AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0956530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0956530 MtrunA17_Chr1g0150121 87.634 186 5 2 1 168 1 186 1.11e-110 312
Msa0956530 MtrunA17_Chr3g0136861 33.484 221 89 8 1 167 1 217 1.10e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTATTGTTGTTGATTC+TGG 0.208831 6_3:-63936097 None:intergenic
TTCTCACCATTTGGGTTCTT+AGG 0.223638 6_3:+63940401 Msa0956530:three_prime_UTR
CTGTCTGCGTTTGAGTTTCA+TGG 0.247458 6_3:+63940365 Msa0956530:three_prime_UTR
TGAAATACGAACATTCAAAA+AGG 0.266282 6_3:-63936264 None:intergenic
TTCAGCGTCCTTGTTGCTTT+GGG 0.275461 6_3:+63940056 Msa0956530:three_prime_UTR
AGATGAATGCTTATTGCTTA+TGG 0.285315 6_3:+63940197 Msa0956530:three_prime_UTR
GTGATGCGTCAATAATTAAA+TGG 0.297952 6_3:+63940097 Msa0956530:three_prime_UTR
AATTTAATGTTCTCACCATT+TGG 0.301117 6_3:+63940392 Msa0956530:three_prime_UTR
GGAATTCAGGTAATTGAGTT+TGG 0.301722 6_3:-63936153 None:intergenic
TATTGCTTATGGCTTGCGAC+TGG 0.317936 6_3:+63940208 Msa0956530:three_prime_UTR
GAAGTCGTTCCAGTCTCTTT+TGG 0.333344 6_3:-63936722 None:intergenic
GTCAATAATTAAATGGCAAC+TGG 0.334356 6_3:+63940104 Msa0956530:three_prime_UTR
ATTTAATGTTCTCACCATTT+GGG 0.340283 6_3:+63940393 Msa0956530:three_prime_UTR
TCTGATAGAGCAAGGAATTC+AGG 0.352555 6_3:-63936166 None:intergenic
CACCATTTGGGTTCTTAGGT+TGG 0.364969 6_3:+63940405 Msa0956530:three_prime_UTR
AATATACCTTCACTCAAATC+TGG 0.367127 6_3:-63936744 None:intergenic
GTCACGTTCTCTGTTTCATT+TGG 0.368600 6_3:-63936067 None:intergenic
CTTTCTCTCTTCACTAGAAT+AGG 0.378897 6_3:-63936026 None:intergenic
AGAGAGAAAGAGAAAGAATA+TGG 0.380703 6_3:+63936039 Msa0956530:exon
GGGAGTGTCGCAAGATGATC+AGG 0.382472 6_3:-63936190 None:intergenic
GTTCAGCGTCCTTGTTGCTT+TGG 0.391508 6_3:+63940055 Msa0956530:three_prime_UTR
ATTGCTTATGGCTTGCGACT+GGG 0.406029 6_3:+63940209 Msa0956530:three_prime_UTR
CAAAAGTCATTATCTAACTT+TGG 0.413615 6_3:-63940303 None:intergenic
AGAGAGAATGAGCACGTTGA+AGG 0.421510 6_3:-63936216 None:intergenic
TCGAATTGTTCTTCGGAAGA+TGG 0.432800 6_3:-63938007 None:intergenic
GAGTACGTGGAGAAATTGCC+AGG 0.434070 6_3:+63938046 Msa0956530:CDS
TACAAACAGCAGCTCATAGC+TGG 0.435981 6_3:-63940172 None:intergenic
TCAACTACGTATATATTGTA+TGG 0.445908 6_3:+63940278 Msa0956530:three_prime_UTR
CAATTTCTCCACGTACTCCT+TGG 0.446252 6_3:-63938041 None:intergenic
AGCAGAACAGTGGCAAGATA+TGG 0.460848 6_3:+63937937 Msa0956530:intron
AATTTGATCGAATTGTTCTT+CGG 0.466862 6_3:-63938014 None:intergenic
AAGTGTGTGCCAAAAGAGAC+TGG 0.467228 6_3:+63936713 Msa0956530:CDS
CCAGGGGAGGATGTTGTTGG+TGG 0.491352 6_3:+63938064 Msa0956530:CDS
TTGCCAGGGGAGGATGTTGT+TGG 0.491487 6_3:+63938061 Msa0956530:CDS
TACAATATATACGTAGTTGA+AGG 0.499424 6_3:-63940275 None:intergenic
ATTCGATCAAATTGATTCCA+AGG 0.503194 6_3:+63938024 Msa0956530:CDS
TCAGCGTCCTTGTTGCTTTG+GGG 0.510939 6_3:+63940057 Msa0956530:three_prime_UTR
GAATGAGCACGTTGAAGGAG+TGG 0.521754 6_3:-63936211 None:intergenic
GGCCAACCTAAGAACCCAAA+TGG 0.527569 6_3:-63940407 None:intergenic
GTTGTTGGTGGTAGTAGTAG+TGG 0.528004 6_3:+63938076 Msa0956530:CDS
TGCAGTATATGTGAACTACA+TGG 0.528259 6_3:+63940014 Msa0956530:intron
GGTAGAAGTGAGTTTGGCGA+GGG 0.533143 6_3:-63936242 None:intergenic
TTGCTTATGGCTTGCGACTG+GGG 0.534371 6_3:+63940210 Msa0956530:three_prime_UTR
AGGAGCAGACTGGTCAAAAG+AGG 0.536792 6_3:+63937978 Msa0956530:CDS
TAGTAGTGGAAGCAGTATCA+AGG 0.540881 6_3:+63938090 Msa0956530:CDS
CATGAAACTCAAACGCAGAC+AGG 0.542569 6_3:-63940364 None:intergenic
AATGAGCACGTTGAAGGAGT+GGG 0.547922 6_3:-63936210 None:intergenic
GGGTAGAAGTGAGTTTGGCG+AGG 0.549628 6_3:-63936243 None:intergenic
TGGGGCGACTCATCAAAGCT+TGG 0.551107 6_3:+63940075 Msa0956530:three_prime_UTR
CGACTTCCAGATTTGAGTGA+AGG 0.560270 6_3:+63936738 Msa0956530:CDS
CCACCAACAACATCCTCCCC+TGG 0.586657 6_3:-63938064 None:intergenic
AGAACAGTGGCAAGATATGG+TGG 0.633543 6_3:+63937940 Msa0956530:intron
GGTTATTTCAAGCAGAACAG+TGG 0.634126 6_3:+63937927 Msa0956530:intron
GATCAGGGTCTGATAGAGCA+AGG 0.637548 6_3:-63936174 None:intergenic
CGTGGAGAAATTGCCAGGGG+AGG 0.640583 6_3:+63938051 Msa0956530:CDS
AATTGATTCCAAGGAGTACG+TGG 0.643895 6_3:+63938033 Msa0956530:CDS
AGTACGTGGAGAAATTGCCA+GGG 0.646997 6_3:+63938047 Msa0956530:CDS
TCAATAATTAAATGGCAACT+GGG 0.658227 6_3:+63940105 Msa0956530:three_prime_UTR
GGAGTGTCGCAAGATGATCA+GGG 0.666656 6_3:-63936189 None:intergenic
TGAGTCGCCCCAAAGCAACA+AGG 0.677678 6_3:-63940064 None:intergenic
TGATGACGATGATGATGATG+AGG 0.679554 6_3:+63938141 Msa0956530:CDS
GTACGTGGAGAAATTGCCAG+GGG 0.738965 6_3:+63938048 Msa0956530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAGTTACAATTTTTGA+AGG - chr6_3:63936310-63936329 None:intergenic 15.0%
!!! GTTTTTGTTGTTTTTTTTTT+TGG + chr6_3:63936364-63936383 Msa0956530:intron 15.0%
!!! CTATCTTAGTTTTTTTTTTA+GGG - chr6_3:63937663-63937682 None:intergenic 15.0%
!! TAATAAATATATCTCAAAGA+TGG - chr6_3:63938461-63938480 None:intergenic 15.0%
!!! TTTTGAAGGAAAACATTAAT+GGG - chr6_3:63936296-63936315 None:intergenic 20.0%
!!! TTTTTGAAGGAAAACATTAA+TGG - chr6_3:63936297-63936316 None:intergenic 20.0%
!! AAAACAACAAAAACGAAAAT+GGG - chr6_3:63936358-63936377 None:intergenic 20.0%
!! AAAAACAACAAAAACGAAAA+TGG - chr6_3:63936359-63936378 None:intergenic 20.0%
!! ATGCAACAAAATTAAAAACA+TGG - chr6_3:63936478-63936497 None:intergenic 20.0%
!! TGTTGCATACAAATAAAAAA+GGG + chr6_3:63937419-63937438 Msa0956530:intron 20.0%
!!! GCTATCTTAGTTTTTTTTTT+AGG - chr6_3:63937664-63937683 None:intergenic 20.0%
!! ATGTTAAGAATTTGATTGAA+TGG + chr6_3:63937812-63937831 Msa0956530:intron 20.0%
!! TGTTAAGAATTTGATTGAAT+GGG + chr6_3:63937813-63937832 Msa0956530:intron 20.0%
!! CATGTGTTTGAAAAAAAAAA+TGG + chr6_3:63937906-63937925 Msa0956530:intron 20.0%
!! CTACCAAAATACATAAAATA+TGG - chr6_3:63938238-63938257 None:intergenic 20.0%
!! TTATGATCTCTATCTAATTT+AGG + chr6_3:63938268-63938287 Msa0956530:intron 20.0%
!! TATGATCTCTATCTAATTTA+GGG + chr6_3:63938269-63938288 Msa0956530:intron 20.0%
!! ACATAATTAGTTAATTCGTT+TGG + chr6_3:63938770-63938789 Msa0956530:intron 20.0%
!!! AAAGGTAAAATTTGAGTTAT+TGG + chr6_3:63938969-63938988 Msa0956530:intron 20.0%
!! ATAAATTTCCATTGTAGTTA+TGG + chr6_3:63939050-63939069 Msa0956530:intron 20.0%
!!! AAGTATAGTTATTTCAGTTA+TGG + chr6_3:63939403-63939422 Msa0956530:intron 20.0%
!! GTAAACAAAGCAATTATATA+TGG - chr6_3:63939928-63939947 None:intergenic 20.0%
!!! TATATAATTGCTTTGTTTAC+TGG + chr6_3:63939928-63939947 Msa0956530:intron 20.0%
!!! CCTATAAATATTCTAGATTT+TGG - chr6_3:63939960-63939979 None:intergenic 20.0%
!!! CCAAAATCTAGAATATTTAT+AGG + chr6_3:63939957-63939976 Msa0956530:intron 20.0%
! GAAATACGAACATTCAAAAA+GGG - chr6_3:63936266-63936285 None:intergenic 25.0%
! TGAAATACGAACATTCAAAA+AGG - chr6_3:63936267-63936286 None:intergenic 25.0%
! TGAATGTAATCATATGTGTA+CGG - chr6_3:63936554-63936573 None:intergenic 25.0%
!!! TTGTTCAAGTTTTGACATTT+GGG + chr6_3:63936635-63936654 Msa0956530:intron 25.0%
!! CAAACTATTTTCATATAAGC+TGG + chr6_3:63936840-63936859 Msa0956530:intron 25.0%
!! TTATCATAAGTCCTTTTCAT+TGG + chr6_3:63937047-63937066 Msa0956530:intron 25.0%
! ACACTTAGAATGAAATATCA+TGG - chr6_3:63937098-63937117 None:intergenic 25.0%
! GTGTTGCATACAAATAAAAA+AGG + chr6_3:63937418-63937437 Msa0956530:intron 25.0%
! CATACAAATAAAAAAGGGAA+TGG + chr6_3:63937424-63937443 Msa0956530:intron 25.0%
!!! TTTTTTTTTTAGGGCAATAG+CGG - chr6_3:63937654-63937673 None:intergenic 25.0%
! AATTTGATCGAATTGTTCTT+CGG - chr6_3:63938017-63938036 None:intergenic 25.0%
! AATAACAGAAAACAGAGTTT+GGG - chr6_3:63938184-63938203 None:intergenic 25.0%
! CCAAAATACATAAAATATGG+AGG - chr6_3:63938235-63938254 None:intergenic 25.0%
!! CCTCCATATTTTATGTATTT+TGG + chr6_3:63938232-63938251 Msa0956530:intron 25.0%
!!! ATTGAGTTTTAGCAAAAGTT+CGG + chr6_3:63938491-63938510 Msa0956530:intron 25.0%
! TAATGAGAAAATGAAAGCTA+AGG - chr6_3:63938829-63938848 None:intergenic 25.0%
!!! TTTTGTTTAAAGCAGTTTAG+TGG - chr6_3:63938861-63938880 None:intergenic 25.0%
!! ATTGTTGGTTGAATTATTCA+AGG + chr6_3:63938944-63938963 Msa0956530:intron 25.0%
!! TTGTTGGTTGAATTATTCAA+GGG + chr6_3:63938945-63938964 Msa0956530:intron 25.0%
! AAATGTAATGATAAAGCTTG+TGG + chr6_3:63939238-63939257 Msa0956530:intron 25.0%
! AATGTAATGATAAAGCTTGT+GGG + chr6_3:63939239-63939258 Msa0956530:intron 25.0%
! CTACTCATTATTAATTGAGT+TGG - chr6_3:63939331-63939350 None:intergenic 25.0%
! CAACTCAATTAATAATGAGT+AGG + chr6_3:63939329-63939348 Msa0956530:intron 25.0%
!! ATAGTTATTTCAGTTATGGA+CGG + chr6_3:63939407-63939426 Msa0956530:intron 25.0%
! TCAATAATTAAATGGCAACT+GGG + chr6_3:63940105-63940124 Msa0956530:three_prime_UTR 25.0%
! TACAATATATACGTAGTTGA+AGG - chr6_3:63940278-63940297 None:intergenic 25.0%
! TCAACTACGTATATATTGTA+TGG + chr6_3:63940278-63940297 Msa0956530:three_prime_UTR 25.0%
! CAAAAGTCATTATCTAACTT+TGG - chr6_3:63940306-63940325 None:intergenic 25.0%
! AATTTAATGTTCTCACCATT+TGG + chr6_3:63940392-63940411 Msa0956530:three_prime_UTR 25.0%
! ATTTAATGTTCTCACCATTT+GGG + chr6_3:63940393-63940412 Msa0956530:three_prime_UTR 25.0%
AGAGAGAAAGAGAAAGAATA+TGG + chr6_3:63936039-63936058 Msa0956530:exon 30.0%
!! GATTCTATTGTTGTTGATTC+TGG - chr6_3:63936100-63936119 None:intergenic 30.0%
! ATTTAAAGGCATGGTCATTT+GGG - chr6_3:63936420-63936439 None:intergenic 30.0%
!! GTTGTTCAAGTTTTGACATT+TGG + chr6_3:63936634-63936653 Msa0956530:intron 30.0%
!! TGTTCAAGTTTTGACATTTG+GGG + chr6_3:63936636-63936655 Msa0956530:intron 30.0%
! GATTTTGTGTTCAACAGAAT+AGG + chr6_3:63936688-63936707 Msa0956530:intron 30.0%
AATATACCTTCACTCAAATC+TGG - chr6_3:63936747-63936766 None:intergenic 30.0%
GTTACTTATCCAAACAAACA+GGG - chr6_3:63936792-63936811 None:intergenic 30.0%
AGTTACTTATCCAAACAAAC+AGG - chr6_3:63936793-63936812 None:intergenic 30.0%
AAAAGGACTTATGATAAGCA+TGG - chr6_3:63937044-63937063 None:intergenic 30.0%
TAATAGCTCAACCAATGAAA+AGG - chr6_3:63937061-63937080 None:intergenic 30.0%
CATAAAAGAAAGACAGAACT+TGG - chr6_3:63937236-63937255 None:intergenic 30.0%
ATAATATCAGTAGTAAGCTG+TGG + chr6_3:63937287-63937306 Msa0956530:intron 30.0%
TGAGTCAACGGTATATAAAA+AGG + chr6_3:63937394-63937413 Msa0956530:intron 30.0%
ACAAATAAAAAAGGGAATGG+AGG + chr6_3:63937427-63937446 Msa0956530:intron 30.0%
!! AGATTTTGAGGGAAAAAACT+GGG - chr6_3:63937702-63937721 None:intergenic 30.0%
! TATAGCATAGCAGATTTTGA+GGG - chr6_3:63937713-63937732 None:intergenic 30.0%
AAGAATTTGATTGAATGGGT+TGG + chr6_3:63937817-63937836 Msa0956530:intron 30.0%
!! CTTTTTGTTTAACACTCTGT+TGG + chr6_3:63937851-63937870 Msa0956530:intron 30.0%
TCAAAATGTAAGACACGTAT+GGG - chr6_3:63937882-63937901 None:intergenic 30.0%
TTCAAAATGTAAGACACGTA+TGG - chr6_3:63937883-63937902 None:intergenic 30.0%
! ATTCGATCAAATTGATTCCA+AGG + chr6_3:63938024-63938043 Msa0956530:CDS 30.0%
AAACAGAGTTTGGGTAATAA+GGG - chr6_3:63938175-63938194 None:intergenic 30.0%
AAAACAGAGTTTGGGTAATA+AGG - chr6_3:63938176-63938195 None:intergenic 30.0%
CAATAACAGAAAACAGAGTT+TGG - chr6_3:63938185-63938204 None:intergenic 30.0%
GCAACGAAGTTAAAAATCAT+AGG + chr6_3:63938398-63938417 Msa0956530:intron 30.0%
GGTTATATGAGATTTGAATG+TGG + chr6_3:63938419-63938438 Msa0956530:intron 30.0%
TATATGAGATTTGAATGTGG+AGG + chr6_3:63938422-63938441 Msa0956530:intron 30.0%
TAATACGATTATACGACTGA+AGG + chr6_3:63938571-63938590 Msa0956530:intron 30.0%
AATACGATTATACGACTGAA+GGG + chr6_3:63938572-63938591 Msa0956530:intron 30.0%
!! TAGCGAGTTTTGATGTTTAT+CGG + chr6_3:63938637-63938656 Msa0956530:intron 30.0%
TTCATTTAGCAAGTGATCTT+AGG + chr6_3:63938803-63938822 Msa0956530:intron 30.0%
GTTGAATTATTCAAGGGAAA+AGG + chr6_3:63938951-63938970 Msa0956530:intron 30.0%
TACTAGTTAGATTTCCTGAA+TGG + chr6_3:63938997-63939016 Msa0956530:intron 30.0%
TCAAGATCCTTACACTAATA+AGG - chr6_3:63939031-63939050 None:intergenic 30.0%
GTGTATAAGTAGAACAAAAG+AGG + chr6_3:63939137-63939156 Msa0956530:intron 30.0%
! AACAAAAGAGGCATGAAATA+AGG + chr6_3:63939149-63939168 Msa0956530:intron 30.0%
GTGTTTGATTATTTGATGAG+TGG + chr6_3:63939202-63939221 Msa0956530:intron 30.0%
ATTATTTGATGAGTGGTTCT+GGG + chr6_3:63939209-63939228 Msa0956530:intron 30.0%
! TGAACTCTTAATTTAGCCTT+TGG + chr6_3:63939358-63939377 Msa0956530:intron 30.0%
! AATTTAGCCTTTGGTTATAG+AGG + chr6_3:63939367-63939386 Msa0956530:intron 30.0%
AATTGATCCTCTATAACCAA+AGG - chr6_3:63939377-63939396 None:intergenic 30.0%
AATCTAGAAGACAAAGGATA+TGG + chr6_3:63939714-63939733 Msa0956530:intron 30.0%
ATCTTTAGATCTAAACCTGA+AGG + chr6_3:63939737-63939756 Msa0956530:intron 30.0%
!!! CTTTTAAAACAAAAGCCTTC+AGG - chr6_3:63939755-63939774 None:intergenic 30.0%
!!! GCTTTTGTTTTAAAAGCCAA+AGG + chr6_3:63939759-63939778 Msa0956530:intron 30.0%
TTAGCCTGAAAATCATACTT+GGG + chr6_3:63939844-63939863 Msa0956530:intron 30.0%
GTGATGCGTCAATAATTAAA+TGG + chr6_3:63940097-63940116 Msa0956530:three_prime_UTR 30.0%
GTCAATAATTAAATGGCAAC+TGG + chr6_3:63940104-63940123 Msa0956530:three_prime_UTR 30.0%
AGATGAATGCTTATTGCTTA+TGG + chr6_3:63940197-63940216 Msa0956530:three_prime_UTR 30.0%
!! GGTTAACTATGCTTTTTAAC+TGG + chr6_3:63940231-63940250 Msa0956530:three_prime_UTR 30.0%
!!! GAATAGGTTTTGAGTCAACA+CGG - chr6_3:63936013-63936032 None:intergenic 35.0%
CTTTCTCTCTTCACTAGAAT+AGG - chr6_3:63936029-63936048 None:intergenic 35.0%
GGAATTCAGGTAATTGAGTT+TGG - chr6_3:63936156-63936175 None:intergenic 35.0%
AAAAAGGGTAGAAGTGAGTT+TGG - chr6_3:63936251-63936270 None:intergenic 35.0%
! GATTTAAAGGCATGGTCATT+TGG - chr6_3:63936421-63936440 None:intergenic 35.0%
GCTTACAAGCTCAGATTTAA+AGG - chr6_3:63936434-63936453 None:intergenic 35.0%
AATCTGAGCTTGTAAGCTAA+AGG + chr6_3:63936437-63936456 Msa0956530:intron 35.0%
ATCTGAGCTTGTAAGCTAAA+GGG + chr6_3:63936438-63936457 Msa0956530:intron 35.0%
! GCTAAAGGGTTCTTTTCTTT+TGG + chr6_3:63936452-63936471 Msa0956530:intron 35.0%
TTTCTTAGACCCTGTTTGTT+TGG + chr6_3:63936780-63936799 Msa0956530:intron 35.0%
AATAAGCGGAAACCAAGTTA+TGG + chr6_3:63936902-63936921 Msa0956530:intron 35.0%
CTTATGACACATCCATAACT+TGG - chr6_3:63936917-63936936 None:intergenic 35.0%
!!! TTTTAGAGTCCTACAGATGT+AGG - chr6_3:63937212-63937231 None:intergenic 35.0%
TAGTAAGCTGTGGTAAATAG+TGG + chr6_3:63937297-63937316 Msa0956530:intron 35.0%
!! CTATCTGCTATTTCACATAG+CGG - chr6_3:63937335-63937354 None:intergenic 35.0%
ATAAATGGTACACATAGTGC+AGG + chr6_3:63937546-63937565 Msa0956530:intron 35.0%
!! CAGATTTTGAGGGAAAAAAC+TGG - chr6_3:63937703-63937722 None:intergenic 35.0%
! GTATAGCATAGCAGATTTTG+AGG - chr6_3:63937714-63937733 None:intergenic 35.0%
CAAATAACAGTGTGCTATAG+CGG - chr6_3:63937736-63937755 None:intergenic 35.0%
! CACACTGTTATTTGACAACA+CGG + chr6_3:63937742-63937761 Msa0956530:intron 35.0%
! ACACTGTTATTTGACAACAC+GGG + chr6_3:63937743-63937762 Msa0956530:intron 35.0%
CAAAATGTAAGACACGTATG+GGG - chr6_3:63937881-63937900 None:intergenic 35.0%
TTTATTCTCACAAAACCCTC+AGG - chr6_3:63938354-63938373 None:intergenic 35.0%
!! AGTTTTAGCAAAAGTTCGGA+AGG + chr6_3:63938495-63938514 Msa0956530:intron 35.0%
!! TTCGGAAGGTATTTACCATA+GGG + chr6_3:63938509-63938528 Msa0956530:intron 35.0%
!! AGCGAGTTTTGATGTTTATC+GGG + chr6_3:63938638-63938657 Msa0956530:intron 35.0%
ATCAACAGTACACTAACAGA+AGG - chr6_3:63938692-63938711 None:intergenic 35.0%
AGGTTCACCTTATTAGTGTA+AGG + chr6_3:63939021-63939040 Msa0956530:intron 35.0%
TGTGCATTCCATAACTACAA+TGG - chr6_3:63939061-63939080 None:intergenic 35.0%
AATGCACACCTAATGTCTAT+GGG + chr6_3:63939073-63939092 Msa0956530:intron 35.0%
AGGCATGAAATAAGGCAATA+AGG + chr6_3:63939157-63939176 Msa0956530:intron 35.0%
AAATAAGGCAATAAGGTCAC+TGG + chr6_3:63939164-63939183 Msa0956530:intron 35.0%
GATTATTTGATGAGTGGTTC+TGG + chr6_3:63939208-63939227 Msa0956530:intron 35.0%
AATGTAACGATTGCCATTAC+GGG - chr6_3:63939516-63939535 None:intergenic 35.0%
GAATGTAACGATTGCCATTA+CGG - chr6_3:63939517-63939536 None:intergenic 35.0%
GGCAATCGTTACATTCTTAA+CGG + chr6_3:63939521-63939540 Msa0956530:intron 35.0%
TGTAAATCGTGAAAATGACG+TGG + chr6_3:63939599-63939618 Msa0956530:intron 35.0%
! GAACCATTAGGTCATCTTTT+GGG - chr6_3:63939672-63939691 None:intergenic 35.0%
! AGAACCATTAGGTCATCTTT+TGG - chr6_3:63939673-63939692 None:intergenic 35.0%
!! CAAGTATGATTTTCAGGCTA+AGG - chr6_3:63939845-63939864 None:intergenic 35.0%
CTTAGCCTGAAAATCATACT+TGG + chr6_3:63939843-63939862 Msa0956530:intron 35.0%
TAGCCTGAAAATCATACTTG+GGG + chr6_3:63939845-63939864 Msa0956530:intron 35.0%
TGCAGTATATGTGAACTACA+TGG + chr6_3:63940014-63940033 Msa0956530:intron 35.0%
!! GTTTTGAGTCAACACGGATT+TGG - chr6_3:63936007-63936026 None:intergenic 40.0%
GTCACGTTCTCTGTTTCATT+TGG - chr6_3:63936070-63936089 None:intergenic 40.0%
TCTGATAGAGCAAGGAATTC+AGG - chr6_3:63936169-63936188 None:intergenic 40.0%
ATAGTGAAGTTGAAGTGCAG+AGG + chr6_3:63936388-63936407 Msa0956530:intron 40.0%
TAGTGAAGTTGAAGTGCAGA+GGG + chr6_3:63936389-63936408 Msa0956530:intron 40.0%
CAAGCTCAGATTTAAAGGCA+TGG - chr6_3:63936429-63936448 None:intergenic 40.0%
GTCTGATCGAATGACATGTT+CGG + chr6_3:63936508-63936527 Msa0956530:intron 40.0%
!! AGATGTTCTGAAAGCTATCC+TGG + chr6_3:63936864-63936883 Msa0956530:intron 40.0%
GCAGAGCTTATGGAAATAAG+CGG + chr6_3:63936888-63936907 Msa0956530:intron 40.0%
! CACGGGTCTATTTTATGGAT+TGG - chr6_3:63937010-63937029 None:intergenic 40.0%
!! AAGAGCACGGGTCTATTTTA+TGG - chr6_3:63937015-63937034 None:intergenic 40.0%
AAGTCCTATCCTACATCTGT+AGG + chr6_3:63937200-63937219 Msa0956530:intron 40.0%
GCAGTAGCAGGAAAAAAATC+GGG - chr6_3:63937598-63937617 None:intergenic 40.0%
GGCAGTAGCAGGAAAAAAAT+CGG - chr6_3:63937599-63937618 None:intergenic 40.0%
! GGTTATTTCAAGCAGAACAG+TGG + chr6_3:63937927-63937946 Msa0956530:intron 40.0%
!! GGTGGAGCAAAAGTTTTATG+AGG + chr6_3:63937958-63937977 Msa0956530:CDS 40.0%
!! AAGTTTTATGAGGAGCAGAC+TGG + chr6_3:63937968-63937987 Msa0956530:CDS 40.0%
TCGAATTGTTCTTCGGAAGA+TGG - chr6_3:63938010-63938029 None:intergenic 40.0%
! AATTGATTCCAAGGAGTACG+TGG + chr6_3:63938033-63938052 Msa0956530:CDS 40.0%
! TAGTAGTGGAAGCAGTATCA+AGG + chr6_3:63938090-63938109 Msa0956530:CDS 40.0%
! TGATGACGATGATGATGATG+AGG + chr6_3:63938141-63938160 Msa0956530:CDS 40.0%
!! GTTCGGAAGGTATTTACCAT+AGG + chr6_3:63938508-63938527 Msa0956530:intron 40.0%
GACTCTTCACATATAACGCA+AGG - chr6_3:63938607-63938626 None:intergenic 40.0%
CAAAACTCGCTAAAAGAGTG+AGG - chr6_3:63938629-63938648 None:intergenic 40.0%
CAGTACACTAACAGAAGGTA+GGG - chr6_3:63938687-63938706 None:intergenic 40.0%
ACAGTACACTAACAGAAGGT+AGG - chr6_3:63938688-63938707 None:intergenic 40.0%
! GTTGATTGTACTGATGAGTG+CGG + chr6_3:63938706-63938725 Msa0956530:intron 40.0%
CCAACAATCATGAAAGAGAC+TGG - chr6_3:63938932-63938951 None:intergenic 40.0%
CCAGTCTCTTTCATGATTGT+TGG + chr6_3:63938929-63938948 Msa0956530:intron 40.0%
AGTTAGATTTCCTGAATGGC+AGG + chr6_3:63939001-63939020 Msa0956530:intron 40.0%
GAATGCACACCTAATGTCTA+TGG + chr6_3:63939072-63939091 Msa0956530:intron 40.0%
GGCCTTAAGCTTGAATAACT+GGG + chr6_3:63939094-63939113 Msa0956530:intron 40.0%
CACCCAGTTATTCAAGCTTA+AGG - chr6_3:63939099-63939118 None:intergenic 40.0%
!! AATAAGGTCACTGGTTCTAG+TGG + chr6_3:63939173-63939192 Msa0956530:intron 40.0%
GCTCATTAGTACAGTGATGA+TGG - chr6_3:63939290-63939309 None:intergenic 40.0%
ATGTAACGATTGCCATTACG+GGG - chr6_3:63939515-63939534 None:intergenic 40.0%
GTTACATTCTTAACGGCCTT+GGG + chr6_3:63939528-63939547 Msa0956530:intron 40.0%
!! ATGTGGAATAGAGGTGTTAG+GGG - chr6_3:63939567-63939586 None:intergenic 40.0%
!! CATGTGGAATAGAGGTGTTA+GGG - chr6_3:63939568-63939587 None:intergenic 40.0%
TCTATTCCACATGCTCTGTA+CGG + chr6_3:63939575-63939594 Msa0956530:intron 40.0%
GCTGGTTAATCATGAGATGA+AGG + chr6_3:63939634-63939653 Msa0956530:intron 40.0%
GATCCCAAAAGATGACCTAA+TGG + chr6_3:63939666-63939685 Msa0956530:intron 40.0%
TTCTGCAATGCAGAACCATT+AGG - chr6_3:63939684-63939703 None:intergenic 40.0%
GTTCTGCATTGCAGAAAGAA+GGG + chr6_3:63939688-63939707 Msa0956530:intron 40.0%
TTCTGCATTGCAGAAAGAAG+GGG + chr6_3:63939689-63939708 Msa0956530:intron 40.0%
GGGGAAAATCTAGAAGACAA+AGG + chr6_3:63939708-63939727 Msa0956530:intron 40.0%
CAGGATAGAGATATAATCCC+AGG - chr6_3:63939871-63939890 None:intergenic 40.0%
CTGGGATTATATCTCTATCC+TGG + chr6_3:63939869-63939888 Msa0956530:intron 40.0%
! TTCTCACCATTTGGGTTCTT+AGG + chr6_3:63940401-63940420 Msa0956530:three_prime_UTR 40.0%
AATGAGCACGTTGAAGGAGT+GGG - chr6_3:63936213-63936232 None:intergenic 45.0%
AGAGAGAATGAGCACGTTGA+AGG - chr6_3:63936219-63936238 None:intergenic 45.0%
TGTCATTCGATCAGACGTGT+CGG - chr6_3:63936504-63936523 None:intergenic 45.0%
! TTGTGTTGACGTGTCAGTGT+CGG + chr6_3:63936586-63936605 Msa0956530:intron 45.0%
AAGTGTGTGCCAAAAGAGAC+TGG + chr6_3:63936713-63936732 Msa0956530:CDS 45.0%
! GAAGTCGTTCCAGTCTCTTT+TGG - chr6_3:63936725-63936744 None:intergenic 45.0%
CGACTTCCAGATTTGAGTGA+AGG + chr6_3:63936738-63936757 Msa0956530:CDS 45.0%
ATTTCCATAAGCTCTGCACC+AGG - chr6_3:63936885-63936904 None:intergenic 45.0%
ACATTCCTCGTCTTTACTGC+TGG + chr6_3:63937136-63937155 Msa0956530:intron 45.0%
CAAAACCAGCAGTAAAGACG+AGG - chr6_3:63937144-63937163 None:intergenic 45.0%
GAGTCCTACAGATGTAGGAT+AGG - chr6_3:63937207-63937226 None:intergenic 45.0%
CAAATAGAGCAAGCCGCATA+GGG + chr6_3:63937364-63937383 Msa0956530:intron 45.0%
AAATAGAGCAAGCCGCATAG+GGG + chr6_3:63937365-63937384 Msa0956530:intron 45.0%
TAGGGGTTGGAATGAGTCAA+CGG + chr6_3:63937382-63937401 Msa0956530:intron 45.0%
!! GTTTCTGGTAGAGGATGTAG+TGG - chr6_3:63937620-63937639 None:intergenic 45.0%
TCTACCAGAAACTCGAACAG+CGG + chr6_3:63937628-63937647 Msa0956530:intron 45.0%
GGTTTCAGTTTACATTGCCG+AGG + chr6_3:63937764-63937783 Msa0956530:intron 45.0%
CAAAACGAAGACAACAGCCT+CGG - chr6_3:63937784-63937803 None:intergenic 45.0%
AGCAGAACAGTGGCAAGATA+TGG + chr6_3:63937937-63937956 Msa0956530:intron 45.0%
AGAACAGTGGCAAGATATGG+TGG + chr6_3:63937940-63937959 Msa0956530:intron 45.0%
CAATTTCTCCACGTACTCCT+TGG - chr6_3:63938044-63938063 None:intergenic 45.0%
AGTACGTGGAGAAATTGCCA+GGG + chr6_3:63938047-63938066 Msa0956530:CDS 45.0%
!! GTTGTTGGTGGTAGTAGTAG+TGG + chr6_3:63938076-63938095 Msa0956530:CDS 45.0%
! AGTTCTGATATGTGAGCCTG+AGG + chr6_3:63938335-63938354 Msa0956530:intron 45.0%
GTTCTGATATGTGAGCCTGA+GGG + chr6_3:63938336-63938355 Msa0956530:intron 45.0%
! CATTCTAGTTGCAAGCCCTA+TGG - chr6_3:63938527-63938546 None:intergenic 45.0%
ATAAGGTGAACCTGCCATTC+AGG - chr6_3:63939014-63939033 None:intergenic 45.0%
GCTTAAGGCCCATAGACATT+AGG - chr6_3:63939084-63939103 None:intergenic 45.0%
GGGCCTTAAGCTTGAATAAC+TGG + chr6_3:63939093-63939112 Msa0956530:intron 45.0%
!! ATTTGATGAGTGGTTCTGGG+AGG + chr6_3:63939212-63939231 Msa0956530:intron 45.0%
TTTCAGTTATGGACGGAGAG+TGG + chr6_3:63939414-63939433 Msa0956530:intron 45.0%
! TGGACACTTGATGACCGTTT+AGG + chr6_3:63939459-63939478 Msa0956530:intron 45.0%
GATGACCGTTTAGGTCGTAA+AGG + chr6_3:63939468-63939487 Msa0956530:intron 45.0%
!! GTTGGCCTTTACGACCTAAA+CGG - chr6_3:63939476-63939495 None:intergenic 45.0%
CGTTACATTCTTAACGGCCT+TGG + chr6_3:63939527-63939546 Msa0956530:intron 45.0%
!! GCATGTGGAATAGAGGTGTT+AGG - chr6_3:63939569-63939588 None:intergenic 45.0%
GTACAGAGCATGTGGAATAG+AGG - chr6_3:63939576-63939595 None:intergenic 45.0%
TTACATCCGTACAGAGCATG+TGG - chr6_3:63939584-63939603 None:intergenic 45.0%
CTCATGATTAACCAGCGTGT+AGG - chr6_3:63939630-63939649 None:intergenic 45.0%
GGTTCTGCATTGCAGAAAGA+AGG + chr6_3:63939687-63939706 Msa0956530:intron 45.0%
! AGGCCCCAAGTATGATTTTC+AGG - chr6_3:63939851-63939870 None:intergenic 45.0%
TGAAAATCATACTTGGGGCC+TGG + chr6_3:63939850-63939869 Msa0956530:intron 45.0%
GAAAATCATACTTGGGGCCT+GGG + chr6_3:63939851-63939870 Msa0956530:intron 45.0%
GACTACACAAGTTCTGTTCC+AGG - chr6_3:63939890-63939909 None:intergenic 45.0%
!! TTCAGCGTCCTTGTTGCTTT+GGG + chr6_3:63940056-63940075 Msa0956530:three_prime_UTR 45.0%
TACAAACAGCAGCTCATAGC+TGG - chr6_3:63940175-63940194 None:intergenic 45.0%
! TATTGCTTATGGCTTGCGAC+TGG + chr6_3:63940208-63940227 Msa0956530:three_prime_UTR 45.0%
! ATTGCTTATGGCTTGCGACT+GGG + chr6_3:63940209-63940228 Msa0956530:three_prime_UTR 45.0%
CATGAAACTCAAACGCAGAC+AGG - chr6_3:63940367-63940386 None:intergenic 45.0%
! CTGTCTGCGTTTGAGTTTCA+TGG + chr6_3:63940365-63940384 Msa0956530:three_prime_UTR 45.0%
! CACCATTTGGGTTCTTAGGT+TGG + chr6_3:63940405-63940424 Msa0956530:three_prime_UTR 45.0%
!!! TTTTAAAAATTTGTTATAAA+TGG + chr6_3:63937531-63937550 Msa0956530:intron 5.0%
GATCAGGGTCTGATAGAGCA+AGG - chr6_3:63936177-63936196 None:intergenic 50.0%
!! GGAGTGTCGCAAGATGATCA+GGG - chr6_3:63936192-63936211 None:intergenic 50.0%
GAATGAGCACGTTGAAGGAG+TGG - chr6_3:63936214-63936233 None:intergenic 50.0%
GGTAGAAGTGAGTTTGGCGA+GGG - chr6_3:63936245-63936264 None:intergenic 50.0%
!! ACATGTTCGGTGTCCGACAT+TGG + chr6_3:63936521-63936540 Msa0956530:intron 50.0%
CTATCCTGGTGCAGAGCTTA+TGG + chr6_3:63936878-63936897 Msa0956530:intron 50.0%
AGCATGGTCGAGAAAGAGCA+CGG - chr6_3:63937028-63937047 None:intergenic 50.0%
! ATGCGGCTTGCTCTATTTGC+AGG - chr6_3:63937363-63937382 None:intergenic 50.0%
GCAAATAGAGCAAGCCGCAT+AGG + chr6_3:63937363-63937382 Msa0956530:intron 50.0%
GACTCATTCCAACCCCTATG+CGG - chr6_3:63937380-63937399 None:intergenic 50.0%
!! GCTGTTCGAGTTTCTGGTAG+AGG - chr6_3:63937629-63937648 None:intergenic 50.0%
! AGGAGCAGACTGGTCAAAAG+AGG + chr6_3:63937978-63937997 Msa0956530:CDS 50.0%
GAGTACGTGGAGAAATTGCC+AGG + chr6_3:63938046-63938065 Msa0956530:CDS 50.0%
GTACGTGGAGAAATTGCCAG+GGG + chr6_3:63938048-63938067 Msa0956530:CDS 50.0%
! ACGACCTTTCAGGTCGAAGT+TGG + chr6_3:63939439-63939458 Msa0956530:intron 50.0%
GTGTCCAACTTCGACCTGAA+AGG - chr6_3:63939446-63939465 None:intergenic 50.0%
GGATAGGAGTTGCAGTAGGT+TGG - chr6_3:63939494-63939513 None:intergenic 50.0%
TGCAACTCCTATCCCCGTAA+TGG + chr6_3:63939500-63939519 Msa0956530:intron 50.0%
ACGATTGCCATTACGGGGAT+AGG - chr6_3:63939510-63939529 None:intergenic 50.0%
! GTTCAGCGTCCTTGTTGCTT+TGG + chr6_3:63940055-63940074 Msa0956530:three_prime_UTR 50.0%
!! TCAGCGTCCTTGTTGCTTTG+GGG + chr6_3:63940057-63940076 Msa0956530:three_prime_UTR 50.0%
! TTGCTTATGGCTTGCGACTG+GGG + chr6_3:63940210-63940229 Msa0956530:three_prime_UTR 50.0%
!! GGGAGTGTCGCAAGATGATC+AGG - chr6_3:63936193-63936212 None:intergenic 55.0%
GGGTAGAAGTGAGTTTGGCG+AGG - chr6_3:63936246-63936265 None:intergenic 55.0%
!! GTACGGTGTCGTGCCAATGT+CGG - chr6_3:63936537-63936556 None:intergenic 55.0%
! GCATGGTCGAGAAAGAGCAC+GGG - chr6_3:63937027-63937046 None:intergenic 55.0%
GAGGATGTAGTGGCAGTAGC+AGG - chr6_3:63937610-63937629 None:intergenic 55.0%
TTGCCAGGGGAGGATGTTGT+TGG + chr6_3:63938061-63938080 Msa0956530:CDS 55.0%
GAGAGTGGAGACGACCTTTC+AGG + chr6_3:63939429-63939448 Msa0956530:intron 55.0%
ACGGGGATAGGAGTTGCAGT+AGG - chr6_3:63939498-63939517 None:intergenic 55.0%
ACGTGGCTTATCCTACACGC+TGG + chr6_3:63939616-63939635 Msa0956530:intron 55.0%
ACACGAGACGCGTTCTCCTT+TGG - chr6_3:63939778-63939797 None:intergenic 55.0%
TGAGTCGCCCCAAAGCAACA+AGG - chr6_3:63940067-63940086 None:intergenic 55.0%
TGGGGCGACTCATCAAAGCT+TGG + chr6_3:63940075-63940094 Msa0956530:three_prime_UTR 55.0%
AGAGCAAGCCGCATAGGGGT+TGG + chr6_3:63937369-63937388 Msa0956530:intron 60.0%
CGTGGAGAAATTGCCAGGGG+AGG + chr6_3:63938051-63938070 Msa0956530:CDS 60.0%
CCACCAACAACATCCTCCCC+TGG - chr6_3:63938067-63938086 None:intergenic 60.0%
CCAGGGGAGGATGTTGTTGG+TGG + chr6_3:63938064-63938083 Msa0956530:CDS 60.0%
! GAGGTGTTAGGGGCCACAGA+AGG - chr6_3:63939557-63939576 None:intergenic 60.0%
! GCGGCCGCTGTTCGAGTTTC+TGG - chr6_3:63937635-63937654 None:intergenic 65.0%
CGGCCTTGGGACTCCTTCTG+TGG + chr6_3:63939541-63939560 Msa0956530:intron 65.0%
GGGCCACAGAAGGAGTCCCA+AGG - chr6_3:63939547-63939566 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 63935997 63940428 63935997 ID=Msa0956530;Name=Msa0956530
chr6_3 mRNA 63935997 63940428 63935997 ID=Msa0956530-mRNA-1;Parent=Msa0956530;Name=Msa0956530-mRNA-1;_AED=0.09;_eAED=0.09;_QI=61|0.75|0.6|1|0.5|0.6|5|401|168
chr6_3 exon 63935997 63936274 63935997 ID=Msa0956530-mRNA-1:exon:12209;Parent=Msa0956530-mRNA-1
chr6_3 exon 63936392 63936401 63936392 ID=Msa0956530-mRNA-1:exon:12210;Parent=Msa0956530-mRNA-1
chr6_3 exon 63936710 63936759 63936710 ID=Msa0956530-mRNA-1:exon:12211;Parent=Msa0956530-mRNA-1
chr6_3 exon 63937942 63938162 63937942 ID=Msa0956530-mRNA-1:exon:12212;Parent=Msa0956530-mRNA-1
chr6_3 exon 63940019 63940428 63940019 ID=Msa0956530-mRNA-1:exon:12213;Parent=Msa0956530-mRNA-1
chr6_3 five_prime_UTR 63935997 63936057 63935997 ID=Msa0956530-mRNA-1:five_prime_utr;Parent=Msa0956530-mRNA-1
chr6_3 CDS 63936058 63936274 63936058 ID=Msa0956530-mRNA-1:cds;Parent=Msa0956530-mRNA-1
chr6_3 CDS 63936392 63936401 63936392 ID=Msa0956530-mRNA-1:cds;Parent=Msa0956530-mRNA-1
chr6_3 CDS 63936710 63936759 63936710 ID=Msa0956530-mRNA-1:cds;Parent=Msa0956530-mRNA-1
chr6_3 CDS 63937942 63938162 63937942 ID=Msa0956530-mRNA-1:cds;Parent=Msa0956530-mRNA-1
chr6_3 CDS 63940019 63940027 63940019 ID=Msa0956530-mRNA-1:cds;Parent=Msa0956530-mRNA-1
chr6_3 three_prime_UTR 63940028 63940428 63940028 ID=Msa0956530-mRNA-1:three_prime_utr;Parent=Msa0956530-mRNA-1
Gene Sequence

>Msa0956530

ATGGTGAAACCAAATGAAACAGAGAACGTGACAGTACAACCAGAATCAACAACAATAGAATCACTCAAACGTGCTTTGTACAACGTTGAACAACTCCAAACTCAATTACCTGAATTCCTTGCTCTATCAGACCCTGATCATCTTGCGACACTCCCACTCCTTCAACGTGCTCATTCTCTCTTCTCCCTCGCCAAACTCACTTCTACCCTTTTTGAATTGAAGTTGAAGTTAAGTGTGTGCCAAAAGAGACTGGAACGACTTCCAGATTTGAGTGAAGAACAGTGGCAAGATATGGTGGAGCAAAAGTTTTATGAGGAGCAGACTGGTCAAAAGAGGAAGTATCCATCTTCCGAAGAACAATTCGATCAAATTGATTCCAAGGAGTACGTGGAGAAATTGCCAGGGGAGGATGTTGTTGGTGGTAGTAGTAGTGGAAGCAGTATCAAGGAAGCAATAATAATTGACTTATCAGATGATGATGACGATGATGATGATGAGTATATGTGA
Protein sequence

>Msa0956530

MVKPNETENVTVQPESTTIESLKRALYNVEQLQTQLPEFLALSDPDHLATLPLLQRAHSLFSLAKLTSTLFELKLKLSVCQKRLERLPDLSEEQWQDMVEQKFYEEQTGQKRKYPSSEEQFDQIDSKEYVEKLPGEDVVGGSSSGSSIKEAIIIDLSDDDDDDDDEYM*