AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0935010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGACATATCTCATAAATT+TGG 0.128373 6_3:+5323716 None:intergenic
AAGTTCAATGCTTCGTATTT+TGG 0.153929 6_3:-5323770 Msa0935010:CDS
AATCTAGGAATTGCAGTTAT+AGG 0.217354 6_3:-5323432 Msa0935010:three_prime_UTR
CCTTCTTCAAACGATTGATT+TGG 0.269274 6_3:+5325440 None:intergenic
CAGGTCACTATGTCTCTTTC+CGG 0.315834 6_3:-5325216 Msa0935010:exon
CTTCTTCAAACGATTGATTT+GGG 0.329224 6_3:+5325441 None:intergenic
GCGACATATCTCATAAATTT+GGG 0.331701 6_3:+5323717 None:intergenic
GATGTATTTCAACTCCTTCT+CGG 0.372367 6_3:+5323672 None:intergenic
TCACCAAAGTTGTAAATTGC+AGG 0.382052 6_3:-5323286 Msa0935010:three_prime_UTR
TACCCTGCAATTTACAACTT+TGG 0.390649 6_3:+5323283 None:intergenic
AATATTCTTCCCCAGTCCTT+TGG 0.392166 6_3:+5323695 None:intergenic
AGCTCGGTTTGTCTCCCATT+AGG 0.411135 6_3:-5323599 Msa0935010:three_prime_UTR
CGTTTGAAGAAGGTCGGAAA+AGG 0.424466 6_3:-5325430 Msa0935010:five_prime_UTR
GAAGATCCGCCGAGCAAGAC+CGG 0.434552 6_3:+5325197 None:intergenic
GAGATATGTCGCCAAAGGAC+TGG 0.434896 6_3:-5323706 Msa0935010:CDS
AGATATGTCGCCAAAGGACT+GGG 0.435686 6_3:-5323705 Msa0935010:CDS
GGGAGACAAACCGAGCTTAG+AGG 0.439522 6_3:+5323605 None:intergenic
CCAAATCAATCGTTTGAAGA+AGG 0.458072 6_3:-5325440 Msa0935010:five_prime_UTR
TCCAACAAGCTTGGAGGAGG+GGG 0.460431 6_3:-5325132 Msa0935010:CDS
CCATTAGGACACCAAGGAAA+AGG 0.476417 6_3:-5323584 Msa0935010:three_prime_UTR
ATGAAATACCTGTGATTGTT+GGG 0.477825 6_3:+5325101 None:intergenic
ATTGATTATGTTACCGCTGT+GGG 0.485009 6_3:-5323797 Msa0935010:CDS
TATAGGTTTCTTTAGAACTT+CGG 0.490184 6_3:-5323415 Msa0935010:three_prime_UTR
GTTTCTTTAGAACTTCGGTT+TGG 0.495359 6_3:-5323410 Msa0935010:three_prime_UTR
AGGAGGGGCCCAACAATCAC+AGG 0.499979 6_3:-5325109 Msa0935010:intron
CAATCCAACAAGCTTGGAGG+AGG 0.510329 6_3:-5325135 Msa0935010:CDS
AAAAGGGTCGTGAATCCAAC+CGG 0.519654 6_3:-5325413 Msa0935010:intron
TTGCATCAATCCAACAAGCT+TGG 0.523889 6_3:-5325141 Msa0935010:CDS
AATGAAATACCTGTGATTGT+TGG 0.532174 6_3:+5325100 None:intergenic
TGTCTCTTTCCGGTCTTGCT+CGG 0.537525 6_3:-5325206 Msa0935010:CDS
ATCCAACAAGCTTGGAGGAG+GGG 0.542492 6_3:-5325133 Msa0935010:CDS
ATCAATCGTTTGAAGAAGGT+CGG 0.543534 6_3:-5325436 Msa0935010:five_prime_UTR
TATTGATTATGTTACCGCTG+TGG 0.547828 6_3:-5323798 Msa0935010:CDS
GTTTGAAGAAGGTCGGAAAA+GGG 0.549628 6_3:-5325429 Msa0935010:five_prime_UTR
CATCAATCCAACAAGCTTGG+AGG 0.550773 6_3:-5325138 Msa0935010:CDS
AAATAATGTTATGTTATACT+AGG 0.551769 6_3:+5325531 Msa0935020:intron
AATGTTATGTTATACTAGGT+CGG 0.554211 6_3:+5325535 Msa0935020:intron
AGCTTGTTGGATTGATGCAA+TGG 0.556706 6_3:+5325144 None:intergenic
TTCAACACTTCAACCTCCTA+CGG 0.560993 6_3:+5323317 None:intergenic
ATTATTGTTATTTCTGACAC+AGG 0.564537 6_3:-5325487 Msa0935010:five_prime_UTR
TTCAACCTCCTACGGTATCA+CGG 0.572863 6_3:+5323325 None:intergenic
CTCTTTCCGGTCTTGCTCGG+CGG 0.579534 6_3:-5325203 Msa0935010:CDS
AAAAGTGAATCCTCTAAGCT+CGG 0.595105 6_3:-5323615 Msa0935010:exon
CGTTGTCACCGTGATACCGT+AGG 0.596781 6_3:-5323333 Msa0935010:three_prime_UTR
AATCCAACAAGCTTGGAGGA+GGG 0.600201 6_3:-5325134 Msa0935010:CDS
AAATTTGGGAACAAGTCTCA+TGG 0.606864 6_3:+5323731 None:intergenic
ATGCAATGGAGTTGCTCTGT+AGG 0.608336 6_3:+5325158 None:intergenic
CACCAAAGTTGTAAATTGCA+GGG 0.608423 6_3:-5323285 Msa0935010:three_prime_UTR
TTTATGAGATATGTCGCCAA+AGG 0.631229 6_3:-5323711 Msa0935010:CDS
TGGGGAAGAATATTCCGAGA+AGG 0.635663 6_3:-5323686 Msa0935010:CDS
TGTCACCGTGATACCGTAGG+AGG 0.643418 6_3:-5323330 Msa0935010:three_prime_UTR
GGTTGAAGTGTTGAACACTT+CGG 0.649878 6_3:-5323309 Msa0935010:three_prime_UTR
GTTACAAACACAAAATAACA+AGG 0.663223 6_3:+5323494 None:intergenic
TGTCTCCCATTAGGACACCA+AGG 0.683165 6_3:-5323590 Msa0935010:three_prime_UTR
GATATGTCGCCAAAGGACTG+GGG 0.726161 6_3:-5323704 Msa0935010:CDS
ATTGAACTTGAAGCCCACAG+CGG 0.783498 6_3:+5323784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTTTTTTTTTTTTGT+AGG + chr6_3:5323284-5323303 None:intergenic 10.0%
!!! TTAATTGATAATAAAGTATT+AGG - chr6_3:5323452-5323471 Msa0935010:three_prime_UTR 10.0%
!!! TAATTGATAATAAAGTATTA+GGG - chr6_3:5323453-5323472 Msa0935010:three_prime_UTR 10.0%
!! ATAAATTAATATATATGTAG+CGG + chr6_3:5323893-5323912 None:intergenic 10.0%
!! ATATTAATTTATTTATAGAC+TGG - chr6_3:5323901-5323920 Msa0935010:intron 10.0%
!!! ATGTTTTTTTTTTTTTTTGT+AGG + chr6_3:5323284-5323303 None:intergenic 10.0%
!!! TTAATTGATAATAAAGTATT+AGG - chr6_3:5323452-5323471 Msa0935010:three_prime_UTR 10.0%
!!! TAATTGATAATAAAGTATTA+GGG - chr6_3:5323453-5323472 Msa0935010:three_prime_UTR 10.0%
!! ATAAATTAATATATATGTAG+CGG + chr6_3:5323893-5323912 None:intergenic 10.0%
!! ATATTAATTTATTTATAGAC+TGG - chr6_3:5323901-5323920 Msa0935010:intron 10.0%
!! AAATAATGTTATGTTATACT+AGG + chr6_3:5323261-5323280 None:intergenic 15.0%
!! TATCAATTAAATCAAATCAA+AGG + chr6_3:5323442-5323461 None:intergenic 15.0%
!!! AAGAAAAAACAATTTTATGT+AGG - chr6_3:5324248-5324267 Msa0935010:intron 15.0%
!! AAATAATGTTATGTTATACT+AGG + chr6_3:5323261-5323280 None:intergenic 15.0%
!! TATCAATTAAATCAAATCAA+AGG + chr6_3:5323442-5323461 None:intergenic 15.0%
!!! AAGAAAAAACAATTTTATGT+AGG - chr6_3:5324248-5324267 Msa0935010:intron 15.0%
!!! TCCTATATTTTTTACACTAA+CGG + chr6_3:5323862-5323881 None:intergenic 20.0%
!! TATAAGTGTTATATATGTGT+CGG - chr6_3:5324199-5324218 Msa0935010:intron 20.0%
!! TATGTATGTATATGTATATG+AGG - chr6_3:5324875-5324894 Msa0935010:intron 20.0%
!! ATGTATGTATATGTATATGA+GGG - chr6_3:5324876-5324895 Msa0935010:intron 20.0%
!!! TCCTATATTTTTTACACTAA+CGG + chr6_3:5323862-5323881 None:intergenic 20.0%
!! TATAAGTGTTATATATGTGT+CGG - chr6_3:5324199-5324218 Msa0935010:intron 20.0%
!! TATGTATGTATATGTATATG+AGG - chr6_3:5324875-5324894 Msa0935010:intron 20.0%
!! ATGTATGTATATGTATATGA+GGG - chr6_3:5324876-5324895 Msa0935010:intron 20.0%
! AATGTTATGTTATACTAGGT+CGG + chr6_3:5323257-5323276 None:intergenic 25.0%
!!! ATTATTGTTATTTCTGACAC+AGG - chr6_3:5323302-5323321 Msa0935010:three_prime_UTR 25.0%
! CTGATTGAATTGAATTGAAT+TGG - chr6_3:5323482-5323501 Msa0935010:three_prime_UTR 25.0%
! GATAGTTTGAACATACTTAT+TGG - chr6_3:5323533-5323552 Msa0935010:three_prime_UTR 25.0%
! CTTACATAATTGGAATAACT+GGG + chr6_3:5323748-5323767 None:intergenic 25.0%
! TCTTACATAATTGGAATAAC+TGG + chr6_3:5323749-5323768 None:intergenic 25.0%
! AGTTATTCCAATTATGTAAG+AGG - chr6_3:5323748-5323767 Msa0935010:CDS 25.0%
! TATCTATCCTCTTACATAAT+TGG + chr6_3:5323758-5323777 None:intergenic 25.0%
! TAAGAGGATAGATAGATTAA+TGG - chr6_3:5323764-5323783 Msa0935010:CDS 25.0%
! ACCGTTAGTGTAAAAAATAT+AGG - chr6_3:5323858-5323877 Msa0935010:intron 25.0%
! TTGAGAAATGTTGATCAATT+GGG + chr6_3:5324092-5324111 None:intergenic 25.0%
! TTTGAGAAATGTTGATCAAT+TGG + chr6_3:5324093-5324112 None:intergenic 25.0%
! ATATGTAACTCTTGTATCTA+AGG - chr6_3:5324115-5324134 Msa0935010:intron 25.0%
! AATAAAAAGAAGAAAATCCC+TGG + chr6_3:5324704-5324723 None:intergenic 25.0%
!! AAAACAAAAAAGCACAAGTT+GGG + chr6_3:5324747-5324766 None:intergenic 25.0%
!! AAAAACAAAAAAGCACAAGT+TGG + chr6_3:5324748-5324767 None:intergenic 25.0%
! AAAGAGAAAACATAGAGAAA+TGG + chr6_3:5324930-5324949 None:intergenic 25.0%
!! CATCAAAAGCTTTTATGTTT+TGG - chr6_3:5325136-5325155 Msa0935010:CDS 25.0%
! GTTACAAACACAAAATAACA+AGG + chr6_3:5325298-5325317 None:intergenic 25.0%
!!! GTATTTAGTTACTGCTTTTA+TGG - chr6_3:5325327-5325346 Msa0935010:intron 25.0%
!! TTTTATGGCTTTCTAAATCT+AGG - chr6_3:5325342-5325361 Msa0935010:intron 25.0%
!!! TATAGGTTTCTTTAGAACTT+CGG - chr6_3:5325374-5325393 Msa0935010:intron 25.0%
! AATGTTATGTTATACTAGGT+CGG + chr6_3:5323257-5323276 None:intergenic 25.0%
!!! ATTATTGTTATTTCTGACAC+AGG - chr6_3:5323302-5323321 Msa0935010:three_prime_UTR 25.0%
! CTGATTGAATTGAATTGAAT+TGG - chr6_3:5323482-5323501 Msa0935010:three_prime_UTR 25.0%
! GATAGTTTGAACATACTTAT+TGG - chr6_3:5323533-5323552 Msa0935010:three_prime_UTR 25.0%
! CTTACATAATTGGAATAACT+GGG + chr6_3:5323748-5323767 None:intergenic 25.0%
! TCTTACATAATTGGAATAAC+TGG + chr6_3:5323749-5323768 None:intergenic 25.0%
! AGTTATTCCAATTATGTAAG+AGG - chr6_3:5323748-5323767 Msa0935010:CDS 25.0%
! TATCTATCCTCTTACATAAT+TGG + chr6_3:5323758-5323777 None:intergenic 25.0%
! TAAGAGGATAGATAGATTAA+TGG - chr6_3:5323764-5323783 Msa0935010:CDS 25.0%
! ACCGTTAGTGTAAAAAATAT+AGG - chr6_3:5323858-5323877 Msa0935010:intron 25.0%
! TTGAGAAATGTTGATCAATT+GGG + chr6_3:5324092-5324111 None:intergenic 25.0%
! TTTGAGAAATGTTGATCAAT+TGG + chr6_3:5324093-5324112 None:intergenic 25.0%
! ATATGTAACTCTTGTATCTA+AGG - chr6_3:5324115-5324134 Msa0935010:intron 25.0%
! AATAAAAAGAAGAAAATCCC+TGG + chr6_3:5324704-5324723 None:intergenic 25.0%
!! AAAACAAAAAAGCACAAGTT+GGG + chr6_3:5324747-5324766 None:intergenic 25.0%
!! AAAAACAAAAAAGCACAAGT+TGG + chr6_3:5324748-5324767 None:intergenic 25.0%
! AAAGAGAAAACATAGAGAAA+TGG + chr6_3:5324930-5324949 None:intergenic 25.0%
!! CATCAAAAGCTTTTATGTTT+TGG - chr6_3:5325136-5325155 Msa0935010:CDS 25.0%
! GTTACAAACACAAAATAACA+AGG + chr6_3:5325298-5325317 None:intergenic 25.0%
!!! GTATTTAGTTACTGCTTTTA+TGG - chr6_3:5325327-5325346 Msa0935010:intron 25.0%
!! TTTTATGGCTTTCTAAATCT+AGG - chr6_3:5325342-5325361 Msa0935010:intron 25.0%
!!! TATAGGTTTCTTTAGAACTT+CGG - chr6_3:5325374-5325393 Msa0935010:intron 25.0%
! CTTCTTCAAACGATTGATTT+GGG + chr6_3:5323351-5323370 None:intergenic 30.0%
TTGAATTGAATTGGAAGTTG+TGG - chr6_3:5323491-5323510 Msa0935010:three_prime_UTR 30.0%
ATGAAATACCTGTGATTGTT+GGG + chr6_3:5323691-5323710 None:intergenic 30.0%
AATGAAATACCTGTGATTGT+TGG + chr6_3:5323692-5323711 None:intergenic 30.0%
TAATATATATGTAGCGGTGT+CGG + chr6_3:5323887-5323906 None:intergenic 30.0%
TCATATCAGTCACATAAGAA+AGG + chr6_3:5324431-5324450 None:intergenic 30.0%
!!! CTGACTTTTGTTTTTCTTAG+AGG - chr6_3:5324596-5324615 Msa0935010:intron 30.0%
AAATCCCTGGAGTTAATTAA+TGG + chr6_3:5324691-5324710 None:intergenic 30.0%
TCAATGAGATAACTAAAGCT+CGG + chr6_3:5324904-5324923 None:intergenic 30.0%
! AAGTTCAATGCTTCGTATTT+TGG - chr6_3:5325019-5325038 Msa0935010:intron 30.0%
GCGACATATCTCATAAATTT+GGG + chr6_3:5325075-5325094 None:intergenic 30.0%
!! ATGTTTTGGATAACTATCGT+TGG - chr6_3:5325150-5325169 Msa0935010:CDS 30.0%
!! TGTTTTGGATAACTATCGTT+GGG - chr6_3:5325151-5325170 Msa0935010:CDS 30.0%
! TTAGATGATTTCCTTTTCCT+TGG + chr6_3:5325219-5325238 None:intergenic 30.0%
AATCTAGGAATTGCAGTTAT+AGG - chr6_3:5325357-5325376 Msa0935010:intron 30.0%
! CTTCTTCAAACGATTGATTT+GGG + chr6_3:5323351-5323370 None:intergenic 30.0%
TTGAATTGAATTGGAAGTTG+TGG - chr6_3:5323491-5323510 Msa0935010:three_prime_UTR 30.0%
ATGAAATACCTGTGATTGTT+GGG + chr6_3:5323691-5323710 None:intergenic 30.0%
AATGAAATACCTGTGATTGT+TGG + chr6_3:5323692-5323711 None:intergenic 30.0%
TAATATATATGTAGCGGTGT+CGG + chr6_3:5323887-5323906 None:intergenic 30.0%
TCATATCAGTCACATAAGAA+AGG + chr6_3:5324431-5324450 None:intergenic 30.0%
!!! CTGACTTTTGTTTTTCTTAG+AGG - chr6_3:5324596-5324615 Msa0935010:intron 30.0%
AAATCCCTGGAGTTAATTAA+TGG + chr6_3:5324691-5324710 None:intergenic 30.0%
TCAATGAGATAACTAAAGCT+CGG + chr6_3:5324904-5324923 None:intergenic 30.0%
! AAGTTCAATGCTTCGTATTT+TGG - chr6_3:5325019-5325038 Msa0935010:intron 30.0%
GCGACATATCTCATAAATTT+GGG + chr6_3:5325075-5325094 None:intergenic 30.0%
!! ATGTTTTGGATAACTATCGT+TGG - chr6_3:5325150-5325169 Msa0935010:CDS 30.0%
!! TGTTTTGGATAACTATCGTT+GGG - chr6_3:5325151-5325170 Msa0935010:CDS 30.0%
! TTAGATGATTTCCTTTTCCT+TGG + chr6_3:5325219-5325238 None:intergenic 30.0%
AATCTAGGAATTGCAGTTAT+AGG - chr6_3:5325357-5325376 Msa0935010:intron 30.0%
CCTTCTTCAAACGATTGATT+TGG + chr6_3:5323352-5323371 None:intergenic 35.0%
CCAAATCAATCGTTTGAAGA+AGG - chr6_3:5323349-5323368 Msa0935010:three_prime_UTR 35.0%
ATCAATCGTTTGAAGAAGGT+CGG - chr6_3:5323353-5323372 Msa0935010:three_prime_UTR 35.0%
CTCATTCTCAATTCTTTGCT+TGG - chr6_3:5323712-5323731 Msa0935010:CDS 35.0%
AGAAATGTTGATCAATTGGG+AGG + chr6_3:5324089-5324108 None:intergenic 35.0%
ACTCTTGTATCTAAGGAATG+TGG - chr6_3:5324122-5324141 Msa0935010:intron 35.0%
TAATTCGACACTCTTAAGAC+CGG + chr6_3:5324228-5324247 None:intergenic 35.0%
CAATCAGAGAATGACTATTG+TGG + chr6_3:5324398-5324417 None:intergenic 35.0%
ACACACCATTAATTAACTCC+AGG - chr6_3:5324683-5324702 Msa0935010:intron 35.0%
CACACCATTAATTAACTCCA+GGG - chr6_3:5324684-5324703 Msa0935010:intron 35.0%
! TATTGATTATGTTACCGCTG+TGG - chr6_3:5324991-5325010 Msa0935010:intron 35.0%
ATTGATTATGTTACCGCTGT+GGG - chr6_3:5324992-5325011 Msa0935010:intron 35.0%
AAATTTGGGAACAAGTCTCA+TGG + chr6_3:5325061-5325080 None:intergenic 35.0%
GGCGACATATCTCATAAATT+TGG + chr6_3:5325076-5325095 None:intergenic 35.0%
TTTATGAGATATGTCGCCAA+AGG - chr6_3:5325078-5325097 Msa0935010:intron 35.0%
GATGTATTTCAACTCCTTCT+CGG + chr6_3:5325120-5325139 None:intergenic 35.0%
AAAAGTGAATCCTCTAAGCT+CGG - chr6_3:5325174-5325193 Msa0935010:CDS 35.0%
!! GTTTCTTTAGAACTTCGGTT+TGG - chr6_3:5325379-5325398 Msa0935010:intron 35.0%
TCACCAAAGTTGTAAATTGC+AGG - chr6_3:5325503-5325522 Msa0935010:five_prime_UTR 35.0%
CACCAAAGTTGTAAATTGCA+GGG - chr6_3:5325504-5325523 Msa0935010:five_prime_UTR 35.0%
TACCCTGCAATTTACAACTT+TGG + chr6_3:5325509-5325528 Msa0935020:intron 35.0%
CCTTCTTCAAACGATTGATT+TGG + chr6_3:5323352-5323371 None:intergenic 35.0%
CCAAATCAATCGTTTGAAGA+AGG - chr6_3:5323349-5323368 Msa0935010:three_prime_UTR 35.0%
ATCAATCGTTTGAAGAAGGT+CGG - chr6_3:5323353-5323372 Msa0935010:three_prime_UTR 35.0%
CTCATTCTCAATTCTTTGCT+TGG - chr6_3:5323712-5323731 Msa0935010:CDS 35.0%
AGAAATGTTGATCAATTGGG+AGG + chr6_3:5324089-5324108 None:intergenic 35.0%
ACTCTTGTATCTAAGGAATG+TGG - chr6_3:5324122-5324141 Msa0935010:intron 35.0%
TAATTCGACACTCTTAAGAC+CGG + chr6_3:5324228-5324247 None:intergenic 35.0%
CAATCAGAGAATGACTATTG+TGG + chr6_3:5324398-5324417 None:intergenic 35.0%
ACACACCATTAATTAACTCC+AGG - chr6_3:5324683-5324702 Msa0935010:intron 35.0%
CACACCATTAATTAACTCCA+GGG - chr6_3:5324684-5324703 Msa0935010:intron 35.0%
! TATTGATTATGTTACCGCTG+TGG - chr6_3:5324991-5325010 Msa0935010:intron 35.0%
ATTGATTATGTTACCGCTGT+GGG - chr6_3:5324992-5325011 Msa0935010:intron 35.0%
AAATTTGGGAACAAGTCTCA+TGG + chr6_3:5325061-5325080 None:intergenic 35.0%
GGCGACATATCTCATAAATT+TGG + chr6_3:5325076-5325095 None:intergenic 35.0%
TTTATGAGATATGTCGCCAA+AGG - chr6_3:5325078-5325097 Msa0935010:intron 35.0%
GATGTATTTCAACTCCTTCT+CGG + chr6_3:5325120-5325139 None:intergenic 35.0%
AAAAGTGAATCCTCTAAGCT+CGG - chr6_3:5325174-5325193 Msa0935010:CDS 35.0%
!! GTTTCTTTAGAACTTCGGTT+TGG - chr6_3:5325379-5325398 Msa0935010:intron 35.0%
TCACCAAAGTTGTAAATTGC+AGG - chr6_3:5325503-5325522 Msa0935010:five_prime_UTR 35.0%
CACCAAAGTTGTAAATTGCA+GGG - chr6_3:5325504-5325523 Msa0935010:five_prime_UTR 35.0%
TACCCTGCAATTTACAACTT+TGG + chr6_3:5325509-5325528 Msa0935020:intron 35.0%
GTTTGAAGAAGGTCGGAAAA+GGG - chr6_3:5323360-5323379 Msa0935010:three_prime_UTR 40.0%
! CGAATCGAATCGAGTTTTAC+CGG + chr6_3:5323398-5323417 None:intergenic 40.0%
!! AGCTTGTTGGATTGATGCAA+TGG + chr6_3:5323648-5323667 None:intergenic 40.0%
TTGCATCAATCCAACAAGCT+TGG - chr6_3:5323648-5323667 Msa0935010:CDS 40.0%
TGGCAAATAGCTATGAAGCA+TGG - chr6_3:5323784-5323803 Msa0935010:CDS 40.0%
GTTATATATGTGTCGGACAC+CGG - chr6_3:5324206-5324225 Msa0935010:intron 40.0%
TCGTGTCGGACAACTAAAAT+CGG + chr6_3:5324277-5324296 None:intergenic 40.0%
GACTATTTGCCTATGAAGCA+CGG + chr6_3:5324365-5324384 None:intergenic 40.0%
TAGGCAAATAGTCATTCCAG+TGG - chr6_3:5324372-5324391 Msa0935010:intron 40.0%
ATGATGCATCAGTGAATGAC+TGG + chr6_3:5324953-5324972 None:intergenic 40.0%
AATATTCTTCCCCAGTCCTT+TGG + chr6_3:5325097-5325116 None:intergenic 40.0%
!!! CTTTTCCTTGGTGTCCTAAT+GGG + chr6_3:5325207-5325226 None:intergenic 40.0%
TTCAACACTTCAACCTCCTA+CGG + chr6_3:5325475-5325494 Msa0935020:intron 40.0%
!! GGTTGAAGTGTTGAACACTT+CGG - chr6_3:5325480-5325499 Msa0935010:five_prime_UTR 40.0%
GTTTGAAGAAGGTCGGAAAA+GGG - chr6_3:5323360-5323379 Msa0935010:three_prime_UTR 40.0%
! CGAATCGAATCGAGTTTTAC+CGG + chr6_3:5323398-5323417 None:intergenic 40.0%
!! AGCTTGTTGGATTGATGCAA+TGG + chr6_3:5323648-5323667 None:intergenic 40.0%
TTGCATCAATCCAACAAGCT+TGG - chr6_3:5323648-5323667 Msa0935010:CDS 40.0%
TGGCAAATAGCTATGAAGCA+TGG - chr6_3:5323784-5323803 Msa0935010:CDS 40.0%
GTTATATATGTGTCGGACAC+CGG - chr6_3:5324206-5324225 Msa0935010:intron 40.0%
TCGTGTCGGACAACTAAAAT+CGG + chr6_3:5324277-5324296 None:intergenic 40.0%
GACTATTTGCCTATGAAGCA+CGG + chr6_3:5324365-5324384 None:intergenic 40.0%
TAGGCAAATAGTCATTCCAG+TGG - chr6_3:5324372-5324391 Msa0935010:intron 40.0%
ATGATGCATCAGTGAATGAC+TGG + chr6_3:5324953-5324972 None:intergenic 40.0%
AATATTCTTCCCCAGTCCTT+TGG + chr6_3:5325097-5325116 None:intergenic 40.0%
!!! CTTTTCCTTGGTGTCCTAAT+GGG + chr6_3:5325207-5325226 None:intergenic 40.0%
TTCAACACTTCAACCTCCTA+CGG + chr6_3:5325475-5325494 Msa0935020:intron 40.0%
!! GGTTGAAGTGTTGAACACTT+CGG - chr6_3:5325480-5325499 Msa0935010:five_prime_UTR 40.0%
CGTTTGAAGAAGGTCGGAAA+AGG - chr6_3:5323359-5323378 Msa0935010:three_prime_UTR 45.0%
AAAAGGGTCGTGAATCCAAC+CGG - chr6_3:5323376-5323395 Msa0935010:three_prime_UTR 45.0%
! TCGAATCGAGTTTTACCGGT+TGG + chr6_3:5323394-5323413 None:intergenic 45.0%
!!! GGTTTTGATTCACCTACAGC+AGG - chr6_3:5323554-5323573 Msa0935010:three_prime_UTR 45.0%
CAGGTCACTATGTCTCTTTC+CGG - chr6_3:5323573-5323592 Msa0935010:three_prime_UTR 45.0%
ATGCAATGGAGTTGCTCTGT+AGG + chr6_3:5323634-5323653 None:intergenic 45.0%
CATCAATCCAACAAGCTTGG+AGG - chr6_3:5323651-5323670 Msa0935010:CDS 45.0%
AATCCAACAAGCTTGGAGGA+GGG - chr6_3:5323655-5323674 Msa0935010:CDS 45.0%
ATGAAGCATGGACTCTGACA+CGG - chr6_3:5323796-5323815 Msa0935010:CDS 45.0%
AACACTTATACGAGCCGTGT+CGG + chr6_3:5324189-5324208 None:intergenic 45.0%
AGAATGACTATTGTGGCCAC+TGG + chr6_3:5324391-5324410 None:intergenic 45.0%
ATTGAACTTGAAGCCCACAG+CGG + chr6_3:5325008-5325027 None:intergenic 45.0%
AGATATGTCGCCAAAGGACT+GGG - chr6_3:5325084-5325103 Msa0935010:intron 45.0%
TGGGGAAGAATATTCCGAGA+AGG - chr6_3:5325103-5325122 Msa0935010:intron 45.0%
!!! CCTTTTCCTTGGTGTCCTAA+TGG + chr6_3:5325208-5325227 None:intergenic 45.0%
CCATTAGGACACCAAGGAAA+AGG - chr6_3:5325205-5325224 Msa0935010:CDS 45.0%
TTCAACCTCCTACGGTATCA+CGG + chr6_3:5325467-5325486 None:intergenic 45.0%
CGTTTGAAGAAGGTCGGAAA+AGG - chr6_3:5323359-5323378 Msa0935010:three_prime_UTR 45.0%
AAAAGGGTCGTGAATCCAAC+CGG - chr6_3:5323376-5323395 Msa0935010:three_prime_UTR 45.0%
! TCGAATCGAGTTTTACCGGT+TGG + chr6_3:5323394-5323413 None:intergenic 45.0%
!!! GGTTTTGATTCACCTACAGC+AGG - chr6_3:5323554-5323573 Msa0935010:three_prime_UTR 45.0%
CAGGTCACTATGTCTCTTTC+CGG - chr6_3:5323573-5323592 Msa0935010:three_prime_UTR 45.0%
ATGCAATGGAGTTGCTCTGT+AGG + chr6_3:5323634-5323653 None:intergenic 45.0%
CATCAATCCAACAAGCTTGG+AGG - chr6_3:5323651-5323670 Msa0935010:CDS 45.0%
AATCCAACAAGCTTGGAGGA+GGG - chr6_3:5323655-5323674 Msa0935010:CDS 45.0%
ATGAAGCATGGACTCTGACA+CGG - chr6_3:5323796-5323815 Msa0935010:CDS 45.0%
AACACTTATACGAGCCGTGT+CGG + chr6_3:5324189-5324208 None:intergenic 45.0%
AGAATGACTATTGTGGCCAC+TGG + chr6_3:5324391-5324410 None:intergenic 45.0%
ATTGAACTTGAAGCCCACAG+CGG + chr6_3:5325008-5325027 None:intergenic 45.0%
AGATATGTCGCCAAAGGACT+GGG - chr6_3:5325084-5325103 Msa0935010:intron 45.0%
TGGGGAAGAATATTCCGAGA+AGG - chr6_3:5325103-5325122 Msa0935010:intron 45.0%
!!! CCTTTTCCTTGGTGTCCTAA+TGG + chr6_3:5325208-5325227 None:intergenic 45.0%
CCATTAGGACACCAAGGAAA+AGG - chr6_3:5325205-5325224 Msa0935010:CDS 45.0%
TTCAACCTCCTACGGTATCA+CGG + chr6_3:5325467-5325486 None:intergenic 45.0%
GAGACATAGTGACCTGCTGT+AGG + chr6_3:5323569-5323588 None:intergenic 50.0%
TGTCTCTTTCCGGTCTTGCT+CGG - chr6_3:5323583-5323602 Msa0935010:three_prime_UTR 50.0%
CAATCCAACAAGCTTGGAGG+AGG - chr6_3:5323654-5323673 Msa0935010:CDS 50.0%
ATCCAACAAGCTTGGAGGAG+GGG - chr6_3:5323656-5323675 Msa0935010:CDS 50.0%
TGTCAGTGTCGTGTCATGTC+AGG + chr6_3:5323825-5323844 None:intergenic 50.0%
GACATGACACGACACTGACA+CGG - chr6_3:5323825-5323844 Msa0935010:CDS 50.0%
TTCGTCTCAGTTGTCCGACA+CGG - chr6_3:5324172-5324191 Msa0935010:intron 50.0%
TTGTCCGACACGAGTGTCAT+AGG - chr6_3:5324284-5324303 Msa0935010:intron 50.0%
CACTCCTATGACACTCGTGT+CGG + chr6_3:5324291-5324310 None:intergenic 50.0%
! CACGAGTGTCATAGGAGTGT+CGG - chr6_3:5324292-5324311 Msa0935010:intron 50.0%
TATGAAGCACGGACACCTCT+AGG + chr6_3:5324354-5324373 None:intergenic 50.0%
!! TAGAGGTGTCCGTGCTTCAT+AGG - chr6_3:5324353-5324372 Msa0935010:intron 50.0%
GAGATATGTCGCCAAAGGAC+TGG - chr6_3:5325083-5325102 Msa0935010:intron 50.0%
GATATGTCGCCAAAGGACTG+GGG - chr6_3:5325085-5325104 Msa0935010:intron 50.0%
! AGCTCGGTTTGTCTCCCATT+AGG - chr6_3:5325190-5325209 Msa0935010:CDS 50.0%
TGTCTCCCATTAGGACACCA+AGG - chr6_3:5325199-5325218 Msa0935010:CDS 50.0%
GAGACATAGTGACCTGCTGT+AGG + chr6_3:5323569-5323588 None:intergenic 50.0%
TGTCTCTTTCCGGTCTTGCT+CGG - chr6_3:5323583-5323602 Msa0935010:three_prime_UTR 50.0%
CAATCCAACAAGCTTGGAGG+AGG - chr6_3:5323654-5323673 Msa0935010:CDS 50.0%
ATCCAACAAGCTTGGAGGAG+GGG - chr6_3:5323656-5323675 Msa0935010:CDS 50.0%
TGTCAGTGTCGTGTCATGTC+AGG + chr6_3:5323825-5323844 None:intergenic 50.0%
GACATGACACGACACTGACA+CGG - chr6_3:5323825-5323844 Msa0935010:CDS 50.0%
TTCGTCTCAGTTGTCCGACA+CGG - chr6_3:5324172-5324191 Msa0935010:intron 50.0%
TTGTCCGACACGAGTGTCAT+AGG - chr6_3:5324284-5324303 Msa0935010:intron 50.0%
CACTCCTATGACACTCGTGT+CGG + chr6_3:5324291-5324310 None:intergenic 50.0%
! CACGAGTGTCATAGGAGTGT+CGG - chr6_3:5324292-5324311 Msa0935010:intron 50.0%
TATGAAGCACGGACACCTCT+AGG + chr6_3:5324354-5324373 None:intergenic 50.0%
!! TAGAGGTGTCCGTGCTTCAT+AGG - chr6_3:5324353-5324372 Msa0935010:intron 50.0%
GAGATATGTCGCCAAAGGAC+TGG - chr6_3:5325083-5325102 Msa0935010:intron 50.0%
GATATGTCGCCAAAGGACTG+GGG - chr6_3:5325085-5325104 Msa0935010:intron 50.0%
! AGCTCGGTTTGTCTCCCATT+AGG - chr6_3:5325190-5325209 Msa0935010:CDS 50.0%
TGTCTCCCATTAGGACACCA+AGG - chr6_3:5325199-5325218 Msa0935010:CDS 50.0%
TCCAACAAGCTTGGAGGAGG+GGG - chr6_3:5323657-5323676 Msa0935010:CDS 55.0%
TGCGACACGTCTAGTCCTAG+AGG - chr6_3:5324336-5324355 Msa0935010:intron 55.0%
GGGAGACAAACCGAGCTTAG+AGG + chr6_3:5325187-5325206 None:intergenic 55.0%
CGTTGTCACCGTGATACCGT+AGG - chr6_3:5325456-5325475 Msa0935010:five_prime_UTR 55.0%
TGTCACCGTGATACCGTAGG+AGG - chr6_3:5325459-5325478 Msa0935010:five_prime_UTR 55.0%
TCCAACAAGCTTGGAGGAGG+GGG - chr6_3:5323657-5323676 Msa0935010:CDS 55.0%
TGCGACACGTCTAGTCCTAG+AGG - chr6_3:5324336-5324355 Msa0935010:intron 55.0%
GGGAGACAAACCGAGCTTAG+AGG + chr6_3:5325187-5325206 None:intergenic 55.0%
CGTTGTCACCGTGATACCGT+AGG - chr6_3:5325456-5325475 Msa0935010:five_prime_UTR 55.0%
TGTCACCGTGATACCGTAGG+AGG - chr6_3:5325459-5325478 Msa0935010:five_prime_UTR 55.0%
CTCTTTCCGGTCTTGCTCGG+CGG - chr6_3:5323586-5323605 Msa0935010:three_prime_UTR 60.0%
GAAGATCCGCCGAGCAAGAC+CGG + chr6_3:5323595-5323614 None:intergenic 60.0%
TCCCCCTCCTCCAAGCTTGT+TGG + chr6_3:5323661-5323680 None:intergenic 60.0%
AACAAGCTTGGAGGAGGGGG+AGG - chr6_3:5323660-5323679 Msa0935010:CDS 60.0%
AGGAGGGGCCCAACAATCAC+AGG - chr6_3:5323680-5323699 Msa0935010:CDS 60.0%
CTCTTTCCGGTCTTGCTCGG+CGG - chr6_3:5323586-5323605 Msa0935010:three_prime_UTR 60.0%
GAAGATCCGCCGAGCAAGAC+CGG + chr6_3:5323595-5323614 None:intergenic 60.0%
TCCCCCTCCTCCAAGCTTGT+TGG + chr6_3:5323661-5323680 None:intergenic 60.0%
AACAAGCTTGGAGGAGGGGG+AGG - chr6_3:5323660-5323679 Msa0935010:CDS 60.0%
AGGAGGGGCCCAACAATCAC+AGG - chr6_3:5323680-5323699 Msa0935010:CDS 60.0%
AAGCTTGGAGGAGGGGGAGG+AGG - chr6_3:5323663-5323682 Msa0935010:CDS 65.0%
AGCTTGGAGGAGGGGGAGGA+GGG - chr6_3:5323664-5323683 Msa0935010:CDS 65.0%
TGTCGGACACCGACGCGTGT+CGG - chr6_3:5324309-5324328 Msa0935010:intron 65.0%
TCGCAGTGTCCGACACGCGT+CGG + chr6_3:5324321-5324340 None:intergenic 65.0%
AAGCTTGGAGGAGGGGGAGG+AGG - chr6_3:5323663-5323682 Msa0935010:CDS 65.0%
AGCTTGGAGGAGGGGGAGGA+GGG - chr6_3:5323664-5323683 Msa0935010:CDS 65.0%
TGTCGGACACCGACGCGTGT+CGG - chr6_3:5324309-5324328 Msa0935010:intron 65.0%
TCGCAGTGTCCGACACGCGT+CGG + chr6_3:5324321-5324340 None:intergenic 65.0%
GCTTGGAGGAGGGGGAGGAG+GGG - chr6_3:5323665-5323684 Msa0935010:CDS 70.0%
GCTTGGAGGAGGGGGAGGAG+GGG - chr6_3:5323665-5323684 Msa0935010:CDS 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 5323251 5325560 5323251 ID=Msa0935010;Name=Msa0935010
chr6_3 mRNA 5323251 5325560 5323251 ID=Msa0935010-mRNA-1;Parent=Msa0935010;Name=Msa0935010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=153|1|1|1|0.5|0.33|3|379|115
chr6_3 exon 5323251 5323857 5323251 ID=Msa0935010-mRNA-1:exon:1646;Parent=Msa0935010-mRNA-1
chr6_3 exon 5325110 5325235 5325110 ID=Msa0935010-mRNA-1:exon:1645;Parent=Msa0935010-mRNA-1
chr6_3 exon 5325414 5325560 5325414 ID=Msa0935010-mRNA-1:exon:1644;Parent=Msa0935010-mRNA-1
chr6_3 five_prime_UTR 5325414 5325560 5325414 ID=Msa0935010-mRNA-1:five_prime_utr;Parent=Msa0935010-mRNA-1
chr6_3 five_prime_UTR 5325230 5325235 5325230 ID=Msa0935010-mRNA-1:five_prime_utr;Parent=Msa0935010-mRNA-1
chr6_3 CDS 5325110 5325229 5325110 ID=Msa0935010-mRNA-1:cds;Parent=Msa0935010-mRNA-1
chr6_3 CDS 5323630 5323857 5323630 ID=Msa0935010-mRNA-1:cds;Parent=Msa0935010-mRNA-1
chr6_3 three_prime_UTR 5323251 5323629 5323251 ID=Msa0935010-mRNA-1:three_prime_utr;Parent=Msa0935010-mRNA-1
Gene Sequence

>Msa0935010

ATGTCTCTTTCCGGTCTTGCTCGGCGGATCTTCTCTGCTGCTGCTGCTTCCTACAGAGCAACTCCATTGCATCAATCCAACAAGCTTGGAGGAGGGGGAGGAGGGGCCCAACAATCACAGTCATTCACTGATGCATCATATCACACTGCGAGTGTTTTATTGATTATGTTACCGCTGTGGGCTTCAAGTTCAATGCTTCGTATTTTGGCTATAGACAAACATGTCCATGAGACTTGTTCCCAAATTTATGAGATATGTCGCCAAAGGACTGGGGAAGAATATTCCGAGAAGGAGTTGAAATACATCAAAAGCTTTTATGTTTTGGATAACTATCGTTGGGAAAAGTGA
Protein sequence

>Msa0935010

MSLSGLARRIFSAAAASYRATPLHQSNKLGGGGGGAQQSQSFTDASYHTASVLLIMLPLWASSSMLRILAIDKHVHETCSQIYEICRQRTGEEYSEKELKYIKSFYVLDNYRWEK*