AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0988370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0988370 MtrunA17_Chr6g0468411 98.551 69 1 0 25 93 93 161 8.41e-44 140
Msa0988370 MtrunA17_Chr6g0468211 72.464 69 19 0 25 93 358 426 6.63e-23 91.7
Msa0988370 MtrunA17_Chr2g0303811 76.596 47 11 0 25 71 76 122 1.93e-20 80.1
Msa0988370 MtrunA17_Chr1g0155771 76.596 47 11 0 25 71 639 685 8.97e-19 79.7
Msa0988370 MtrunA17_Chr2g0277001 76.596 47 11 0 25 71 639 685 9.32e-19 79.7
Msa0988370 MtrunA17_Chr7g0275381 76.596 47 11 0 25 71 641 687 9.88e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0988370 AT3G56150.2 63.830 47 17 0 25 71 619 665 4.54e-15 69.7
Msa0988370 AT3G56150.1 63.830 47 17 0 25 71 619 665 4.54e-15 69.7
Msa0988370 AT3G22860.1 65.217 46 16 0 27 72 579 624 3.83e-14 67.0

Find 18 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAACATTGGCTGCAATAT+TGG 0.188251 6_4:+41466615 None:intergenic
GACATGATCCTTAACATTTC+AGG 0.258527 6_4:+41466514 None:intergenic
TGAACATTGGCTGCAATATT+GGG 0.333616 6_4:+41466616 None:intergenic
TTCCAGCTTCTTACTGAAAT+CGG 0.358742 6_4:+41467867 None:intergenic
CTAGGTATCTGCTATGCTAC+TGG 0.434675 6_4:-41466644 Msa0988370:intron
GTCACAAGGCTTCTTATCAA+TGG 0.457370 6_4:-41466485 Msa0988370:CDS
TGCCGATTTCAGTAAGAAGC+TGG 0.475568 6_4:-41467869 Msa0988370:CDS
ACGGTCCTCCTGAAATGTTA+AGG 0.511452 6_4:-41466522 Msa0988370:CDS
ATGCTTCGCATCATGAACAT+TGG 0.573990 6_4:+41466603 None:intergenic
CACTAATCTCGAGCAAGCGA+AGG 0.586081 6_4:+41466560 None:intergenic
TAATATTGTCAAAAGCCTTG+TGG 0.589992 6_4:+41466456 None:intergenic
CATCCTGATGATGAAGCTTG+AGG 0.604932 6_4:-41467920 None:intergenic
ATATTAAAGAGGTGGCACTT+AGG 0.610633 6_4:-41466439 Msa0988370:CDS
CACAAGAAATTCTAGACTCG+CGG 0.636437 6_4:+41467890 None:intergenic
ATCATGTCATGGTTGTCACA+AGG 0.644200 6_4:-41466499 Msa0988370:CDS
ATGATCCTTAACATTTCAGG+AGG 0.650670 6_4:+41466517 None:intergenic
AAATGTTAAGGATCATGTCA+TGG 0.662446 6_4:-41466510 Msa0988370:CDS
TATCAATGGAGACTTCCACA+AGG 0.685730 6_4:-41466471 Msa0988370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATTAATTACATTTGATA+TGG - chr6_4:41467242-41467261 Msa0988370:intron 15.0%
!!! AAATTCTTGATAATGTTAAT+TGG - chr6_4:41467433-41467452 Msa0988370:intron 15.0%
!! TGAATTAATTACATTTGATA+TGG - chr6_4:41467242-41467261 Msa0988370:intron 15.0%
!!! AAATTCTTGATAATGTTAAT+TGG - chr6_4:41467433-41467452 Msa0988370:intron 15.0%
!! AAACTCCTATATACAAAAAA+TGG + chr6_4:41466821-41466840 None:intergenic 20.0%
!! TTCAATTTAACAACATATGT+CGG - chr6_4:41467127-41467146 Msa0988370:intron 20.0%
!!! TGATATGGATTACAATATAT+TGG - chr6_4:41467257-41467276 Msa0988370:intron 20.0%
!!! ATATTTTACTTAACTTCGTT+TGG - chr6_4:41467373-41467392 Msa0988370:intron 20.0%
!! TTCTCTTTAGTACTATTATT+AGG - chr6_4:41467486-41467505 Msa0988370:intron 20.0%
!! TCTTAATTTACTTATCTTTC+AGG - chr6_4:41467598-41467617 Msa0988370:intron 20.0%
!! AAACTCCTATATACAAAAAA+TGG + chr6_4:41466821-41466840 None:intergenic 20.0%
!! TTCAATTTAACAACATATGT+CGG - chr6_4:41467127-41467146 Msa0988370:intron 20.0%
!!! TGATATGGATTACAATATAT+TGG - chr6_4:41467257-41467276 Msa0988370:intron 20.0%
!!! ATATTTTACTTAACTTCGTT+TGG - chr6_4:41467373-41467392 Msa0988370:intron 20.0%
!! TTCTCTTTAGTACTATTATT+AGG - chr6_4:41467486-41467505 Msa0988370:intron 20.0%
!! TCTTAATTTACTTATCTTTC+AGG - chr6_4:41467598-41467617 Msa0988370:intron 20.0%
! ATTTAAAGATCATTGACAGT+AGG - chr6_4:41466611-41466630 Msa0988370:CDS 25.0%
! CAGATTTCATTATCATCAAT+AGG + chr6_4:41466644-41466663 None:intergenic 25.0%
!! CTATTGATGATAATGAAATC+TGG - chr6_4:41466642-41466661 Msa0988370:CDS 25.0%
! TTGTCATTCAAATAATCAAC+AGG + chr6_4:41466749-41466768 None:intergenic 25.0%
! AAAGAAGAAAGATATGTTCT+CGG - chr6_4:41466787-41466806 Msa0988370:intron 25.0%
!!! ACTCACCATTTTTTGTATAT+AGG - chr6_4:41466813-41466832 Msa0988370:intron 25.0%
! TGCATTCTAAATGAACTTAA+TGG + chr6_4:41466855-41466874 None:intergenic 25.0%
!! ATGATTTTTGACATGGTTAA+TGG + chr6_4:41466992-41467011 None:intergenic 25.0%
!! ATGTATGATGATTTTTGACA+TGG + chr6_4:41466999-41467018 None:intergenic 25.0%
! GTCAAAAATCATCATACATT+AGG - chr6_4:41467000-41467019 Msa0988370:intron 25.0%
! ACAAAATATAACAGTGAACA+AGG + chr6_4:41467349-41467368 None:intergenic 25.0%
! TATCAAGAATTTAGTCTATC+AGG + chr6_4:41467425-41467444 None:intergenic 25.0%
! ATGCAAGTGATAAACTAAAT+AGG + chr6_4:41467522-41467541 None:intergenic 25.0%
!!! CACTTGCATTTTTTTTCAAT+CGG - chr6_4:41467533-41467552 Msa0988370:intron 25.0%
! ATTTACTTATCTTTCAGGAA+AGG - chr6_4:41467603-41467622 Msa0988370:intron 25.0%
! AGATGTATATGCATATGATA+TGG + chr6_4:41467650-41467669 None:intergenic 25.0%
! AGTGACAAAAAAACATTCAA+CGG - chr6_4:41467795-41467814 Msa0988370:intron 25.0%
!! TTTTGACAATATTAAAGAGG+TGG - chr6_4:41467889-41467908 Msa0988370:CDS 25.0%
! ATTTAAAGATCATTGACAGT+AGG - chr6_4:41466611-41466630 Msa0988370:CDS 25.0%
! CAGATTTCATTATCATCAAT+AGG + chr6_4:41466644-41466663 None:intergenic 25.0%
!! CTATTGATGATAATGAAATC+TGG - chr6_4:41466642-41466661 Msa0988370:CDS 25.0%
! TTGTCATTCAAATAATCAAC+AGG + chr6_4:41466749-41466768 None:intergenic 25.0%
! AAAGAAGAAAGATATGTTCT+CGG - chr6_4:41466787-41466806 Msa0988370:intron 25.0%
!!! ACTCACCATTTTTTGTATAT+AGG - chr6_4:41466813-41466832 Msa0988370:intron 25.0%
! TGCATTCTAAATGAACTTAA+TGG + chr6_4:41466855-41466874 None:intergenic 25.0%
!! ATGATTTTTGACATGGTTAA+TGG + chr6_4:41466992-41467011 None:intergenic 25.0%
!! ATGTATGATGATTTTTGACA+TGG + chr6_4:41466999-41467018 None:intergenic 25.0%
! GTCAAAAATCATCATACATT+AGG - chr6_4:41467000-41467019 Msa0988370:intron 25.0%
! ACAAAATATAACAGTGAACA+AGG + chr6_4:41467349-41467368 None:intergenic 25.0%
! TATCAAGAATTTAGTCTATC+AGG + chr6_4:41467425-41467444 None:intergenic 25.0%
! ATGCAAGTGATAAACTAAAT+AGG + chr6_4:41467522-41467541 None:intergenic 25.0%
!!! CACTTGCATTTTTTTTCAAT+CGG - chr6_4:41467533-41467552 Msa0988370:intron 25.0%
! ATTTACTTATCTTTCAGGAA+AGG - chr6_4:41467603-41467622 Msa0988370:intron 25.0%
! AGATGTATATGCATATGATA+TGG + chr6_4:41467650-41467669 None:intergenic 25.0%
! AGTGACAAAAAAACATTCAA+CGG - chr6_4:41467795-41467814 Msa0988370:intron 25.0%
!! TTTTGACAATATTAAAGAGG+TGG - chr6_4:41467889-41467908 Msa0988370:CDS 25.0%
ATGATAAAGCCTAAAAGAGA+TGG - chr6_4:41466676-41466695 Msa0988370:intron 30.0%
TGATAAAGCCTAAAAGAGAT+GGG - chr6_4:41466677-41466696 Msa0988370:intron 30.0%
!! TTAGTTTTCCCATCTCTTTT+AGG + chr6_4:41466688-41466707 None:intergenic 30.0%
GGTATACTATATGTCATCAA+AGG + chr6_4:41466900-41466919 None:intergenic 30.0%
TATAGTATACCTCAGCATTA+CGG - chr6_4:41466909-41466928 Msa0988370:intron 30.0%
AGAATAATTATTCACACTGG+AGG + chr6_4:41466955-41466974 None:intergenic 30.0%
CAGAGAATAATTATTCACAC+TGG + chr6_4:41466958-41466977 None:intergenic 30.0%
AAATCATCATACATTAGGTG+AGG - chr6_4:41467005-41467024 Msa0988370:intron 30.0%
ACTAACTCAGACATAGATAT+TGG - chr6_4:41467047-41467066 Msa0988370:intron 30.0%
! AAGCCTTATAGATGAAATGT+TGG - chr6_4:41467178-41467197 Msa0988370:intron 30.0%
AAATATAACAGTGAACAAGG+TGG + chr6_4:41467346-41467365 None:intergenic 30.0%
CAAGTGATAAACTAAATAGG+AGG + chr6_4:41467519-41467538 None:intergenic 30.0%
AAATGTTAAGGATCATGTCA+TGG - chr6_4:41467826-41467845 Msa0988370:intron 30.0%
TAATATTGTCAAAAGCCTTG+TGG + chr6_4:41467883-41467902 None:intergenic 30.0%
! GGCTTTTGACAATATTAAAG+AGG - chr6_4:41467886-41467905 Msa0988370:CDS 30.0%
ATGATAAAGCCTAAAAGAGA+TGG - chr6_4:41466676-41466695 Msa0988370:intron 30.0%
TGATAAAGCCTAAAAGAGAT+GGG - chr6_4:41466677-41466696 Msa0988370:intron 30.0%
!! TTAGTTTTCCCATCTCTTTT+AGG + chr6_4:41466688-41466707 None:intergenic 30.0%
GGTATACTATATGTCATCAA+AGG + chr6_4:41466900-41466919 None:intergenic 30.0%
TATAGTATACCTCAGCATTA+CGG - chr6_4:41466909-41466928 Msa0988370:intron 30.0%
AGAATAATTATTCACACTGG+AGG + chr6_4:41466955-41466974 None:intergenic 30.0%
CAGAGAATAATTATTCACAC+TGG + chr6_4:41466958-41466977 None:intergenic 30.0%
AAATCATCATACATTAGGTG+AGG - chr6_4:41467005-41467024 Msa0988370:intron 30.0%
ACTAACTCAGACATAGATAT+TGG - chr6_4:41467047-41467066 Msa0988370:intron 30.0%
! AAGCCTTATAGATGAAATGT+TGG - chr6_4:41467178-41467197 Msa0988370:intron 30.0%
AAATATAACAGTGAACAAGG+TGG + chr6_4:41467346-41467365 None:intergenic 30.0%
CAAGTGATAAACTAAATAGG+AGG + chr6_4:41467519-41467538 None:intergenic 30.0%
AAATGTTAAGGATCATGTCA+TGG - chr6_4:41467826-41467845 Msa0988370:intron 30.0%
TAATATTGTCAAAAGCCTTG+TGG + chr6_4:41467883-41467902 None:intergenic 30.0%
! GGCTTTTGACAATATTAAAG+AGG - chr6_4:41467886-41467905 Msa0988370:CDS 30.0%
TTCCAGCTTCTTACTGAAAT+CGG + chr6_4:41466472-41466491 None:intergenic 35.0%
GTTGCATTGAAGATCACATT+TGG + chr6_4:41466711-41466730 None:intergenic 35.0%
TAATTATTCACACTGGAGGA+AGG + chr6_4:41466951-41466970 None:intergenic 35.0%
! TACATGTTTGTGTCGGTATA+GGG + chr6_4:41467074-41467093 None:intergenic 35.0%
TTACGTCTACATGTTTGTGT+CGG + chr6_4:41467081-41467100 None:intergenic 35.0%
GCACCAACATTTCATCTATA+AGG + chr6_4:41467184-41467203 None:intergenic 35.0%
!! GAAATGTTGGTGCTACAAAT+TGG - chr6_4:41467191-41467210 Msa0988370:intron 35.0%
ACTTATCTTTCAGGAAAGGT+TGG - chr6_4:41467607-41467626 Msa0988370:intron 35.0%
!! TACCTAGATACACTGATTCT+AGG + chr6_4:41467679-41467698 None:intergenic 35.0%
! TGAACATTGGCTGCAATATT+GGG + chr6_4:41467723-41467742 None:intergenic 35.0%
! ATGAACATTGGCTGCAATAT+TGG + chr6_4:41467724-41467743 None:intergenic 35.0%
ATGATCCTTAACATTTCAGG+AGG + chr6_4:41467822-41467841 None:intergenic 35.0%
GACATGATCCTTAACATTTC+AGG + chr6_4:41467825-41467844 None:intergenic 35.0%
ATATTAAAGAGGTGGCACTT+AGG - chr6_4:41467897-41467916 Msa0988370:CDS 35.0%
TTCCAGCTTCTTACTGAAAT+CGG + chr6_4:41466472-41466491 None:intergenic 35.0%
GTTGCATTGAAGATCACATT+TGG + chr6_4:41466711-41466730 None:intergenic 35.0%
TAATTATTCACACTGGAGGA+AGG + chr6_4:41466951-41466970 None:intergenic 35.0%
! TACATGTTTGTGTCGGTATA+GGG + chr6_4:41467074-41467093 None:intergenic 35.0%
TTACGTCTACATGTTTGTGT+CGG + chr6_4:41467081-41467100 None:intergenic 35.0%
GCACCAACATTTCATCTATA+AGG + chr6_4:41467184-41467203 None:intergenic 35.0%
!! GAAATGTTGGTGCTACAAAT+TGG - chr6_4:41467191-41467210 Msa0988370:intron 35.0%
ACTTATCTTTCAGGAAAGGT+TGG - chr6_4:41467607-41467626 Msa0988370:intron 35.0%
!! TACCTAGATACACTGATTCT+AGG + chr6_4:41467679-41467698 None:intergenic 35.0%
! TGAACATTGGCTGCAATATT+GGG + chr6_4:41467723-41467742 None:intergenic 35.0%
! ATGAACATTGGCTGCAATAT+TGG + chr6_4:41467724-41467743 None:intergenic 35.0%
ATGATCCTTAACATTTCAGG+AGG + chr6_4:41467822-41467841 None:intergenic 35.0%
GACATGATCCTTAACATTTC+AGG + chr6_4:41467825-41467844 None:intergenic 35.0%
ATATTAAAGAGGTGGCACTT+AGG - chr6_4:41467897-41467916 Msa0988370:CDS 35.0%
CACAAGAAATTCTAGACTCG+CGG + chr6_4:41466449-41466468 None:intergenic 40.0%
! CTACATGTTTGTGTCGGTAT+AGG + chr6_4:41467075-41467094 None:intergenic 40.0%
AGTCTATCAGGTTAGTGTCT+AGG + chr6_4:41467413-41467432 None:intergenic 40.0%
CTCCTAGAATCAGTGTATCT+AGG - chr6_4:41467674-41467693 Msa0988370:intron 40.0%
ATGCTTCGCATCATGAACAT+TGG + chr6_4:41467736-41467755 None:intergenic 40.0%
ATCATGTCATGGTTGTCACA+AGG - chr6_4:41467837-41467856 Msa0988370:intron 40.0%
GTCACAAGGCTTCTTATCAA+TGG - chr6_4:41467851-41467870 Msa0988370:intron 40.0%
TATCAATGGAGACTTCCACA+AGG - chr6_4:41467865-41467884 Msa0988370:intron 40.0%
CACAAGAAATTCTAGACTCG+CGG + chr6_4:41466449-41466468 None:intergenic 40.0%
! CTACATGTTTGTGTCGGTAT+AGG + chr6_4:41467075-41467094 None:intergenic 40.0%
AGTCTATCAGGTTAGTGTCT+AGG + chr6_4:41467413-41467432 None:intergenic 40.0%
CTCCTAGAATCAGTGTATCT+AGG - chr6_4:41467674-41467693 Msa0988370:intron 40.0%
ATGCTTCGCATCATGAACAT+TGG + chr6_4:41467736-41467755 None:intergenic 40.0%
ATCATGTCATGGTTGTCACA+AGG - chr6_4:41467837-41467856 Msa0988370:intron 40.0%
GTCACAAGGCTTCTTATCAA+TGG - chr6_4:41467851-41467870 Msa0988370:intron 40.0%
TATCAATGGAGACTTCCACA+AGG - chr6_4:41467865-41467884 Msa0988370:intron 40.0%
!! TGCCGATTTCAGTAAGAAGC+TGG - chr6_4:41466467-41466486 Msa0988370:CDS 45.0%
TTTGTTGCACCGTAATGCTG+AGG + chr6_4:41466921-41466940 None:intergenic 45.0%
CTAGGTATCTGCTATGCTAC+TGG - chr6_4:41467692-41467711 Msa0988370:intron 45.0%
ACGGTCCTCCTGAAATGTTA+AGG - chr6_4:41467814-41467833 Msa0988370:intron 45.0%
!! TGCCGATTTCAGTAAGAAGC+TGG - chr6_4:41466467-41466486 Msa0988370:CDS 45.0%
TTTGTTGCACCGTAATGCTG+AGG + chr6_4:41466921-41466940 None:intergenic 45.0%
CTAGGTATCTGCTATGCTAC+TGG - chr6_4:41467692-41467711 Msa0988370:intron 45.0%
ACGGTCCTCCTGAAATGTTA+AGG - chr6_4:41467814-41467833 Msa0988370:intron 45.0%
!! CAAAATATATATAAATTTAT+GGG + chr6_4:41466516-41466535 None:intergenic 5.0%
!! ACAAAATATATATAAATTTA+TGG + chr6_4:41466517-41466536 None:intergenic 5.0%
!!! TTAATTTTCTTTAAAATTTT+AGG - chr6_4:41466578-41466597 Msa0988370:CDS 5.0%
!! CAAAATATATATAAATTTAT+GGG + chr6_4:41466516-41466535 None:intergenic 5.0%
!! ACAAAATATATATAAATTTA+TGG + chr6_4:41466517-41466536 None:intergenic 5.0%
!!! TTAATTTTCTTTAAAATTTT+AGG - chr6_4:41466578-41466597 Msa0988370:CDS 5.0%
!! AACATATGTCGGTGCTGTGC+AGG - chr6_4:41467138-41467157 Msa0988370:intron 50.0%
TGTGCAGGTATCAGTGTCAG+AGG - chr6_4:41467153-41467172 Msa0988370:intron 50.0%
CACTAATCTCGAGCAAGCGA+AGG + chr6_4:41467779-41467798 None:intergenic 50.0%
!! AACATATGTCGGTGCTGTGC+AGG - chr6_4:41467138-41467157 Msa0988370:intron 50.0%
TGTGCAGGTATCAGTGTCAG+AGG - chr6_4:41467153-41467172 Msa0988370:intron 50.0%
CACTAATCTCGAGCAAGCGA+AGG + chr6_4:41467779-41467798 None:intergenic 50.0%
! CAACAGGCTTTGTCGTCTGC+AGG + chr6_4:41466733-41466752 None:intergenic 55.0%
! CAACAGGCTTTGTCGTCTGC+AGG + chr6_4:41466733-41466752 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 41466426 41467932 41466426 ID=Msa0988370;Name=Msa0988370
chr6_4 mRNA 41466426 41467932 41466426 ID=Msa0988370-mRNA-1;Parent=Msa0988370;Name=Msa0988370-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|0.33|1|1|3|0|102
chr6_4 exon 41467867 41467932 41467867 ID=Msa0988370-mRNA-1:exon:9872;Parent=Msa0988370-mRNA-1
chr6_4 exon 41467753 41467758 41467753 ID=Msa0988370-mRNA-1:exon:9871;Parent=Msa0988370-mRNA-1
chr6_4 exon 41466426 41466662 41466426 ID=Msa0988370-mRNA-1:exon:9870;Parent=Msa0988370-mRNA-1
chr6_4 CDS 41467867 41467932 41467867 ID=Msa0988370-mRNA-1:cds;Parent=Msa0988370-mRNA-1
chr6_4 CDS 41467753 41467758 41467753 ID=Msa0988370-mRNA-1:cds;Parent=Msa0988370-mRNA-1
chr6_4 CDS 41466426 41466662 41466426 ID=Msa0988370-mRNA-1:cds;Parent=Msa0988370-mRNA-1
Gene Sequence

>Msa0988370

ATGAAGCTTGAGGAAGATGTCCGCGAGTCTAGAATTTCTTGTGCCGATTTCAGTAAGAAGCTGGAAGATGCAGTATCTGCTATGCTACTGGAAGTTCCCAATATTGCAGCCAATGTTCATGATGCGAAGCATAAGATCATCAGTAACAATTTCCTTCGCTTGCTCGAGATTAGTGACAAAAAAACATTCAACGGTCCTCCTGAAATGTTAAGGATCATGTCATGGTTGTCACAAGGCTTCTTATCAATGGAGACTTCCACAAGGCTTTTGACAATATTAAAGAGGTGGCACTTAGGACATACTTATTAA

Protein sequence

>Msa0988370

MKLEEDVRESRISCADFSKKLEDAVSAMLLEVPNIAANVHDAKHKIISNNFLRLLEISDKKTFNGPPEMLRIMSWLSQGFLSMETSTRLLTILKRWHLGHTY*