AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1008080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1008080 MtrunA17_Chr6g0454031 81.600 250 44 1 1 250 1 248 7.96e-159 440
Msa1008080 MtrunA17_Chr7g0218381 80.800 250 0 1 1 250 1 202 9.41e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1008080 AT5G39360.1 76.494 251 56 2 1 250 1 249 1.61e-148 414
Msa1008080 AT5G15440.1 76.423 246 57 1 1 246 1 245 9.89e-144 404
Msa1008080 AT3G63060.1 42.534 221 125 2 23 242 36 255 3.90e-63 199

Find 127 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAATATTCAACTAAA+AGG 0.164816 7_1:-8018085 None:intergenic
TAGTGTTTCATAATTTAAAA+TGG 0.209786 7_1:+8018400 Msa1008080:CDS
CACAACTTCGCTTTACAATA+AGG 0.238798 7_1:-8018885 None:intergenic
AACGATAATGCGACTTTCAT+TGG 0.271652 7_1:+8017151 Msa1008080:five_prime_UTR
CAAGGTGAAGAAGGAGATAT+TGG 0.288349 7_1:+8018768 Msa1008080:CDS
TTCAACTATTACTCCAATTC+AGG 0.290810 7_1:-8019053 None:intergenic
CAGCCCAAACCCATCATTAA+TGG 0.299946 7_1:-8017350 None:intergenic
GATGGGAACTGGAGGGCATT+AGG 0.301055 7_1:+8018564 Msa1008080:CDS
AGGCAAATGGTGAGCCTCTT+GGG 0.305235 7_1:+8020211 Msa1008080:three_prime_UTR
TGCAGATCACACAACATCTT+TGG 0.305769 7_1:-8018519 None:intergenic
CAGATTATTTACAAGTTGTT+TGG 0.310423 7_1:-8020602 None:intergenic
AAGACTGTTTGTGGGGCTTA+TGG 0.311944 7_1:+8017409 Msa1008080:five_prime_UTR
ACTTATTTCCATACGCTTGC+AGG 0.313651 7_1:+8017465 Msa1008080:five_prime_UTR
AGCTAATATGAGGGGCAATT+TGG 0.322687 7_1:-8016370 None:intergenic
TTGTGAATATTTGAACTCTT+AGG 0.332759 7_1:+8017655 Msa1008080:five_prime_UTR
CAGAATCAGACAACGGCAAT+AGG 0.334261 7_1:-8019022 None:intergenic
TTCTGAAGAGGCGCCTGAAT+TGG 0.352655 7_1:+8019040 Msa1008080:CDS
ACATCCATTAATGATGGGTT+TGG 0.356850 7_1:+8017346 Msa1008080:five_prime_UTR
CTCTATCATGTGTGTGCAAA+TGG 0.361648 7_1:+8018442 Msa1008080:CDS
TACAATATGTTTGGTTTGGT+TGG 0.362283 7_1:-8017899 None:intergenic
CTACAAATTTACAATATGTT+TGG 0.363437 7_1:-8017908 None:intergenic
ACGATTATTTACATCATGTT+TGG 0.365570 7_1:-8020563 None:intergenic
TGCTTCCAGCAGTGTGTTGT+GGG 0.366169 7_1:-8018009 None:intergenic
CAAAGTGATTGAGTATTGTT+TGG 0.369583 7_1:+8017744 Msa1008080:five_prime_UTR
AATGTCTGGTTGGTATGCTT+TGG 0.390732 7_1:+8020413 Msa1008080:three_prime_UTR
ACAACTTCGCTTTACAATAA+GGG 0.398128 7_1:-8018884 None:intergenic
TGCCTCAATGGTCACTTGAT+TGG 0.401501 7_1:+8018990 Msa1008080:CDS
CAAAGTGCTAGCTGCAGGTT+GGG 0.401792 7_1:+8018936 Msa1008080:CDS
TGTATGTTGCTGCTAAAATT+AGG 0.405550 7_1:+8017708 Msa1008080:five_prime_UTR
AATACAACACCTCCAATAGA+TGG 0.406390 7_1:-8020358 None:intergenic
AATTTACAATATGTTTGGTT+TGG 0.410012 7_1:-8017903 None:intergenic
GCCACGTCGTTGATGGGAAC+TGG 0.411747 7_1:+8018553 Msa1008080:CDS
TTGTGTGATCTGCAATCAAC+TGG 0.417058 7_1:+8018528 Msa1008080:CDS
AAGCTTCTTACGTACTGTTC+GGG 0.422997 7_1:+8018588 Msa1008080:CDS
ACTGGGAGCCACGTCGTTGA+TGG 0.424116 7_1:+8018546 Msa1008080:CDS
CATCCATTAATGATGGGTTT+GGG 0.424733 7_1:+8017347 Msa1008080:five_prime_UTR
ATTGGGTTTCTAACTCCGGC+TGG 0.427801 7_1:+8017169 Msa1008080:five_prime_UTR
ATGGGAACTGGAGGGCATTA+GGG 0.429718 7_1:+8018565 Msa1008080:CDS
ATCTTCACTTAGATGCCCTT+TGG 0.429745 7_1:-8018367 None:intergenic
GAAGCTTCTTACGTACTGTT+CGG 0.432478 7_1:+8018587 Msa1008080:CDS
CTAAACACATCCATTAATGA+TGG 0.436220 7_1:+8017340 Msa1008080:five_prime_UTR
TTCTGTAATTATACTTGTGA+TGG 0.437144 7_1:+8017845 Msa1008080:five_prime_UTR
ACGATAATGCGACTTTCATT+GGG 0.438422 7_1:+8017152 Msa1008080:five_prime_UTR
AACCTGCAGCTAGCACTTTG+AGG 0.444997 7_1:-8018933 None:intergenic
TTGTACCGATAGCAATTCCT+TGG 0.446337 7_1:+8017295 Msa1008080:five_prime_UTR
TGATATCTTTCCTCTAATGA+AGG 0.451969 7_1:+8017264 Msa1008080:five_prime_UTR
CTGCTTCCAGCAGTGTGTTG+TGG 0.455287 7_1:-8018010 None:intergenic
GCCTCAATGGTCACTTGATT+GGG 0.456870 7_1:+8018991 Msa1008080:CDS
CATCTAGATCAAGGTGAAGA+AGG 0.463015 7_1:+8018759 Msa1008080:CDS
AAGGCAAATGGTGAGCCTCT+TGG 0.463223 7_1:+8020210 Msa1008080:three_prime_UTR
CTCTTTCCATAGAACTCGCT+TGG 0.463797 7_1:-8018478 None:intergenic
GCTTGATGTGCAACGTCAAA+CGG 0.468341 7_1:-8017111 None:intergenic
AAAACACCGTCCTTCATTAG+AGG 0.473973 7_1:-8017274 None:intergenic
ATCTTGCCAAGCGAGTTCTA+TGG 0.476286 7_1:+8018472 Msa1008080:CDS
AAATTAATATTCAACTAAAA+GGG 0.477316 7_1:-8018084 None:intergenic
CATTAATGATGGGTTTGGGC+TGG 0.477597 7_1:+8017351 Msa1008080:five_prime_UTR
ATGTAGCATGATCCATCTAT+TGG 0.479951 7_1:+8020346 Msa1008080:three_prime_UTR
CATCTTGAATTTAGCAGAGT+CGG 0.482579 7_1:-8020184 None:intergenic
AACTTGTGTAAGACTGTTTG+TGG 0.486725 7_1:+8017400 Msa1008080:five_prime_UTR
TCAAAGTGCTAGCTGCAGGT+TGG 0.489268 7_1:+8018935 Msa1008080:CDS
TGTACCGATAGCAATTCCTT+GGG 0.492322 7_1:+8017296 Msa1008080:five_prime_UTR
AGAATCAGACAACGGCAATA+GGG 0.497429 7_1:-8019021 None:intergenic
CAAACAATACTCAATCACTT+TGG 0.501113 7_1:-8017743 None:intergenic
TTCAAGATGAAGAAGGCAAA+TGG 0.503851 7_1:+8020198 Msa1008080:three_prime_UTR
TGAAACCCACAACACACTGC+TGG 0.504466 7_1:+8018004 Msa1008080:five_prime_UTR
CTGGGAGCCACGTCGTTGAT+GGG 0.504476 7_1:+8018547 Msa1008080:CDS
TTTCATTGGGTTTCTAACTC+CGG 0.505320 7_1:+8017165 Msa1008080:five_prime_UTR
AAATTGCCCCTCATATTAGC+TGG 0.506482 7_1:+8016372 Msa1008080:five_prime_UTR
GTGAAGAATACTCATAGTAT+AGG 0.509863 7_1:+8020308 Msa1008080:three_prime_UTR
TATGTAAGAACACTTATGCT+TGG 0.512763 7_1:+8020520 Msa1008080:three_prime_UTR
GATAGAGCAGCAATCAGCTT+AGG 0.512808 7_1:-8018426 None:intergenic
TAAACACATCCATTAATGAT+GGG 0.518097 7_1:+8017341 Msa1008080:five_prime_UTR
TTTGACGTTGCACATCAAGC+AGG 0.518359 7_1:+8017114 Msa1008080:five_prime_UTR
TTATTTACAAGTTGTTTGGA+TGG 0.527757 7_1:-8020598 None:intergenic
AGAAAGATGCTTATTAGTAA+AGG 0.529828 7_1:+8018834 Msa1008080:CDS
AACTTGTAAATAATCTGACT+TGG 0.531224 7_1:+8020608 Msa1008080:three_prime_UTR
TTATTTACATCATGTTTGGA+TGG 0.531853 7_1:-8020559 None:intergenic
CTTCAATTTCCACTCAGAGG+TGG 0.535038 7_1:+8016457 Msa1008080:five_prime_UTR
ACAAACTATACTGATTATGT+TGG 0.537557 7_1:+8020631 Msa1008080:three_prime_UTR
TTCCTCAAAGTGCTAGCTGC+AGG 0.540338 7_1:+8018931 Msa1008080:CDS
TTAACGATCCTGCAAGCGTA+TGG 0.541472 7_1:-8017473 None:intergenic
TTCAATTTCCACTCAGAGGT+GGG 0.542737 7_1:+8016458 Msa1008080:five_prime_UTR
AAATGGTGAGCCTCTTGGGG+TGG 0.542820 7_1:+8020215 Msa1008080:three_prime_UTR
TGCTAAATTCAAGATGAAGA+AGG 0.543882 7_1:+8020191 Msa1008080:three_prime_UTR
ACTAAAAGGGTTGCAATCCA+TGG 0.544566 7_1:-8018071 None:intergenic
AACTCAACACTTCTCCCAGC+CGG 0.547403 7_1:-8017184 None:intergenic
GCCGTTGTCTGATTCTGAAG+AGG 0.548453 7_1:+8019028 Msa1008080:CDS
AACATTCAGAATCAGATCCC+TGG 0.551777 7_1:+8018636 Msa1008080:CDS
TTGGGTTTCTAACTCCGGCT+GGG 0.554045 7_1:+8017170 Msa1008080:five_prime_UTR
TAGATGCAGATCACTTGTGC+AGG 0.554067 7_1:+8020286 Msa1008080:three_prime_UTR
CCTTGTGAGCATCTAGATCA+AGG 0.554478 7_1:+8018750 Msa1008080:CDS
ACTTGTGTAAGACTGTTTGT+GGG 0.554723 7_1:+8017401 Msa1008080:five_prime_UTR
GTCTGATACACAAAGTGACC+AGG 0.560165 7_1:-8018654 None:intergenic
CTAGCTGTCAATGTTCCAAA+GGG 0.560254 7_1:+8018352 Msa1008080:CDS
CCTTGATCTAGATGCTCACA+AGG 0.564697 7_1:-8018750 None:intergenic
TGATGTGCTATCGCGTATGT+TGG 0.566092 7_1:+8020242 Msa1008080:three_prime_UTR
CAATTTGGTCAAGAAGAGTA+AGG 0.567116 7_1:-8016355 None:intergenic
TTCTTGGTTAGCAAACATCA+TGG 0.570595 7_1:-8018245 None:intergenic
GCTAGCTGTCAATGTTCCAA+AGG 0.578026 7_1:+8018351 Msa1008080:CDS
TAAGTCGTTTGCAACGTCAA+AGG 0.578884 7_1:+8018809 Msa1008080:CDS
TTAAGCAGTTTATCTAAACA+TGG 0.585305 7_1:+8017609 Msa1008080:five_prime_UTR
CGTTACACTGAACAACAAGC+TGG 0.585680 7_1:-8017060 None:intergenic
TTGACGTTGCACATCAAGCA+GGG 0.587625 7_1:+8017115 Msa1008080:five_prime_UTR
TAGCATGATCCATCTATTGG+AGG 0.588497 7_1:+8020349 Msa1008080:three_prime_UTR
ATCTTTCCTCTAATGAAGGA+CGG 0.589704 7_1:+8017268 Msa1008080:five_prime_UTR
CTTATTGTAAAGCGAAGTTG+TGG 0.591906 7_1:+8018886 Msa1008080:CDS
ACGTCGTTGATGGGAACTGG+AGG 0.594238 7_1:+8018556 Msa1008080:CDS
TACTCATAGTATAGGTGACT+AGG 0.595516 7_1:+8020316 Msa1008080:three_prime_UTR
TATACCCAAGGAATTGCTAT+CGG 0.597827 7_1:-8017300 None:intergenic
TTTGTAAGCACGATATCATG+AGG 0.601873 7_1:-8020682 None:intergenic
TAACTTCAATTTCCACTCAG+AGG 0.610940 7_1:+8016454 Msa1008080:five_prime_UTR
AAAACATCAGTTCTGCATTG+TGG 0.613771 7_1:-8018714 None:intergenic
GGCAAATGGTGAGCCTCTTG+GGG 0.617357 7_1:+8020212 Msa1008080:three_prime_UTR
AGCTAGCTGAATGTATGCAT+CGG 0.619048 7_1:-8018335 None:intergenic
TGTTCGGGATGCACGAAAGG+TGG 0.621782 7_1:+8018603 Msa1008080:CDS
CTTGTGTAAGACTGTTTGTG+GGG 0.622230 7_1:+8017402 Msa1008080:five_prime_UTR
GGAGATATTGGATTCTTCCG+CGG 0.632203 7_1:+8018780 Msa1008080:CDS
ACATCATAAACCACCCCAAG+AGG 0.632395 7_1:-8020225 None:intergenic
CACATGAGGGAAATAAAACA+AGG 0.636730 7_1:+8017816 Msa1008080:five_prime_UTR
TCCAGTTCCCATCAACGACG+TGG 0.656887 7_1:-8018554 None:intergenic
CGTCGTTGATGGGAACTGGA+GGG 0.667520 7_1:+8018557 Msa1008080:CDS
TCCCAATCAAGTGACCATTG+AGG 0.669913 7_1:-8018992 None:intergenic
GCCTCTTCAGAATCAGACAA+CGG 0.682457 7_1:-8019029 None:intergenic
GAACTGGTTTGTGAGTACCA+TGG 0.688393 7_1:+8018054 Msa1008080:five_prime_UTR
TCTGATACACAAAGTGACCA+GGG 0.703260 7_1:-8018653 None:intergenic
TGTGTGATCTGCAATCAACT+GGG 0.707515 7_1:+8018529 Msa1008080:CDS
GTGCAACGTCAAACGGCATG+TGG 0.713160 7_1:-8017104 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATATTCAACTAAAA+GGG - chr7_1:8018087-8018106 None:intergenic 10.0%
!! AAAATTAATATTCAACTAAA+AGG - chr7_1:8018088-8018107 None:intergenic 10.0%
!! TATCAGTTTATAAAAATTCT+TGG - chr7_1:8018264-8018283 None:intergenic 15.0%
!! TAGTGTTTCATAATTTAAAA+TGG + chr7_1:8018400-8018419 Msa1008080:CDS 15.0%
!!! TTTATTTTTTATTTCGTGTA+AGG + chr7_1:8019816-8019835 Msa1008080:intron 15.0%
!!! TGATTTTATTCGATTTGTAT+TGG + chr7_1:8016544-8016563 Msa1008080:intron 20.0%
!!! TTGATTTGCATATAATTGAT+GGG + chr7_1:8016612-8016631 Msa1008080:intron 20.0%
!!! AAAGATATTTTTGTAGAAGA+GGG + chr7_1:8017220-8017239 Msa1008080:five_prime_UTR 20.0%
!!! TAATATTTTAAGAGCTTGTT+TGG + chr7_1:8017778-8017797 Msa1008080:five_prime_UTR 20.0%
!!! AATATTTTAAGAGCTTGTTT+GGG + chr7_1:8017779-8017798 Msa1008080:five_prime_UTR 20.0%
!! AATTTACAATATGTTTGGTT+TGG - chr7_1:8017906-8017925 None:intergenic 20.0%
!! CTACAAATTTACAATATGTT+TGG - chr7_1:8017911-8017930 None:intergenic 20.0%
!!! TGTTTTGCTTAAGTATATAT+TGG + chr7_1:8018107-8018126 Msa1008080:five_prime_UTR 20.0%
!!! ATTTTTCTTTTAGTTTTGCT+AGG + chr7_1:8018284-8018303 Msa1008080:five_prime_UTR 20.0%
!! AACAGAAAAATAGTAAGTAA+TGG - chr7_1:8019111-8019130 None:intergenic 20.0%
!! ATAATCTATGATTTCTTTCT+AGG + chr7_1:8019231-8019250 Msa1008080:intron 20.0%
!! TTTAGACCTAATAATTGATA+GGG - chr7_1:8019290-8019309 None:intergenic 20.0%
!! AAAAGATAGTTAGAAGTAAA+TGG - chr7_1:8019319-8019338 None:intergenic 20.0%
!!! TTTAAAACATGTCTTTTAGA+GGG - chr7_1:8019501-8019520 None:intergenic 20.0%
!!! ATTTAAAACATGTCTTTTAG+AGG - chr7_1:8019502-8019521 None:intergenic 20.0%
!!! ATTTGCTAGTTATATATGTA+AGG + chr7_1:8020015-8020034 Msa1008080:intron 20.0%
!!! ATGTGATTAAATTAGTTTGA+AGG + chr7_1:8020113-8020132 Msa1008080:intron 20.0%
!!! ATTCTATTATTTTCAATGTC+TGG + chr7_1:8020399-8020418 Msa1008080:three_prime_UTR 20.0%
!!! CTTGATTTGCATATAATTGA+TGG + chr7_1:8016611-8016630 Msa1008080:intron 25.0%
! TATAATTGATGGGAAATTTC+TGG + chr7_1:8016622-8016641 Msa1008080:intron 25.0%
! ATAATTGATGGGAAATTTCT+GGG + chr7_1:8016623-8016642 Msa1008080:intron 25.0%
!!! CAAAGATATTTTTGTAGAAG+AGG + chr7_1:8017219-8017238 Msa1008080:five_prime_UTR 25.0%
! TGATTACAAAAATATACCCA+AGG - chr7_1:8017315-8017334 None:intergenic 25.0%
! TAAACACATCCATTAATGAT+GGG + chr7_1:8017341-8017360 Msa1008080:five_prime_UTR 25.0%
! TTAAGCAGTTTATCTAAACA+TGG + chr7_1:8017609-8017628 Msa1008080:five_prime_UTR 25.0%
! TTGTGAATATTTGAACTCTT+AGG + chr7_1:8017655-8017674 Msa1008080:five_prime_UTR 25.0%
!!! ATATTTTAAGAGCTTGTTTG+GGG + chr7_1:8017780-8017799 Msa1008080:five_prime_UTR 25.0%
!! ACAAAATTTTGAACACATGA+GGG + chr7_1:8017803-8017822 Msa1008080:five_prime_UTR 25.0%
! TTCTGTAATTATACTTGTGA+TGG + chr7_1:8017845-8017864 Msa1008080:five_prime_UTR 25.0%
!! GTAGTCTTCATTTTCATTTT+TGG + chr7_1:8017927-8017946 Msa1008080:five_prime_UTR 25.0%
!!! TCATTTTTGTTTTCTTGAAC+TGG + chr7_1:8018038-8018057 Msa1008080:five_prime_UTR 25.0%
! AGAAAGATGCTTATTAGTAA+AGG + chr7_1:8018834-8018853 Msa1008080:CDS 25.0%
!! GTAATAGTTGAATGATTTTC+AGG + chr7_1:8019064-8019083 Msa1008080:exon 25.0%
! GTAAATCCCTATCAATTATT+AGG + chr7_1:8019281-8019300 Msa1008080:intron 25.0%
! CTTTAGACCTAATAATTGAT+AGG - chr7_1:8019291-8019310 None:intergenic 25.0%
!! CTAACTATCTTTTTCTGATT+TGG + chr7_1:8019326-8019345 Msa1008080:intron 25.0%
!! AAAATCAAAGACAAGAGTAT+AGG - chr7_1:8019373-8019392 None:intergenic 25.0%
! AATCATTAGAGAGAAAAAGA+TGG - chr7_1:8019576-8019595 None:intergenic 25.0%
! AAATTGTATGTTGAATTCAG+TGG + chr7_1:8019747-8019766 Msa1008080:intron 25.0%
!! CTCATTAGATATTTTGCAAA+TGG + chr7_1:8019863-8019882 Msa1008080:intron 25.0%
! AATTACTATAATTGCACTAG+TGG + chr7_1:8019898-8019917 Msa1008080:intron 25.0%
! AGGATGTATCTGATTATATT+TGG + chr7_1:8020035-8020054 Msa1008080:intron 25.0%
!! TATTATTTTCAATGTCTGGT+TGG + chr7_1:8020403-8020422 Msa1008080:three_prime_UTR 25.0%
! TTATTTACATCATGTTTGGA+TGG - chr7_1:8020562-8020581 None:intergenic 25.0%
! ACGATTATTTACATCATGTT+TGG - chr7_1:8020566-8020585 None:intergenic 25.0%
! TTATTTACAAGTTGTTTGGA+TGG - chr7_1:8020601-8020620 None:intergenic 25.0%
! CAGATTATTTACAAGTTGTT+TGG - chr7_1:8020605-8020624 None:intergenic 25.0%
! AACTTGTAAATAATCTGACT+TGG + chr7_1:8020608-8020627 Msa1008080:three_prime_UTR 25.0%
!! ACAAACTATACTGATTATGT+TGG + chr7_1:8020631-8020650 Msa1008080:three_prime_UTR 25.0%
! AATTTTCCAGCTAATATGAG+GGG - chr7_1:8016381-8016400 None:intergenic 30.0%
! CAATTTTCCAGCTAATATGA+GGG - chr7_1:8016382-8016401 None:intergenic 30.0%
TTATAGCTAGCTGATAACTT+TGG + chr7_1:8016772-8016791 Msa1008080:intron 30.0%
ATCAGCAGTCAATTAATTGA+TGG + chr7_1:8016818-8016837 Msa1008080:intron 30.0%
TCAGCAGTCAATTAATTGAT+GGG + chr7_1:8016819-8016838 Msa1008080:intron 30.0%
TGATATCTTTCCTCTAATGA+AGG + chr7_1:8017264-8017283 Msa1008080:five_prime_UTR 30.0%
CTAAACACATCCATTAATGA+TGG + chr7_1:8017340-8017359 Msa1008080:five_prime_UTR 30.0%
! TGGCTTTTATGTATGATTTG+AGG + chr7_1:8017629-8017648 Msa1008080:five_prime_UTR 30.0%
! GGCTTTTATGTATGATTTGA+GGG + chr7_1:8017630-8017649 Msa1008080:five_prime_UTR 30.0%
TGTATGTTGCTGCTAAAATT+AGG + chr7_1:8017708-8017727 Msa1008080:five_prime_UTR 30.0%
CAAACAATACTCAATCACTT+TGG - chr7_1:8017746-8017765 None:intergenic 30.0%
! CAAAGTGATTGAGTATTGTT+TGG + chr7_1:8017744-8017763 Msa1008080:five_prime_UTR 30.0%
! GACAAAATTTTGAACACATG+AGG + chr7_1:8017802-8017821 Msa1008080:five_prime_UTR 30.0%
!! TACAATATGTTTGGTTTGGT+TGG - chr7_1:8017902-8017921 None:intergenic 30.0%
!! GTGTTTGAGTTTTGTCTATT+TGG + chr7_1:8018216-8018235 Msa1008080:five_prime_UTR 30.0%
ACAACTTCGCTTTACAATAA+GGG - chr7_1:8018887-8018906 None:intergenic 30.0%
TTCAACTATTACTCCAATTC+AGG - chr7_1:8019056-8019075 None:intergenic 30.0%
!! TTCAGGTATTTTTGTGTATC+TGG + chr7_1:8019081-8019100 Msa1008080:intron 30.0%
!! CTTTACTTTTCTTTGAGAAC+TGG + chr7_1:8019134-8019153 Msa1008080:intron 30.0%
TCTATGATTTCTTTCTAGGT+AGG + chr7_1:8019235-8019254 Msa1008080:intron 30.0%
CTATGATTTCTTTCTAGGTA+GGG + chr7_1:8019236-8019255 Msa1008080:intron 30.0%
GGAAGACTAAGGAAAAATAT+GGG - chr7_1:8019352-8019371 None:intergenic 30.0%
AGGAAGACTAAGGAAAAATA+TGG - chr7_1:8019353-8019372 None:intergenic 30.0%
!! TCATAGTTTTCTGTTGCTTA+GGG - chr7_1:8019443-8019462 None:intergenic 30.0%
!! TTCATAGTTTTCTGTTGCTT+AGG - chr7_1:8019444-8019463 None:intergenic 30.0%
AATGCTACACAAGATAAGAT+AGG - chr7_1:8019610-8019629 None:intergenic 30.0%
AATATTAGCATGTGATACAC+AGG - chr7_1:8019795-8019814 None:intergenic 30.0%
TGTTCAAAACAGTTTGACAA+TGG - chr7_1:8019944-8019963 None:intergenic 30.0%
AATGATTAGCAACTATCTTC+TGG + chr7_1:8020060-8020079 Msa1008080:intron 30.0%
ATACAATTAACCTGTTACTG+AGG - chr7_1:8020088-8020107 None:intergenic 30.0%
TGCTAAATTCAAGATGAAGA+AGG + chr7_1:8020191-8020210 Msa1008080:three_prime_UTR 30.0%
GTGAAGAATACTCATAGTAT+AGG + chr7_1:8020308-8020327 Msa1008080:three_prime_UTR 30.0%
TATGTAAGAACACTTATGCT+TGG + chr7_1:8020520-8020539 Msa1008080:three_prime_UTR 30.0%
CAATTTGGTCAAGAAGAGTA+AGG - chr7_1:8016358-8016377 None:intergenic 35.0%
! GCAATTTTCCAGCTAATATG+AGG - chr7_1:8016383-8016402 None:intergenic 35.0%
TAACTTCAATTTCCACTCAG+AGG + chr7_1:8016454-8016473 Msa1008080:five_prime_UTR 35.0%
GTTACTGTCATTAAATGTCG+CGG + chr7_1:8016894-8016913 Msa1008080:intron 35.0%
AGCTACAACAGAGAAAAATG+TGG - chr7_1:8017005-8017024 None:intergenic 35.0%
AACGATAATGCGACTTTCAT+TGG + chr7_1:8017151-8017170 Msa1008080:five_prime_UTR 35.0%
ACGATAATGCGACTTTCATT+GGG + chr7_1:8017152-8017171 Msa1008080:five_prime_UTR 35.0%
TTTCATTGGGTTTCTAACTC+CGG + chr7_1:8017165-8017184 Msa1008080:five_prime_UTR 35.0%
ATCTTTCCTCTAATGAAGGA+CGG + chr7_1:8017268-8017287 Msa1008080:five_prime_UTR 35.0%
TATACCCAAGGAATTGCTAT+CGG - chr7_1:8017303-8017322 None:intergenic 35.0%
!! ACATCCATTAATGATGGGTT+TGG + chr7_1:8017346-8017365 Msa1008080:five_prime_UTR 35.0%
!! CATCCATTAATGATGGGTTT+GGG + chr7_1:8017347-8017366 Msa1008080:five_prime_UTR 35.0%
AACTTGTGTAAGACTGTTTG+TGG + chr7_1:8017400-8017419 Msa1008080:five_prime_UTR 35.0%
ACTTGTGTAAGACTGTTTGT+GGG + chr7_1:8017401-8017420 Msa1008080:five_prime_UTR 35.0%
CACATGAGGGAAATAAAACA+AGG + chr7_1:8017816-8017835 Msa1008080:five_prime_UTR 35.0%
TTCTTGGTTAGCAAACATCA+TGG - chr7_1:8018248-8018267 None:intergenic 35.0%
!!! ACTCGATTTTCTAGAACATC+AGG + chr7_1:8018675-8018694 Msa1008080:CDS 35.0%
!!! CTCGATTTTCTAGAACATCA+GGG + chr7_1:8018676-8018695 Msa1008080:CDS 35.0%
AAAACATCAGTTCTGCATTG+TGG - chr7_1:8018717-8018736 None:intergenic 35.0%
CACAACTTCGCTTTACAATA+AGG - chr7_1:8018888-8018907 None:intergenic 35.0%
CTTATTGTAAAGCGAAGTTG+TGG + chr7_1:8018886-8018905 Msa1008080:CDS 35.0%
! GATCCATTTTGAATGTTAGC+AGG + chr7_1:8019156-8019175 Msa1008080:intron 35.0%
CTACCTGCTAACATTCAAAA+TGG - chr7_1:8019162-8019181 None:intergenic 35.0%
! ACAAGAGTATAGGAAGACTA+AGG - chr7_1:8019363-8019382 None:intergenic 35.0%
! GATTTTCTGACTTTTCCACA+TGG + chr7_1:8019387-8019406 Msa1008080:intron 35.0%
!! CATAGTTTTCTGTTGCTTAG+GGG - chr7_1:8019442-8019461 None:intergenic 35.0%
CATCAAGCGAAAAAAAGTTG+AGG - chr7_1:8019527-8019546 None:intergenic 35.0%
AGATGGTACAATCTCATCTT+AGG - chr7_1:8019559-8019578 None:intergenic 35.0%
CATGTGATACACAGGAAAAA+AGG - chr7_1:8019787-8019806 None:intergenic 35.0%
ATTGAGTCAATGGTTAGTGA+TGG + chr7_1:8019839-8019858 Msa1008080:intron 35.0%
AGCAAATAGAGAACAGTAGA+AGG - chr7_1:8020002-8020021 None:intergenic 35.0%
CATCTTGAATTTAGCAGAGT+CGG - chr7_1:8020187-8020206 None:intergenic 35.0%
TTCAAGATGAAGAAGGCAAA+TGG + chr7_1:8020198-8020217 Msa1008080:three_prime_UTR 35.0%
TATCGCGTATGTTGGAAAAA+TGG + chr7_1:8020250-8020269 Msa1008080:three_prime_UTR 35.0%
TACTCATAGTATAGGTGACT+AGG + chr7_1:8020316-8020335 Msa1008080:three_prime_UTR 35.0%
ATGTAGCATGATCCATCTAT+TGG + chr7_1:8020346-8020365 Msa1008080:three_prime_UTR 35.0%
AATACAACACCTCCAATAGA+TGG - chr7_1:8020361-8020380 None:intergenic 35.0%
AGCTAATATGAGGGGCAATT+TGG - chr7_1:8016373-8016392 None:intergenic 40.0%
AAATTGCCCCTCATATTAGC+TGG + chr7_1:8016372-8016391 Msa1008080:five_prime_UTR 40.0%
TTCAATTTCCACTCAGAGGT+GGG + chr7_1:8016458-8016477 Msa1008080:five_prime_UTR 40.0%
TTCTGGGTGATCTATGATCT+TGG + chr7_1:8016639-8016658 Msa1008080:intron 40.0%
TCTGGGTGATCTATGATCTT+GGG + chr7_1:8016640-8016659 Msa1008080:intron 40.0%
AAAACACCGTCCTTCATTAG+AGG - chr7_1:8017277-8017296 None:intergenic 40.0%
! TTGTACCGATAGCAATTCCT+TGG + chr7_1:8017295-8017314 Msa1008080:five_prime_UTR 40.0%
! TGTACCGATAGCAATTCCTT+GGG + chr7_1:8017296-8017315 Msa1008080:five_prime_UTR 40.0%
CTTGTGTAAGACTGTTTGTG+GGG + chr7_1:8017402-8017421 Msa1008080:five_prime_UTR 40.0%
ACTTATTTCCATACGCTTGC+AGG + chr7_1:8017465-8017484 Msa1008080:five_prime_UTR 40.0%
ACTAAAAGGGTTGCAATCCA+TGG - chr7_1:8018074-8018093 None:intergenic 40.0%
AGCTAGCTGAATGTATGCAT+CGG - chr7_1:8018338-8018357 None:intergenic 40.0%
CTAGCTGTCAATGTTCCAAA+GGG + chr7_1:8018352-8018371 Msa1008080:CDS 40.0%
ATCTTCACTTAGATGCCCTT+TGG - chr7_1:8018370-8018389 None:intergenic 40.0%
CTCTATCATGTGTGTGCAAA+TGG + chr7_1:8018442-8018461 Msa1008080:CDS 40.0%
TGCAGATCACACAACATCTT+TGG - chr7_1:8018522-8018541 None:intergenic 40.0%
TTGTGTGATCTGCAATCAAC+TGG + chr7_1:8018528-8018547 Msa1008080:CDS 40.0%
TGTGTGATCTGCAATCAACT+GGG + chr7_1:8018529-8018548 Msa1008080:CDS 40.0%
GAAGCTTCTTACGTACTGTT+CGG + chr7_1:8018587-8018606 Msa1008080:CDS 40.0%
AAGCTTCTTACGTACTGTTC+GGG + chr7_1:8018588-8018607 Msa1008080:CDS 40.0%
AACATTCAGAATCAGATCCC+TGG + chr7_1:8018636-8018655 Msa1008080:CDS 40.0%
TCTGATACACAAAGTGACCA+GGG - chr7_1:8018656-8018675 None:intergenic 40.0%
CATCTAGATCAAGGTGAAGA+AGG + chr7_1:8018759-8018778 Msa1008080:CDS 40.0%
CAAGGTGAAGAAGGAGATAT+TGG + chr7_1:8018768-8018787 Msa1008080:CDS 40.0%
CAAACGACTTAAAAACTCCG+CGG - chr7_1:8018800-8018819 None:intergenic 40.0%
TAAGTCGTTTGCAACGTCAA+AGG + chr7_1:8018809-8018828 Msa1008080:CDS 40.0%
! GAGTATTTTGTGTGCCTCAA+TGG + chr7_1:8018978-8018997 Msa1008080:CDS 40.0%
AGAATCAGACAACGGCAATA+GGG - chr7_1:8019024-8019043 None:intergenic 40.0%
GATTTCTTTCTAGGTAGGGT+CGG + chr7_1:8019240-8019259 Msa1008080:intron 40.0%
!! CATGTCTTTTAGAGGGAATG+CGG - chr7_1:8019494-8019513 None:intergenic 40.0%
TGGTACAATCTCATCTTAGG+TGG - chr7_1:8019556-8019575 None:intergenic 40.0%
CTCAAGTAATGTCTTACTCG+AGG - chr7_1:8019726-8019745 None:intergenic 40.0%
TCGTGTAAGGATTGAGTCAA+TGG + chr7_1:8019829-8019848 Msa1008080:intron 40.0%
ATAGAGAACAGTAGAAGGTC+TGG - chr7_1:8019997-8020016 None:intergenic 40.0%
TAGCATGATCCATCTATTGG+AGG + chr7_1:8020349-8020368 Msa1008080:three_prime_UTR 40.0%
! AATGTCTGGTTGGTATGCTT+TGG + chr7_1:8020413-8020432 Msa1008080:three_prime_UTR 40.0%
CTTCAATTTCCACTCAGAGG+TGG + chr7_1:8016457-8016476 Msa1008080:five_prime_UTR 45.0%
TAGAAAAACCCACCTCTGAG+TGG - chr7_1:8016469-8016488 None:intergenic 45.0%
!! ACTCAGAGGTGGGTTTTTCT+AGG + chr7_1:8016468-8016487 Msa1008080:five_prime_UTR 45.0%
!! CTCAGAGGTGGGTTTTTCTA+GGG + chr7_1:8016469-8016488 Msa1008080:five_prime_UTR 45.0%
!!! TGGGTTTTTCTAGGGTTTCG+AGG + chr7_1:8016477-8016496 Msa1008080:five_prime_UTR 45.0%
GTGATCTATGATCTTGGGAC+TGG + chr7_1:8016645-8016664 Msa1008080:intron 45.0%
! GGTCATTGATTGCAGACGAA+AGG + chr7_1:8016915-8016934 Msa1008080:intron 45.0%
CGTTACACTGAACAACAAGC+TGG - chr7_1:8017063-8017082 None:intergenic 45.0%
GCTTGATGTGCAACGTCAAA+CGG - chr7_1:8017114-8017133 None:intergenic 45.0%
TTTGACGTTGCACATCAAGC+AGG + chr7_1:8017114-8017133 Msa1008080:five_prime_UTR 45.0%
TTGACGTTGCACATCAAGCA+GGG + chr7_1:8017115-8017134 Msa1008080:five_prime_UTR 45.0%
CAGCCCAAACCCATCATTAA+TGG - chr7_1:8017353-8017372 None:intergenic 45.0%
!! CATTAATGATGGGTTTGGGC+TGG + chr7_1:8017351-8017370 Msa1008080:five_prime_UTR 45.0%
AAGACTGTTTGTGGGGCTTA+TGG + chr7_1:8017409-8017428 Msa1008080:five_prime_UTR 45.0%
TTAACGATCCTGCAAGCGTA+TGG - chr7_1:8017476-8017495 None:intergenic 45.0%
!! GAACTGGTTTGTGAGTACCA+TGG + chr7_1:8018054-8018073 Msa1008080:five_prime_UTR 45.0%
GCTAGCTGTCAATGTTCCAA+AGG + chr7_1:8018351-8018370 Msa1008080:CDS 45.0%
GATAGAGCAGCAATCAGCTT+AGG - chr7_1:8018429-8018448 None:intergenic 45.0%
ATCTTGCCAAGCGAGTTCTA+TGG + chr7_1:8018472-8018491 Msa1008080:CDS 45.0%
CTCTTTCCATAGAACTCGCT+TGG - chr7_1:8018481-8018500 None:intergenic 45.0%
GTCTGATACACAAAGTGACC+AGG - chr7_1:8018657-8018676 None:intergenic 45.0%
CCTTGATCTAGATGCTCACA+AGG - chr7_1:8018753-8018772 None:intergenic 45.0%
CCTTGTGAGCATCTAGATCA+AGG + chr7_1:8018750-8018769 Msa1008080:CDS 45.0%
GGAGATATTGGATTCTTCCG+CGG + chr7_1:8018780-8018799 Msa1008080:CDS 45.0%
TGCCTCAATGGTCACTTGAT+TGG + chr7_1:8018990-8019009 Msa1008080:CDS 45.0%
GCCTCAATGGTCACTTGATT+GGG + chr7_1:8018991-8019010 Msa1008080:CDS 45.0%
TCCCAATCAAGTGACCATTG+AGG - chr7_1:8018995-8019014 None:intergenic 45.0%
CAGAATCAGACAACGGCAAT+AGG - chr7_1:8019025-8019044 None:intergenic 45.0%
GCCTCTTCAGAATCAGACAA+CGG - chr7_1:8019032-8019051 None:intergenic 45.0%
TCTAGGTAGGGTCGGTTATA+TGG + chr7_1:8019248-8019267 Msa1008080:intron 45.0%
GGAACCATGCATAGCTCAAT+AGG + chr7_1:8019408-8019427 Msa1008080:intron 45.0%
CACTCCTATTGAGCTATGCA+TGG - chr7_1:8019415-8019434 None:intergenic 45.0%
CATGCATAGCTCAATAGGAG+TGG + chr7_1:8019413-8019432 Msa1008080:intron 45.0%
TCTTCTGGTACCTCAGTAAC+AGG + chr7_1:8020075-8020094 Msa1008080:intron 45.0%
ACATCATAAACCACCCCAAG+AGG - chr7_1:8020228-8020247 None:intergenic 45.0%
TGATGTGCTATCGCGTATGT+TGG + chr7_1:8020242-8020261 Msa1008080:three_prime_UTR 45.0%
TAGATGCAGATCACTTGTGC+AGG + chr7_1:8020286-8020305 Msa1008080:three_prime_UTR 45.0%
!! GATCTTGGGACTGGTTGATG+TGG + chr7_1:8016654-8016673 Msa1008080:intron 50.0%
ATTGGGTTTCTAACTCCGGC+TGG + chr7_1:8017169-8017188 Msa1008080:five_prime_UTR 50.0%
TTGGGTTTCTAACTCCGGCT+GGG + chr7_1:8017170-8017189 Msa1008080:five_prime_UTR 50.0%
AACTCAACACTTCTCCCAGC+CGG - chr7_1:8017187-8017206 None:intergenic 50.0%
TGAAACCCACAACACACTGC+TGG + chr7_1:8018004-8018023 Msa1008080:five_prime_UTR 50.0%
TGCTTCCAGCAGTGTGTTGT+GGG - chr7_1:8018012-8018031 None:intergenic 50.0%
!! ATGGGAACTGGAGGGCATTA+GGG + chr7_1:8018565-8018584 Msa1008080:CDS 50.0%
TACTGTTCGGGATGCACGAA+AGG + chr7_1:8018600-8018619 Msa1008080:CDS 50.0%
! TTCCTCAAAGTGCTAGCTGC+AGG + chr7_1:8018931-8018950 Msa1008080:CDS 50.0%
AACCTGCAGCTAGCACTTTG+AGG - chr7_1:8018936-8018955 None:intergenic 50.0%
! TCAAAGTGCTAGCTGCAGGT+TGG + chr7_1:8018935-8018954 Msa1008080:CDS 50.0%
! CAAAGTGCTAGCTGCAGGTT+GGG + chr7_1:8018936-8018955 Msa1008080:CDS 50.0%
! GCCGTTGTCTGATTCTGAAG+AGG + chr7_1:8019028-8019047 Msa1008080:CDS 50.0%
!! TTCTGAAGAGGCGCCTGAAT+TGG + chr7_1:8019040-8019059 Msa1008080:CDS 50.0%
GAGCTATGCATGGTTCCATG+TGG - chr7_1:8019405-8019424 None:intergenic 50.0%
AAGGCAAATGGTGAGCCTCT+TGG + chr7_1:8020210-8020229 Msa1008080:three_prime_UTR 50.0%
AGGCAAATGGTGAGCCTCTT+GGG + chr7_1:8020211-8020230 Msa1008080:three_prime_UTR 50.0%
GTGCAACGTCAAACGGCATG+TGG - chr7_1:8017107-8017126 None:intergenic 55.0%
CTGCTTCCAGCAGTGTGTTG+TGG - chr7_1:8018013-8018032 None:intergenic 55.0%
TCCAGTTCCCATCAACGACG+TGG - chr7_1:8018557-8018576 None:intergenic 55.0%
! ACGTCGTTGATGGGAACTGG+AGG + chr7_1:8018556-8018575 Msa1008080:CDS 55.0%
! CGTCGTTGATGGGAACTGGA+GGG + chr7_1:8018557-8018576 Msa1008080:CDS 55.0%
!! GATGGGAACTGGAGGGCATT+AGG + chr7_1:8018564-8018583 Msa1008080:CDS 55.0%
TGTTCGGGATGCACGAAAGG+TGG + chr7_1:8018603-8018622 Msa1008080:CDS 55.0%
GGCAAATGGTGAGCCTCTTG+GGG + chr7_1:8020212-8020231 Msa1008080:three_prime_UTR 55.0%
AAATGGTGAGCCTCTTGGGG+TGG + chr7_1:8020215-8020234 Msa1008080:three_prime_UTR 55.0%
ACTGGGAGCCACGTCGTTGA+TGG + chr7_1:8018546-8018565 Msa1008080:CDS 60.0%
CTGGGAGCCACGTCGTTGAT+GGG + chr7_1:8018547-8018566 Msa1008080:CDS 60.0%
! GCCACGTCGTTGATGGGAAC+TGG + chr7_1:8018553-8018572 Msa1008080:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 8016324 8020696 8016324 ID=Msa1008080;Name=Msa1008080
chr7_1 mRNA 8016324 8020696 8016324 ID=Msa1008080-mRNA-1;Parent=Msa1008080;Name=Msa1008080-mRNA-1;_AED=0.03;_eAED=0.03;_QI=1467|1|1|1|0|0|3|546|250
chr7_1 exon 8016324 8016498 8016324 ID=Msa1008080-mRNA-1:exon:2423;Parent=Msa1008080-mRNA-1
chr7_1 exon 8017023 8019085 8017023 ID=Msa1008080-mRNA-1:exon:2424;Parent=Msa1008080-mRNA-1
chr7_1 exon 8020169 8020696 8020169 ID=Msa1008080-mRNA-1:exon:2425;Parent=Msa1008080-mRNA-1
chr7_1 five_prime_UTR 8016324 8016498 8016324 ID=Msa1008080-mRNA-1:five_prime_utr;Parent=Msa1008080-mRNA-1
chr7_1 five_prime_UTR 8017023 8018314 8017023 ID=Msa1008080-mRNA-1:five_prime_utr;Parent=Msa1008080-mRNA-1
chr7_1 CDS 8018315 8019067 8018315 ID=Msa1008080-mRNA-1:cds;Parent=Msa1008080-mRNA-1
chr7_1 three_prime_UTR 8019068 8019085 8019068 ID=Msa1008080-mRNA-1:three_prime_utr;Parent=Msa1008080-mRNA-1
chr7_1 three_prime_UTR 8020169 8020696 8020169 ID=Msa1008080-mRNA-1:three_prime_utr;Parent=Msa1008080-mRNA-1
Gene Sequence

>Msa1008080

ATGATTATCACAAAGCAGTACCGATGCATACATTCAGCTAGCTGTCAATGTTCCAAAGGGCATCTAAGTGAAGATGCGATTTTCTTAGTGTTTCATAATTTAAAATGGAATCCTAAGCTGATTGCTGCTCTATCATGTGTGTGCAAATGGTTTGACGATCTTGCCAAGCGAGTTCTATGGAAAGAGTTTTGTCGAACAAGAGCTCCAAAGATGTTGTGTGATCTGCAATCAACTGGGAGCCACGTCGTTGATGGGAACTGGAGGGCATTAGGGAAGCTTCTTACGTACTGTTCGGGATGCACGAAAGGTGGCTTGTTTAAAAACATTCAGAATCAGATCCCTGGTCACTTTGTGTATCAGACTCGATTTTCTAGAACATCAGGGAAGAGCTTTCTTTTGCCACAATGCAGAACTGATGTTTTGTATGTATCTGATCCTTGTGAGCATCTAGATCAAGGTGAAGAAGGAGATATTGGATTCTTCCGCGGAGTTTTTAAGTCGTTTGCAACGTCAAAGGTCAGAAAGATGCTTATTAGTAAAGGTGCTAAGCTTCATCAAACAGAAGTTTGCCCTTATTGTAAAGCGAAGTTGTGGAGCATGCAGCAAGCTAAAATGATTCCTCAAAGTGCTAGCTGCAGGTTGGGTTCTTATGAAGATGATATCGAGTATTTTGTGTGCCTCAATGGTCACTTGATTGGGATATGTACCCTATTGCCGTTGTCTGATTCTGAAGAGGCGCCTGAATTGGAGTAA
Protein sequence

>Msa1008080

MIITKQYRCIHSASCQCSKGHLSEDAIFLVFHNLKWNPKLIAALSCVCKWFDDLAKRVLWKEFCRTRAPKMLCDLQSTGSHVVDGNWRALGKLLTYCSGCTKGGLFKNIQNQIPGHFVYQTRFSRTSGKSFLLPQCRTDVLYVSDPCEHLDQGEEGDIGFFRGVFKSFATSKVRKMLISKGAKLHQTEVCPYCKAKLWSMQQAKMIPQSASCRLGSYEDDIEYFVCLNGHLIGICTLLPLSDSEEAPELE*