AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0945080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0945080 MtrunA17_Chr4g0000411 96.369 358 11 1 1 358 1 356 0.0 694
Msa0945080 MtrunA17_Chr4g0002141 64.305 367 69 5 1 358 1 314 7.87e-154 440
Msa0945080 MtrunA17_Chr2g0289421 43.251 363 144 11 1 358 1 306 5.20e-78 246
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0945080 AT1G16860.1 71.625 363 87 6 1 358 1 352 5.31e-180 508
Msa0945080 AT1G78880.1 70.799 363 84 7 1 358 1 346 5.79e-176 498
Msa0945080 AT4G22290.1 43.370 362 162 10 1 358 1 323 7.15e-75 239

Find 117 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTATTCAGTTGCTTCTTT+TGG 0.131393 6_3:-23653640 Msa0945080:five_prime_UTR
GGACAGTTTGTGAAGGTTTC+TGG 0.153440 6_3:-23652742 Msa0945080:CDS
TTCTATCTTCTTCATTCTTC+AGG 0.199375 6_3:-23654511 Msa0945080:intron
CTTGAAGGAGCGTTATTTAA+TGG 0.218734 6_3:+23653399 None:intergenic
ATCAAGAAGTCAGGAGAATT+AGG 0.233497 6_3:-23653471 Msa0945080:CDS
GTCCTTTCTTTCGGCTGTTC+AGG 0.252687 6_3:+23653537 None:intergenic
CTTCACAAACTGTCCATTCT+TGG 0.261830 6_3:+23652750 None:intergenic
GGGGCTCCAAGGAAGGTTTC+TGG 0.291580 6_3:-23653096 Msa0945080:CDS
TTGATGTCACCGCCGGTATA+AGG 0.304998 6_3:+23653489 None:intergenic
TCAAGGACTGGATCGTTTGC+AGG 0.327688 6_3:-23653381 Msa0945080:CDS
TCTGGCCCGCTGAATTCATC+TGG 0.342002 6_3:-23653117 Msa0945080:CDS
GGTTCCAAATCTAGAAAATC+TGG 0.360653 6_3:-23653423 Msa0945080:CDS
AAACCAGTTGTTGGAAGAAC+TGG 0.362727 6_3:+23653147 None:intergenic
TTCGTTATGGGATTCATTGC+TGG 0.376875 6_3:-23652931 Msa0945080:CDS
ACAGACCACAATATTGGCTT+TGG 0.378088 6_3:+23652964 None:intergenic
GAGGTATTGGACCCGAGTTT+TGG 0.382636 6_3:+23653169 None:intergenic
GTTGGAAGAACTGGAGGTAT+TGG 0.387217 6_3:+23653156 None:intergenic
TCTGGTCCATTAGAGTCCTT+AGG 0.387635 6_3:-23653078 Msa0945080:CDS
ATTGACCCTAAGGACTCTAA+TGG 0.391975 6_3:+23653072 None:intergenic
TACTACAACAATAAGGAGTA+TGG 0.395337 6_3:+23652879 None:intergenic
AGAATCACAGACCACAATAT+TGG 0.401174 6_3:+23652958 None:intergenic
ATTGTTGGAGTCCTTTCTTT+CGG 0.401281 6_3:+23653528 None:intergenic
CCTCCAGTTCTTCCAACAAC+TGG 0.402248 6_3:-23653150 Msa0945080:CDS
TGCGACTGCTTGAAATACTT+TGG 0.406894 6_3:-23652253 Msa0945080:CDS
GACAGTTTGTGAAGGTTTCT+GGG 0.407064 6_3:-23652741 Msa0945080:CDS
GATGTAATCAAACCAGTTGT+TGG 0.409587 6_3:+23653138 None:intergenic
GTTGAATAAACACGGGGAAC+CGG 0.414266 6_3:-23653232 Msa0945080:CDS
GCAAAACGCAGCAGCTCGTT+CGG 0.414432 6_3:-23653331 Msa0945080:CDS
AAAGAATTACTACAACAATA+AGG 0.422326 6_3:+23652872 None:intergenic
ACAACTGGTTTGATTACATC+TGG 0.434300 6_3:-23653135 Msa0945080:CDS
GCTGTCCTCAGCTCTGCATC+AGG 0.437236 6_3:+23652772 None:intergenic
CGGTGGCGGCATTATTCACT+TGG 0.440693 6_3:-23652843 Msa0945080:CDS
GGACCAGAGGACTTCTTAAC+CGG 0.441130 6_3:+23653213 None:intergenic
TGGATGATGAATATTCCTTC+AGG 0.443248 6_3:-23652996 Msa0945080:CDS
CTGGTCCATTAGAGTCCTTA+GGG 0.448812 6_3:-23653077 Msa0945080:CDS
AAGTCCTCTGGTCCTCAATC+AGG 0.449552 6_3:-23653204 Msa0945080:CDS
CAGACCACAATATTGGCTTT+GGG 0.455761 6_3:+23652965 None:intergenic
TCAAGAAGTCAGGAGAATTA+GGG 0.462102 6_3:-23653470 Msa0945080:CDS
AACGAAGTCCCCAGGTAAAA+CGG 0.463388 6_3:+23652351 None:intergenic
GCAAACGATCCAGTCCTTGA+AGG 0.463630 6_3:+23653384 None:intergenic
CTGGCCCGCTGAATTCATCT+GGG 0.463840 6_3:-23653116 Msa0945080:CDS
AAAACAGGTGCTGCAACAAC+TGG 0.464653 6_3:+23654758 None:intergenic
GCAGGAGCTGCTTCGCACTC+TGG 0.466667 6_3:-23653363 Msa0945080:CDS
CGTGGAAGGAAAGCTATTGT+TGG 0.472794 6_3:-23652811 Msa0945080:CDS
CATTAAATAACGCTCCTTCA+AGG 0.473559 6_3:-23653398 Msa0945080:CDS
TGTCGAATTCAAATAAAACT+TGG 0.477026 6_3:+23652282 None:intergenic
TGGTGGCACACGCCAAAACT+CGG 0.478089 6_3:-23653181 Msa0945080:CDS
TGAGAGAAAGAGAAAGACAA+TGG 0.479606 6_3:-23654577 Msa0945080:five_prime_UTR
CGTTCGGCTTATGTCACATC+AGG 0.483108 6_3:-23653315 Msa0945080:CDS
ACTAGCTTGTATGAATATAG+AGG 0.487035 6_3:-23652407 Msa0945080:CDS
ATTTGATTTCATTGACCCTA+AGG 0.487683 6_3:+23653062 None:intergenic
AATGGACCAGAAACCTTCCT+TGG 0.496121 6_3:+23653090 None:intergenic
CTACTTCAGCCTCAATGAAA+AGG 0.499132 6_3:-23653269 Msa0945080:CDS
CTAGATTTGGAACCATCCAT+AGG 0.500838 6_3:+23653432 None:intergenic
ATGGCAACTGAGCTCATTGT+TGG 0.505538 6_3:+23653513 None:intergenic
GGCGGTGACATCAAGAAGTC+AGG 0.506595 6_3:-23653480 Msa0945080:CDS
GTGGATGTATATACACATCT+TGG 0.506836 6_3:+23652428 None:intergenic
CTTTGGGAAGTTCTTCCTGA+AGG 0.511332 6_3:+23652981 None:intergenic
TCAGGTAATCTATCTGCTGC+AGG 0.515107 6_3:-23653297 Msa0945080:CDS
GTCCTGAACAGCCGAAAGAA+AGG 0.519111 6_3:-23653539 Msa0945080:CDS
TCTGGTCCTCAATCAGGTGG+TGG 0.523811 6_3:-23653198 Msa0945080:CDS
TGCTTGAAATACTTTGGCCA+TGG 0.524319 6_3:-23652247 Msa0945080:CDS
GAGAGAAAGAGAAAGACAAT+GGG 0.524985 6_3:-23654576 Msa0945080:five_prime_UTR
GAGCTGAGGACAGCCAAGAA+TGG 0.527974 6_3:-23652763 Msa0945080:CDS
AGCAATGGCCTGTATGTATC+GGG 0.528003 6_3:-23653564 Msa0945080:CDS
TCAGTTGCCATGCCTTATAC+CGG 0.528246 6_3:-23653501 Msa0945080:CDS
GAACCGGTTAAGAAGTCCTC+TGG 0.529575 6_3:-23653216 Msa0945080:CDS
CCATTGCTTAGCTGATGAGA+TGG 0.532954 6_3:+23653579 None:intergenic
ATCAGCGAAGCGAACTAGTA+CGG 0.537951 6_3:-23653665 Msa0945080:five_prime_UTR
AAGCAATGGCCTGTATGTAT+CGG 0.538649 6_3:-23653565 Msa0945080:CDS
AATAACGCTCCTTCAAGGAC+TGG 0.539760 6_3:-23653393 Msa0945080:CDS
TTCATCTGGGGCTCCAAGGA+AGG 0.539779 6_3:-23653103 Msa0945080:CDS
GAGCCCCAGATGAATTCAGC+GGG 0.540465 6_3:+23653112 None:intergenic
ATTTGAAAAGATATCAGTGA+TGG 0.540525 6_3:-23653613 Msa0945080:exon
GCTTGTATGAATATAGAGGA+TGG 0.540592 6_3:-23652403 Msa0945080:CDS
AGATATCAGTGATGGGTTCG+AGG 0.547275 6_3:-23653605 Msa0945080:exon
AAATATATAATTCACATCCA+TGG 0.550350 6_3:+23652230 None:intergenic
CTTGGAATAGTTGTCGTGGA+AGG 0.550581 6_3:-23652825 Msa0945080:CDS
TAGATTTGGAACCATCCATA+GGG 0.554139 6_3:+23653433 None:intergenic
CAGGACGGCCCGATACATAC+AGG 0.557100 6_3:+23653556 None:intergenic
TCTATATTCATACAAGCTAG+TGG 0.559080 6_3:+23652409 None:intergenic
CTCAGTTGAACGAAGTCCCC+AGG 0.559128 6_3:+23652343 None:intergenic
GAAAATGTTTGATATCCCTA+TGG 0.559261 6_3:-23653448 Msa0945080:CDS
TGAATTCATCTGGGGCTCCA+AGG 0.561974 6_3:-23653107 Msa0945080:CDS
TCCTCTGGTCCTCAATCAGG+TGG 0.562994 6_3:-23653201 Msa0945080:CDS
CATTGCTTAGCTGATGAGAT+GGG 0.563787 6_3:+23653580 None:intergenic
ACACTGAGAGTAGTCACAGC+TGG 0.564624 6_3:+23653018 None:intergenic
ATGTTTGATATCCCTATGGA+TGG 0.566234 6_3:-23653444 Msa0945080:CDS
CAAGAATGGACAGTTTGTGA+AGG 0.570520 6_3:-23652749 Msa0945080:CDS
TTTCTTTCGGCTGTTCAGGA+CGG 0.577953 6_3:+23653541 None:intergenic
CTTGTATGAATATAGAGGAT+GGG 0.582610 6_3:-23652402 Msa0945080:CDS
GGTGGCACACGCCAAAACTC+GGG 0.583014 6_3:-23653180 Msa0945080:CDS
CTCTAATGGAACATTGCCGC+AGG 0.583097 6_3:+23652469 None:intergenic
TTCACTTGGAATAGTTGTCG+TGG 0.586701 6_3:-23652829 Msa0945080:CDS
GGTTCCCCGTGTTTATTCAA+CGG 0.589761 6_3:+23653234 None:intergenic
TGACTTCTTGATGTCACCGC+CGG 0.591147 6_3:+23653482 None:intergenic
GTTATGGGATTCATTGCTGG+AGG 0.597123 6_3:-23652928 Msa0945080:CDS
TTTGAAAAGATATCAGTGAT+GGG 0.606674 6_3:-23653612 Msa0945080:exon
TCAATGAAAAGGACAAACTC+AGG 0.614891 6_3:-23653258 Msa0945080:CDS
GTCACCGCCGGTATAAGGCA+TGG 0.615654 6_3:+23653494 None:intergenic
GGAGCCCCAGATGAATTCAG+CGG 0.619447 6_3:+23653111 None:intergenic
CCATCTCATCAGCTAAGCAA+TGG 0.621590 6_3:-23653579 Msa0945080:CDS
CAGGTCCGTTGAATAAACAC+GGG 0.631654 6_3:-23653239 Msa0945080:CDS
CCAGTTGTTGGAAGAACTGG+AGG 0.633647 6_3:+23653150 None:intergenic
ACCACCTGATTGAGGACCAG+AGG 0.634928 6_3:+23653200 None:intergenic
ACAGTTTGTGAAGGTTTCTG+GGG 0.638708 6_3:-23652740 Msa0945080:intron
CAGGTGCTGCAACAACTGGT+AGG 0.646360 6_3:+23654762 None:intergenic
TCAGGTCCGTTGAATAAACA+CGG 0.649376 6_3:-23653240 Msa0945080:CDS
AATATCCTGATGCAGAGCTG+AGG 0.654877 6_3:-23652777 Msa0945080:CDS
TGGCCCGCTGAATTCATCTG+GGG 0.657399 6_3:-23653115 Msa0945080:CDS
GTTGCCATGCCTTATACCGG+CGG 0.664295 6_3:-23653498 Msa0945080:CDS
CTGGGGACTTCGTTCAACTG+AGG 0.664974 6_3:-23652342 Msa0945080:CDS
ACTTCCCAAAGCCAATATTG+TGG 0.667244 6_3:-23652969 Msa0945080:CDS
CAATAAGGAGTATGGCATTG+CGG 0.670570 6_3:+23652887 None:intergenic
CGTGTGCCACCACCTGATTG+AGG 0.677788 6_3:+23653192 None:intergenic
AGCTGTGACTACTCTCAGTG+TGG 0.690771 6_3:-23653016 Msa0945080:CDS
AGGTCCGTTGAATAAACACG+GGG 0.753684 6_3:-23653238 Msa0945080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTTTTATGTAATTAT+TGG - chr6_3:23652978-23652997 Msa0945080:CDS 10.0%
!!! AAAAATAGATTTTTCGAAAT+GGG + chr6_3:23652658-23652677 None:intergenic 15.0%
!!! AAAAAATAGATTTTTCGAAA+TGG + chr6_3:23652659-23652678 None:intergenic 15.0%
!!! ATTTATCAGATTATGACTTT+TGG - chr6_3:23652690-23652709 Msa0945080:intron 20.0%
!!! TTTATCAGATTATGACTTTT+GGG - chr6_3:23652691-23652710 Msa0945080:intron 20.0%
!! ACTCAATAATGATATATGAA+TGG - chr6_3:23652773-23652792 Msa0945080:CDS 20.0%
!!! CTATCTAACTAATTTTACTT+TGG - chr6_3:23652866-23652885 Msa0945080:CDS 20.0%
!! TAAAAACAAAAACTATGTAG+TGG + chr6_3:23652969-23652988 None:intergenic 20.0%
!!! GTAATTAGTTTATCTTATCT+TGG - chr6_3:23653090-23653109 Msa0945080:CDS 20.0%
!! AATTATCATAATAACAATGC+TGG + chr6_3:23653140-23653159 None:intergenic 20.0%
!!! ATTCTGTTTCAATCTTTTTT+AGG - chr6_3:23653292-23653311 Msa0945080:CDS 20.0%
!! AAAGAATTACTACAACAATA+AGG + chr6_3:23654145-23654164 None:intergenic 20.0%
!! CAAAAGTTACAATATATCAA+TGG + chr6_3:23654515-23654534 None:intergenic 20.0%
!!! TGATATATTGTAACTTTTGA+AGG - chr6_3:23654516-23654535 Msa0945080:five_prime_UTR 20.0%
! TAGAATAGAATCAAAAAGAG+TGG + chr6_3:23652488-23652507 None:intergenic 25.0%
!! TTATCAGATTATGACTTTTG+GGG - chr6_3:23652692-23652711 Msa0945080:intron 25.0%
! TTGCACATTGTAATAAAACT+TGG - chr6_3:23652723-23652742 Msa0945080:intron 25.0%
! TGCACATTGTAATAAAACTT+GGG - chr6_3:23652724-23652743 Msa0945080:intron 25.0%
!! CTAACTAATTTTACTTTGGA+TGG - chr6_3:23652870-23652889 Msa0945080:CDS 25.0%
! GTAAAGTAAGAATGAAGAAA+AGG + chr6_3:23652916-23652935 None:intergenic 25.0%
! GAAAGGGTAAATTAAAATGA+TGG + chr6_3:23652938-23652957 None:intergenic 25.0%
! TTCATCATAGTTATAGTCTA+AGG - chr6_3:23653188-23653207 Msa0945080:CDS 25.0%
! ATTTGAAAAGATATCAGTGA+TGG - chr6_3:23653401-23653420 Msa0945080:CDS 25.0%
! TTTGAAAAGATATCAGTGAT+GGG - chr6_3:23653402-23653421 Msa0945080:CDS 25.0%
! TTCAAAACATGAATGATTGA+TGG - chr6_3:23654482-23654501 Msa0945080:intron 25.0%
!!! TATATTGTAACTTTTGAAGG+TGG - chr6_3:23654519-23654538 Msa0945080:five_prime_UTR 25.0%
! TGTCGAATTCAAATAAAACT+TGG + chr6_3:23654735-23654754 None:intergenic 25.0%
!!! ACCTGTTTTTTTAAGAGAGA+GGG - chr6_3:23652270-23652289 Msa0945080:CDS 30.0%
AGAGAGAGAAAAGAAAAAAG+AGG - chr6_3:23652383-23652402 Msa0945080:CDS 30.0%
TTCTATCTTCTTCATTCTTC+AGG - chr6_3:23652503-23652522 Msa0945080:intron 30.0%
TTGCATTCATACTCTTCAAA+TGG - chr6_3:23652626-23652645 Msa0945080:intron 30.0%
TGCATTCATACTCTTCAAAT+GGG - chr6_3:23652627-23652646 Msa0945080:intron 30.0%
AGAGAAATTTGGAAGTGAAA+TGG + chr6_3:23652831-23652850 None:intergenic 30.0%
ATCTAGAGGAAAGAGAAATT+TGG + chr6_3:23652842-23652861 None:intergenic 30.0%
AGTTAGATAGATAGATCTAG+AGG + chr6_3:23652856-23652875 None:intergenic 30.0%
TGTAGTGGTGTTTAAAGAAA+GGG + chr6_3:23652954-23652973 None:intergenic 30.0%
ATGTAGTGGTGTTTAAAGAA+AGG + chr6_3:23652955-23652974 None:intergenic 30.0%
! AATAACAATGCTGGATTTTC+TGG + chr6_3:23653131-23653150 None:intergenic 30.0%
!!! CTGTTTCAATCTTTTTTAGG+TGG - chr6_3:23653295-23653314 Msa0945080:CDS 30.0%
! TCTTATTCAGTTGCTTCTTT+TGG - chr6_3:23653374-23653393 Msa0945080:CDS 30.0%
! CTTATTCAGTTGCTTCTTTT+GGG - chr6_3:23653375-23653394 Msa0945080:CDS 30.0%
GAAAATGTTTGATATCCCTA+TGG - chr6_3:23653566-23653585 Msa0945080:CDS 30.0%
! ATTTGATTTCATTGACCCTA+AGG + chr6_3:23653955-23653974 None:intergenic 30.0%
! TGTGATTCTGATTTTCGTTA+TGG - chr6_3:23654070-23654089 Msa0945080:intron 30.0%
! GTGATTCTGATTTTCGTTAT+GGG - chr6_3:23654071-23654090 Msa0945080:intron 30.0%
TACTACAACAATAAGGAGTA+TGG + chr6_3:23654138-23654157 None:intergenic 30.0%
! TGTAGTAATTCTTTTTGCTG+CGG - chr6_3:23654151-23654170 Msa0945080:intron 30.0%
AAATACAGTCAAACAATGTG+TGG + chr6_3:23654388-23654407 None:intergenic 30.0%
! GTATTTTTCTCATTCATCCA+TGG - chr6_3:23654402-23654421 Msa0945080:intron 30.0%
! ACATCTTGGAACTTTTTGAA+AGG + chr6_3:23654575-23654594 None:intergenic 30.0%
TCTATATTCATACAAGCTAG+TGG + chr6_3:23654608-23654627 None:intergenic 30.0%
! ACTAGCTTGTATGAATATAG+AGG - chr6_3:23654607-23654626 Msa0945080:five_prime_UTR 30.0%
CTTGTATGAATATAGAGGAT+GGG - chr6_3:23654612-23654631 Msa0945080:five_prime_UTR 30.0%
!!! CACCTGTTTTTTTAAGAGAG+AGG - chr6_3:23652269-23652288 Msa0945080:CDS 35.0%
!!! CCTGTTTTTTTAAGAGAGAG+GGG - chr6_3:23652271-23652290 Msa0945080:CDS 35.0%
!!! CTGTTTTTTTAAGAGAGAGG+GGG - chr6_3:23652272-23652291 Msa0945080:CDS 35.0%
TGAGAGAAAGAGAAAGACAA+TGG - chr6_3:23652437-23652456 Msa0945080:CDS 35.0%
GAGAGAAAGAGAAAGACAAT+GGG - chr6_3:23652438-23652457 Msa0945080:CDS 35.0%
AGCGAAGATCTGAGAAAAAT+GGG + chr6_3:23652604-23652623 None:intergenic 35.0%
GCATTCATACTCTTCAAATG+GGG - chr6_3:23652628-23652647 Msa0945080:intron 35.0%
CATTCATACTCTTCAAATGG+GGG - chr6_3:23652629-23652648 Msa0945080:intron 35.0%
! CGCAACATGTTTACATTTTG+AGG - chr6_3:23652800-23652819 Msa0945080:CDS 35.0%
!! TTTTAGGTGGCATTGTTTTG+CGG - chr6_3:23653308-23653327 Msa0945080:CDS 35.0%
ATTGTTGGAGTCCTTTCTTT+CGG + chr6_3:23653489-23653508 None:intergenic 35.0%
ATCAAGAAGTCAGGAGAATT+AGG - chr6_3:23653543-23653562 Msa0945080:CDS 35.0%
TCAAGAAGTCAGGAGAATTA+GGG - chr6_3:23653544-23653563 Msa0945080:CDS 35.0%
ATGTTTGATATCCCTATGGA+TGG - chr6_3:23653570-23653589 Msa0945080:CDS 35.0%
! TAGATTTGGAACCATCCATA+GGG + chr6_3:23653584-23653603 None:intergenic 35.0%
GGTTCCAAATCTAGAAAATC+TGG - chr6_3:23653591-23653610 Msa0945080:CDS 35.0%
! TGGACCAGATTTTCTAGATT+TGG + chr6_3:23653598-23653617 None:intergenic 35.0%
!! CTTGAAGGAGCGTTATTTAA+TGG + chr6_3:23653618-23653637 None:intergenic 35.0%
CATTAAATAACGCTCCTTCA+AGG - chr6_3:23653616-23653635 Msa0945080:exon 35.0%
! TGAGTTTGTCCTTTTCATTG+AGG + chr6_3:23653757-23653776 None:intergenic 35.0%
TCAATGAAAAGGACAAACTC+AGG - chr6_3:23653756-23653775 Msa0945080:intron 35.0%
GATGTAATCAAACCAGTTGT+TGG + chr6_3:23653879-23653898 None:intergenic 35.0%
!! ACAACTGGTTTGATTACATC+TGG - chr6_3:23653879-23653898 Msa0945080:intron 35.0%
TGGATGATGAATATTCCTTC+AGG - chr6_3:23654018-23654037 Msa0945080:intron 35.0%
AGAATCACAGACCACAATAT+TGG + chr6_3:23654059-23654078 None:intergenic 35.0%
!!! ATTGCTGGAGGTTTTATTCT+TGG - chr6_3:23654098-23654117 Msa0945080:intron 35.0%
! AAACAATGTGTGGAAAGTGT+TGG + chr6_3:23654378-23654397 None:intergenic 35.0%
CCACTTTATGAACTAATCCA+TGG + chr6_3:23654422-23654441 None:intergenic 35.0%
!! CCATGGATTAGTTCATAAAG+TGG - chr6_3:23654419-23654438 Msa0945080:intron 35.0%
!! TTTTGAAAGGAGGACTCTAA+TGG + chr6_3:23654562-23654581 None:intergenic 35.0%
!! TCTTGGAACTTTTTGAAAGG+AGG + chr6_3:23654572-23654591 None:intergenic 35.0%
GTGGATGTATATACACATCT+TGG + chr6_3:23654589-23654608 None:intergenic 35.0%
GCTTGTATGAATATAGAGGA+TGG - chr6_3:23654611-23654630 Msa0945080:five_prime_UTR 35.0%
CCCCTCTCTCTTAAAAAAAC+AGG + chr6_3:23652274-23652293 None:intergenic 40.0%
AGAATCAAAAAGAGTGGCGA+TGG + chr6_3:23652482-23652501 None:intergenic 40.0%
GAGCGAAGATCTGAGAAAAA+TGG + chr6_3:23652605-23652624 None:intergenic 40.0%
!! CTGGATTTTCTGGTACATGA+TGG + chr6_3:23653121-23653140 None:intergenic 40.0%
CATTGCTTAGCTGATGAGAT+GGG + chr6_3:23653437-23653456 None:intergenic 40.0%
AAGCAATGGCCTGTATGTAT+CGG - chr6_3:23653449-23653468 Msa0945080:CDS 40.0%
! CTAGATTTGGAACCATCCAT+AGG + chr6_3:23653585-23653604 None:intergenic 40.0%
CTACTTCAGCCTCAATGAAA+AGG - chr6_3:23653745-23653764 Msa0945080:intron 40.0%
TCAGGTCCGTTGAATAAACA+CGG - chr6_3:23653774-23653793 Msa0945080:intron 40.0%
AAACCAGTTGTTGGAAGAAC+TGG + chr6_3:23653870-23653889 None:intergenic 40.0%
ATTGACCCTAAGGACTCTAA+TGG + chr6_3:23653945-23653964 None:intergenic 40.0%
ACTTCCCAAAGCCAATATTG+TGG - chr6_3:23654045-23654064 Msa0945080:intron 40.0%
!! CAGACCACAATATTGGCTTT+GGG + chr6_3:23654052-23654071 None:intergenic 40.0%
!! ACAGACCACAATATTGGCTT+TGG + chr6_3:23654053-23654072 None:intergenic 40.0%
TTCGTTATGGGATTCATTGC+TGG - chr6_3:23654083-23654102 Msa0945080:intron 40.0%
! CAATAAGGAGTATGGCATTG+CGG + chr6_3:23654130-23654149 None:intergenic 40.0%
! AGTAATTCTTTTTGCTGCGG+TGG - chr6_3:23654154-23654173 Msa0945080:intron 40.0%
TTCACTTGGAATAGTTGTCG+TGG - chr6_3:23654185-23654204 Msa0945080:intron 40.0%
CTTCACAAACTGTCCATTCT+TGG + chr6_3:23654267-23654286 None:intergenic 40.0%
CAAGAATGGACAGTTTGTGA+AGG - chr6_3:23654265-23654284 Msa0945080:intron 40.0%
GACAGTTTGTGAAGGTTTCT+GGG - chr6_3:23654273-23654292 Msa0945080:intron 40.0%
ACAGTTTGTGAAGGTTTCTG+GGG - chr6_3:23654274-23654293 Msa0945080:intron 40.0%
GTGTTGGATGTGTGTGTATT+TGG + chr6_3:23654362-23654381 None:intergenic 40.0%
!! TAAACATCGCCGTTTTACCT+GGG - chr6_3:23654654-23654673 Msa0945080:five_prime_UTR 40.0%
TGCGACTGCTTGAAATACTT+TGG - chr6_3:23654761-23654780 Msa0945080:five_prime_UTR 40.0%
TGCTTGAAATACTTTGGCCA+TGG - chr6_3:23654767-23654786 Msa0945080:five_prime_UTR 40.0%
! AAAACAGGTGCTGCAACAAC+TGG + chr6_3:23652259-23652278 None:intergenic 45.0%
ATCAGCGAAGCGAACTAGTA+CGG - chr6_3:23653349-23653368 Msa0945080:CDS 45.0%
!! AGATATCAGTGATGGGTTCG+AGG - chr6_3:23653409-23653428 Msa0945080:CDS 45.0%
CCATTGCTTAGCTGATGAGA+TGG + chr6_3:23653438-23653457 None:intergenic 45.0%
CCATCTCATCAGCTAAGCAA+TGG - chr6_3:23653435-23653454 Msa0945080:CDS 45.0%
AGCAATGGCCTGTATGTATC+GGG - chr6_3:23653450-23653469 Msa0945080:CDS 45.0%
TTTCTTTCGGCTGTTCAGGA+CGG + chr6_3:23653476-23653495 None:intergenic 45.0%
ATGGCAACTGAGCTCATTGT+TGG + chr6_3:23653504-23653523 None:intergenic 45.0%
TCAGTTGCCATGCCTTATAC+CGG - chr6_3:23653513-23653532 Msa0945080:CDS 45.0%
AATAACGCTCCTTCAAGGAC+TGG - chr6_3:23653621-23653640 Msa0945080:five_prime_UTR 45.0%
! GCTGCTGCGTTTTGCATAAT+AGG + chr6_3:23653678-23653697 None:intergenic 45.0%
TCAGGTAATCTATCTGCTGC+AGG - chr6_3:23653717-23653736 Msa0945080:intron 45.0%
CAGGTCCGTTGAATAAACAC+GGG - chr6_3:23653775-23653794 Msa0945080:intron 45.0%
AGGTCCGTTGAATAAACACG+GGG - chr6_3:23653776-23653795 Msa0945080:intron 45.0%
GGTTCCCCGTGTTTATTCAA+CGG + chr6_3:23653783-23653802 None:intergenic 45.0%
GTTGAATAAACACGGGGAAC+CGG - chr6_3:23653782-23653801 Msa0945080:intron 45.0%
! GTTGGAAGAACTGGAGGTAT+TGG + chr6_3:23653861-23653880 None:intergenic 45.0%
AATGGACCAGAAACCTTCCT+TGG + chr6_3:23653927-23653946 None:intergenic 45.0%
TCTGGTCCATTAGAGTCCTT+AGG - chr6_3:23653936-23653955 Msa0945080:intron 45.0%
CTGGTCCATTAGAGTCCTTA+GGG - chr6_3:23653937-23653956 Msa0945080:intron 45.0%
! CTTTGGGAAGTTCTTCCTGA+AGG + chr6_3:23654036-23654055 None:intergenic 45.0%
GTTATGGGATTCATTGCTGG+AGG - chr6_3:23654086-23654105 Msa0945080:intron 45.0%
! AATTCTTTTTGCTGCGGTGG+CGG - chr6_3:23654157-23654176 Msa0945080:intron 45.0%
CTTGGAATAGTTGTCGTGGA+AGG - chr6_3:23654189-23654208 Msa0945080:intron 45.0%
CGTGGAAGGAAAGCTATTGT+TGG - chr6_3:23654203-23654222 Msa0945080:intron 45.0%
AATATCCTGATGCAGAGCTG+AGG - chr6_3:23654237-23654256 Msa0945080:intron 45.0%
GGACAGTTTGTGAAGGTTTC+TGG - chr6_3:23654272-23654291 Msa0945080:intron 45.0%
!! CTTTTGAAGGTGGTAACCTG+CGG - chr6_3:23654529-23654548 Msa0945080:five_prime_UTR 45.0%
!! CTAAACATCGCCGTTTTACC+TGG - chr6_3:23654653-23654672 Msa0945080:five_prime_UTR 45.0%
!! AAACATCGCCGTTTTACCTG+GGG - chr6_3:23654655-23654674 Msa0945080:five_prime_UTR 45.0%
AACGAAGTCCCCAGGTAAAA+CGG + chr6_3:23654666-23654685 None:intergenic 45.0%
GTCCTGAACAGCCGAAAGAA+AGG - chr6_3:23653475-23653494 Msa0945080:CDS 50.0%
GTCCTTTCTTTCGGCTGTTC+AGG + chr6_3:23653480-23653499 None:intergenic 50.0%
TTGATGTCACCGCCGGTATA+AGG + chr6_3:23653528-23653547 None:intergenic 50.0%
TGACTTCTTGATGTCACCGC+CGG + chr6_3:23653535-23653554 None:intergenic 50.0%
GCAAACGATCCAGTCCTTGA+AGG + chr6_3:23653633-23653652 None:intergenic 50.0%
TCAAGGACTGGATCGTTTGC+AGG - chr6_3:23653633-23653652 Msa0945080:five_prime_UTR 50.0%
CGTTCGGCTTATGTCACATC+AGG - chr6_3:23653699-23653718 Msa0945080:five_prime_UTR 50.0%
GAACCGGTTAAGAAGTCCTC+TGG - chr6_3:23653798-23653817 Msa0945080:intron 50.0%
GGACCAGAGGACTTCTTAAC+CGG + chr6_3:23653804-23653823 None:intergenic 50.0%
AAGTCCTCTGGTCCTCAATC+AGG - chr6_3:23653810-23653829 Msa0945080:intron 50.0%
!!! GAGGTATTGGACCCGAGTTT+TGG + chr6_3:23653848-23653867 None:intergenic 50.0%
CCAGTTGTTGGAAGAACTGG+AGG + chr6_3:23653867-23653886 None:intergenic 50.0%
CCTCCAGTTCTTCCAACAAC+TGG - chr6_3:23653864-23653883 Msa0945080:intron 50.0%
! TGAATTCATCTGGGGCTCCA+AGG - chr6_3:23653907-23653926 Msa0945080:intron 50.0%
ACACTGAGAGTAGTCACAGC+TGG + chr6_3:23653999-23654018 None:intergenic 50.0%
AGCTGTGACTACTCTCAGTG+TGG - chr6_3:23653998-23654017 Msa0945080:intron 50.0%
CTCTAATGGAACATTGCCGC+AGG + chr6_3:23654548-23654567 None:intergenic 50.0%
! CAGGTGCTGCAACAACTGGT+AGG + chr6_3:23652255-23652274 None:intergenic 55.0%
GTTGCCATGCCTTATACCGG+CGG - chr6_3:23653516-23653535 Msa0945080:CDS 55.0%
GGCGGTGACATCAAGAAGTC+AGG - chr6_3:23653534-23653553 Msa0945080:CDS 55.0%
GCAAAACGCAGCAGCTCGTT+CGG - chr6_3:23653683-23653702 Msa0945080:five_prime_UTR 55.0%
TCCTCTGGTCCTCAATCAGG+TGG - chr6_3:23653813-23653832 Msa0945080:intron 55.0%
ACCACCTGATTGAGGACCAG+AGG + chr6_3:23653817-23653836 None:intergenic 55.0%
TCTGGTCCTCAATCAGGTGG+TGG - chr6_3:23653816-23653835 Msa0945080:intron 55.0%
TGGTGGCACACGCCAAAACT+CGG - chr6_3:23653833-23653852 Msa0945080:intron 55.0%
TCTGGCCCGCTGAATTCATC+TGG - chr6_3:23653897-23653916 Msa0945080:intron 55.0%
CTGGCCCGCTGAATTCATCT+GGG - chr6_3:23653898-23653917 Msa0945080:intron 55.0%
TGGCCCGCTGAATTCATCTG+GGG - chr6_3:23653899-23653918 Msa0945080:intron 55.0%
GAGCCCCAGATGAATTCAGC+GGG + chr6_3:23653905-23653924 None:intergenic 55.0%
GGAGCCCCAGATGAATTCAG+CGG + chr6_3:23653906-23653925 None:intergenic 55.0%
! TTCATCTGGGGCTCCAAGGA+AGG - chr6_3:23653911-23653930 Msa0945080:intron 55.0%
! CGGTGGCGGCATTATTCACT+TGG - chr6_3:23654171-23654190 Msa0945080:intron 55.0%
GAGCTGAGGACAGCCAAGAA+TGG - chr6_3:23654251-23654270 Msa0945080:intron 55.0%
CTCAGTTGAACGAAGTCCCC+AGG + chr6_3:23654674-23654693 None:intergenic 55.0%
CTGGGGACTTCGTTCAACTG+AGG - chr6_3:23654672-23654691 Msa0945080:five_prime_UTR 55.0%
CAGGACGGCCCGATACATAC+AGG + chr6_3:23653461-23653480 None:intergenic 60.0%
GTCACCGCCGGTATAAGGCA+TGG + chr6_3:23653523-23653542 None:intergenic 60.0%
CGTGTGCCACCACCTGATTG+AGG + chr6_3:23653825-23653844 None:intergenic 60.0%
GGTGGCACACGCCAAAACTC+GGG - chr6_3:23653834-23653853 Msa0945080:intron 60.0%
GGGGCTCCAAGGAAGGTTTC+TGG - chr6_3:23653918-23653937 Msa0945080:intron 60.0%
GCTGTCCTCAGCTCTGCATC+AGG + chr6_3:23654245-23654264 None:intergenic 60.0%
GCAGGAGCTGCTTCGCACTC+TGG - chr6_3:23653651-23653670 Msa0945080:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 23652241 23654795 23652241 ID=Msa0945080;Name=Msa0945080
chr6_3 mRNA 23652241 23654795 23652241 ID=Msa0945080-mRNA-1;Parent=Msa0945080;Name=Msa0945080-mRNA-1;_AED=0.05;_eAED=0.05;_QI=390|1|0.66|1|0.5|0.33|3|0|377
chr6_3 exon 23652241 23652498 23652241 ID=Msa0945080-mRNA-1:exon:6631;Parent=Msa0945080-mRNA-1
chr6_3 exon 23652741 23653722 23652741 ID=Msa0945080-mRNA-1:exon:6630;Parent=Msa0945080-mRNA-1
chr6_3 exon 23654512 23654795 23654512 ID=Msa0945080-mRNA-1:exon:6629;Parent=Msa0945080-mRNA-1
chr6_3 five_prime_UTR 23654512 23654795 23654512 ID=Msa0945080-mRNA-1:five_prime_utr;Parent=Msa0945080-mRNA-1
chr6_3 five_prime_UTR 23653617 23653722 23653617 ID=Msa0945080-mRNA-1:five_prime_utr;Parent=Msa0945080-mRNA-1
chr6_3 CDS 23652741 23653616 23652741 ID=Msa0945080-mRNA-1:cds;Parent=Msa0945080-mRNA-1
chr6_3 CDS 23652241 23652498 23652241 ID=Msa0945080-mRNA-1:cds;Parent=Msa0945080-mRNA-1
Gene Sequence

>Msa0945080

ATGGGTTCGAGGTTCCCATCTCATCAGCTAAGCAATGGCCTGTATGTATCGGGCCGTCCTGAACAGCCGAAAGAAAGGACTCCAACAATGAGCTCAGTTGCCATGCCTTATACCGGCGGTGACATCAAGAAGTCAGGAGAATTAGGGAAAATGTTTGATATCCCTATGGATGGTTCCAAATCTAGAAAATCTGGTCCATTAAATAACGCTCCTTCAAGGACTGGATCGTTTGCAGGAGCTGCTTCGCACTCTGGTCCTATTATGCAAAACGCAGCAGCTCGTTCGGCTTATGTCACATCAGGTAATCTATCTGCTGCAGGCATGTCTACTTCAGCCTCAATGAAAAGGACAAACTCAGGTCCGTTGAATAAACACGGGGAACCGGTTAAGAAGTCCTCTGGTCCTCAATCAGGTGGTGGCACACGCCAAAACTCGGGTCCAATACCTCCAGTTCTTCCAACAACTGGTTTGATTACATCTGGCCCGCTGAATTCATCTGGGGCTCCAAGGAAGGTTTCTGGTCCATTAGAGTCCTTAGGGTCAATGAAATCAAATAGTGCTTCTGTAGTTCATAATCCAGCTGTGACTACTCTCAGTGTGGATGATGAATATTCCTTCAGGAAGAACTTCCCAAAGCCAATATTGTGGTCTGTGATTCTGATTTTCGTTATGGGATTCATTGCTGGAGGTTTTATTCTTGGAGCTGTCCGCAATGCCATACTCCTTATTGTTGTAGTAATTCTTTTTGCTGCGGTGGCGGCATTATTCACTTGGAATAGTTGTCGTGGAAGGAAAGCTATTGTTGGTTTCATTTCTCAATATCCTGATGCAGAGCTGAGGACAGCCAAGAATGGACAGTTTGTGAAGGTTTCTGGGGTGGTAACCTGCGGCAATGTTCCATTAGAGTCCTCCTTTCAAAAAGTTCCAAGATGTGTATATACATCCACTAGCTTGTATGAATATAGAGGATGGGATTCAAAAGCAGCTAACTCTAAACATCGCCGTTTTACCTGGGGACTTCGTTCAACTGAGGTAAGCAATAATCTGTTCAACATTTTCTTTTCAGATTGCCAAGTTTTATTTGAATTCGACAAAATATTGCGACTGCTTGAAATACTTTGGCCATGGATGTGA
Protein sequence

>Msa0945080

MGSRFPSHQLSNGLYVSGRPEQPKERTPTMSSVAMPYTGGDIKKSGELGKMFDIPMDGSKSRKSGPLNNAPSRTGSFAGAASHSGPIMQNAAARSAYVTSGNLSAAGMSTSASMKRTNSGPLNKHGEPVKKSSGPQSGGGTRQNSGPIPPVLPTTGLITSGPLNSSGAPRKVSGPLESLGSMKSNSASVVHNPAVTTLSVDDEYSFRKNFPKPILWSVILIFVMGFIAGGFILGAVRNAILLIVVVILFAAVAALFTWNSCRGRKAIVGFISQYPDAELRTAKNGQFVKVSGVVTCGNVPLESSFQKVPRCVYTSTSLYEYRGWDSKAANSKHRRFTWGLRSTEVSNNLFNIFFSDCQVLFEFDKILRLLEILWPWM*