AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0946500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946500 MtrunA17_Chr1g0209681 74.167 120 7 2 1 97 1 119 1.11e-48 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946500 AT1G57600.1 43.333 120 44 2 1 97 1 119 2.68e-25 98.6
Msa0946500 AT1G57600.2 43.333 120 44 2 1 97 1 119 2.68e-25 98.6
Msa0946500 AT1G57600.3 43.333 120 44 2 1 97 1 119 2.68e-25 98.6

Find 86 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGAATCTTTACACTTC+TGG 0.211962 6_3:-27446343 Msa0946500:CDS
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 6_3:+27446770 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 6_3:-27446887 Msa0946500:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 6_3:-27446360 Msa0946500:CDS
TTCTTCTTCCTTCGCGAATT+CGG 0.299784 6_3:+27447443 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 6_3:-27447511 Msa0946500:five_prime_UTR
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 6_3:+27446320 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 6_3:+27447460 None:intergenic
TCCAACGAGATTTGTTATTC+CGG 0.369458 6_3:+27447133 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 6_3:+27447517 None:intergenic
GAAAAGGGAAAAGGGAAAAG+TGG 0.376759 6_3:+27447716 None:intergenic
GAGAGATCAGACTTCTTAGT+TGG 0.380941 6_3:+27446963 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 6_3:-27446886 Msa0946500:five_prime_UTR
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 6_3:+27447518 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 6_3:+27447688 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 6_3:+27446842 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 6_3:+27447687 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 6_3:+27447651 None:intergenic
TCCGGCGAGGTGGCCGGTGG+TGG 0.424425 6_3:+27447151 None:intergenic
AGGGAAAGTGAAAAGGGAAA+AGG 0.433908 6_3:+27447707 None:intergenic
AGGGAAAAGGGAAAGTGAAA+AGG 0.448159 6_3:+27447700 None:intergenic
GGGAAAGTGAAAAGGGAAAA+GGG 0.449050 6_3:+27447708 None:intergenic
TGTTATTCCGGCGAGGTGGC+CGG 0.452930 6_3:+27447145 None:intergenic
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 6_3:+27447680 None:intergenic
GGGAAAAGGGAAAGTGAAAA+GGG 0.467752 6_3:+27447701 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 6_3:-27446249 Msa0946500:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 6_3:+27447326 None:intergenic
AGCAGCGATGGTTTCATAAG+AGG 0.471667 6_3:+27447057 None:intergenic
ATGCTCCATGCAAATAAGAT+AGG 0.474131 6_3:+27446244 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 6_3:+27446982 None:intergenic
GCCACCACCGGCCACCTCGC+CGG 0.476457 6_3:-27447152 Msa0946500:five_prime_UTR
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 6_3:-27446287 Msa0946500:CDS
AGAGATCAGACTTCTTAGTT+GGG 0.483449 6_3:+27446964 None:intergenic
TATTTGCATGGAGCATGGTA+TGG 0.486475 6_3:-27446237 Msa0946500:CDS
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 6_3:+27447670 None:intergenic
CTCGTTGGATAAATTTACAT+TGG 0.487759 6_3:-27447119 Msa0946500:five_prime_UTR
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 6_3:+27447605 None:intergenic
CGGCGCGGTGGTGCCACCAC+CGG 0.493775 6_3:-27447164 Msa0946500:five_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 6_3:+27446360 None:intergenic
CTGTACTCAAACCTGGAAAA+CGG 0.505341 6_3:-27446924 Msa0946500:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 6_3:+27446771 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 6_3:+27447646 None:intergenic
CGCTGCTTACAAACAACAGA+AGG 0.516592 6_3:-27447041 Msa0946500:five_prime_UTR
AAAAGGGAAAAGGGAAAAGT+GGG 0.520337 6_3:+27447717 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 6_3:+27447532 None:intergenic
CTGTTGTTTGTAAGCAGCGA+TGG 0.523025 6_3:+27447045 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 6_3:-27446329 Msa0946500:CDS
CTCTTGCGATTCCACGATTG+GGG 0.530968 6_3:-27446885 Msa0946500:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 6_3:-27446303 Msa0946500:CDS
CGGTGGTGGCACCACCGCGC+CGG 0.540387 6_3:+27447165 None:intergenic
AAAATAAGAGTTTGAAACTC+CGG 0.544922 6_3:-27447184 Msa0946500:five_prime_UTR
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 6_3:+27447671 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 6_3:+27447681 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 6_3:-27446328 Msa0946500:CDS
ATAAATTGTTCGATTGAGAA+CGG 0.563409 6_3:+27447642 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 6_3:+27447720 None:intergenic
TATCTTATTTGCATGGAGCA+TGG 0.574992 6_3:-27446242 Msa0946500:CDS
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 6_3:-27447361 Msa0946500:five_prime_UTR
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 6_3:+27446891 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 6_3:-27446931 Msa0946500:five_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 6_3:+27446874 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 6_3:+27447672 None:intergenic
GAAATGAGTTTAGAAAGTGA+AGG 0.599309 6_3:-27447235 Msa0946500:five_prime_UTR
GCCGGAATAACAAATCTCGT+TGG 0.609440 6_3:-27447134 Msa0946500:five_prime_UTR
CATCATCAGGCGCTCCCTCG+CGG 0.610543 6_3:-27446712 Msa0946500:intron
CTTCTTAGTTGGGAGATCGA+AGG 0.610786 6_3:+27446974 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 6_3:-27446918 Msa0946500:five_prime_UTR
CAACAACAAACACAAGCTGT+CGG 0.626988 6_3:-27447386 Msa0946500:five_prime_UTR
TATTCCGGCGAGGTGGCCGG+TGG 0.630081 6_3:+27447148 None:intergenic
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 6_3:-27446401 Msa0946500:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 6_3:-27447451 Msa0946500:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 6_3:-27447469 Msa0946500:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 6_3:+27447721 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 6_3:+27447596 None:intergenic
CGAGATTTGTTATTCCGGCG+AGG 0.652146 6_3:+27447138 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 6_3:+27447590 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 6_3:-27446814 Msa0946500:exon
AGAAAACAAAAGCCAGACAA+CGG 0.663004 6_3:+27446275 None:intergenic
AAAGAAGAAACATACCGCGA+GGG 0.665018 6_3:+27446698 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 6_3:+27446248 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 6_3:-27446788 Msa0946500:CDS
AGTTTGAAACTCCGGCGCGG+TGG 0.676359 6_3:-27447176 Msa0946500:five_prime_UTR
AGGATGTCTCAGATGCTCAG+TGG 0.679281 6_3:-27446404 Msa0946500:CDS
GATTTGTTATTCCGGCGAGG+TGG 0.679315 6_3:+27447141 None:intergenic
GCTGCTTACAAACAACAGAA+GGG 0.688406 6_3:-27447040 Msa0946500:five_prime_UTR
AAGAGTTTGAAACTCCGGCG+CGG 0.780575 6_3:-27447179 Msa0946500:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGTTTTTTATTTATCGT+CGG - chr6_3:27447228-27447247 Msa0946500:five_prime_UTR 15.0%
!!! ATTTGTTTTTTATTTATCGT+CGG - chr6_3:27447228-27447247 Msa0946500:five_prime_UTR 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr6_3:27446643-27446662 None:intergenic 20.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr6_3:27446643-27446662 None:intergenic 20.0%
! ATAAATTGTTCGATTGAGAA+CGG + chr6_3:27446203-27446222 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG - chr6_3:27446550-27446569 Msa0946500:intron 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr6_3:27446658-27446677 Msa0946500:intron 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr6_3:27446824-27446843 Msa0946500:five_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr6_3:27447117-27447136 Msa0946500:five_prime_UTR 25.0%
! CTGAAATACTTACAAAGAAA+TGG + chr6_3:27447348-27447367 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG + chr6_3:27447418-27447437 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr6_3:27447418-27447437 Msa0946500:five_prime_UTR 25.0%
! ATAAATTGTTCGATTGAGAA+CGG + chr6_3:27446203-27446222 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG - chr6_3:27446550-27446569 Msa0946500:intron 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr6_3:27446658-27446677 Msa0946500:intron 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr6_3:27446824-27446843 Msa0946500:five_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr6_3:27447117-27447136 Msa0946500:five_prime_UTR 25.0%
! CTGAAATACTTACAAAGAAA+TGG + chr6_3:27447348-27447367 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG + chr6_3:27447418-27447437 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr6_3:27447418-27447437 Msa0946500:five_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr6_3:27446495-27446514 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr6_3:27446607-27446626 Msa0946500:intron 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr6_3:27446723-27446742 Msa0946500:CDS 30.0%
CTACATAAACAGGTTATACA+TGG + chr6_3:27447177-27447196 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr6_3:27447187-27447206 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr6_3:27447256-27447275 Msa0946500:five_prime_UTR 30.0%
AATCATACATTGAGGTGATT+AGG + chr6_3:27447304-27447323 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr6_3:27447539-27447558 Msa0946500:five_prime_UTR 30.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr6_3:27446495-27446514 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr6_3:27446607-27446626 Msa0946500:intron 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr6_3:27446723-27446742 Msa0946500:CDS 30.0%
CTACATAAACAGGTTATACA+TGG + chr6_3:27447177-27447196 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr6_3:27447187-27447206 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr6_3:27447256-27447275 Msa0946500:five_prime_UTR 30.0%
AATCATACATTGAGGTGATT+AGG + chr6_3:27447304-27447323 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr6_3:27447539-27447558 Msa0946500:five_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG + chr6_3:27446128-27446147 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG + chr6_3:27446255-27446274 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG + chr6_3:27446313-27446332 None:intergenic 35.0%
!! TCCAACGAGATTTGTTATTC+CGG + chr6_3:27446712-27446731 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG - chr6_3:27446811-27446830 Msa0946500:exon 35.0%
AGAGATCAGACTTCTTAGTT+GGG + chr6_3:27446881-27446900 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr6_3:27447003-27447022 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr6_3:27447312-27447331 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr6_3:27447499-27447518 Msa0946500:five_prime_UTR 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr6_3:27447570-27447589 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr6_3:27447597-27447616 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr6_3:27447601-27447620 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr6_3:27447600-27447619 Msa0946500:five_prime_UTR 35.0%
AAAAGGGAAAAGGGAAAAGT+GGG + chr6_3:27446128-27446147 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG + chr6_3:27446255-27446274 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG + chr6_3:27446313-27446332 None:intergenic 35.0%
!! TCCAACGAGATTTGTTATTC+CGG + chr6_3:27446712-27446731 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG - chr6_3:27446811-27446830 Msa0946500:exon 35.0%
AGAGATCAGACTTCTTAGTT+GGG + chr6_3:27446881-27446900 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr6_3:27447003-27447022 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr6_3:27447312-27447331 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr6_3:27447499-27447518 Msa0946500:five_prime_UTR 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr6_3:27447570-27447589 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr6_3:27447597-27447616 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr6_3:27447601-27447620 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr6_3:27447600-27447619 Msa0946500:five_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG + chr6_3:27446129-27446148 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG + chr6_3:27446137-27446156 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG + chr6_3:27446138-27446157 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG + chr6_3:27446144-27446163 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG + chr6_3:27446145-27446164 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG + chr6_3:27446199-27446218 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG + chr6_3:27446240-27446259 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG + chr6_3:27446249-27446268 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG - chr6_3:27446373-27446392 Msa0946500:CDS 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG + chr6_3:27446385-27446404 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG + chr6_3:27446402-27446421 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr6_3:27446456-27446475 Msa0946500:intron 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr6_3:27446505-27446524 Msa0946500:intron 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr6_3:27446802-27446821 Msa0946500:CDS 40.0%
GAGAGATCAGACTTCTTAGT+TGG + chr6_3:27446882-27446901 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr6_3:27446911-27446930 Msa0946500:five_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr6_3:27446918-27446937 Msa0946500:five_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr6_3:27447028-27447047 Msa0946500:five_prime_UTR 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr6_3:27447074-27447093 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr6_3:27447075-27447094 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG + chr6_3:27447147-27447166 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr6_3:27447485-27447504 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr6_3:27447482-27447501 Msa0946500:five_prime_UTR 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr6_3:27447514-27447533 Msa0946500:five_prime_UTR 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr6_3:27447525-27447544 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr6_3:27447593-27447612 Msa0946500:five_prime_UTR 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr6_3:27447605-27447624 Msa0946500:five_prime_UTR 40.0%
GAAAAGGGAAAAGGGAAAAG+TGG + chr6_3:27446129-27446148 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG + chr6_3:27446137-27446156 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG + chr6_3:27446138-27446157 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG + chr6_3:27446144-27446163 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG + chr6_3:27446145-27446164 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG + chr6_3:27446199-27446218 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG + chr6_3:27446240-27446259 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG + chr6_3:27446249-27446268 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG - chr6_3:27446373-27446392 Msa0946500:CDS 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG + chr6_3:27446385-27446404 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG + chr6_3:27446402-27446421 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr6_3:27446456-27446475 Msa0946500:intron 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr6_3:27446505-27446524 Msa0946500:intron 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr6_3:27446802-27446821 Msa0946500:CDS 40.0%
GAGAGATCAGACTTCTTAGT+TGG + chr6_3:27446882-27446901 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr6_3:27446911-27446930 Msa0946500:five_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr6_3:27446918-27446937 Msa0946500:five_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr6_3:27447028-27447047 Msa0946500:five_prime_UTR 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr6_3:27447074-27447093 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr6_3:27447075-27447094 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG + chr6_3:27447147-27447166 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr6_3:27447485-27447504 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr6_3:27447482-27447501 Msa0946500:five_prime_UTR 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr6_3:27447514-27447533 Msa0946500:five_prime_UTR 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr6_3:27447525-27447544 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr6_3:27447593-27447612 Msa0946500:five_prime_UTR 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr6_3:27447605-27447624 Msa0946500:five_prime_UTR 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG + chr6_3:27446157-27446176 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG + chr6_3:27446158-27446177 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG + chr6_3:27446174-27446193 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG + chr6_3:27446175-27446194 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG + chr6_3:27446194-27446213 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG + chr6_3:27446327-27446346 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG + chr6_3:27446328-27446347 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG - chr6_3:27446331-27446350 Msa0946500:CDS 45.0%
AAAGGTAACCGAATTCGCGA+AGG - chr6_3:27446391-27446410 Msa0946500:CDS 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr6_3:27446519-27446538 None:intergenic 45.0%
GCCGGAATAACAAATCTCGT+TGG - chr6_3:27446708-27446727 Msa0946500:intron 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr6_3:27446788-27446807 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr6_3:27446800-27446819 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr6_3:27446801-27446820 Msa0946500:CDS 45.0%
CTTCTTAGTTGGGAGATCGA+AGG + chr6_3:27446871-27446890 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr6_3:27446956-27446975 Msa0946500:five_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr6_3:27447054-27447073 Msa0946500:five_prime_UTR 45.0%
GAAAGAAGAAACATACCGCG+AGG + chr6_3:27447148-27447167 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr6_3:27447267-27447286 Msa0946500:five_prime_UTR 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr6_3:27447289-27447308 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr6_3:27447513-27447532 Msa0946500:five_prime_UTR 45.0%
GAAGGGGAGAAAAGGGAAAA+GGG + chr6_3:27446157-27446176 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG + chr6_3:27446158-27446177 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG + chr6_3:27446174-27446193 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG + chr6_3:27446175-27446194 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG + chr6_3:27446194-27446213 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG + chr6_3:27446327-27446346 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG + chr6_3:27446328-27446347 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG - chr6_3:27446331-27446350 Msa0946500:CDS 45.0%
AAAGGTAACCGAATTCGCGA+AGG - chr6_3:27446391-27446410 Msa0946500:CDS 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr6_3:27446519-27446538 None:intergenic 45.0%
GCCGGAATAACAAATCTCGT+TGG - chr6_3:27446708-27446727 Msa0946500:intron 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr6_3:27446788-27446807 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr6_3:27446800-27446819 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr6_3:27446801-27446820 Msa0946500:CDS 45.0%
CTTCTTAGTTGGGAGATCGA+AGG + chr6_3:27446871-27446890 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr6_3:27446956-27446975 Msa0946500:five_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr6_3:27447054-27447073 Msa0946500:five_prime_UTR 45.0%
GAAAGAAGAAACATACCGCG+AGG + chr6_3:27447148-27447167 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr6_3:27447267-27447286 Msa0946500:five_prime_UTR 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr6_3:27447289-27447308 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr6_3:27447513-27447532 Msa0946500:five_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG + chr6_3:27446124-27446143 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG + chr6_3:27446125-27446144 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG + chr6_3:27446164-27446183 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG + chr6_3:27446165-27446184 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG + chr6_3:27446173-27446192 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr6_3:27446516-27446535 Msa0946500:intron 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr6_3:27446663-27446682 Msa0946500:intron 50.0%
!! GATTTGTTATTCCGGCGAGG+TGG + chr6_3:27446704-27446723 None:intergenic 50.0%
!! CGAGATTTGTTATTCCGGCG+AGG + chr6_3:27446707-27446726 None:intergenic 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr6_3:27446863-27446882 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr6_3:27446924-27446943 Msa0946500:five_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr6_3:27446954-27446973 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr6_3:27446955-27446974 Msa0946500:five_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr6_3:27446957-27446976 Msa0946500:five_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr6_3:27447268-27447287 Msa0946500:five_prime_UTR 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr6_3:27447288-27447307 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr6_3:27447438-27447457 Msa0946500:five_prime_UTR 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr6_3:27447441-27447460 Msa0946500:five_prime_UTR 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr6_3:27447452-27447471 Msa0946500:five_prime_UTR 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr6_3:27447555-27447574 Msa0946500:five_prime_UTR 50.0%
GGGAAAAGGGAAAAGTGGGA+GGG + chr6_3:27446124-27446143 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG + chr6_3:27446125-27446144 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG + chr6_3:27446164-27446183 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG + chr6_3:27446165-27446184 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG + chr6_3:27446173-27446192 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr6_3:27446516-27446535 Msa0946500:intron 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr6_3:27446663-27446682 Msa0946500:intron 50.0%
!! GATTTGTTATTCCGGCGAGG+TGG + chr6_3:27446704-27446723 None:intergenic 50.0%
!! CGAGATTTGTTATTCCGGCG+AGG + chr6_3:27446707-27446726 None:intergenic 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr6_3:27446863-27446882 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr6_3:27446924-27446943 Msa0946500:five_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr6_3:27446954-27446973 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr6_3:27446955-27446974 Msa0946500:five_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr6_3:27446957-27446976 Msa0946500:five_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr6_3:27447268-27447287 Msa0946500:five_prime_UTR 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr6_3:27447288-27447307 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr6_3:27447438-27447457 Msa0946500:five_prime_UTR 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr6_3:27447441-27447460 Msa0946500:five_prime_UTR 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr6_3:27447452-27447471 Msa0946500:five_prime_UTR 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr6_3:27447555-27447574 Msa0946500:five_prime_UTR 50.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr6_3:27446481-27446500 Msa0946500:intron 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr6_3:27446971-27446990 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr6_3:27447272-27447291 Msa0946500:five_prime_UTR 55.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr6_3:27446481-27446500 Msa0946500:intron 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr6_3:27446971-27446990 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr6_3:27447272-27447291 Msa0946500:five_prime_UTR 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr6_3:27446666-27446685 Msa0946500:intron 60.0%
TGTTATTCCGGCGAGGTGGC+CGG + chr6_3:27446700-27446719 None:intergenic 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr6_3:27446932-27446951 None:intergenic 60.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr6_3:27446666-27446685 Msa0946500:intron 60.0%
TGTTATTCCGGCGAGGTGGC+CGG + chr6_3:27446700-27446719 None:intergenic 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr6_3:27446932-27446951 None:intergenic 60.0%
CATCATCAGGCGCTCCCTCG+CGG - chr6_3:27447130-27447149 Msa0946500:five_prime_UTR 65.0%
CATCATCAGGCGCTCCCTCG+CGG - chr6_3:27447130-27447149 Msa0946500:five_prime_UTR 65.0%
TATTCCGGCGAGGTGGCCGG+TGG + chr6_3:27446697-27446716 None:intergenic 70.0%
TATTCCGGCGAGGTGGCCGG+TGG + chr6_3:27446697-27446716 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG + chr6_3:27446680-27446699 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG - chr6_3:27446678-27446697 Msa0946500:intron 80.0%
! GCCACCACCGGCCACCTCGC+CGG - chr6_3:27446690-27446709 Msa0946500:intron 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG + chr6_3:27446694-27446713 None:intergenic 80.0%
!! CGGTGGTGGCACCACCGCGC+CGG + chr6_3:27446680-27446699 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG - chr6_3:27446678-27446697 Msa0946500:intron 80.0%
! GCCACCACCGGCCACCTCGC+CGG - chr6_3:27446690-27446709 Msa0946500:intron 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG + chr6_3:27446694-27446713 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 27446109 27447755 27446109 ID=Msa0946500;Name=Msa0946500
chr6_3 mRNA 27446109 27447755 27446109 ID=Msa0946500-mRNA-1;Parent=Msa0946500;Name=Msa0946500-mRNA-1;_AED=0.04;_eAED=0.04;_QI=932|0|1|1|1|1|2|109|105
chr6_3 exon 27446109 27446424 27446109 ID=Msa0946500-mRNA-1:exon:7542;Parent=Msa0946500-mRNA-1
chr6_3 exon 27446713 27447755 27446713 ID=Msa0946500-mRNA-1:exon:7541;Parent=Msa0946500-mRNA-1
chr6_3 five_prime_UTR 27446824 27447755 27446824 ID=Msa0946500-mRNA-1:five_prime_utr;Parent=Msa0946500-mRNA-1
chr6_3 CDS 27446713 27446823 27446713 ID=Msa0946500-mRNA-1:cds;Parent=Msa0946500-mRNA-1
chr6_3 CDS 27446218 27446424 27446218 ID=Msa0946500-mRNA-1:cds;Parent=Msa0946500-mRNA-1
chr6_3 three_prime_UTR 27446109 27446217 27446109 ID=Msa0946500-mRNA-1:three_prime_utr;Parent=Msa0946500-mRNA-1
Gene Sequence

>Msa0946500

ATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGGTATGGAATTCATAGTTCATTTTGA
Protein sequence

>Msa0946500

MNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGAWYGIHSSF*