AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1412710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1412710 MtrunA17_Chr1g0193671 94.977 219 11 0 1 219 1 219 1.38e-152 422
Msa1412710 MtrunA17_Chr1g0193421 70.500 200 59 0 19 218 20 219 8.00e-100 288
Msa1412710 MtrunA17_Chr7g0271211 68.878 196 61 0 22 217 19 214 2.40e-94 275
Msa1412710 MtrunA17_Chr2g0285871 47.596 208 102 3 11 213 10 215 5.90e-60 187
Msa1412710 MtrunA17_Chr4g0007041 47.115 208 103 3 11 213 10 215 8.08e-60 187
Msa1412710 MtrunA17_Chr1g0189841 46.009 213 109 3 6 213 5 216 1.14e-59 187
Msa1412710 MtrunA17_Chr4g0007051 47.115 208 103 3 11 213 10 215 1.16e-59 187
Msa1412710 MtrunA17_Chr4g0007031 47.115 208 103 3 11 213 10 215 1.21e-59 186
Msa1412710 MtrunA17_Chr5g0419971 47.115 208 103 3 11 213 10 215 1.21e-59 186
Msa1412710 MtrunA17_Chr5g0419981 47.115 208 103 3 11 213 10 215 1.21e-59 186
Msa1412710 MtrunA17_Chr6g0449751 47.393 211 104 4 8 213 6 214 4.99e-58 182
Msa1412710 MtrunA17_Chr6g0449771 48.259 201 98 3 17 213 16 214 1.30e-57 181
Msa1412710 MtrunA17_Chr6g0449761 48.259 201 98 3 17 213 16 214 2.62e-57 181
Msa1412710 MtrunA17_Chr6g0449731 48.259 201 98 3 17 213 16 214 3.46e-57 180
Msa1412710 MtrunA17_Chr6g0449791 49.010 202 95 4 8 203 6 205 3.00e-56 177
Msa1412710 MtrunA17_Chr1g0156131 42.927 205 109 2 11 213 10 208 1.07e-55 176
Msa1412710 MtrunA17_Chr2g0292831 44.898 196 103 2 20 213 19 211 3.81e-55 175
Msa1412710 MtrunA17_Chr1g0210761 42.857 210 113 3 7 216 7 209 7.92e-55 174
Msa1412710 MtrunA17_Chr2g0292901 43.216 199 107 3 18 213 17 212 1.20e-51 166
Msa1412710 MtrunA17_Chr6g0449781 43.128 211 112 5 8 213 6 213 3.20e-51 165
Msa1412710 MtrunA17_Chr5g0414971 43.077 195 110 1 22 215 26 220 2.25e-49 160
Msa1412710 MtrunA17_Chr1g0178711 38.009 221 126 5 6 218 8 225 1.77e-48 158
Msa1412710 MtrunA17_Chr2g0292861 43.860 171 93 2 46 213 7 177 1.46e-45 149
Msa1412710 MtrunA17_Chr2g0292841 43.860 171 93 2 46 213 7 177 6.81e-45 147
Msa1412710 MtrunA17_Chr8g0335851 42.703 185 102 3 6 188 10 192 1.94e-40 137
Msa1412710 MtrunA17_Chr8g0343911 37.838 185 111 1 8 192 4 184 4.85e-38 131
Msa1412710 MtrunA17_Chr2g0321581 39.394 198 96 6 19 211 23 201 8.30e-38 130
Msa1412710 MtrunA17_Chr2g0321601 39.394 198 96 6 19 211 22 200 8.43e-38 130
Msa1412710 MtrunA17_Chr2g0321591 39.394 198 96 6 19 211 23 201 1.15e-37 130
Msa1412710 MtrunA17_Chr3g0130241 37.864 206 122 4 9 210 73 276 3.57e-37 131
Msa1412710 MtrunA17_Chr8g0343954 35.417 192 120 3 26 217 21 208 2.27e-36 127
Msa1412710 MtrunA17_Chr8g0343934 34.673 199 126 3 19 217 14 208 2.43e-36 127
Msa1412710 MtrunA17_Chr2g0292911 51.282 117 55 1 46 160 7 123 1.34e-35 122
Msa1412710 MtrunA17_Chr2g0313961 36.765 204 125 3 11 214 6 205 4.91e-35 123
Msa1412710 MtrunA17_Chr2g0297241 37.368 190 115 3 26 215 21 206 7.00e-35 123
Msa1412710 MtrunA17_Chr4g0026191 43.284 134 73 2 79 211 71 202 1.01e-34 122
Msa1412710 MtrunA17_Chr2g0302171 37.019 208 127 3 8 215 3 206 1.19e-34 122
Msa1412710 MtrunA17_Chr4g0026201 43.284 134 73 2 79 211 71 202 1.38e-34 122
Msa1412710 MtrunA17_Chr3g0084511 36.316 190 117 3 26 215 20 205 1.61e-34 122
Msa1412710 MtrunA17_Chr4g0003361 38.942 208 109 8 9 213 8 200 2.96e-34 121
Msa1412710 MtrunA17_Chr2g0297231 36.842 190 116 3 26 215 21 206 3.45e-34 121
Msa1412710 MtrunA17_Chr8g0343961 32.381 210 134 4 8 217 7 208 4.21e-34 121
Msa1412710 MtrunA17_Chr5g0411961 33.175 211 131 4 8 215 3 206 9.20e-34 120
Msa1412710 MtrunA17_Chr3g0084521 36.316 190 117 3 26 215 20 205 1.35e-33 119
Msa1412710 MtrunA17_Chr3g0084531 35.500 200 119 4 19 215 14 206 2.36e-33 119
Msa1412710 MtrunA17_Chr2g0300561 36.559 186 110 3 29 211 23 203 4.42e-33 118
Msa1412710 MtrunA17_Chr2g0300581 34.300 207 129 3 6 212 5 204 1.74e-32 117
Msa1412710 MtrunA17_Chr2g0300591 34.466 206 128 3 6 211 5 203 2.43e-32 116
Msa1412710 MtrunA17_Chr2g0301611 34.135 208 129 4 8 215 7 206 4.90e-32 115
Msa1412710 MtrunA17_Chr8g0344041 35.437 206 122 6 11 213 11 208 5.40e-31 113
Msa1412710 MtrunA17_Chr8g0344051 38.278 209 113 6 8 208 7 207 3.49e-30 110
Msa1412710 MtrunA17_Chr2g0302541 35.263 190 119 3 26 215 21 206 4.41e-30 110
Msa1412710 MtrunA17_Chr4g0012651 35.450 189 118 4 27 215 22 206 9.41e-30 109
Msa1412710 MtrunA17_Chr7g0217811 35.450 189 118 4 27 215 22 206 1.08e-29 109
Msa1412710 MtrunA17_Chr8g0344021 35.784 204 122 4 9 211 10 205 2.43e-29 108
Msa1412710 MtrunA17_Chr8g0343921 32.039 206 132 3 6 211 3 200 6.16e-28 105
Msa1412710 MtrunA17_Chr2g0286171 32.227 211 134 6 5 213 4 207 1.97e-27 103
Msa1412710 MtrunA17_Chr8g0344031 34.343 198 120 6 19 213 16 206 2.48e-26 100
Msa1412710 MtrunA17_Chr3g0084551 33.171 205 125 6 8 211 3 196 2.55e-25 99.0
Msa1412710 MtrunA17_Chr3g0115141 33.155 187 117 5 28 213 26 205 7.27e-25 97.1
Msa1412710 MtrunA17_Chr3g0084501 39.252 107 58 3 8 111 3 105 5.87e-15 68.6
Msa1412710 MtrunA17_Chr2g0292881 52.632 57 25 1 94 148 1 57 1.08e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1412710 AT3G62020.1 70.000 220 63 2 1 218 1 219 1.75e-108 311
Msa1412710 AT1G02335.1 65.403 211 73 0 8 218 9 219 7.11e-102 294
Msa1412710 AT1G09560.1 70.202 198 59 0 21 218 22 219 2.38e-99 288
Msa1412710 AT3G62020.2 74.863 183 43 2 38 218 9 190 2.09e-98 284
Msa1412710 AT5G26700.1 59.794 194 75 1 25 218 23 213 3.24e-86 254
Msa1412710 AT3G05930.1 53.425 219 101 1 1 218 1 219 2.29e-78 234
Msa1412710 AT1G18980.1 45.933 209 111 1 7 215 13 219 5.23e-64 198
Msa1412710 AT1G18970.1 46.411 209 110 1 7 215 13 219 2.18e-63 196
Msa1412710 AT5G38910.1 48.558 208 103 2 11 214 12 219 5.56e-62 193
Msa1412710 AT4G14630.1 50.000 198 94 2 21 213 24 221 2.44e-61 191
Msa1412710 AT5G39160.1 45.498 211 111 2 9 215 10 220 2.85e-61 191
Msa1412710 AT5G39190.1 45.498 211 111 2 9 215 10 220 4.91e-61 191
Msa1412710 AT5G39130.1 45.498 211 111 2 9 215 10 220 5.24e-61 191
Msa1412710 AT5G39150.1 45.972 211 109 2 8 213 8 218 7.68e-61 190
Msa1412710 AT5G38930.1 49.246 199 97 2 19 213 22 220 1.71e-60 189
Msa1412710 AT5G39180.1 45.972 211 109 2 8 213 8 218 2.49e-60 189
Msa1412710 AT5G39120.1 47.980 198 98 2 21 213 21 218 2.68e-60 189
Msa1412710 AT5G38940.1 48.980 196 96 2 22 213 25 220 3.59e-60 188
Msa1412710 AT3G05950.1 48.095 210 103 3 12 215 13 222 4.50e-60 188
Msa1412710 AT5G38940.2 48.980 196 96 2 22 213 49 244 4.67e-60 189
Msa1412710 AT5G39110.1 46.602 206 105 2 13 213 14 219 6.32e-60 187
Msa1412710 AT5G38960.1 48.000 200 99 3 21 215 24 223 1.85e-59 186
Msa1412710 AT3G04200.1 45.283 212 110 3 11 216 13 224 4.58e-59 186
Msa1412710 AT5G39160.3 43.810 210 112 2 9 215 10 216 9.18e-58 182
Msa1412710 AT3G04170.1 43.662 213 111 4 11 217 12 221 1.57e-51 166
Msa1412710 AT3G04180.1 43.069 202 105 4 17 211 20 218 2.95e-50 163
Msa1412710 AT5G39160.2 40.000 210 102 2 9 215 10 198 4.03e-50 162
Msa1412710 AT5G39190.2 40.000 210 102 2 9 215 10 198 6.43e-50 161
Msa1412710 AT3G04150.1 41.063 207 116 3 11 211 12 218 7.32e-50 162
Msa1412710 AT3G04190.1 42.211 199 111 2 17 211 20 218 4.24e-49 160
Msa1412710 AT3G04150.2 39.091 220 115 3 11 211 12 231 1.63e-48 159
Msa1412710 AT3G10080.1 40.887 203 112 4 19 216 22 221 3.85e-47 155
Msa1412710 AT3G10080.2 40.887 203 112 4 19 216 93 292 1.92e-46 155
Msa1412710 AT5G38910.2 40.976 205 84 2 11 213 12 181 2.08e-42 142
Msa1412710 AT1G74820.1 36.493 211 127 2 8 213 16 224 4.44e-42 142
Msa1412710 AT1G72610.1 36.559 186 116 1 26 211 18 201 4.88e-39 134
Msa1412710 AT1G10460.1 38.235 204 109 5 22 214 18 215 1.79e-38 132
Msa1412710 AT5G61750.1 39.535 172 102 1 11 180 7 178 1.32e-37 130
Msa1412710 AT5G20630.1 35.784 204 125 2 8 211 7 204 1.98e-36 127
Msa1412710 AT5G38950.1 39.744 78 45 1 143 218 2 79 1.20e-12 62.4

Find 62 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATAAATTAAGTCTTCTT+AGG 0.231546 tig0023943:+33729 None:intergenic
TGTACTTGTAACATTTATTA+TGG 0.237046 tig0023943:-33607 Msa1412710:three_prime_UTR
TGATAATGCACCAACCCTTT+TGG 0.273835 tig0023943:+33930 None:intergenic
CAACAACACTGTAGGATCTT+TGG 0.279663 tig0023943:-34171 Msa1412710:CDS
TAATCATGTCACCAACAAAT+TGG 0.290827 tig0023943:-34975 Msa1412710:exon
CAAAGGTCTTGAAGTGTATC+AGG 0.335291 tig0023943:+34901 None:intergenic
GTGCATTATCAGAAGAATAA+TGG 0.349181 tig0023943:-33918 Msa1412710:CDS
TGCATTATCAGAAGAATAAT+GGG 0.349638 tig0023943:-33917 Msa1412710:CDS
TTGCTTTGAATTTAAGTTGA+AGG 0.354144 tig0023943:-35205 Msa1412710:five_prime_UTR
CTAATACTTACCATCACATT+TGG 0.392691 tig0023943:-35174 Msa1412710:five_prime_UTR
CTTTCAGATTGATACTAAAC+AGG 0.392793 tig0023943:-33778 Msa1412710:CDS
TATGTAGGTGTTAAGGTGAA+TGG 0.396371 tig0023943:-34269 Msa1412710:intron
ACAGTGTTGTTGACAACCTT+AGG 0.398287 tig0023943:+34182 None:intergenic
CACGAGGGTGTGTGTGTGGC+GGG 0.405545 tig0023943:+34060 None:intergenic
GAAATCTTTGTGTTCCCAAA+AGG 0.405554 tig0023943:-33945 Msa1412710:CDS
TCCTGATGATGTTCTTGCTC+AGG 0.416572 tig0023943:-33802 Msa1412710:CDS
TGTGTAGCTCTTCCATCATC+AGG 0.427639 tig0023943:-34880 Msa1412710:intron
AAGAGAGAAGAAAGTTGAGT+TGG 0.430179 tig0023943:+35075 None:intergenic
TTAGTTAGTTCGTTGGTTGC+TGG 0.433772 tig0023943:+35298 None:intergenic
TGTGGCGGGTTAAGACCATT+AGG 0.441756 tig0023943:+34074 None:intergenic
TAGTTAGTTCGTTGGTTGCT+GGG 0.450959 tig0023943:+35299 None:intergenic
TAATTTATAATATTCAAGAA+AGG 0.460213 tig0023943:-33717 Msa1412710:three_prime_UTR
TTTGAAATAATAATTCTTGA+AGG 0.461623 tig0023943:-33644 Msa1412710:three_prime_UTR
TGCGTTAGATCAATAATGAA+TGG 0.462747 tig0023943:+35037 None:intergenic
AGAATAGACTACAAACCTAA+TGG 0.465489 tig0023943:-34089 Msa1412710:CDS
GCCTGAGCAAGAACATCATC+AGG 0.472294 tig0023943:+33801 None:intergenic
CAGTGTTGTTGACAACCTTA+GGG 0.477171 tig0023943:+34183 None:intergenic
CAGTCCAATGAAAAGAGGTT+TGG 0.477909 tig0023943:+35338 None:intergenic
AATTTATAATATTCAAGAAA+GGG 0.481410 tig0023943:-33716 Msa1412710:three_prime_UTR
GAGAAGAAAGTTGAGTTGGA+TGG 0.485875 tig0023943:+35079 None:intergenic
TTGAAATAATAATTCTTGAA+GGG 0.488406 tig0023943:-33643 Msa1412710:three_prime_UTR
ATTGACAGTCCAATGAAAAG+AGG 0.492580 tig0023943:+35333 None:intergenic
AAATCTTTGTGTTCCCAAAA+GGG 0.493371 tig0023943:-33944 Msa1412710:CDS
CTTTGTGTTCCCAAAAGGGT+TGG 0.498869 tig0023943:-33940 Msa1412710:CDS
AATTCTATGAAGTAGCTAGA+AGG 0.501551 tig0023943:-33491 Msa1412710:three_prime_UTR
CATTATACATTACCTGATGA+TGG 0.506437 tig0023943:+34868 None:intergenic
ATTCCTGGACTCAACACATT+GGG 0.515180 tig0023943:-34125 Msa1412710:CDS
GCACGAGGGTGTGTGTGTGG+CGG 0.527458 tig0023943:+34059 None:intergenic
TTATATATGGCATAAGGCAA+TGG 0.532581 tig0023943:+35128 None:intergenic
ACTCCCAATGTGTTGAGTCC+AGG 0.538199 tig0023943:+34122 None:intergenic
ATACATATTAGTTAGTTCGT+TGG 0.547017 tig0023943:+35291 None:intergenic
GTTGTTATTGATAGAGTGGA+TGG 0.548602 tig0023943:+35150 None:intergenic
TATGTCTTATGTAGGTGTTA+AGG 0.563003 tig0023943:-34276 Msa1412710:intron
TTGGAAGGTGAATTAGATGT+AGG 0.564991 tig0023943:-34017 Msa1412710:CDS
AATTCCTGGACTCAACACAT+TGG 0.570200 tig0023943:-34126 Msa1412710:CDS
TGTATCAGGATCAGAAGCGA+AGG 0.579047 tig0023943:+34915 None:intergenic
AGAAGCGAAGGTAGATGAGA+TGG 0.579500 tig0023943:+34927 None:intergenic
TGATGGTATAGCAAACCCTA+AGG 0.582336 tig0023943:-34198 Msa1412710:CDS
GAAACAATTGACAAAGTACC+AGG 0.582389 tig0023943:+33852 None:intergenic
AATGGTTGTTATTGATAGAG+TGG 0.584311 tig0023943:+35146 None:intergenic
GGTGAATGGATTTGCATGCA+AGG 0.593738 tig0023943:-34255 Msa1412710:CDS
CTGTTTAGTATCAATCTGAA+AGG 0.609012 tig0023943:+33779 None:intergenic
TCTGTTTGACTCTACACCAA+CGG 0.619003 tig0023943:-33826 Msa1412710:CDS
GTGGCACGAGGGTGTGTGTG+TGG 0.621879 tig0023943:+34056 None:intergenic
AGAACATCATCAGGAACCGT+TGG 0.626429 tig0023943:+33810 None:intergenic
AAGGTTGTCAACAACACTGT+AGG 0.630939 tig0023943:-34179 Msa1412710:CDS
AAAACAACCTCAGTGGCACG+AGG 0.637231 tig0023943:+34044 None:intergenic
CACACACCCTCGTGCCACTG+AGG 0.678341 tig0023943:-34051 Msa1412710:CDS
AGTGGATGGACCAAATGTGA+TGG 0.697546 tig0023943:+35164 None:intergenic
AAACAACCTCAGTGGCACGA+GGG 0.703640 tig0023943:+34045 None:intergenic
GCATTATCAGAAGAATAATG+GGG 0.712368 tig0023943:-33916 Msa1412710:CDS
ATGATGGAAGAGCTACACAA+AGG 0.722041 tig0023943:+34884 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATACGATTTTATTTATATA+TGG + tig0023943:33617-33636 None:intergenic 10.0%
!! TAATTTATAATATTCAAGAA+AGG - tig0023943:35012-35031 Msa1412710:five_prime_UTR 10.0%
!! AATTTATAATATTCAAGAAA+GGG - tig0023943:35013-35032 Msa1412710:five_prime_UTR 10.0%
!!! AATACGATTTTATTTATATA+TGG + tig0023943:33617-33636 None:intergenic 10.0%
!! TAATTTATAATATTCAAGAA+AGG - tig0023943:35012-35031 Msa1412710:five_prime_UTR 10.0%
!! AATTTATAATATTCAAGAAA+GGG - tig0023943:35013-35032 Msa1412710:five_prime_UTR 10.0%
!!! TTTTATTTATATATGGCATA+AGG + tig0023943:33610-33629 None:intergenic 15.0%
!!! GTTATTTTGTTTCAAAAAAA+TGG + tig0023943:33732-33751 None:intergenic 15.0%
!!! TACTACTATTATCATTTTTT+TGG - tig0023943:33929-33948 Msa1412710:CDS 15.0%
!! TTATATAAAAATAGTTGAAC+TGG - tig0023943:34343-34362 Msa1412710:intron 15.0%
!! ATTATAAATTAAGTCTTCTT+AGG + tig0023943:35003-35022 None:intergenic 15.0%
!!! TTTGAAATAATAATTCTTGA+AGG - tig0023943:35085-35104 Msa1412710:five_prime_UTR 15.0%
!! TTGAAATAATAATTCTTGAA+GGG - tig0023943:35086-35105 Msa1412710:five_prime_UTR 15.0%
!!! TTTTATTTATATATGGCATA+AGG + tig0023943:33610-33629 None:intergenic 15.0%
!!! GTTATTTTGTTTCAAAAAAA+TGG + tig0023943:33732-33751 None:intergenic 15.0%
!!! TACTACTATTATCATTTTTT+TGG - tig0023943:33929-33948 Msa1412710:CDS 15.0%
!! TTATATAAAAATAGTTGAAC+TGG - tig0023943:34343-34362 Msa1412710:intron 15.0%
!! ATTATAAATTAAGTCTTCTT+AGG + tig0023943:35003-35022 None:intergenic 15.0%
!!! TTTGAAATAATAATTCTTGA+AGG - tig0023943:35085-35104 Msa1412710:five_prime_UTR 15.0%
!! TTGAAATAATAATTCTTGAA+GGG - tig0023943:35086-35105 Msa1412710:five_prime_UTR 15.0%
!! AAAAAAATTGTCCAATTTGT+TGG + tig0023943:33768-33787 None:intergenic 20.0%
!! CAAAGTTATGTCTAATATTA+TGG - tig0023943:34430-34449 Msa1412710:intron 20.0%
!! ATTTCTAAGTCCATAAAAAA+AGG - tig0023943:34760-34779 Msa1412710:intron 20.0%
!!! AAAGATTTCACCTTTTTTTA+TGG + tig0023943:34773-34792 None:intergenic 20.0%
!! TGTACTTGTAACATTTATTA+TGG - tig0023943:35122-35141 Msa1412710:five_prime_UTR 20.0%
!! AAAAAAATTGTCCAATTTGT+TGG + tig0023943:33768-33787 None:intergenic 20.0%
!! CAAAGTTATGTCTAATATTA+TGG - tig0023943:34430-34449 Msa1412710:intron 20.0%
!! ATTTCTAAGTCCATAAAAAA+AGG - tig0023943:34760-34779 Msa1412710:intron 20.0%
!!! AAAGATTTCACCTTTTTTTA+TGG + tig0023943:34773-34792 None:intergenic 20.0%
!! TGTACTTGTAACATTTATTA+TGG - tig0023943:35122-35141 Msa1412710:five_prime_UTR 20.0%
! ATACATATTAGTTAGTTCGT+TGG + tig0023943:33441-33460 None:intergenic 25.0%
!!! TTGCTTTGAATTTAAGTTGA+AGG - tig0023943:33524-33543 Msa1412710:three_prime_UTR 25.0%
! TGTATAATGCTTCATGTATA+TGG - tig0023943:33875-33894 Msa1412710:CDS 25.0%
! TGTAACTATTGCTAAAATTG+TGG - tig0023943:34036-34055 Msa1412710:CDS 25.0%
! ATTTGAAGGAGTTAAAGAAT+CGG + tig0023943:34228-34247 None:intergenic 25.0%
!!! AAGTTGTTTTCATAATCACA+CGG + tig0023943:34318-34337 None:intergenic 25.0%
! GATTAAATTGTTTATGCAAC+TGG - tig0023943:34386-34405 Msa1412710:intron 25.0%
! TATTATGGTATGTCTTATGT+AGG - tig0023943:34445-34464 Msa1412710:intron 25.0%
! TGCATTATCAGAAGAATAAT+GGG - tig0023943:34812-34831 Msa1412710:intron 25.0%
! ATACATATTAGTTAGTTCGT+TGG + tig0023943:33441-33460 None:intergenic 25.0%
!!! TTGCTTTGAATTTAAGTTGA+AGG - tig0023943:33524-33543 Msa1412710:three_prime_UTR 25.0%
! TGTATAATGCTTCATGTATA+TGG - tig0023943:33875-33894 Msa1412710:CDS 25.0%
! TGTAACTATTGCTAAAATTG+TGG - tig0023943:34036-34055 Msa1412710:CDS 25.0%
! ATTTGAAGGAGTTAAAGAAT+CGG + tig0023943:34228-34247 None:intergenic 25.0%
!!! AAGTTGTTTTCATAATCACA+CGG + tig0023943:34318-34337 None:intergenic 25.0%
! GATTAAATTGTTTATGCAAC+TGG - tig0023943:34386-34405 Msa1412710:intron 25.0%
! TATTATGGTATGTCTTATGT+AGG - tig0023943:34445-34464 Msa1412710:intron 25.0%
! TGCATTATCAGAAGAATAAT+GGG - tig0023943:34812-34831 Msa1412710:intron 25.0%
CTAATACTTACCATCACATT+TGG - tig0023943:33555-33574 Msa1412710:three_prime_UTR 30.0%
! AATGGTTGTTATTGATAGAG+TGG + tig0023943:33586-33605 None:intergenic 30.0%
TTATATATGGCATAAGGCAA+TGG + tig0023943:33604-33623 None:intergenic 30.0%
TGCGTTAGATCAATAATGAA+TGG + tig0023943:33695-33714 None:intergenic 30.0%
TAATCATGTCACCAACAAAT+TGG - tig0023943:33754-33773 Msa1412710:CDS 30.0%
CATTATACATTACCTGATGA+TGG + tig0023943:33864-33883 None:intergenic 30.0%
CTCTAACTTAGTGTTATGTT+TGG - tig0023943:33955-33974 Msa1412710:CDS 30.0%
! CAATGAGTTTTGCAAAATCA+AGG - tig0023943:33983-34002 Msa1412710:CDS 30.0%
AACTATTGCTAAAATTGTGG+AGG - tig0023943:34039-34058 Msa1412710:CDS 30.0%
!! AGTTGTTTTCATAATCACAC+GGG + tig0023943:34317-34336 None:intergenic 30.0%
TATGTCTTATGTAGGTGTTA+AGG - tig0023943:34453-34472 Msa1412710:intron 30.0%
!!! TCCAGGAATTTTTTCAACAT+TGG + tig0023943:34593-34612 None:intergenic 30.0%
AGAATAGACTACAAACCTAA+TGG - tig0023943:34640-34659 Msa1412710:intron 30.0%
AAATCTTTGTGTTCCCAAAA+GGG - tig0023943:34785-34804 Msa1412710:intron 30.0%
GTGCATTATCAGAAGAATAA+TGG - tig0023943:34811-34830 Msa1412710:intron 30.0%
GCATTATCAGAAGAATAATG+GGG - tig0023943:34813-34832 Msa1412710:intron 30.0%
CTGTTTAGTATCAATCTGAA+AGG + tig0023943:34953-34972 None:intergenic 30.0%
CTTTCAGATTGATACTAAAC+AGG - tig0023943:34951-34970 Msa1412710:CDS 30.0%
!! TTGCATTTTGCTACTTGATA+TGG - tig0023943:35147-35166 Msa1412710:five_prime_UTR 30.0%
AATTCTATGAAGTAGCTAGA+AGG - tig0023943:35238-35257 Msa1412710:five_prime_UTR 30.0%
! CACATTTTATGTGGGATATA+TGG - tig0023943:35264-35283 Msa1412710:five_prime_UTR 30.0%
CTAATACTTACCATCACATT+TGG - tig0023943:33555-33574 Msa1412710:three_prime_UTR 30.0%
! AATGGTTGTTATTGATAGAG+TGG + tig0023943:33586-33605 None:intergenic 30.0%
TTATATATGGCATAAGGCAA+TGG + tig0023943:33604-33623 None:intergenic 30.0%
TGCGTTAGATCAATAATGAA+TGG + tig0023943:33695-33714 None:intergenic 30.0%
TAATCATGTCACCAACAAAT+TGG - tig0023943:33754-33773 Msa1412710:CDS 30.0%
CATTATACATTACCTGATGA+TGG + tig0023943:33864-33883 None:intergenic 30.0%
CTCTAACTTAGTGTTATGTT+TGG - tig0023943:33955-33974 Msa1412710:CDS 30.0%
! CAATGAGTTTTGCAAAATCA+AGG - tig0023943:33983-34002 Msa1412710:CDS 30.0%
AACTATTGCTAAAATTGTGG+AGG - tig0023943:34039-34058 Msa1412710:CDS 30.0%
!! AGTTGTTTTCATAATCACAC+GGG + tig0023943:34317-34336 None:intergenic 30.0%
TATGTCTTATGTAGGTGTTA+AGG - tig0023943:34453-34472 Msa1412710:intron 30.0%
!!! TCCAGGAATTTTTTCAACAT+TGG + tig0023943:34593-34612 None:intergenic 30.0%
AGAATAGACTACAAACCTAA+TGG - tig0023943:34640-34659 Msa1412710:intron 30.0%
AAATCTTTGTGTTCCCAAAA+GGG - tig0023943:34785-34804 Msa1412710:intron 30.0%
GTGCATTATCAGAAGAATAA+TGG - tig0023943:34811-34830 Msa1412710:intron 30.0%
GCATTATCAGAAGAATAATG+GGG - tig0023943:34813-34832 Msa1412710:intron 30.0%
CTGTTTAGTATCAATCTGAA+AGG + tig0023943:34953-34972 None:intergenic 30.0%
CTTTCAGATTGATACTAAAC+AGG - tig0023943:34951-34970 Msa1412710:CDS 30.0%
!! TTGCATTTTGCTACTTGATA+TGG - tig0023943:35147-35166 Msa1412710:five_prime_UTR 30.0%
AATTCTATGAAGTAGCTAGA+AGG - tig0023943:35238-35257 Msa1412710:five_prime_UTR 30.0%
! CACATTTTATGTGGGATATA+TGG - tig0023943:35264-35283 Msa1412710:five_prime_UTR 30.0%
ATTGACAGTCCAATGAAAAG+AGG + tig0023943:33399-33418 None:intergenic 35.0%
! GTTGTTATTGATAGAGTGGA+TGG + tig0023943:33582-33601 None:intergenic 35.0%
AAGAGAGAAGAAAGTTGAGT+TGG + tig0023943:33657-33676 None:intergenic 35.0%
! ATGAGAGTGCAAAAAACTCA+AGG + tig0023943:34086-34105 None:intergenic 35.0%
ATTCCCTTATGTACTCTTGA+GGG - tig0023943:34117-34136 Msa1412710:CDS 35.0%
TATAGAGTGCATAGTTCACT+CGG + tig0023943:34264-34283 None:intergenic 35.0%
TATGTAGGTGTTAAGGTGAA+TGG - tig0023943:34460-34479 Msa1412710:intron 35.0%
!!! GCAGCTGATTTTTTCTTTGA+TGG - tig0023943:34514-34533 Msa1412710:intron 35.0%
GCCAATGTTGAAAAAATTCC+TGG - tig0023943:34589-34608 Msa1412710:intron 35.0%
TTGGAAGGTGAATTAGATGT+AGG - tig0023943:34712-34731 Msa1412710:intron 35.0%
GAAATCTTTGTGTTCCCAAA+AGG - tig0023943:34784-34803 Msa1412710:intron 35.0%
! GAAACAATTGACAAAGTACC+AGG + tig0023943:34880-34899 None:intergenic 35.0%
!!! AGAAGGTGTCACATTTTATG+TGG - tig0023943:35255-35274 Msa1412710:five_prime_UTR 35.0%
!!! GAAGGTGTCACATTTTATGT+GGG - tig0023943:35256-35275 Msa1412710:five_prime_UTR 35.0%
ATTGACAGTCCAATGAAAAG+AGG + tig0023943:33399-33418 None:intergenic 35.0%
! GTTGTTATTGATAGAGTGGA+TGG + tig0023943:33582-33601 None:intergenic 35.0%
AAGAGAGAAGAAAGTTGAGT+TGG + tig0023943:33657-33676 None:intergenic 35.0%
! ATGAGAGTGCAAAAAACTCA+AGG + tig0023943:34086-34105 None:intergenic 35.0%
ATTCCCTTATGTACTCTTGA+GGG - tig0023943:34117-34136 Msa1412710:CDS 35.0%
TATAGAGTGCATAGTTCACT+CGG + tig0023943:34264-34283 None:intergenic 35.0%
TATGTAGGTGTTAAGGTGAA+TGG - tig0023943:34460-34479 Msa1412710:intron 35.0%
!!! GCAGCTGATTTTTTCTTTGA+TGG - tig0023943:34514-34533 Msa1412710:intron 35.0%
GCCAATGTTGAAAAAATTCC+TGG - tig0023943:34589-34608 Msa1412710:intron 35.0%
TTGGAAGGTGAATTAGATGT+AGG - tig0023943:34712-34731 Msa1412710:intron 35.0%
GAAATCTTTGTGTTCCCAAA+AGG - tig0023943:34784-34803 Msa1412710:intron 35.0%
! GAAACAATTGACAAAGTACC+AGG + tig0023943:34880-34899 None:intergenic 35.0%
!!! AGAAGGTGTCACATTTTATG+TGG - tig0023943:35255-35274 Msa1412710:five_prime_UTR 35.0%
!!! GAAGGTGTCACATTTTATGT+GGG - tig0023943:35256-35275 Msa1412710:five_prime_UTR 35.0%
CAGTCCAATGAAAAGAGGTT+TGG + tig0023943:33394-33413 None:intergenic 40.0%
! TAGTTAGTTCGTTGGTTGCT+GGG + tig0023943:33433-33452 None:intergenic 40.0%
! TTAGTTAGTTCGTTGGTTGC+TGG + tig0023943:33434-33453 None:intergenic 40.0%
!! GAGAAGAAAGTTGAGTTGGA+TGG + tig0023943:33653-33672 None:intergenic 40.0%
CAAAGGTCTTGAAGTGTATC+AGG + tig0023943:33831-33850 None:intergenic 40.0%
! ATGATGGAAGAGCTACACAA+AGG + tig0023943:33848-33867 None:intergenic 40.0%
GATTCCCTTATGTACTCTTG+AGG - tig0023943:34116-34135 Msa1412710:CDS 40.0%
! AGACCCTCAAGAGTACATAA+GGG + tig0023943:34123-34142 None:intergenic 40.0%
GTTGAGCTAAGGGAATTTGA+AGG + tig0023943:34242-34261 None:intergenic 40.0%
TGATGGTATAGCAAACCCTA+AGG - tig0023943:34531-34550 Msa1412710:intron 40.0%
! CAGTGTTGTTGACAACCTTA+GGG + tig0023943:34549-34568 None:intergenic 40.0%
! ACAGTGTTGTTGACAACCTT+AGG + tig0023943:34550-34569 None:intergenic 40.0%
AAGGTTGTCAACAACACTGT+AGG - tig0023943:34550-34569 Msa1412710:intron 40.0%
CAACAACACTGTAGGATCTT+TGG - tig0023943:34558-34577 Msa1412710:intron 40.0%
AATTCCTGGACTCAACACAT+TGG - tig0023943:34603-34622 Msa1412710:intron 40.0%
ATTCCTGGACTCAACACATT+GGG - tig0023943:34604-34623 Msa1412710:intron 40.0%
CAACACAAAAACAACCTCAG+TGG + tig0023943:34695-34714 None:intergenic 40.0%
!!! CACTGAGGTTGTTTTTGTGT+TGG - tig0023943:34693-34712 Msa1412710:intron 40.0%
!!! GAGGTTGTTTTTGTGTTGGA+AGG - tig0023943:34697-34716 Msa1412710:intron 40.0%
! GATAATGCACCAACCCTTTT+GGG + tig0023943:34801-34820 None:intergenic 40.0%
!! TGATAATGCACCAACCCTTT+TGG + tig0023943:34802-34821 None:intergenic 40.0%
! GCTTTTAACAGTCAACTTCC+TGG - tig0023943:34859-34878 Msa1412710:intron 40.0%
TCTGTTTGACTCTACACCAA+CGG - tig0023943:34903-34922 Msa1412710:CDS 40.0%
! GGTTTCTTTTCTTCTGTCTG+TGG - tig0023943:35285-35304 Msa1412710:five_prime_UTR 40.0%
!!! TGTGGTTATAGCTGCTTTTG+TGG - tig0023943:35303-35322 Msa1412710:five_prime_UTR 40.0%
!!! GTGGTTATAGCTGCTTTTGT+GGG - tig0023943:35304-35323 Msa1412710:five_prime_UTR 40.0%
TCCAATGAAAAGAGGTTTGG+TGG + tig0023943:33391-33410 None:intergenic 40.0%
CAGTCCAATGAAAAGAGGTT+TGG + tig0023943:33394-33413 None:intergenic 40.0%
! TAGTTAGTTCGTTGGTTGCT+GGG + tig0023943:33433-33452 None:intergenic 40.0%
! TTAGTTAGTTCGTTGGTTGC+TGG + tig0023943:33434-33453 None:intergenic 40.0%
!! GAGAAGAAAGTTGAGTTGGA+TGG + tig0023943:33653-33672 None:intergenic 40.0%
CAAAGGTCTTGAAGTGTATC+AGG + tig0023943:33831-33850 None:intergenic 40.0%
! ATGATGGAAGAGCTACACAA+AGG + tig0023943:33848-33867 None:intergenic 40.0%
GATTCCCTTATGTACTCTTG+AGG - tig0023943:34116-34135 Msa1412710:CDS 40.0%
! AGACCCTCAAGAGTACATAA+GGG + tig0023943:34123-34142 None:intergenic 40.0%
GTTGAGCTAAGGGAATTTGA+AGG + tig0023943:34242-34261 None:intergenic 40.0%
TGATGGTATAGCAAACCCTA+AGG - tig0023943:34531-34550 Msa1412710:intron 40.0%
! CAGTGTTGTTGACAACCTTA+GGG + tig0023943:34549-34568 None:intergenic 40.0%
! ACAGTGTTGTTGACAACCTT+AGG + tig0023943:34550-34569 None:intergenic 40.0%
AAGGTTGTCAACAACACTGT+AGG - tig0023943:34550-34569 Msa1412710:intron 40.0%
CAACAACACTGTAGGATCTT+TGG - tig0023943:34558-34577 Msa1412710:intron 40.0%
AATTCCTGGACTCAACACAT+TGG - tig0023943:34603-34622 Msa1412710:intron 40.0%
ATTCCTGGACTCAACACATT+GGG - tig0023943:34604-34623 Msa1412710:intron 40.0%
CAACACAAAAACAACCTCAG+TGG + tig0023943:34695-34714 None:intergenic 40.0%
!!! CACTGAGGTTGTTTTTGTGT+TGG - tig0023943:34693-34712 Msa1412710:intron 40.0%
!!! GAGGTTGTTTTTGTGTTGGA+AGG - tig0023943:34697-34716 Msa1412710:intron 40.0%
! GATAATGCACCAACCCTTTT+GGG + tig0023943:34801-34820 None:intergenic 40.0%
!! TGATAATGCACCAACCCTTT+TGG + tig0023943:34802-34821 None:intergenic 40.0%
! GCTTTTAACAGTCAACTTCC+TGG - tig0023943:34859-34878 Msa1412710:intron 40.0%
TCTGTTTGACTCTACACCAA+CGG - tig0023943:34903-34922 Msa1412710:CDS 40.0%
! GGTTTCTTTTCTTCTGTCTG+TGG - tig0023943:35285-35304 Msa1412710:five_prime_UTR 40.0%
!!! TGTGGTTATAGCTGCTTTTG+TGG - tig0023943:35303-35322 Msa1412710:five_prime_UTR 40.0%
!!! GTGGTTATAGCTGCTTTTGT+GGG - tig0023943:35304-35323 Msa1412710:five_prime_UTR 40.0%
AGTGGATGGACCAAATGTGA+TGG + tig0023943:33568-33587 None:intergenic 45.0%
AGAAGCGAAGGTAGATGAGA+TGG + tig0023943:33805-33824 None:intergenic 45.0%
TGTATCAGGATCAGAAGCGA+AGG + tig0023943:33817-33836 None:intergenic 45.0%
TGTGTAGCTCTTCCATCATC+AGG - tig0023943:33849-33868 Msa1412710:CDS 45.0%
! CAGACCCTCAAGAGTACATA+AGG + tig0023943:34124-34143 None:intergenic 45.0%
TGTACTCTTGAGGGTCTGTT+TGG - tig0023943:34126-34145 Msa1412710:CDS 45.0%
TCTTGAGGGTCTGTTTGGAT+TGG - tig0023943:34131-34150 Msa1412710:CDS 45.0%
AGTTCACTCGGTTGAGCTAA+GGG + tig0023943:34252-34271 None:intergenic 45.0%
TAGTTCACTCGGTTGAGCTA+AGG + tig0023943:34253-34272 None:intergenic 45.0%
! GGTGAATGGATTTGCATGCA+AGG - tig0023943:34474-34493 Msa1412710:intron 45.0%
CTTTGTGTTCCCAAAAGGGT+TGG - tig0023943:34789-34808 Msa1412710:intron 45.0%
AGAACATCATCAGGAACCGT+TGG + tig0023943:34922-34941 None:intergenic 45.0%
! TCCTGATGATGTTCTTGCTC+AGG - tig0023943:34927-34946 Msa1412710:CDS 45.0%
!!! GCTTTTGTGGGCATTGAACA+AGG - tig0023943:35316-35335 Msa1412710:five_prime_UTR 45.0%
AGTGGATGGACCAAATGTGA+TGG + tig0023943:33568-33587 None:intergenic 45.0%
AGAAGCGAAGGTAGATGAGA+TGG + tig0023943:33805-33824 None:intergenic 45.0%
TGTATCAGGATCAGAAGCGA+AGG + tig0023943:33817-33836 None:intergenic 45.0%
TGTGTAGCTCTTCCATCATC+AGG - tig0023943:33849-33868 Msa1412710:CDS 45.0%
! CAGACCCTCAAGAGTACATA+AGG + tig0023943:34124-34143 None:intergenic 45.0%
TGTACTCTTGAGGGTCTGTT+TGG - tig0023943:34126-34145 Msa1412710:CDS 45.0%
TCTTGAGGGTCTGTTTGGAT+TGG - tig0023943:34131-34150 Msa1412710:CDS 45.0%
AGTTCACTCGGTTGAGCTAA+GGG + tig0023943:34252-34271 None:intergenic 45.0%
TAGTTCACTCGGTTGAGCTA+AGG + tig0023943:34253-34272 None:intergenic 45.0%
! GGTGAATGGATTTGCATGCA+AGG - tig0023943:34474-34493 Msa1412710:intron 45.0%
CTTTGTGTTCCCAAAAGGGT+TGG - tig0023943:34789-34808 Msa1412710:intron 45.0%
AGAACATCATCAGGAACCGT+TGG + tig0023943:34922-34941 None:intergenic 45.0%
! TCCTGATGATGTTCTTGCTC+AGG - tig0023943:34927-34946 Msa1412710:CDS 45.0%
!!! GCTTTTGTGGGCATTGAACA+AGG - tig0023943:35316-35335 Msa1412710:five_prime_UTR 45.0%
ACTCCCAATGTGTTGAGTCC+AGG + tig0023943:34610-34629 None:intergenic 50.0%
!! TGTGGCGGGTTAAGACCATT+AGG + tig0023943:34658-34677 None:intergenic 50.0%
AAACAACCTCAGTGGCACGA+GGG + tig0023943:34687-34706 None:intergenic 50.0%
AAAACAACCTCAGTGGCACG+AGG + tig0023943:34688-34707 None:intergenic 50.0%
GCCTGAGCAAGAACATCATC+AGG + tig0023943:34931-34950 None:intergenic 50.0%
ACTCCCAATGTGTTGAGTCC+AGG + tig0023943:34610-34629 None:intergenic 50.0%
!! TGTGGCGGGTTAAGACCATT+AGG + tig0023943:34658-34677 None:intergenic 50.0%
AAACAACCTCAGTGGCACGA+GGG + tig0023943:34687-34706 None:intergenic 50.0%
AAAACAACCTCAGTGGCACG+AGG + tig0023943:34688-34707 None:intergenic 50.0%
GCCTGAGCAAGAACATCATC+AGG + tig0023943:34931-34950 None:intergenic 50.0%
! CACGAGGGTGTGTGTGTGGC+GGG + tig0023943:34672-34691 None:intergenic 65.0%
! GCACGAGGGTGTGTGTGTGG+CGG + tig0023943:34673-34692 None:intergenic 65.0%
! GTGGCACGAGGGTGTGTGTG+TGG + tig0023943:34676-34695 None:intergenic 65.0%
! CACACACCCTCGTGCCACTG+AGG - tig0023943:34678-34697 Msa1412710:intron 65.0%
! CACGAGGGTGTGTGTGTGGC+GGG + tig0023943:34672-34691 None:intergenic 65.0%
! GCACGAGGGTGTGTGTGTGG+CGG + tig0023943:34673-34692 None:intergenic 65.0%
! GTGGCACGAGGGTGTGTGTG+TGG + tig0023943:34676-34695 None:intergenic 65.0%
! CACACACCCTCGTGCCACTG+AGG - tig0023943:34678-34697 Msa1412710:intron 65.0%
Chromosome Type Strat End Strand Name
tig0023943 gene 33390 35361 33390 ID=Msa1412710;Name=Msa1412710
tig0023943 mRNA 33390 35361 33390 ID=Msa1412710-mRNA-1;Parent=Msa1412710;Name=Msa1412710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=369|1|1|1|1|1|2|347|219
tig0023943 exon 33390 34284 33390 ID=Msa1412710-mRNA-1:exon:20747;Parent=Msa1412710-mRNA-1
tig0023943 exon 34881 35361 34881 ID=Msa1412710-mRNA-1:exon:20746;Parent=Msa1412710-mRNA-1
tig0023943 five_prime_UTR 34993 35361 34993 ID=Msa1412710-mRNA-1:five_prime_utr;Parent=Msa1412710-mRNA-1
tig0023943 CDS 34881 34992 34881 ID=Msa1412710-mRNA-1:cds;Parent=Msa1412710-mRNA-1
tig0023943 CDS 33737 34284 33737 ID=Msa1412710-mRNA-1:cds;Parent=Msa1412710-mRNA-1
tig0023943 three_prime_UTR 33390 33736 33390 ID=Msa1412710-mRNA-1:three_prime_utr;Parent=Msa1412710-mRNA-1
Gene Sequence

>Msa1412710

ATGTCACCAACAAATTGGACAATTTTTTTCATTTTGTGCTGTGCCATCTCATCTACCTTCGCTTCTGATCCTGATACACTTCAAGACCTTTGTGTAGCTCTTCCATCATCAGGTGTTAAGGTGAATGGATTTGCATGCAAGGCAGAGTCAAATGTAACAGCAGCTGATTTTTTCTTTGATGGTATAGCAAACCCTAAGGTTGTCAACAACACTGTAGGATCTTTGGTTACTGCAGCCAATGTTGAAAAAATTCCTGGACTCAACACATTGGGAGTATCTTTATCAAGAATAGACTACAAACCTAATGGTCTTAACCCGCCACACACACACCCTCGTGCCACTGAGGTTGTTTTTGTGTTGGAAGGTGAATTAGATGTAGGTTTTATCACTACATCAAACAAACTCATTTCTAAGTCCATAAAAAAAGGTGAAATCTTTGTGTTCCCAAAAGGGTTGGTGCATTATCAGAAGAATAATGGGGATAAAGCTGCTTCTGTGATTTCTGCTTTTAACAGTCAACTTCCTGGTACTTTGTCAATTGTTTCATCTCTGTTTGACTCTACACCAACGGTTCCTGATGATGTTCTTGCTCAGGCCTTTCAGATTGATACTAAACAGGTTGATGAAATAAAGACAAAGATTGCTCCTAAGAAGACTTAA
Protein sequence

>Msa1412710

MSPTNWTIFFILCCAISSTFASDPDTLQDLCVALPSSGVKVNGFACKAESNVTAADFFFDGIANPKVVNNTVGSLVTAANVEKIPGLNTLGVSLSRIDYKPNGLNPPHTHPRATEVVFVLEGELDVGFITTSNKLISKSIKKGEIFVFPKGLVHYQKNNGDKAASVISAFNSQLPGTLSIVSSLFDSTPTVPDDVLAQAFQIDTKQVDEIKTKIAPKKT*