AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0944220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0944220 MtrunA17_Chr6g0461111 98.889 270 3 0 1 270 496 765 0.0 567
Msa0944220 MtrunA17_Chr3g0118901 91.111 270 24 0 1 270 544 813 0.0 525
Msa0944220 MtrunA17_Chr7g0253781 91.011 267 24 0 1 267 496 762 0.0 521
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0944220 AT5G17920.2 89.630 270 28 0 1 270 496 765 0.0 525
Msa0944220 AT5G17920.1 89.630 270 28 0 1 270 496 765 0.0 525
Msa0944220 AT3G03780.1 89.630 270 28 0 1 270 496 765 0.0 521
Msa0944220 AT3G03780.3 89.630 270 28 0 1 270 496 765 0.0 521
Msa0944220 AT3G03780.2 89.630 270 28 0 1 270 496 765 0.0 521
Msa0944220 AT5G20980.2 87.970 266 32 0 1 266 544 809 7.50e-176 505
Msa0944220 AT5G20980.1 87.970 266 32 0 1 266 544 809 7.50e-176 505

Find 92 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTTGCCTTCACTGTTAA+TGG 0.131956 6_3:+21001994 Msa0944220:CDS
ACTGGGCTGTCCATTCTTTC+AGG 0.146160 6_3:+21002453 Msa0944220:CDS
CAGAAAATTACAAAATGTTT+TGG 0.229530 6_3:-21003268 None:intergenic
TTGAAGTTCCTCTCATCTTC+TGG 0.243240 6_3:-21003389 None:intergenic
CTTCAGTTGGTGGTATTCTT+GGG 0.266305 6_3:-21002892 None:intergenic
TCAAAGCAGCCTCATCAATT+TGG 0.273412 6_3:-21002385 None:intergenic
TCTTCAGTTGGTGGTATTCT+TGG 0.276364 6_3:-21002893 None:intergenic
TACTTTGGTGAGCAGTTATC+AGG 0.280762 6_3:+21001973 Msa0944220:CDS
CCAATGAAGGGAATGCTTAC+TGG 0.290098 6_3:+21002135 Msa0944220:CDS
TTCATTGCAGAGAAATGATA+TGG 0.303786 6_3:+21001945 Msa0944220:intron
TCAACCATGGCTGTGAGAGC+TGG 0.310243 6_3:-21003019 None:intergenic
CATTCCCTTCATTGGGCGTT+TGG 0.334822 6_3:-21002127 None:intergenic
CTCTCAGCAATTTCTTCAGT+TGG 0.348497 6_3:-21002905 None:intergenic
CCAGTAAGCATTCCCTTCAT+TGG 0.357787 6_3:-21002135 None:intergenic
AATGGATGGGTGCAATCTTA+TGG 0.358906 6_3:+21002012 Msa0944220:CDS
AATAACTGTGTTGAATATCT+AGG 0.360248 6_3:+21003180 Msa0944220:three_prime_UTR
CTAAGGCAATGACCGTCTTC+TGG 0.362564 6_3:+21002082 Msa0944220:CDS
TATGATTATCTTCAACTGTA+TGG 0.372887 6_3:-21003417 None:intergenic
GCTTGCAAGTGGAAAGTAAA+TGG 0.376739 6_3:+21003066 Msa0944220:exon
AGAACCATTGTGTGATTACT+TGG 0.382276 6_3:-21003110 None:intergenic
GACCGTCTTCTGGTCATCTA+TGG 0.387644 6_3:+21002092 Msa0944220:CDS
CAGTAAGCATTCCCTTCATT+GGG 0.413257 6_3:-21002134 None:intergenic
TCAGATTGCTTTGGCTATCA+AGG 0.421082 6_3:+21002322 Msa0944220:CDS
GCCCTGTCACCATTCTCAAT+TGG 0.428135 6_3:+21002157 Msa0944220:CDS
TGCCTTCACTGTTAATGGAT+GGG 0.430204 6_3:+21001999 Msa0944220:CDS
TTCCACTTGCAAGCTGGTTG+CGG 0.432829 6_3:-21003057 None:intergenic
GTTAAGTATGGTGCTGGAAT+TGG 0.435441 6_3:+21002845 Msa0944220:CDS
TCAGCAATTTCTTCAGTTGG+TGG 0.438629 6_3:-21002902 None:intergenic
ATCTGAACAATCCCACTACT+TGG 0.441486 6_3:+21002430 Msa0944220:CDS
TTGCCTTCACTGTTAATGGA+TGG 0.442821 6_3:+21001998 Msa0944220:CDS
AACAATCCCACTACTTGGAC+TGG 0.454548 6_3:+21002435 Msa0944220:CDS
CAACCATGGCTGTGAGAGCT+GGG 0.457959 6_3:-21003018 None:intergenic
GATTGTTCAGATTTCCTCAA+TGG 0.466611 6_3:-21002419 None:intergenic
CATTGGTGATCCTGAAAGAA+TGG 0.469994 6_3:-21002463 None:intergenic
CAGAAGACGGTCATTGCCTT+AGG 0.471991 6_3:-21002081 None:intergenic
GTGAAGCCACCAATCATCTA+TGG 0.474426 6_3:+21002045 Msa0944220:CDS
TTCCGCGATGGAGTTAAGTA+TGG 0.474863 6_3:+21002833 Msa0944220:CDS
AATGATATGGTTGAGTACTT+TGG 0.476014 6_3:+21001958 Msa0944220:exon
GACTTGCTATCAGATTGCTT+TGG 0.476061 6_3:+21002313 Msa0944220:CDS
GAGTGAATGTCATAAACTCC+GGG 0.485839 6_3:-21002869 None:intergenic
TGAGAGAAGGGTTGCCATTG+AGG 0.496632 6_3:+21002405 Msa0944220:CDS
TTCAGTTGGTGGTATTCTTG+GGG 0.497632 6_3:-21002891 None:intergenic
CGTAGAATTTATACCTAGGT+TGG 0.501132 6_3:-21002197 None:intergenic
GATCACCAATGTTGGTGTGC+AGG 0.501767 6_3:+21002475 Msa0944220:CDS
AATTTGGATAACAGTAATAC+CGG 0.501975 6_3:-21002369 None:intergenic
CTCACATCACCATAGATGAT+TGG 0.510249 6_3:-21002054 None:intergenic
GTTCTGATGAAAAGCTCCTA+AGG 0.512466 6_3:+21002807 Msa0944220:CDS
GATGAGGCTGCTTTGAGAGA+AGG 0.517170 6_3:+21002392 Msa0944220:CDS
CATGACCAAACGCCCAATGA+AGG 0.519465 6_3:+21002122 Msa0944220:CDS
TTTACTTTCCACTTGCAAGC+TGG 0.520445 6_3:-21003063 None:intergenic
ATGAGGCTGCTTTGAGAGAA+GGG 0.524459 6_3:+21002393 Msa0944220:CDS
GACCAATTGAGAATGGTGAC+AGG 0.529832 6_3:-21002159 None:intergenic
TGGTGATGTGAGCCGTCCTA+AGG 0.546492 6_3:+21002065 Msa0944220:CDS
GATGGAGTTAAGTATGGTGC+TGG 0.556844 6_3:+21002839 Msa0944220:CDS
AATCACACAATGGTTCTGCT+TGG 0.558655 6_3:+21003116 Msa0944220:three_prime_UTR
TCTTTCAGGATCACCAATGT+TGG 0.558869 6_3:+21002467 Msa0944220:CDS
AGCTCTCACAGCCATGGTTG+AGG 0.560165 6_3:+21003021 Msa0944220:CDS
CATCCACTCAATCATTGACA+TGG 0.567620 6_3:+21002754 Msa0944220:CDS
GAGAGTAGCACATGTGGGTG+TGG 0.568344 6_3:-21002718 None:intergenic
GGAGTGAATGTCATAAACTC+CGG 0.569880 6_3:-21002870 None:intergenic
TTGTTCGTAATGACCAACCT+AGG 0.571289 6_3:+21002184 Msa0944220:CDS
AGTATCCTGCACACCAACAT+TGG 0.573572 6_3:-21002480 None:intergenic
TACTGTTATCCAAATTGATG+AGG 0.574165 6_3:+21002376 Msa0944220:CDS
TATGGTGCTGGAATTGGCCC+CGG 0.574166 6_3:+21002851 Msa0944220:CDS
AACAAAGGACCAATTGAGAA+TGG 0.575012 6_3:-21002166 None:intergenic
ACATCACCATAGATGATTGG+TGG 0.578253 6_3:-21002051 None:intergenic
ATGACCAAACGCCCAATGAA+GGG 0.580031 6_3:+21002123 Msa0944220:CDS
TTGTGGGTCAATCCTGATTG+TGG 0.580379 6_3:+21002965 Msa0944220:CDS
ATCAGAACGAGAATTCTCAA+TGG 0.580471 6_3:-21002792 None:intergenic
CACCCATCCATTAACAGTGA+AGG 0.585556 6_3:-21002001 None:intergenic
ATCCGCAACCAGCTTGCAAG+TGG 0.598138 6_3:+21003055 Msa0944220:CDS
GACAGCCCAGTCCAAGTAGT+GGG 0.602135 6_3:-21002441 None:intergenic
TTCTGATGAAAAGCTCCTAA+GGG 0.605088 6_3:+21002808 Msa0944220:CDS
TGTGAGAGCTGGGTTCACCT+CGG 0.611312 6_3:-21003008 None:intergenic
AGCAATCTGATAGCAAGTCT+CGG 0.611346 6_3:-21002309 None:intergenic
ACAATCCCACTACTTGGACT+GGG 0.617936 6_3:+21002436 Msa0944220:CDS
GGACAGCCCAGTCCAAGTAG+TGG 0.620634 6_3:-21002442 None:intergenic
GAACCCAGCTCTCACAGCCA+TGG 0.622406 6_3:+21003015 Msa0944220:CDS
CATCCATGTCAATGATTGAG+TGG 0.624571 6_3:-21002757 None:intergenic
AGGTTGGTCATTACGAACAA+AGG 0.635061 6_3:-21002181 None:intergenic
AGACGGTCATTGCCTTAGGA+CGG 0.640342 6_3:-21002077 None:intergenic
TATTTAGTCCAGAAGATGAG+AGG 0.641391 6_3:+21003381 Msa0944220:three_prime_UTR
ACCAATTGAGAATGGTGACA+GGG 0.646821 6_3:-21002158 None:intergenic
AGCCATAGATGACCAGAAGA+CGG 0.665553 6_3:-21002094 None:intergenic
TGGTGTGCAGGATACTACTC+AGG 0.675205 6_3:+21002487 Msa0944220:CDS
TGAAGTGAGAGTAGCACATG+TGG 0.675505 6_3:-21002724 None:intergenic
GAATCCAAGTAATCACACAA+TGG 0.703039 6_3:+21003106 Msa0944220:three_prime_UTR
CAACTGAAGAAATTGCTGAG+AGG 0.727912 6_3:+21002906 Msa0944220:CDS
GAAGTGAGAGTAGCACATGT+GGG 0.737320 6_3:-21002723 None:intergenic
CACCATACTTAACTCCATCG+CGG 0.739326 6_3:-21002835 None:intergenic
AGTGAATGTCATAAACTCCG+GGG 0.741156 6_3:-21002868 None:intergenic
CAAAACTCGCAAGTACACCG+AGG 0.785977 6_3:+21002991 Msa0944220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTATTGTTATTTCTAACA+TGG + chr6_3:21002266-21002285 Msa0944220:intron 20.0%
!!! AATGCAAATTTTTCGTTTTT+CGG + chr6_3:21003148-21003167 Msa0944220:three_prime_UTR 20.0%
!!! CAGAAAATTACAAAATGTTT+TGG - chr6_3:21003271-21003290 None:intergenic 20.0%
!!! AAACATTTTGTAATTTTCTG+TGG + chr6_3:21003271-21003290 Msa0944220:three_prime_UTR 20.0%
!!! CTTTATTGTTATTTCTAACA+TGG + chr6_3:21002266-21002285 Msa0944220:intron 20.0%
!!! AATGCAAATTTTTCGTTTTT+CGG + chr6_3:21003148-21003167 Msa0944220:three_prime_UTR 20.0%
!!! CAGAAAATTACAAAATGTTT+TGG - chr6_3:21003271-21003290 None:intergenic 20.0%
!!! AAACATTTTGTAATTTTCTG+TGG + chr6_3:21003271-21003290 Msa0944220:three_prime_UTR 20.0%
! GAAAAGTGAAAACAATTAGT+TGG - chr6_3:21001928-21001947 None:intergenic 25.0%
! AATTTGGATAACAGTAATAC+CGG - chr6_3:21002372-21002391 None:intergenic 25.0%
! AATAACTGTGTTGAATATCT+AGG + chr6_3:21003180-21003199 Msa0944220:three_prime_UTR 25.0%
! TATGATTATCTTCAACTGTA+TGG - chr6_3:21003420-21003439 None:intergenic 25.0%
! GAAAAGTGAAAACAATTAGT+TGG - chr6_3:21001928-21001947 None:intergenic 25.0%
! AATTTGGATAACAGTAATAC+CGG - chr6_3:21002372-21002391 None:intergenic 25.0%
! AATAACTGTGTTGAATATCT+AGG + chr6_3:21003180-21003199 Msa0944220:three_prime_UTR 25.0%
! TATGATTATCTTCAACTGTA+TGG - chr6_3:21003420-21003439 None:intergenic 25.0%
! CAATTAGTTGGAAGTACAAT+CGG - chr6_3:21001916-21001935 None:intergenic 30.0%
TTCATTGCAGAGAAATGATA+TGG + chr6_3:21001945-21001964 Msa0944220:intron 30.0%
AATGATATGGTTGAGTACTT+TGG + chr6_3:21001958-21001977 Msa0944220:exon 30.0%
TACTGTTATCCAAATTGATG+AGG + chr6_3:21002376-21002395 Msa0944220:CDS 30.0%
TGTATTACAAAGCATAGACA+TGG - chr6_3:21002634-21002653 None:intergenic 30.0%
CAAGATATGTAAGTTAGAAG+GGG - chr6_3:21002677-21002696 None:intergenic 30.0%
CCAAGATATGTAAGTTAGAA+GGG - chr6_3:21002678-21002697 None:intergenic 30.0%
TCCAAGATATGTAAGTTAGA+AGG - chr6_3:21002679-21002698 None:intergenic 30.0%
AAGCAAAAAAGATGTGTTGT+GGG - chr6_3:21003240-21003259 None:intergenic 30.0%
AAAGCAAAAAAGATGTGTTG+TGG - chr6_3:21003241-21003260 None:intergenic 30.0%
! CAATTAGTTGGAAGTACAAT+CGG - chr6_3:21001916-21001935 None:intergenic 30.0%
TTCATTGCAGAGAAATGATA+TGG + chr6_3:21001945-21001964 Msa0944220:intron 30.0%
AATGATATGGTTGAGTACTT+TGG + chr6_3:21001958-21001977 Msa0944220:exon 30.0%
TACTGTTATCCAAATTGATG+AGG + chr6_3:21002376-21002395 Msa0944220:CDS 30.0%
TGTATTACAAAGCATAGACA+TGG - chr6_3:21002634-21002653 None:intergenic 30.0%
CAAGATATGTAAGTTAGAAG+GGG - chr6_3:21002677-21002696 None:intergenic 30.0%
CCAAGATATGTAAGTTAGAA+GGG - chr6_3:21002678-21002697 None:intergenic 30.0%
TCCAAGATATGTAAGTTAGA+AGG - chr6_3:21002679-21002698 None:intergenic 30.0%
AAGCAAAAAAGATGTGTTGT+GGG - chr6_3:21003240-21003259 None:intergenic 30.0%
AAAGCAAAAAAGATGTGTTG+TGG - chr6_3:21003241-21003260 None:intergenic 30.0%
TGAATATGAGAACTAACCTC+AGG - chr6_3:21001838-21001857 None:intergenic 35.0%
AACAAAGGACCAATTGAGAA+TGG - chr6_3:21002169-21002188 None:intergenic 35.0%
CGTAGAATTTATACCTAGGT+TGG - chr6_3:21002200-21002219 None:intergenic 35.0%
GAGTCGTAGAATTTATACCT+AGG - chr6_3:21002204-21002223 None:intergenic 35.0%
TTCTACGACTCATCTTAATG+TGG + chr6_3:21002213-21002232 Msa0944220:intron 35.0%
GATTGTTCAGATTTCCTCAA+TGG - chr6_3:21002422-21002441 None:intergenic 35.0%
CCCTTCTAACTTACATATCT+TGG + chr6_3:21002675-21002694 Msa0944220:intron 35.0%
ATCAGAACGAGAATTCTCAA+TGG - chr6_3:21002795-21002814 None:intergenic 35.0%
TTCTGATGAAAAGCTCCTAA+GGG + chr6_3:21002808-21002827 Msa0944220:CDS 35.0%
! TGCTTGAGAAAAACGTTTTG+TGG + chr6_3:21002948-21002967 Msa0944220:CDS 35.0%
! GCTTGAGAAAAACGTTTTGT+GGG + chr6_3:21002949-21002968 Msa0944220:CDS 35.0%
GAATCCAAGTAATCACACAA+TGG + chr6_3:21003106-21003125 Msa0944220:three_prime_UTR 35.0%
AGAACCATTGTGTGATTACT+TGG - chr6_3:21003113-21003132 None:intergenic 35.0%
! TTAGTATGCTCTTTTGAGCA+AGG + chr6_3:21003206-21003225 Msa0944220:three_prime_UTR 35.0%
! TAGTATGCTCTTTTGAGCAA+GGG + chr6_3:21003207-21003226 Msa0944220:three_prime_UTR 35.0%
AGCAAAAAAGATGTGTTGTG+GGG - chr6_3:21003239-21003258 None:intergenic 35.0%
TATTTAGTCCAGAAGATGAG+AGG + chr6_3:21003381-21003400 Msa0944220:three_prime_UTR 35.0%
TGAATATGAGAACTAACCTC+AGG - chr6_3:21001838-21001857 None:intergenic 35.0%
AACAAAGGACCAATTGAGAA+TGG - chr6_3:21002169-21002188 None:intergenic 35.0%
CGTAGAATTTATACCTAGGT+TGG - chr6_3:21002200-21002219 None:intergenic 35.0%
GAGTCGTAGAATTTATACCT+AGG - chr6_3:21002204-21002223 None:intergenic 35.0%
TTCTACGACTCATCTTAATG+TGG + chr6_3:21002213-21002232 Msa0944220:intron 35.0%
GATTGTTCAGATTTCCTCAA+TGG - chr6_3:21002422-21002441 None:intergenic 35.0%
CCCTTCTAACTTACATATCT+TGG + chr6_3:21002675-21002694 Msa0944220:intron 35.0%
ATCAGAACGAGAATTCTCAA+TGG - chr6_3:21002795-21002814 None:intergenic 35.0%
TTCTGATGAAAAGCTCCTAA+GGG + chr6_3:21002808-21002827 Msa0944220:CDS 35.0%
! TGCTTGAGAAAAACGTTTTG+TGG + chr6_3:21002948-21002967 Msa0944220:CDS 35.0%
! GCTTGAGAAAAACGTTTTGT+GGG + chr6_3:21002949-21002968 Msa0944220:CDS 35.0%
GAATCCAAGTAATCACACAA+TGG + chr6_3:21003106-21003125 Msa0944220:three_prime_UTR 35.0%
AGAACCATTGTGTGATTACT+TGG - chr6_3:21003113-21003132 None:intergenic 35.0%
! TTAGTATGCTCTTTTGAGCA+AGG + chr6_3:21003206-21003225 Msa0944220:three_prime_UTR 35.0%
! TAGTATGCTCTTTTGAGCAA+GGG + chr6_3:21003207-21003226 Msa0944220:three_prime_UTR 35.0%
AGCAAAAAAGATGTGTTGTG+GGG - chr6_3:21003239-21003258 None:intergenic 35.0%
TATTTAGTCCAGAAGATGAG+AGG + chr6_3:21003381-21003400 Msa0944220:three_prime_UTR 35.0%
TAATCGACGACATCTGAATG+AGG - chr6_3:21001893-21001912 None:intergenic 40.0%
TACTTTGGTGAGCAGTTATC+AGG + chr6_3:21001973-21001992 Msa0944220:CDS 40.0%
TTGCCTTCACTGTTAATGGA+TGG + chr6_3:21001998-21002017 Msa0944220:CDS 40.0%
! TGCCTTCACTGTTAATGGAT+GGG + chr6_3:21001999-21002018 Msa0944220:CDS 40.0%
AATGGATGGGTGCAATCTTA+TGG + chr6_3:21002012-21002031 Msa0944220:CDS 40.0%
ACATCACCATAGATGATTGG+TGG - chr6_3:21002054-21002073 None:intergenic 40.0%
CTCACATCACCATAGATGAT+TGG - chr6_3:21002057-21002076 None:intergenic 40.0%
CAGTAAGCATTCCCTTCATT+GGG - chr6_3:21002137-21002156 None:intergenic 40.0%
ACCAATTGAGAATGGTGACA+GGG - chr6_3:21002161-21002180 None:intergenic 40.0%
! AGGTTGGTCATTACGAACAA+AGG - chr6_3:21002184-21002203 None:intergenic 40.0%
TTGTTCGTAATGACCAACCT+AGG + chr6_3:21002184-21002203 Msa0944220:CDS 40.0%
TACGACTCATCTTAATGTGG+TGG + chr6_3:21002216-21002235 Msa0944220:intron 40.0%
AGCAATCTGATAGCAAGTCT+CGG - chr6_3:21002312-21002331 None:intergenic 40.0%
!! GACTTGCTATCAGATTGCTT+TGG + chr6_3:21002313-21002332 Msa0944220:CDS 40.0%
TCAGATTGCTTTGGCTATCA+AGG + chr6_3:21002322-21002341 Msa0944220:CDS 40.0%
!!! CAGTAATACCGGCTTTTTCA+AGG - chr6_3:21002361-21002380 None:intergenic 40.0%
TCAAAGCAGCCTCATCAATT+TGG - chr6_3:21002388-21002407 None:intergenic 40.0%
ATCTGAACAATCCCACTACT+TGG + chr6_3:21002430-21002449 Msa0944220:CDS 40.0%
! CATTGGTGATCCTGAAAGAA+TGG - chr6_3:21002466-21002485 None:intergenic 40.0%
TCTTTCAGGATCACCAATGT+TGG + chr6_3:21002467-21002486 Msa0944220:CDS 40.0%
CATCCACTCAATCATTGACA+TGG + chr6_3:21002754-21002773 Msa0944220:CDS 40.0%
CATCCATGTCAATGATTGAG+TGG - chr6_3:21002760-21002779 None:intergenic 40.0%
GTTCTGATGAAAAGCTCCTA+AGG + chr6_3:21002807-21002826 Msa0944220:CDS 40.0%
! GTTAAGTATGGTGCTGGAAT+TGG + chr6_3:21002845-21002864 Msa0944220:CDS 40.0%
AGTGAATGTCATAAACTCCG+GGG - chr6_3:21002871-21002890 None:intergenic 40.0%
GAGTGAATGTCATAAACTCC+GGG - chr6_3:21002872-21002891 None:intergenic 40.0%
GGAGTGAATGTCATAAACTC+CGG - chr6_3:21002873-21002892 None:intergenic 40.0%
!! TTCAGTTGGTGGTATTCTTG+GGG - chr6_3:21002894-21002913 None:intergenic 40.0%
!! CTTCAGTTGGTGGTATTCTT+GGG - chr6_3:21002895-21002914 None:intergenic 40.0%
!! TCTTCAGTTGGTGGTATTCT+TGG - chr6_3:21002896-21002915 None:intergenic 40.0%
TCAGCAATTTCTTCAGTTGG+TGG - chr6_3:21002905-21002924 None:intergenic 40.0%
CTCTCAGCAATTTCTTCAGT+TGG - chr6_3:21002908-21002927 None:intergenic 40.0%
CAACTGAAGAAATTGCTGAG+AGG + chr6_3:21002906-21002925 Msa0944220:CDS 40.0%
TTTACTTTCCACTTGCAAGC+TGG - chr6_3:21003066-21003085 None:intergenic 40.0%
GCTTGCAAGTGGAAAGTAAA+TGG + chr6_3:21003066-21003085 Msa0944220:exon 40.0%
! AATCACACAATGGTTCTGCT+TGG + chr6_3:21003116-21003135 Msa0944220:three_prime_UTR 40.0%
TTGAAGTTCCTCTCATCTTC+TGG - chr6_3:21003392-21003411 None:intergenic 40.0%
TAATCGACGACATCTGAATG+AGG - chr6_3:21001893-21001912 None:intergenic 40.0%
TACTTTGGTGAGCAGTTATC+AGG + chr6_3:21001973-21001992 Msa0944220:CDS 40.0%
TTGCCTTCACTGTTAATGGA+TGG + chr6_3:21001998-21002017 Msa0944220:CDS 40.0%
! TGCCTTCACTGTTAATGGAT+GGG + chr6_3:21001999-21002018 Msa0944220:CDS 40.0%
AATGGATGGGTGCAATCTTA+TGG + chr6_3:21002012-21002031 Msa0944220:CDS 40.0%
ACATCACCATAGATGATTGG+TGG - chr6_3:21002054-21002073 None:intergenic 40.0%
CTCACATCACCATAGATGAT+TGG - chr6_3:21002057-21002076 None:intergenic 40.0%
CAGTAAGCATTCCCTTCATT+GGG - chr6_3:21002137-21002156 None:intergenic 40.0%
ACCAATTGAGAATGGTGACA+GGG - chr6_3:21002161-21002180 None:intergenic 40.0%
! AGGTTGGTCATTACGAACAA+AGG - chr6_3:21002184-21002203 None:intergenic 40.0%
TTGTTCGTAATGACCAACCT+AGG + chr6_3:21002184-21002203 Msa0944220:CDS 40.0%
TACGACTCATCTTAATGTGG+TGG + chr6_3:21002216-21002235 Msa0944220:intron 40.0%
AGCAATCTGATAGCAAGTCT+CGG - chr6_3:21002312-21002331 None:intergenic 40.0%
!! GACTTGCTATCAGATTGCTT+TGG + chr6_3:21002313-21002332 Msa0944220:CDS 40.0%
TCAGATTGCTTTGGCTATCA+AGG + chr6_3:21002322-21002341 Msa0944220:CDS 40.0%
!!! CAGTAATACCGGCTTTTTCA+AGG - chr6_3:21002361-21002380 None:intergenic 40.0%
TCAAAGCAGCCTCATCAATT+TGG - chr6_3:21002388-21002407 None:intergenic 40.0%
ATCTGAACAATCCCACTACT+TGG + chr6_3:21002430-21002449 Msa0944220:CDS 40.0%
! CATTGGTGATCCTGAAAGAA+TGG - chr6_3:21002466-21002485 None:intergenic 40.0%
TCTTTCAGGATCACCAATGT+TGG + chr6_3:21002467-21002486 Msa0944220:CDS 40.0%
CATCCACTCAATCATTGACA+TGG + chr6_3:21002754-21002773 Msa0944220:CDS 40.0%
CATCCATGTCAATGATTGAG+TGG - chr6_3:21002760-21002779 None:intergenic 40.0%
GTTCTGATGAAAAGCTCCTA+AGG + chr6_3:21002807-21002826 Msa0944220:CDS 40.0%
! GTTAAGTATGGTGCTGGAAT+TGG + chr6_3:21002845-21002864 Msa0944220:CDS 40.0%
AGTGAATGTCATAAACTCCG+GGG - chr6_3:21002871-21002890 None:intergenic 40.0%
GAGTGAATGTCATAAACTCC+GGG - chr6_3:21002872-21002891 None:intergenic 40.0%
GGAGTGAATGTCATAAACTC+CGG - chr6_3:21002873-21002892 None:intergenic 40.0%
!! TTCAGTTGGTGGTATTCTTG+GGG - chr6_3:21002894-21002913 None:intergenic 40.0%
!! CTTCAGTTGGTGGTATTCTT+GGG - chr6_3:21002895-21002914 None:intergenic 40.0%
!! TCTTCAGTTGGTGGTATTCT+TGG - chr6_3:21002896-21002915 None:intergenic 40.0%
TCAGCAATTTCTTCAGTTGG+TGG - chr6_3:21002905-21002924 None:intergenic 40.0%
CTCTCAGCAATTTCTTCAGT+TGG - chr6_3:21002908-21002927 None:intergenic 40.0%
CAACTGAAGAAATTGCTGAG+AGG + chr6_3:21002906-21002925 Msa0944220:CDS 40.0%
TTTACTTTCCACTTGCAAGC+TGG - chr6_3:21003066-21003085 None:intergenic 40.0%
GCTTGCAAGTGGAAAGTAAA+TGG + chr6_3:21003066-21003085 Msa0944220:exon 40.0%
! AATCACACAATGGTTCTGCT+TGG + chr6_3:21003116-21003135 Msa0944220:three_prime_UTR 40.0%
TTGAAGTTCCTCTCATCTTC+TGG - chr6_3:21003392-21003411 None:intergenic 40.0%
CTGAATGAGGCACAGGAAAT+TGG - chr6_3:21001880-21001899 None:intergenic 45.0%
GGCTTTGCCTTCACTGTTAA+TGG + chr6_3:21001994-21002013 Msa0944220:CDS 45.0%
CACCCATCCATTAACAGTGA+AGG - chr6_3:21002004-21002023 None:intergenic 45.0%
GTGAAGCCACCAATCATCTA+TGG + chr6_3:21002045-21002064 Msa0944220:CDS 45.0%
AGCCATAGATGACCAGAAGA+CGG - chr6_3:21002097-21002116 None:intergenic 45.0%
ATGACCAAACGCCCAATGAA+GGG + chr6_3:21002123-21002142 Msa0944220:CDS 45.0%
CCAGTAAGCATTCCCTTCAT+TGG - chr6_3:21002138-21002157 None:intergenic 45.0%
CCAATGAAGGGAATGCTTAC+TGG + chr6_3:21002135-21002154 Msa0944220:CDS 45.0%
GACCAATTGAGAATGGTGAC+AGG - chr6_3:21002162-21002181 None:intergenic 45.0%
GTCGAAGACCTTGAAAAAGC+CGG + chr6_3:21002350-21002369 Msa0944220:CDS 45.0%
!! ATGAGGCTGCTTTGAGAGAA+GGG + chr6_3:21002393-21002412 Msa0944220:CDS 45.0%
AACAATCCCACTACTTGGAC+TGG + chr6_3:21002435-21002454 Msa0944220:CDS 45.0%
ACAATCCCACTACTTGGACT+GGG + chr6_3:21002436-21002455 Msa0944220:CDS 45.0%
AGTATCCTGCACACCAACAT+TGG - chr6_3:21002483-21002502 None:intergenic 45.0%
! GCACTGACACTTCAGATCAT+TGG + chr6_3:21002533-21002552 Msa0944220:intron 45.0%
GAAGTGAGAGTAGCACATGT+GGG - chr6_3:21002726-21002745 None:intergenic 45.0%
TGAAGTGAGAGTAGCACATG+TGG - chr6_3:21002727-21002746 None:intergenic 45.0%
TTCCGCGATGGAGTTAAGTA+TGG + chr6_3:21002833-21002852 Msa0944220:CDS 45.0%
CACCATACTTAACTCCATCG+CGG - chr6_3:21002838-21002857 None:intergenic 45.0%
GATGGAGTTAAGTATGGTGC+TGG + chr6_3:21002839-21002858 Msa0944220:CDS 45.0%
TTGTGGGTCAATCCTGATTG+TGG + chr6_3:21002965-21002984 Msa0944220:CDS 45.0%
!!! CGAGTTTTGAGACCACAATC+AGG - chr6_3:21002980-21002999 None:intergenic 45.0%
CTGAATGAGGCACAGGAAAT+TGG - chr6_3:21001880-21001899 None:intergenic 45.0%
GGCTTTGCCTTCACTGTTAA+TGG + chr6_3:21001994-21002013 Msa0944220:CDS 45.0%
CACCCATCCATTAACAGTGA+AGG - chr6_3:21002004-21002023 None:intergenic 45.0%
GTGAAGCCACCAATCATCTA+TGG + chr6_3:21002045-21002064 Msa0944220:CDS 45.0%
AGCCATAGATGACCAGAAGA+CGG - chr6_3:21002097-21002116 None:intergenic 45.0%
ATGACCAAACGCCCAATGAA+GGG + chr6_3:21002123-21002142 Msa0944220:CDS 45.0%
CCAGTAAGCATTCCCTTCAT+TGG - chr6_3:21002138-21002157 None:intergenic 45.0%
CCAATGAAGGGAATGCTTAC+TGG + chr6_3:21002135-21002154 Msa0944220:CDS 45.0%
GACCAATTGAGAATGGTGAC+AGG - chr6_3:21002162-21002181 None:intergenic 45.0%
GTCGAAGACCTTGAAAAAGC+CGG + chr6_3:21002350-21002369 Msa0944220:CDS 45.0%
!! ATGAGGCTGCTTTGAGAGAA+GGG + chr6_3:21002393-21002412 Msa0944220:CDS 45.0%
AACAATCCCACTACTTGGAC+TGG + chr6_3:21002435-21002454 Msa0944220:CDS 45.0%
ACAATCCCACTACTTGGACT+GGG + chr6_3:21002436-21002455 Msa0944220:CDS 45.0%
AGTATCCTGCACACCAACAT+TGG - chr6_3:21002483-21002502 None:intergenic 45.0%
! GCACTGACACTTCAGATCAT+TGG + chr6_3:21002533-21002552 Msa0944220:intron 45.0%
GAAGTGAGAGTAGCACATGT+GGG - chr6_3:21002726-21002745 None:intergenic 45.0%
TGAAGTGAGAGTAGCACATG+TGG - chr6_3:21002727-21002746 None:intergenic 45.0%
TTCCGCGATGGAGTTAAGTA+TGG + chr6_3:21002833-21002852 Msa0944220:CDS 45.0%
CACCATACTTAACTCCATCG+CGG - chr6_3:21002838-21002857 None:intergenic 45.0%
GATGGAGTTAAGTATGGTGC+TGG + chr6_3:21002839-21002858 Msa0944220:CDS 45.0%
TTGTGGGTCAATCCTGATTG+TGG + chr6_3:21002965-21002984 Msa0944220:CDS 45.0%
!!! CGAGTTTTGAGACCACAATC+AGG - chr6_3:21002980-21002999 None:intergenic 45.0%
GAGGCACAGGAAATTGGACA+AGG - chr6_3:21001874-21001893 None:intergenic 50.0%
ACGACATCTGAATGAGGCAC+AGG - chr6_3:21001887-21001906 None:intergenic 50.0%
AGACGGTCATTGCCTTAGGA+CGG - chr6_3:21002080-21002099 None:intergenic 50.0%
CAGAAGACGGTCATTGCCTT+AGG - chr6_3:21002084-21002103 None:intergenic 50.0%
CTAAGGCAATGACCGTCTTC+TGG + chr6_3:21002082-21002101 Msa0944220:CDS 50.0%
GACCGTCTTCTGGTCATCTA+TGG + chr6_3:21002092-21002111 Msa0944220:CDS 50.0%
CATGACCAAACGCCCAATGA+AGG + chr6_3:21002122-21002141 Msa0944220:CDS 50.0%
CATTCCCTTCATTGGGCGTT+TGG - chr6_3:21002130-21002149 None:intergenic 50.0%
GCCCTGTCACCATTCTCAAT+TGG + chr6_3:21002157-21002176 Msa0944220:CDS 50.0%
!! GATGAGGCTGCTTTGAGAGA+AGG + chr6_3:21002392-21002411 Msa0944220:CDS 50.0%
TGAGAGAAGGGTTGCCATTG+AGG + chr6_3:21002405-21002424 Msa0944220:CDS 50.0%
ACTGGGCTGTCCATTCTTTC+AGG + chr6_3:21002453-21002472 Msa0944220:CDS 50.0%
GATCACCAATGTTGGTGTGC+AGG + chr6_3:21002475-21002494 Msa0944220:CDS 50.0%
TGGTGTGCAGGATACTACTC+AGG + chr6_3:21002487-21002506 Msa0944220:CDS 50.0%
TTACGTGTCAGTGTCGTGTC+TGG + chr6_3:21002605-21002624 Msa0944220:intron 50.0%
CAAAACTCGCAAGTACACCG+AGG + chr6_3:21002991-21003010 Msa0944220:CDS 50.0%
TTCCACTTGCAAGCTGGTTG+CGG - chr6_3:21003060-21003079 None:intergenic 50.0%
GAGGCACAGGAAATTGGACA+AGG - chr6_3:21001874-21001893 None:intergenic 50.0%
ACGACATCTGAATGAGGCAC+AGG - chr6_3:21001887-21001906 None:intergenic 50.0%
AGACGGTCATTGCCTTAGGA+CGG - chr6_3:21002080-21002099 None:intergenic 50.0%
CAGAAGACGGTCATTGCCTT+AGG - chr6_3:21002084-21002103 None:intergenic 50.0%
CTAAGGCAATGACCGTCTTC+TGG + chr6_3:21002082-21002101 Msa0944220:CDS 50.0%
GACCGTCTTCTGGTCATCTA+TGG + chr6_3:21002092-21002111 Msa0944220:CDS 50.0%
CATGACCAAACGCCCAATGA+AGG + chr6_3:21002122-21002141 Msa0944220:CDS 50.0%
CATTCCCTTCATTGGGCGTT+TGG - chr6_3:21002130-21002149 None:intergenic 50.0%
GCCCTGTCACCATTCTCAAT+TGG + chr6_3:21002157-21002176 Msa0944220:CDS 50.0%
!! GATGAGGCTGCTTTGAGAGA+AGG + chr6_3:21002392-21002411 Msa0944220:CDS 50.0%
TGAGAGAAGGGTTGCCATTG+AGG + chr6_3:21002405-21002424 Msa0944220:CDS 50.0%
ACTGGGCTGTCCATTCTTTC+AGG + chr6_3:21002453-21002472 Msa0944220:CDS 50.0%
GATCACCAATGTTGGTGTGC+AGG + chr6_3:21002475-21002494 Msa0944220:CDS 50.0%
TGGTGTGCAGGATACTACTC+AGG + chr6_3:21002487-21002506 Msa0944220:CDS 50.0%
TTACGTGTCAGTGTCGTGTC+TGG + chr6_3:21002605-21002624 Msa0944220:intron 50.0%
CAAAACTCGCAAGTACACCG+AGG + chr6_3:21002991-21003010 Msa0944220:CDS 50.0%
TTCCACTTGCAAGCTGGTTG+CGG - chr6_3:21003060-21003079 None:intergenic 50.0%
! TGGTGATGTGAGCCGTCCTA+AGG + chr6_3:21002065-21002084 Msa0944220:CDS 55.0%
GACAGCCCAGTCCAAGTAGT+GGG - chr6_3:21002444-21002463 None:intergenic 55.0%
CAGTGTGAGCCACTCGTCAA+TGG + chr6_3:21002562-21002581 Msa0944220:intron 55.0%
GTGTGTGAGCCATTGACGAG+TGG - chr6_3:21002574-21002593 None:intergenic 55.0%
GAGAGTAGCACATGTGGGTG+TGG - chr6_3:21002721-21002740 None:intergenic 55.0%
! TATGGTGCTGGAATTGGCCC+CGG + chr6_3:21002851-21002870 Msa0944220:CDS 55.0%
TGTGAGAGCTGGGTTCACCT+CGG - chr6_3:21003011-21003030 None:intergenic 55.0%
CAACCATGGCTGTGAGAGCT+GGG - chr6_3:21003021-21003040 None:intergenic 55.0%
TCAACCATGGCTGTGAGAGC+TGG - chr6_3:21003022-21003041 None:intergenic 55.0%
AGCTCTCACAGCCATGGTTG+AGG + chr6_3:21003021-21003040 Msa0944220:CDS 55.0%
! GCGTTTTGTGGCCTCAACCA+TGG - chr6_3:21003035-21003054 None:intergenic 55.0%
! CTGGTTGCGGATGCGTTTTG+TGG - chr6_3:21003047-21003066 None:intergenic 55.0%
ATCCGCAACCAGCTTGCAAG+TGG + chr6_3:21003055-21003074 Msa0944220:CDS 55.0%
AACCTCAGGCTCTCCGTGTA+CGG - chr6_3:21001824-21001843 None:intergenic 55.0%
! TGGTGATGTGAGCCGTCCTA+AGG + chr6_3:21002065-21002084 Msa0944220:CDS 55.0%
GACAGCCCAGTCCAAGTAGT+GGG - chr6_3:21002444-21002463 None:intergenic 55.0%
CAGTGTGAGCCACTCGTCAA+TGG + chr6_3:21002562-21002581 Msa0944220:intron 55.0%
GTGTGTGAGCCATTGACGAG+TGG - chr6_3:21002574-21002593 None:intergenic 55.0%
GAGAGTAGCACATGTGGGTG+TGG - chr6_3:21002721-21002740 None:intergenic 55.0%
! TATGGTGCTGGAATTGGCCC+CGG + chr6_3:21002851-21002870 Msa0944220:CDS 55.0%
TGTGAGAGCTGGGTTCACCT+CGG - chr6_3:21003011-21003030 None:intergenic 55.0%
CAACCATGGCTGTGAGAGCT+GGG - chr6_3:21003021-21003040 None:intergenic 55.0%
TCAACCATGGCTGTGAGAGC+TGG - chr6_3:21003022-21003041 None:intergenic 55.0%
AGCTCTCACAGCCATGGTTG+AGG + chr6_3:21003021-21003040 Msa0944220:CDS 55.0%
! GCGTTTTGTGGCCTCAACCA+TGG - chr6_3:21003035-21003054 None:intergenic 55.0%
! CTGGTTGCGGATGCGTTTTG+TGG - chr6_3:21003047-21003066 None:intergenic 55.0%
ATCCGCAACCAGCTTGCAAG+TGG + chr6_3:21003055-21003074 Msa0944220:CDS 55.0%
GGACAGCCCAGTCCAAGTAG+TGG - chr6_3:21002445-21002464 None:intergenic 60.0%
!! CTCCTAAGGGTGTTCCGCGA+TGG + chr6_3:21002821-21002840 Msa0944220:CDS 60.0%
CTCCATCGCGGAACACCCTT+AGG - chr6_3:21002826-21002845 None:intergenic 60.0%
GAACCCAGCTCTCACAGCCA+TGG + chr6_3:21003015-21003034 Msa0944220:CDS 60.0%
GGACAGCCCAGTCCAAGTAG+TGG - chr6_3:21002445-21002464 None:intergenic 60.0%
!! CTCCTAAGGGTGTTCCGCGA+TGG + chr6_3:21002821-21002840 Msa0944220:CDS 60.0%
CTCCATCGCGGAACACCCTT+AGG - chr6_3:21002826-21002845 None:intergenic 60.0%
GAACCCAGCTCTCACAGCCA+TGG + chr6_3:21003015-21003034 Msa0944220:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 21001823 21003445 21001823 ID=Msa0944220;Name=Msa0944220
chr6_3 mRNA 21001823 21003445 21001823 ID=Msa0944220-mRNA-1;Parent=Msa0944220;Name=Msa0944220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=27|1|1|1|0.66|0.5|4|361|270
chr6_3 exon 21001823 21001840 21001823 ID=Msa0944220-mRNA-1:exon:6234;Parent=Msa0944220-mRNA-1
chr6_3 exon 21001955 21002205 21001955 ID=Msa0944220-mRNA-1:exon:6235;Parent=Msa0944220-mRNA-1
chr6_3 exon 21002307 21002508 21002307 ID=Msa0944220-mRNA-1:exon:6236;Parent=Msa0944220-mRNA-1
chr6_3 exon 21002716 21003445 21002716 ID=Msa0944220-mRNA-1:exon:6237;Parent=Msa0944220-mRNA-1
chr6_3 five_prime_UTR 21001823 21001840 21001823 ID=Msa0944220-mRNA-1:five_prime_utr;Parent=Msa0944220-mRNA-1
chr6_3 five_prime_UTR 21001955 21001963 21001955 ID=Msa0944220-mRNA-1:five_prime_utr;Parent=Msa0944220-mRNA-1
chr6_3 CDS 21001964 21002205 21001964 ID=Msa0944220-mRNA-1:cds;Parent=Msa0944220-mRNA-1
chr6_3 CDS 21002307 21002508 21002307 ID=Msa0944220-mRNA-1:cds;Parent=Msa0944220-mRNA-1
chr6_3 CDS 21002716 21003084 21002716 ID=Msa0944220-mRNA-1:cds;Parent=Msa0944220-mRNA-1
chr6_3 three_prime_UTR 21003085 21003445 21003085 ID=Msa0944220-mRNA-1:three_prime_utr;Parent=Msa0944220-mRNA-1
Gene Sequence

>Msa0944220

ATGGTTGAGTACTTTGGTGAGCAGTTATCAGGCTTTGCCTTCACTGTTAATGGATGGGTGCAATCTTATGGATCTCGCTGTGTGAAGCCACCAATCATCTATGGTGATGTGAGCCGTCCTAAGGCAATGACCGTCTTCTGGTCATCTATGGCTCAGAGCATGACCAAACGCCCAATGAAGGGAATGCTTACTGGCCCTGTCACCATTCTCAATTGGTCCTTTGTTCGTAATGACCAACCTAGATCCGAGACTTGCTATCAGATTGCTTTGGCTATCAAGGATGAAGTCGAAGACCTTGAAAAAGCCGGTATTACTGTTATCCAAATTGATGAGGCTGCTTTGAGAGAAGGGTTGCCATTGAGGAAATCTGAACAATCCCACTACTTGGACTGGGCTGTCCATTCTTTCAGGATCACCAATGTTGGTGTGCAGGATACTACTCAGATCCACACCCACATGTGCTACTCTCACTTCAATGATATCATCCACTCAATCATTGACATGGATGCTGATGTGATCACCATTGAGAATTCTCGTTCTGATGAAAAGCTCCTAAGGGTGTTCCGCGATGGAGTTAAGTATGGTGCTGGAATTGGCCCCGGAGTTTATGACATTCACTCCCCAAGAATACCACCAACTGAAGAAATTGCTGAGAGGATCAACAAGATGCTTGCAGTGCTTGAGAAAAACGTTTTGTGGGTCAATCCTGATTGTGGTCTCAAAACTCGCAAGTACACCGAGGTGAACCCAGCTCTCACAGCCATGGTTGAGGCCACAAAACGCATCCGCAACCAGCTTGCAAGTGGAAAGTAA
Protein sequence

>Msa0944220

MVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEQSHYLDWAVHSFRITNVGVQDTTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLRVFRDGVKYGAGIGPGVYDIHSPRIPPTEEIAERINKMLAVLEKNVLWVNPDCGLKTRKYTEVNPALTAMVEATKRIRNQLASGK*