Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344520 | KEH29391.1 | 93.103 | 58 | 4 | 0 | 2 | 59 | 55 | 112 | 1.23e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344520 | A0A072UJ12 | 93.103 | 58 | 4 | 0 | 2 | 59 | 55 | 112 | 5.85e-21 | 87.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa1344520 | 0.833025 | 6.759774e-56 | -8.615850e-47 |
| Msa0016580 | Msa1344520 | 0.842049 | 3.278473e-58 | -8.615850e-47 |
| Msa0064970 | Msa1344520 | 0.825059 | 5.762134e-54 | -8.615850e-47 |
| Msa0102070 | Msa1344520 | 0.834717 | 2.550421e-56 | -8.615850e-47 |
| Msa0110260 | Msa1344520 | 0.829894 | 3.986083e-55 | -8.615850e-47 |
| Msa0110270 | Msa1344520 | 0.859959 | 2.882119e-63 | -8.615850e-47 |
| Msa1344520 | Msa1366810 | 0.801258 | 9.802978e-49 | -8.615850e-47 |
| Msa1344520 | Msa1386330 | 0.800137 | 1.659197e-48 | -8.615850e-47 |
| Msa1344520 | Msa1397580 | 0.825751 | 3.950935e-54 | -8.615850e-47 |
| Msa1344520 | Msa1402790 | 0.836004 | 1.206204e-56 | -8.615850e-47 |
| Msa1344520 | Msa1404000 | 0.821320 | 4.298241e-53 | -8.615850e-47 |
| Msa1344520 | Msa1422180 | 0.879603 | 1.089132e-69 | -8.615850e-47 |
| Msa1344520 | Msa1433120 | 0.831352 | 1.752584e-55 | -8.615850e-47 |
| Msa0604350 | Msa1344520 | 0.817325 | 3.496639e-52 | -8.615850e-47 |
| Msa0636170 | Msa1344520 | 0.816042 | 6.782040e-52 | -8.615850e-47 |
| Msa0639610 | Msa1344520 | 0.812850 | 3.444349e-51 | -8.615850e-47 |
| Msa0694370 | Msa1344520 | 0.841270 | 5.261499e-58 | -8.615850e-47 |
| Msa0726140 | Msa1344520 | 0.823432 | 1.389355e-53 | -8.615850e-47 |
| Msa0729800 | Msa1344520 | 0.836808 | 7.533404e-57 | -8.615850e-47 |
| Msa0733290 | Msa1344520 | 0.809308 | 2.017520e-50 | -8.615850e-47 |
| Msa0743810 | Msa1344520 | 0.846378 | 2.255125e-59 | -8.615850e-47 |
| Msa0745060 | Msa1344520 | 0.814131 | 1.801037e-51 | -8.615850e-47 |
| Msa0748970 | Msa1344520 | 0.809198 | 2.129917e-50 | -8.615850e-47 |
| Msa0767720 | Msa1344520 | 0.810187 | 1.306035e-50 | -8.615850e-47 |
| Msa0768290 | Msa1344520 | 0.800472 | 1.418139e-48 | -8.615850e-47 |
| Msa0768370 | Msa1344520 | 0.830171 | 3.412566e-55 | -8.615850e-47 |
| Msa0783160 | Msa1344520 | 0.828540 | 8.494004e-55 | -8.615850e-47 |
| Msa0784290 | Msa1344520 | 0.814669 | 1.369556e-51 | -8.615850e-47 |
| Msa0784300 | Msa1344520 | 0.816646 | 4.966936e-52 | -8.615850e-47 |
| Msa0808010 | Msa1344520 | 0.841699 | 4.056498e-58 | -8.615850e-47 |
| Msa0808020 | Msa1344520 | 0.835164 | 1.968173e-56 | -8.615850e-47 |
| Msa0824590 | Msa1344520 | 0.809906 | 1.501141e-50 | -8.615850e-47 |
| Msa0824600 | Msa1344520 | 0.812348 | 4.434890e-51 | -8.615850e-47 |
| Msa0825860 | Msa1344520 | 0.822227 | 2.651850e-53 | -8.615850e-47 |
| Msa0123810 | Msa1344520 | 0.812757 | 3.609809e-51 | -8.615850e-47 |
| Msa0200000 | Msa1344520 | 0.817771 | 2.773811e-52 | -8.615850e-47 |
| Msa0227480 | Msa1344520 | 0.815558 | 8.692203e-52 | -8.615850e-47 |
| Msa0232850 | Msa1344520 | 0.827785 | 1.291085e-54 | -8.615850e-47 |
| Msa1246880 | Msa1344520 | 0.822374 | 2.451040e-53 | -8.615850e-47 |
| Msa1248300 | Msa1344520 | 0.829058 | 6.365795e-55 | -8.615850e-47 |
| Msa1248310 | Msa1344520 | 0.842593 | 2.352182e-58 | -8.615850e-47 |
| Msa1248370 | Msa1344520 | 0.828439 | 8.985849e-55 | -8.615850e-47 |
| Msa1269670 | Msa1344520 | 0.811036 | 8.557196e-51 | -8.615850e-47 |
| Msa1270230 | Msa1344520 | 0.821086 | 4.867167e-53 | -8.615850e-47 |
| Msa1270240 | Msa1344520 | 0.801199 | 1.007795e-48 | -8.615850e-47 |
| Msa1283330 | Msa1344520 | 0.833250 | 5.939089e-56 | -8.615850e-47 |
| Msa1285540 | Msa1344520 | 0.803333 | 3.665682e-49 | -8.615850e-47 |
| Msa1285930 | Msa1344520 | 0.845510 | 3.883852e-59 | -8.615850e-47 |
| Msa1287800 | Msa1344520 | 0.849211 | 3.739131e-60 | -8.615850e-47 |
| Msa1287810 | Msa1344520 | 0.854587 | 1.114699e-61 | -8.615850e-47 |
| Msa1289430 | Msa1344520 | 0.807256 | 5.523464e-50 | -8.615850e-47 |
| Msa1320300 | Msa1344520 | 0.817875 | 2.628747e-52 | -8.615850e-47 |
| Msa1340590 | Msa1344520 | 0.819679 | 1.023513e-52 | -8.615850e-47 |
| Msa0365500 | Msa1344520 | 0.807308 | 5.387100e-50 | -8.615850e-47 |
| Msa0384800 | Msa1344520 | 0.814981 | 1.168225e-51 | -8.615850e-47 |
| Msa0384890 | Msa1344520 | 0.820243 | 7.601106e-53 | -8.615850e-47 |
| Msa0414410 | Msa1344520 | 0.840516 | 8.296285e-58 | -8.615850e-47 |
| Msa0414420 | Msa1344520 | 0.848161 | 7.311574e-60 | -8.615850e-47 |
| Msa0444130 | Msa1344520 | 0.821923 | 3.117930e-53 | -8.615850e-47 |
| Msa0450000 | Msa1344520 | 0.813697 | 2.244477e-51 | -8.615850e-47 |
| Msa0450570 | Msa1344520 | 0.804592 | 2.006328e-49 | -8.615850e-47 |
| Msa0452900 | Msa1344520 | 0.846928 | 1.596124e-59 | -8.615850e-47 |
| Msa0459160 | Msa1344520 | 0.812862 | 3.424271e-51 | -8.615850e-47 |
| Msa0479340 | Msa1344520 | 0.834210 | 3.418541e-56 | -8.615850e-47 |
| Msa0979850 | Msa1344520 | 0.800583 | 1.346204e-48 | -8.615850e-47 |
| Msa0981370 | Msa1344520 | 0.803459 | 3.450420e-49 | -8.615850e-47 |
| Msa0988370 | Msa1344520 | 0.832929 | 7.139169e-56 | -8.615850e-47 |
| Msa0989060 | Msa1344520 | 0.859385 | 4.290037e-63 | -8.615850e-47 |
| Msa1016280 | Msa1344520 | 0.818467 | 1.930205e-52 | -8.615850e-47 |
| Msa1023050 | Msa1344520 | 0.803076 | 4.143445e-49 | -8.615850e-47 |
| Msa1025360 | Msa1344520 | 0.847591 | 1.049792e-59 | -8.615850e-47 |
| Msa1025370 | Msa1344520 | 0.834199 | 3.441520e-56 | -8.615850e-47 |
| Msa1044830 | Msa1344520 | 0.840529 | 8.231618e-58 | -8.615850e-47 |
| Msa1044840 | Msa1344520 | 0.848306 | 6.668228e-60 | -8.615850e-47 |
| Msa1092990 | Msa1344520 | 0.812202 | 4.773669e-51 | -8.615850e-47 |
| Msa1100980 | Msa1344520 | 0.825890 | 3.662019e-54 | -8.615850e-47 |
| Msa0267000 | Msa1344520 | 0.833752 | 4.452120e-56 | -8.615850e-47 |
| Msa0267010 | Msa1344520 | 0.841203 | 5.479244e-58 | -8.615850e-47 |
| Msa0270520 | Msa1344520 | 0.825168 | 5.430307e-54 | -8.615850e-47 |
| Msa0306460 | Msa1344520 | 0.803678 | 3.108897e-49 | -8.615850e-47 |
| Msa0342240 | Msa1344520 | 0.819684 | 1.020632e-52 | -8.615850e-47 |
| Msa0342750 | Msa1344520 | 0.815137 | 1.078676e-51 | -8.615850e-47 |
| Msa0360510 | Msa1344520 | 0.834976 | 2.194223e-56 | -8.615850e-47 |
| Msa0362420 | Msa1344520 | 0.803349 | 3.637385e-49 | -8.615850e-47 |
| Msa1109440 | Msa1344520 | 0.823896 | 1.082307e-53 | -8.615850e-47 |
| Msa1111150 | Msa1344520 | 0.825349 | 4.920384e-54 | -8.615850e-47 |
| Msa1121200 | Msa1344520 | 0.810559 | 1.085422e-50 | -8.615850e-47 |
| Msa1121480 | Msa1344520 | 0.814394 | 1.575560e-51 | -8.615850e-47 |
| Msa1121490 | Msa1344520 | 0.809395 | 1.932567e-50 | -8.615850e-47 |
| Msa1135220 | Msa1344520 | 0.811000 | 8.714133e-51 | -8.615850e-47 |
| Msa1135740 | Msa1344520 | 0.804266 | 2.345511e-49 | -8.615850e-47 |
| Msa1146110 | Msa1344520 | 0.800910 | 1.154419e-48 | -8.615850e-47 |
| Msa1150370 | Msa1344520 | 0.810587 | 1.070101e-50 | -8.615850e-47 |
| Msa1192640 | Msa1344520 | 0.826856 | 2.156349e-54 | -8.615850e-47 |
| Msa0491590 | Msa1344520 | 0.813285 | 2.765908e-51 | -8.615850e-47 |
| Msa0502760 | Msa1344520 | 0.800365 | 1.491261e-48 | -8.615850e-47 |
| Msa0564890 | Msa1344520 | 0.830838 | 2.343306e-55 | -8.615850e-47 |
| Msa0596150 | Msa1344520 | 0.834805 | 2.423058e-56 | -8.615850e-47 |
| Msa0598780 | Msa1344520 | 0.802559 | 5.297289e-49 | -8.615850e-47 |
| Msa0846210 | Msa1344520 | 0.837581 | 4.775806e-57 | -8.615850e-47 |
| Msa0848960 | Msa1344520 | 0.827681 | 1.367816e-54 | -8.615850e-47 |
| Msa0858980 | Msa1344520 | 0.869768 | 2.417708e-66 | -8.615850e-47 |
| Msa0864070 | Msa1344520 | 0.814605 | 1.414852e-51 | -8.615850e-47 |
| Msa0865320 | Msa1344520 | 0.802295 | 6.003924e-49 | -8.615850e-47 |
| Msa0888370 | Msa1344520 | 0.820568 | 6.403540e-53 | -8.615850e-47 |
| Msa0899430 | Msa1344520 | 0.847476 | 1.129090e-59 | -8.615850e-47 |
| Msa0899450 | Msa1344520 | 0.819010 | 1.453504e-52 | -8.615850e-47 |
| Msa0901070 | Msa1344520 | 0.806692 | 7.270315e-50 | -8.615850e-47 |
| Msa0907260 | Msa1344520 | 0.822989 | 1.763492e-53 | -8.615850e-47 |
| Msa0914760 | Msa1344520 | 0.805391 | 1.365566e-49 | -8.615850e-47 |
| Msa0927380 | Msa1344520 | 0.808462 | 3.061481e-50 | -8.615850e-47 |
| Msa0952720 | Msa1344520 | 0.812724 | 3.670823e-51 | -8.615850e-47 |
| Msa0954910 | Msa1344520 | 0.809752 | 1.619577e-50 | -8.615850e-47 |
| Msa0956270 | Msa1344520 | 0.811585 | 6.504542e-51 | -8.615850e-47 |
| Msa0958550 | Msa1344520 | 0.838479 | 2.806784e-57 | -8.615850e-47 |
| Msa0967280 | Msa1344520 | 0.819521 | 1.111847e-52 | -8.615850e-47 |
| Msa0976670 | Msa1344520 | 0.804083 | 2.560672e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344520 | MtrunA17_Chr8g0388651 | 64.789 | 71 | 11 | 1 | 1 | 57 | 81 | 151 | 4.78e-23 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGAGATTTGGTGGGGT+GGG | 0.292846 | tig0006808:+14117 | Msa1344520:CDS |
| TTGGTTTAGAGGGGAAGATT+TGG | 0.314646 | tig0006808:+14033 | Msa1344520:CDS |
| TGGCTTGCGTTTCATCAATA+TGG | 0.322150 | tig0006808:+14156 | Msa1344520:CDS |
| GGTGGAGATTTGGTGGGGTG+GGG | 0.322711 | tig0006808:+14118 | Msa1344520:CDS |
| AAGGTGTTGTGGTGGAGATT+TGG | 0.332973 | tig0006808:+14108 | Msa1344520:CDS |
| GTGGTGGAGATTTGGTGGGG+TGG | 0.361157 | tig0006808:+14116 | Msa1344520:CDS |
| AACAATAACATCATCGCCTT+CGG | 0.383549 | tig0006808:-14059 | None:intergenic |
| GTGTTGTGGTGGAGATTTGG+TGG | 0.392986 | tig0006808:+14111 | Msa1344520:CDS |
| GGTGAAATATGTTGGTTTAG+AGG | 0.411060 | tig0006808:+14022 | Msa1344520:CDS |
| ACCGATGTTCGTCACGCTTC+CGG | 0.415334 | tig0006808:+14001 | None:intergenic |
| TTGAAAGCGCTTATTGTTGC+GGG | 0.436723 | tig0006808:+14080 | Msa1344520:CDS |
| GTGAAATATGTTGGTTTAGA+GGG | 0.447908 | tig0006808:+14023 | Msa1344520:CDS |
| TGTTGTGGTGGAGATTTGGT+GGG | 0.469843 | tig0006808:+14112 | Msa1344520:CDS |
| AGCGCTTATTGTTGCGGGTG+TGG | 0.495782 | tig0006808:+14085 | Msa1344520:CDS |
| TGCGGGTGTGGAAGGTGTTG+TGG | 0.503992 | tig0006808:+14097 | Msa1344520:CDS |
| ACGCTTCCGGTGAAATATGT+TGG | 0.508650 | tig0006808:+14014 | Msa1344520:CDS |
| GTTTAGAGGGGAAGATTTGG+AGG | 0.510688 | tig0006808:+14036 | Msa1344520:CDS |
| CTTATTGTTGCGGGTGTGGA+AGG | 0.549604 | tig0006808:+14089 | Msa1344520:CDS |
| GTTGAAAGCGCTTATTGTTG+CGG | 0.554806 | tig0006808:+14079 | Msa1344520:CDS |
| TTTCATCAATATGGCACTGA+TGG | 0.565296 | tig0006808:+14165 | Msa1344520:CDS |
| ACCGGAAGCGTGACGAACAT+CGG | 0.569489 | tig0006808:-14002 | None:intergenic |
| GTTGTGGTGGAGATTTGGTG+GGG | 0.574564 | tig0006808:+14113 | Msa1344520:CDS |
| TCTAAACCAACATATTTCAC+CGG | 0.601316 | tig0006808:-14020 | None:intergenic |
| TGGGGTTGAACTTGTTACGA+TGG | 0.608700 | tig0006808:+14136 | Msa1344520:CDS |
| TGAAATATGTTGGTTTAGAG+GGG | 0.610207 | tig0006808:+14024 | Msa1344520:CDS |
| GGGGAAGATTTGGAGGCCGA+AGG | 0.616896 | tig0006808:+14043 | Msa1344520:CDS |
| GGGTGTGGAAGGTGTTGTGG+TGG | 0.643276 | tig0006808:+14100 | Msa1344520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTAAACCAACATATTTCAC+CGG | - | tig0006808:14023-14042 | None:intergenic | 30.0% | |
| !! | GTGAAATATGTTGGTTTAGA+GGG | + | tig0006808:14023-14042 | Msa1344520:CDS | 30.0% |
| !! | TGAAATATGTTGGTTTAGAG+GGG | + | tig0006808:14024-14043 | Msa1344520:CDS | 30.0% |
| !! | GGTGAAATATGTTGGTTTAG+AGG | + | tig0006808:14022-14041 | Msa1344520:CDS | 35.0% |
| AACAATAACATCATCGCCTT+CGG | - | tig0006808:14062-14081 | None:intergenic | 35.0% | |
| TTGGTTTAGAGGGGAAGATT+TGG | + | tig0006808:14033-14052 | Msa1344520:CDS | 40.0% | |
| !! | GTTGAAAGCGCTTATTGTTG+CGG | + | tig0006808:14079-14098 | Msa1344520:CDS | 40.0% |
| !! | TTGAAAGCGCTTATTGTTGC+GGG | + | tig0006808:14080-14099 | Msa1344520:CDS | 40.0% |
| ! | TGGCTTGCGTTTCATCAATA+TGG | + | tig0006808:14156-14175 | Msa1344520:CDS | 40.0% |
| ACGCTTCCGGTGAAATATGT+TGG | + | tig0006808:14014-14033 | Msa1344520:CDS | 45.0% | |
| GTTTAGAGGGGAAGATTTGG+AGG | + | tig0006808:14036-14055 | Msa1344520:CDS | 45.0% | |
| !! | AAGGTGTTGTGGTGGAGATT+TGG | + | tig0006808:14108-14127 | Msa1344520:CDS | 45.0% |
| ! | TGTTGTGGTGGAGATTTGGT+GGG | + | tig0006808:14112-14131 | Msa1344520:CDS | 45.0% |
| !! | TGGGGTTGAACTTGTTACGA+TGG | + | tig0006808:14136-14155 | Msa1344520:CDS | 45.0% |
| CTTATTGTTGCGGGTGTGGA+AGG | + | tig0006808:14089-14108 | Msa1344520:CDS | 50.0% | |
| ! | GTGTTGTGGTGGAGATTTGG+TGG | + | tig0006808:14111-14130 | Msa1344520:CDS | 50.0% |
| !! | GTTGTGGTGGAGATTTGGTG+GGG | + | tig0006808:14113-14132 | Msa1344520:CDS | 50.0% |
| AGCGCTTATTGTTGCGGGTG+TGG | + | tig0006808:14085-14104 | Msa1344520:CDS | 55.0% | |
| !! | TGGTGGAGATTTGGTGGGGT+GGG | + | tig0006808:14117-14136 | Msa1344520:CDS | 55.0% |
| GGGGAAGATTTGGAGGCCGA+AGG | + | tig0006808:14043-14062 | Msa1344520:CDS | 60.0% | |
| !! | TGCGGGTGTGGAAGGTGTTG+TGG | + | tig0006808:14097-14116 | Msa1344520:CDS | 60.0% |
| !! | GGGTGTGGAAGGTGTTGTGG+TGG | + | tig0006808:14100-14119 | Msa1344520:CDS | 60.0% |
| !! | GTGGTGGAGATTTGGTGGGG+TGG | + | tig0006808:14116-14135 | Msa1344520:CDS | 60.0% |
| !! | GGTGGAGATTTGGTGGGGTG+GGG | + | tig0006808:14118-14137 | Msa1344520:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0006808 | gene | 14005 | 14184 | 14005 | ID=Msa1344520;Name=Msa1344520 |
| tig0006808 | mRNA | 14005 | 14184 | 14005 | ID=Msa1344520-mRNA-1;Parent=Msa1344520;Name=Msa1344520-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|-1|0|1|-1|1|1|0|59 |
| tig0006808 | exon | 14005 | 14184 | 14005 | ID=Msa1344520-mRNA-1:exon:4581;Parent=Msa1344520-mRNA-1 |
| tig0006808 | CDS | 14005 | 14184 | 14005 | ID=Msa1344520-mRNA-1:cds;Parent=Msa1344520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
