AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0342750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342750 MtrunA17_Chr6g0487501 39.496 119 65 2 81 192 33 151 8.86e-20 82.4
Msa0342750 MtrunA17_Chr3g0092521 30.088 226 127 9 3 205 2 219 2.74e-19 83.6
Msa0342750 MtrunA17_Chr4g0060621 30.675 163 101 6 49 207 64 218 5.21e-16 74.7
Msa0342750 MtrunA17_Chr4g0064221 26.190 210 124 9 17 214 140 330 9.03e-13 66.2
Msa0342750 MtrunA17_Chr6g0487861 41.096 73 43 0 49 121 9 81 1.42e-11 58.5
Msa0342750 MtrunA17_Chr6g0487691 41.096 73 43 0 49 121 9 81 1.42e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342750 AT1G74560.1 32.487 197 113 7 17 208 45 226 9.11e-21 87.8
Msa0342750 AT1G74560.3 32.000 175 102 5 17 189 45 204 5.47e-18 80.5
Msa0342750 AT1G18800.1 29.744 195 115 7 17 205 41 219 2.47e-17 78.6

Find 38 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACATGGATTCTGGTTTA+AGG 0.177881 3_1:-709153 Msa0342750:CDS
GATTATTATTTGTTTGATTA+TGG 0.226047 3_1:-708526 Msa0342750:three_prime_UTR
CAGATGCATTTGAGATGTTT+TGG 0.252559 3_1:-708749 Msa0342750:CDS
TTTCGCGCTTACATGGATTC+TGG 0.322014 3_1:-709160 Msa0342750:CDS
ATTCTGGTTTAAGGCGATTA+AGG 0.325501 3_1:-709144 Msa0342750:CDS
TAAATTAAATGTTCTTTGTT+TGG 0.338500 3_1:-708497 Msa0342750:three_prime_UTR
CTGAATCTATTGCATTCAAC+TGG 0.388969 3_1:+709102 None:intergenic
CTCTCTTCACAAACAATGTC+GGG 0.397057 3_1:+708888 None:intergenic
TTCAACTGGCGACCAATGTC+TGG 0.403365 3_1:+709116 None:intergenic
TTCTGCTATATGACAACAAT+AGG 0.406928 3_1:+709601 None:intergenic
GAACATATTTCGCGCTTACA+TGG 0.441207 3_1:-709167 Msa0342750:CDS
TGAAGAGAGAGATGAGGATG+AGG 0.446237 3_1:-708874 Msa0342750:CDS
AAACGAAAACAGGAAGGAAA+AGG 0.458050 3_1:+709630 None:intergenic
CACGTTCAATCTTCTCCACC+CGG 0.468969 3_1:+709246 None:intergenic
GTGGTTTGAAATTGAGATGC+GGG 0.475190 3_1:-709528 Msa0342750:CDS
AATAAATGTGGTTATTATTG+AGG 0.481101 3_1:-708441 None:intergenic
TAGGAATCAGAAACGAAAAC+AGG 0.501546 3_1:+709620 None:intergenic
GGGATGGATGTTTCTGATGA+GGG 0.503789 3_1:-709554 Msa0342750:CDS
CTGAATCTTCATGCATACAT+TGG 0.523603 3_1:-708923 Msa0342750:CDS
AGGGATGGATGTTTCTGATG+AGG 0.538812 3_1:-709555 Msa0342750:exon
TCTCTCTTCACAAACAATGT+CGG 0.541509 3_1:+708887 None:intergenic
AGAGAGAGATGAGGATGAGG+AGG 0.543196 3_1:-708871 Msa0342750:CDS
TTGACATTCTGAAAGAGGGA+TGG 0.556960 3_1:-709570 Msa0342750:exon
ATTATTTGTTTGAATAAATG+TGG 0.560942 3_1:-708453 Msa0342750:three_prime_UTR
ATGTTTCTGATGAGGGAAGG+TGG 0.567771 3_1:-709547 Msa0342750:CDS
CAACTCTTCTTCATCAGATG+TGG 0.573742 3_1:+708791 None:intergenic
TGAAACCATATCAGGTTCAG+AGG 0.575619 3_1:-709657 None:intergenic
GATGAAGAAGAGTTGAGTGA+TGG 0.593224 3_1:-708783 Msa0342750:CDS
TGGATGTTTCTGATGAGGGA+AGG 0.596404 3_1:-709550 Msa0342750:CDS
GACACAGAGGATGAAGTTGA+TGG 0.599003 3_1:-708690 Msa0342750:CDS
TCACCTTGTAGATACTTCAA+AGG 0.607832 3_1:+708720 None:intergenic
ATTAAGGCTCATCCAGACAT+TGG 0.614087 3_1:-709128 Msa0342750:CDS
GATCCTTTGAAGTATCTACA+AGG 0.624256 3_1:-708723 Msa0342750:CDS
GGTGGTTTGAAATTGAGATG+CGG 0.640779 3_1:-709529 Msa0342750:CDS
AATCAGAAACGAAAACAGGA+AGG 0.656656 3_1:+709624 None:intergenic
TGTTTGTGAAGAGAGAGATG+AGG 0.664206 3_1:-708880 Msa0342750:CDS
GATGAGGATGAGGAGGACGA+CGG 0.699737 3_1:-708864 Msa0342750:CDS
AGGTGATGATGATGACACAG+AGG 0.802099 3_1:-708703 Msa0342750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTAGTTTATTTAATAT+GGG + chr3_1:708765-708784 None:intergenic 10.0%
!!! TTAAGTTAGTTTATTTAATA+TGG + chr3_1:708766-708785 None:intergenic 10.0%
!!! TAAGTTAGTTTATTTAATAT+GGG + chr3_1:708765-708784 None:intergenic 10.0%
!!! TTAAGTTAGTTTATTTAATA+TGG + chr3_1:708766-708785 None:intergenic 10.0%
!! TCATTAAAAAAAAAAACACT+TGG + chr3_1:708616-708635 None:intergenic 15.0%
!! AATAAAATCAAGGATTTATA+AGG + chr3_1:708731-708750 None:intergenic 15.0%
!!! TTTTTCATATACAATTTCAA+TGG + chr3_1:709034-709053 None:intergenic 15.0%
!!! TATTAGTTGTTTTTTGATTA+TGG - chr3_1:709508-709527 Msa0342750:CDS 15.0%
!! GATTATTATTTGTTTGATTA+TGG - chr3_1:709560-709579 Msa0342750:exon 15.0%
!! TAAATTAAATGTTCTTTGTT+TGG - chr3_1:709589-709608 Msa0342750:five_prime_UTR 15.0%
!! ATTATTTGTTTGAATAAATG+TGG - chr3_1:709633-709652 Msa0342750:five_prime_UTR 15.0%
!! TCATTAAAAAAAAAAACACT+TGG + chr3_1:708616-708635 None:intergenic 15.0%
!! AATAAAATCAAGGATTTATA+AGG + chr3_1:708731-708750 None:intergenic 15.0%
!!! TTTTTCATATACAATTTCAA+TGG + chr3_1:709034-709053 None:intergenic 15.0%
!!! TATTAGTTGTTTTTTGATTA+TGG - chr3_1:709508-709527 Msa0342750:CDS 15.0%
!! GATTATTATTTGTTTGATTA+TGG - chr3_1:709560-709579 Msa0342750:exon 15.0%
!! TAAATTAAATGTTCTTTGTT+TGG - chr3_1:709589-709608 Msa0342750:five_prime_UTR 15.0%
!! ATTATTTGTTTGAATAAATG+TGG - chr3_1:709633-709652 Msa0342750:five_prime_UTR 15.0%
!! AAAAAAAAAACACTTGGATA+CGG + chr3_1:708610-708629 None:intergenic 20.0%
!!! TATTATTATTTTAGACTCTC+CGG - chr3_1:708821-708840 Msa0342750:CDS 20.0%
!!! GAAAATGAATATTTTTCCAA+TGG - chr3_1:709093-709112 Msa0342750:CDS 20.0%
!! AAAAAAAAAACACTTGGATA+CGG + chr3_1:708610-708629 None:intergenic 20.0%
!!! TATTATTATTTTAGACTCTC+CGG - chr3_1:708821-708840 Msa0342750:CDS 20.0%
!!! GAAAATGAATATTTTTCCAA+TGG - chr3_1:709093-709112 Msa0342750:CDS 20.0%
! AAACCAAGAAAAATATGATG+AGG - chr3_1:708582-708601 Msa0342750:three_prime_UTR 25.0%
! CTTAGAGTTCAATAAAATCA+AGG + chr3_1:708741-708760 None:intergenic 25.0%
!!! ATTATTATTTTAGACTCTCC+GGG - chr3_1:708822-708841 Msa0342750:CDS 25.0%
! TATGAAAAAGATTCAGAATC+AGG - chr3_1:709045-709064 Msa0342750:CDS 25.0%
! GAACCAATTGAAAAAACTTT+GGG + chr3_1:709271-709290 None:intergenic 25.0%
! TGAACCAATTGAAAAAACTT+TGG + chr3_1:709272-709291 None:intergenic 25.0%
! AAACCAAGAAAAATATGATG+AGG - chr3_1:708582-708601 Msa0342750:three_prime_UTR 25.0%
! CTTAGAGTTCAATAAAATCA+AGG + chr3_1:708741-708760 None:intergenic 25.0%
!!! ATTATTATTTTAGACTCTCC+GGG - chr3_1:708822-708841 Msa0342750:CDS 25.0%
! TATGAAAAAGATTCAGAATC+AGG - chr3_1:709045-709064 Msa0342750:CDS 25.0%
! GAACCAATTGAAAAAACTTT+GGG + chr3_1:709271-709290 None:intergenic 25.0%
! TGAACCAATTGAAAAAACTT+TGG + chr3_1:709272-709291 None:intergenic 25.0%
TTCTGCTATATGACAACAAT+AGG + chr3_1:708488-708507 None:intergenic 30.0%
!! AAGTTTTGACATTCTGAAAG+AGG - chr3_1:708511-708530 Msa0342750:three_prime_UTR 30.0%
!! AGTTTTGACATTCTGAAAGA+GGG - chr3_1:708512-708531 Msa0342750:three_prime_UTR 30.0%
! GTACCTCATCATATTTTTCT+TGG + chr3_1:708588-708607 None:intergenic 30.0%
TTCTGCTATATGACAACAAT+AGG + chr3_1:708488-708507 None:intergenic 30.0%
!! AAGTTTTGACATTCTGAAAG+AGG - chr3_1:708511-708530 Msa0342750:three_prime_UTR 30.0%
!! AGTTTTGACATTCTGAAAGA+GGG - chr3_1:708512-708531 Msa0342750:three_prime_UTR 30.0%
! GTACCTCATCATATTTTTCT+TGG + chr3_1:708588-708607 None:intergenic 30.0%
AAACGAAAACAGGAAGGAAA+AGG + chr3_1:708459-708478 None:intergenic 35.0%
AATCAGAAACGAAAACAGGA+AGG + chr3_1:708465-708484 None:intergenic 35.0%
TAGGAATCAGAAACGAAAAC+AGG + chr3_1:708469-708488 None:intergenic 35.0%
!! CTTACATGGATTCTGGTTTA+AGG - chr3_1:708933-708952 Msa0342750:CDS 35.0%
!! ATTCTGGTTTAAGGCGATTA+AGG - chr3_1:708942-708961 Msa0342750:CDS 35.0%
CTGAATCTATTGCATTCAAC+TGG + chr3_1:708987-709006 None:intergenic 35.0%
CTGAATCTTCATGCATACAT+TGG - chr3_1:709163-709182 Msa0342750:CDS 35.0%
TCTCTCTTCACAAACAATGT+CGG + chr3_1:709202-709221 None:intergenic 35.0%
CCAATTGAAAAAACTTTGGG+AGG + chr3_1:709268-709287 None:intergenic 35.0%
!!! CCTCCCAAAGTTTTTTCAAT+TGG - chr3_1:709265-709284 Msa0342750:intron 35.0%
!! CAGATGCATTTGAGATGTTT+TGG - chr3_1:709337-709356 Msa0342750:intron 35.0%
!! GATCCTTTGAAGTATCTACA+AGG - chr3_1:709363-709382 Msa0342750:intron 35.0%
TCACCTTGTAGATACTTCAA+AGG + chr3_1:709369-709388 None:intergenic 35.0%
AAACGAAAACAGGAAGGAAA+AGG + chr3_1:708459-708478 None:intergenic 35.0%
AATCAGAAACGAAAACAGGA+AGG + chr3_1:708465-708484 None:intergenic 35.0%
TAGGAATCAGAAACGAAAAC+AGG + chr3_1:708469-708488 None:intergenic 35.0%
!! CTTACATGGATTCTGGTTTA+AGG - chr3_1:708933-708952 Msa0342750:CDS 35.0%
!! ATTCTGGTTTAAGGCGATTA+AGG - chr3_1:708942-708961 Msa0342750:CDS 35.0%
CTGAATCTATTGCATTCAAC+TGG + chr3_1:708987-709006 None:intergenic 35.0%
CTGAATCTTCATGCATACAT+TGG - chr3_1:709163-709182 Msa0342750:CDS 35.0%
TCTCTCTTCACAAACAATGT+CGG + chr3_1:709202-709221 None:intergenic 35.0%
CCAATTGAAAAAACTTTGGG+AGG + chr3_1:709268-709287 None:intergenic 35.0%
!!! CCTCCCAAAGTTTTTTCAAT+TGG - chr3_1:709265-709284 Msa0342750:intron 35.0%
!! CAGATGCATTTGAGATGTTT+TGG - chr3_1:709337-709356 Msa0342750:intron 35.0%
!! GATCCTTTGAAGTATCTACA+AGG - chr3_1:709363-709382 Msa0342750:intron 35.0%
TCACCTTGTAGATACTTCAA+AGG + chr3_1:709369-709388 None:intergenic 35.0%
! TTGACATTCTGAAAGAGGGA+TGG - chr3_1:708516-708535 Msa0342750:three_prime_UTR 40.0%
! GGTGGTTTGAAATTGAGATG+CGG - chr3_1:708557-708576 Msa0342750:three_prime_UTR 40.0%
! GTGGTTTGAAATTGAGATGC+GGG - chr3_1:708558-708577 Msa0342750:three_prime_UTR 40.0%
!!! ATTATTTTAGACTCTCCGGG+TGG - chr3_1:708825-708844 Msa0342750:CDS 40.0%
GAACATATTTCGCGCTTACA+TGG - chr3_1:708919-708938 Msa0342750:CDS 40.0%
ATTAAGGCTCATCCAGACAT+TGG - chr3_1:708958-708977 Msa0342750:CDS 40.0%
ACGCTTAAAAATGCGACCAT+TGG + chr3_1:709112-709131 None:intergenic 40.0%
CTCTCTTCACAAACAATGTC+GGG + chr3_1:709201-709220 None:intergenic 40.0%
TGTTTGTGAAGAGAGAGATG+AGG - chr3_1:709206-709225 Msa0342750:CDS 40.0%
CAACTCTTCTTCATCAGATG+TGG + chr3_1:709298-709317 None:intergenic 40.0%
GATGAAGAAGAGTTGAGTGA+TGG - chr3_1:709303-709322 Msa0342750:intron 40.0%
! TTGACATTCTGAAAGAGGGA+TGG - chr3_1:708516-708535 Msa0342750:three_prime_UTR 40.0%
! GGTGGTTTGAAATTGAGATG+CGG - chr3_1:708557-708576 Msa0342750:three_prime_UTR 40.0%
! GTGGTTTGAAATTGAGATGC+GGG - chr3_1:708558-708577 Msa0342750:three_prime_UTR 40.0%
!!! ATTATTTTAGACTCTCCGGG+TGG - chr3_1:708825-708844 Msa0342750:CDS 40.0%
GAACATATTTCGCGCTTACA+TGG - chr3_1:708919-708938 Msa0342750:CDS 40.0%
ATTAAGGCTCATCCAGACAT+TGG - chr3_1:708958-708977 Msa0342750:CDS 40.0%
ACGCTTAAAAATGCGACCAT+TGG + chr3_1:709112-709131 None:intergenic 40.0%
CTCTCTTCACAAACAATGTC+GGG + chr3_1:709201-709220 None:intergenic 40.0%
TGTTTGTGAAGAGAGAGATG+AGG - chr3_1:709206-709225 Msa0342750:CDS 40.0%
CAACTCTTCTTCATCAGATG+TGG + chr3_1:709298-709317 None:intergenic 40.0%
GATGAAGAAGAGTTGAGTGA+TGG - chr3_1:709303-709322 Msa0342750:intron 40.0%
! AGGGATGGATGTTTCTGATG+AGG - chr3_1:708531-708550 Msa0342750:three_prime_UTR 45.0%
! GGGATGGATGTTTCTGATGA+GGG - chr3_1:708532-708551 Msa0342750:three_prime_UTR 45.0%
! TGGATGTTTCTGATGAGGGA+AGG - chr3_1:708536-708555 Msa0342750:three_prime_UTR 45.0%
! ATGTTTCTGATGAGGGAAGG+TGG - chr3_1:708539-708558 Msa0342750:three_prime_UTR 45.0%
TTTCGCGCTTACATGGATTC+TGG - chr3_1:708926-708945 Msa0342750:CDS 45.0%
!!! AAGCGTTTTAACTTGCGCGA+CGG - chr3_1:709126-709145 Msa0342750:CDS 45.0%
TGAAGAGAGAGATGAGGATG+AGG - chr3_1:709212-709231 Msa0342750:CDS 45.0%
! AGGTGATGATGATGACACAG+AGG - chr3_1:709383-709402 Msa0342750:intron 45.0%
GACACAGAGGATGAAGTTGA+TGG - chr3_1:709396-709415 Msa0342750:intron 45.0%
! AGGGATGGATGTTTCTGATG+AGG - chr3_1:708531-708550 Msa0342750:three_prime_UTR 45.0%
! GGGATGGATGTTTCTGATGA+GGG - chr3_1:708532-708551 Msa0342750:three_prime_UTR 45.0%
! TGGATGTTTCTGATGAGGGA+AGG - chr3_1:708536-708555 Msa0342750:three_prime_UTR 45.0%
! ATGTTTCTGATGAGGGAAGG+TGG - chr3_1:708539-708558 Msa0342750:three_prime_UTR 45.0%
TTTCGCGCTTACATGGATTC+TGG - chr3_1:708926-708945 Msa0342750:CDS 45.0%
!!! AAGCGTTTTAACTTGCGCGA+CGG - chr3_1:709126-709145 Msa0342750:CDS 45.0%
TGAAGAGAGAGATGAGGATG+AGG - chr3_1:709212-709231 Msa0342750:CDS 45.0%
! AGGTGATGATGATGACACAG+AGG - chr3_1:709383-709402 Msa0342750:intron 45.0%
GACACAGAGGATGAAGTTGA+TGG - chr3_1:709396-709415 Msa0342750:intron 45.0%
CACGTTCAATCTTCTCCACC+CGG + chr3_1:708843-708862 None:intergenic 50.0%
TTCAACTGGCGACCAATGTC+TGG + chr3_1:708973-708992 None:intergenic 50.0%
AGAGAGAGATGAGGATGAGG+AGG - chr3_1:709215-709234 Msa0342750:CDS 50.0%
CACGTTCAATCTTCTCCACC+CGG + chr3_1:708843-708862 None:intergenic 50.0%
TTCAACTGGCGACCAATGTC+TGG + chr3_1:708973-708992 None:intergenic 50.0%
AGAGAGAGATGAGGATGAGG+AGG - chr3_1:709215-709234 Msa0342750:CDS 50.0%
GATGAGGATGAGGAGGACGA+CGG - chr3_1:709222-709241 Msa0342750:CDS 55.0%
GATGAGGATGAGGAGGACGA+CGG - chr3_1:709222-709241 Msa0342750:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 708443 709665 708443 ID=Msa0342750;Name=Msa0342750
chr3_1 mRNA 708443 709665 708443 ID=Msa0342750-mRNA-1;Parent=Msa0342750;Name=Msa0342750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=92|1|1|1|1|1|2|243|218
chr3_1 exon 708443 709273 708443 ID=Msa0342750-mRNA-1:exon:133;Parent=Msa0342750-mRNA-1
chr3_1 exon 709505 709665 709505 ID=Msa0342750-mRNA-1:exon:132;Parent=Msa0342750-mRNA-1
chr3_1 five_prime_UTR 709574 709665 709574 ID=Msa0342750-mRNA-1:five_prime_utr;Parent=Msa0342750-mRNA-1
chr3_1 CDS 709505 709573 709505 ID=Msa0342750-mRNA-1:cds;Parent=Msa0342750-mRNA-1
chr3_1 CDS 708686 709273 708686 ID=Msa0342750-mRNA-1:cds;Parent=Msa0342750-mRNA-1
chr3_1 three_prime_UTR 708443 708685 708443 ID=Msa0342750-mRNA-1:three_prime_utr;Parent=Msa0342750-mRNA-1
Gene Sequence

>Msa0342750

ATGGATGTTTCTGATGAGGGAAGGTGGTTTGAAATTGAGATGCGGGAAAACCAAGAAAAATATGATGAGACTCTCCGGGTGGAGAAGATTGAACGTGCAAAAATAAATCAGAGTTTTGTTGACAAACTAAGCGATCACTGTCATAAGCGCAAAGAACATATTTCGCGCTTACATGGATTCTGGTTTAAGGCGATTAAGGCTCATCCAGACATTGGTCGCCAGTTGAATGCAATAGATTCAGAAATTTTTGAGAAACATCTCACTTCCATTGAAATTGTATATGAAAAAGATTCAGAATCAGGATTTATGATCACATTTGATTTCACTGAAAATGAATATTTTTCCAATGGTCGCATTTTTAAGCGTTTTAACTTGCGCGACGGAGATTTAATATCAACTGAATCTTCATGCATACATTGGATAAAAGATATTCCCGACATTGTTTGTGAAGAGAGAGATGAGGATGAGGAGGACGACGGTACATCTGAATCAAAACTTACCTCCCAAAGTTTTTTCAATTGGTTCACGTCCACATCTGATGAAGAAGAGTTGAGTGATGGAGATCATCTTGCAGATGCATTTGAGATGTTTTGGATTGATCCTTTGAAGTATCTACAAGGTGATGATGATGACACAGAGGATGAAGTTGATGGTTGA

Protein sequence

>Msa0342750

MDVSDEGRWFEIEMRENQEKYDETLRVEKIERAKINQSFVDKLSDHCHKRKEHISRLHGFWFKAIKAHPDIGRQLNAIDSEIFEKHLTSIEIVYEKDSESGFMITFDFTENEYFSNGRIFKRFNLRDGDLISTESSCIHWIKDIPDIVCEERDEDEEDDGTSESKLTSQSFFNWFTSTSDEEELSDGDHLADAFEMFWIDPLKYLQGDDDDTEDEVDG*