AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1045110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1045110 MtrunA17_Chr6g0454031 81.600 250 44 1 1 250 1 248 7.96e-159 440
Msa1045110 MtrunA17_Chr7g0218381 80.800 250 0 1 1 250 1 202 9.41e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1045110 AT5G39360.1 76.494 251 56 2 1 250 1 249 1.61e-148 414
Msa1045110 AT5G15440.1 76.423 246 57 1 1 246 1 245 9.89e-144 404
Msa1045110 AT3G63060.1 42.534 221 125 2 23 242 36 255 3.90e-63 199

Find 127 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAATATTCAACTAAA+AGG 0.164816 7_2:+4288404 None:intergenic
TAGTGTTTCATAATTTAAAA+TGG 0.209786 7_2:-4288089 Msa1045110:CDS
CACAACTTCGCTTTACAATA+AGG 0.238798 7_2:+4287604 None:intergenic
AACGATAATGCGACTTTCAT+TGG 0.271652 7_2:-4289339 Msa1045110:five_prime_UTR
CAAGGTGAAGAAGGAGATAT+TGG 0.288349 7_2:-4287721 Msa1045110:CDS
TTCAACTATTACTCCAATTC+AGG 0.290810 7_2:+4287436 None:intergenic
CAGCCCAAACCCATCATTAA+TGG 0.299946 7_2:+4289140 None:intergenic
GATGGGAACTGGAGGGCATT+AGG 0.301055 7_2:-4287925 Msa1045110:CDS
AGGCAAATGGTGAGCCTCTT+GGG 0.305235 7_2:-4286277 Msa1045110:three_prime_UTR
TGCAGATCACACAACATCTT+TGG 0.305769 7_2:+4287970 None:intergenic
CAGATTATTTACAAGTTGTT+TGG 0.310423 7_2:+4285886 None:intergenic
AAGACTGTTTGTGGGGCTTA+TGG 0.311944 7_2:-4289081 Msa1045110:five_prime_UTR
ACTTATTTCCATACGCTTGC+AGG 0.313651 7_2:-4289025 Msa1045110:five_prime_UTR
AGCTAATATGAGGGGCAATT+TGG 0.322687 7_2:+4290119 None:intergenic
TTGTGAATATTTGAACTCTT+AGG 0.332759 7_2:-4288835 Msa1045110:five_prime_UTR
CAGAATCAGACAACGGCAAT+AGG 0.334261 7_2:+4287467 None:intergenic
ACAATATGTTTGGTTTGGTT+TGG 0.340993 7_2:+4288591 None:intergenic
TTCTGAAGAGGCGCCTGAAT+TGG 0.352655 7_2:-4287449 Msa1045110:CDS
ACATCCATTAATGATGGGTT+TGG 0.356850 7_2:-4289144 Msa1045110:five_prime_UTR
CTCTATCATGTGTGTGCAAA+TGG 0.361648 7_2:-4288047 Msa1045110:CDS
CTACAAATTTACAATATGTT+TGG 0.363437 7_2:+4288581 None:intergenic
ACGATTATTTACATCATGTT+TGG 0.365570 7_2:+4285925 None:intergenic
TGCTTCCAGCAGTGTGTTGT+GGG 0.366169 7_2:+4288480 None:intergenic
CAAAGTGATTGAGTATTGTT+TGG 0.369583 7_2:-4288746 Msa1045110:five_prime_UTR
AATGTCTGGTTGGTATGCTT+TGG 0.390732 7_2:-4286075 Msa1045110:three_prime_UTR
ACAACTTCGCTTTACAATAA+GGG 0.398128 7_2:+4287605 None:intergenic
TGCCTCAATGGTCACTTGAT+TGG 0.401501 7_2:-4287499 Msa1045110:CDS
CAAAGTGCTAGCTGCAGGTT+GGG 0.401792 7_2:-4287553 Msa1045110:CDS
TGTATGTTGCTGCTAAAATT+AGG 0.405550 7_2:-4288782 Msa1045110:five_prime_UTR
AATACAACACCTCCAATAGA+TGG 0.406390 7_2:+4286130 None:intergenic
AATTTACAATATGTTTGGTT+TGG 0.410012 7_2:+4288586 None:intergenic
GCCACGTCGTTGATGGGAAC+TGG 0.411747 7_2:-4287936 Msa1045110:CDS
TTGTGTGATCTGCAATCAAC+TGG 0.417058 7_2:-4287961 Msa1045110:CDS
AAGCTTCTTACGTACTGTTC+GGG 0.422997 7_2:-4287901 Msa1045110:CDS
ACTGGGAGCCACGTCGTTGA+TGG 0.424116 7_2:-4287943 Msa1045110:CDS
CATCCATTAATGATGGGTTT+GGG 0.424733 7_2:-4289143 Msa1045110:five_prime_UTR
ATTGGGTTTCTAACTCCGGC+TGG 0.427801 7_2:-4289321 Msa1045110:five_prime_UTR
ATGGGAACTGGAGGGCATTA+GGG 0.429718 7_2:-4287924 Msa1045110:CDS
ATCTTCACTTAGATGCCCTT+TGG 0.429745 7_2:+4288122 None:intergenic
GAAGCTTCTTACGTACTGTT+CGG 0.432478 7_2:-4287902 Msa1045110:CDS
CTAAACACATCCATTAATGA+TGG 0.436220 7_2:-4289150 Msa1045110:five_prime_UTR
TTCTGTAATTATACTTGTGA+TGG 0.437144 7_2:-4288645 Msa1045110:five_prime_UTR
ACGATAATGCGACTTTCATT+GGG 0.438422 7_2:-4289338 Msa1045110:five_prime_UTR
AACCTGCAGCTAGCACTTTG+AGG 0.444997 7_2:+4287556 None:intergenic
TTGTACCGATAGCAATTCCT+TGG 0.446337 7_2:-4289195 Msa1045110:five_prime_UTR
TGATATCTTTCCTCTAATGA+AGG 0.451969 7_2:-4289226 Msa1045110:five_prime_UTR
CTGCTTCCAGCAGTGTGTTG+TGG 0.455287 7_2:+4288479 None:intergenic
GCCTCAATGGTCACTTGATT+GGG 0.456870 7_2:-4287498 Msa1045110:CDS
CATCTAGATCAAGGTGAAGA+AGG 0.463015 7_2:-4287730 Msa1045110:CDS
AAGGCAAATGGTGAGCCTCT+TGG 0.463223 7_2:-4286278 Msa1045110:three_prime_UTR
CTCTTTCCATAGAACTCGCT+TGG 0.463797 7_2:+4288011 None:intergenic
GCTTGATGTGCAACGTCAAA+CGG 0.468341 7_2:+4289379 None:intergenic
AAAACACCGTCCTTCATTAG+AGG 0.473973 7_2:+4289216 None:intergenic
ATCTTGCCAAGCGAGTTCTA+TGG 0.476286 7_2:-4288017 Msa1045110:CDS
AAATTAATATTCAACTAAAA+GGG 0.477316 7_2:+4288405 None:intergenic
CATTAATGATGGGTTTGGGC+TGG 0.477597 7_2:-4289139 Msa1045110:five_prime_UTR
ATGTAGCATGATCCATCTAT+TGG 0.479951 7_2:-4286142 Msa1045110:three_prime_UTR
CATCTTGAATTTAGCAGAGT+CGG 0.482579 7_2:+4286304 None:intergenic
AACTTGTGTAAGACTGTTTG+TGG 0.486725 7_2:-4289090 Msa1045110:five_prime_UTR
TCAAAGTGCTAGCTGCAGGT+TGG 0.489268 7_2:-4287554 Msa1045110:CDS
TGTACCGATAGCAATTCCTT+GGG 0.492322 7_2:-4289194 Msa1045110:five_prime_UTR
AGAATCAGACAACGGCAATA+GGG 0.497429 7_2:+4287468 None:intergenic
CAAACAATACTCAATCACTT+TGG 0.501113 7_2:+4288747 None:intergenic
TTCAAGATGAAGAAGGCAAA+TGG 0.503851 7_2:-4286290 Msa1045110:three_prime_UTR
TGAAACCCACAACACACTGC+TGG 0.504466 7_2:-4288485 Msa1045110:five_prime_UTR
CTGGGAGCCACGTCGTTGAT+GGG 0.504476 7_2:-4287942 Msa1045110:CDS
TTTCATTGGGTTTCTAACTC+CGG 0.505320 7_2:-4289325 Msa1045110:five_prime_UTR
AAATTGCCCCTCATATTAGC+TGG 0.506482 7_2:-4290117 Msa1045110:five_prime_UTR
GTGAAGAATACTCATAGTAT+AGG 0.509863 7_2:-4286180 Msa1045110:three_prime_UTR
TATGTAAGAACACTTATGCT+TGG 0.512763 7_2:-4285968 Msa1045110:three_prime_UTR
GATAGAGCAGCAATCAGCTT+AGG 0.512808 7_2:+4288063 None:intergenic
TAAACACATCCATTAATGAT+GGG 0.518097 7_2:-4289149 Msa1045110:five_prime_UTR
TTTGACGTTGCACATCAAGC+AGG 0.518359 7_2:-4289376 Msa1045110:five_prime_UTR
TTATTTACAAGTTGTTTGGA+TGG 0.527757 7_2:+4285890 None:intergenic
AGAAAGATGCTTATTAGTAA+AGG 0.529828 7_2:-4287655 Msa1045110:CDS
AACTTGTAAATAATCTGACT+TGG 0.531224 7_2:-4285880 Msa1045110:three_prime_UTR
TTATTTACATCATGTTTGGA+TGG 0.531853 7_2:+4285929 None:intergenic
CTTCAATTTCCACTCAGAGG+TGG 0.535038 7_2:-4290032 Msa1045110:five_prime_UTR
ACAAACTATACTGATTATGT+TGG 0.537557 7_2:-4285857 Msa1045110:three_prime_UTR
TTCCTCAAAGTGCTAGCTGC+AGG 0.540338 7_2:-4287558 Msa1045110:CDS
TTAACGATCCTGCAAGCGTA+TGG 0.541472 7_2:+4289017 None:intergenic
TTCAATTTCCACTCAGAGGT+GGG 0.542737 7_2:-4290031 Msa1045110:five_prime_UTR
AAATGGTGAGCCTCTTGGGG+TGG 0.542820 7_2:-4286273 Msa1045110:three_prime_UTR
TGCTAAATTCAAGATGAAGA+AGG 0.543882 7_2:-4286297 Msa1045110:three_prime_UTR
ACTAAAAGGGTTGCAATCCA+TGG 0.544566 7_2:+4288418 None:intergenic
ATTTGGTCAAGAAATGAGTA+AGG 0.545610 7_2:+4290136 None:intergenic
AACTCAACACTTCTCCCAGC+CGG 0.547403 7_2:+4289306 None:intergenic
GCCGTTGTCTGATTCTGAAG+AGG 0.548453 7_2:-4287461 Msa1045110:CDS
AACATTCAGAATCAGATCCC+TGG 0.551777 7_2:-4287853 Msa1045110:CDS
TTGGGTTTCTAACTCCGGCT+GGG 0.554045 7_2:-4289320 Msa1045110:five_prime_UTR
TAGATGCAGATCACTTGTGC+AGG 0.554067 7_2:-4286202 Msa1045110:three_prime_UTR
CCTTGTGAGCATCTAGATCA+AGG 0.554478 7_2:-4287739 Msa1045110:CDS
ACTTGTGTAAGACTGTTTGT+GGG 0.554723 7_2:-4289089 Msa1045110:five_prime_UTR
GTCTGATACACAAAGTGACC+AGG 0.560165 7_2:+4287835 None:intergenic
CTAGCTGTCAATGTTCCAAA+GGG 0.560254 7_2:-4288137 Msa1045110:CDS
CCTTGATCTAGATGCTCACA+AGG 0.564697 7_2:+4287739 None:intergenic
TGATGTGCTATCGCGTATGT+TGG 0.566092 7_2:-4286246 Msa1045110:three_prime_UTR
TTCTTGGTTAGCAAACATCA+TGG 0.570595 7_2:+4288244 None:intergenic
GCTAGCTGTCAATGTTCCAA+AGG 0.578026 7_2:-4288138 Msa1045110:CDS
TAAGTCGTTTGCAACGTCAA+AGG 0.578884 7_2:-4287680 Msa1045110:CDS
TTAAGCAGTTTATCTAAACA+TGG 0.585305 7_2:-4288881 Msa1045110:five_prime_UTR
CGTTACACTGAACAACAAGC+TGG 0.585680 7_2:+4289430 None:intergenic
TTGACGTTGCACATCAAGCA+GGG 0.587625 7_2:-4289375 Msa1045110:five_prime_UTR
TAGCATGATCCATCTATTGG+AGG 0.588497 7_2:-4286139 Msa1045110:three_prime_UTR
ATCTTTCCTCTAATGAAGGA+CGG 0.589704 7_2:-4289222 Msa1045110:five_prime_UTR
CTTATTGTAAAGCGAAGTTG+TGG 0.591906 7_2:-4287603 Msa1045110:CDS
ACGTCGTTGATGGGAACTGG+AGG 0.594238 7_2:-4287933 Msa1045110:CDS
TACTCATAGTATAGGTGACT+AGG 0.595516 7_2:-4286172 Msa1045110:three_prime_UTR
TATACCCAAGGAATTGCTAT+CGG 0.597827 7_2:+4289190 None:intergenic
TTTGTAAGCACGATATCATG+AGG 0.601873 7_2:+4285806 None:intergenic
TAACTTCAATTTCCACTCAG+AGG 0.610940 7_2:-4290035 Msa1045110:five_prime_UTR
AAAACATCAGTTCTGCATTG+TGG 0.613771 7_2:+4287775 None:intergenic
GGCAAATGGTGAGCCTCTTG+GGG 0.617357 7_2:-4286276 Msa1045110:three_prime_UTR
AGCTAGCTGAATGTATGCAT+CGG 0.619048 7_2:+4288154 None:intergenic
TGTTCGGGATGCACGAAAGG+TGG 0.621782 7_2:-4287886 Msa1045110:CDS
CTTGTGTAAGACTGTTTGTG+GGG 0.622230 7_2:-4289088 Msa1045110:five_prime_UTR
GGAGATATTGGATTCTTCCG+CGG 0.632203 7_2:-4287709 Msa1045110:CDS
ACATCATAAACCACCCCAAG+AGG 0.632395 7_2:+4286263 None:intergenic
CACATGAGGGAAATAAAACA+AGG 0.636730 7_2:-4288674 Msa1045110:five_prime_UTR
TCCAGTTCCCATCAACGACG+TGG 0.656887 7_2:+4287935 None:intergenic
CGTCGTTGATGGGAACTGGA+GGG 0.667520 7_2:-4287932 Msa1045110:CDS
TCCCAATCAAGTGACCATTG+AGG 0.669913 7_2:+4287497 None:intergenic
GCCTCTTCAGAATCAGACAA+CGG 0.682457 7_2:+4287460 None:intergenic
GAACTGGTTTGTGAGTACCA+TGG 0.688393 7_2:-4288435 Msa1045110:five_prime_UTR
TCTGATACACAAAGTGACCA+GGG 0.703260 7_2:+4287836 None:intergenic
TGTGTGATCTGCAATCAACT+GGG 0.707515 7_2:-4287960 Msa1045110:CDS
GTGCAACGTCAAACGGCATG+TGG 0.713160 7_2:+4289386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATATTCAACTAAAA+GGG + chr7_2:4287579-4287598 None:intergenic 10.0%
!! AAAATTAATATTCAACTAAA+AGG + chr7_2:4287580-4287599 None:intergenic 10.0%
!! TATCAGTTTATAAAAATTCT+TGG + chr7_2:4287756-4287775 None:intergenic 15.0%
!! TAGTGTTTCATAATTTAAAA+TGG - chr7_2:4287892-4287911 Msa1045110:CDS 15.0%
!!! TTTATTTTTTATTTCGTGTA+AGG - chr7_2:4289309-4289328 Msa1045110:five_prime_UTR 15.0%
!!! TGATTTTATTCGATTTGTAT+TGG - chr7_2:4286036-4286055 Msa1045110:three_prime_UTR 20.0%
!!! TTGATTTGCATATAATTGAT+GGG - chr7_2:4286104-4286123 Msa1045110:three_prime_UTR 20.0%
!!! AAAGATATTTTTGTAGAAGA+GGG - chr7_2:4286711-4286730 Msa1045110:intron 20.0%
!!! TAATATTTTAAGAGCTTGTT+TGG - chr7_2:4287269-4287288 Msa1045110:intron 20.0%
!!! AATATTTTAAGAGCTTGTTT+GGG - chr7_2:4287270-4287289 Msa1045110:intron 20.0%
!! AATTTACAATATGTTTGGTT+TGG + chr7_2:4287398-4287417 None:intergenic 20.0%
!! CTACAAATTTACAATATGTT+TGG + chr7_2:4287403-4287422 None:intergenic 20.0%
!!! TGTTTTGCTTAAGTATATAT+TGG - chr7_2:4287599-4287618 Msa1045110:CDS 20.0%
!!! ATTTTTCTTTTAGTTTTGCT+AGG - chr7_2:4287776-4287795 Msa1045110:CDS 20.0%
!! AACAGAAAAATAGTAAGTAA+TGG + chr7_2:4288603-4288622 None:intergenic 20.0%
!! ATAATCTATGATTTCTTTCT+AGG - chr7_2:4288723-4288742 Msa1045110:five_prime_UTR 20.0%
!! TTTAGACCTAATAATTGATA+GGG + chr7_2:4288782-4288801 None:intergenic 20.0%
!! AAAAGATAGTTAGAAGTAAA+TGG + chr7_2:4288811-4288830 None:intergenic 20.0%
!!! TTTAAAACATGTCTTTTAGA+GGG + chr7_2:4288994-4289013 None:intergenic 20.0%
!!! ATTTAAAACATGTCTTTTAG+AGG + chr7_2:4288995-4289014 None:intergenic 20.0%
!!! ATTTGCTAGTTATATATGTA+AGG - chr7_2:4289508-4289527 Msa1045110:intron 20.0%
!!! ATGTGATTAAATTAGTTTGA+AGG - chr7_2:4289606-4289625 Msa1045110:intron 20.0%
!!! CTTGATTTGCATATAATTGA+TGG - chr7_2:4286103-4286122 Msa1045110:three_prime_UTR 25.0%
! TATAATTGATGGGAAATTTC+TGG - chr7_2:4286114-4286133 Msa1045110:three_prime_UTR 25.0%
! ATAATTGATGGGAAATTTCT+GGG - chr7_2:4286115-4286134 Msa1045110:three_prime_UTR 25.0%
!!! CAAAGATATTTTTGTAGAAG+AGG - chr7_2:4286710-4286729 Msa1045110:intron 25.0%
! TGATTACAAAAATATACCCA+AGG + chr7_2:4286806-4286825 None:intergenic 25.0%
! TAAACACATCCATTAATGAT+GGG - chr7_2:4286832-4286851 Msa1045110:intron 25.0%
! TTAAGCAGTTTATCTAAACA+TGG - chr7_2:4287100-4287119 Msa1045110:intron 25.0%
! TTGTGAATATTTGAACTCTT+AGG - chr7_2:4287146-4287165 Msa1045110:intron 25.0%
!!! ATATTTTAAGAGCTTGTTTG+GGG - chr7_2:4287271-4287290 Msa1045110:intron 25.0%
!! ACAAAATTTTGAACACATGA+GGG - chr7_2:4287294-4287313 Msa1045110:intron 25.0%
! TTCTGTAATTATACTTGTGA+TGG - chr7_2:4287336-4287355 Msa1045110:intron 25.0%
!! GTAGTCTTCATTTTCATTTT+TGG - chr7_2:4287419-4287438 Msa1045110:intron 25.0%
!!! TCATTTTTGTTTTCTTGAAC+TGG - chr7_2:4287530-4287549 Msa1045110:CDS 25.0%
! AGAAAGATGCTTATTAGTAA+AGG - chr7_2:4288326-4288345 Msa1045110:five_prime_UTR 25.0%
!! GTAATAGTTGAATGATTTTC+AGG - chr7_2:4288556-4288575 Msa1045110:five_prime_UTR 25.0%
! GTAAATCCCTATCAATTATT+AGG - chr7_2:4288773-4288792 Msa1045110:five_prime_UTR 25.0%
! CTTTAGACCTAATAATTGAT+AGG + chr7_2:4288783-4288802 None:intergenic 25.0%
!! CTAACTATCTTTTTCTGATT+TGG - chr7_2:4288818-4288837 Msa1045110:five_prime_UTR 25.0%
!! AAAATCAAAGACAAGAGTAT+AGG + chr7_2:4288865-4288884 None:intergenic 25.0%
! AATCATTAGAGAGAAAAAGA+TGG + chr7_2:4289069-4289088 None:intergenic 25.0%
! AAATTGTATGTTGAATTCAG+TGG - chr7_2:4289240-4289259 Msa1045110:five_prime_UTR 25.0%
!! CTCATTAGATATTTTGCAAA+TGG - chr7_2:4289356-4289375 Msa1045110:five_prime_UTR 25.0%
! AATTACTATAATTGCACTAG+TGG - chr7_2:4289391-4289410 Msa1045110:five_prime_UTR 25.0%
! AGGATGTATCTGATTATATT+TGG - chr7_2:4289528-4289547 Msa1045110:intron 25.0%
!! ATTCTATCATTTTCAATGTC+TGG - chr7_2:4289892-4289911 Msa1045110:intron 25.0%
! TTATTTACATCATGTTTGGA+TGG + chr7_2:4290055-4290074 None:intergenic 25.0%
! ACGATTATTTACATCATGTT+TGG + chr7_2:4290059-4290078 None:intergenic 25.0%
! TTATTTACAAGTTGTTTGGA+TGG + chr7_2:4290094-4290113 None:intergenic 25.0%
! CAGATTATTTACAAGTTGTT+TGG + chr7_2:4290098-4290117 None:intergenic 25.0%
! AACTTGTAAATAATCTGACT+TGG - chr7_2:4290101-4290120 Msa1045110:five_prime_UTR 25.0%
!! ACAAACTATACTGATTATGT+TGG - chr7_2:4290124-4290143 Msa1045110:five_prime_UTR 25.0%
ATTTGGTCAAGAAATGAGTA+AGG + chr7_2:4285848-4285867 None:intergenic 30.0%
! AATTTTCCAGCTAATATGAG+GGG + chr7_2:4285873-4285892 None:intergenic 30.0%
! CAATTTTCCAGCTAATATGA+GGG + chr7_2:4285874-4285893 None:intergenic 30.0%
TTATAGCTAGCTGATAACTT+TGG - chr7_2:4286264-4286283 Msa1045110:three_prime_UTR 30.0%
ATCAGCAGTCAATTAATTGA+TGG - chr7_2:4286311-4286330 Msa1045110:three_prime_UTR 30.0%
TCAGCAGTCAATTAATTGAT+GGG - chr7_2:4286312-4286331 Msa1045110:three_prime_UTR 30.0%
TGATATCTTTCCTCTAATGA+AGG - chr7_2:4286755-4286774 Msa1045110:intron 30.0%
CTAAACACATCCATTAATGA+TGG - chr7_2:4286831-4286850 Msa1045110:intron 30.0%
! TGGCTTTTATGTATGATTTG+AGG - chr7_2:4287120-4287139 Msa1045110:intron 30.0%
! GGCTTTTATGTATGATTTGA+GGG - chr7_2:4287121-4287140 Msa1045110:intron 30.0%
TGTATGTTGCTGCTAAAATT+AGG - chr7_2:4287199-4287218 Msa1045110:intron 30.0%
CAAACAATACTCAATCACTT+TGG + chr7_2:4287237-4287256 None:intergenic 30.0%
! CAAAGTGATTGAGTATTGTT+TGG - chr7_2:4287235-4287254 Msa1045110:intron 30.0%
! GACAAAATTTTGAACACATG+AGG - chr7_2:4287293-4287312 Msa1045110:intron 30.0%
!! ACAATATGTTTGGTTTGGTT+TGG + chr7_2:4287393-4287412 None:intergenic 30.0%
!! GTGTTTGAGTTTTGTCTATT+TGG - chr7_2:4287708-4287727 Msa1045110:CDS 30.0%
ACAACTTCGCTTTACAATAA+GGG + chr7_2:4288379-4288398 None:intergenic 30.0%
TTCAACTATTACTCCAATTC+AGG + chr7_2:4288548-4288567 None:intergenic 30.0%
!! TTCAGGTATTTTTGTGTATC+TGG - chr7_2:4288573-4288592 Msa1045110:five_prime_UTR 30.0%
!! CTTTACTTTTCTTTGAGAAC+TGG - chr7_2:4288626-4288645 Msa1045110:five_prime_UTR 30.0%
TCTATGATTTCTTTCTAGGT+AGG - chr7_2:4288727-4288746 Msa1045110:five_prime_UTR 30.0%
CTATGATTTCTTTCTAGGTA+GGG - chr7_2:4288728-4288747 Msa1045110:five_prime_UTR 30.0%
GGAAGACTAAGGAAAAATAT+GGG + chr7_2:4288844-4288863 None:intergenic 30.0%
AGGAAGACTAAGGAAAAATA+TGG + chr7_2:4288845-4288864 None:intergenic 30.0%
!! TCATAGTTTTCTGTTGCTTA+GGG + chr7_2:4288936-4288955 None:intergenic 30.0%
!! TTCATAGTTTTCTGTTGCTT+AGG + chr7_2:4288937-4288956 None:intergenic 30.0%
AATGCTACACAAGATAAGAT+AGG + chr7_2:4289103-4289122 None:intergenic 30.0%
AATATTAGCATGTGATACAC+AGG + chr7_2:4289288-4289307 None:intergenic 30.0%
TGTTCAAAACAGTTTGACAA+TGG + chr7_2:4289437-4289456 None:intergenic 30.0%
AATGATTAGCAACTATCTTC+TGG - chr7_2:4289553-4289572 Msa1045110:intron 30.0%
ATACAATTAACCTGTTACTG+AGG + chr7_2:4289581-4289600 None:intergenic 30.0%
TGCTAAATTCAAGATGAAGA+AGG - chr7_2:4289684-4289703 Msa1045110:intron 30.0%
GTGAAGAATACTCATAGTAT+AGG - chr7_2:4289801-4289820 Msa1045110:intron 30.0%
! TATCATTTTCAATGTCTGGT+TGG - chr7_2:4289896-4289915 Msa1045110:intron 30.0%
TATGTAAGAACACTTATGCT+TGG - chr7_2:4290013-4290032 Msa1045110:five_prime_UTR 30.0%
! GCAATTTTCCAGCTAATATG+AGG + chr7_2:4285875-4285894 None:intergenic 35.0%
TAACTTCAATTTCCACTCAG+AGG - chr7_2:4285946-4285965 Msa1045110:three_prime_UTR 35.0%
GTTACTGTCATTAAATGTCG+CGG - chr7_2:4286386-4286405 Msa1045110:intron 35.0%
AGCTACAACAGAGAAAAATG+TGG + chr7_2:4286496-4286515 None:intergenic 35.0%
AACGATAATGCGACTTTCAT+TGG - chr7_2:4286642-4286661 Msa1045110:intron 35.0%
ACGATAATGCGACTTTCATT+GGG - chr7_2:4286643-4286662 Msa1045110:intron 35.0%
TTTCATTGGGTTTCTAACTC+CGG - chr7_2:4286656-4286675 Msa1045110:intron 35.0%
ATCTTTCCTCTAATGAAGGA+CGG - chr7_2:4286759-4286778 Msa1045110:intron 35.0%
TATACCCAAGGAATTGCTAT+CGG + chr7_2:4286794-4286813 None:intergenic 35.0%
!! ACATCCATTAATGATGGGTT+TGG - chr7_2:4286837-4286856 Msa1045110:intron 35.0%
!! CATCCATTAATGATGGGTTT+GGG - chr7_2:4286838-4286857 Msa1045110:intron 35.0%
AACTTGTGTAAGACTGTTTG+TGG - chr7_2:4286891-4286910 Msa1045110:intron 35.0%
ACTTGTGTAAGACTGTTTGT+GGG - chr7_2:4286892-4286911 Msa1045110:intron 35.0%
CACATGAGGGAAATAAAACA+AGG - chr7_2:4287307-4287326 Msa1045110:intron 35.0%
TTCTTGGTTAGCAAACATCA+TGG + chr7_2:4287740-4287759 None:intergenic 35.0%
!!! ACTCGATTTTCTAGAACATC+AGG - chr7_2:4288167-4288186 Msa1045110:CDS 35.0%
!!! CTCGATTTTCTAGAACATCA+GGG - chr7_2:4288168-4288187 Msa1045110:CDS 35.0%
AAAACATCAGTTCTGCATTG+TGG + chr7_2:4288209-4288228 None:intergenic 35.0%
CACAACTTCGCTTTACAATA+AGG + chr7_2:4288380-4288399 None:intergenic 35.0%
CTTATTGTAAAGCGAAGTTG+TGG - chr7_2:4288378-4288397 Msa1045110:five_prime_UTR 35.0%
! GATCCATTTTGAATGTTAGC+AGG - chr7_2:4288648-4288667 Msa1045110:five_prime_UTR 35.0%
CTACCTGCTAACATTCAAAA+TGG + chr7_2:4288654-4288673 None:intergenic 35.0%
! ACAAGAGTATAGGAAGACTA+AGG + chr7_2:4288855-4288874 None:intergenic 35.0%
! GATTTTCTGACTTTTCCACA+TGG - chr7_2:4288879-4288898 Msa1045110:five_prime_UTR 35.0%
!! ATAGTTTTCTGTTGCTTAGG+GGG + chr7_2:4288934-4288953 None:intergenic 35.0%
!! CATAGTTTTCTGTTGCTTAG+GGG + chr7_2:4288935-4288954 None:intergenic 35.0%
CATCAAGCGAAAAAAAGTTG+AGG + chr7_2:4289020-4289039 None:intergenic 35.0%
AGATGGTACAATCTCATCTT+AGG + chr7_2:4289052-4289071 None:intergenic 35.0%
CATGTGATACACAGGAAAAA+AGG + chr7_2:4289280-4289299 None:intergenic 35.0%
ATTGAGTCAATGGTTAGTGA+TGG - chr7_2:4289332-4289351 Msa1045110:five_prime_UTR 35.0%
AGCAAATAGAGAACAGTAGA+AGG + chr7_2:4289495-4289514 None:intergenic 35.0%
CATCTTGAATTTAGCAGAGT+CGG + chr7_2:4289680-4289699 None:intergenic 35.0%
TTCAAGATGAAGAAGGCAAA+TGG - chr7_2:4289691-4289710 Msa1045110:intron 35.0%
TATCGCGTATGTTGGAAAAA+TGG - chr7_2:4289743-4289762 Msa1045110:intron 35.0%
TACTCATAGTATAGGTGACT+AGG - chr7_2:4289809-4289828 Msa1045110:intron 35.0%
ATGTAGCATGATCCATCTAT+TGG - chr7_2:4289839-4289858 Msa1045110:intron 35.0%
AATACAACACCTCCAATAGA+TGG + chr7_2:4289854-4289873 None:intergenic 35.0%
AGCTAATATGAGGGGCAATT+TGG + chr7_2:4285865-4285884 None:intergenic 40.0%
AAATTGCCCCTCATATTAGC+TGG - chr7_2:4285864-4285883 Msa1045110:three_prime_UTR 40.0%
TTCAATTTCCACTCAGAGGT+GGG - chr7_2:4285950-4285969 Msa1045110:three_prime_UTR 40.0%
TTCTGGGTGATCTATGATCT+TGG - chr7_2:4286131-4286150 Msa1045110:three_prime_UTR 40.0%
TCTGGGTGATCTATGATCTT+GGG - chr7_2:4286132-4286151 Msa1045110:three_prime_UTR 40.0%
AAAACACCGTCCTTCATTAG+AGG + chr7_2:4286768-4286787 None:intergenic 40.0%
! TTGTACCGATAGCAATTCCT+TGG - chr7_2:4286786-4286805 Msa1045110:intron 40.0%
! TGTACCGATAGCAATTCCTT+GGG - chr7_2:4286787-4286806 Msa1045110:intron 40.0%
CTTGTGTAAGACTGTTTGTG+GGG - chr7_2:4286893-4286912 Msa1045110:intron 40.0%
ACTTATTTCCATACGCTTGC+AGG - chr7_2:4286956-4286975 Msa1045110:intron 40.0%
ACTAAAAGGGTTGCAATCCA+TGG + chr7_2:4287566-4287585 None:intergenic 40.0%
AGCTAGCTGAATGTATGCAT+CGG + chr7_2:4287830-4287849 None:intergenic 40.0%
CTAGCTGTCAATGTTCCAAA+GGG - chr7_2:4287844-4287863 Msa1045110:CDS 40.0%
ATCTTCACTTAGATGCCCTT+TGG + chr7_2:4287862-4287881 None:intergenic 40.0%
CTCTATCATGTGTGTGCAAA+TGG - chr7_2:4287934-4287953 Msa1045110:CDS 40.0%
TGCAGATCACACAACATCTT+TGG + chr7_2:4288014-4288033 None:intergenic 40.0%
TTGTGTGATCTGCAATCAAC+TGG - chr7_2:4288020-4288039 Msa1045110:CDS 40.0%
TGTGTGATCTGCAATCAACT+GGG - chr7_2:4288021-4288040 Msa1045110:CDS 40.0%
GAAGCTTCTTACGTACTGTT+CGG - chr7_2:4288079-4288098 Msa1045110:CDS 40.0%
AAGCTTCTTACGTACTGTTC+GGG - chr7_2:4288080-4288099 Msa1045110:CDS 40.0%
AACATTCAGAATCAGATCCC+TGG - chr7_2:4288128-4288147 Msa1045110:CDS 40.0%
TCTGATACACAAAGTGACCA+GGG + chr7_2:4288148-4288167 None:intergenic 40.0%
CATCTAGATCAAGGTGAAGA+AGG - chr7_2:4288251-4288270 Msa1045110:five_prime_UTR 40.0%
CAAGGTGAAGAAGGAGATAT+TGG - chr7_2:4288260-4288279 Msa1045110:five_prime_UTR 40.0%
CAAACGACTTAAAAACTCCG+CGG + chr7_2:4288292-4288311 None:intergenic 40.0%
TAAGTCGTTTGCAACGTCAA+AGG - chr7_2:4288301-4288320 Msa1045110:five_prime_UTR 40.0%
! GAGTATTTTGTGTGCCTCAA+TGG - chr7_2:4288470-4288489 Msa1045110:five_prime_UTR 40.0%
AGAATCAGACAACGGCAATA+GGG + chr7_2:4288516-4288535 None:intergenic 40.0%
GATTTCTTTCTAGGTAGGGT+CGG - chr7_2:4288732-4288751 Msa1045110:five_prime_UTR 40.0%
!! CATGTCTTTTAGAGGGAATG+CGG + chr7_2:4288987-4289006 None:intergenic 40.0%
TGGTACAATCTCATCTTAGG+TGG + chr7_2:4289049-4289068 None:intergenic 40.0%
CTCAAGTAATGTCTTACTCG+AGG + chr7_2:4289219-4289238 None:intergenic 40.0%
TCGTGTAAGGATTGAGTCAA+TGG - chr7_2:4289322-4289341 Msa1045110:five_prime_UTR 40.0%
ATAGAGAACAGTAGAAGGTC+TGG + chr7_2:4289490-4289509 None:intergenic 40.0%
TAGCATGATCCATCTATTGG+AGG - chr7_2:4289842-4289861 Msa1045110:intron 40.0%
! AATGTCTGGTTGGTATGCTT+TGG - chr7_2:4289906-4289925 Msa1045110:intron 40.0%
CTTCAATTTCCACTCAGAGG+TGG - chr7_2:4285949-4285968 Msa1045110:three_prime_UTR 45.0%
TAGAAAAACCCACCTCTGAG+TGG + chr7_2:4285961-4285980 None:intergenic 45.0%
!! ACTCAGAGGTGGGTTTTTCT+AGG - chr7_2:4285960-4285979 Msa1045110:three_prime_UTR 45.0%
!! CTCAGAGGTGGGTTTTTCTA+GGG - chr7_2:4285961-4285980 Msa1045110:three_prime_UTR 45.0%
!!! TGGGTTTTTCTAGGGTTTCG+AGG - chr7_2:4285969-4285988 Msa1045110:three_prime_UTR 45.0%
GTGATCTATGATCTTGGGAC+TGG - chr7_2:4286137-4286156 Msa1045110:three_prime_UTR 45.0%
! GGTCATTGATTGCAGACGAA+AGG - chr7_2:4286407-4286426 Msa1045110:intron 45.0%
CGTTACACTGAACAACAAGC+TGG + chr7_2:4286554-4286573 None:intergenic 45.0%
GCTTGATGTGCAACGTCAAA+CGG + chr7_2:4286605-4286624 None:intergenic 45.0%
TTTGACGTTGCACATCAAGC+AGG - chr7_2:4286605-4286624 Msa1045110:intron 45.0%
TTGACGTTGCACATCAAGCA+GGG - chr7_2:4286606-4286625 Msa1045110:intron 45.0%
CAGCCCAAACCCATCATTAA+TGG + chr7_2:4286844-4286863 None:intergenic 45.0%
!! CATTAATGATGGGTTTGGGC+TGG - chr7_2:4286842-4286861 Msa1045110:intron 45.0%
AAGACTGTTTGTGGGGCTTA+TGG - chr7_2:4286900-4286919 Msa1045110:intron 45.0%
TTAACGATCCTGCAAGCGTA+TGG + chr7_2:4286967-4286986 None:intergenic 45.0%
!! GAACTGGTTTGTGAGTACCA+TGG - chr7_2:4287546-4287565 Msa1045110:CDS 45.0%
GCTAGCTGTCAATGTTCCAA+AGG - chr7_2:4287843-4287862 Msa1045110:CDS 45.0%
GATAGAGCAGCAATCAGCTT+AGG + chr7_2:4287921-4287940 None:intergenic 45.0%
ATCTTGCCAAGCGAGTTCTA+TGG - chr7_2:4287964-4287983 Msa1045110:CDS 45.0%
CTCTTTCCATAGAACTCGCT+TGG + chr7_2:4287973-4287992 None:intergenic 45.0%
GTCTGATACACAAAGTGACC+AGG + chr7_2:4288149-4288168 None:intergenic 45.0%
CCTTGATCTAGATGCTCACA+AGG + chr7_2:4288245-4288264 None:intergenic 45.0%
CCTTGTGAGCATCTAGATCA+AGG - chr7_2:4288242-4288261 Msa1045110:five_prime_UTR 45.0%
GGAGATATTGGATTCTTCCG+CGG - chr7_2:4288272-4288291 Msa1045110:five_prime_UTR 45.0%
TGCCTCAATGGTCACTTGAT+TGG - chr7_2:4288482-4288501 Msa1045110:five_prime_UTR 45.0%
GCCTCAATGGTCACTTGATT+GGG - chr7_2:4288483-4288502 Msa1045110:five_prime_UTR 45.0%
TCCCAATCAAGTGACCATTG+AGG + chr7_2:4288487-4288506 None:intergenic 45.0%
CAGAATCAGACAACGGCAAT+AGG + chr7_2:4288517-4288536 None:intergenic 45.0%
GCCTCTTCAGAATCAGACAA+CGG + chr7_2:4288524-4288543 None:intergenic 45.0%
TCTAGGTAGGGTCGGTTATA+TGG - chr7_2:4288740-4288759 Msa1045110:five_prime_UTR 45.0%
GGAACCATGCATAGCTCAAT+AGG - chr7_2:4288900-4288919 Msa1045110:five_prime_UTR 45.0%
CACTCCTATTGAGCTATGCA+TGG + chr7_2:4288907-4288926 None:intergenic 45.0%
CATGCATAGCTCAATAGGAG+TGG - chr7_2:4288905-4288924 Msa1045110:five_prime_UTR 45.0%
TCTTCTGGTACCTCAGTAAC+AGG - chr7_2:4289568-4289587 Msa1045110:intron 45.0%
ACATCATAAACCACCCCAAG+AGG + chr7_2:4289721-4289740 None:intergenic 45.0%
TGATGTGCTATCGCGTATGT+TGG - chr7_2:4289735-4289754 Msa1045110:intron 45.0%
TAGATGCAGATCACTTGTGC+AGG - chr7_2:4289779-4289798 Msa1045110:intron 45.0%
!! GATCTTGGGACTGGTTGATG+TGG - chr7_2:4286146-4286165 Msa1045110:three_prime_UTR 50.0%
ATTGGGTTTCTAACTCCGGC+TGG - chr7_2:4286660-4286679 Msa1045110:intron 50.0%
TTGGGTTTCTAACTCCGGCT+GGG - chr7_2:4286661-4286680 Msa1045110:intron 50.0%
AACTCAACACTTCTCCCAGC+CGG + chr7_2:4286678-4286697 None:intergenic 50.0%
TGAAACCCACAACACACTGC+TGG - chr7_2:4287496-4287515 Msa1045110:CDS 50.0%
TGCTTCCAGCAGTGTGTTGT+GGG + chr7_2:4287504-4287523 None:intergenic 50.0%
!! ATGGGAACTGGAGGGCATTA+GGG - chr7_2:4288057-4288076 Msa1045110:CDS 50.0%
TACTGTTCGGGATGCACGAA+AGG - chr7_2:4288092-4288111 Msa1045110:CDS 50.0%
! TTCCTCAAAGTGCTAGCTGC+AGG - chr7_2:4288423-4288442 Msa1045110:five_prime_UTR 50.0%
AACCTGCAGCTAGCACTTTG+AGG + chr7_2:4288428-4288447 None:intergenic 50.0%
! TCAAAGTGCTAGCTGCAGGT+TGG - chr7_2:4288427-4288446 Msa1045110:five_prime_UTR 50.0%
! CAAAGTGCTAGCTGCAGGTT+GGG - chr7_2:4288428-4288447 Msa1045110:five_prime_UTR 50.0%
! GCCGTTGTCTGATTCTGAAG+AGG - chr7_2:4288520-4288539 Msa1045110:five_prime_UTR 50.0%
!! TTCTGAAGAGGCGCCTGAAT+TGG - chr7_2:4288532-4288551 Msa1045110:five_prime_UTR 50.0%
GAGCTATGCATGGTTCCATG+TGG + chr7_2:4288897-4288916 None:intergenic 50.0%
AAGGCAAATGGTGAGCCTCT+TGG - chr7_2:4289703-4289722 Msa1045110:intron 50.0%
AGGCAAATGGTGAGCCTCTT+GGG - chr7_2:4289704-4289723 Msa1045110:intron 50.0%
GTGCAACGTCAAACGGCATG+TGG + chr7_2:4286598-4286617 None:intergenic 55.0%
CTGCTTCCAGCAGTGTGTTG+TGG + chr7_2:4287505-4287524 None:intergenic 55.0%
TCCAGTTCCCATCAACGACG+TGG + chr7_2:4288049-4288068 None:intergenic 55.0%
! ACGTCGTTGATGGGAACTGG+AGG - chr7_2:4288048-4288067 Msa1045110:CDS 55.0%
! CGTCGTTGATGGGAACTGGA+GGG - chr7_2:4288049-4288068 Msa1045110:CDS 55.0%
!! GATGGGAACTGGAGGGCATT+AGG - chr7_2:4288056-4288075 Msa1045110:CDS 55.0%
TGTTCGGGATGCACGAAAGG+TGG - chr7_2:4288095-4288114 Msa1045110:CDS 55.0%
GGCAAATGGTGAGCCTCTTG+GGG - chr7_2:4289705-4289724 Msa1045110:intron 55.0%
AAATGGTGAGCCTCTTGGGG+TGG - chr7_2:4289708-4289727 Msa1045110:intron 55.0%
ACTGGGAGCCACGTCGTTGA+TGG - chr7_2:4288038-4288057 Msa1045110:CDS 60.0%
CTGGGAGCCACGTCGTTGAT+GGG - chr7_2:4288039-4288058 Msa1045110:CDS 60.0%
! GCCACGTCGTTGATGGGAAC+TGG - chr7_2:4288045-4288064 Msa1045110:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 4285814 4290189 4285814 ID=Msa1045110;Name=Msa1045110
chr7_2 mRNA 4285814 4290189 4285814 ID=Msa1045110-mRNA-1;Parent=Msa1045110;Name=Msa1045110-mRNA-1;_AED=0.03;_eAED=0.03;_QI=1470|1|1|1|0|0|3|546|250
chr7_2 exon 4285814 4286341 4285814 ID=Msa1045110-mRNA-1:exon:1026;Parent=Msa1045110-mRNA-1
chr7_2 exon 4287426 4289489 4287426 ID=Msa1045110-mRNA-1:exon:1025;Parent=Msa1045110-mRNA-1
chr7_2 exon 4290013 4290189 4290013 ID=Msa1045110-mRNA-1:exon:1024;Parent=Msa1045110-mRNA-1
chr7_2 five_prime_UTR 4290013 4290189 4290013 ID=Msa1045110-mRNA-1:five_prime_utr;Parent=Msa1045110-mRNA-1
chr7_2 five_prime_UTR 4288197 4289489 4288197 ID=Msa1045110-mRNA-1:five_prime_utr;Parent=Msa1045110-mRNA-1
chr7_2 CDS 4287444 4288196 4287444 ID=Msa1045110-mRNA-1:cds;Parent=Msa1045110-mRNA-1
chr7_2 three_prime_UTR 4287426 4287443 4287426 ID=Msa1045110-mRNA-1:three_prime_utr;Parent=Msa1045110-mRNA-1
chr7_2 three_prime_UTR 4285814 4286341 4285814 ID=Msa1045110-mRNA-1:three_prime_utr;Parent=Msa1045110-mRNA-1
Gene Sequence

>Msa1045110

ATGATTATCACAAAGCAGTACCGATGCATACATTCAGCTAGCTGTCAATGTTCCAAAGGGCATCTAAGTGAAGATGCGATTTTCTTAGTGTTTCATAATTTAAAATGGAATCCTAAGCTGATTGCTGCTCTATCATGTGTGTGCAAATGGTTTGACGATCTTGCCAAGCGAGTTCTATGGAAAGAGTTTTGTCGAACAAGAGCTCCAAAGATGTTGTGTGATCTGCAATCAACTGGGAGCCACGTCGTTGATGGGAACTGGAGGGCATTAGGGAAGCTTCTTACGTACTGTTCGGGATGCACGAAAGGTGGCTTGTTTAAAAACATTCAGAATCAGATCCCTGGTCACTTTGTGTATCAGACTCGATTTTCTAGAACATCAGGGAAGAGCTTTCTTTTGCCACAATGCAGAACTGATGTTTTGTATGTATCTGATCCTTGTGAGCATCTAGATCAAGGTGAAGAAGGAGATATTGGATTCTTCCGCGGAGTTTTTAAGTCGTTTGCAACGTCAAAGGTCAGAAAGATGCTTATTAGTAAAGGTGCTAAGCTTCATCAAACAGAAGTTTGCCCTTATTGTAAAGCGAAGTTGTGGAGCATGCAGCAAGCTAAAATGATTCCTCAAAGTGCTAGCTGCAGGTTGGGTTCTTATGAAGATGATATCGAGTATTTTGTGTGCCTCAATGGTCACTTGATTGGGATATGTACCCTATTGCCGTTGTCTGATTCTGAAGAGGCGCCTGAATTGGAGTAA
Protein sequence

>Msa1045110

MIITKQYRCIHSASCQCSKGHLSEDAIFLVFHNLKWNPKLIAALSCVCKWFDDLAKRVLWKEFCRTRAPKMLCDLQSTGSHVVDGNWRALGKLLTYCSGCTKGGLFKNIQNQIPGHFVYQTRFSRTSGKSFLLPQCRTDVLYVSDPCEHLDQGEEGDIGFFRGVFKSFATSKVRKMLISKGAKLHQTEVCPYCKAKLWSMQQAKMIPQSASCRLGSYEDDIEYFVCLNGHLIGICTLLPLSDSEEAPELE*