AlfalfaGEDB | Msa0893330

M. sativa cultivar zhongmu-4 / Msa0893330

KEGG: K02992
GO: GO:0000028 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0016020 GO:0016043 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0034622 GO:0042254 GO:0042255 GO:0042274 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0070925 GO:0071826 GO:0071840 GO:0097159 GO:1901363
PFAM: PF00177.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0893330	RZC65734.1	78.704	108	10	2	1	107	1	96	2.57e-47	158

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0893330	sp\|Q9GFM6\|RR7_CALFL	57.895	114	40	3	1	107	1	113	2.11e-36	124

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0893330	A0A4Y7JXC4	78.704	108	10	2	1	107	1	96	1.23e-47	158

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0366560	Msa0893330	0.835979	1.224109e-56	-8.615850e-47
Msa0459360	Msa0893330	0.814809	1.275022e-51	-8.615850e-47
Msa0506110	Msa0893330	0.862878	3.709333e-64	-8.615850e-47
Msa0590170	Msa0893330	0.810692	1.015671e-50	-8.615850e-47
Msa0893330	Msa0983970	0.987601	7.532767e-171	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0893330	MtrunA17_CPg0492421	53.913	115	45	3	1	107	1	115	4.84e-32	110
Msa0893330	MtrunA17_Chr4g0016101	53.913	115	45	3	1	107	1	115	4.84e-32	110
Msa0893330	MtrunA17_Chr3g0090841	53.913	115	45	3	1	107	1	115	4.84e-32	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0893330	ATCG00900.1	57.018	114	41	3	1	107	1	113	4.60e-35	118
Msa0893330	ATCG01240.1	57.018	114	41	3	1	107	1	113	4.60e-35	118

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTCCATTCGTTGGTTATT+AGG	0.220723	6_1:-76892805	Msa0893330:CDS
ACTAATCGATTACGATAAAT+TGG	0.238141	6_1:+76893047	None:intergenic
CATCAAGTTCCCGTTGAAAT+AGG	0.307921	6_1:-76892847	Msa0893330:CDS
GCTTGACATAAAACAGATAG+TGG	0.428818	6_1:+76892920	None:intergenic
AGGCGCATCCCGAAAACGTC+CGG	0.430208	6_1:-76892785	Msa0893330:CDS
GAAAAGAAATTTCCATTCGT+TGG	0.469094	6_1:-76892813	Msa0893330:CDS
TGTGTGGATCCTATTTCAAC+GGG	0.470768	6_1:+76892838	None:intergenic
TTATCTATCGAGCTATGAAA+AGG	0.512086	6_1:-76892967	Msa0893330:CDS
TAATCGATTAGTTAACATGT+TGG	0.538486	6_1:-76893036	Msa0893330:CDS
TTGTGTGGATCCTATTTCAA+CGG	0.548545	6_1:+76892837	None:intergenic
GGCGCATCCCGAAAACGTCC+GGG	0.561163	6_1:-76892784	Msa0893330:CDS
TGCGCCTAATAACCAACGAA+TGG	0.589736	6_1:+76892801	None:intergenic
TTAAAAGCCATATTTCAACC+CGG	0.649181	6_1:+76892766	None:intergenic
GTTAACCGTATTATGAAACA+CGG	0.650772	6_1:-76893014	Msa0893330:CDS
TTTACACTCATGTCACGGCG+AGG	0.671159	6_1:-76893098	None:intergenic
TTTATGTCAAGCAATATGTG+GGG	0.713803	6_1:-76892909	Msa0893330:CDS
GTTGAAATAGGATCCACACA+AGG	0.731975	6_1:-76892835	Msa0893330:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAATTGGATCAGATTTTG+CGG	+	chr6_1:76892811-76892830	None:intergenic	25.0%
!	ACTAATCGATTACGATAAAT+TGG	+	chr6_1:76892825-76892844	None:intergenic	25.0%
!!	TAATCGATTAGTTAACATGT+TGG	-	chr6_1:76892833-76892852	Msa0893330:CDS	25.0%
!!	TTTTTCCGTGTTTCATAATA+CGG	+	chr6_1:76892863-76892882	None:intergenic	25.0%
!	AAACACGGAAAAAAAATCAT+TGG	-	chr6_1:76892870-76892889	Msa0893330:intron	25.0%
!!	TTTTATGTCAAGCAATATGT+GGG	-	chr6_1:76892959-76892978	Msa0893330:CDS	25.0%
	GTTAACCGTATTATGAAACA+CGG	-	chr6_1:76892855-76892874	Msa0893330:CDS	30.0%
	TTATCTATCGAGCTATGAAA+AGG	-	chr6_1:76892902-76892921	Msa0893330:CDS	30.0%
!	GTTTTATGTCAAGCAATATG+TGG	-	chr6_1:76892958-76892977	Msa0893330:CDS	30.0%
	TTTATGTCAAGCAATATGTG+GGG	-	chr6_1:76892960-76892979	Msa0893330:CDS	30.0%
	GAAAAGAAATTTCCATTCGT+TGG	-	chr6_1:76893056-76893075	Msa0893330:CDS	30.0%
!	ATTTCCATTCGTTGGTTATT+AGG	-	chr6_1:76893064-76893083	Msa0893330:CDS	30.0%
	GCTTGACATAAAACAGATAG+TGG	+	chr6_1:76892952-76892971	None:intergenic	35.0%
	TTGTGTGGATCCTATTTCAA+CGG	+	chr6_1:76893035-76893054	None:intergenic	35.0%
!	GGAAATTTCTTTTCCTTGTG+TGG	+	chr6_1:76893050-76893069	None:intergenic	35.0%
	CATCAAGTTCCCGTTGAAAT+AGG	-	chr6_1:76893022-76893041	Msa0893330:CDS	40.0%
	TGTGTGGATCCTATTTCAAC+GGG	+	chr6_1:76893034-76893053	None:intergenic	40.0%
	GTTGAAATAGGATCCACACA+AGG	-	chr6_1:76893034-76893053	Msa0893330:CDS	40.0%
	TGCGCCTAATAACCAACGAA+TGG	+	chr6_1:76893071-76893090	None:intergenic	45.0%
	AGGCGCATCCCGAAAACGTC+CGG	-	chr6_1:76893084-76893103	Msa0893330:CDS	60.0%
	GGCGCATCCCGAAAACGTCC+GGG	-	chr6_1:76893085-76893104	Msa0893330:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	76892780	76893111	76892780	ID=Msa0893330;Name=Msa0893330
chr6_1	mRNA	76892780	76893111	76892780	ID=Msa0893330-mRNA-1;Parent=Msa0893330;Name=Msa0893330-mRNA-1;_AED=0.49;_eAED=0.75;_QI=0\|0\|0\|1\|0\|0\|2\|0\|107
chr6_1	exon	76892885	76893111	76892885	ID=Msa0893330-mRNA-1:exon:12841;Parent=Msa0893330-mRNA-1
chr6_1	exon	76892780	76892876	76892780	ID=Msa0893330-mRNA-1:exon:12840;Parent=Msa0893330-mRNA-1
chr6_1	CDS	76892885	76893111	76892885	ID=Msa0893330-mRNA-1:cds;Parent=Msa0893330-mRNA-1
chr6_1	CDS	76892780	76892876	76892780	ID=Msa0893330-mRNA-1:cds;Parent=Msa0893330-mRNA-1

Gene Sequence	>Msa0893330 ATGTCACGGCGAGGTACTGCAGAAAAAACCGCAAAATCTGATCCAATTTATCGTAATCGATTAGTTAACATGTTGGTTAACCGTATTATGAAACACGGAAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCTGTTTTATGTCAAGCAATATGTGGGGTAACTCTCGATATAGCAGTAACGCATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGAAATTTCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCGGGTTGA
Protein sequence	>Msa0893330 MSRRGTAEKTAKSDPIYRNRLVNMLVNRIMKHGKKIIGLSNYLSSYEKDSTKDRNKSTICFMSSNMWGNSRYSSNASTHQVPVEIGSTQGKEISIRWLLGASRKRPG*