075 None:intergenic 40.0%
GATGACCTCTTTCAATTTGG+TGG - chr6_1:1802095-1802114 Msa0868300:intron 40.0%
! TACTTCCGATTTGCCTATCA+TGG - chr6_1:1802275-1802294 Msa0868300:intron 40.0%
! ACTTCCGATTTGCCTATCAT+GGG - chr6_1:1802276-1802295 Msa0868300:intron 40.0%
AAGACCCATGATAGGCAAAT+CGG + chr6_1:1802283-1802302 None:intergenic 40.0%
!!! GTCTTGCTTGCTTTTTCCAA+AGG - chr6_1:1802428-1802447 Msa0868300:intron 40.0%
GCAAAGTCAACAACCTTATG+TGG + chr6_1:1802548-1802567 None:intergenic 40.0%
CGTATATGTTGTCACTGACA+AGG - chr6_1:1802583-1802602 Msa0868300:intron 40.0%
TCTAATTGTTGGTCACACGT+AGG - chr6_1:1802680-1802699 Msa0868300:intron 40.0%
CTAATTGTTGGTCACACGTA+GGG - chr6_1:1802681-1802700 Msa0868300:intron 40.0%
CAAGATAGACTTCTCTACCA+TGG - chr6_1:1802769-1802788 Msa0868300:intron 40.0%
ATGTTCCATGTGGCAAATAC+AGG - chr6_1:1802912-1802931 Msa0868300:intron 40.0%
TGTTCCATGTGGCAAATACA+GGG - chr6_1:1802913-1802932 Msa0868300:intron 40.0%
GGCAAATACAGGGTGTATAA+AGG - chr6_1:1802923-1802942 Msa0868300:CDS 40.0%
ATGCCAGAATTCATTCTTCC+TGG - chr6_1:1802959-1802978 Msa0868300:CDS 40.0%
ACACCAGGAAGAATGAATTC+TGG + chr6_1:1802965-1802984 None:intergenic 40.0%
CAAAACGACGTTACGTAGAT+TGG - chr6_1:1803023-1803042 Msa0868300:CDS 40.0%
! GCATTTCTTGTTGTGGATTG+TGG - chr6_1:1803266-1803285 Msa0868300:five_prime_UTR 40.0%
CGGCTTCTTCAATTGAGGAT+CGG - chr6_1:1800699-1800718 Msa0868300:three_prime_UTR 45.0%
TTCATGTTCGTTTGCCATGG+AGG + chr6_1:1800772-1800791 None:intergenic 45.0%
CTTCCAATTCGACAACGAGT+TGG - chr6_1:1801011-1801030 Msa0868300:CDS 45.0%
TGACCAACTCGTTGTCGAAT+TGG + chr6_1:1801017-1801036 None:intergenic 45.0%
TTATTTCACAAAACCCGCCC+AGG - chr6_1:1801105-1801124 Msa0868300:CDS 45.0%
ATGAATGAACAGCAGGCCAA+AGG - chr6_1:1801417-1801436 Msa0868300:CDS 45.0%
!!! GTGTTTGGTTTTGGGTCCTT+TGG + chr6_1:1801436-1801455 None:intergenic 45.0%
!! GGCCTCATTGTGTTTGGTTT+TGG + chr6_1:1801445-1801464 None:intergenic 45.0%
!! CGGTTTGGTTTCAAAGGCTT+TGG + chr6_1:1801525-1801544 None:intergenic 45.0%
!! GTTCTGCGGTTTGGTTTCAA+AGG + chr6_1:1801531-1801550 None:intergenic 45.0%
! GAGGTTTTTGTTCTGCGGTT+TGG + chr6_1:1801540-1801559 None:intergenic 45.0%
!!! GTTTTTGCCCAGCTTTGTTC+AGG + chr6_1:1801562-1801581 None:intergenic 45.0%
! GATTGGCATTGTGTTGCTGT+GGG + chr6_1:1801586-1801605 None:intergenic 45.0%
! TGATTGGCATTGTGTTGCTG+TGG + chr6_1:1801587-1801606 None:intergenic 45.0%
GAGGATTGCTTCAAGCTGAT+TGG + chr6_1:1801603-1801622 None:intergenic 45.0%
TTCCATCTACTTACAGGGAC+CGG - chr6_1:1801693-1801712 Msa0868300:CDS 45.0%
CTACAACAACTAGGCAGGAT+TGG - chr6_1:1801758-1801777 Msa0868300:CDS 45.0%
! ATTTCTCAAGAGCGCACCTT+TGG + chr6_1:1802196-1802215 None:intergenic 45.0%
!!! TGCTTTTTCCAAAGGCTCAG+AGG - chr6_1:1802436-1802455 Msa0868300:intron 45.0%
GACAAATTCCTCTGAGCCTT+TGG + chr6_1:1802447-1802466 None:intergenic 45.0%
AGGCTCAGAGGAATTTGTCT+TGG - chr6_1:1802448-1802467 Msa0868300:intron 45.0%
CTCAGAGGAATTTGTCTTGG+TGG - chr6_1:1802451-1802470 Msa0868300:intron 45.0%
TGCTTATTCGGGACCACATA+AGG - chr6_1:1802532-1802551 Msa0868300:intron 45.0%
TCACTGACAAGGCCAACATA+GGG - chr6_1:1802594-1802613 Msa0868300:intron 45.0%
CAAGCTGAGTATCCCTATGT+TGG + chr6_1:1802609-1802628 None:intergenic 45.0%
!! GTAGGGTTGGTTAGTTGTGA+TGG - chr6_1:1802698-1802717 Msa0868300:intron 45.0%
TTTGCCACATGGAACATCGA+GGG + chr6_1:1802909-1802928 None:intergenic 45.0%
ATTTGCCACATGGAACATCG+AGG + chr6_1:1802910-1802929 None:intergenic 45.0%
TACACCCTGTATTTGCCACA+TGG + chr6_1:1802920-1802939 None:intergenic 45.0%
!! CGTTTTGAGCGGCAATAACT+AGG + chr6_1:1803010-1803029 None:intergenic 45.0%
!! CTACGTAACGTCGTTTTGAG+CGG + chr6_1:1803021-1803040 None:intergenic 45.0%
! TTGTGGATTGTGGCACATTG+AGG - chr6_1:1803276-1803295 Msa0868300:five_prime_UTR 45.0%
!!! ATGATTTTTTATAATTATTA+TGG + chr6_1:1801921-1801940 None:intergenic 5.0%
ATTCTCTATTCGACCTGGGC+GGG + chr6_1:1801121-1801140 None:intergenic 50.0%
GATTCTCTATTCGACCTGGG+CGG + chr6_1:1801122-1801141 None:intergenic 50.0%
!! TTTGCCCAGCTTTGTTCAGG+AGG + chr6_1:1801559-1801578 None:intergenic 50.0%
GCGCACCTTTGGACAAGTAA+TGG + chr6_1:1802185-1802204 None:intergenic 50.0%
GCCTATCATGGGTCTTCTAG+CGG - chr6_1:1802287-1802306 Msa0868300:intron 50.0%
TCCGCTAGAAGACCCATGAT+AGG + chr6_1:1802291-1802310 None:intergenic 50.0%
GTCACTGACAAGGCCAACAT+AGG - chr6_1:1802593-1802612 Msa0868300:intron 50.0%
TTGTTGGTCACACGTAGGGT+TGG - chr6_1:1802685-1802704 Msa0868300:intron 50.0%
TTCTCATAACCCCACATGCG+CGG + chr6_1:1802872-1802891 None:intergenic 50.0%
TATGCCCTCGATGTTCCATG+TGG - chr6_1:1802902-1802921 Msa0868300:intron 50.0%
AGTTCACTGCGTGTAACACC+AGG + chr6_1:1802980-1802999 None:intergenic 50.0%
GAGCGGCAATAACTAGGATG+TGG + chr6_1:1803004-1803023 None:intergenic 50.0%
AGCTAAGCAAGCAGCCTCCA+TGG - chr6_1:1800755-1800774 Msa0868300:three_prime_UTR 55.0%
!! CAAAGGCTTTGGGTGATGCG+AGG + chr6_1:1801514-1801533 None:intergenic 55.0%
AGCCGGTCCCTGTAAGTAGA+TGG + chr6_1:1801698-1801717 None:intergenic 55.0%
TCTCATAACCCCACATGCGC+GGG + chr6_1:1802871-1802890 None:intergenic 55.0%
ACTGAGCAACCCGCGCATGT+GGG - chr6_1:1802859-1802878 Msa0868300:intron 60.0%
CACTGAGCAACCCGCGCATG+TGG - chr6_1:1802858-1802877 Msa0868300:intron 65.0%
CTGAGCAACCCGCGCATGTG+GGG - chr6_1:1802860-1802879 Msa0868300:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 1800659 1803300 1800659 ID=Msa0868300;Name=Msa0868300
chr6_1 mRNA 1800659 1803300 1800659 ID=Msa0868300-mRNA-1;Parent=Msa0868300;Name=Msa0868300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=115|1|1|1|1|1|2|207|449
chr6_1 exon 1800659 1801944 1800659 ID=Msa0868300-mRNA-1:exon:507;Parent=Msa0868300-mRNA-1
chr6_1 exon 1802915 1803300 1802915 ID=Msa0868300-mRNA-1:exon:506;Parent=Msa0868300-mRNA-1
chr6_1 five_prime_UTR 1803186 1803300 1803186 ID=Msa0868300-mRNA-1:five_prime_utr;Parent=Msa0868300-mRNA-1
chr6_1 CDS 1802915 1803185 1802915 ID=Msa0868300-mRNA-1:cds;Parent=Msa0868300-mRNA-1
chr6_1 CDS 1800866 1801944 1800866 ID=Msa0868300-mRNA-1:cds;Parent=Msa0868300-mRNA-1
chr6_1 three_prime_UTR 1800659 1800865 1800659 ID=Msa0868300-mRNA-1:three_prime_utr;Parent=Msa0868300-mRNA-1
Gene Sequence

>Msa0868300

ATGGCAAACGAACATGAAATTGGGTTTGTGGAGGAAGTAAAATCTGTGTTTCAAGGTAAGAGGGAAGAGTATGAAGAGTTTATAAAAGACATACATGCTTTTAAGACGCTGATGAATGATCATCGAATCACCAGACTTCCAGTTCAAAATTTCAAAGAAAGGATGAATAAGGTACTTAAAGGGCGTAATCGTTTAATTTTGGGATTCAACGCCTACATGAAGCATCGTCGTATTACACTTCCAATTCGACAACGAGTTGGTCATAGTAATGGACAAAAAAAGAAGAAGGTAGAAGAGAACTGTCAACTTCCTTGGGATTTGCTTGACATCATTTTGAGGAATCTAGATTTTGATGACCTCTTTCAATTTGGTGGTGTATGCAAAAATTGGCGAGAATTTTATAAAATTTATTGGAGGAATTTCATGGCATCCGAAGAACCATTACTTGTCCAAAGGTGCGCTCTTGAGAAATCTTTCAACTTCTTCAGCTTACCTCACGATAAAGTTTATCACTCAAAGATGATCAATAACTACTTCCGATTTGCCTATCATGGGTCTTCTAGCGGATATTTCATCATGACACGAATGGATAATTCATTTATACTTATTAATCCATTTACAAGAAGAAATATGCTAATCAATAACTCAGTCTTCAATGTTGATTTCAAACATTTTTCTTGCAAAGTCTTGCTTGCTTTTTCCAAAGGCTCAGAGGAATTTGTCTTGGTGGTTTTATGTATTAATTCTGATAATTTGCATGTCTATCAATCTCAAAATCTTTGTTGGATTGCTTATTCGGGACCACATAAGGTTGTTGACTTTGCTGTTTTAAATTATATCGTATATGTTGTCACTGACAAGGCCAACATAGGGATACTCAGCTTGAAATATGCAAATATAAATTTTTTAGAACTGAAGAATACTCCTGGTGTAACTTCTAATTGTTGGTCACACGTAGGGTTGGTTAGTTGTGATGGATATCTTTTAGTGCTTAATATTATGTCAAAGGTAACGTTTAACGTGTACAAGATAGACTTCTCTACCATGGATTATGTCAAGTTGGAATCTTTGGGTGACATTGCAATATTTCATCTTCCGACGAGAAAGTATTATGCACTGAGCAACCCGCGCATGTGGGGTTATGAGAACAATTCTATCTATGCCCTCGATGTTCCATGTGGCAAATACAGGGTGTATAAAGGAGATAACAATAAAATGCCAGAATTCATTCTTCCTGGTGTTACACGCAGTGAACTTCCACATCCTAGTTATTGCCGCTCAAAACGACGTTACGTAGATTGGTGTTTTAGACATCTACAATATGAGGTAGATTATACTCTTGCTGAGTAG
Protein sequence

>Msa0868300

MANEHEIGFVEEVKSVFQGKREEYEEFIKDIHAFKTLMNDHRITRLPVQNFKERMNKVLKGRNRLILGFNAYMKHRRITLPIRQRVGHSNGQKKKKVEENCQLPWDLLDIILRNLDFDDLFQFGGVCKNWREFYKIYWRNFMASEEPLLVQRCALEKSFNFFSLPHDKVYHSKMINNYFRFAYHGSSSGYFIMTRMDNSFILINPFTRRNMLINNSVFNVDFKHFSCKVLLAFSKGSEEFVLVVLCINSDNLHVYQSQNLCWIAYSGPHKVVDFAVLNYIVYVVTDKANIGILSLKYANINFLELKNTPGVTSNCWSHVGLVSCDGYLLVLNIMSKVTFNVYKIDFSTMDYVKLESLGDIAIFHLPTRKYYALSNPRMWGYENNSIYALDVPCGKYRVYKGDNNKMPEFILPGVTRSELPHPSYCRSKRRYVDWCFRHLQYEVDYTLAE*