AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1137280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1137280 MtrunA17_Chr6g0454031 81.600 250 44 1 1 250 1 248 7.96e-159 440
Msa1137280 MtrunA17_Chr7g0218381 80.800 250 0 1 1 250 1 202 9.41e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1137280 AT5G39360.1 76.494 251 56 2 1 250 1 249 1.61e-148 414
Msa1137280 AT5G15440.1 76.423 246 57 1 1 246 1 245 9.89e-144 404
Msa1137280 AT3G63060.1 42.534 221 125 2 23 242 36 255 3.90e-63 199

Find 126 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAATATTCAACTAAA+AGG 0.164816 7_4:-5998176 None:intergenic
TAGTGTTTCATAATTTAAAA+TGG 0.209786 7_4:+5998491 Msa1137280:CDS
CACAACTTCGCTTTACAATA+AGG 0.238798 7_4:-5998976 None:intergenic
AACGATAATGCGACTTTCAT+TGG 0.271652 7_4:+5997246 Msa1137280:five_prime_UTR
CAAGGTGAAGAAGGAGATAT+TGG 0.288349 7_4:+5998859 Msa1137280:CDS
TTCAACTATTACTCCAATTC+AGG 0.290810 7_4:-5999144 None:intergenic
CAGCCCAAACCCATCATTAA+TGG 0.299946 7_4:-5997445 None:intergenic
GATGGGAACTGGAGGGCATT+AGG 0.301055 7_4:+5998655 Msa1137280:CDS
AGGAAAATGGTGAGCCTCTT+GGG 0.302515 7_4:+6000303 Msa1137280:three_prime_UTR
TGCAGATCACACAACATCTT+TGG 0.305769 7_4:-5998610 None:intergenic
CAGATTATTTACAAGTTGTT+TGG 0.310423 7_4:-6000694 None:intergenic
AAGACTGTTTGTGGGGCTTA+TGG 0.311944 7_4:+5997504 Msa1137280:five_prime_UTR
ACTTATTTCCATACGCTTGC+AGG 0.313651 7_4:+5997560 Msa1137280:five_prime_UTR
AGCTAATATGAGGGGCAATT+TGG 0.322687 7_4:-5996457 None:intergenic
TTGTGAATATTTGAACTCTT+AGG 0.332759 7_4:+5997750 Msa1137280:five_prime_UTR
CAGAATCAGACAACGGCAAT+AGG 0.334261 7_4:-5999113 None:intergenic
TTCAAGATGAAGAAGGAAAA+TGG 0.337135 7_4:+6000290 Msa1137280:three_prime_UTR
TTCTGAAGAGGCGCCTGAAT+TGG 0.352655 7_4:+5999131 Msa1137280:CDS
ACATCCATTAATGATGGGTT+TGG 0.356850 7_4:+5997441 Msa1137280:five_prime_UTR
CTCTATCATGTGTGTGCAAA+TGG 0.361648 7_4:+5998533 Msa1137280:CDS
CTACAAATTTACAATATGTT+TGG 0.363437 7_4:-5997999 None:intergenic
ACGATTATTTACATCATGTT+TGG 0.365570 7_4:-6000655 None:intergenic
TGCTTCCAGCAGTGTGTTGT+GGG 0.366169 7_4:-5998100 None:intergenic
CAAAGTGATTGAGTATTGTT+TGG 0.369583 7_4:+5997839 Msa1137280:five_prime_UTR
AATGTCTGGTTGGTATGCTT+TGG 0.390732 7_4:+6000505 Msa1137280:three_prime_UTR
ACAACTTCGCTTTACAATAA+GGG 0.398128 7_4:-5998975 None:intergenic
TGCCTCAATGGTCACTTGAT+TGG 0.401501 7_4:+5999081 Msa1137280:CDS
CAAAGTGCTAGCTGCAGGTT+GGG 0.401792 7_4:+5999027 Msa1137280:CDS
TGTATGTTGCTGCTAAAATT+AGG 0.405550 7_4:+5997803 Msa1137280:five_prime_UTR
AATACAACACCTCCAATAGA+TGG 0.406390 7_4:-6000450 None:intergenic
AATTTACAATATGTTTGGTT+TGG 0.410012 7_4:-5997994 None:intergenic
GCCACGTCGTTGATGGGAAC+TGG 0.411747 7_4:+5998644 Msa1137280:CDS
TTGTGTGATCTGCAATCAAC+TGG 0.417058 7_4:+5998619 Msa1137280:CDS
AAGCTTCTTACGTACTGTTC+GGG 0.422997 7_4:+5998679 Msa1137280:CDS
ACTGGGAGCCACGTCGTTGA+TGG 0.424116 7_4:+5998637 Msa1137280:CDS
CATCCATTAATGATGGGTTT+GGG 0.424733 7_4:+5997442 Msa1137280:five_prime_UTR
ATTGGGTTTCTAACTCCGGC+TGG 0.427801 7_4:+5997264 Msa1137280:five_prime_UTR
ATGGGAACTGGAGGGCATTA+GGG 0.429718 7_4:+5998656 Msa1137280:CDS
ATCTTCACTTAGATGCCCTT+TGG 0.429745 7_4:-5998458 None:intergenic
GAAGCTTCTTACGTACTGTT+CGG 0.432478 7_4:+5998678 Msa1137280:CDS
CTAAACACATCCATTAATGA+TGG 0.436220 7_4:+5997435 Msa1137280:five_prime_UTR
TTCTGTAATTATACTTGTGA+TGG 0.437144 7_4:+5997940 Msa1137280:five_prime_UTR
ACGATAATGCGACTTTCATT+GGG 0.438422 7_4:+5997247 Msa1137280:five_prime_UTR
AACCTGCAGCTAGCACTTTG+AGG 0.444997 7_4:-5999024 None:intergenic
TTGTACCGATAGCAATTCCT+TGG 0.446337 7_4:+5997390 Msa1137280:five_prime_UTR
TGATATCTTTCCTCTAATGA+AGG 0.451969 7_4:+5997359 Msa1137280:five_prime_UTR
CTGCTTCCAGCAGTGTGTTG+TGG 0.455287 7_4:-5998101 None:intergenic
GCCTCAATGGTCACTTGATT+GGG 0.456870 7_4:+5999082 Msa1137280:CDS
CATCTAGATCAAGGTGAAGA+AGG 0.463015 7_4:+5998850 Msa1137280:CDS
CTCTTTCCATAGAACTCGCT+TGG 0.463797 7_4:-5998569 None:intergenic
GCTTGATGTGCAACGTCAAA+CGG 0.468341 7_4:-5997206 None:intergenic
AAGGAAAATGGTGAGCCTCT+TGG 0.469339 7_4:+6000302 Msa1137280:three_prime_UTR
AAAACACCGTCCTTCATTAG+AGG 0.473973 7_4:-5997369 None:intergenic
ATCTTGCCAAGCGAGTTCTA+TGG 0.476286 7_4:+5998563 Msa1137280:CDS
AAATTAATATTCAACTAAAA+GGG 0.477316 7_4:-5998175 None:intergenic
CATTAATGATGGGTTTGGGC+TGG 0.477597 7_4:+5997446 Msa1137280:five_prime_UTR
ATGTAGCATGATCCATCTAT+TGG 0.479951 7_4:+6000438 Msa1137280:three_prime_UTR
CATCTTGAATTTAGCAGAGT+CGG 0.482579 7_4:-6000276 None:intergenic
AACTTGTGTAAGACTGTTTG+TGG 0.486725 7_4:+5997495 Msa1137280:five_prime_UTR
TCAAAGTGCTAGCTGCAGGT+TGG 0.489268 7_4:+5999026 Msa1137280:CDS
TGTACCGATAGCAATTCCTT+GGG 0.492322 7_4:+5997391 Msa1137280:five_prime_UTR
AGAATCAGACAACGGCAATA+GGG 0.497429 7_4:-5999112 None:intergenic
CAAACAATACTCAATCACTT+TGG 0.501113 7_4:-5997838 None:intergenic
TGAAACCCACAACACACTGC+TGG 0.504466 7_4:+5998095 Msa1137280:five_prime_UTR
CTGGGAGCCACGTCGTTGAT+GGG 0.504476 7_4:+5998638 Msa1137280:CDS
TTTCATTGGGTTTCTAACTC+CGG 0.505320 7_4:+5997260 Msa1137280:five_prime_UTR
AAATTGCCCCTCATATTAGC+TGG 0.506482 7_4:+5996459 Msa1137280:five_prime_UTR
GTGAAGAATACTCATAGTAT+AGG 0.509863 7_4:+6000400 Msa1137280:three_prime_UTR
TATGTAAGAACACTTATGCT+TGG 0.512763 7_4:+6000612 Msa1137280:three_prime_UTR
GATAGAGCAGCAATCAGCTT+AGG 0.512808 7_4:-5998517 None:intergenic
TAAACACATCCATTAATGAT+GGG 0.518097 7_4:+5997436 Msa1137280:five_prime_UTR
TTTGACGTTGCACATCAAGC+AGG 0.518359 7_4:+5997209 Msa1137280:five_prime_UTR
TTATTTACAAGTTGTTTGGA+TGG 0.527757 7_4:-6000690 None:intergenic
AGAAAGATGCTTATTAGTAA+AGG 0.529828 7_4:+5998925 Msa1137280:CDS
AACTTGTAAATAATCTGACT+TGG 0.531224 7_4:+6000700 Msa1137280:three_prime_UTR
TTATTTACATCATGTTTGGA+TGG 0.531853 7_4:-6000651 None:intergenic
CTTCAATTTCCACTCAGAGG+TGG 0.535038 7_4:+5996546 Msa1137280:five_prime_UTR
ACAAACTATACTGATTATGT+TGG 0.537557 7_4:+6000723 Msa1137280:three_prime_UTR
TGCTAAATTCAAGATGAAGA+AGG 0.540146 7_4:+6000283 Msa1137280:three_prime_UTR
TTCCTCAAAGTGCTAGCTGC+AGG 0.540338 7_4:+5999022 Msa1137280:CDS
TTAACGATCCTGCAAGCGTA+TGG 0.541472 7_4:-5997568 None:intergenic
TTCAATTTCCACTCAGAGGT+GGG 0.542737 7_4:+5996547 Msa1137280:five_prime_UTR
ACTAAAAGGGTTGCAATCCA+TGG 0.544566 7_4:-5998162 None:intergenic
ATTTGGTCAAGAAATGAGTA+AGG 0.545610 7_4:-5996440 None:intergenic
AACTCAACACTTCTCCCAGC+CGG 0.547403 7_4:-5997279 None:intergenic
GCCGTTGTCTGATTCTGAAG+AGG 0.548453 7_4:+5999119 Msa1137280:CDS
AACATTCAGAATCAGATCCC+TGG 0.551777 7_4:+5998727 Msa1137280:CDS
TTGGGTTTCTAACTCCGGCT+GGG 0.554045 7_4:+5997265 Msa1137280:five_prime_UTR
TAGATGCAGATCACTTGTGC+AGG 0.554067 7_4:+6000378 Msa1137280:three_prime_UTR
CCTTGTGAGCATCTAGATCA+AGG 0.554478 7_4:+5998841 Msa1137280:CDS
ACTTGTGTAAGACTGTTTGT+GGG 0.554723 7_4:+5997496 Msa1137280:five_prime_UTR
GTCTGATACACAAAGTGACC+AGG 0.560165 7_4:-5998745 None:intergenic
CTAGCTGTCAATGTTCCAAA+GGG 0.560254 7_4:+5998443 Msa1137280:CDS
CCTTGATCTAGATGCTCACA+AGG 0.564697 7_4:-5998841 None:intergenic
TGATGTGCTATCGCGTATGT+TGG 0.566092 7_4:+6000334 Msa1137280:three_prime_UTR
TTCTTGGTTAGCAAACATCA+TGG 0.570595 7_4:-5998336 None:intergenic
GCTAGCTGTCAATGTTCCAA+AGG 0.578026 7_4:+5998442 Msa1137280:CDS
TAAGTCGTTTGCAACGTCAA+AGG 0.578884 7_4:+5998900 Msa1137280:CDS
TTAAGCAGTTTATCTAAACA+TGG 0.585305 7_4:+5997704 Msa1137280:five_prime_UTR
CGTTACACTGAACAACAAGC+TGG 0.585680 7_4:-5997155 None:intergenic
TTGACGTTGCACATCAAGCA+GGG 0.587625 7_4:+5997210 Msa1137280:five_prime_UTR
TAGCATGATCCATCTATTGG+AGG 0.588497 7_4:+6000441 Msa1137280:three_prime_UTR
ATCTTTCCTCTAATGAAGGA+CGG 0.589704 7_4:+5997363 Msa1137280:five_prime_UTR
CTTATTGTAAAGCGAAGTTG+TGG 0.591906 7_4:+5998977 Msa1137280:CDS
ACGTCGTTGATGGGAACTGG+AGG 0.594238 7_4:+5998647 Msa1137280:CDS
TACTCATAGTATAGGTGACT+AGG 0.595516 7_4:+6000408 Msa1137280:three_prime_UTR
AAATGGTGAGCCTCTTGGGG+TGG 0.596335 7_4:+6000307 Msa1137280:three_prime_UTR
TATACCCAAGGAATTGCTAT+CGG 0.597827 7_4:-5997395 None:intergenic
TTTGTAAGCACGATATCATG+AGG 0.601873 7_4:-6000774 None:intergenic
TAACTTCAATTTCCACTCAG+AGG 0.610940 7_4:+5996543 Msa1137280:five_prime_UTR
AAAACATCAGTTCTGCATTG+TGG 0.613771 7_4:-5998805 None:intergenic
AGCTAGCTGAATGTATGCAT+CGG 0.619048 7_4:-5998426 None:intergenic
TGTTCGGGATGCACGAAAGG+TGG 0.621782 7_4:+5998694 Msa1137280:CDS
CTTGTGTAAGACTGTTTGTG+GGG 0.622230 7_4:+5997497 Msa1137280:five_prime_UTR
GGAAAATGGTGAGCCTCTTG+GGG 0.625476 7_4:+6000304 Msa1137280:three_prime_UTR
GGAGATATTGGATTCTTCCG+CGG 0.632203 7_4:+5998871 Msa1137280:CDS
ACATCATAAACCACCCCAAG+AGG 0.632395 7_4:-6000317 None:intergenic
CACATGAGGGAAATAAAACA+AGG 0.636730 7_4:+5997911 Msa1137280:five_prime_UTR
TCCAGTTCCCATCAACGACG+TGG 0.656887 7_4:-5998645 None:intergenic
CGTCGTTGATGGGAACTGGA+GGG 0.667520 7_4:+5998648 Msa1137280:CDS
TCCCAATCAAGTGACCATTG+AGG 0.669913 7_4:-5999083 None:intergenic
GCCTCTTCAGAATCAGACAA+CGG 0.682457 7_4:-5999120 None:intergenic
GAACTGGTTTGTGAGTACCA+TGG 0.688393 7_4:+5998145 Msa1137280:five_prime_UTR
TCTGATACACAAAGTGACCA+GGG 0.703260 7_4:-5998744 None:intergenic
TGTGTGATCTGCAATCAACT+GGG 0.707515 7_4:+5998620 Msa1137280:CDS
GTGCAACGTCAAACGGCATG+TGG 0.713160 7_4:-5997199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATATTCAACTAAAA+GGG - chr7_4:5998178-5998197 None:intergenic 10.0%
!! AAAATTAATATTCAACTAAA+AGG - chr7_4:5998179-5998198 None:intergenic 10.0%
!! TATCAGTTTATAAAAATTCT+TGG - chr7_4:5998355-5998374 None:intergenic 15.0%
!! TAGTGTTTCATAATTTAAAA+TGG + chr7_4:5998491-5998510 Msa1137280:CDS 15.0%
!!! TTTATTTTTTATTTCGTGTA+AGG + chr7_4:5999908-5999927 Msa1137280:intron 15.0%
!!! TGATTTTATTCGATTTGTAT+TGG + chr7_4:5996633-5996652 Msa1137280:intron 20.0%
!!! TTGATTTGCATATAATTGAT+GGG + chr7_4:5996707-5996726 Msa1137280:intron 20.0%
!!! AAAGATATTTTTGTAGAAGA+GGG + chr7_4:5997315-5997334 Msa1137280:five_prime_UTR 20.0%
!!! TAATATTTTAAGAGCTTGTT+TGG + chr7_4:5997873-5997892 Msa1137280:five_prime_UTR 20.0%
!!! AATATTTTAAGAGCTTGTTT+GGG + chr7_4:5997874-5997893 Msa1137280:five_prime_UTR 20.0%
!! AATTTACAATATGTTTGGTT+TGG - chr7_4:5997997-5998016 None:intergenic 20.0%
!! CTACAAATTTACAATATGTT+TGG - chr7_4:5998002-5998021 None:intergenic 20.0%
!!! TGTTTTGCTTAAGTATATAT+TGG + chr7_4:5998198-5998217 Msa1137280:five_prime_UTR 20.0%
!!! ATTTTTCTTTTAGTTTTGCT+AGG + chr7_4:5998375-5998394 Msa1137280:five_prime_UTR 20.0%
!! AACAGAAAAATAGTAAGTAA+TGG - chr7_4:5999202-5999221 None:intergenic 20.0%
!! ATAATCTATGATTTCTTTCT+AGG + chr7_4:5999322-5999341 Msa1137280:intron 20.0%
!! TTTAGACCTAATAATTGATA+GGG - chr7_4:5999381-5999400 None:intergenic 20.0%
!! AAAAGATAGTTAGAAGTAAA+TGG - chr7_4:5999410-5999429 None:intergenic 20.0%
!!! TTTAAAACATGTCTTTTAGA+GGG - chr7_4:5999593-5999612 None:intergenic 20.0%
!!! ATTTAAAACATGTCTTTTAG+AGG - chr7_4:5999594-5999613 None:intergenic 20.0%
!!! ATTTGCTAGTTATATATGTA+AGG + chr7_4:6000107-6000126 Msa1137280:intron 20.0%
!!! ATGTGATTAAATTAGTTTGA+AGG + chr7_4:6000205-6000224 Msa1137280:intron 20.0%
!!! ATTCTATTATTTTCAATGTC+TGG + chr7_4:6000491-6000510 Msa1137280:three_prime_UTR 20.0%
!!! CTTGATTTGCATATAATTGA+TGG + chr7_4:5996706-5996725 Msa1137280:intron 25.0%
! TATAATTGATGGGAAATTTC+TGG + chr7_4:5996717-5996736 Msa1137280:intron 25.0%
! ATAATTGATGGGAAATTTCT+GGG + chr7_4:5996718-5996737 Msa1137280:intron 25.0%
!!! CAAAGATATTTTTGTAGAAG+AGG + chr7_4:5997314-5997333 Msa1137280:five_prime_UTR 25.0%
! TGATTACAAAAATATACCCA+AGG - chr7_4:5997410-5997429 None:intergenic 25.0%
! TAAACACATCCATTAATGAT+GGG + chr7_4:5997436-5997455 Msa1137280:five_prime_UTR 25.0%
! TTAAGCAGTTTATCTAAACA+TGG + chr7_4:5997704-5997723 Msa1137280:five_prime_UTR 25.0%
! TTGTGAATATTTGAACTCTT+AGG + chr7_4:5997750-5997769 Msa1137280:five_prime_UTR 25.0%
!!! ATATTTTAAGAGCTTGTTTG+GGG + chr7_4:5997875-5997894 Msa1137280:five_prime_UTR 25.0%
!! ACAAAATTTTGAACACATGA+GGG + chr7_4:5997898-5997917 Msa1137280:five_prime_UTR 25.0%
! TTCTGTAATTATACTTGTGA+TGG + chr7_4:5997940-5997959 Msa1137280:five_prime_UTR 25.0%
!! GTAGTCTTCATTTTCATTTT+TGG + chr7_4:5998018-5998037 Msa1137280:five_prime_UTR 25.0%
!!! TCATTTTTGTTTTCTTGAAC+TGG + chr7_4:5998129-5998148 Msa1137280:five_prime_UTR 25.0%
! AGAAAGATGCTTATTAGTAA+AGG + chr7_4:5998925-5998944 Msa1137280:CDS 25.0%
!! GTAATAGTTGAATGATTTTC+AGG + chr7_4:5999155-5999174 Msa1137280:exon 25.0%
! GTAAATCCCTATCAATTATT+AGG + chr7_4:5999372-5999391 Msa1137280:intron 25.0%
! CTTTAGACCTAATAATTGAT+AGG - chr7_4:5999382-5999401 None:intergenic 25.0%
!! CTAACTATCTTTTTCTGATT+TGG + chr7_4:5999417-5999436 Msa1137280:intron 25.0%
!! AAAATCAAAGACAAGAGTAT+AGG - chr7_4:5999464-5999483 None:intergenic 25.0%
! AATCATTAGAGAGAAAAAGA+TGG - chr7_4:5999668-5999687 None:intergenic 25.0%
! AAATTGTATGTTGAATTCAG+TGG + chr7_4:5999839-5999858 Msa1137280:intron 25.0%
!! CTCATTAGATATTTTGCAAA+TGG + chr7_4:5999955-5999974 Msa1137280:intron 25.0%
! AATTACTATAATTGCACTAG+TGG + chr7_4:5999990-6000009 Msa1137280:intron 25.0%
! AGGATGTATCTGATTATATT+TGG + chr7_4:6000127-6000146 Msa1137280:intron 25.0%
!! TATTATTTTCAATGTCTGGT+TGG + chr7_4:6000495-6000514 Msa1137280:three_prime_UTR 25.0%
! TTATTTACATCATGTTTGGA+TGG - chr7_4:6000654-6000673 None:intergenic 25.0%
! ACGATTATTTACATCATGTT+TGG - chr7_4:6000658-6000677 None:intergenic 25.0%
! TTATTTACAAGTTGTTTGGA+TGG - chr7_4:6000693-6000712 None:intergenic 25.0%
! CAGATTATTTACAAGTTGTT+TGG - chr7_4:6000697-6000716 None:intergenic 25.0%
! AACTTGTAAATAATCTGACT+TGG + chr7_4:6000700-6000719 Msa1137280:three_prime_UTR 25.0%
!! ACAAACTATACTGATTATGT+TGG + chr7_4:6000723-6000742 Msa1137280:three_prime_UTR 25.0%
ATTTGGTCAAGAAATGAGTA+AGG - chr7_4:5996443-5996462 None:intergenic 30.0%
! AATTTTCCAGCTAATATGAG+GGG - chr7_4:5996468-5996487 None:intergenic 30.0%
! CAATTTTCCAGCTAATATGA+GGG - chr7_4:5996469-5996488 None:intergenic 30.0%
TTATAGCTAGCTGATAACTT+TGG + chr7_4:5996867-5996886 Msa1137280:intron 30.0%
ATCAGCAGTCAATTAATTGA+TGG + chr7_4:5996914-5996933 Msa1137280:intron 30.0%
TCAGCAGTCAATTAATTGAT+GGG + chr7_4:5996915-5996934 Msa1137280:intron 30.0%
TGATATCTTTCCTCTAATGA+AGG + chr7_4:5997359-5997378 Msa1137280:five_prime_UTR 30.0%
CTAAACACATCCATTAATGA+TGG + chr7_4:5997435-5997454 Msa1137280:five_prime_UTR 30.0%
! TGGCTTTTATGTATGATTTG+AGG + chr7_4:5997724-5997743 Msa1137280:five_prime_UTR 30.0%
! GGCTTTTATGTATGATTTGA+GGG + chr7_4:5997725-5997744 Msa1137280:five_prime_UTR 30.0%
TGTATGTTGCTGCTAAAATT+AGG + chr7_4:5997803-5997822 Msa1137280:five_prime_UTR 30.0%
CAAACAATACTCAATCACTT+TGG - chr7_4:5997841-5997860 None:intergenic 30.0%
! CAAAGTGATTGAGTATTGTT+TGG + chr7_4:5997839-5997858 Msa1137280:five_prime_UTR 30.0%
! GACAAAATTTTGAACACATG+AGG + chr7_4:5997897-5997916 Msa1137280:five_prime_UTR 30.0%
!! GTGTTTGAGTTTTGTCTATT+TGG + chr7_4:5998307-5998326 Msa1137280:five_prime_UTR 30.0%
ACAACTTCGCTTTACAATAA+GGG - chr7_4:5998978-5998997 None:intergenic 30.0%
TTCAACTATTACTCCAATTC+AGG - chr7_4:5999147-5999166 None:intergenic 30.0%
!! TTCAGGTATTTTTGTGTATC+TGG + chr7_4:5999172-5999191 Msa1137280:intron 30.0%
!! CTTTACTTTTCTTTGAGAAC+TGG + chr7_4:5999225-5999244 Msa1137280:intron 30.0%
TCTATGATTTCTTTCTAGGT+AGG + chr7_4:5999326-5999345 Msa1137280:intron 30.0%
CTATGATTTCTTTCTAGGTA+GGG + chr7_4:5999327-5999346 Msa1137280:intron 30.0%
GGAAGACTAAGGAAAAATAT+GGG - chr7_4:5999443-5999462 None:intergenic 30.0%
AGGAAGACTAAGGAAAAATA+TGG - chr7_4:5999444-5999463 None:intergenic 30.0%
!! TCATAGTTTTCTGTTGCTTA+GGG - chr7_4:5999535-5999554 None:intergenic 30.0%
!! TTCATAGTTTTCTGTTGCTT+AGG - chr7_4:5999536-5999555 None:intergenic 30.0%
AATGCTACACAAGATAAGAT+AGG - chr7_4:5999702-5999721 None:intergenic 30.0%
AATATTAGCATGTGATACAC+AGG - chr7_4:5999887-5999906 None:intergenic 30.0%
TGTTCAAAACAGTTTGACAA+TGG - chr7_4:6000036-6000055 None:intergenic 30.0%
AATGATTAGCAACTATCTTC+TGG + chr7_4:6000152-6000171 Msa1137280:intron 30.0%
ATACAATTAACCTGTTACTG+AGG - chr7_4:6000180-6000199 None:intergenic 30.0%
TGCTAAATTCAAGATGAAGA+AGG + chr7_4:6000283-6000302 Msa1137280:three_prime_UTR 30.0%
TTCAAGATGAAGAAGGAAAA+TGG + chr7_4:6000290-6000309 Msa1137280:three_prime_UTR 30.0%
GTGAAGAATACTCATAGTAT+AGG + chr7_4:6000400-6000419 Msa1137280:three_prime_UTR 30.0%
TATGTAAGAACACTTATGCT+TGG + chr7_4:6000612-6000631 Msa1137280:three_prime_UTR 30.0%
! GCAATTTTCCAGCTAATATG+AGG - chr7_4:5996470-5996489 None:intergenic 35.0%
TAACTTCAATTTCCACTCAG+AGG + chr7_4:5996543-5996562 Msa1137280:five_prime_UTR 35.0%
TTCTGGGTGATCTATGATAT+TGG + chr7_4:5996734-5996753 Msa1137280:intron 35.0%
TCTGGGTGATCTATGATATT+GGG + chr7_4:5996735-5996754 Msa1137280:intron 35.0%
GTTACTGTCATTAAATGTCG+CGG + chr7_4:5996989-5997008 Msa1137280:intron 35.0%
AGCTACAACAGAGAAAAATG+TGG - chr7_4:5997100-5997119 None:intergenic 35.0%
AACGATAATGCGACTTTCAT+TGG + chr7_4:5997246-5997265 Msa1137280:five_prime_UTR 35.0%
ACGATAATGCGACTTTCATT+GGG + chr7_4:5997247-5997266 Msa1137280:five_prime_UTR 35.0%
TTTCATTGGGTTTCTAACTC+CGG + chr7_4:5997260-5997279 Msa1137280:five_prime_UTR 35.0%
ATCTTTCCTCTAATGAAGGA+CGG + chr7_4:5997363-5997382 Msa1137280:five_prime_UTR 35.0%
TATACCCAAGGAATTGCTAT+CGG - chr7_4:5997398-5997417 None:intergenic 35.0%
!! ACATCCATTAATGATGGGTT+TGG + chr7_4:5997441-5997460 Msa1137280:five_prime_UTR 35.0%
!! CATCCATTAATGATGGGTTT+GGG + chr7_4:5997442-5997461 Msa1137280:five_prime_UTR 35.0%
AACTTGTGTAAGACTGTTTG+TGG + chr7_4:5997495-5997514 Msa1137280:five_prime_UTR 35.0%
ACTTGTGTAAGACTGTTTGT+GGG + chr7_4:5997496-5997515 Msa1137280:five_prime_UTR 35.0%
CACATGAGGGAAATAAAACA+AGG + chr7_4:5997911-5997930 Msa1137280:five_prime_UTR 35.0%
TTCTTGGTTAGCAAACATCA+TGG - chr7_4:5998339-5998358 None:intergenic 35.0%
!!! ACTCGATTTTCTAGAACATC+AGG + chr7_4:5998766-5998785 Msa1137280:CDS 35.0%
!!! CTCGATTTTCTAGAACATCA+GGG + chr7_4:5998767-5998786 Msa1137280:CDS 35.0%
AAAACATCAGTTCTGCATTG+TGG - chr7_4:5998808-5998827 None:intergenic 35.0%
CACAACTTCGCTTTACAATA+AGG - chr7_4:5998979-5998998 None:intergenic 35.0%
CTTATTGTAAAGCGAAGTTG+TGG + chr7_4:5998977-5998996 Msa1137280:CDS 35.0%
! GATCCATTTTGAATGTTAGC+AGG + chr7_4:5999247-5999266 Msa1137280:intron 35.0%
CTACCTGCTAACATTCAAAA+TGG - chr7_4:5999253-5999272 None:intergenic 35.0%
! ACAAGAGTATAGGAAGACTA+AGG - chr7_4:5999454-5999473 None:intergenic 35.0%
! GATTTTCTGACTTTTCCACA+TGG + chr7_4:5999478-5999497 Msa1137280:intron 35.0%
!! ATAGTTTTCTGTTGCTTAGG+GGG - chr7_4:5999533-5999552 None:intergenic 35.0%
!! CATAGTTTTCTGTTGCTTAG+GGG - chr7_4:5999534-5999553 None:intergenic 35.0%
CATCAAGCGAAAAAAAGTTG+AGG - chr7_4:5999619-5999638 None:intergenic 35.0%
AGATGGTACAATCTCATCTT+AGG - chr7_4:5999651-5999670 None:intergenic 35.0%
CATGTGATACACAGGAAAAA+AGG - chr7_4:5999879-5999898 None:intergenic 35.0%
ATTGAGTCAATGGTTAGTGA+TGG + chr7_4:5999931-5999950 Msa1137280:intron 35.0%
AGCAAATAGAGAACAGTAGA+AGG - chr7_4:6000094-6000113 None:intergenic 35.0%
CATCTTGAATTTAGCAGAGT+CGG - chr7_4:6000279-6000298 None:intergenic 35.0%
TATCGCGTATGTTGGAAAAA+TGG + chr7_4:6000342-6000361 Msa1137280:three_prime_UTR 35.0%
TACTCATAGTATAGGTGACT+AGG + chr7_4:6000408-6000427 Msa1137280:three_prime_UTR 35.0%
ATGTAGCATGATCCATCTAT+TGG + chr7_4:6000438-6000457 Msa1137280:three_prime_UTR 35.0%
AATACAACACCTCCAATAGA+TGG - chr7_4:6000453-6000472 None:intergenic 35.0%
AGCTAATATGAGGGGCAATT+TGG - chr7_4:5996460-5996479 None:intergenic 40.0%
AAATTGCCCCTCATATTAGC+TGG + chr7_4:5996459-5996478 Msa1137280:five_prime_UTR 40.0%
TTCAATTTCCACTCAGAGGT+GGG + chr7_4:5996547-5996566 Msa1137280:five_prime_UTR 40.0%
GTGATCTATGATATTGGGAC+TGG + chr7_4:5996740-5996759 Msa1137280:intron 40.0%
AAAACACCGTCCTTCATTAG+AGG - chr7_4:5997372-5997391 None:intergenic 40.0%
! TTGTACCGATAGCAATTCCT+TGG + chr7_4:5997390-5997409 Msa1137280:five_prime_UTR 40.0%
! TGTACCGATAGCAATTCCTT+GGG + chr7_4:5997391-5997410 Msa1137280:five_prime_UTR 40.0%
CTTGTGTAAGACTGTTTGTG+GGG + chr7_4:5997497-5997516 Msa1137280:five_prime_UTR 40.0%
ACTTATTTCCATACGCTTGC+AGG + chr7_4:5997560-5997579 Msa1137280:five_prime_UTR 40.0%
ACTAAAAGGGTTGCAATCCA+TGG - chr7_4:5998165-5998184 None:intergenic 40.0%
AGCTAGCTGAATGTATGCAT+CGG - chr7_4:5998429-5998448 None:intergenic 40.0%
CTAGCTGTCAATGTTCCAAA+GGG + chr7_4:5998443-5998462 Msa1137280:CDS 40.0%
ATCTTCACTTAGATGCCCTT+TGG - chr7_4:5998461-5998480 None:intergenic 40.0%
CTCTATCATGTGTGTGCAAA+TGG + chr7_4:5998533-5998552 Msa1137280:CDS 40.0%
TGCAGATCACACAACATCTT+TGG - chr7_4:5998613-5998632 None:intergenic 40.0%
TTGTGTGATCTGCAATCAAC+TGG + chr7_4:5998619-5998638 Msa1137280:CDS 40.0%
TGTGTGATCTGCAATCAACT+GGG + chr7_4:5998620-5998639 Msa1137280:CDS 40.0%
GAAGCTTCTTACGTACTGTT+CGG + chr7_4:5998678-5998697 Msa1137280:CDS 40.0%
AAGCTTCTTACGTACTGTTC+GGG + chr7_4:5998679-5998698 Msa1137280:CDS 40.0%
AACATTCAGAATCAGATCCC+TGG + chr7_4:5998727-5998746 Msa1137280:CDS 40.0%
TCTGATACACAAAGTGACCA+GGG - chr7_4:5998747-5998766 None:intergenic 40.0%
CATCTAGATCAAGGTGAAGA+AGG + chr7_4:5998850-5998869 Msa1137280:CDS 40.0%
CAAGGTGAAGAAGGAGATAT+TGG + chr7_4:5998859-5998878 Msa1137280:CDS 40.0%
CAAACGACTTAAAAACTCCG+CGG - chr7_4:5998891-5998910 None:intergenic 40.0%
TAAGTCGTTTGCAACGTCAA+AGG + chr7_4:5998900-5998919 Msa1137280:CDS 40.0%
! GAGTATTTTGTGTGCCTCAA+TGG + chr7_4:5999069-5999088 Msa1137280:CDS 40.0%
AGAATCAGACAACGGCAATA+GGG - chr7_4:5999115-5999134 None:intergenic 40.0%
GATTTCTTTCTAGGTAGGGT+CGG + chr7_4:5999331-5999350 Msa1137280:intron 40.0%
!! CATGTCTTTTAGAGGGAATG+CGG - chr7_4:5999586-5999605 None:intergenic 40.0%
TGGTACAATCTCATCTTAGG+TGG - chr7_4:5999648-5999667 None:intergenic 40.0%
CTCAAGTAATGTCTTACTCG+AGG - chr7_4:5999818-5999837 None:intergenic 40.0%
TCGTGTAAGGATTGAGTCAA+TGG + chr7_4:5999921-5999940 Msa1137280:intron 40.0%
ATAGAGAACAGTAGAAGGTC+TGG - chr7_4:6000089-6000108 None:intergenic 40.0%
TAGCATGATCCATCTATTGG+AGG + chr7_4:6000441-6000460 Msa1137280:three_prime_UTR 40.0%
! AATGTCTGGTTGGTATGCTT+TGG + chr7_4:6000505-6000524 Msa1137280:three_prime_UTR 40.0%
CTTCAATTTCCACTCAGAGG+TGG + chr7_4:5996546-5996565 Msa1137280:five_prime_UTR 45.0%
TAGAAAAACCCACCTCTGAG+TGG - chr7_4:5996558-5996577 None:intergenic 45.0%
!! ACTCAGAGGTGGGTTTTTCT+AGG + chr7_4:5996557-5996576 Msa1137280:five_prime_UTR 45.0%
!! CTCAGAGGTGGGTTTTTCTA+GGG + chr7_4:5996558-5996577 Msa1137280:five_prime_UTR 45.0%
!!! TGGGTTTTTCTAGGGTTTCG+AGG + chr7_4:5996566-5996585 Msa1137280:five_prime_UTR 45.0%
!! GATATTGGGACTGGTTGATG+TGG + chr7_4:5996749-5996768 Msa1137280:intron 45.0%
! GGTCATTGATTGCAGACGAA+AGG + chr7_4:5997010-5997029 Msa1137280:intron 45.0%
CGTTACACTGAACAACAAGC+TGG - chr7_4:5997158-5997177 None:intergenic 45.0%
GCTTGATGTGCAACGTCAAA+CGG - chr7_4:5997209-5997228 None:intergenic 45.0%
TTTGACGTTGCACATCAAGC+AGG + chr7_4:5997209-5997228 Msa1137280:five_prime_UTR 45.0%
TTGACGTTGCACATCAAGCA+GGG + chr7_4:5997210-5997229 Msa1137280:five_prime_UTR 45.0%
CAGCCCAAACCCATCATTAA+TGG - chr7_4:5997448-5997467 None:intergenic 45.0%
!! CATTAATGATGGGTTTGGGC+TGG + chr7_4:5997446-5997465 Msa1137280:five_prime_UTR 45.0%
AAGACTGTTTGTGGGGCTTA+TGG + chr7_4:5997504-5997523 Msa1137280:five_prime_UTR 45.0%
TTAACGATCCTGCAAGCGTA+TGG - chr7_4:5997571-5997590 None:intergenic 45.0%
!! GAACTGGTTTGTGAGTACCA+TGG + chr7_4:5998145-5998164 Msa1137280:five_prime_UTR 45.0%
GCTAGCTGTCAATGTTCCAA+AGG + chr7_4:5998442-5998461 Msa1137280:CDS 45.0%
GATAGAGCAGCAATCAGCTT+AGG - chr7_4:5998520-5998539 None:intergenic 45.0%
ATCTTGCCAAGCGAGTTCTA+TGG + chr7_4:5998563-5998582 Msa1137280:CDS 45.0%
CTCTTTCCATAGAACTCGCT+TGG - chr7_4:5998572-5998591 None:intergenic 45.0%
GTCTGATACACAAAGTGACC+AGG - chr7_4:5998748-5998767 None:intergenic 45.0%
CCTTGATCTAGATGCTCACA+AGG - chr7_4:5998844-5998863 None:intergenic 45.0%
CCTTGTGAGCATCTAGATCA+AGG + chr7_4:5998841-5998860 Msa1137280:CDS 45.0%
GGAGATATTGGATTCTTCCG+CGG + chr7_4:5998871-5998890 Msa1137280:CDS 45.0%
TGCCTCAATGGTCACTTGAT+TGG + chr7_4:5999081-5999100 Msa1137280:CDS 45.0%
GCCTCAATGGTCACTTGATT+GGG + chr7_4:5999082-5999101 Msa1137280:CDS 45.0%
TCCCAATCAAGTGACCATTG+AGG - chr7_4:5999086-5999105 None:intergenic 45.0%
CAGAATCAGACAACGGCAAT+AGG - chr7_4:5999116-5999135 None:intergenic 45.0%
GCCTCTTCAGAATCAGACAA+CGG - chr7_4:5999123-5999142 None:intergenic 45.0%
TCTAGGTAGGGTCGGTTATA+TGG + chr7_4:5999339-5999358 Msa1137280:intron 45.0%
GGAACCATGCATAGCTCAAT+AGG + chr7_4:5999499-5999518 Msa1137280:intron 45.0%
CACTCCTATTGAGCTATGCA+TGG - chr7_4:5999506-5999525 None:intergenic 45.0%
CATGCATAGCTCAATAGGAG+TGG + chr7_4:5999504-5999523 Msa1137280:intron 45.0%
TCTTCTGGTACCTCAGTAAC+AGG + chr7_4:6000167-6000186 Msa1137280:intron 45.0%
AAGGAAAATGGTGAGCCTCT+TGG + chr7_4:6000302-6000321 Msa1137280:three_prime_UTR 45.0%
AGGAAAATGGTGAGCCTCTT+GGG + chr7_4:6000303-6000322 Msa1137280:three_prime_UTR 45.0%
ACATCATAAACCACCCCAAG+AGG - chr7_4:6000320-6000339 None:intergenic 45.0%
TGATGTGCTATCGCGTATGT+TGG + chr7_4:6000334-6000353 Msa1137280:three_prime_UTR 45.0%
TAGATGCAGATCACTTGTGC+AGG + chr7_4:6000378-6000397 Msa1137280:three_prime_UTR 45.0%
ATTGGGTTTCTAACTCCGGC+TGG + chr7_4:5997264-5997283 Msa1137280:five_prime_UTR 50.0%
TTGGGTTTCTAACTCCGGCT+GGG + chr7_4:5997265-5997284 Msa1137280:five_prime_UTR 50.0%
AACTCAACACTTCTCCCAGC+CGG - chr7_4:5997282-5997301 None:intergenic 50.0%
TGAAACCCACAACACACTGC+TGG + chr7_4:5998095-5998114 Msa1137280:five_prime_UTR 50.0%
TGCTTCCAGCAGTGTGTTGT+GGG - chr7_4:5998103-5998122 None:intergenic 50.0%
!! ATGGGAACTGGAGGGCATTA+GGG + chr7_4:5998656-5998675 Msa1137280:CDS 50.0%
TACTGTTCGGGATGCACGAA+AGG + chr7_4:5998691-5998710 Msa1137280:CDS 50.0%
! TTCCTCAAAGTGCTAGCTGC+AGG + chr7_4:5999022-5999041 Msa1137280:CDS 50.0%
AACCTGCAGCTAGCACTTTG+AGG - chr7_4:5999027-5999046 None:intergenic 50.0%
! TCAAAGTGCTAGCTGCAGGT+TGG + chr7_4:5999026-5999045 Msa1137280:CDS 50.0%
! CAAAGTGCTAGCTGCAGGTT+GGG + chr7_4:5999027-5999046 Msa1137280:CDS 50.0%
! GCCGTTGTCTGATTCTGAAG+AGG + chr7_4:5999119-5999138 Msa1137280:CDS 50.0%
!! TTCTGAAGAGGCGCCTGAAT+TGG + chr7_4:5999131-5999150 Msa1137280:CDS 50.0%
GAGCTATGCATGGTTCCATG+TGG - chr7_4:5999496-5999515 None:intergenic 50.0%
GGAAAATGGTGAGCCTCTTG+GGG + chr7_4:6000304-6000323 Msa1137280:three_prime_UTR 50.0%
GTGCAACGTCAAACGGCATG+TGG - chr7_4:5997202-5997221 None:intergenic 55.0%
CTGCTTCCAGCAGTGTGTTG+TGG - chr7_4:5998104-5998123 None:intergenic 55.0%
TCCAGTTCCCATCAACGACG+TGG - chr7_4:5998648-5998667 None:intergenic 55.0%
! ACGTCGTTGATGGGAACTGG+AGG + chr7_4:5998647-5998666 Msa1137280:CDS 55.0%
! CGTCGTTGATGGGAACTGGA+GGG + chr7_4:5998648-5998667 Msa1137280:CDS 55.0%
!! GATGGGAACTGGAGGGCATT+AGG + chr7_4:5998655-5998674 Msa1137280:CDS 55.0%
TGTTCGGGATGCACGAAAGG+TGG + chr7_4:5998694-5998713 Msa1137280:CDS 55.0%
AAATGGTGAGCCTCTTGGGG+TGG + chr7_4:6000307-6000326 Msa1137280:three_prime_UTR 55.0%
ACTGGGAGCCACGTCGTTGA+TGG + chr7_4:5998637-5998656 Msa1137280:CDS 60.0%
CTGGGAGCCACGTCGTTGAT+GGG + chr7_4:5998638-5998657 Msa1137280:CDS 60.0%
! GCCACGTCGTTGATGGGAAC+TGG + chr7_4:5998644-5998663 Msa1137280:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 5996409 6000788 5996409 ID=Msa1137280;Name=Msa1137280
chr7_4 mRNA 5996409 6000788 5996409 ID=Msa1137280-mRNA-1;Parent=Msa1137280;Name=Msa1137280-mRNA-1;_AED=0.03;_eAED=0.03;_QI=1467|1|1|1|0|0|3|546|250
chr7_4 exon 5996409 5996587 5996409 ID=Msa1137280-mRNA-1:exon:1656;Parent=Msa1137280-mRNA-1
chr7_4 exon 5997118 5999176 5997118 ID=Msa1137280-mRNA-1:exon:1657;Parent=Msa1137280-mRNA-1
chr7_4 exon 6000261 6000788 6000261 ID=Msa1137280-mRNA-1:exon:1658;Parent=Msa1137280-mRNA-1
chr7_4 five_prime_UTR 5996409 5996587 5996409 ID=Msa1137280-mRNA-1:five_prime_utr;Parent=Msa1137280-mRNA-1
chr7_4 five_prime_UTR 5997118 5998405 5997118 ID=Msa1137280-mRNA-1:five_prime_utr;Parent=Msa1137280-mRNA-1
chr7_4 CDS 5998406 5999158 5998406 ID=Msa1137280-mRNA-1:cds;Parent=Msa1137280-mRNA-1
chr7_4 three_prime_UTR 5999159 5999176 5999159 ID=Msa1137280-mRNA-1:three_prime_utr;Parent=Msa1137280-mRNA-1
chr7_4 three_prime_UTR 6000261 6000788 6000261 ID=Msa1137280-mRNA-1:three_prime_utr;Parent=Msa1137280-mRNA-1
Gene Sequence

>Msa1137280

ATGATTATCACAAAGCAGTACCGATGCATACATTCAGCTAGCTGTCAATGTTCCAAAGGGCATCTAAGTGAAGATGCGATTTTCTTAGTGTTTCATAATTTAAAATGGAATCCTAAGCTGATTGCTGCTCTATCATGTGTGTGCAAATGGTTTGACGATCTTGCCAAGCGAGTTCTATGGAAAGAGTTTTGTCGAACAAGAGCTCCAAAGATGTTGTGTGATCTGCAATCAACTGGGAGCCACGTCGTTGATGGGAACTGGAGGGCATTAGGGAAGCTTCTTACGTACTGTTCGGGATGCACGAAAGGTGGCTTGTTTAAAAACATTCAGAATCAGATCCCTGGTCACTTTGTGTATCAGACTCGATTTTCTAGAACATCAGGGAAGAGCTTTCTTTTGCCACAATGCAGAACTGATGTTTTGTATGTATCTGATCCTTGTGAGCATCTAGATCAAGGTGAAGAAGGAGATATTGGATTCTTCCGCGGAGTTTTTAAGTCGTTTGCAACGTCAAAGGTCAGAAAGATGCTTATTAGTAAAGGTGCTAAGCTTCATCAAACAGAAGTTTGCCCTTATTGTAAAGCGAAGTTGTGGAGCATGCAGCAAGCTAAAATGATTCCTCAAAGTGCTAGCTGCAGGTTGGGTTCTTATGAAGATGATATCGAGTATTTTGTGTGCCTCAATGGTCACTTGATTGGGATATGTACCCTATTGCCGTTGTCTGATTCTGAAGAGGCGCCTGAATTGGAGTAA
Protein sequence

>Msa1137280

MIITKQYRCIHSASCQCSKGHLSEDAIFLVFHNLKWNPKLIAALSCVCKWFDDLAKRVLWKEFCRTRAPKMLCDLQSTGSHVVDGNWRALGKLLTYCSGCTKGGLFKNIQNQIPGHFVYQTRFSRTSGKSFLLPQCRTDVLYVSDPCEHLDQGEEGDIGFFRGVFKSFATSKVRKMLISKGAKLHQTEVCPYCKAKLWSMQQAKMIPQSASCRLGSYEDDIEYFVCLNGHLIGICTLLPLSDSEEAPELE*