AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1028490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 89 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAGCATTATTTGCAAAA+TGG 0.185671 7_1:-61423191 None:intergenic
AATGGACGTTATTAATTTAT+AGG 0.194375 7_1:+61422466 Msa1028490:five_prime_UTR
ATTAATTAATATAGGGTAAT+AGG 0.249229 7_1:+61422495 Msa1028490:five_prime_UTR
TGAACCGTTGTGGGGTTAAT+AGG 0.250016 7_1:-61422598 None:intergenic
TCTCTTCCTTAATCTCCTTT+TGG 0.272655 7_1:-61423140 None:intergenic
ATTAAGGAAGAGAATGAAAA+TGG 0.290274 7_1:+61423150 Msa1028490:three_prime_UTR
TAGAGACCAAAAGGAGATTA+AGG 0.298692 7_1:+61423134 Msa1028490:three_prime_UTR
ATAGATTAGTATTATTATAA+TGG 0.302876 7_1:+61422448 Msa1028490:five_prime_UTR
ATCAGCATTATTTGCAAAAT+GGG 0.322522 7_1:-61423190 None:intergenic
GGTACATAATTAATTAATAT+AGG 0.347853 7_1:+61422487 Msa1028490:five_prime_UTR
TTCTACACCACCACCGATTC+CGG 0.355167 7_1:-61422828 None:intergenic
GTTCTTAATTGTGATATCAA+TGG 0.400941 7_1:-61422408 None:intergenic
GTACATAATTAATTAATATA+GGG 0.405835 7_1:+61422488 Msa1028490:five_prime_UTR
AACCACAAGCAATTCATTGT+TGG 0.420562 7_1:-61423223 None:intergenic
GGAATCGGTGGTGGTGTAGA+AGG 0.421946 7_1:+61422830 Msa1028490:CDS
GGAGGTGTAGTAGGAATTGA+TGG 0.433145 7_1:+61422851 Msa1028490:CDS
TATTTGCAAAATGGGTTCAA+AGG 0.444542 7_1:-61423182 None:intergenic
TGCACTCAGAGCTAATATCT+TGG 0.446855 7_1:-61422660 None:intergenic
ATTTGCAAAATGGGTTCAAA+GGG 0.456799 7_1:-61423181 None:intergenic
GGTGTAGAAGGAGGTGTAGT+AGG 0.462417 7_1:+61422842 Msa1028490:CDS
GGTGGTGGTGTGGAAGGAGG+TGG 0.463637 7_1:+61422992 Msa1028490:intron
AGACATGGTGGTGGTGGTAG+TGG 0.464802 7_1:+61423079 Msa1028490:CDS
AATACTAATCTATATATTTG+TGG 0.465925 7_1:-61422438 None:intergenic
GACGAAGGTGGAGGTGTGGA+AGG 0.467449 7_1:+61422890 Msa1028490:CDS
CAACAAGAGTAGAGACCAAA+AGG 0.469234 7_1:+61423125 Msa1028490:three_prime_UTR
GAATAATCTTGAACCGTTGT+GGG 0.474149 7_1:-61422607 None:intergenic
ATTGATGGTGGCGTGGAAGG+AGG 0.476846 7_1:+61422866 Msa1028490:CDS
GCACTCAGAGCTAATATCTT+GGG 0.477872 7_1:-61422659 None:intergenic
GTCGAAGGTGGTGGTGTGGA+AGG 0.478654 7_1:+61422986 Msa1028490:intron
GGAATTGATGGTGGCGTGGA+AGG 0.480177 7_1:+61422863 Msa1028490:CDS
AGGGGACGAAGGTGGAGGTG+TGG 0.485226 7_1:+61422886 Msa1028490:CDS
GGTGTCACCGGAATCGGTGG+TGG 0.488853 7_1:+61422821 Msa1028490:CDS
GTGGAAGGAGGGGACGAAGG+TGG 0.493083 7_1:+61422878 Msa1028490:CDS
ACCACAGGTGGTGGTGTTGA+AGG 0.497195 7_1:+61423028 Msa1028490:CDS
ATAGGTGGTGATGGAGAAGG+AGG 0.497300 7_1:+61422920 Msa1028490:CDS
TGATGGTGGCGTGGAAGGAG+GGG 0.497819 7_1:+61422868 Msa1028490:CDS
GATGGAGAAGGAGGGGTCGA+AGG 0.503510 7_1:+61422929 Msa1028490:CDS
GAAGGTGGTGGTGTGGAAGG+AGG 0.510965 7_1:+61422989 Msa1028490:intron
TTGATGGTGGCGTGGAAGGA+GGG 0.512687 7_1:+61422867 Msa1028490:CDS
TCTTAACATCAATGCTGACT+TGG 0.513922 7_1:-61422630 None:intergenic
TACAACAAGGATTGTTTCGT+GGG 0.515759 7_1:+61422732 Msa1028490:CDS
GAAGGAGGGGACGAAGGTGG+AGG 0.516524 7_1:+61422881 Msa1028490:CDS
GGAATAATCTTGAACCGTTG+TGG 0.519169 7_1:-61422608 None:intergenic
GGAGTTATCATAGGTGGTGA+TGG 0.522356 7_1:+61422911 Msa1028490:CDS
ATCATAGGTGGTGATGGAGA+AGG 0.523002 7_1:+61422917 Msa1028490:CDS
GATCAACAAGGCCGCTGATG+AGG 0.531095 7_1:+61422708 Msa1028490:CDS
TGTGGGGTTAATAGGCCCGC+TGG 0.535503 7_1:-61422590 None:intergenic
GTGGAAGGAGGTGGTGTCGA+AGG 0.538378 7_1:+61423001 Msa1028490:CDS
ATCAACAGGTGGTGGTGTAG+TGG 0.543768 7_1:+61423054 Msa1028490:CDS
CTAATATTCAACAAATTGAG+AGG 0.545252 7_1:+61422382 None:intergenic
GTGGAAAGACATGGTGGTGG+TGG 0.546531 7_1:+61423073 Msa1028490:CDS
CCATTGGCAATGGCATGCAT+TGG 0.559911 7_1:+61422524 Msa1028490:five_prime_UTR
TGGTGTCGAAGGTGGTGGTG+TGG 0.563486 7_1:+61422982 Msa1028490:intron
CTTAACATCAATGCTGACTT+GGG 0.569880 7_1:-61422629 None:intergenic
ATCAACAAGGCCGCTGATGA+GGG 0.570640 7_1:+61422709 Msa1028490:CDS
GGTGTGGAAGGAGTTATCAT+AGG 0.571036 7_1:+61422902 Msa1028490:CDS
CGTCTACATGTGAAACCAGC+GGG 0.571587 7_1:+61422575 Msa1028490:five_prime_UTR
GGTGTTGAAGGAGTATCAAC+AGG 0.573364 7_1:+61423040 Msa1028490:CDS
AAAATGGGTTCAAAGGGTGT+TGG 0.576868 7_1:-61423175 None:intergenic
CCAATGCATGCCATTGCCAA+TGG 0.578740 7_1:-61422524 None:intergenic
GTACAACAAGGATTGTTTCG+TGG 0.583674 7_1:+61422731 Msa1028490:CDS
AAGATATCATGTATGAGCAA+TGG 0.587650 7_1:-61422547 None:intergenic
GGCGTGGAAGGAGGGGACGA+AGG 0.588177 7_1:+61422875 Msa1028490:CDS
GGGTAATAGGAACCGTCCAT+TGG 0.590809 7_1:+61422508 Msa1028490:five_prime_UTR
GAAGGAGTTACCACAGGTGG+TGG 0.599734 7_1:+61423019 Msa1028490:CDS
TAGGAACCGTCCATTGGCAA+TGG 0.601035 7_1:+61422514 Msa1028490:five_prime_UTR
TAGGTGGTGATGGAGAAGGA+GGG 0.604228 7_1:+61422921 Msa1028490:CDS
TGCATGCCATTGCCAATGGA+CGG 0.604279 7_1:-61422520 None:intergenic
TCCTTCAACACCACCACCTG+TGG 0.606270 7_1:-61423029 None:intergenic
TAATATTCAACAAATTGAGA+GGG 0.610035 7_1:+61422383 None:intergenic
GTAGTGGAAAGACATGGTGG+TGG 0.621100 7_1:+61423070 Msa1028490:CDS
AATAATCTTGAACCGTTGTG+GGG 0.623368 7_1:-61422606 None:intergenic
CCTTGTTGTACCCTCATCAG+CGG 0.634928 7_1:-61422719 None:intergenic
GTGGAAGGAGTTATCATAGG+TGG 0.636745 7_1:+61422905 Msa1028490:CDS
GGTGTCGAAGGAGTTACCAC+AGG 0.637015 7_1:+61423013 Msa1028490:CDS
ATCGGTGGTGGTGTAGAAGG+AGG 0.638392 7_1:+61422833 Msa1028490:CDS
GAAGGAGTATCAACAGGTGG+TGG 0.647566 7_1:+61423046 Msa1028490:CDS
AGTAGGAATTGATGGTGGCG+TGG 0.650057 7_1:+61422859 Msa1028490:CDS
GGTGTAGTGGAAAGACATGG+TGG 0.654628 7_1:+61423067 Msa1028490:CDS
CCGCTGATGAGGGTACAACA+AGG 0.658102 7_1:+61422719 Msa1028490:CDS
GTCGAAGGAGTTACCACAGG+TGG 0.663904 7_1:+61423016 Msa1028490:CDS
ACGTCTACATGTGAAACCAG+CGG 0.672566 7_1:+61422574 Msa1028490:five_prime_UTR
AGGTGGTGATGGAGAAGGAG+GGG 0.673738 7_1:+61422922 Msa1028490:CDS
CAGAGGCATCTAGATCAACA+AGG 0.676803 7_1:+61422696 Msa1028490:CDS
GGTGTAGTAGGAATTGATGG+TGG 0.683408 7_1:+61422854 Msa1028490:CDS
GGTGGTGTAGTGGAAAGACA+TGG 0.694498 7_1:+61423064 Msa1028490:CDS
GTTGAAGGAGTATCAACAGG+TGG 0.747259 7_1:+61423043 Msa1028490:CDS
CGGGCCTATTAACCCCACAA+CGG 0.752886 7_1:+61422594 Msa1028490:five_prime_UTR
TGCAGCGCAAAGACACACAG+AGG 0.757164 7_1:+61422679 Msa1028490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGATTAGTATTATTATAA+TGG + chr7_1:61422448-61422467 Msa1028490:five_prime_UTR 10.0%
!! GTACATAATTAATTAATATA+GGG + chr7_1:61422488-61422507 Msa1028490:five_prime_UTR 10.0%
!! ATAGATTAGTATTATTATAA+TGG + chr7_1:61422448-61422467 Msa1028490:five_prime_UTR 10.0%
!! GTACATAATTAATTAATATA+GGG + chr7_1:61422488-61422507 Msa1028490:five_prime_UTR 10.0%
!! AATACTAATCTATATATTTG+TGG - chr7_1:61422441-61422460 None:intergenic 15.0%
!! GGTACATAATTAATTAATAT+AGG + chr7_1:61422487-61422506 Msa1028490:five_prime_UTR 15.0%
!! ATTAATTAATATAGGGTAAT+AGG + chr7_1:61422495-61422514 Msa1028490:five_prime_UTR 15.0%
!! AATACTAATCTATATATTTG+TGG - chr7_1:61422441-61422460 None:intergenic 15.0%
!! GGTACATAATTAATTAATAT+AGG + chr7_1:61422487-61422506 Msa1028490:five_prime_UTR 15.0%
!! ATTAATTAATATAGGGTAAT+AGG + chr7_1:61422495-61422514 Msa1028490:five_prime_UTR 15.0%
!! AATGGACGTTATTAATTTAT+AGG + chr7_1:61422466-61422485 Msa1028490:five_prime_UTR 20.0%
!!! TTTAAAAGGTTACAATTTTG+CGG + chr7_1:61422797-61422816 Msa1028490:CDS 20.0%
!! AATGGACGTTATTAATTTAT+AGG + chr7_1:61422466-61422485 Msa1028490:five_prime_UTR 20.0%
!!! TTTAAAAGGTTACAATTTTG+CGG + chr7_1:61422797-61422816 Msa1028490:CDS 20.0%
! GTTCTTAATTGTGATATCAA+TGG - chr7_1:61422411-61422430 None:intergenic 25.0%
!!! CGCAAATTTTCAAGTTTAAA+AGG + chr7_1:61422783-61422802 Msa1028490:CDS 25.0%
!! TTAAAAGGTTACAATTTTGC+GGG + chr7_1:61422798-61422817 Msa1028490:CDS 25.0%
! ATTAAGGAAGAGAATGAAAA+TGG + chr7_1:61423150-61423169 Msa1028490:three_prime_UTR 25.0%
! ATCAGCATTATTTGCAAAAT+GGG - chr7_1:61423193-61423212 None:intergenic 25.0%
! AATCAGCATTATTTGCAAAA+TGG - chr7_1:61423194-61423213 None:intergenic 25.0%
! GTTCTTAATTGTGATATCAA+TGG - chr7_1:61422411-61422430 None:intergenic 25.0%
!!! CGCAAATTTTCAAGTTTAAA+AGG + chr7_1:61422783-61422802 Msa1028490:CDS 25.0%
!! TTAAAAGGTTACAATTTTGC+GGG + chr7_1:61422798-61422817 Msa1028490:CDS 25.0%
! ATTAAGGAAGAGAATGAAAA+TGG + chr7_1:61423150-61423169 Msa1028490:three_prime_UTR 25.0%
! ATCAGCATTATTTGCAAAAT+GGG - chr7_1:61423193-61423212 None:intergenic 25.0%
! AATCAGCATTATTTGCAAAA+TGG - chr7_1:61423194-61423213 None:intergenic 25.0%
AAGATATCATGTATGAGCAA+TGG - chr7_1:61422550-61422569 None:intergenic 30.0%
! TAAAAGGTTACAATTTTGCG+GGG + chr7_1:61422799-61422818 Msa1028490:CDS 30.0%
! ATTTGCAAAATGGGTTCAAA+GGG - chr7_1:61423184-61423203 None:intergenic 30.0%
! TATTTGCAAAATGGGTTCAA+AGG - chr7_1:61423185-61423204 None:intergenic 30.0%
AAGATATCATGTATGAGCAA+TGG - chr7_1:61422550-61422569 None:intergenic 30.0%
! TAAAAGGTTACAATTTTGCG+GGG + chr7_1:61422799-61422818 Msa1028490:CDS 30.0%
! ATTTGCAAAATGGGTTCAAA+GGG - chr7_1:61423184-61423203 None:intergenic 30.0%
! TATTTGCAAAATGGGTTCAA+AGG - chr7_1:61423185-61423204 None:intergenic 30.0%
AATAATCTTGAACCGTTGTG+GGG - chr7_1:61422609-61422628 None:intergenic 35.0%
GAATAATCTTGAACCGTTGT+GGG - chr7_1:61422610-61422629 None:intergenic 35.0%
!! CTTAACATCAATGCTGACTT+GGG - chr7_1:61422632-61422651 None:intergenic 35.0%
! TCTTAACATCAATGCTGACT+TGG - chr7_1:61422633-61422652 None:intergenic 35.0%
TACAACAAGGATTGTTTCGT+GGG + chr7_1:61422732-61422751 Msa1028490:CDS 35.0%
! AAAAGGTTACAATTTTGCGG+GGG + chr7_1:61422800-61422819 Msa1028490:CDS 35.0%
TAGAGACCAAAAGGAGATTA+AGG + chr7_1:61423134-61423153 Msa1028490:three_prime_UTR 35.0%
! TCTCTTCCTTAATCTCCTTT+TGG - chr7_1:61423143-61423162 None:intergenic 35.0%
AATAATCTTGAACCGTTGTG+GGG - chr7_1:61422609-61422628 None:intergenic 35.0%
GAATAATCTTGAACCGTTGT+GGG - chr7_1:61422610-61422629 None:intergenic 35.0%
!! CTTAACATCAATGCTGACTT+GGG - chr7_1:61422632-61422651 None:intergenic 35.0%
! TCTTAACATCAATGCTGACT+TGG - chr7_1:61422633-61422652 None:intergenic 35.0%
TACAACAAGGATTGTTTCGT+GGG + chr7_1:61422732-61422751 Msa1028490:CDS 35.0%
! AAAAGGTTACAATTTTGCGG+GGG + chr7_1:61422800-61422819 Msa1028490:CDS 35.0%
TAGAGACCAAAAGGAGATTA+AGG + chr7_1:61423134-61423153 Msa1028490:three_prime_UTR 35.0%
! TCTCTTCCTTAATCTCCTTT+TGG - chr7_1:61423143-61423162 None:intergenic 35.0%
GGAATAATCTTGAACCGTTG+TGG - chr7_1:61422611-61422630 None:intergenic 40.0%
GCACTCAGAGCTAATATCTT+GGG - chr7_1:61422662-61422681 None:intergenic 40.0%
TGCACTCAGAGCTAATATCT+TGG - chr7_1:61422663-61422682 None:intergenic 40.0%
GTACAACAAGGATTGTTTCG+TGG + chr7_1:61422731-61422750 Msa1028490:CDS 40.0%
CAACAAGAGTAGAGACCAAA+AGG + chr7_1:61423125-61423144 Msa1028490:three_prime_UTR 40.0%
! AAAATGGGTTCAAAGGGTGT+TGG - chr7_1:61423178-61423197 None:intergenic 40.0%
GGAATAATCTTGAACCGTTG+TGG - chr7_1:61422611-61422630 None:intergenic 40.0%
GCACTCAGAGCTAATATCTT+GGG - chr7_1:61422662-61422681 None:intergenic 40.0%
TGCACTCAGAGCTAATATCT+TGG - chr7_1:61422663-61422682 None:intergenic 40.0%
GTACAACAAGGATTGTTTCG+TGG + chr7_1:61422731-61422750 Msa1028490:CDS 40.0%
CAACAAGAGTAGAGACCAAA+AGG + chr7_1:61423125-61423144 Msa1028490:three_prime_UTR 40.0%
! AAAATGGGTTCAAAGGGTGT+TGG - chr7_1:61423178-61423197 None:intergenic 40.0%
ACGTCTACATGTGAAACCAG+CGG + chr7_1:61422574-61422593 Msa1028490:five_prime_UTR 45.0%
TGAACCGTTGTGGGGTTAAT+AGG - chr7_1:61422601-61422620 None:intergenic 45.0%
!! CAGAGGCATCTAGATCAACA+AGG + chr7_1:61422696-61422715 Msa1028490:CDS 45.0%
! GGAGGTGTAGTAGGAATTGA+TGG + chr7_1:61422851-61422870 Msa1028490:CDS 45.0%
GGTGTAGTAGGAATTGATGG+TGG + chr7_1:61422854-61422873 Msa1028490:CDS 45.0%
GGTGTGGAAGGAGTTATCAT+AGG + chr7_1:61422902-61422921 Msa1028490:CDS 45.0%
GTGGAAGGAGTTATCATAGG+TGG + chr7_1:61422905-61422924 Msa1028490:CDS 45.0%
GGAGTTATCATAGGTGGTGA+TGG + chr7_1:61422911-61422930 Msa1028490:CDS 45.0%
!! ATCATAGGTGGTGATGGAGA+AGG + chr7_1:61422917-61422936 Msa1028490:CDS 45.0%
!! TGGTGTTGATGGATCAACCA+CGG + chr7_1:61422955-61422974 Msa1028490:intron 45.0%
!! GGTGTTGAAGGAGTATCAAC+AGG + chr7_1:61423040-61423059 Msa1028490:CDS 45.0%
!! GTTGAAGGAGTATCAACAGG+TGG + chr7_1:61423043-61423062 Msa1028490:CDS 45.0%
ACGTCTACATGTGAAACCAG+CGG + chr7_1:61422574-61422593 Msa1028490:five_prime_UTR 45.0%
TGAACCGTTGTGGGGTTAAT+AGG - chr7_1:61422601-61422620 None:intergenic 45.0%
!! CAGAGGCATCTAGATCAACA+AGG + chr7_1:61422696-61422715 Msa1028490:CDS 45.0%
! GGAGGTGTAGTAGGAATTGA+TGG + chr7_1:61422851-61422870 Msa1028490:CDS 45.0%
GGTGTAGTAGGAATTGATGG+TGG + chr7_1:61422854-61422873 Msa1028490:CDS 45.0%
GGTGTGGAAGGAGTTATCAT+AGG + chr7_1:61422902-61422921 Msa1028490:CDS 45.0%
GTGGAAGGAGTTATCATAGG+TGG + chr7_1:61422905-61422924 Msa1028490:CDS 45.0%
GGAGTTATCATAGGTGGTGA+TGG + chr7_1:61422911-61422930 Msa1028490:CDS 45.0%
!! ATCATAGGTGGTGATGGAGA+AGG + chr7_1:61422917-61422936 Msa1028490:CDS 45.0%
!! TGGTGTTGATGGATCAACCA+CGG + chr7_1:61422955-61422974 Msa1028490:intron 45.0%
!! GGTGTTGAAGGAGTATCAAC+AGG + chr7_1:61423040-61423059 Msa1028490:CDS 45.0%
!! GTTGAAGGAGTATCAACAGG+TGG + chr7_1:61423043-61423062 Msa1028490:CDS 45.0%
GGGTAATAGGAACCGTCCAT+TGG + chr7_1:61422508-61422527 Msa1028490:five_prime_UTR 50.0%
TAGGAACCGTCCATTGGCAA+TGG + chr7_1:61422514-61422533 Msa1028490:five_prime_UTR 50.0%
TGCATGCCATTGCCAATGGA+CGG - chr7_1:61422523-61422542 None:intergenic 50.0%
CCAATGCATGCCATTGCCAA+TGG - chr7_1:61422527-61422546 None:intergenic 50.0%
CCATTGGCAATGGCATGCAT+TGG + chr7_1:61422524-61422543 Msa1028490:five_prime_UTR 50.0%
CGTCTACATGTGAAACCAGC+GGG + chr7_1:61422575-61422594 Msa1028490:five_prime_UTR 50.0%
ATCAACAAGGCCGCTGATGA+GGG + chr7_1:61422709-61422728 Msa1028490:CDS 50.0%
! CCTTGTTGTACCCTCATCAG+CGG - chr7_1:61422722-61422741 None:intergenic 50.0%
!! TTCTACACCACCACCGATTC+CGG - chr7_1:61422831-61422850 None:intergenic 50.0%
! GGTGTAGAAGGAGGTGTAGT+AGG + chr7_1:61422842-61422861 Msa1028490:CDS 50.0%
!! AGTAGGAATTGATGGTGGCG+TGG + chr7_1:61422859-61422878 Msa1028490:CDS 50.0%
!! ATAGGTGGTGATGGAGAAGG+AGG + chr7_1:61422920-61422939 Msa1028490:CDS 50.0%
!! TAGGTGGTGATGGAGAAGGA+GGG + chr7_1:61422921-61422940 Msa1028490:CDS 50.0%
!! GGTGTTGATGGATCAACCAC+GGG + chr7_1:61422956-61422975 Msa1028490:intron 50.0%
!! GAAGGAGTATCAACAGGTGG+TGG + chr7_1:61423046-61423065 Msa1028490:CDS 50.0%
! ATCAACAGGTGGTGGTGTAG+TGG + chr7_1:61423054-61423073 Msa1028490:CDS 50.0%
! GGTGGTGTAGTGGAAAGACA+TGG + chr7_1:61423064-61423083 Msa1028490:CDS 50.0%
! GGTGTAGTGGAAAGACATGG+TGG + chr7_1:61423067-61423086 Msa1028490:CDS 50.0%
! GTAGTGGAAAGACATGGTGG+TGG + chr7_1:61423070-61423089 Msa1028490:CDS 50.0%
GGGTAATAGGAACCGTCCAT+TGG + chr7_1:61422508-61422527 Msa1028490:five_prime_UTR 50.0%
TAGGAACCGTCCATTGGCAA+TGG + chr7_1:61422514-61422533 Msa1028490:five_prime_UTR 50.0%
TGCATGCCATTGCCAATGGA+CGG - chr7_1:61422523-61422542 None:intergenic 50.0%
CCAATGCATGCCATTGCCAA+TGG - chr7_1:61422527-61422546 None:intergenic 50.0%
CCATTGGCAATGGCATGCAT+TGG + chr7_1:61422524-61422543 Msa1028490:five_prime_UTR 50.0%
CGTCTACATGTGAAACCAGC+GGG + chr7_1:61422575-61422594 Msa1028490:five_prime_UTR 50.0%
ATCAACAAGGCCGCTGATGA+GGG + chr7_1:61422709-61422728 Msa1028490:CDS 50.0%
! CCTTGTTGTACCCTCATCAG+CGG - chr7_1:61422722-61422741 None:intergenic 50.0%
!! TTCTACACCACCACCGATTC+CGG - chr7_1:61422831-61422850 None:intergenic 50.0%
! GGTGTAGAAGGAGGTGTAGT+AGG + chr7_1:61422842-61422861 Msa1028490:CDS 50.0%
!! AGTAGGAATTGATGGTGGCG+TGG + chr7_1:61422859-61422878 Msa1028490:CDS 50.0%
!! ATAGGTGGTGATGGAGAAGG+AGG + chr7_1:61422920-61422939 Msa1028490:CDS 50.0%
!! TAGGTGGTGATGGAGAAGGA+GGG + chr7_1:61422921-61422940 Msa1028490:CDS 50.0%
!! GGTGTTGATGGATCAACCAC+GGG + chr7_1:61422956-61422975 Msa1028490:intron 50.0%
!! GAAGGAGTATCAACAGGTGG+TGG + chr7_1:61423046-61423065 Msa1028490:CDS 50.0%
! ATCAACAGGTGGTGGTGTAG+TGG + chr7_1:61423054-61423073 Msa1028490:CDS 50.0%
! GGTGGTGTAGTGGAAAGACA+TGG + chr7_1:61423064-61423083 Msa1028490:CDS 50.0%
! GGTGTAGTGGAAAGACATGG+TGG + chr7_1:61423067-61423086 Msa1028490:CDS 50.0%
! GTAGTGGAAAGACATGGTGG+TGG + chr7_1:61423070-61423089 Msa1028490:CDS 50.0%
CGGGCCTATTAACCCCACAA+CGG + chr7_1:61422594-61422613 Msa1028490:five_prime_UTR 55.0%
TGCAGCGCAAAGACACACAG+AGG + chr7_1:61422679-61422698 Msa1028490:CDS 55.0%
GATCAACAAGGCCGCTGATG+AGG + chr7_1:61422708-61422727 Msa1028490:CDS 55.0%
! CCGCTGATGAGGGTACAACA+AGG + chr7_1:61422719-61422738 Msa1028490:CDS 55.0%
!!! CAATTTTGCGGGGGTGTCAC+CGG + chr7_1:61422809-61422828 Msa1028490:CDS 55.0%
! GGAATCGGTGGTGGTGTAGA+AGG + chr7_1:61422830-61422849 Msa1028490:CDS 55.0%
! ATCGGTGGTGGTGTAGAAGG+AGG + chr7_1:61422833-61422852 Msa1028490:CDS 55.0%
!! GGAATTGATGGTGGCGTGGA+AGG + chr7_1:61422863-61422882 Msa1028490:CDS 55.0%
!! ATTGATGGTGGCGTGGAAGG+AGG + chr7_1:61422866-61422885 Msa1028490:CDS 55.0%
!! TTGATGGTGGCGTGGAAGGA+GGG + chr7_1:61422867-61422886 Msa1028490:CDS 55.0%
!! AGGTGGTGATGGAGAAGGAG+GGG + chr7_1:61422922-61422941 Msa1028490:CDS 55.0%
! GTCGAAGGTGGTGGTGTTGA+TGG + chr7_1:61422944-61422963 Msa1028490:intron 55.0%
! GTTGATGGATCAACCACGGG+TGG + chr7_1:61422959-61422978 Msa1028490:intron 55.0%
! GGTGTCGAAGGAGTTACCAC+AGG + chr7_1:61423013-61423032 Msa1028490:CDS 55.0%
GTCGAAGGAGTTACCACAGG+TGG + chr7_1:61423016-61423035 Msa1028490:CDS 55.0%
GAAGGAGTTACCACAGGTGG+TGG + chr7_1:61423019-61423038 Msa1028490:CDS 55.0%
! ACCACAGGTGGTGGTGTTGA+AGG + chr7_1:61423028-61423047 Msa1028490:CDS 55.0%
TCCTTCAACACCACCACCTG+TGG - chr7_1:61423032-61423051 None:intergenic 55.0%
!! GTGGAAAGACATGGTGGTGG+TGG + chr7_1:61423073-61423092 Msa1028490:CDS 55.0%
!! AGACATGGTGGTGGTGGTAG+TGG + chr7_1:61423079-61423098 Msa1028490:CDS 55.0%
CGGGCCTATTAACCCCACAA+CGG + chr7_1:61422594-61422613 Msa1028490:five_prime_UTR 55.0%
TGCAGCGCAAAGACACACAG+AGG + chr7_1:61422679-61422698 Msa1028490:CDS 55.0%
GATCAACAAGGCCGCTGATG+AGG + chr7_1:61422708-61422727 Msa1028490:CDS 55.0%
! CCGCTGATGAGGGTACAACA+AGG + chr7_1:61422719-61422738 Msa1028490:CDS 55.0%
!!! CAATTTTGCGGGGGTGTCAC+CGG + chr7_1:61422809-61422828 Msa1028490:CDS 55.0%
! GGAATCGGTGGTGGTGTAGA+AGG + chr7_1:61422830-61422849 Msa1028490:CDS 55.0%
! ATCGGTGGTGGTGTAGAAGG+AGG + chr7_1:61422833-61422852 Msa1028490:CDS 55.0%
!! GGAATTGATGGTGGCGTGGA+AGG + chr7_1:61422863-61422882 Msa1028490:CDS 55.0%
!! ATTGATGGTGGCGTGGAAGG+AGG + chr7_1:61422866-61422885 Msa1028490:CDS 55.0%
!! TTGATGGTGGCGTGGAAGGA+GGG + chr7_1:61422867-61422886 Msa1028490:CDS 55.0%
!! AGGTGGTGATGGAGAAGGAG+GGG + chr7_1:61422922-61422941 Msa1028490:CDS 55.0%
! GTCGAAGGTGGTGGTGTTGA+TGG + chr7_1:61422944-61422963 Msa1028490:intron 55.0%
! GTTGATGGATCAACCACGGG+TGG + chr7_1:61422959-61422978 Msa1028490:intron 55.0%
! GGTGTCGAAGGAGTTACCAC+AGG + chr7_1:61423013-61423032 Msa1028490:CDS 55.0%
GTCGAAGGAGTTACCACAGG+TGG + chr7_1:61423016-61423035 Msa1028490:CDS 55.0%
GAAGGAGTTACCACAGGTGG+TGG + chr7_1:61423019-61423038 Msa1028490:CDS 55.0%
! ACCACAGGTGGTGGTGTTGA+AGG + chr7_1:61423028-61423047 Msa1028490:CDS 55.0%
TCCTTCAACACCACCACCTG+TGG - chr7_1:61423032-61423051 None:intergenic 55.0%
!! GTGGAAAGACATGGTGGTGG+TGG + chr7_1:61423073-61423092 Msa1028490:CDS 55.0%
!! AGACATGGTGGTGGTGGTAG+TGG + chr7_1:61423079-61423098 Msa1028490:CDS 55.0%
TGTGGGGTTAATAGGCCCGC+TGG - chr7_1:61422593-61422612 None:intergenic 60.0%
!! TGATGGTGGCGTGGAAGGAG+GGG + chr7_1:61422868-61422887 Msa1028490:CDS 60.0%
! GACGAAGGTGGAGGTGTGGA+AGG + chr7_1:61422890-61422909 Msa1028490:CDS 60.0%
GATGGAGAAGGAGGGGTCGA+AGG + chr7_1:61422929-61422948 Msa1028490:CDS 60.0%
GATGGATCAACCACGGGTGG+TGG + chr7_1:61422962-61422981 Msa1028490:intron 60.0%
! TGGTGTCGAAGGTGGTGGTG+TGG + chr7_1:61422982-61423001 Msa1028490:intron 60.0%
! GTCGAAGGTGGTGGTGTGGA+AGG + chr7_1:61422986-61423005 Msa1028490:intron 60.0%
! GAAGGTGGTGGTGTGGAAGG+AGG + chr7_1:61422989-61423008 Msa1028490:intron 60.0%
! GTGGAAGGAGGTGGTGTCGA+AGG + chr7_1:61423001-61423020 Msa1028490:CDS 60.0%
TGTGGGGTTAATAGGCCCGC+TGG - chr7_1:61422593-61422612 None:intergenic 60.0%
!! TGATGGTGGCGTGGAAGGAG+GGG + chr7_1:61422868-61422887 Msa1028490:CDS 60.0%
! GACGAAGGTGGAGGTGTGGA+AGG + chr7_1:61422890-61422909 Msa1028490:CDS 60.0%
GATGGAGAAGGAGGGGTCGA+AGG + chr7_1:61422929-61422948 Msa1028490:CDS 60.0%
GATGGATCAACCACGGGTGG+TGG + chr7_1:61422962-61422981 Msa1028490:intron 60.0%
! TGGTGTCGAAGGTGGTGGTG+TGG + chr7_1:61422982-61423001 Msa1028490:intron 60.0%
! GTCGAAGGTGGTGGTGTGGA+AGG + chr7_1:61422986-61423005 Msa1028490:intron 60.0%
! GAAGGTGGTGGTGTGGAAGG+AGG + chr7_1:61422989-61423008 Msa1028490:intron 60.0%
! GTGGAAGGAGGTGGTGTCGA+AGG + chr7_1:61423001-61423020 Msa1028490:CDS 60.0%
!! TGCGGGGGTGTCACCGGAAT+CGG + chr7_1:61422815-61422834 Msa1028490:CDS 65.0%
! GGTGTCACCGGAATCGGTGG+TGG + chr7_1:61422821-61422840 Msa1028490:CDS 65.0%
GTGGAAGGAGGGGACGAAGG+TGG + chr7_1:61422878-61422897 Msa1028490:CDS 65.0%
GAAGGAGGGGACGAAGGTGG+AGG + chr7_1:61422881-61422900 Msa1028490:CDS 65.0%
AGGGGACGAAGGTGGAGGTG+TGG + chr7_1:61422886-61422905 Msa1028490:CDS 65.0%
GGAGAAGGAGGGGTCGAAGG+TGG + chr7_1:61422932-61422951 Msa1028490:intron 65.0%
GAAGGAGGGGTCGAAGGTGG+TGG + chr7_1:61422935-61422954 Msa1028490:intron 65.0%
! ACCACGGGTGGTGGTGTCGA+AGG + chr7_1:61422971-61422990 Msa1028490:intron 65.0%
ACCTTCGACACCACCACCCG+TGG - chr7_1:61422975-61422994 None:intergenic 65.0%
! ACGGGTGGTGGTGTCGAAGG+TGG + chr7_1:61422974-61422993 Msa1028490:intron 65.0%
! GGTGGTGGTGTCGAAGGTGG+TGG + chr7_1:61422977-61422996 Msa1028490:intron 65.0%
! GGTGGTGGTGTGGAAGGAGG+TGG + chr7_1:61422992-61423011 Msa1028490:intron 65.0%
!! TGCGGGGGTGTCACCGGAAT+CGG + chr7_1:61422815-61422834 Msa1028490:CDS 65.0%
! GGTGTCACCGGAATCGGTGG+TGG + chr7_1:61422821-61422840 Msa1028490:CDS 65.0%
GTGGAAGGAGGGGACGAAGG+TGG + chr7_1:61422878-61422897 Msa1028490:CDS 65.0%
GAAGGAGGGGACGAAGGTGG+AGG + chr7_1:61422881-61422900 Msa1028490:CDS 65.0%
AGGGGACGAAGGTGGAGGTG+TGG + chr7_1:61422886-61422905 Msa1028490:CDS 65.0%
GGAGAAGGAGGGGTCGAAGG+TGG + chr7_1:61422932-61422951 Msa1028490:intron 65.0%
GAAGGAGGGGTCGAAGGTGG+TGG + chr7_1:61422935-61422954 Msa1028490:intron 65.0%
! ACCACGGGTGGTGGTGTCGA+AGG + chr7_1:61422971-61422990 Msa1028490:intron 65.0%
ACCTTCGACACCACCACCCG+TGG - chr7_1:61422975-61422994 None:intergenic 65.0%
! ACGGGTGGTGGTGTCGAAGG+TGG + chr7_1:61422974-61422993 Msa1028490:intron 65.0%
! GGTGGTGGTGTCGAAGGTGG+TGG + chr7_1:61422977-61422996 Msa1028490:intron 65.0%
! GGTGGTGGTGTGGAAGGAGG+TGG + chr7_1:61422992-61423011 Msa1028490:intron 65.0%
!! GGGGGTGTCACCGGAATCGG+TGG + chr7_1:61422818-61422837 Msa1028490:CDS 70.0%
GGCGTGGAAGGAGGGGACGA+AGG + chr7_1:61422875-61422894 Msa1028490:CDS 70.0%
!! GGGGGTGTCACCGGAATCGG+TGG + chr7_1:61422818-61422837 Msa1028490:CDS 70.0%
GGCGTGGAAGGAGGGGACGA+AGG + chr7_1:61422875-61422894 Msa1028490:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 61422395 61423237 61422395 ID=Msa1028490;Name=Msa1028490
chr7_1 mRNA 61422395 61423237 61422395 ID=Msa1028490-mRNA-1;Parent=Msa1028490;Name=Msa1028490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=249|1|1|1|0|0|2|120|143
chr7_1 exon 61422395 61422950 61422395 ID=Msa1028490-mRNA-1:exon:12933;Parent=Msa1028490-mRNA-1
chr7_1 exon 61422993 61423237 61422993 ID=Msa1028490-mRNA-1:exon:12934;Parent=Msa1028490-mRNA-1
chr7_1 five_prime_UTR 61422395 61422643 61422395 ID=Msa1028490-mRNA-1:five_prime_utr;Parent=Msa1028490-mRNA-1
chr7_1 CDS 61422644 61422950 61422644 ID=Msa1028490-mRNA-1:cds;Parent=Msa1028490-mRNA-1
chr7_1 CDS 61422993 61423117 61422993 ID=Msa1028490-mRNA-1:cds;Parent=Msa1028490-mRNA-1
chr7_1 three_prime_UTR 61423118 61423237 61423118 ID=Msa1028490-mRNA-1:three_prime_utr;Parent=Msa1028490-mRNA-1
Gene Sequence

>Msa1028490

ATGTTAAGACGTATTCCCAAGATATTAGCTCTGAGTGCAGCGCAAAGACACACAGAGGCATCTAGATCAACAAGGCCGCTGATGAGGGTACAACAAGGATTGTTTCGTGGGTTGAACACAACTGCATTCAATACGTTAGCGCAAATTTTCAAGTTTAAAAGGTTACAATTTTGCGGGGGTGTCACCGGAATCGGTGGTGGTGTAGAAGGAGGTGTAGTAGGAATTGATGGTGGCGTGGAAGGAGGGGACGAAGGTGGAGGTGTGGAAGGAGTTATCATAGGTGGTGATGGAGAAGGAGGGGTCGAAGGTGGTGGTGTGGAAGGAGGTGGTGTCGAAGGAGTTACCACAGGTGGTGGTGTTGAAGGAGTATCAACAGGTGGTGGTGTAGTGGAAAGACATGGTGGTGGTGGTAGTGGTCTCGTAAGAGCATGA
Protein sequence

>Msa1028490

MLRRIPKILALSAAQRHTEASRSTRPLMRVQQGLFRGLNTTAFNTLAQIFKFKRLQFCGGVTGIGGGVEGGVVGIDGGVEGGDEGGGVEGVIIGGDGEGGVEGGGVEGGGVEGVTTGGGVEGVSTGGGVVERHGGGGSGLVRA*