AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0915680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCGACCGCCCGGGGTCGC+TGG 0.207871 6_2:-32898616 None:intergenic
CAGGTCTTAGGAAACAAATT+TGG 0.216973 6_2:+32899631 Msa0915680:CDS
ACGGAGCCGGGGCGACCGCC+CGG 0.275320 6_2:-32898625 None:intergenic
CGGAGCCGGGGCGACCGCCC+GGG 0.277719 6_2:-32898624 None:intergenic
CCACATTAGGCCCAAGCTTT+TGG 0.287770 6_2:+32898659 Msa0915680:five_prime_UTR
TCATTGGCTACTTCGGCTTC+CGG 0.318480 6_2:-32899594 None:intergenic
TTGTGCGTGATCCAGGTCTT+AGG 0.337344 6_2:+32899619 Msa0915680:CDS
GGCGACCGCCCGGGGTCGCT+GGG 0.341802 6_2:-32898615 None:intergenic
TGGGAGTATGCGGTGATGTT+TGG 0.349332 6_2:+32899651 Msa0915680:CDS
TGGAATTTGATGGAATTTCA+TGG 0.382517 6_2:+32899307 Msa0915680:exon
GCGACCCCGGGCGGTCGCCC+CGG 0.382965 6_2:+32898619 Msa0915680:five_prime_UTR
GGGAGTATGCGGTGATGTTT+GGG 0.392710 6_2:+32899652 Msa0915680:CDS
CACCGCCATTTGCTCTTTGA+CGG 0.398780 6_2:-32899726 None:intergenic
GGAGCCGGGGCGACCGCCCG+GGG 0.399005 6_2:-32898623 None:intergenic
TGCGGTGATGTTTGGGAGCT+TGG 0.404102 6_2:+32899659 Msa0915680:CDS
AGGTCTTAGGAAACAAATTT+GGG 0.408520 6_2:+32899632 Msa0915680:CDS
AATGAGATTGTGCGTGATCC+AGG 0.422235 6_2:+32899612 Msa0915680:CDS
GGTAAAGTACAGACGGAGCC+GGG 0.424499 6_2:-32898637 None:intergenic
ATGAGTTTGATGGAATTTGA+TGG 0.425202 6_2:+32899297 Msa0915680:five_prime_UTR
GAAAGCTGCTGTAGCCTTTG+TGG 0.435734 6_2:-32898757 None:intergenic
CACAATCTCATTGGCTACTT+CGG 0.444118 6_2:-32899601 None:intergenic
GACGGACGGAAGGGCAGGGG+CGG 0.457105 6_2:+32898584 None:intergenic
CTTAGGTGAGATGAGTTTGA+TGG 0.466099 6_2:+32899287 Msa0915680:five_prime_UTR
CAAATTTGTTTCCTAAGACC+TGG 0.470424 6_2:-32899630 None:intergenic
GTGTAAATGGGCCAAAAGCT+TGG 0.473146 6_2:-32898670 None:intergenic
TGTAAATGGGCCAAAAGCTT+GGG 0.473427 6_2:-32898669 None:intergenic
GAGGAACTGCAGCAGCCAGC+AGG 0.477012 6_2:-32899395 None:intergenic
CTGAAGAGTTTGATGAGCTT+AGG 0.479230 6_2:+32899270 Msa0915680:five_prime_UTR
GAATGTAAATGTTGTGGAAC+CGG 0.483743 6_2:+32899575 Msa0915680:CDS
CATTCACTCTAATTCTCTAA+AGG 0.488492 6_2:+32898882 Msa0915680:five_prime_UTR
TGGATCACGCACAATCTCAT+TGG 0.489029 6_2:-32899610 None:intergenic
GACCGCCCGGGGTCGCTGGG+CGG 0.492985 6_2:-32898612 None:intergenic
CTCTCATCAATCATCTCTCT+CGG 0.496967 6_2:+32898795 Msa0915680:five_prime_UTR
ATTAGTTCTCTCATCTCTCT+CGG 0.503929 6_2:+32898830 Msa0915680:five_prime_UTR
AATGCGAATGGAGTGCCTGC+TGG 0.517339 6_2:+32899380 Msa0915680:CDS
CATCAATCATCTCTCTCGGC+AGG 0.519196 6_2:+32898799 Msa0915680:five_prime_UTR
CCAAAAGCTTGGGCCTAATG+TGG 0.520380 6_2:-32898659 None:intergenic
TATATCCGTCAAAGAGCAAA+TGG 0.527675 6_2:+32899721 Msa0915680:CDS
GGGTAAAGTACAGACGGAGC+CGG 0.549020 6_2:-32898638 None:intergenic
AGCAGGCACTCCATTCGCAT+TGG 0.552459 6_2:-32899378 None:intergenic
TTTCCGTTGACCAATGCGAA+TGG 0.563299 6_2:+32899368 Msa0915680:CDS
GTTCCTCCTCAATTGAATCT+CGG 0.564630 6_2:+32899411 Msa0915680:CDS
GTCTGTACTTTACCCACATT+AGG 0.583824 6_2:+32898646 Msa0915680:five_prime_UTR
ACTCCATTCGCATTGGTCAA+CGG 0.585642 6_2:-32899371 None:intergenic
TTCTCACCGAGATTCAATTG+AGG 0.591935 6_2:-32899417 None:intergenic
GATTGAGAATGTAAATGTTG+TGG 0.610973 6_2:+32899569 Msa0915680:CDS
CAAAAGCTTGGGCCTAATGT+GGG 0.615109 6_2:-32898658 None:intergenic
GAAACAAATTTGGGAGTATG+CGG 0.620477 6_2:+32899641 Msa0915680:CDS
TCACCGAGATTCAATTGAGG+AGG 0.621589 6_2:-32899414 None:intergenic
TACCGCCCAGCGACCCCGGG+CGG 0.630814 6_2:+32898610 Msa0915680:five_prime_UTR
TGAATGAGAGATTAGCAACA+AGG 0.630982 6_2:-32898865 None:intergenic
TAATGTGGGTAAAGTACAGA+CGG 0.638044 6_2:-32898644 None:intergenic
GTAAAGTACAGACGGAGCCG+GGG 0.678767 6_2:-32898636 None:intergenic
AGTAATTCACAACACCACAA+AGG 0.700809 6_2:+32898743 Msa0915680:five_prime_UTR
ATCCGTCAAAGAGCAAATGG+CGG 0.716513 6_2:+32899724 Msa0915680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGAAAATTGTAAAAT+TGG + chr6_2:32898945-32898964 Msa0915680:intron 10.0%
!!! TATATTTATTTGTTTGTTTT+TGG + chr6_2:32899228-32899247 Msa0915680:intron 10.0%
!!! TTTTATGAAAATTGTAAAAT+TGG + chr6_2:32898945-32898964 Msa0915680:intron 10.0%
!!! TATATTTATTTGTTTGTTTT+TGG + chr6_2:32899228-32899247 Msa0915680:intron 10.0%
!!! GTTGAAAAGTATTTTTTTTT+TGG + chr6_2:32899462-32899481 Msa0915680:intron 15.0%
!!! GTTGAAAAGTATTTTTTTTT+TGG + chr6_2:32899462-32899481 Msa0915680:intron 15.0%
!!! TTTTTTTCAAGTGTGTAAAT+GGG - chr6_2:32898685-32898704 None:intergenic 20.0%
!!! TTTTTTTTCAAGTGTGTAAA+TGG - chr6_2:32898686-32898705 None:intergenic 20.0%
!! TACACACTTGAAAAAAAAAA+TGG + chr6_2:32898688-32898707 Msa0915680:five_prime_UTR 20.0%
!! ACACACTTGAAAAAAAAAAT+GGG + chr6_2:32898689-32898708 Msa0915680:five_prime_UTR 20.0%
!!! GTTAGTTTAATTTGAAGTTT+TGG + chr6_2:32899037-32899056 Msa0915680:intron 20.0%
!! TTTATTTCGATTTACTCAAA+TGG + chr6_2:32899167-32899186 Msa0915680:intron 20.0%
!! TTATTTCGATTTACTCAAAT+GGG + chr6_2:32899168-32899187 Msa0915680:intron 20.0%
!!! TTATTTGTTTGTTTTTGGAT+TGG + chr6_2:32899233-32899252 Msa0915680:intron 20.0%
!!! ACACATTTTCTTGATTAATT+AGG + chr6_2:32899439-32899458 Msa0915680:intron 20.0%
!!! CACATTTTCTTGATTAATTA+GGG + chr6_2:32899440-32899459 Msa0915680:intron 20.0%
!!! GGATGATTTTGTTTTAATTT+AGG + chr6_2:32899483-32899502 Msa0915680:intron 20.0%
!!! TTTTTTTCAAGTGTGTAAAT+GGG - chr6_2:32898685-32898704 None:intergenic 20.0%
!!! TTTTTTTTCAAGTGTGTAAA+TGG - chr6_2:32898686-32898705 None:intergenic 20.0%
!! TACACACTTGAAAAAAAAAA+TGG + chr6_2:32898688-32898707 Msa0915680:five_prime_UTR 20.0%
!! ACACACTTGAAAAAAAAAAT+GGG + chr6_2:32898689-32898708 Msa0915680:five_prime_UTR 20.0%
!!! GTTAGTTTAATTTGAAGTTT+TGG + chr6_2:32899037-32899056 Msa0915680:intron 20.0%
!! TTTATTTCGATTTACTCAAA+TGG + chr6_2:32899167-32899186 Msa0915680:intron 20.0%
!! TTATTTCGATTTACTCAAAT+GGG + chr6_2:32899168-32899187 Msa0915680:intron 20.0%
!!! TTATTTGTTTGTTTTTGGAT+TGG + chr6_2:32899233-32899252 Msa0915680:intron 20.0%
!!! ACACATTTTCTTGATTAATT+AGG + chr6_2:32899439-32899458 Msa0915680:intron 20.0%
!!! CACATTTTCTTGATTAATTA+GGG + chr6_2:32899440-32899459 Msa0915680:intron 20.0%
!!! GGATGATTTTGTTTTAATTT+AGG + chr6_2:32899483-32899502 Msa0915680:intron 20.0%
! CACACTTGAAAAAAAAAATG+GGG + chr6_2:32898690-32898709 Msa0915680:five_prime_UTR 25.0%
! TTTCATAAAACCCTAAAACA+AGG - chr6_2:32898935-32898954 None:intergenic 25.0%
! AAAACAAAAAACAACCAGAA+GGG - chr6_2:32899149-32899168 None:intergenic 25.0%
! AAAAACAAAAAACAACCAGA+AGG - chr6_2:32899150-32899169 None:intergenic 25.0%
!! TTTTCTTATGTGGTAAAGAT+TGG + chr6_2:32899194-32899213 Msa0915680:intron 25.0%
!!! TTTTGGATTGGTTGATTTTT+AGG + chr6_2:32899245-32899264 Msa0915680:intron 25.0%
! CACACTTGAAAAAAAAAATG+GGG + chr6_2:32898690-32898709 Msa0915680:five_prime_UTR 25.0%
! TTTCATAAAACCCTAAAACA+AGG - chr6_2:32898935-32898954 None:intergenic 25.0%
! AAAACAAAAAACAACCAGAA+GGG - chr6_2:32899149-32899168 None:intergenic 25.0%
! AAAAACAAAAAACAACCAGA+AGG - chr6_2:32899150-32899169 None:intergenic 25.0%
!! TTTTCTTATGTGGTAAAGAT+TGG + chr6_2:32899194-32899213 Msa0915680:intron 25.0%
!!! TTTTGGATTGGTTGATTTTT+AGG + chr6_2:32899245-32899264 Msa0915680:intron 25.0%
CATTCACTCTAATTCTCTAA+AGG + chr6_2:32898882-32898901 Msa0915680:five_prime_UTR 30.0%
TAAAACAAGGAAAGAAAGGA+AGG - chr6_2:32898922-32898941 None:intergenic 30.0%
!!! TTCCTTTCTTTCCTTGTTTT+AGG + chr6_2:32898921-32898940 Msa0915680:intron 30.0%
!!! TCCTTTCTTTCCTTGTTTTA+GGG + chr6_2:32898922-32898941 Msa0915680:intron 30.0%
AAACAAAAAACAACCAGAAG+GGG - chr6_2:32899148-32899167 None:intergenic 30.0%
!! AAATGGGTCATTTTCTTATG+TGG + chr6_2:32899184-32899203 Msa0915680:intron 30.0%
ATGAGTTTGATGGAATTTGA+TGG + chr6_2:32899297-32899316 Msa0915680:five_prime_UTR 30.0%
TGGAATTTGATGGAATTTCA+TGG + chr6_2:32899307-32899326 Msa0915680:exon 30.0%
!! ATGGAGTTTTATGAGTTTGA+TGG + chr6_2:32899326-32899345 Msa0915680:CDS 30.0%
!! GTTTTATGAGTTTGATGGAT+TGG + chr6_2:32899331-32899350 Msa0915680:CDS 30.0%
! TGTTTTCGTTAGATAGAACA+AGG + chr6_2:32899547-32899566 Msa0915680:intron 30.0%
GATTGAGAATGTAAATGTTG+TGG + chr6_2:32899569-32899588 Msa0915680:CDS 30.0%
AGGTCTTAGGAAACAAATTT+GGG + chr6_2:32899632-32899651 Msa0915680:CDS 30.0%
CATTCACTCTAATTCTCTAA+AGG + chr6_2:32898882-32898901 Msa0915680:five_prime_UTR 30.0%
TAAAACAAGGAAAGAAAGGA+AGG - chr6_2:32898922-32898941 None:intergenic 30.0%
!!! TTCCTTTCTTTCCTTGTTTT+AGG + chr6_2:32898921-32898940 Msa0915680:intron 30.0%
!!! TCCTTTCTTTCCTTGTTTTA+GGG + chr6_2:32898922-32898941 Msa0915680:intron 30.0%
AAACAAAAAACAACCAGAAG+GGG - chr6_2:32899148-32899167 None:intergenic 30.0%
!! AAATGGGTCATTTTCTTATG+TGG + chr6_2:32899184-32899203 Msa0915680:intron 30.0%
ATGAGTTTGATGGAATTTGA+TGG + chr6_2:32899297-32899316 Msa0915680:five_prime_UTR 30.0%
TGGAATTTGATGGAATTTCA+TGG + chr6_2:32899307-32899326 Msa0915680:exon 30.0%
!! ATGGAGTTTTATGAGTTTGA+TGG + chr6_2:32899326-32899345 Msa0915680:CDS 30.0%
!! GTTTTATGAGTTTGATGGAT+TGG + chr6_2:32899331-32899350 Msa0915680:CDS 30.0%
! TGTTTTCGTTAGATAGAACA+AGG + chr6_2:32899547-32899566 Msa0915680:intron 30.0%
GATTGAGAATGTAAATGTTG+TGG + chr6_2:32899569-32899588 Msa0915680:CDS 30.0%
AGGTCTTAGGAAACAAATTT+GGG + chr6_2:32899632-32899651 Msa0915680:CDS 30.0%
TAATGTGGGTAAAGTACAGA+CGG - chr6_2:32898647-32898666 None:intergenic 35.0%
AGTAATTCACAACACCACAA+AGG + chr6_2:32898743-32898762 Msa0915680:five_prime_UTR 35.0%
! ATTAGTTCTCTCATCTCTCT+CGG + chr6_2:32898830-32898849 Msa0915680:five_prime_UTR 35.0%
TGAATGAGAGATTAGCAACA+AGG - chr6_2:32898868-32898887 None:intergenic 35.0%
ACCCTAAAACAAGGAAAGAA+AGG - chr6_2:32898926-32898945 None:intergenic 35.0%
GCAGACTGCAATTGAAAAAT+TGG + chr6_2:32898975-32898994 Msa0915680:intron 35.0%
GTGAGTGTGTTTACTTGATT+AGG + chr6_2:32899512-32899531 Msa0915680:intron 35.0%
GAATGTAAATGTTGTGGAAC+CGG + chr6_2:32899575-32899594 Msa0915680:CDS 35.0%
! CAAATTTGTTTCCTAAGACC+TGG - chr6_2:32899633-32899652 None:intergenic 35.0%
CAGGTCTTAGGAAACAAATT+TGG + chr6_2:32899631-32899650 Msa0915680:CDS 35.0%
! GAAACAAATTTGGGAGTATG+CGG + chr6_2:32899641-32899660 Msa0915680:CDS 35.0%
TATATCCGTCAAAGAGCAAA+TGG + chr6_2:32899721-32899740 Msa0915680:CDS 35.0%
TAATGTGGGTAAAGTACAGA+CGG - chr6_2:32898647-32898666 None:intergenic 35.0%
AGTAATTCACAACACCACAA+AGG + chr6_2:32898743-32898762 Msa0915680:five_prime_UTR 35.0%
! ATTAGTTCTCTCATCTCTCT+CGG + chr6_2:32898830-32898849 Msa0915680:five_prime_UTR 35.0%
TGAATGAGAGATTAGCAACA+AGG - chr6_2:32898868-32898887 None:intergenic 35.0%
ACCCTAAAACAAGGAAAGAA+AGG - chr6_2:32898926-32898945 None:intergenic 35.0%
GCAGACTGCAATTGAAAAAT+TGG + chr6_2:32898975-32898994 Msa0915680:intron 35.0%
GTGAGTGTGTTTACTTGATT+AGG + chr6_2:32899512-32899531 Msa0915680:intron 35.0%
GAATGTAAATGTTGTGGAAC+CGG + chr6_2:32899575-32899594 Msa0915680:CDS 35.0%
! CAAATTTGTTTCCTAAGACC+TGG - chr6_2:32899633-32899652 None:intergenic 35.0%
CAGGTCTTAGGAAACAAATT+TGG + chr6_2:32899631-32899650 Msa0915680:CDS 35.0%
! GAAACAAATTTGGGAGTATG+CGG + chr6_2:32899641-32899660 Msa0915680:CDS 35.0%
TATATCCGTCAAAGAGCAAA+TGG + chr6_2:32899721-32899740 Msa0915680:CDS 35.0%
GTCTGTACTTTACCCACATT+AGG + chr6_2:32898646-32898665 Msa0915680:five_prime_UTR 40.0%
TGTAAATGGGCCAAAAGCTT+GGG - chr6_2:32898672-32898691 None:intergenic 40.0%
CTCTCATCAATCATCTCTCT+CGG + chr6_2:32898795-32898814 Msa0915680:five_prime_UTR 40.0%
CTGAAGAGTTTGATGAGCTT+AGG + chr6_2:32899270-32899289 Msa0915680:five_prime_UTR 40.0%
CTTAGGTGAGATGAGTTTGA+TGG + chr6_2:32899287-32899306 Msa0915680:five_prime_UTR 40.0%
GTTCCTCCTCAATTGAATCT+CGG + chr6_2:32899411-32899430 Msa0915680:CDS 40.0%
TTCTCACCGAGATTCAATTG+AGG - chr6_2:32899420-32899439 None:intergenic 40.0%
! CACAATCTCATTGGCTACTT+CGG - chr6_2:32899604-32899623 None:intergenic 40.0%
GTCTGTACTTTACCCACATT+AGG + chr6_2:32898646-32898665 Msa0915680:five_prime_UTR 40.0%
TGTAAATGGGCCAAAAGCTT+GGG - chr6_2:32898672-32898691 None:intergenic 40.0%
CTCTCATCAATCATCTCTCT+CGG + chr6_2:32898795-32898814 Msa0915680:five_prime_UTR 40.0%
CTGAAGAGTTTGATGAGCTT+AGG + chr6_2:32899270-32899289 Msa0915680:five_prime_UTR 40.0%
CTTAGGTGAGATGAGTTTGA+TGG + chr6_2:32899287-32899306 Msa0915680:five_prime_UTR 40.0%
GTTCCTCCTCAATTGAATCT+CGG + chr6_2:32899411-32899430 Msa0915680:CDS 40.0%
TTCTCACCGAGATTCAATTG+AGG - chr6_2:32899420-32899439 None:intergenic 40.0%
! CACAATCTCATTGGCTACTT+CGG - chr6_2:32899604-32899623 None:intergenic 40.0%
CAAAAGCTTGGGCCTAATGT+GGG - chr6_2:32898661-32898680 None:intergenic 45.0%
GTGTAAATGGGCCAAAAGCT+TGG - chr6_2:32898673-32898692 None:intergenic 45.0%
! GAGATTTTCACAACCCCTTC+TGG + chr6_2:32899132-32899151 Msa0915680:intron 45.0%
TTTCCGTTGACCAATGCGAA+TGG + chr6_2:32899368-32899387 Msa0915680:CDS 45.0%
! ACTCCATTCGCATTGGTCAA+CGG - chr6_2:32899374-32899393 None:intergenic 45.0%
TCACCGAGATTCAATTGAGG+AGG - chr6_2:32899417-32899436 None:intergenic 45.0%
TGGATCACGCACAATCTCAT+TGG - chr6_2:32899613-32899632 None:intergenic 45.0%
AATGAGATTGTGCGTGATCC+AGG + chr6_2:32899612-32899631 Msa0915680:CDS 45.0%
ATCCGTCAAAGAGCAAATGG+CGG + chr6_2:32899724-32899743 Msa0915680:CDS 45.0%
CAAAAGCTTGGGCCTAATGT+GGG - chr6_2:32898661-32898680 None:intergenic 45.0%
GTGTAAATGGGCCAAAAGCT+TGG - chr6_2:32898673-32898692 None:intergenic 45.0%
! GAGATTTTCACAACCCCTTC+TGG + chr6_2:32899132-32899151 Msa0915680:intron 45.0%
TTTCCGTTGACCAATGCGAA+TGG + chr6_2:32899368-32899387 Msa0915680:CDS 45.0%
! ACTCCATTCGCATTGGTCAA+CGG - chr6_2:32899374-32899393 None:intergenic 45.0%
TCACCGAGATTCAATTGAGG+AGG - chr6_2:32899417-32899436 None:intergenic 45.0%
TGGATCACGCACAATCTCAT+TGG - chr6_2:32899613-32899632 None:intergenic 45.0%
AATGAGATTGTGCGTGATCC+AGG + chr6_2:32899612-32899631 Msa0915680:CDS 45.0%
ATCCGTCAAAGAGCAAATGG+CGG + chr6_2:32899724-32899743 Msa0915680:CDS 45.0%
CCAAAAGCTTGGGCCTAATG+TGG - chr6_2:32898662-32898681 None:intergenic 50.0%
! CCACATTAGGCCCAAGCTTT+TGG + chr6_2:32898659-32898678 Msa0915680:five_prime_UTR 50.0%
GAAAGCTGCTGTAGCCTTTG+TGG - chr6_2:32898760-32898779 None:intergenic 50.0%
CATCAATCATCTCTCTCGGC+AGG + chr6_2:32898799-32898818 Msa0915680:five_prime_UTR 50.0%
! TCATTGGCTACTTCGGCTTC+CGG - chr6_2:32899597-32899616 None:intergenic 50.0%
TTGTGCGTGATCCAGGTCTT+AGG + chr6_2:32899619-32899638 Msa0915680:CDS 50.0%
! TGGGAGTATGCGGTGATGTT+TGG + chr6_2:32899651-32899670 Msa0915680:CDS 50.0%
! GGGAGTATGCGGTGATGTTT+GGG + chr6_2:32899652-32899671 Msa0915680:CDS 50.0%
CACCGCCATTTGCTCTTTGA+CGG - chr6_2:32899729-32899748 None:intergenic 50.0%
CCAAAAGCTTGGGCCTAATG+TGG - chr6_2:32898662-32898681 None:intergenic 50.0%
! CCACATTAGGCCCAAGCTTT+TGG + chr6_2:32898659-32898678 Msa0915680:five_prime_UTR 50.0%
GAAAGCTGCTGTAGCCTTTG+TGG - chr6_2:32898760-32898779 None:intergenic 50.0%
CATCAATCATCTCTCTCGGC+AGG + chr6_2:32898799-32898818 Msa0915680:five_prime_UTR 50.0%
! TCATTGGCTACTTCGGCTTC+CGG - chr6_2:32899597-32899616 None:intergenic 50.0%
TTGTGCGTGATCCAGGTCTT+AGG + chr6_2:32899619-32899638 Msa0915680:CDS 50.0%
! TGGGAGTATGCGGTGATGTT+TGG + chr6_2:32899651-32899670 Msa0915680:CDS 50.0%
! GGGAGTATGCGGTGATGTTT+GGG + chr6_2:32899652-32899671 Msa0915680:CDS 50.0%
CACCGCCATTTGCTCTTTGA+CGG - chr6_2:32899729-32899748 None:intergenic 50.0%
GTAAAGTACAGACGGAGCCG+GGG - chr6_2:32898639-32898658 None:intergenic 55.0%
GGTAAAGTACAGACGGAGCC+GGG - chr6_2:32898640-32898659 None:intergenic 55.0%
GGGTAAAGTACAGACGGAGC+CGG - chr6_2:32898641-32898660 None:intergenic 55.0%
AGCAGGCACTCCATTCGCAT+TGG - chr6_2:32899381-32899400 None:intergenic 55.0%
! AATGCGAATGGAGTGCCTGC+TGG + chr6_2:32899380-32899399 Msa0915680:CDS 55.0%
TGCGGTGATGTTTGGGAGCT+TGG + chr6_2:32899659-32899678 Msa0915680:CDS 55.0%
GTAAAGTACAGACGGAGCCG+GGG - chr6_2:32898639-32898658 None:intergenic 55.0%
GGTAAAGTACAGACGGAGCC+GGG - chr6_2:32898640-32898659 None:intergenic 55.0%
GGGTAAAGTACAGACGGAGC+CGG - chr6_2:32898641-32898660 None:intergenic 55.0%
AGCAGGCACTCCATTCGCAT+TGG - chr6_2:32899381-32899400 None:intergenic 55.0%
! AATGCGAATGGAGTGCCTGC+TGG + chr6_2:32899380-32899399 Msa0915680:CDS 55.0%
TGCGGTGATGTTTGGGAGCT+TGG + chr6_2:32899659-32899678 Msa0915680:CDS 55.0%
! GTTTTACCGCCCAGCGACCC+CGG + chr6_2:32898606-32898625 Msa0915680:five_prime_UTR 65.0%
! TTTTACCGCCCAGCGACCCC+GGG + chr6_2:32898607-32898626 Msa0915680:five_prime_UTR 65.0%
GAGGAACTGCAGCAGCCAGC+AGG - chr6_2:32899398-32899417 None:intergenic 65.0%
! GTTTTACCGCCCAGCGACCC+CGG + chr6_2:32898606-32898625 Msa0915680:five_prime_UTR 65.0%
! TTTTACCGCCCAGCGACCCC+GGG + chr6_2:32898607-32898626 Msa0915680:five_prime_UTR 65.0%
GAGGAACTGCAGCAGCCAGC+AGG - chr6_2:32899398-32899417 None:intergenic 65.0%
! TACCGCCCAGCGACCCCGGG+CGG + chr6_2:32898610-32898629 Msa0915680:five_prime_UTR 80.0%
! TACCGCCCAGCGACCCCGGG+CGG + chr6_2:32898610-32898629 Msa0915680:five_prime_UTR 80.0%
! GACCGCCCGGGGTCGCTGGG+CGG - chr6_2:32898615-32898634 None:intergenic 85.0%
! GGCGACCGCCCGGGGTCGCT+GGG - chr6_2:32898618-32898637 None:intergenic 85.0%
! ACGGAGCCGGGGCGACCGCC+CGG - chr6_2:32898628-32898647 None:intergenic 85.0%
! GACCGCCCGGGGTCGCTGGG+CGG - chr6_2:32898615-32898634 None:intergenic 85.0%
! GGCGACCGCCCGGGGTCGCT+GGG - chr6_2:32898618-32898637 None:intergenic 85.0%
! ACGGAGCCGGGGCGACCGCC+CGG - chr6_2:32898628-32898647 None:intergenic 85.0%
! GGGCGACCGCCCGGGGTCGC+TGG - chr6_2:32898619-32898638 None:intergenic 90.0%
! GCGACCCCGGGCGGTCGCCC+CGG + chr6_2:32898619-32898638 Msa0915680:five_prime_UTR 90.0%
! GGAGCCGGGGCGACCGCCCG+GGG - chr6_2:32898626-32898645 None:intergenic 90.0%
! CGGAGCCGGGGCGACCGCCC+GGG - chr6_2:32898627-32898646 None:intergenic 90.0%
! GGGCGACCGCCCGGGGTCGC+TGG - chr6_2:32898619-32898638 None:intergenic 90.0%
! GCGACCCCGGGCGGTCGCCC+CGG + chr6_2:32898619-32898638 Msa0915680:five_prime_UTR 90.0%
! GGAGCCGGGGCGACCGCCCG+GGG - chr6_2:32898626-32898645 None:intergenic 90.0%
! CGGAGCCGGGGCGACCGCCC+GGG - chr6_2:32898627-32898646 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 32898597 32899749 32898597 ID=Msa0915680;Name=Msa0915680
chr6_2 mRNA 32898597 32899749 32898597 ID=Msa0915680-mRNA-1;Parent=Msa0915680;Name=Msa0915680-mRNA-1;_AED=0.10;_eAED=0.10;_QI=366|0.5|0.66|1|0.5|0.33|3|0|97
chr6_2 exon 32898597 32898903 32898597 ID=Msa0915680-mRNA-1:exon:5837;Parent=Msa0915680-mRNA-1
chr6_2 exon 32899267 32899432 32899267 ID=Msa0915680-mRNA-1:exon:5838;Parent=Msa0915680-mRNA-1
chr6_2 exon 32899563 32899749 32899563 ID=Msa0915680-mRNA-1:exon:5839;Parent=Msa0915680-mRNA-1
chr6_2 five_prime_UTR 32898597 32898903 32898597 ID=Msa0915680-mRNA-1:five_prime_utr;Parent=Msa0915680-mRNA-1
chr6_2 five_prime_UTR 32899267 32899325 32899267 ID=Msa0915680-mRNA-1:five_prime_utr;Parent=Msa0915680-mRNA-1
chr6_2 CDS 32899326 32899432 32899326 ID=Msa0915680-mRNA-1:cds;Parent=Msa0915680-mRNA-1
chr6_2 CDS 32899563 32899749 32899563 ID=Msa0915680-mRNA-1:cds;Parent=Msa0915680-mRNA-1
Gene Sequence

>Msa0915680

ATGGAGTTTTATGAGTTTGATGGATTGGTTGCTAGTAAAAGTTTTCCGTTGACCAATGCGAATGGAGTGCCTGCTGGCTGCTGCAGTTCCTCCTCAATTGAATCTCGAACAAGGATTGAGAATGTAAATGTTGTGGAACCGGAAGCCGAAGTAGCCAATGAGATTGTGCGTGATCCAGGTCTTAGGAAACAAATTTGGGAGTATGCGGTGATGTTTGGGAGCTTGGTGATAAACAATTCTCTGTGCTTATTGATGAGTCACGTGATATATCCGTCAAAGAGCAAATGGCGGTGA
Protein sequence

>Msa0915680

MEFYEFDGLVASKSFPLTNANGVPAGCCSSSSIESRTRIENVNVVEPEAEVANEIVRDPGLRKQIWEYAVMFGSLVINNSLCLLMSHVIYPSKSKWR*