AlfalfaGEDB | Msa0948040

M. sativa cultivar zhongmu-4 / Msa0948040

KEGG: K03283
GO: -
PFAM: PF00012.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0948040	XP_013451705.1	90.141	71	7	0	2	72	3	73	2.89e-34	134

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0948040	sp\|Q9LHA8\|HS704_ARATH	66.667	72	23	1	1	72	1	71	1.20e-22	92.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0948040	A0A072U9G9	90.141	71	7	0	2	72	3	73	1.38e-34	134

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0754500	Msa0948040	0.800662	1.297569e-48	-8.615850e-47
Msa0830900	Msa0948040	0.815591	8.545145e-52	-8.615850e-47
Msa0948030	Msa0948040	0.853238	2.727715e-61	-8.615850e-47
Msa0948040	Msa0984470	0.921988	1.762194e-88	-8.615850e-47
Msa0948040	Msa0984480	0.807504	4.893325e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0948040	MtrunA17_Chr6g0462711	90.141	71	7	0	2	72	3	73	2.65e-38	134
Msa0948040	MtrunA17_Chr6g0462771	90.278	72	6	1	1	72	1	71	4.26e-37	130
Msa0948040	MtrunA17_Chr6g0462641	88.889	72	7	1	1	72	1	71	4.61e-36	127
Msa0948040	MtrunA17_Chr6g0462621	77.632	76	16	1	1	76	1	75	3.23e-35	114
Msa0948040	MtrunA17_Chr7g0223691	71.642	67	18	1	8	73	8	74	7.41e-29	98.2
Msa0948040	MtrunA17_Chr6g0462681	73.611	72	18	1	1	72	1	71	9.96e-29	106
Msa0948040	MtrunA17_Chr6g0462601	72.222	72	19	1	1	72	1	71	3.91e-28	105
Msa0948040	MtrunA17_Chr7g0223701	71.212	66	18	1	8	72	8	73	1.26e-24	95.1
Msa0948040	MtrunA17_Chr7g0223711	69.118	68	20	1	6	72	6	73	1.98e-24	94.7
Msa0948040	MtrunA17_Chr7g0248131	72.727	66	17	1	9	73	9	74	5.90e-24	93.2
Msa0948040	MtrunA17_Chr7g0223291	66.667	72	23	1	1	72	1	71	1.00e-23	92.4
Msa0948040	MtrunA17_Chr7g0223631	68.182	66	20	1	8	72	8	73	1.05e-23	92.4
Msa0948040	MtrunA17_Chr8g0350731	69.697	66	19	1	8	72	36	101	1.17e-23	92.4
Msa0948040	MtrunA17_Chr7g0223621	67.692	65	20	1	9	72	9	73	1.88e-23	91.7
Msa0948040	MtrunA17_Chr4g0031301	65.278	72	24	1	1	72	1	71	2.27e-23	91.7
Msa0948040	MtrunA17_Chr4g0031321	65.278	72	24	1	1	72	1	71	2.43e-23	91.7
Msa0948040	MtrunA17_Chr7g0223181	65.278	72	24	1	1	72	1	71	3.29e-23	91.3
Msa0948040	MtrunA17_Chr7g0245521	63.636	66	23	1	8	72	8	73	3.40e-23	89.0
Msa0948040	MtrunA17_Chr7g0223801	68.182	66	20	1	8	72	8	73	4.34e-23	90.9
Msa0948040	MtrunA17_Chr7g0262821	65.278	72	24	1	1	72	1	71	5.67e-23	90.5
Msa0948040	MtrunA17_Chr7g0223741	70.769	65	18	1	9	72	9	73	8.14e-23	90.1
Msa0948040	MtrunA17_Chr2g0299921	68.750	64	19	1	9	72	1413	1475	9.02e-23	89.7
Msa0948040	MtrunA17_Chr2g0299921	67.188	64	20	1	9	72	1029	1091	2.53e-22	88.6
Msa0948040	MtrunA17_Chr2g0299851	70.312	64	18	1	9	72	1038	1100	1.74e-22	89.0
Msa0948040	MtrunA17_Chr7g0223831	67.692	65	20	1	9	72	9	73	2.70e-22	88.6
Msa0948040	MtrunA17_Chr7g0215261	66.154	65	21	1	9	72	9	73	4.47e-22	87.8
Msa0948040	MtrunA17_Chr2g0287631	65.672	67	22	1	7	72	7	73	4.50e-22	87.8
Msa0948040	MtrunA17_Chr2g0293561	65.152	66	22	1	8	72	8	73	8.17e-22	86.7
Msa0948040	MtrunA17_Chr1g0188591	60.606	66	25	1	9	73	9	74	8.35e-22	80.9
Msa0948040	MtrunA17_Chr2g0299791	65.625	64	21	1	9	72	1042	1104	1.29e-21	86.7
Msa0948040	MtrunA17_Chr7g0223731	61.644	73	27	1	1	72	1	73	1.74e-21	86.3
Msa0948040	MtrunA17_Chr7g0223781	64.615	65	22	1	9	72	9	73	2.01e-21	85.9
Msa0948040	MtrunA17_Chr4g0073741	68.750	64	19	1	9	72	8	70	2.25e-21	85.9
Msa0948040	MtrunA17_Chr2g0296391	64.615	65	22	1	9	72	9	73	2.34e-21	85.9
Msa0948040	MtrunA17_Chr2g0293601	63.636	66	23	1	8	72	8	73	2.51e-21	85.5
Msa0948040	MtrunA17_Chr7g0223751	66.154	65	21	1	9	72	9	73	2.57e-21	85.9
Msa0948040	MtrunA17_Chr5g0427901	64.615	65	22	1	9	72	9	73	2.57e-21	85.9
Msa0948040	MtrunA17_Chr8g0388671	68.254	63	19	1	10	72	39	100	4.91e-21	85.1
Msa0948040	MtrunA17_Chr8g0388761	68.254	63	19	1	10	72	39	100	5.57e-21	84.7
Msa0948040	MtrunA17_Chr7g0233311	66.154	65	21	1	9	72	9	73	7.51e-21	84.3
Msa0948040	MtrunA17_Chr7g0233301	66.154	65	21	1	9	72	9	73	1.05e-20	84.0
Msa0948040	MtrunA17_Chr7g0228281	64.615	65	22	1	9	72	70	134	1.08e-20	84.0
Msa0948040	MtrunA17_Chr2g0299881	66.667	63	20	1	10	72	1039	1100	1.09e-20	84.0
Msa0948040	MtrunA17_Chr7g0225671	63.077	65	23	1	9	72	16	80	1.19e-20	84.0
Msa0948040	MtrunA17_Chr2g0293581	64.615	65	22	1	9	72	10	74	1.28e-20	83.6
Msa0948040	MtrunA17_Chr7g0223031	64.615	65	22	1	9	72	9	73	1.29e-20	83.6
Msa0948040	MtrunA17_Chr2g0284681	62.687	67	24	1	6	72	5	70	1.32e-20	83.6
Msa0948040	MtrunA17_Chr8g0390821	64.286	70	23	2	5	73	7	75	1.55e-20	83.6
Msa0948040	MtrunA17_Chr7g0217971	61.538	65	24	1	9	72	9	73	2.73e-20	82.8
Msa0948040	MtrunA17_Chr7g0223851	63.077	65	23	1	9	72	18	82	3.57e-20	82.4
Msa0948040	MtrunA17_Chr2g0298851	63.077	65	23	1	9	72	9	73	6.65e-20	81.6
Msa0948040	MtrunA17_Chr1g0151991	60.000	65	25	1	9	72	9	73	9.18e-20	80.1
Msa0948040	MtrunA17_Chr7g0223821	61.538	65	24	1	9	72	9	73	1.06e-19	81.3
Msa0948040	MtrunA17_Chr7g0221661	63.077	65	23	1	9	72	9	73	2.76e-19	80.1
Msa0948040	MtrunA17_Chr2g0299821	66.667	63	20	1	10	72	10	71	3.24e-19	79.7
Msa0948040	MtrunA17_Chr2g0331381	58.209	67	27	1	7	72	4	70	1.48e-18	77.8
Msa0948040	MtrunA17_Chr1g0166551	60.606	66	25	1	6	70	2	67	4.65e-18	76.3
Msa0948040	MtrunA17_Chr4g0040931	58.462	65	26	1	9	72	9	73	2.01e-17	74.7
Msa0948040	MtrunA17_Chr7g0223721	56.923	65	27	1	9	72	9	73	3.53e-17	73.9
Msa0948040	MtrunA17_Chr2g0298861	54.412	68	30	1	10	76	10	77	1.03e-16	72.8
Msa0948040	MtrunA17_Chr7g0248151	67.692	65	20	1	9	72	9	73	1.03e-15	69.7
Msa0948040	MtrunA17_Chr4g0049501	50.769	65	30	2	10	73	54	117	3.61e-12	59.7
Msa0948040	MtrunA17_Chr4g0012541	60.417	48	18	1	9	55	9	56	4.68e-12	59.3
Msa0948040	MtrunA17_Chr2g0293271	52.083	48	23	0	25	72	48	95	1.50e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0948040	AT5G02500.2	66.667	72	23	1	1	72	1	71	1.09e-23	92.8
Msa0948040	AT3G12580.1	66.667	72	23	1	1	72	1	71	1.22e-23	92.8
Msa0948040	AT5G02500.1	66.667	72	23	1	1	72	1	71	1.33e-23	92.8
Msa0948040	AT5G02490.1	65.278	72	24	1	1	72	1	71	3.14e-23	91.7
Msa0948040	AT1G56410.1	65.278	72	24	1	1	72	1	71	4.30e-23	91.3
Msa0948040	AT3G09440.1	65.278	72	24	1	1	72	1	71	6.63e-23	90.5
Msa0948040	AT3G09440.4	65.278	72	24	1	1	72	1	71	6.63e-23	90.5
Msa0948040	AT3G09440.2	65.278	72	24	1	1	72	1	71	6.63e-23	90.5
Msa0948040	AT3G09440.3	65.278	72	24	1	1	72	1	71	6.63e-23	90.5
Msa0948040	AT5G42020.3	68.254	63	19	1	10	72	43	104	6.58e-21	85.1
Msa0948040	AT5G28540.1	68.254	63	19	1	10	72	38	99	7.47e-21	84.7
Msa0948040	AT5G42020.1	68.254	63	19	1	10	72	38	99	9.62e-21	84.3
Msa0948040	AT5G42020.2	68.254	63	19	1	10	72	38	99	9.67e-21	84.3
Msa0948040	AT1G16030.1	68.750	64	19	1	9	72	8	70	1.09e-20	84.3
Msa0948040	AT1G09080.2	66.667	63	20	1	10	72	43	104	1.27e-19	81.3
Msa0948040	AT1G09080.1	66.667	63	20	1	10	72	53	114	1.30e-19	81.3
Msa0948040	AT4G37910.1	52.308	65	29	2	10	73	55	118	1.36e-12	61.2
Msa0948040	AT4G37910.2	52.308	65	29	2	10	73	55	118	1.36e-12	61.2
Msa0948040	AT5G09590.1	52.308	65	29	2	10	73	60	123	3.84e-11	57.4

Find 14 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAATAGTGAACGACTA+TGG	0.280824	6_3:-31704870	Msa0948040:CDS
TGGTGATGCTGCTTTCAATA+TGG	0.369722	6_3:-31704796	Msa0948040:CDS
ATAGGTAAGAAAGTAGCAAT+AGG	0.399869	6_3:-31704951	Msa0948040:CDS
AAGAAAGAAATGTCAGGAAT+AGG	0.412992	6_3:-31704969	None:intergenic
TACCGAAAATAGTGTTTGTT+GGG	0.413273	6_3:+31704763	None:intergenic
AAAGACTCTGAAAGAATGAT+TGG	0.419753	6_3:-31704816	Msa0948040:CDS
ACACACTGCAACACAAGAAT+AGG	0.454520	6_3:+31704911	None:intergenic
TTACCGAAAATAGTGTTTGT+TGG	0.469331	6_3:+31704762	None:intergenic
CACAAGAATAGGTTGTTCCT+AGG	0.516721	6_3:+31704922	None:intergenic
ATTGCAAAGAAAGAAATGTC+AGG	0.527573	6_3:-31704975	None:intergenic
ATATAATAGTGAACGACTAT+GGG	0.536102	6_3:-31704869	Msa0948040:CDS
TCTTTGAATGCAACACAAGA+AGG	0.585488	6_3:+31704834	None:intergenic
GTTGCAGTGTGTAAGAAGAA+TGG	0.586334	6_3:-31704900	Msa0948040:CDS
GTAGCAATAGGAATCGACCT+AGG	0.669365	6_3:-31704939	Msa0948040:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATAATAGTGAACGACTAT+GGG	-	chr6_3:31704843-31704862	Msa0948040:CDS	25.0%
	ATAGGTAAGAAAGTAGCAAT+AGG	-	chr6_3:31704761-31704780	Msa0948040:CDS	30.0%
	CATATAATAGTGAACGACTA+TGG	-	chr6_3:31704842-31704861	Msa0948040:CDS	30.0%
!	AAAGACTCTGAAAGAATGAT+TGG	-	chr6_3:31704896-31704915	Msa0948040:CDS	30.0%
!	AACCCAACAAACACTATTTT+CGG	-	chr6_3:31704947-31704966	Msa0948040:CDS	30.0%
	TACCGAAAATAGTGTTTGTT+GGG	+	chr6_3:31704952-31704971	None:intergenic	30.0%
	TTACCGAAAATAGTGTTTGT+TGG	+	chr6_3:31704953-31704972	None:intergenic	30.0%
	TCTTTGAATGCAACACAAGA+AGG	+	chr6_3:31704881-31704900	None:intergenic	35.0%
	CACAAGAATAGGTTGTTCCT+AGG	+	chr6_3:31704793-31704812	None:intergenic	40.0%
	ACACACTGCAACACAAGAAT+AGG	+	chr6_3:31704804-31704823	None:intergenic	40.0%
	GTTGCAGTGTGTAAGAAGAA+TGG	-	chr6_3:31704812-31704831	Msa0948040:CDS	40.0%
!!	TGGTGATGCTGCTTTCAATA+TGG	-	chr6_3:31704916-31704935	Msa0948040:CDS	40.0%
	GTAGCAATAGGAATCGACCT+AGG	-	chr6_3:31704773-31704792	Msa0948040:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	31704752	31704982	31704752	ID=Msa0948040;Name=Msa0948040
chr6_3	mRNA	31704752	31704982	31704752	ID=Msa0948040-mRNA-1;Parent=Msa0948040;Name=Msa0948040-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|76
chr6_3	exon	31704752	31704982	31704752	ID=Msa0948040-mRNA-1:exon:8323;Parent=Msa0948040-mRNA-1
chr6_3	CDS	31704752	31704982	31704752	ID=Msa0948040-mRNA-1:cds;Parent=Msa0948040-mRNA-1

Gene Sequence	>Msa0948040 ATGTCAGGAATAGGTAAGAAAGTAGCAATAGGAATCGACCTAGGAACAACCTATTCTTGTGTTGCAGTGTGTAAGAAGAATGGAGACGTACATATAATAGTGAACGACTATGGGAAAAGAACAACACCTTCTTGTGTTGCATTCAAAGACTCTGAAAGAATGATTGGTGATGCTGCTTTCAATATGGCTGCTTCGAACCCAACAAACACTATTTTCGGTAAAAGCTTCTAA
Protein sequence	>Msa0948040 MSGIGKKVAIGIDLGTTYSCVAVCKKNGDVHIIVNDYGKRTTPSCVAFKDSERMIGDAAFNMAASNPTNTIFGKSF*