AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0935040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGACATATCTCATAAATT+TGG 0.128373 6_3:+5339884 None:intergenic
AAGTTCAATGCTTCGTATTT+TGG 0.153929 6_3:-5339938 Msa0935040:CDS
AATCTAGGAATTGCAGTTAT+AGG 0.217354 6_3:-5339600 Msa0935040:three_prime_UTR
CCTTCTTCAAACGATTGATT+TGG 0.269274 6_3:+5341638 None:intergenic
CAGGTCACTATGTCTCTTTC+CGG 0.315834 6_3:-5341414 Msa0935040:exon
CTTCTTCAAACGATTGATTT+GGG 0.329224 6_3:+5341639 None:intergenic
GCGACATATCTCATAAATTT+GGG 0.331701 6_3:+5339885 None:intergenic
GATGTATTTCAACTCCTTCT+CGG 0.372367 6_3:+5339840 None:intergenic
TCACCAAAGTTGTAAATTGC+AGG 0.382052 6_3:-5339454 Msa0935040:three_prime_UTR
TACCCTGCAATTTACAACTT+TGG 0.390649 6_3:+5339451 None:intergenic
AATATTCTTCCCCAGTCCTT+TGG 0.392166 6_3:+5339863 None:intergenic
AGCTCGGTTTGTCTCCCATT+AGG 0.411135 6_3:-5339767 Msa0935040:three_prime_UTR
CGTTTGAAGAAGGTCGGAAA+AGG 0.424466 6_3:-5341628 Msa0935040:five_prime_UTR
GAAGATCCGCCGAGCAAGAC+CGG 0.434552 6_3:+5341395 None:intergenic
GAGATATGTCGCCAAAGGAC+TGG 0.434896 6_3:-5339874 Msa0935040:CDS
AGATATGTCGCCAAAGGACT+GGG 0.435686 6_3:-5339873 Msa0935040:CDS
GGGAGACAAACCGAGCTTAG+AGG 0.439522 6_3:+5339773 None:intergenic
CCAAATCAATCGTTTGAAGA+AGG 0.458072 6_3:-5341638 Msa0935040:five_prime_UTR
TCCAACAAGCTTGGAGGAGG+GGG 0.460431 6_3:-5341330 Msa0935040:CDS
CCATTAGGACACCAAGGAAA+AGG 0.476417 6_3:-5339752 Msa0935040:three_prime_UTR
ATGAAATACCTGTGATTGTT+GGG 0.477825 6_3:+5341299 None:intergenic
ATTGATTATGTTACCGCTGT+GGG 0.485009 6_3:-5339965 Msa0935040:CDS
TATAGGTTTCTTTAGAACTT+CGG 0.490184 6_3:-5339583 Msa0935040:three_prime_UTR
GTTTCTTTAGAACTTCGGTT+TGG 0.495359 6_3:-5339578 Msa0935040:three_prime_UTR
AGGAGGGGCCCAACAATCAC+AGG 0.499979 6_3:-5341307 Msa0935040:intron
CAATCCAACAAGCTTGGAGG+AGG 0.510329 6_3:-5341333 Msa0935040:CDS
TATGTTATACTAGGTCGGTT+CGG 0.512627 6_3:+5341738 None:intergenic
AAAAGGGTCGTGAATCCAAC+CGG 0.519654 6_3:-5341611 Msa0935040:intron
TTGCATCAATCCAACAAGCT+TGG 0.523889 6_3:-5341339 Msa0935040:CDS
AATGAAATACCTGTGATTGT+TGG 0.532174 6_3:+5341298 None:intergenic
TGTCTCTTTCCGGTCTTGCT+CGG 0.537525 6_3:-5341404 Msa0935040:CDS
ATCCAACAAGCTTGGAGGAG+GGG 0.542492 6_3:-5341331 Msa0935040:CDS
ATCAATCGTTTGAAGAAGGT+CGG 0.543534 6_3:-5341634 Msa0935040:five_prime_UTR
TATTGATTATGTTACCGCTG+TGG 0.547828 6_3:-5339966 Msa0935040:CDS
GTTTGAAGAAGGTCGGAAAA+GGG 0.549628 6_3:-5341627 Msa0935040:five_prime_UTR
CATCAATCCAACAAGCTTGG+AGG 0.550773 6_3:-5341336 Msa0935040:CDS
AAATAATGTTATGTTATACT+AGG 0.551769 6_3:+5341729 None:intergenic
AATGTTATGTTATACTAGGT+CGG 0.554211 6_3:+5341733 None:intergenic
AGCTTGTTGGATTGATGCAA+TGG 0.556706 6_3:+5341342 None:intergenic
TTCAACACTTCAACCTCCTA+CGG 0.560993 6_3:+5339485 None:intergenic
ATTATTGTTATTTCTGACAC+AGG 0.564537 6_3:-5341685 Msa0935040:five_prime_UTR
TTCAACCTCCTACGGTATCA+CGG 0.572863 6_3:+5339493 None:intergenic
CTCTTTCCGGTCTTGCTCGG+CGG 0.579534 6_3:-5341401 Msa0935040:CDS
AAAAGTGAATCCTCTAAGCT+CGG 0.595105 6_3:-5339783 Msa0935040:exon
CGTTGTCACCGTGATACCGT+AGG 0.596781 6_3:-5339501 Msa0935040:three_prime_UTR
AATCCAACAAGCTTGGAGGA+GGG 0.600201 6_3:-5341332 Msa0935040:CDS
AAATTTGGGAACAAGTCTCA+TGG 0.606864 6_3:+5339899 None:intergenic
ATGCAATGGAGTTGCTCTGT+AGG 0.608336 6_3:+5341356 None:intergenic
CACCAAAGTTGTAAATTGCA+GGG 0.608423 6_3:-5339453 Msa0935040:three_prime_UTR
TTTATGAGATATGTCGCCAA+AGG 0.631229 6_3:-5339879 Msa0935040:CDS
TGGGGAAGAATATTCCGAGA+AGG 0.635663 6_3:-5339854 Msa0935040:CDS
TGTCACCGTGATACCGTAGG+AGG 0.643418 6_3:-5339498 Msa0935040:three_prime_UTR
GGTTGAAGTGTTGAACACTT+CGG 0.649878 6_3:-5339477 Msa0935040:three_prime_UTR
GTTACAAACACAAAATAACA+AGG 0.663223 6_3:+5339662 None:intergenic
TGTCTCCCATTAGGACACCA+AGG 0.683165 6_3:-5339758 Msa0935040:three_prime_UTR
GATATGTCGCCAAAGGACTG+GGG 0.726161 6_3:-5339872 Msa0935040:CDS
ATTGAACTTGAAGCCCACAG+CGG 0.783498 6_3:+5339952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTTTTTTTTTTTTGT+AGG + chr6_3:5339452-5339471 None:intergenic 10.0%
!!! TTAATTGATAATAAAGTATT+AGG - chr6_3:5339620-5339639 Msa0935040:three_prime_UTR 10.0%
!!! TAATTGATAATAAAGTATTA+GGG - chr6_3:5339621-5339640 Msa0935040:three_prime_UTR 10.0%
!! ATAAATTAATATATATGTAG+CGG + chr6_3:5340061-5340080 None:intergenic 10.0%
!! ATATTAATTTATTTATAGAC+TGG - chr6_3:5340069-5340088 Msa0935040:intron 10.0%
!!! ATGTTTTTTTTTTTTTTTGT+AGG + chr6_3:5339452-5339471 None:intergenic 10.0%
!!! TTAATTGATAATAAAGTATT+AGG - chr6_3:5339620-5339639 Msa0935040:three_prime_UTR 10.0%
!!! TAATTGATAATAAAGTATTA+GGG - chr6_3:5339621-5339640 Msa0935040:three_prime_UTR 10.0%
!! ATAAATTAATATATATGTAG+CGG + chr6_3:5340061-5340080 None:intergenic 10.0%
!! ATATTAATTTATTTATAGAC+TGG - chr6_3:5340069-5340088 Msa0935040:intron 10.0%
!! AAATAATGTTATGTTATACT+AGG + chr6_3:5339429-5339448 None:intergenic 15.0%
!! TATCAATTAAATCAAATCAA+AGG + chr6_3:5339610-5339629 None:intergenic 15.0%
!!! AAGAAAAAACAATTTTATGT+AGG - chr6_3:5340416-5340435 Msa0935040:intron 15.0%
!! AAATAATGTTATGTTATACT+AGG + chr6_3:5339429-5339448 None:intergenic 15.0%
!! TATCAATTAAATCAAATCAA+AGG + chr6_3:5339610-5339629 None:intergenic 15.0%
!!! AAGAAAAAACAATTTTATGT+AGG - chr6_3:5340416-5340435 Msa0935040:intron 15.0%
!!! TCCTATATTTTTTACACTAA+CGG + chr6_3:5340030-5340049 None:intergenic 20.0%
!! TATAAGTGTTATATATGTGT+CGG - chr6_3:5340367-5340386 Msa0935040:intron 20.0%
!! TGTATATGTATATGTATATG+AGG - chr6_3:5341067-5341086 Msa0935040:intron 20.0%
!! GTATATGTATATGTATATGA+GGG - chr6_3:5341074-5341093 Msa0935040:intron 20.0%
!!! TCCTATATTTTTTACACTAA+CGG + chr6_3:5340030-5340049 None:intergenic 20.0%
!! TATAAGTGTTATATATGTGT+CGG - chr6_3:5340367-5340386 Msa0935040:intron 20.0%
!! TGTATATGTATATGTATATG+AGG - chr6_3:5341067-5341086 Msa0935040:intron 20.0%
!! GTATATGTATATGTATATGA+GGG - chr6_3:5341074-5341093 Msa0935040:intron 20.0%
! AATGTTATGTTATACTAGGT+CGG + chr6_3:5339425-5339444 None:intergenic 25.0%
!!! ATTATTGTTATTTCTGACAC+AGG - chr6_3:5339470-5339489 Msa0935040:three_prime_UTR 25.0%
! CTGATTGAATTGAATTGAAT+TGG - chr6_3:5339650-5339669 Msa0935040:three_prime_UTR 25.0%
! GATAGTTTGAACATACTTAT+TGG - chr6_3:5339701-5339720 Msa0935040:three_prime_UTR 25.0%
! CTTACATAATTGGAATAACT+GGG + chr6_3:5339916-5339935 None:intergenic 25.0%
! TCTTACATAATTGGAATAAC+TGG + chr6_3:5339917-5339936 None:intergenic 25.0%
! AGTTATTCCAATTATGTAAG+AGG - chr6_3:5339916-5339935 Msa0935040:CDS 25.0%
! TATCTATCCTCTTACATAAT+TGG + chr6_3:5339926-5339945 None:intergenic 25.0%
! TAAGAGGATAGATAGATTAA+TGG - chr6_3:5339932-5339951 Msa0935040:CDS 25.0%
! ACCGTTAGTGTAAAAAATAT+AGG - chr6_3:5340026-5340045 Msa0935040:intron 25.0%
! TTGAGAAATGTTGATCAATT+GGG + chr6_3:5340260-5340279 None:intergenic 25.0%
! TTTGAGAAATGTTGATCAAT+TGG + chr6_3:5340261-5340280 None:intergenic 25.0%
! ATATGTAACTCTTGTATCTA+AGG - chr6_3:5340283-5340302 Msa0935040:intron 25.0%
! AATAAAAAGAAGAAAATCCC+TGG + chr6_3:5340872-5340891 None:intergenic 25.0%
!! AAAACAAAAAAGCACAAGTT+GGG + chr6_3:5340915-5340934 None:intergenic 25.0%
!! AAAAACAAAAAAGCACAAGT+TGG + chr6_3:5340916-5340935 None:intergenic 25.0%
! AAAGAGAAAACATAGAGAAA+TGG + chr6_3:5341128-5341147 None:intergenic 25.0%
!! CATCAAAAGCTTTTATGTTT+TGG - chr6_3:5341334-5341353 Msa0935040:CDS 25.0%
! GTTACAAACACAAAATAACA+AGG + chr6_3:5341496-5341515 None:intergenic 25.0%
!!! GTATTTAGTTACTGCTTTTA+TGG - chr6_3:5341525-5341544 Msa0935040:intron 25.0%
!! TTTTATGGCTTTCTAAATCT+AGG - chr6_3:5341540-5341559 Msa0935040:intron 25.0%
!!! TATAGGTTTCTTTAGAACTT+CGG - chr6_3:5341572-5341591 Msa0935040:intron 25.0%
! AATGTTATGTTATACTAGGT+CGG + chr6_3:5339425-5339444 None:intergenic 25.0%
!!! ATTATTGTTATTTCTGACAC+AGG - chr6_3:5339470-5339489 Msa0935040:three_prime_UTR 25.0%
! CTGATTGAATTGAATTGAAT+TGG - chr6_3:5339650-5339669 Msa0935040:three_prime_UTR 25.0%
! GATAGTTTGAACATACTTAT+TGG - chr6_3:5339701-5339720 Msa0935040:three_prime_UTR 25.0%
! CTTACATAATTGGAATAACT+GGG + chr6_3:5339916-5339935 None:intergenic 25.0%
! TCTTACATAATTGGAATAAC+TGG + chr6_3:5339917-5339936 None:intergenic 25.0%
! AGTTATTCCAATTATGTAAG+AGG - chr6_3:5339916-5339935 Msa0935040:CDS 25.0%
! TATCTATCCTCTTACATAAT+TGG + chr6_3:5339926-5339945 None:intergenic 25.0%
! TAAGAGGATAGATAGATTAA+TGG - chr6_3:5339932-5339951 Msa0935040:CDS 25.0%
! ACCGTTAGTGTAAAAAATAT+AGG - chr6_3:5340026-5340045 Msa0935040:intron 25.0%
! TTGAGAAATGTTGATCAATT+GGG + chr6_3:5340260-5340279 None:intergenic 25.0%
! TTTGAGAAATGTTGATCAAT+TGG + chr6_3:5340261-5340280 None:intergenic 25.0%
! ATATGTAACTCTTGTATCTA+AGG - chr6_3:5340283-5340302 Msa0935040:intron 25.0%
! AATAAAAAGAAGAAAATCCC+TGG + chr6_3:5340872-5340891 None:intergenic 25.0%
!! AAAACAAAAAAGCACAAGTT+GGG + chr6_3:5340915-5340934 None:intergenic 25.0%
!! AAAAACAAAAAAGCACAAGT+TGG + chr6_3:5340916-5340935 None:intergenic 25.0%
! AAAGAGAAAACATAGAGAAA+TGG + chr6_3:5341128-5341147 None:intergenic 25.0%
!! CATCAAAAGCTTTTATGTTT+TGG - chr6_3:5341334-5341353 Msa0935040:CDS 25.0%
! GTTACAAACACAAAATAACA+AGG + chr6_3:5341496-5341515 None:intergenic 25.0%
!!! GTATTTAGTTACTGCTTTTA+TGG - chr6_3:5341525-5341544 Msa0935040:intron 25.0%
!! TTTTATGGCTTTCTAAATCT+AGG - chr6_3:5341540-5341559 Msa0935040:intron 25.0%
!!! TATAGGTTTCTTTAGAACTT+CGG - chr6_3:5341572-5341591 Msa0935040:intron 25.0%
! CTTCTTCAAACGATTGATTT+GGG + chr6_3:5339519-5339538 None:intergenic 30.0%
TTGAATTGAATTGGAAGTTG+TGG - chr6_3:5339659-5339678 Msa0935040:three_prime_UTR 30.0%
ATGAAATACCTGTGATTGTT+GGG + chr6_3:5339859-5339878 None:intergenic 30.0%
AATGAAATACCTGTGATTGT+TGG + chr6_3:5339860-5339879 None:intergenic 30.0%
TAATATATATGTAGCGGTGT+CGG + chr6_3:5340055-5340074 None:intergenic 30.0%
TCATATCAGTCACATAAGAA+AGG + chr6_3:5340599-5340618 None:intergenic 30.0%
!!! CTGACTTTTGTTTTTCTTAG+AGG - chr6_3:5340764-5340783 Msa0935040:intron 30.0%
AAATCCCTGGAGTTAATTAA+TGG + chr6_3:5340859-5340878 None:intergenic 30.0%
TCAATGAGATAACTAAAGCT+CGG + chr6_3:5341102-5341121 None:intergenic 30.0%
! AAGTTCAATGCTTCGTATTT+TGG - chr6_3:5341217-5341236 Msa0935040:intron 30.0%
GCGACATATCTCATAAATTT+GGG + chr6_3:5341273-5341292 None:intergenic 30.0%
!! ATGTTTTGGATAACTATCGT+TGG - chr6_3:5341348-5341367 Msa0935040:CDS 30.0%
!! TGTTTTGGATAACTATCGTT+GGG - chr6_3:5341349-5341368 Msa0935040:CDS 30.0%
! TTAGATGATTTCCTTTTCCT+TGG + chr6_3:5341417-5341436 None:intergenic 30.0%
AATCTAGGAATTGCAGTTAT+AGG - chr6_3:5341555-5341574 Msa0935040:intron 30.0%
! CTTCTTCAAACGATTGATTT+GGG + chr6_3:5339519-5339538 None:intergenic 30.0%
TTGAATTGAATTGGAAGTTG+TGG - chr6_3:5339659-5339678 Msa0935040:three_prime_UTR 30.0%
ATGAAATACCTGTGATTGTT+GGG + chr6_3:5339859-5339878 None:intergenic 30.0%
AATGAAATACCTGTGATTGT+TGG + chr6_3:5339860-5339879 None:intergenic 30.0%
TAATATATATGTAGCGGTGT+CGG + chr6_3:5340055-5340074 None:intergenic 30.0%
TCATATCAGTCACATAAGAA+AGG + chr6_3:5340599-5340618 None:intergenic 30.0%
!!! CTGACTTTTGTTTTTCTTAG+AGG - chr6_3:5340764-5340783 Msa0935040:intron 30.0%
AAATCCCTGGAGTTAATTAA+TGG + chr6_3:5340859-5340878 None:intergenic 30.0%
TCAATGAGATAACTAAAGCT+CGG + chr6_3:5341102-5341121 None:intergenic 30.0%
! AAGTTCAATGCTTCGTATTT+TGG - chr6_3:5341217-5341236 Msa0935040:intron 30.0%
GCGACATATCTCATAAATTT+GGG + chr6_3:5341273-5341292 None:intergenic 30.0%
!! ATGTTTTGGATAACTATCGT+TGG - chr6_3:5341348-5341367 Msa0935040:CDS 30.0%
!! TGTTTTGGATAACTATCGTT+GGG - chr6_3:5341349-5341368 Msa0935040:CDS 30.0%
! TTAGATGATTTCCTTTTCCT+TGG + chr6_3:5341417-5341436 None:intergenic 30.0%
AATCTAGGAATTGCAGTTAT+AGG - chr6_3:5341555-5341574 Msa0935040:intron 30.0%
CCTTCTTCAAACGATTGATT+TGG + chr6_3:5339520-5339539 None:intergenic 35.0%
CCAAATCAATCGTTTGAAGA+AGG - chr6_3:5339517-5339536 Msa0935040:three_prime_UTR 35.0%
ATCAATCGTTTGAAGAAGGT+CGG - chr6_3:5339521-5339540 Msa0935040:three_prime_UTR 35.0%
CTCATTCTCAATTCTTTGCT+TGG - chr6_3:5339880-5339899 Msa0935040:CDS 35.0%
AGAAATGTTGATCAATTGGG+AGG + chr6_3:5340257-5340276 None:intergenic 35.0%
ACTCTTGTATCTAAGGAATG+TGG - chr6_3:5340290-5340309 Msa0935040:intron 35.0%
TAATTCGACACTCTTAAGAC+CGG + chr6_3:5340396-5340415 None:intergenic 35.0%
CAATCAGAGAATGACTATTG+TGG + chr6_3:5340566-5340585 None:intergenic 35.0%
ACACACCATTAATTAACTCC+AGG - chr6_3:5340851-5340870 Msa0935040:intron 35.0%
CACACCATTAATTAACTCCA+GGG - chr6_3:5340852-5340871 Msa0935040:intron 35.0%
! TATTGATTATGTTACCGCTG+TGG - chr6_3:5341189-5341208 Msa0935040:intron 35.0%
ATTGATTATGTTACCGCTGT+GGG - chr6_3:5341190-5341209 Msa0935040:intron 35.0%
AAATTTGGGAACAAGTCTCA+TGG + chr6_3:5341259-5341278 None:intergenic 35.0%
GGCGACATATCTCATAAATT+TGG + chr6_3:5341274-5341293 None:intergenic 35.0%
TTTATGAGATATGTCGCCAA+AGG - chr6_3:5341276-5341295 Msa0935040:intron 35.0%
GATGTATTTCAACTCCTTCT+CGG + chr6_3:5341318-5341337 None:intergenic 35.0%
AAAAGTGAATCCTCTAAGCT+CGG - chr6_3:5341372-5341391 Msa0935040:CDS 35.0%
!! GTTTCTTTAGAACTTCGGTT+TGG - chr6_3:5341577-5341596 Msa0935040:intron 35.0%
TCACCAAAGTTGTAAATTGC+AGG - chr6_3:5341701-5341720 Msa0935040:five_prime_UTR 35.0%
CACCAAAGTTGTAAATTGCA+GGG - chr6_3:5341702-5341721 Msa0935040:five_prime_UTR 35.0%
TACCCTGCAATTTACAACTT+TGG + chr6_3:5341707-5341726 None:intergenic 35.0%
CCTTCTTCAAACGATTGATT+TGG + chr6_3:5339520-5339539 None:intergenic 35.0%
CCAAATCAATCGTTTGAAGA+AGG - chr6_3:5339517-5339536 Msa0935040:three_prime_UTR 35.0%
ATCAATCGTTTGAAGAAGGT+CGG - chr6_3:5339521-5339540 Msa0935040:three_prime_UTR 35.0%
CTCATTCTCAATTCTTTGCT+TGG - chr6_3:5339880-5339899 Msa0935040:CDS 35.0%
AGAAATGTTGATCAATTGGG+AGG + chr6_3:5340257-5340276 None:intergenic 35.0%
ACTCTTGTATCTAAGGAATG+TGG - chr6_3:5340290-5340309 Msa0935040:intron 35.0%
TAATTCGACACTCTTAAGAC+CGG + chr6_3:5340396-5340415 None:intergenic 35.0%
CAATCAGAGAATGACTATTG+TGG + chr6_3:5340566-5340585 None:intergenic 35.0%
ACACACCATTAATTAACTCC+AGG - chr6_3:5340851-5340870 Msa0935040:intron 35.0%
CACACCATTAATTAACTCCA+GGG - chr6_3:5340852-5340871 Msa0935040:intron 35.0%
! TATTGATTATGTTACCGCTG+TGG - chr6_3:5341189-5341208 Msa0935040:intron 35.0%
ATTGATTATGTTACCGCTGT+GGG - chr6_3:5341190-5341209 Msa0935040:intron 35.0%
AAATTTGGGAACAAGTCTCA+TGG + chr6_3:5341259-5341278 None:intergenic 35.0%
GGCGACATATCTCATAAATT+TGG + chr6_3:5341274-5341293 None:intergenic 35.0%
TTTATGAGATATGTCGCCAA+AGG - chr6_3:5341276-5341295 Msa0935040:intron 35.0%
GATGTATTTCAACTCCTTCT+CGG + chr6_3:5341318-5341337 None:intergenic 35.0%
AAAAGTGAATCCTCTAAGCT+CGG - chr6_3:5341372-5341391 Msa0935040:CDS 35.0%
!! GTTTCTTTAGAACTTCGGTT+TGG - chr6_3:5341577-5341596 Msa0935040:intron 35.0%
TCACCAAAGTTGTAAATTGC+AGG - chr6_3:5341701-5341720 Msa0935040:five_prime_UTR 35.0%
CACCAAAGTTGTAAATTGCA+GGG - chr6_3:5341702-5341721 Msa0935040:five_prime_UTR 35.0%
TACCCTGCAATTTACAACTT+TGG + chr6_3:5341707-5341726 None:intergenic 35.0%
GTTTGAAGAAGGTCGGAAAA+GGG - chr6_3:5339528-5339547 Msa0935040:three_prime_UTR 40.0%
! CGAATCGAATCGAGTTTTAC+CGG + chr6_3:5339566-5339585 None:intergenic 40.0%
!! AGCTTGTTGGATTGATGCAA+TGG + chr6_3:5339816-5339835 None:intergenic 40.0%
TTGCATCAATCCAACAAGCT+TGG - chr6_3:5339816-5339835 Msa0935040:CDS 40.0%
TGGCAAATAGCTATGAAGCA+TGG - chr6_3:5339952-5339971 Msa0935040:CDS 40.0%
GTTATATATGTGTCGGACAC+CGG - chr6_3:5340374-5340393 Msa0935040:intron 40.0%
TCGTGTCGGACAACTAAAAT+CGG + chr6_3:5340445-5340464 None:intergenic 40.0%
GACTATTTGCCTATGAAGCA+CGG + chr6_3:5340533-5340552 None:intergenic 40.0%
TAGGCAAATAGTCATTCCAG+TGG - chr6_3:5340540-5340559 Msa0935040:intron 40.0%
ATGATGCATCAGTGAATGAC+TGG + chr6_3:5341151-5341170 None:intergenic 40.0%
AATATTCTTCCCCAGTCCTT+TGG + chr6_3:5341295-5341314 None:intergenic 40.0%
!!! CTTTTCCTTGGTGTCCTAAT+GGG + chr6_3:5341405-5341424 None:intergenic 40.0%
TTCAACACTTCAACCTCCTA+CGG + chr6_3:5341673-5341692 None:intergenic 40.0%
!! GGTTGAAGTGTTGAACACTT+CGG - chr6_3:5341678-5341697 Msa0935040:five_prime_UTR 40.0%
GTTTGAAGAAGGTCGGAAAA+GGG - chr6_3:5339528-5339547 Msa0935040:three_prime_UTR 40.0%
! CGAATCGAATCGAGTTTTAC+CGG + chr6_3:5339566-5339585 None:intergenic 40.0%
!! AGCTTGTTGGATTGATGCAA+TGG + chr6_3:5339816-5339835 None:intergenic 40.0%
TTGCATCAATCCAACAAGCT+TGG - chr6_3:5339816-5339835 Msa0935040:CDS 40.0%
TGGCAAATAGCTATGAAGCA+TGG - chr6_3:5339952-5339971 Msa0935040:CDS 40.0%
GTTATATATGTGTCGGACAC+CGG - chr6_3:5340374-5340393 Msa0935040:intron 40.0%
TCGTGTCGGACAACTAAAAT+CGG + chr6_3:5340445-5340464 None:intergenic 40.0%
GACTATTTGCCTATGAAGCA+CGG + chr6_3:5340533-5340552 None:intergenic 40.0%
TAGGCAAATAGTCATTCCAG+TGG - chr6_3:5340540-5340559 Msa0935040:intron 40.0%
ATGATGCATCAGTGAATGAC+TGG + chr6_3:5341151-5341170 None:intergenic 40.0%
AATATTCTTCCCCAGTCCTT+TGG + chr6_3:5341295-5341314 None:intergenic 40.0%
!!! CTTTTCCTTGGTGTCCTAAT+GGG + chr6_3:5341405-5341424 None:intergenic 40.0%
TTCAACACTTCAACCTCCTA+CGG + chr6_3:5341673-5341692 None:intergenic 40.0%
!! GGTTGAAGTGTTGAACACTT+CGG - chr6_3:5341678-5341697 Msa0935040:five_prime_UTR 40.0%
CGTTTGAAGAAGGTCGGAAA+AGG - chr6_3:5339527-5339546 Msa0935040:three_prime_UTR 45.0%
AAAAGGGTCGTGAATCCAAC+CGG - chr6_3:5339544-5339563 Msa0935040:three_prime_UTR 45.0%
! TCGAATCGAGTTTTACCGGT+TGG + chr6_3:5339562-5339581 None:intergenic 45.0%
!!! GGTTTTGATTCACCTACAGC+AGG - chr6_3:5339722-5339741 Msa0935040:three_prime_UTR 45.0%
CAGGTCACTATGTCTCTTTC+CGG - chr6_3:5339741-5339760 Msa0935040:three_prime_UTR 45.0%
ATGCAATGGAGTTGCTCTGT+AGG + chr6_3:5339802-5339821 None:intergenic 45.0%
CATCAATCCAACAAGCTTGG+AGG - chr6_3:5339819-5339838 Msa0935040:CDS 45.0%
AATCCAACAAGCTTGGAGGA+GGG - chr6_3:5339823-5339842 Msa0935040:CDS 45.0%
AACACTTATACGAGCCGTGT+CGG + chr6_3:5340357-5340376 None:intergenic 45.0%
AGAATGACTATTGTGGCCAC+TGG + chr6_3:5340559-5340578 None:intergenic 45.0%
ATTGAACTTGAAGCCCACAG+CGG + chr6_3:5341206-5341225 None:intergenic 45.0%
AGATATGTCGCCAAAGGACT+GGG - chr6_3:5341282-5341301 Msa0935040:intron 45.0%
TGGGGAAGAATATTCCGAGA+AGG - chr6_3:5341301-5341320 Msa0935040:intron 45.0%
!!! CCTTTTCCTTGGTGTCCTAA+TGG + chr6_3:5341406-5341425 None:intergenic 45.0%
CCATTAGGACACCAAGGAAA+AGG - chr6_3:5341403-5341422 Msa0935040:CDS 45.0%
TTCAACCTCCTACGGTATCA+CGG + chr6_3:5341665-5341684 None:intergenic 45.0%
CGTTTGAAGAAGGTCGGAAA+AGG - chr6_3:5339527-5339546 Msa0935040:three_prime_UTR 45.0%
AAAAGGGTCGTGAATCCAAC+CGG - chr6_3:5339544-5339563 Msa0935040:three_prime_UTR 45.0%
! TCGAATCGAGTTTTACCGGT+TGG + chr6_3:5339562-5339581 None:intergenic 45.0%
!!! GGTTTTGATTCACCTACAGC+AGG - chr6_3:5339722-5339741 Msa0935040:three_prime_UTR 45.0%
CAGGTCACTATGTCTCTTTC+CGG - chr6_3:5339741-5339760 Msa0935040:three_prime_UTR 45.0%
ATGCAATGGAGTTGCTCTGT+AGG + chr6_3:5339802-5339821 None:intergenic 45.0%
CATCAATCCAACAAGCTTGG+AGG - chr6_3:5339819-5339838 Msa0935040:CDS 45.0%
AATCCAACAAGCTTGGAGGA+GGG - chr6_3:5339823-5339842 Msa0935040:CDS 45.0%
AACACTTATACGAGCCGTGT+CGG + chr6_3:5340357-5340376 None:intergenic 45.0%
AGAATGACTATTGTGGCCAC+TGG + chr6_3:5340559-5340578 None:intergenic 45.0%
ATTGAACTTGAAGCCCACAG+CGG + chr6_3:5341206-5341225 None:intergenic 45.0%
AGATATGTCGCCAAAGGACT+GGG - chr6_3:5341282-5341301 Msa0935040:intron 45.0%
TGGGGAAGAATATTCCGAGA+AGG - chr6_3:5341301-5341320 Msa0935040:intron 45.0%
!!! CCTTTTCCTTGGTGTCCTAA+TGG + chr6_3:5341406-5341425 None:intergenic 45.0%
CCATTAGGACACCAAGGAAA+AGG - chr6_3:5341403-5341422 Msa0935040:CDS 45.0%
TTCAACCTCCTACGGTATCA+CGG + chr6_3:5341665-5341684 None:intergenic 45.0%
GAGACATAGTGACCTGCTGT+AGG + chr6_3:5339737-5339756 None:intergenic 50.0%
TGTCTCTTTCCGGTCTTGCT+CGG - chr6_3:5339751-5339770 Msa0935040:three_prime_UTR 50.0%
CAATCCAACAAGCTTGGAGG+AGG - chr6_3:5339822-5339841 Msa0935040:CDS 50.0%
ATCCAACAAGCTTGGAGGAG+GGG - chr6_3:5339824-5339843 Msa0935040:CDS 50.0%
ATGAAGCATGGACTCCGACA+CGG - chr6_3:5339964-5339983 Msa0935040:CDS 50.0%
TGTCAGTGTCGTGTCATGTC+AGG + chr6_3:5339993-5340012 None:intergenic 50.0%
GACATGACACGACACTGACA+CGG - chr6_3:5339993-5340012 Msa0935040:CDS 50.0%
TTCGTCTCAGTTGTCCGACA+CGG - chr6_3:5340340-5340359 Msa0935040:intron 50.0%
TTGTCCGACACGAGTGTCAT+AGG - chr6_3:5340452-5340471 Msa0935040:intron 50.0%
CACTCCTATGACACTCGTGT+CGG + chr6_3:5340459-5340478 None:intergenic 50.0%
! CACGAGTGTCATAGGAGTGT+CGG - chr6_3:5340460-5340479 Msa0935040:intron 50.0%
TATGAAGCACGGACACCACT+AGG + chr6_3:5340522-5340541 None:intergenic 50.0%
TAGTGGTGTCCGTGCTTCAT+AGG - chr6_3:5340521-5340540 Msa0935040:intron 50.0%
GAGATATGTCGCCAAAGGAC+TGG - chr6_3:5341281-5341300 Msa0935040:intron 50.0%
GATATGTCGCCAAAGGACTG+GGG - chr6_3:5341283-5341302 Msa0935040:intron 50.0%
! AGCTCGGTTTGTCTCCCATT+AGG - chr6_3:5341388-5341407 Msa0935040:CDS 50.0%
TGTCTCCCATTAGGACACCA+AGG - chr6_3:5341397-5341416 Msa0935040:CDS 50.0%
GAGACATAGTGACCTGCTGT+AGG + chr6_3:5339737-5339756 None:intergenic 50.0%
TGTCTCTTTCCGGTCTTGCT+CGG - chr6_3:5339751-5339770 Msa0935040:three_prime_UTR 50.0%
CAATCCAACAAGCTTGGAGG+AGG - chr6_3:5339822-5339841 Msa0935040:CDS 50.0%
ATCCAACAAGCTTGGAGGAG+GGG - chr6_3:5339824-5339843 Msa0935040:CDS 50.0%
ATGAAGCATGGACTCCGACA+CGG - chr6_3:5339964-5339983 Msa0935040:CDS 50.0%
TGTCAGTGTCGTGTCATGTC+AGG + chr6_3:5339993-5340012 None:intergenic 50.0%
GACATGACACGACACTGACA+CGG - chr6_3:5339993-5340012 Msa0935040:CDS 50.0%
TTCGTCTCAGTTGTCCGACA+CGG - chr6_3:5340340-5340359 Msa0935040:intron 50.0%
TTGTCCGACACGAGTGTCAT+AGG - chr6_3:5340452-5340471 Msa0935040:intron 50.0%
CACTCCTATGACACTCGTGT+CGG + chr6_3:5340459-5340478 None:intergenic 50.0%
! CACGAGTGTCATAGGAGTGT+CGG - chr6_3:5340460-5340479 Msa0935040:intron 50.0%
TATGAAGCACGGACACCACT+AGG + chr6_3:5340522-5340541 None:intergenic 50.0%
TAGTGGTGTCCGTGCTTCAT+AGG - chr6_3:5340521-5340540 Msa0935040:intron 50.0%
GAGATATGTCGCCAAAGGAC+TGG - chr6_3:5341281-5341300 Msa0935040:intron 50.0%
GATATGTCGCCAAAGGACTG+GGG - chr6_3:5341283-5341302 Msa0935040:intron 50.0%
! AGCTCGGTTTGTCTCCCATT+AGG - chr6_3:5341388-5341407 Msa0935040:CDS 50.0%
TGTCTCCCATTAGGACACCA+AGG - chr6_3:5341397-5341416 Msa0935040:CDS 50.0%
TCCAACAAGCTTGGAGGAGG+GGG - chr6_3:5339825-5339844 Msa0935040:CDS 55.0%
GTCATGTCAGGTGTCCGTGT+CGG + chr6_3:5339981-5340000 None:intergenic 55.0%
TGCGACACGTCTAGTCCTAG+TGG - chr6_3:5340504-5340523 Msa0935040:intron 55.0%
GGGAGACAAACCGAGCTTAG+AGG + chr6_3:5341385-5341404 None:intergenic 55.0%
CGTTGTCACCGTGATACCGT+AGG - chr6_3:5341654-5341673 Msa0935040:five_prime_UTR 55.0%
TGTCACCGTGATACCGTAGG+AGG - chr6_3:5341657-5341676 Msa0935040:five_prime_UTR 55.0%
TCCAACAAGCTTGGAGGAGG+GGG - chr6_3:5339825-5339844 Msa0935040:CDS 55.0%
GTCATGTCAGGTGTCCGTGT+CGG + chr6_3:5339981-5340000 None:intergenic 55.0%
TGCGACACGTCTAGTCCTAG+TGG - chr6_3:5340504-5340523 Msa0935040:intron 55.0%
GGGAGACAAACCGAGCTTAG+AGG + chr6_3:5341385-5341404 None:intergenic 55.0%
CGTTGTCACCGTGATACCGT+AGG - chr6_3:5341654-5341673 Msa0935040:five_prime_UTR 55.0%
TGTCACCGTGATACCGTAGG+AGG - chr6_3:5341657-5341676 Msa0935040:five_prime_UTR 55.0%
CTCTTTCCGGTCTTGCTCGG+CGG - chr6_3:5339754-5339773 Msa0935040:three_prime_UTR 60.0%
GAAGATCCGCCGAGCAAGAC+CGG + chr6_3:5339763-5339782 None:intergenic 60.0%
TCCCCCTCCTCCAAGCTTGT+TGG + chr6_3:5339829-5339848 None:intergenic 60.0%
AACAAGCTTGGAGGAGGGGG+AGG - chr6_3:5339828-5339847 Msa0935040:CDS 60.0%
AGGAGGGGCCCAACAATCAC+AGG - chr6_3:5339848-5339867 Msa0935040:CDS 60.0%
CTCTTTCCGGTCTTGCTCGG+CGG - chr6_3:5339754-5339773 Msa0935040:three_prime_UTR 60.0%
GAAGATCCGCCGAGCAAGAC+CGG + chr6_3:5339763-5339782 None:intergenic 60.0%
TCCCCCTCCTCCAAGCTTGT+TGG + chr6_3:5339829-5339848 None:intergenic 60.0%
AACAAGCTTGGAGGAGGGGG+AGG - chr6_3:5339828-5339847 Msa0935040:CDS 60.0%
AGGAGGGGCCCAACAATCAC+AGG - chr6_3:5339848-5339867 Msa0935040:CDS 60.0%
AAGCTTGGAGGAGGGGGAGG+AGG - chr6_3:5339831-5339850 Msa0935040:CDS 65.0%
AGCTTGGAGGAGGGGGAGGA+GGG - chr6_3:5339832-5339851 Msa0935040:CDS 65.0%
TGTCGGACACCGACGCGTGT+CGG - chr6_3:5340477-5340496 Msa0935040:intron 65.0%
TCGCAGTGTCCGACACGCGT+CGG + chr6_3:5340489-5340508 None:intergenic 65.0%
AAGCTTGGAGGAGGGGGAGG+AGG - chr6_3:5339831-5339850 Msa0935040:CDS 65.0%
AGCTTGGAGGAGGGGGAGGA+GGG - chr6_3:5339832-5339851 Msa0935040:CDS 65.0%
TGTCGGACACCGACGCGTGT+CGG - chr6_3:5340477-5340496 Msa0935040:intron 65.0%
TCGCAGTGTCCGACACGCGT+CGG + chr6_3:5340489-5340508 None:intergenic 65.0%
GCTTGGAGGAGGGGGAGGAG+GGG - chr6_3:5339833-5339852 Msa0935040:CDS 70.0%
GCTTGGAGGAGGGGGAGGAG+GGG - chr6_3:5339833-5339852 Msa0935040:CDS 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 5339419 5341758 5339419 ID=Msa0935040;Name=Msa0935040
chr6_3 mRNA 5339419 5341758 5339419 ID=Msa0935040-mRNA-1;Parent=Msa0935040;Name=Msa0935040-mRNA-1;_AED=0.00;_eAED=0.00;_QI=153|1|1|1|0.5|0.33|3|379|115
chr6_3 exon 5339419 5340025 5339419 ID=Msa0935040-mRNA-1:exon:1649;Parent=Msa0935040-mRNA-1
chr6_3 exon 5341308 5341433 5341308 ID=Msa0935040-mRNA-1:exon:1648;Parent=Msa0935040-mRNA-1
chr6_3 exon 5341612 5341758 5341612 ID=Msa0935040-mRNA-1:exon:1647;Parent=Msa0935040-mRNA-1
chr6_3 five_prime_UTR 5341612 5341758 5341612 ID=Msa0935040-mRNA-1:five_prime_utr;Parent=Msa0935040-mRNA-1
chr6_3 five_prime_UTR 5341428 5341433 5341428 ID=Msa0935040-mRNA-1:five_prime_utr;Parent=Msa0935040-mRNA-1
chr6_3 CDS 5341308 5341427 5341308 ID=Msa0935040-mRNA-1:cds;Parent=Msa0935040-mRNA-1
chr6_3 CDS 5339798 5340025 5339798 ID=Msa0935040-mRNA-1:cds;Parent=Msa0935040-mRNA-1
chr6_3 three_prime_UTR 5339419 5339797 5339419 ID=Msa0935040-mRNA-1:three_prime_utr;Parent=Msa0935040-mRNA-1
Gene Sequence

>Msa0935040

ATGTCTCTTTCCGGTCTTGCTCGGCGGATCTTCTCTGCTGCTGCTGCTTCCTACAGAGCAACTCCATTGCATCAATCCAACAAGCTTGGAGGAGGGGGAGGAGGGGCCCAACAATCACAGTCATTCACTGATGCATCATATCACACTGCGAGTGTTTTATTGATTATGTTACCGCTGTGGGCTTCAAGTTCAATGCTTCGTATTTTGGCTATAGACAAACATGTCCATGAGACTTGTTCCCAAATTTATGAGATATGTCGCCAAAGGACTGGGGAAGAATATTCCGAGAAGGAGTTGAAATACATCAAAAGCTTTTATGTTTTGGATAACTATCGTTGGGAAAAGTGA
Protein sequence

>Msa0935040

MSLSGLARRIFSAAAASYRATPLHQSNKLGGGGGGAQQSQSFTDASYHTASVLLIMLPLWASSSMLRILAIDKHVHETCSQIYEICRQRTGEEYSEKELKYIKSFYVLDNYRWEK*