AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0909130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0909130 MtrunA17_Chr8g0355661 94.872 156 8 0 1 156 1 156 1.42e-108 305
Msa0909130 MtrunA17_Chr8g0355741 79.618 157 31 1 1 156 1 157 3.47e-89 256
Msa0909130 MtrunA17_Chr8g0355721 77.070 157 34 2 1 156 1 156 3.70e-87 251
Msa0909130 MtrunA17_Chr8g0355811 73.885 157 39 2 1 156 1 156 2.00e-83 242
Msa0909130 MtrunA17_Chr8g0355791 73.885 157 39 2 1 156 1 156 1.91e-80 234
Msa0909130 MtrunA17_Chr8g0355781 73.026 152 40 1 1 151 1 152 2.20e-80 234
Msa0909130 MtrunA17_Chr8g0355801 71.975 157 42 2 1 156 1 156 3.57e-79 231
Msa0909130 MtrunA17_Chr8g0355641 69.872 156 46 1 1 155 1 156 4.26e-79 231
Msa0909130 MtrunA17_Chr8g0355821 70.323 155 44 2 1 154 1 154 8.59e-79 230
Msa0909130 MtrunA17_Chr8g0355771 71.975 157 42 2 1 156 1 156 1.45e-78 229
Msa0909130 MtrunA17_Chr8g0355761 71.711 152 42 1 1 151 1 152 2.56e-78 229
Msa0909130 MtrunA17_Chr1g0160371 41.176 153 86 3 1 150 1 152 6.57e-38 126
Msa0909130 MtrunA17_Chr1g0160381 38.562 153 90 3 1 150 5 156 7.49e-37 124
Msa0909130 MtrunA17_Chr1g0160391 40.127 157 83 6 1 148 1 155 1.64e-29 105
Msa0909130 MtrunA17_Chr1g0160801 38.776 147 82 4 7 151 7 147 3.58e-28 101
Msa0909130 MtrunA17_Chr8g0339261 35.862 145 89 4 6 148 8 150 3.37e-26 97.1
Msa0909130 MtrunA17_Chr1g0160351 40.860 93 52 2 61 150 1 93 9.66e-20 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0909130 AT1G70840.1 38.000 150 89 4 3 149 20 168 2.40e-26 98.2
Msa0909130 AT5G28010.1 36.667 150 91 4 3 149 15 163 2.54e-26 98.2
Msa0909130 AT5G28010.2 36.667 150 91 4 3 149 15 163 2.54e-26 98.2
Msa0909130 AT1G70830.5 38.667 150 88 4 3 149 22 170 6.25e-26 97.1
Msa0909130 AT1G70830.3 38.667 150 88 4 3 149 22 170 1.22e-25 97.1
Msa0909130 AT1G70890.1 37.333 150 90 4 3 149 7 155 2.72e-25 95.1
Msa0909130 AT1G70830.2 38.667 150 88 4 3 149 22 170 5.35e-25 96.7
Msa0909130 AT1G70830.1 38.667 150 88 4 3 149 22 170 1.60e-24 97.1
Msa0909130 AT1G70830.1 38.000 150 89 4 3 149 184 332 1.28e-23 94.7
Msa0909130 AT1G70830.4 38.000 150 89 4 3 149 137 285 3.13e-24 95.5
Msa0909130 AT1G70830.4 35.484 93 58 2 59 149 31 123 8.87e-12 61.6
Msa0909130 AT5G28000.1 35.333 150 93 4 3 149 13 161 4.94e-23 89.4
Msa0909130 AT1G23120.1 28.859 149 102 2 1 149 1 145 9.00e-23 88.2
Msa0909130 AT1G70850.2 36.667 150 91 4 3 149 9 157 1.27e-22 90.1
Msa0909130 AT1G23120.2 29.371 143 97 2 7 149 57 195 2.19e-22 88.6
Msa0909130 AT1G70850.3 36.667 150 91 4 3 149 9 157 3.88e-22 90.5
Msa0909130 AT1G70850.3 36.429 140 85 4 13 149 175 313 2.05e-20 85.9
Msa0909130 AT1G70850.1 36.667 150 91 4 3 149 9 157 3.88e-22 90.5
Msa0909130 AT1G70850.1 36.429 140 85 4 13 149 175 313 2.05e-20 85.9
Msa0909130 AT1G70870.1 38.542 96 59 0 55 150 43 138 3.17e-21 84.0
Msa0909130 AT1G14930.1 30.769 156 106 2 1 155 1 155 3.20e-21 84.7
Msa0909130 AT4G23670.1 32.895 152 100 2 1 151 1 151 4.97e-21 84.0
Msa0909130 AT1G23130.1 31.544 149 99 3 3 149 10 157 9.80e-21 83.6
Msa0909130 AT2G01520.1 30.263 152 104 2 1 151 1 151 6.05e-20 81.3
Msa0909130 AT4G14060.1 29.605 152 105 2 1 151 1 151 1.37e-18 77.8
Msa0909130 AT2G01530.1 29.605 152 105 2 1 151 1 151 3.50e-18 76.6
Msa0909130 AT1G70880.1 30.667 150 100 4 3 149 8 156 3.58e-18 76.6
Msa0909130 AT1G14940.1 28.846 156 109 2 1 155 1 155 4.13e-18 76.6
Msa0909130 AT4G23680.1 29.139 151 105 2 1 150 1 150 1.37e-17 75.1
Msa0909130 AT1G30990.1 30.000 150 102 3 1 149 1 148 1.68e-17 74.7
Msa0909130 AT1G14950.1 28.846 156 109 2 1 155 1 155 5.45e-17 73.6
Msa0909130 AT1G35260.1 30.137 146 97 4 7 149 6 149 9.98e-17 72.8
Msa0909130 AT1G14960.1 28.477 151 106 2 1 150 1 150 5.71e-15 68.2
Msa0909130 AT3G26450.1 28.105 153 107 3 1 151 1 152 6.09e-15 68.2
Msa0909130 AT3G26460.1 27.451 153 108 3 1 151 1 152 6.65e-14 65.5
Msa0909130 AT1G35310.1 27.338 139 97 4 13 149 12 148 1.47e-13 64.7
Msa0909130 AT1G24020.1 30.263 152 103 3 1 150 1 151 2.87e-12 61.2
Msa0909130 AT1G24020.2 30.263 152 103 3 1 150 1 151 2.87e-12 61.2
Msa0909130 AT1G14940.2 25.641 156 98 2 1 155 1 139 6.05e-11 57.4

Find 30 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCACCTTCATGAAGTT+TGG 0.299460 6_2:+10315454 None:intergenic
AAGTTCAGTACTAAGTTTAC+CGG 0.399540 6_2:+10315565 None:intergenic
AACATTAGCGAAAGAGTTCA+TGG 0.404572 6_2:-10315479 Msa0909130:CDS
AACAAGTCCATCCAGCCATT+TGG 0.409540 6_2:+10314903 None:intergenic
ATCATTGAAGTAATTGATAA+TGG 0.438898 6_2:-10314990 Msa0909130:CDS
ATGGCAGTGGAGCTGCAAAA+TGG 0.452179 6_2:-10314965 Msa0909130:CDS
AAACTCACTTTCAAGCTCTT+CGG 0.455962 6_2:-10315050 Msa0909130:CDS
AAACACTGGACTTATGTCAT+AGG 0.470664 6_2:-10315410 Msa0909130:intron
AACTTCATGAAGGTGAAGAT+TGG 0.494334 6_2:-10315451 Msa0909130:CDS
AGACATTGATGAGCACTATA+AGG 0.495099 6_2:-10315024 Msa0909130:CDS
AGATTGGCATCACACTGATT+CGG 0.519723 6_2:-10315435 Msa0909130:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 6_2:-10314914 Msa0909130:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 6_2:-10314918 Msa0909130:CDS
GGTAAACTTAGTACTGAACT+TGG 0.549891 6_2:-10315563 Msa0909130:CDS
AAGTCCATCCAGCCATTTGG+AGG 0.551025 6_2:+10314906 None:intergenic
CTCACTTTCAAGCTCTTCGG+TGG 0.553344 6_2:-10315047 Msa0909130:CDS
TGACATCACAACAGATGGTA+AGG 0.554203 6_2:-10315117 Msa0909130:intron
CATTCAGAAATGGTGCTCGC+CGG 0.555555 6_2:-10315584 None:intergenic
ATTGATAATGGTGATGGCAG+TGG 0.556824 6_2:-10314978 Msa0909130:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 6_2:-10314910 Msa0909130:CDS
ATTGATCTTCTCATATTCAA+CGG 0.576809 6_2:+10314941 None:intergenic
GTTGATGCTCATCTTGTCAA+AGG 0.600604 6_2:-10314861 Msa0909130:CDS
ACACTGATTCGGTTAAACAC+TGG 0.614381 6_2:-10315424 Msa0909130:CDS
GAAGTAATTGATAATGGTGA+TGG 0.618480 6_2:-10314984 Msa0909130:CDS
TAGTTTGAAGAACTTGTCGA+CGG 0.618944 6_2:+10315529 None:intergenic
GTAAACTTAGTACTGAACTT+GGG 0.625486 6_2:-10315562 Msa0909130:CDS
AGTTTGAAGAACTTGTCGAC+GGG 0.627390 6_2:+10315530 None:intergenic
CATGGTGCCAAACTTCATGA+AGG 0.632424 6_2:-10315461 Msa0909130:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.644461 6_2:+10314907 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 6_2:-10314937 Msa0909130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATAAACAAGATGATA+TGG + chr6_2:10315065-10315084 None:intergenic 15.0%
!! ATAGAGTTATGAAAAATATA+TGG + chr6_2:10315244-10315263 None:intergenic 15.0%
!!! ATACACTGTTTTAATTTAAT+TGG - chr6_2:10315264-10315283 Msa0909130:intron 15.0%
!! TATAAATAAACAAGATGATA+TGG + chr6_2:10315065-10315084 None:intergenic 15.0%
!! ATAGAGTTATGAAAAATATA+TGG + chr6_2:10315244-10315263 None:intergenic 15.0%
!!! ATACACTGTTTTAATTTAAT+TGG - chr6_2:10315264-10315283 Msa0909130:intron 15.0%
!! TGCTCTTATTAATTGAATTA+AGG + chr6_2:10315095-10315114 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG - chr6_2:10315096-10315115 Msa0909130:CDS 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG - chr6_2:10315097-10315116 Msa0909130:CDS 20.0%
!! CATAATCTTCTTATAAAGTA+TGG + chr6_2:10315124-10315143 None:intergenic 20.0%
!! ACTTTATCTTTATCATCTTT+AGG + chr6_2:10315148-10315167 None:intergenic 20.0%
!! ATCATTGAAGTAATTGATAA+TGG - chr6_2:10315427-10315446 Msa0909130:CDS 20.0%
!! TGCTCTTATTAATTGAATTA+AGG + chr6_2:10315095-10315114 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG - chr6_2:10315096-10315115 Msa0909130:CDS 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG - chr6_2:10315097-10315116 Msa0909130:CDS 20.0%
!! CATAATCTTCTTATAAAGTA+TGG + chr6_2:10315124-10315143 None:intergenic 20.0%
!! ACTTTATCTTTATCATCTTT+AGG + chr6_2:10315148-10315167 None:intergenic 20.0%
!! ATCATTGAAGTAATTGATAA+TGG - chr6_2:10315427-10315446 Msa0909130:CDS 20.0%
! AATAAACAAGATGATATGGA+GGG + chr6_2:10315061-10315080 None:intergenic 25.0%
! AAATAAACAAGATGATATGG+AGG + chr6_2:10315062-10315081 None:intergenic 25.0%
! ATTGATCTTCTCATATTCAA+CGG + chr6_2:10315479-10315498 None:intergenic 25.0%
! AATAAACAAGATGATATGGA+GGG + chr6_2:10315061-10315080 None:intergenic 25.0%
! AAATAAACAAGATGATATGG+AGG + chr6_2:10315062-10315081 None:intergenic 25.0%
! ATTGATCTTCTCATATTCAA+CGG + chr6_2:10315479-10315498 None:intergenic 25.0%
AAGTTCAGTACTAAGTTTAC+CGG + chr6_2:10314855-10314874 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG - chr6_2:10314855-10314874 Msa0909130:CDS 30.0%
!! GAAGTAATTGATAATGGTGA+TGG - chr6_2:10315433-10315452 Msa0909130:CDS 30.0%
TGAATATGAGAAGATCAATG+AGG - chr6_2:10315480-10315499 Msa0909130:CDS 30.0%
AAGTTCAGTACTAAGTTTAC+CGG + chr6_2:10314855-10314874 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG - chr6_2:10314855-10314874 Msa0909130:CDS 30.0%
!! GAAGTAATTGATAATGGTGA+TGG - chr6_2:10315433-10315452 Msa0909130:CDS 30.0%
TGAATATGAGAAGATCAATG+AGG - chr6_2:10315480-10315499 Msa0909130:CDS 30.0%
! GGTAAACTTAGTACTGAACT+TGG - chr6_2:10314854-10314873 Msa0909130:CDS 35.0%
TAGTTTGAAGAACTTGTCGA+CGG + chr6_2:10314891-10314910 None:intergenic 35.0%
AACATTAGCGAAAGAGTTCA+TGG - chr6_2:10314938-10314957 Msa0909130:CDS 35.0%
ATCTTCACCTTCATGAAGTT+TGG + chr6_2:10314966-10314985 None:intergenic 35.0%
AACTTCATGAAGGTGAAGAT+TGG - chr6_2:10314966-10314985 Msa0909130:CDS 35.0%
AAACACTGGACTTATGTCAT+AGG - chr6_2:10315007-10315026 Msa0909130:CDS 35.0%
AAACTCACTTTCAAGCTCTT+CGG - chr6_2:10315367-10315386 Msa0909130:intron 35.0%
AGACATTGATGAGCACTATA+AGG - chr6_2:10315393-10315412 Msa0909130:intron 35.0%
! GGTAAACTTAGTACTGAACT+TGG - chr6_2:10314854-10314873 Msa0909130:CDS 35.0%
TAGTTTGAAGAACTTGTCGA+CGG + chr6_2:10314891-10314910 None:intergenic 35.0%
AACATTAGCGAAAGAGTTCA+TGG - chr6_2:10314938-10314957 Msa0909130:CDS 35.0%
ATCTTCACCTTCATGAAGTT+TGG + chr6_2:10314966-10314985 None:intergenic 35.0%
AACTTCATGAAGGTGAAGAT+TGG - chr6_2:10314966-10314985 Msa0909130:CDS 35.0%
AAACACTGGACTTATGTCAT+AGG - chr6_2:10315007-10315026 Msa0909130:CDS 35.0%
AAACTCACTTTCAAGCTCTT+CGG - chr6_2:10315367-10315386 Msa0909130:intron 35.0%
AGACATTGATGAGCACTATA+AGG - chr6_2:10315393-10315412 Msa0909130:intron 35.0%
AGTTTGAAGAACTTGTCGAC+GGG + chr6_2:10314890-10314909 None:intergenic 40.0%
! AGATTGGCATCACACTGATT+CGG - chr6_2:10314982-10315001 Msa0909130:CDS 40.0%
!! ACACTGATTCGGTTAAACAC+TGG - chr6_2:10314993-10315012 Msa0909130:CDS 40.0%
ACCATCTGTTGTGATGTCAA+GGG + chr6_2:10315299-10315318 None:intergenic 40.0%
TACCATCTGTTGTGATGTCA+AGG + chr6_2:10315300-10315319 None:intergenic 40.0%
TGACATCACAACAGATGGTA+AGG - chr6_2:10315300-10315319 Msa0909130:intron 40.0%
!! ATTGATAATGGTGATGGCAG+TGG - chr6_2:10315439-10315458 Msa0909130:CDS 40.0%
GTTGATGCTCATCTTGTCAA+AGG - chr6_2:10315556-10315575 Msa0909130:CDS 40.0%
AGTTTGAAGAACTTGTCGAC+GGG + chr6_2:10314890-10314909 None:intergenic 40.0%
! AGATTGGCATCACACTGATT+CGG - chr6_2:10314982-10315001 Msa0909130:CDS 40.0%
!! ACACTGATTCGGTTAAACAC+TGG - chr6_2:10314993-10315012 Msa0909130:CDS 40.0%
ACCATCTGTTGTGATGTCAA+GGG + chr6_2:10315299-10315318 None:intergenic 40.0%
TACCATCTGTTGTGATGTCA+AGG + chr6_2:10315300-10315319 None:intergenic 40.0%
TGACATCACAACAGATGGTA+AGG - chr6_2:10315300-10315319 Msa0909130:intron 40.0%
!! ATTGATAATGGTGATGGCAG+TGG - chr6_2:10315439-10315458 Msa0909130:CDS 40.0%
GTTGATGCTCATCTTGTCAA+AGG - chr6_2:10315556-10315575 Msa0909130:CDS 40.0%
! CATGGTGCCAAACTTCATGA+AGG - chr6_2:10314956-10314975 Msa0909130:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG - chr6_2:10315499-10315518 Msa0909130:CDS 45.0%
ATATTGACCCTCCAAATGGC+TGG - chr6_2:10315503-10315522 Msa0909130:CDS 45.0%
AACAAGTCCATCCAGCCATT+TGG + chr6_2:10315517-10315536 None:intergenic 45.0%
! CATGGTGCCAAACTTCATGA+AGG - chr6_2:10314956-10314975 Msa0909130:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG - chr6_2:10315499-10315518 Msa0909130:CDS 45.0%
ATATTGACCCTCCAAATGGC+TGG - chr6_2:10315503-10315522 Msa0909130:CDS 45.0%
AACAAGTCCATCCAGCCATT+TGG + chr6_2:10315517-10315536 None:intergenic 45.0%
GCCCTTGACATCACAACAGA+TGG - chr6_2:10315295-10315314 Msa0909130:intron 50.0%
CTCACTTTCAAGCTCTTCGG+TGG - chr6_2:10315370-10315389 Msa0909130:intron 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG - chr6_2:10315452-10315471 Msa0909130:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG + chr6_2:10315513-10315532 None:intergenic 50.0%
AAGTCCATCCAGCCATTTGG+AGG + chr6_2:10315514-10315533 None:intergenic 50.0%
GCCCTTGACATCACAACAGA+TGG - chr6_2:10315295-10315314 Msa0909130:intron 50.0%
CTCACTTTCAAGCTCTTCGG+TGG - chr6_2:10315370-10315389 Msa0909130:intron 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG - chr6_2:10315452-10315471 Msa0909130:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG + chr6_2:10315513-10315532 None:intergenic 50.0%
AAGTCCATCCAGCCATTTGG+AGG + chr6_2:10315514-10315533 None:intergenic 50.0%
TGACCCTCCAAATGGCTGGA+TGG - chr6_2:10315507-10315526 Msa0909130:CDS 55.0%
TGACCCTCCAAATGGCTGGA+TGG - chr6_2:10315507-10315526 Msa0909130:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 10314842 10315597 10314842 ID=Msa0909130;Name=Msa0909130
chr6_2 mRNA 10314842 10315597 10314842 ID=Msa0909130-mRNA-1;Parent=Msa0909130;Name=Msa0909130-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|156
chr6_2 exon 10315411 10315597 10315411 ID=Msa0909130-mRNA-1:exon:2654;Parent=Msa0909130-mRNA-1
chr6_2 exon 10314842 10315125 10314842 ID=Msa0909130-mRNA-1:exon:2653;Parent=Msa0909130-mRNA-1
chr6_2 CDS 10315411 10315597 10315411 ID=Msa0909130-mRNA-1:cds;Parent=Msa0909130-mRNA-1
chr6_2 CDS 10314842 10315125 10314842 ID=Msa0909130-mRNA-1:cds;Parent=Msa0909130-mRNA-1
Gene Sequence

>Msa0909130

ATGGTGCTCGCCGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGTCGACAAGTTCTTCAAACTATTTGCATCAGAACTTCACGAAGTGCAAAACATTAGCGAAAGAGTTCATGGTGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTATGTCATAGATGGTAAGGTACACACATGTCATGAGAGTATCGAAGTTGATGAACAGAACAAAAAACTCACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAATGTGATCATTGAAGTAATTGATAATGGTGATGGCAGTGGAGCTGCAAAATGGACCGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTTGTTAGACAAAGTCACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCGCAAACATAGCTCTATAA

Protein sequence

>Msa0909130

MVLAGKLSTELGIKTPVDKFFKLFASELHEVQNISERVHGAKLHEGEDWHHTDSVKHWTYVIDGKVHTCHESIEVDEQNKKLTFKLFGGDIDEHYKVFNVIIEVIDNGDGSGAAKWTVEYEKINEDIDPPNGWMDLLDKVTREVDAHLVKGANIAL*