AlfalfaGEDB | Msa0985630

M. sativa cultivar zhongmu-4 / Msa0985630

KEGG: K10878
GO: GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0008544 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030154 GO:0030855 GO:0031974 GO:0031981 GO:0032502 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045165 GO:0046483 GO:0048285 GO:0048856 GO:0048869 GO:0050896 GO:0051321 GO:0051716 GO:0060429 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0985630	MCH86545.1	67.532	77	25	0	1	77	178	254	3.61e-25	108

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0985630	sp\|Q9LZ03\|TOP6A_ARATH	48.649	74	33	1	5	73	248	321	2.36e-11	62.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0985630	A0A392MHH3	67.532	77	25	0	1	77	178	254	1.72e-25	108

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0397780	Msa0985630	0.834848	2.364106e-56	-8.615850e-47
Msa0399410	Msa0985630	0.802840	4.634510e-49	-8.615850e-47
Msa0985620	Msa0985630	0.881140	3.073209e-70	-8.615850e-47
Msa0985630	Msa0999120	0.813916	2.008784e-51	-8.615850e-47
Msa0351040	Msa0985630	0.822661	2.102639e-53	-8.615850e-47
Msa0910690	Msa0985630	0.876333	1.518365e-68	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0985630	MtrunA17_Chr1g0181791	50.000	74	32	1	5	73	238	311	8.57e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0985630	AT5G02820.1	48.649	74	33	1	5	73	248	321	2.40e-12	62.8

Find 30 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTAAAGTTTGGCTTGGTTT+TGG	0.277672	6_4:-32336516	None:intergenic
TTGAAGATGTTGGCTTTCTA+TGG	0.295610	6_4:+32336081	Msa0985630:CDS
ATTCAGTAGCAACATGAGTA+TGG	0.305636	6_4:-32335924	None:intergenic
AATGGTGTGCAACTAAAGTT+TGG	0.365662	6_4:-32336527	None:intergenic
TACTCATGTTGCTACTGAAT+TGG	0.380619	6_4:+32335927	Msa0985630:CDS
GTCCGCCTGCTTTCTTGATC+AGG	0.385261	6_4:+32336001	Msa0985630:CDS
TGAAACTGCAGCTTGAAGAT+TGG	0.391344	6_4:+32336361	Msa0985630:intron
TGATGCGCTCTTCATTCTCT+TGG	0.403238	6_4:+32335897	Msa0985630:CDS
CAAGATGCTAAAAGAGATGA+TGG	0.447185	6_4:+32336486	Msa0985630:CDS
GATTGGATTTGAAGCTAAGC+TGG	0.448987	6_4:+32336378	Msa0985630:CDS
GTATCCAGCAAGGCTAAAAC+AGG	0.473082	6_4:-32336045	None:intergenic
TTCAGTAGCAACATGAGTAT+GGG	0.510127	6_4:-32335923	None:intergenic
TCGATGTTCCTTGAAGATGT+TGG	0.513126	6_4:+32336071	Msa0985630:CDS
ATAGAAAGCCAACATCTTCA+AGG	0.514839	6_4:-32336079	None:intergenic
TGTATCGTTGTCTCCGCGGA+GGG	0.541313	6_4:+32335979	Msa0985630:CDS
TCCATCCTGATCAAGAAAGC+AGG	0.543181	6_4:-32336006	None:intergenic
GCCTGCTTTCTTGATCAGGA+TGG	0.543490	6_4:+32336005	Msa0985630:CDS
TGTTGGCTTTCTATGGCCGT+AGG	0.554576	6_4:+32336088	Msa0985630:CDS
TGTGCAACTAAAGTTTGGCT+TGG	0.585450	6_4:-32336522	None:intergenic
AAGCTAAGCTGGAAGAAGCA+AGG	0.595234	6_4:+32336389	Msa0985630:CDS
GGAAATGAAGCATCAAGCAA+AGG	0.600306	6_4:+32336456	Msa0985630:CDS
CATCTTCAAGGAACATCGAT+CGG	0.600343	6_4:-32336067	None:intergenic
GAAAGCAGGCGGACCCTCCG+CGG	0.610035	6_4:-32335992	None:intergenic
AAGCAATGAAGCTTTCAAAG+AGG	0.612694	6_4:+32336435	Msa0985630:CDS
ACATCGATCGGTATCCAGCA+AGG	0.624765	6_4:-32336055	None:intergenic
GTGTATCGTTGTCTCCGCGG+AGG	0.625580	6_4:+32335978	Msa0985630:CDS
TAGGTGTATCGTTGTCTCCG+CGG	0.634090	6_4:+32335975	Msa0985630:CDS
TCAGTAGCAACATGAGTATG+GGG	0.643252	6_4:-32335922	None:intergenic
ATCCTGATCAAGAAAGCAGG+CGG	0.660754	6_4:-32336003	None:intergenic
ACGATACACCTAAAGCGTTG+AGG	0.682803	6_4:-32335964	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATCATTTAATATATCTT+TGG	-	chr6_4:32336214-32336233	None:intergenic	10.0%
!!!	ACTTGAAATTTTTCAACCTA+CGG	-	chr6_4:32336107-32336126	None:intergenic	25.0%
!	GTAGGTTGAAAAATTTCAAG+TGG	+	chr6_4:32336106-32336125	Msa0985630:intron	30.0%
!	TTGAAAAATTTCAAGTGGCT+GGG	+	chr6_4:32336111-32336130	Msa0985630:intron	30.0%
	ATTTCAAAACTCGTCCAAAA+TGG	+	chr6_4:32336235-32336254	Msa0985630:intron	30.0%
	TTCAGTAGCAACATGAGTAT+GGG	-	chr6_4:32335926-32335945	None:intergenic	35.0%
	ATTCAGTAGCAACATGAGTA+TGG	-	chr6_4:32335927-32335946	None:intergenic	35.0%
	TACTCATGTTGCTACTGAAT+TGG	+	chr6_4:32335927-32335946	Msa0985630:CDS	35.0%
	ATAGAAAGCCAACATCTTCA+AGG	-	chr6_4:32336082-32336101	None:intergenic	35.0%
!!	TTGAAGATGTTGGCTTTCTA+TGG	+	chr6_4:32336081-32336100	Msa0985630:CDS	35.0%
!	GTTGAAAAATTTCAAGTGGC+TGG	+	chr6_4:32336110-32336129	Msa0985630:intron	35.0%
!	TGAAAAATTTCAAGTGGCTG+GGG	+	chr6_4:32336112-32336131	Msa0985630:intron	35.0%
	TATCATCTCCATGGATAGTA+AGG	+	chr6_4:32336176-32336195	Msa0985630:intron	35.0%
	CTCAATATCCTTACTATCCA+TGG	-	chr6_4:32336187-32336206	None:intergenic	35.0%
!	TGCAATTCGTCAATCCATTT+TGG	-	chr6_4:32336252-32336271	None:intergenic	35.0%
	AAGCAATGAAGCTTTCAAAG+AGG	+	chr6_4:32336435-32336454	Msa0985630:CDS	35.0%
	CAAGATGCTAAAAGAGATGA+TGG	+	chr6_4:32336486-32336505	Msa0985630:CDS	35.0%
	TCAGTAGCAACATGAGTATG+GGG	-	chr6_4:32335925-32335944	None:intergenic	40.0%
!!	GTTGTTTTCCTCAACGCTTT+AGG	+	chr6_4:32335956-32335975	Msa0985630:CDS	40.0%
	CATCTTCAAGGAACATCGAT+CGG	-	chr6_4:32336070-32336089	None:intergenic	40.0%
	TCGATGTTCCTTGAAGATGT+TGG	+	chr6_4:32336071-32336090	Msa0985630:CDS	40.0%
	TGCTCAGCTATAATATCCGT+TGG	-	chr6_4:32336144-32336163	None:intergenic	40.0%
	TCTTCAAGCTGCAGTTTCAT+TGG	-	chr6_4:32336360-32336379	None:intergenic	40.0%
	TGAAACTGCAGCTTGAAGAT+TGG	+	chr6_4:32336361-32336380	Msa0985630:intron	40.0%
!	GATTGGATTTGAAGCTAAGC+TGG	+	chr6_4:32336378-32336397	Msa0985630:CDS	40.0%
!	GGAAATGAAGCATCAAGCAA+AGG	+	chr6_4:32336456-32336475	Msa0985630:CDS	40.0%
	TGATGCGCTCTTCATTCTCT+TGG	+	chr6_4:32335897-32335916	Msa0985630:CDS	45.0%
!!	ACGATACACCTAAAGCGTTG+AGG	-	chr6_4:32335967-32335986	None:intergenic	45.0%
	ATCCTGATCAAGAAAGCAGG+CGG	-	chr6_4:32336006-32336025	None:intergenic	45.0%
	TCCATCCTGATCAAGAAAGC+AGG	-	chr6_4:32336009-32336028	None:intergenic	45.0%
!!!	ATTGCCTGTTTTAGCCTTGC+TGG	+	chr6_4:32336041-32336060	Msa0985630:CDS	45.0%
	GTATCCAGCAAGGCTAAAAC+AGG	-	chr6_4:32336048-32336067	None:intergenic	45.0%
!	ATTTCAAGTGGCTGGGGATT+AGG	+	chr6_4:32336118-32336137	Msa0985630:intron	45.0%
	TAGCTGTCGTATCATCTCCA+TGG	+	chr6_4:32336167-32336186	Msa0985630:intron	45.0%
	ATTGAGTCGCTGTGCTTAAC+CGG	+	chr6_4:32336303-32336322	Msa0985630:intron	45.0%
	TGTCAAGTACGATCTTACCC+CGG	-	chr6_4:32336325-32336344	None:intergenic	45.0%
	AAGCTAAGCTGGAAGAAGCA+AGG	+	chr6_4:32336389-32336408	Msa0985630:CDS	45.0%
	TAGGTGTATCGTTGTCTCCG+CGG	+	chr6_4:32335975-32335994	Msa0985630:CDS	50.0%
	GCCTGCTTTCTTGATCAGGA+TGG	+	chr6_4:32336005-32336024	Msa0985630:CDS	50.0%
	ACATCGATCGGTATCCAGCA+AGG	-	chr6_4:32336058-32336077	None:intergenic	50.0%
!!	TGTTGGCTTTCTATGGCCGT+AGG	+	chr6_4:32336088-32336107	Msa0985630:CDS	50.0%
	GGTTAAGCACAGCGACTCAA+TGG	-	chr6_4:32336304-32336323	None:intergenic	50.0%
	TTGAGTCGCTGTGCTTAACC+GGG	+	chr6_4:32336304-32336323	Msa0985630:intron	50.0%
	TGTATCGTTGTCTCCGCGGA+GGG	+	chr6_4:32335979-32335998	Msa0985630:CDS	55.0%
	GTCCGCCTGCTTTCTTGATC+AGG	+	chr6_4:32336001-32336020	Msa0985630:CDS	55.0%
	TGAGTCGCTGTGCTTAACCG+GGG	+	chr6_4:32336305-32336324	Msa0985630:intron	55.0%
	GTGTATCGTTGTCTCCGCGG+AGG	+	chr6_4:32335978-32335997	Msa0985630:CDS	60.0%
	GTGGCTGGGGATTAGGCCAA+CGG	+	chr6_4:32336125-32336144	Msa0985630:intron	60.0%
	GAAAGCAGGCGGACCCTCCG+CGG	-	chr6_4:32335995-32336014	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	32335886	32336537	32335886	ID=Msa0985630;Name=Msa0985630
chr6_4	mRNA	32335886	32336537	32335886	ID=Msa0985630-mRNA-1;Parent=Msa0985630;Name=Msa0985630-mRNA-1;_AED=0.78;_eAED=0.78;_QI=0\|0\|0\|0.5\|0\|0.5\|2\|0\|129
chr6_4	exon	32335886	32336109	32335886	ID=Msa0985630-mRNA-1:exon:8638;Parent=Msa0985630-mRNA-1
chr6_4	exon	32336372	32336537	32336372	ID=Msa0985630-mRNA-1:exon:8639;Parent=Msa0985630-mRNA-1
chr6_4	CDS	32335886	32336109	32335886	ID=Msa0985630-mRNA-1:cds;Parent=Msa0985630-mRNA-1
chr6_4	CDS	32336372	32336537	32336372	ID=Msa0985630-mRNA-1:cds;Parent=Msa0985630-mRNA-1

Gene Sequence

>Msa0985630

ATGGTCGCTGATGATGCGCTCTTCATTCTCTTGGTGCCCCATACTCATGTTGCTACTGAATTGGCTCGCTGTTGTTTTCCTCAACGCTTTAGGTGTATCGTTGTCTCCGCGGAGGGTCCGCCTGCTTTCTTGATCAGGATGGAGAAGAAATTGAAATTGCCTGTTTTAGCCTTGCTGGATACCGATCGATGTTCCTTGAAGATGTTGGCTTTCTATGGCCGTAGCTTGAAGATTGGATTTGAAGCTAAGCTGGAAGAAGCAAGGATTAGAGAAGAAAATCTGAACAGAAGCAATGAAGCTTTCAAAGAGGAAATGAAGCATCAAGCAAAGGCTACAAACAAGATGCTAAAAGAGATGATGGAGATGTTCCAAAACCAAGCCAAACTTTAG

Protein sequence

>Msa0985630

MVADDALFILLVPHTHVATELARCCFPQRFRCIVVSAEGPPAFLIRMEKKLKLPVLALLDTDRCSLKMLAFYGRSLKIGFEAKLEEARIREENLNRSNEAFKEEMKHQAKATNKMLKEMMEMFQNQAKL*