AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1242200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1242200 MtrunA17_Chr8g0355661 94.839 155 8 0 1 155 2 156 1.89e-107 303
Msa1242200 MtrunA17_Chr8g0355741 78.846 156 32 1 1 155 2 157 2.07e-87 252
Msa1242200 MtrunA17_Chr8g0355721 76.923 156 34 2 1 155 2 156 4.07e-86 249
Msa1242200 MtrunA17_Chr8g0355811 73.077 156 40 2 1 155 2 156 2.02e-81 237
Msa1242200 MtrunA17_Chr8g0355781 72.185 151 41 1 1 150 2 152 1.16e-78 229
Msa1242200 MtrunA17_Chr8g0355791 73.077 156 40 2 1 155 2 156 3.56e-78 229
Msa1242200 MtrunA17_Chr8g0355641 69.032 155 47 1 1 154 2 156 4.43e-77 226
Msa1242200 MtrunA17_Chr8g0355801 71.154 156 43 2 1 155 2 156 4.52e-77 226
Msa1242200 MtrunA17_Chr8g0355761 70.861 151 43 1 1 150 2 152 7.32e-77 225
Msa1242200 MtrunA17_Chr8g0355821 69.481 154 45 2 1 153 2 154 9.96e-77 225
Msa1242200 MtrunA17_Chr8g0355771 71.154 156 43 2 1 155 2 156 1.76e-76 224
Msa1242200 MtrunA17_Chr1g0160371 40.132 152 87 3 1 149 2 152 1.14e-35 120
Msa1242200 MtrunA17_Chr1g0160381 37.500 152 91 3 1 149 6 156 1.30e-34 118
Msa1242200 MtrunA17_Chr1g0160391 40.000 155 82 6 2 147 3 155 9.83e-29 103
Msa1242200 MtrunA17_Chr1g0160801 38.095 147 83 4 6 150 7 147 2.11e-26 97.1
Msa1242200 MtrunA17_Chr8g0339261 35.862 145 89 4 5 147 8 150 3.22e-26 97.1
Msa1242200 MtrunA17_Chr1g0160351 40.860 93 52 2 60 149 1 93 3.22e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1242200 AT5G28010.1 36.667 150 91 4 2 148 15 163 3.40e-26 97.4
Msa1242200 AT5G28010.2 36.667 150 91 4 2 148 15 163 3.40e-26 97.4
Msa1242200 AT1G70840.1 38.000 150 89 4 2 148 20 168 4.35e-26 97.4
Msa1242200 AT1G70830.5 38.667 150 88 4 2 148 22 170 1.22e-25 96.3
Msa1242200 AT1G70830.3 38.667 150 88 4 2 148 22 170 2.55e-25 96.3
Msa1242200 AT1G70830.2 38.667 150 88 4 2 148 22 170 1.06e-24 95.9
Msa1242200 AT1G70830.1 38.667 150 88 4 2 148 22 170 3.15e-24 96.3
Msa1242200 AT1G70830.1 38.000 150 89 4 2 148 184 332 2.20e-23 94.0
Msa1242200 AT1G70890.1 36.667 150 91 4 2 148 7 155 6.27e-24 91.7
Msa1242200 AT1G70830.4 38.000 150 89 4 2 148 137 285 6.30e-24 94.7
Msa1242200 AT1G70830.4 35.484 93 58 2 58 148 31 123 9.58e-12 61.6
Msa1242200 AT5G28000.1 35.333 150 93 4 2 148 13 161 7.94e-23 89.0
Msa1242200 AT1G70850.2 36.667 150 91 4 2 148 9 157 2.24e-22 89.4
Msa1242200 AT1G23120.1 29.371 143 97 2 6 148 7 145 3.50e-22 86.7
Msa1242200 AT1G70850.3 36.667 150 91 4 2 148 9 157 6.50e-22 89.7
Msa1242200 AT1G70850.3 37.063 143 80 5 12 148 175 313 2.73e-20 85.5
Msa1242200 AT1G70850.1 36.667 150 91 4 2 148 9 157 6.50e-22 89.7
Msa1242200 AT1G70850.1 37.063 143 80 5 12 148 175 313 2.73e-20 85.5
Msa1242200 AT1G23120.2 29.371 143 97 2 6 148 57 195 9.34e-22 87.0
Msa1242200 AT1G70870.1 38.542 96 59 0 54 149 43 138 2.79e-21 84.3
Msa1242200 AT1G23130.1 31.544 149 99 3 2 148 10 157 1.36e-20 83.2
Msa1242200 AT1G14930.1 30.323 155 106 2 1 154 2 155 4.50e-20 81.6
Msa1242200 AT4G23670.1 32.886 149 98 2 3 150 4 151 4.60e-20 81.3
Msa1242200 AT2G01520.1 30.201 149 102 2 3 150 4 151 6.77e-19 78.6
Msa1242200 AT1G70880.1 30.667 150 100 4 2 148 8 156 6.61e-18 75.9
Msa1242200 AT4G14060.1 29.530 149 103 2 3 150 4 151 1.08e-17 75.1
Msa1242200 AT2G01530.1 29.530 149 103 2 3 150 4 151 3.57e-17 73.9
Msa1242200 AT1G14940.1 28.387 155 109 2 1 154 2 155 5.49e-17 73.6
Msa1242200 AT1G35260.1 30.137 146 97 4 6 148 6 149 9.84e-17 72.8
Msa1242200 AT4G23680.1 29.054 148 103 2 3 149 4 150 1.71e-16 72.0
Msa1242200 AT1G14950.1 28.758 153 107 2 3 154 4 155 4.97e-16 70.9
Msa1242200 AT1G30990.1 29.530 149 102 3 1 148 2 148 6.33e-16 70.9
Msa1242200 AT3G26450.1 28.000 150 105 3 3 150 4 152 5.44e-14 65.5
Msa1242200 AT1G14960.1 28.188 149 105 2 2 149 3 150 1.28e-13 64.7
Msa1242200 AT1G35310.1 27.338 139 97 4 12 148 12 148 3.11e-13 63.5
Msa1242200 AT3G26460.1 27.333 150 106 3 3 150 4 152 5.21e-13 63.2
Msa1242200 AT1G24020.1 29.814 161 88 5 2 149 3 151 1.16e-11 59.7
Msa1242200 AT1G24020.2 29.814 161 88 5 2 149 3 151 1.16e-11 59.7

Find 32 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCACCTTCATGAAGTT+TGG 0.299460 8_2:-26307798 None:intergenic
AACATTAGCGAAAGAGTTCC+TGG 0.390706 8_2:+26307773 Msa1242200:CDS
AAGTTCAGTACTAAGTTTAC+CGG 0.399540 8_2:-26307687 None:intergenic
AACAAGTCCATCCAGCCATT+TGG 0.409540 8_2:-26308348 None:intergenic
ATCATTGAAGTAATTGATAA+TGG 0.438898 8_2:+26308261 Msa1242200:CDS
ATGGCAGTGGAGCTGCAAAA+TGG 0.452179 8_2:+26308286 Msa1242200:CDS
AAACTCACTTTCAAGCTCTT+CGG 0.455962 8_2:+26308201 Msa1242200:CDS
AATGGCTGGATGGACTTGTT+AGG 0.459576 8_2:+26308351 Msa1242200:CDS
AAACACTGGACTTATGTCAT+AGG 0.470664 8_2:+26307842 Msa1242200:CDS
CCTTCATGAAGTTTGGCACC+AGG 0.490652 8_2:-26307791 None:intergenic
AACTTCATGAAGGTGAAGAT+TGG 0.494334 8_2:+26307801 Msa1242200:CDS
AGACATTGATGAGCACTATA+AGG 0.495099 8_2:+26308227 Msa1242200:CDS
AGATTGGCATCACACTGATT+CGG 0.519723 8_2:+26307817 Msa1242200:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8_2:+26308337 Msa1242200:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8_2:+26308333 Msa1242200:CDS
GGTAAACTTAGTACTGAACT+TGG 0.549891 8_2:+26307689 Msa1242200:CDS
AAGTCCATCCAGCCATTTGG+AGG 0.551025 8_2:-26308345 None:intergenic
CTCACTTTCAAGCTCTTCGG+TGG 0.553344 8_2:+26308204 Msa1242200:CDS
TGACATCACAACAGATGGTA+AGG 0.554203 8_2:+26308134 Msa1242200:intron
ATTGATAATGGTGATGGCAG+TGG 0.556824 8_2:+26308273 Msa1242200:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8_2:+26308341 Msa1242200:CDS
ATTGATCTTCTCATATTCAA+CGG 0.576809 8_2:-26308310 None:intergenic
AAATGGTGTAGTGTGCTCGC+CGG 0.594337 8_2:+26307668 None:intergenic
GTTGATGCTCATCTTGTCAA+AGG 0.600604 8_2:+26308390 Msa1242200:CDS
ACACTGATTCGGTTAAACAC+TGG 0.614381 8_2:+26307828 Msa1242200:CDS
GAAGTAATTGATAATGGTGA+TGG 0.618480 8_2:+26308267 Msa1242200:CDS
TAGTTTGAAGAACTTGTCGA+CGG 0.618944 8_2:-26307723 None:intergenic
GTAAACTTAGTACTGAACTT+GGG 0.625486 8_2:+26307690 Msa1242200:CDS
AGTTTGAAGAACTTGTCGAC+GGG 0.627390 8_2:-26307722 None:intergenic
CCTGGTGCCAAACTTCATGA+AGG 0.632238 8_2:+26307791 Msa1242200:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.644461 8_2:-26308344 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 8_2:+26308314 Msa1242200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATAAACAAGATGATA+TGG - chr8_2:26307900-26307919 None:intergenic 15.0%
!! ATAGAGTAATGAAAAATATA+TGG - chr8_2:26308078-26308097 None:intergenic 15.0%
!!! ATACACTGTTTTAATTTAAT+TGG + chr8_2:26308098-26308117 Msa1242200:intron 15.0%
!! TATAAATAAACAAGATGATA+TGG - chr8_2:26307900-26307919 None:intergenic 15.0%
!! ATAGAGTAATGAAAAATATA+TGG - chr8_2:26308078-26308097 None:intergenic 15.0%
!!! ATACACTGTTTTAATTTAAT+TGG + chr8_2:26308098-26308117 Msa1242200:intron 15.0%
!! TGCTCTTATTAATTGAATTA+AGG - chr8_2:26307930-26307949 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG + chr8_2:26307931-26307950 Msa1242200:intron 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG + chr8_2:26307932-26307951 Msa1242200:intron 20.0%
!! CATAATCTTCATATAAAGTA+TGG - chr8_2:26307959-26307978 None:intergenic 20.0%
!! ACTTTATCTTTATCATCTTT+AGG - chr8_2:26307983-26308002 None:intergenic 20.0%
!! ATCATTGAAGTAATTGATAA+TGG + chr8_2:26308261-26308280 Msa1242200:CDS 20.0%
!! TGCTCTTATTAATTGAATTA+AGG - chr8_2:26307930-26307949 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG + chr8_2:26307931-26307950 Msa1242200:intron 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG + chr8_2:26307932-26307951 Msa1242200:intron 20.0%
!! CATAATCTTCATATAAAGTA+TGG - chr8_2:26307959-26307978 None:intergenic 20.0%
!! ACTTTATCTTTATCATCTTT+AGG - chr8_2:26307983-26308002 None:intergenic 20.0%
!! ATCATTGAAGTAATTGATAA+TGG + chr8_2:26308261-26308280 Msa1242200:CDS 20.0%
! AATAAACAAGATGATATGGA+GGG - chr8_2:26307896-26307915 None:intergenic 25.0%
! AAATAAACAAGATGATATGG+AGG - chr8_2:26307897-26307916 None:intergenic 25.0%
! ATTGATCTTCTCATATTCAA+CGG - chr8_2:26308313-26308332 None:intergenic 25.0%
! AATAAACAAGATGATATGGA+GGG - chr8_2:26307896-26307915 None:intergenic 25.0%
! AAATAAACAAGATGATATGG+AGG - chr8_2:26307897-26307916 None:intergenic 25.0%
! ATTGATCTTCTCATATTCAA+CGG - chr8_2:26308313-26308332 None:intergenic 25.0%
AAGTTCAGTACTAAGTTTAC+CGG - chr8_2:26307690-26307709 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG + chr8_2:26307690-26307709 Msa1242200:CDS 30.0%
!! GAAGTAATTGATAATGGTGA+TGG + chr8_2:26308267-26308286 Msa1242200:CDS 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8_2:26308314-26308333 Msa1242200:CDS 30.0%
AAGTTCAGTACTAAGTTTAC+CGG - chr8_2:26307690-26307709 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG + chr8_2:26307690-26307709 Msa1242200:CDS 30.0%
!! GAAGTAATTGATAATGGTGA+TGG + chr8_2:26308267-26308286 Msa1242200:CDS 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8_2:26308314-26308333 Msa1242200:CDS 30.0%
! GGTAAACTTAGTACTGAACT+TGG + chr8_2:26307689-26307708 Msa1242200:CDS 35.0%
TAGTTTGAAGAACTTGTCGA+CGG - chr8_2:26307726-26307745 None:intergenic 35.0%
ATCTTCACCTTCATGAAGTT+TGG - chr8_2:26307801-26307820 None:intergenic 35.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8_2:26307801-26307820 Msa1242200:CDS 35.0%
AAACACTGGACTTATGTCAT+AGG + chr8_2:26307842-26307861 Msa1242200:CDS 35.0%
AAACTCACTTTCAAGCTCTT+CGG + chr8_2:26308201-26308220 Msa1242200:CDS 35.0%
AGACATTGATGAGCACTATA+AGG + chr8_2:26308227-26308246 Msa1242200:CDS 35.0%
! GGTAAACTTAGTACTGAACT+TGG + chr8_2:26307689-26307708 Msa1242200:CDS 35.0%
TAGTTTGAAGAACTTGTCGA+CGG - chr8_2:26307726-26307745 None:intergenic 35.0%
ATCTTCACCTTCATGAAGTT+TGG - chr8_2:26307801-26307820 None:intergenic 35.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8_2:26307801-26307820 Msa1242200:CDS 35.0%
AAACACTGGACTTATGTCAT+AGG + chr8_2:26307842-26307861 Msa1242200:CDS 35.0%
AAACTCACTTTCAAGCTCTT+CGG + chr8_2:26308201-26308220 Msa1242200:CDS 35.0%
AGACATTGATGAGCACTATA+AGG + chr8_2:26308227-26308246 Msa1242200:CDS 35.0%
AGTTTGAAGAACTTGTCGAC+GGG - chr8_2:26307725-26307744 None:intergenic 40.0%
AACATTAGCGAAAGAGTTCC+TGG + chr8_2:26307773-26307792 Msa1242200:CDS 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8_2:26307817-26307836 Msa1242200:CDS 40.0%
!! ACACTGATTCGGTTAAACAC+TGG + chr8_2:26307828-26307847 Msa1242200:CDS 40.0%
ACCATCTGTTGTGATGTCAA+GGG - chr8_2:26308133-26308152 None:intergenic 40.0%
TACCATCTGTTGTGATGTCA+AGG - chr8_2:26308134-26308153 None:intergenic 40.0%
TGACATCACAACAGATGGTA+AGG + chr8_2:26308134-26308153 Msa1242200:intron 40.0%
!! ATTGATAATGGTGATGGCAG+TGG + chr8_2:26308273-26308292 Msa1242200:CDS 40.0%
GTTGATGCTCATCTTGTCAA+AGG + chr8_2:26308390-26308409 Msa1242200:CDS 40.0%
AGTTTGAAGAACTTGTCGAC+GGG - chr8_2:26307725-26307744 None:intergenic 40.0%
AACATTAGCGAAAGAGTTCC+TGG + chr8_2:26307773-26307792 Msa1242200:CDS 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8_2:26307817-26307836 Msa1242200:CDS 40.0%
!! ACACTGATTCGGTTAAACAC+TGG + chr8_2:26307828-26307847 Msa1242200:CDS 40.0%
ACCATCTGTTGTGATGTCAA+GGG - chr8_2:26308133-26308152 None:intergenic 40.0%
TACCATCTGTTGTGATGTCA+AGG - chr8_2:26308134-26308153 None:intergenic 40.0%
TGACATCACAACAGATGGTA+AGG + chr8_2:26308134-26308153 Msa1242200:intron 40.0%
!! ATTGATAATGGTGATGGCAG+TGG + chr8_2:26308273-26308292 Msa1242200:CDS 40.0%
GTTGATGCTCATCTTGTCAA+AGG + chr8_2:26308390-26308409 Msa1242200:CDS 40.0%
GAGGATATTGACCCTCCAAA+TGG + chr8_2:26308333-26308352 Msa1242200:CDS 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr8_2:26308337-26308356 Msa1242200:CDS 45.0%
AACAAGTCCATCCAGCCATT+TGG - chr8_2:26308351-26308370 None:intergenic 45.0%
! AATGGCTGGATGGACTTGTT+AGG + chr8_2:26308351-26308370 Msa1242200:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8_2:26308333-26308352 Msa1242200:CDS 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr8_2:26308337-26308356 Msa1242200:CDS 45.0%
AACAAGTCCATCCAGCCATT+TGG - chr8_2:26308351-26308370 None:intergenic 45.0%
! AATGGCTGGATGGACTTGTT+AGG + chr8_2:26308351-26308370 Msa1242200:CDS 45.0%
CCTTCATGAAGTTTGGCACC+AGG - chr8_2:26307794-26307813 None:intergenic 50.0%
! CCTGGTGCCAAACTTCATGA+AGG + chr8_2:26307791-26307810 Msa1242200:CDS 50.0%
GCCCTTGACATCACAACAGA+TGG + chr8_2:26308129-26308148 Msa1242200:intron 50.0%
CTCACTTTCAAGCTCTTCGG+TGG + chr8_2:26308204-26308223 Msa1242200:CDS 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG + chr8_2:26308286-26308305 Msa1242200:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8_2:26308347-26308366 None:intergenic 50.0%
AAGTCCATCCAGCCATTTGG+AGG - chr8_2:26308348-26308367 None:intergenic 50.0%
CCTTCATGAAGTTTGGCACC+AGG - chr8_2:26307794-26307813 None:intergenic 50.0%
! CCTGGTGCCAAACTTCATGA+AGG + chr8_2:26307791-26307810 Msa1242200:CDS 50.0%
GCCCTTGACATCACAACAGA+TGG + chr8_2:26308129-26308148 Msa1242200:intron 50.0%
CTCACTTTCAAGCTCTTCGG+TGG + chr8_2:26308204-26308223 Msa1242200:CDS 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG + chr8_2:26308286-26308305 Msa1242200:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8_2:26308347-26308366 None:intergenic 50.0%
AAGTCCATCCAGCCATTTGG+AGG - chr8_2:26308348-26308367 None:intergenic 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8_2:26308341-26308360 Msa1242200:CDS 55.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8_2:26308341-26308360 Msa1242200:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 26307680 26308428 26307680 ID=Msa1242200;Name=Msa1242200
chr8_2 mRNA 26307680 26308428 26307680 ID=Msa1242200-mRNA-1;Parent=Msa1242200;Name=Msa1242200-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|1|0.5|1|1|0.5|2|0|155
chr8_2 exon 26307680 26307863 26307680 ID=Msa1242200-mRNA-1:exon:9275;Parent=Msa1242200-mRNA-1
chr8_2 exon 26308148 26308428 26308148 ID=Msa1242200-mRNA-1:exon:9276;Parent=Msa1242200-mRNA-1
chr8_2 CDS 26307680 26307863 26307680 ID=Msa1242200-mRNA-1:cds;Parent=Msa1242200-mRNA-1
chr8_2 CDS 26308148 26308428 26308148 ID=Msa1242200-mRNA-1:cds;Parent=Msa1242200-mRNA-1
Gene Sequence

>Msa1242200

GTGCTCGCCGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGTCGACAAGTTCTTCAAACTATTTGCATCAGAACTTCACGAAGTGCAAAACATTAGCGAAAGAGTTCCTGGTGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTATGTCATAGATGGTAAGGTACACACATGTCATGAGAGTATCGAAGTTGATGAACAGAACAAAAAACTCACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAATGTGATCATTGAAGTAATTGATAATGGTGATGGCAGTGGAGCTGCAAAATGGACCGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTTGTTAGGCAAAGTCACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCGCAAACATAGCTCTA

Protein sequence

>Msa1242200

VLAGKLSTELGIKTPVDKFFKLFASELHEVQNISERVPGAKLHEGEDWHHTDSVKHWTYVIDGKVHTCHESIEVDEQNKKLTFKLFGGDIDEHYKVFNVIIEVIDNGDGSGAAKWTVEYEKINEDIDPPNGWMDLLGKVTREVDAHLVKGANIAL