AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1112870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 90 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAGCATTATTTGCAAAA+TGG 0.185671 7_3:-58843284 None:intergenic
AATGGACGTTATTAATTTAT+AGG 0.194375 7_3:+58842562 Msa1112870:five_prime_UTR
ATTAATTAATATAGGGTAAT+AGG 0.249229 7_3:+58842591 Msa1112870:five_prime_UTR
TGAACCGTTGTGGGGTTAAT+AGG 0.250016 7_3:-58842694 None:intergenic
TCTCTTCCTTAATCTCCTTT+TGG 0.272655 7_3:-58843233 None:intergenic
ATTAAGGAAGAGAATGAAAA+TGG 0.290274 7_3:+58843243 Msa1112870:three_prime_UTR
TAGAGACCAAAAGGAGATTA+AGG 0.298692 7_3:+58843227 Msa1112870:three_prime_UTR
ATAGATTAGTATTATTATAA+TGG 0.302876 7_3:+58842544 Msa1112870:five_prime_UTR
ATCAGCATTATTTGCAAAAT+GGG 0.322522 7_3:-58843283 None:intergenic
GGTACATAATTAATTAATAT+AGG 0.347853 7_3:+58842583 Msa1112870:five_prime_UTR
GGTGTCACCGGAATCGGTGG+TGG 0.349829 7_3:+58842917 Msa1112870:CDS
TTCTACCCCACCACCGATTC+CGG 0.354378 7_3:-58842924 None:intergenic
GGAATCGGTGGTGGGGTAGA+AGG 0.364887 7_3:+58842926 Msa1112870:CDS
GTTCTTAATTGTGATATCAA+TGG 0.400941 7_3:-58842504 None:intergenic
GTACATAATTAATTAATATA+GGG 0.405835 7_3:+58842584 Msa1112870:five_prime_UTR
GGAGGTGTCGTAGGAATTGA+TGG 0.412771 7_3:+58842947 Msa1112870:CDS
TGTCACCGGAATCGGTGGTG+GGG 0.420222 7_3:+58842919 Msa1112870:CDS
AACCACAAGCAATTCATTGT+TGG 0.420562 7_3:-58843316 None:intergenic
TATTTGCAAAATGGGTTCAA+AGG 0.444542 7_3:-58843275 None:intergenic
TGCACTCAGAGCTAATATCT+TGG 0.446855 7_3:-58842756 None:intergenic
GTGTCACCGGAATCGGTGGT+GGG 0.451953 7_3:+58842918 Msa1112870:CDS
ATTTGCAAAATGGGTTCAAA+GGG 0.456799 7_3:-58843274 None:intergenic
GGTGGTGGTGTGGAAGGAGG+TGG 0.463637 7_3:+58843088 Msa1112870:intron
AGACATGGTGGTGGTGGTAG+TGG 0.464802 7_3:+58843172 Msa1112870:CDS
AATACTAATCTATATATTTG+TGG 0.465925 7_3:-58842534 None:intergenic
GACGAAGGTGGAGGTGTGGA+AGG 0.467449 7_3:+58842986 Msa1112870:CDS
CAACAAGAGTAGAGACCAAA+AGG 0.469234 7_3:+58843218 Msa1112870:three_prime_UTR
GAATAATCTTGAACCGTTGT+GGG 0.474149 7_3:-58842703 None:intergenic
ATTGATGGTGGCGTGGAAGG+AGG 0.476846 7_3:+58842962 Msa1112870:CDS
GCACTCAGAGCTAATATCTT+GGG 0.477872 7_3:-58842755 None:intergenic
GTCGAAGGTGGTGGTGTGGA+AGG 0.478654 7_3:+58843082 Msa1112870:intron
GGGGTAGAAGGAGGTGTCGT+AGG 0.478999 7_3:+58842938 Msa1112870:CDS
AGGGGACGAAGGTGGAGGTG+TGG 0.485226 7_3:+58842982 Msa1112870:CDS
GTGGAAAGACATGGTGGTGG+TGG 0.485554 7_3:+58843166 Msa1112870:CDS
GTGGAAGGAGGGGACGAAGG+TGG 0.493083 7_3:+58842974 Msa1112870:CDS
ACCACAGGTGGTGGTGTTGA+AGG 0.497195 7_3:+58843124 Msa1112870:CDS
TACAGCAAGGATTGTTTCGT+GGG 0.497415 7_3:+58842828 Msa1112870:CDS
TGATGGTGGCGTGGAAGGAG+GGG 0.497819 7_3:+58842964 Msa1112870:CDS
GAAGGTGGTGGTGTGGAAGG+AGG 0.510965 7_3:+58843085 Msa1112870:intron
TTGATGGTGGCGTGGAAGGA+GGG 0.512687 7_3:+58842963 Msa1112870:CDS
TCTTAACATCAATGCTGACT+TGG 0.513922 7_3:-58842726 None:intergenic
GAAGGAGGGGACGAAGGTGG+AGG 0.516524 7_3:+58842977 Msa1112870:CDS
GGAATTGATGGTGGCGTGGA+AGG 0.518694 7_3:+58842959 Msa1112870:CDS
GGAATAATCTTGAACCGTTG+TGG 0.519169 7_3:-58842704 None:intergenic
GGAGTTATCATAGGTGGTGA+TGG 0.522356 7_3:+58843007 Msa1112870:CDS
ATCATAGGTGGTGATGGAGA+AGG 0.523002 7_3:+58843013 Msa1112870:CDS
GATCAACAAGGCCGCTGATG+AGG 0.531095 7_3:+58842804 Msa1112870:CDS
TGTGGGGTTAATAGGCCCGC+TGG 0.535503 7_3:-58842686 None:intergenic
GTGGAAGGAGGTGGTGTCGA+AGG 0.538378 7_3:+58843097 Msa1112870:CDS
CTAATATTCAACAAATTGAG+AGG 0.545252 7_3:+58842478 None:intergenic
GGTGTTGAAGGAGTATCAAT+AGG 0.557717 7_3:+58843136 Msa1112870:CDS
CCATTGGCAATGGCATGCAT+TGG 0.559911 7_3:+58842620 Msa1112870:five_prime_UTR
TGGTGTCGAAGGTGGTGGTG+TGG 0.563486 7_3:+58843078 Msa1112870:intron
GGTGTGGAAAGACATGGTGG+TGG 0.564359 7_3:+58843163 Msa1112870:CDS
GATGGAGAAGGAGGTGTCGA+AGG 0.568848 7_3:+58843025 Msa1112870:CDS
CTTAACATCAATGCTGACTT+GGG 0.569880 7_3:-58842725 None:intergenic
ATCAACAAGGCCGCTGATGA+GGG 0.570640 7_3:+58842805 Msa1112870:CDS
GGTGTGGAAGGAGTTATCAT+AGG 0.571036 7_3:+58842998 Msa1112870:CDS
CGTCTACATGTGAAACCAGC+GGG 0.571587 7_3:+58842671 Msa1112870:five_prime_UTR
GTACAGCAAGGATTGTTTCG+TGG 0.574623 7_3:+58842827 Msa1112870:CDS
AAAATGGGTTCAAAGGGTGT+TGG 0.576868 7_3:-58843268 None:intergenic
CCAATGCATGCCATTGCCAA+TGG 0.578740 7_3:-58842620 None:intergenic
ATAGGTGGTGATGGAGAAGG+AGG 0.582945 7_3:+58843016 Msa1112870:CDS
AGTATCAATAGGTGGTGGTG+TGG 0.587184 7_3:+58843147 Msa1112870:CDS
AAGATATCATGTATGAGCAA+TGG 0.587650 7_3:-58842643 None:intergenic
GGCGTGGAAGGAGGGGACGA+AGG 0.588177 7_3:+58842971 Msa1112870:CDS
CACAACTACATTCAATACGT+TGG 0.588634 7_3:+58842856 Msa1112870:CDS
GGGTAATAGGAACCGTCCAT+TGG 0.590809 7_3:+58842604 Msa1112870:five_prime_UTR
ATCGGTGGTGGGGTAGAAGG+AGG 0.598545 7_3:+58842929 Msa1112870:CDS
GAAGGAGTTACCACAGGTGG+TGG 0.599734 7_3:+58843115 Msa1112870:CDS
TAGGAACCGTCCATTGGCAA+TGG 0.601035 7_3:+58842610 Msa1112870:five_prime_UTR
TGCATGCCATTGCCAATGGA+CGG 0.604279 7_3:-58842616 None:intergenic
TCCTTCAACACCACCACCTG+TGG 0.606270 7_3:-58843125 None:intergenic
TAATATTCAACAAATTGAGA+GGG 0.610035 7_3:+58842479 None:intergenic
GGTGGTGTGGAAAGACATGG+TGG 0.619769 7_3:+58843160 Msa1112870:CDS
AATAATCTTGAACCGTTGTG+GGG 0.623368 7_3:-58842702 None:intergenic
GTGGAAGGAGTTATCATAGG+TGG 0.636745 7_3:+58843001 Msa1112870:CDS
GGTGTCGAAGGAGTTACCAC+AGG 0.637015 7_3:+58843109 Msa1112870:CDS
CGTAGGAATTGATGGTGGCG+TGG 0.652918 7_3:+58842955 Msa1112870:CDS
GGTGGTGGTGTGGAAAGACA+TGG 0.658421 7_3:+58843157 Msa1112870:CDS
GTCGAAGGAGTTACCACAGG+TGG 0.663904 7_3:+58843112 Msa1112870:CDS
ACGTCTACATGTGAAACCAG+CGG 0.672566 7_3:+58842670 Msa1112870:five_prime_UTR
CAGAGGCATCTAGATCAACA+AGG 0.676803 7_3:+58842792 Msa1112870:CDS
CCTTGCTGTACCCTCATCAG+CGG 0.683231 7_3:-58842815 None:intergenic
CCGCTGATGAGGGTACAGCA+AGG 0.684881 7_3:+58842815 Msa1112870:CDS
GAAGGAGTATCAATAGGTGG+TGG 0.692295 7_3:+58843142 Msa1112870:CDS
GTTGAAGGAGTATCAATAGG+TGG 0.722968 7_3:+58843139 Msa1112870:CDS
GGTGTCGTAGGAATTGATGG+TGG 0.735782 7_3:+58842950 Msa1112870:CDS
CGGGCCTATTAACCCCACAA+CGG 0.752886 7_3:+58842690 Msa1112870:five_prime_UTR
TGCAGCGCAAAGACACACAG+AGG 0.757164 7_3:+58842775 Msa1112870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGATTAGTATTATTATAA+TGG + chr7_3:58842544-58842563 Msa1112870:five_prime_UTR 10.0%
!! GTACATAATTAATTAATATA+GGG + chr7_3:58842584-58842603 Msa1112870:five_prime_UTR 10.0%
!! ATAGATTAGTATTATTATAA+TGG + chr7_3:58842544-58842563 Msa1112870:five_prime_UTR 10.0%
!! GTACATAATTAATTAATATA+GGG + chr7_3:58842584-58842603 Msa1112870:five_prime_UTR 10.0%
!! AATACTAATCTATATATTTG+TGG - chr7_3:58842537-58842556 None:intergenic 15.0%
!! GGTACATAATTAATTAATAT+AGG + chr7_3:58842583-58842602 Msa1112870:five_prime_UTR 15.0%
!! ATTAATTAATATAGGGTAAT+AGG + chr7_3:58842591-58842610 Msa1112870:five_prime_UTR 15.0%
!! AATACTAATCTATATATTTG+TGG - chr7_3:58842537-58842556 None:intergenic 15.0%
!! GGTACATAATTAATTAATAT+AGG + chr7_3:58842583-58842602 Msa1112870:five_prime_UTR 15.0%
!! ATTAATTAATATAGGGTAAT+AGG + chr7_3:58842591-58842610 Msa1112870:five_prime_UTR 15.0%
!! AATGGACGTTATTAATTTAT+AGG + chr7_3:58842562-58842581 Msa1112870:five_prime_UTR 20.0%
!!! TTTAAAAGGTTACAATTTTG+CGG + chr7_3:58842893-58842912 Msa1112870:CDS 20.0%
!! AATGGACGTTATTAATTTAT+AGG + chr7_3:58842562-58842581 Msa1112870:five_prime_UTR 20.0%
!!! TTTAAAAGGTTACAATTTTG+CGG + chr7_3:58842893-58842912 Msa1112870:CDS 20.0%
! GTTCTTAATTGTGATATCAA+TGG - chr7_3:58842507-58842526 None:intergenic 25.0%
!!! CGCAAATTTTCAAGTTTAAA+AGG + chr7_3:58842879-58842898 Msa1112870:CDS 25.0%
!! TTAAAAGGTTACAATTTTGC+GGG + chr7_3:58842894-58842913 Msa1112870:CDS 25.0%
! ATTAAGGAAGAGAATGAAAA+TGG + chr7_3:58843243-58843262 Msa1112870:three_prime_UTR 25.0%
! ATCAGCATTATTTGCAAAAT+GGG - chr7_3:58843286-58843305 None:intergenic 25.0%
! AATCAGCATTATTTGCAAAA+TGG - chr7_3:58843287-58843306 None:intergenic 25.0%
! GTTCTTAATTGTGATATCAA+TGG - chr7_3:58842507-58842526 None:intergenic 25.0%
!!! CGCAAATTTTCAAGTTTAAA+AGG + chr7_3:58842879-58842898 Msa1112870:CDS 25.0%
!! TTAAAAGGTTACAATTTTGC+GGG + chr7_3:58842894-58842913 Msa1112870:CDS 25.0%
! ATTAAGGAAGAGAATGAAAA+TGG + chr7_3:58843243-58843262 Msa1112870:three_prime_UTR 25.0%
! ATCAGCATTATTTGCAAAAT+GGG - chr7_3:58843286-58843305 None:intergenic 25.0%
! AATCAGCATTATTTGCAAAA+TGG - chr7_3:58843287-58843306 None:intergenic 25.0%
AAGATATCATGTATGAGCAA+TGG - chr7_3:58842646-58842665 None:intergenic 30.0%
! TAAAAGGTTACAATTTTGCG+GGG + chr7_3:58842895-58842914 Msa1112870:CDS 30.0%
! ATTTGCAAAATGGGTTCAAA+GGG - chr7_3:58843277-58843296 None:intergenic 30.0%
! TATTTGCAAAATGGGTTCAA+AGG - chr7_3:58843278-58843297 None:intergenic 30.0%
AAGATATCATGTATGAGCAA+TGG - chr7_3:58842646-58842665 None:intergenic 30.0%
! TAAAAGGTTACAATTTTGCG+GGG + chr7_3:58842895-58842914 Msa1112870:CDS 30.0%
! ATTTGCAAAATGGGTTCAAA+GGG - chr7_3:58843277-58843296 None:intergenic 30.0%
! TATTTGCAAAATGGGTTCAA+AGG - chr7_3:58843278-58843297 None:intergenic 30.0%
AATAATCTTGAACCGTTGTG+GGG - chr7_3:58842705-58842724 None:intergenic 35.0%
GAATAATCTTGAACCGTTGT+GGG - chr7_3:58842706-58842725 None:intergenic 35.0%
!! CTTAACATCAATGCTGACTT+GGG - chr7_3:58842728-58842747 None:intergenic 35.0%
! TCTTAACATCAATGCTGACT+TGG - chr7_3:58842729-58842748 None:intergenic 35.0%
CACAACTACATTCAATACGT+TGG + chr7_3:58842856-58842875 Msa1112870:CDS 35.0%
! AAAAGGTTACAATTTTGCGG+GGG + chr7_3:58842896-58842915 Msa1112870:CDS 35.0%
TAGAGACCAAAAGGAGATTA+AGG + chr7_3:58843227-58843246 Msa1112870:three_prime_UTR 35.0%
! TCTCTTCCTTAATCTCCTTT+TGG - chr7_3:58843236-58843255 None:intergenic 35.0%
AATAATCTTGAACCGTTGTG+GGG - chr7_3:58842705-58842724 None:intergenic 35.0%
GAATAATCTTGAACCGTTGT+GGG - chr7_3:58842706-58842725 None:intergenic 35.0%
!! CTTAACATCAATGCTGACTT+GGG - chr7_3:58842728-58842747 None:intergenic 35.0%
! TCTTAACATCAATGCTGACT+TGG - chr7_3:58842729-58842748 None:intergenic 35.0%
CACAACTACATTCAATACGT+TGG + chr7_3:58842856-58842875 Msa1112870:CDS 35.0%
! AAAAGGTTACAATTTTGCGG+GGG + chr7_3:58842896-58842915 Msa1112870:CDS 35.0%
TAGAGACCAAAAGGAGATTA+AGG + chr7_3:58843227-58843246 Msa1112870:three_prime_UTR 35.0%
! TCTCTTCCTTAATCTCCTTT+TGG - chr7_3:58843236-58843255 None:intergenic 35.0%
GGAATAATCTTGAACCGTTG+TGG - chr7_3:58842707-58842726 None:intergenic 40.0%
GCACTCAGAGCTAATATCTT+GGG - chr7_3:58842758-58842777 None:intergenic 40.0%
TGCACTCAGAGCTAATATCT+TGG - chr7_3:58842759-58842778 None:intergenic 40.0%
TACAGCAAGGATTGTTTCGT+GGG + chr7_3:58842828-58842847 Msa1112870:CDS 40.0%
!! GGTGTTGAAGGAGTATCAAT+AGG + chr7_3:58843136-58843155 Msa1112870:CDS 40.0%
!! GTTGAAGGAGTATCAATAGG+TGG + chr7_3:58843139-58843158 Msa1112870:CDS 40.0%
CAACAAGAGTAGAGACCAAA+AGG + chr7_3:58843218-58843237 Msa1112870:three_prime_UTR 40.0%
! AAAATGGGTTCAAAGGGTGT+TGG - chr7_3:58843271-58843290 None:intergenic 40.0%
GGAATAATCTTGAACCGTTG+TGG - chr7_3:58842707-58842726 None:intergenic 40.0%
GCACTCAGAGCTAATATCTT+GGG - chr7_3:58842758-58842777 None:intergenic 40.0%
TGCACTCAGAGCTAATATCT+TGG - chr7_3:58842759-58842778 None:intergenic 40.0%
TACAGCAAGGATTGTTTCGT+GGG + chr7_3:58842828-58842847 Msa1112870:CDS 40.0%
!! GGTGTTGAAGGAGTATCAAT+AGG + chr7_3:58843136-58843155 Msa1112870:CDS 40.0%
!! GTTGAAGGAGTATCAATAGG+TGG + chr7_3:58843139-58843158 Msa1112870:CDS 40.0%
CAACAAGAGTAGAGACCAAA+AGG + chr7_3:58843218-58843237 Msa1112870:three_prime_UTR 40.0%
! AAAATGGGTTCAAAGGGTGT+TGG - chr7_3:58843271-58843290 None:intergenic 40.0%
ACGTCTACATGTGAAACCAG+CGG + chr7_3:58842670-58842689 Msa1112870:five_prime_UTR 45.0%
TGAACCGTTGTGGGGTTAAT+AGG - chr7_3:58842697-58842716 None:intergenic 45.0%
!! CAGAGGCATCTAGATCAACA+AGG + chr7_3:58842792-58842811 Msa1112870:CDS 45.0%
GTACAGCAAGGATTGTTTCG+TGG + chr7_3:58842827-58842846 Msa1112870:CDS 45.0%
GGTGTGGAAGGAGTTATCAT+AGG + chr7_3:58842998-58843017 Msa1112870:CDS 45.0%
GTGGAAGGAGTTATCATAGG+TGG + chr7_3:58843001-58843020 Msa1112870:CDS 45.0%
GGAGTTATCATAGGTGGTGA+TGG + chr7_3:58843007-58843026 Msa1112870:CDS 45.0%
!! ATCATAGGTGGTGATGGAGA+AGG + chr7_3:58843013-58843032 Msa1112870:CDS 45.0%
! TGGTGTTGAAGGATCAACCA+CGG + chr7_3:58843051-58843070 Msa1112870:intron 45.0%
!! GAAGGAGTATCAATAGGTGG+TGG + chr7_3:58843142-58843161 Msa1112870:CDS 45.0%
! AGTATCAATAGGTGGTGGTG+TGG + chr7_3:58843147-58843166 Msa1112870:CDS 45.0%
ACGTCTACATGTGAAACCAG+CGG + chr7_3:58842670-58842689 Msa1112870:five_prime_UTR 45.0%
TGAACCGTTGTGGGGTTAAT+AGG - chr7_3:58842697-58842716 None:intergenic 45.0%
!! CAGAGGCATCTAGATCAACA+AGG + chr7_3:58842792-58842811 Msa1112870:CDS 45.0%
GTACAGCAAGGATTGTTTCG+TGG + chr7_3:58842827-58842846 Msa1112870:CDS 45.0%
GGTGTGGAAGGAGTTATCAT+AGG + chr7_3:58842998-58843017 Msa1112870:CDS 45.0%
GTGGAAGGAGTTATCATAGG+TGG + chr7_3:58843001-58843020 Msa1112870:CDS 45.0%
GGAGTTATCATAGGTGGTGA+TGG + chr7_3:58843007-58843026 Msa1112870:CDS 45.0%
!! ATCATAGGTGGTGATGGAGA+AGG + chr7_3:58843013-58843032 Msa1112870:CDS 45.0%
! TGGTGTTGAAGGATCAACCA+CGG + chr7_3:58843051-58843070 Msa1112870:intron 45.0%
!! GAAGGAGTATCAATAGGTGG+TGG + chr7_3:58843142-58843161 Msa1112870:CDS 45.0%
! AGTATCAATAGGTGGTGGTG+TGG + chr7_3:58843147-58843166 Msa1112870:CDS 45.0%
GGGTAATAGGAACCGTCCAT+TGG + chr7_3:58842604-58842623 Msa1112870:five_prime_UTR 50.0%
TAGGAACCGTCCATTGGCAA+TGG + chr7_3:58842610-58842629 Msa1112870:five_prime_UTR 50.0%
TGCATGCCATTGCCAATGGA+CGG - chr7_3:58842619-58842638 None:intergenic 50.0%
CCAATGCATGCCATTGCCAA+TGG - chr7_3:58842623-58842642 None:intergenic 50.0%
CCATTGGCAATGGCATGCAT+TGG + chr7_3:58842620-58842639 Msa1112870:five_prime_UTR 50.0%
CGTCTACATGTGAAACCAGC+GGG + chr7_3:58842671-58842690 Msa1112870:five_prime_UTR 50.0%
ATCAACAAGGCCGCTGATGA+GGG + chr7_3:58842805-58842824 Msa1112870:CDS 50.0%
!! GGAGGTGTCGTAGGAATTGA+TGG + chr7_3:58842947-58842966 Msa1112870:CDS 50.0%
! GGTGTCGTAGGAATTGATGG+TGG + chr7_3:58842950-58842969 Msa1112870:CDS 50.0%
!! ATAGGTGGTGATGGAGAAGG+AGG + chr7_3:58843016-58843035 Msa1112870:CDS 50.0%
! GGTGTTGAAGGATCAACCAC+GGG + chr7_3:58843052-58843071 Msa1112870:intron 50.0%
GGGTAATAGGAACCGTCCAT+TGG + chr7_3:58842604-58842623 Msa1112870:five_prime_UTR 50.0%
TAGGAACCGTCCATTGGCAA+TGG + chr7_3:58842610-58842629 Msa1112870:five_prime_UTR 50.0%
TGCATGCCATTGCCAATGGA+CGG - chr7_3:58842619-58842638 None:intergenic 50.0%
CCAATGCATGCCATTGCCAA+TGG - chr7_3:58842623-58842642 None:intergenic 50.0%
CCATTGGCAATGGCATGCAT+TGG + chr7_3:58842620-58842639 Msa1112870:five_prime_UTR 50.0%
CGTCTACATGTGAAACCAGC+GGG + chr7_3:58842671-58842690 Msa1112870:five_prime_UTR 50.0%
ATCAACAAGGCCGCTGATGA+GGG + chr7_3:58842805-58842824 Msa1112870:CDS 50.0%
!! GGAGGTGTCGTAGGAATTGA+TGG + chr7_3:58842947-58842966 Msa1112870:CDS 50.0%
! GGTGTCGTAGGAATTGATGG+TGG + chr7_3:58842950-58842969 Msa1112870:CDS 50.0%
!! ATAGGTGGTGATGGAGAAGG+AGG + chr7_3:58843016-58843035 Msa1112870:CDS 50.0%
! GGTGTTGAAGGATCAACCAC+GGG + chr7_3:58843052-58843071 Msa1112870:intron 50.0%
CGGGCCTATTAACCCCACAA+CGG + chr7_3:58842690-58842709 Msa1112870:five_prime_UTR 55.0%
TGCAGCGCAAAGACACACAG+AGG + chr7_3:58842775-58842794 Msa1112870:CDS 55.0%
GATCAACAAGGCCGCTGATG+AGG + chr7_3:58842804-58842823 Msa1112870:CDS 55.0%
! CCTTGCTGTACCCTCATCAG+CGG - chr7_3:58842818-58842837 None:intergenic 55.0%
!!! CAATTTTGCGGGGGTGTCAC+CGG + chr7_3:58842905-58842924 Msa1112870:CDS 55.0%
!! TTCTACCCCACCACCGATTC+CGG - chr7_3:58842927-58842946 None:intergenic 55.0%
!! CGTAGGAATTGATGGTGGCG+TGG + chr7_3:58842955-58842974 Msa1112870:CDS 55.0%
!! GGAATTGATGGTGGCGTGGA+AGG + chr7_3:58842959-58842978 Msa1112870:CDS 55.0%
!! ATTGATGGTGGCGTGGAAGG+AGG + chr7_3:58842962-58842981 Msa1112870:CDS 55.0%
!! TTGATGGTGGCGTGGAAGGA+GGG + chr7_3:58842963-58842982 Msa1112870:CDS 55.0%
!! GATGGAGAAGGAGGTGTCGA+AGG + chr7_3:58843025-58843044 Msa1112870:CDS 55.0%
! GTCGAAGGTGGTGGTGTTGA+AGG + chr7_3:58843040-58843059 Msa1112870:intron 55.0%
GTTGAAGGATCAACCACGGG+TGG + chr7_3:58843055-58843074 Msa1112870:intron 55.0%
! GGTGTCGAAGGAGTTACCAC+AGG + chr7_3:58843109-58843128 Msa1112870:CDS 55.0%
GTCGAAGGAGTTACCACAGG+TGG + chr7_3:58843112-58843131 Msa1112870:CDS 55.0%
GAAGGAGTTACCACAGGTGG+TGG + chr7_3:58843115-58843134 Msa1112870:CDS 55.0%
! ACCACAGGTGGTGGTGTTGA+AGG + chr7_3:58843124-58843143 Msa1112870:CDS 55.0%
TCCTTCAACACCACCACCTG+TGG - chr7_3:58843128-58843147 None:intergenic 55.0%
! GGTGGTGGTGTGGAAAGACA+TGG + chr7_3:58843157-58843176 Msa1112870:CDS 55.0%
GGTGGTGTGGAAAGACATGG+TGG + chr7_3:58843160-58843179 Msa1112870:CDS 55.0%
GGTGTGGAAAGACATGGTGG+TGG + chr7_3:58843163-58843182 Msa1112870:CDS 55.0%
!! GTGGAAAGACATGGTGGTGG+TGG + chr7_3:58843166-58843185 Msa1112870:CDS 55.0%
!! AGACATGGTGGTGGTGGTAG+TGG + chr7_3:58843172-58843191 Msa1112870:CDS 55.0%
CGGGCCTATTAACCCCACAA+CGG + chr7_3:58842690-58842709 Msa1112870:five_prime_UTR 55.0%
TGCAGCGCAAAGACACACAG+AGG + chr7_3:58842775-58842794 Msa1112870:CDS 55.0%
GATCAACAAGGCCGCTGATG+AGG + chr7_3:58842804-58842823 Msa1112870:CDS 55.0%
! CCTTGCTGTACCCTCATCAG+CGG - chr7_3:58842818-58842837 None:intergenic 55.0%
!!! CAATTTTGCGGGGGTGTCAC+CGG + chr7_3:58842905-58842924 Msa1112870:CDS 55.0%
!! TTCTACCCCACCACCGATTC+CGG - chr7_3:58842927-58842946 None:intergenic 55.0%
!! CGTAGGAATTGATGGTGGCG+TGG + chr7_3:58842955-58842974 Msa1112870:CDS 55.0%
!! GGAATTGATGGTGGCGTGGA+AGG + chr7_3:58842959-58842978 Msa1112870:CDS 55.0%
!! ATTGATGGTGGCGTGGAAGG+AGG + chr7_3:58842962-58842981 Msa1112870:CDS 55.0%
!! TTGATGGTGGCGTGGAAGGA+GGG + chr7_3:58842963-58842982 Msa1112870:CDS 55.0%
!! GATGGAGAAGGAGGTGTCGA+AGG + chr7_3:58843025-58843044 Msa1112870:CDS 55.0%
! GTCGAAGGTGGTGGTGTTGA+AGG + chr7_3:58843040-58843059 Msa1112870:intron 55.0%
GTTGAAGGATCAACCACGGG+TGG + chr7_3:58843055-58843074 Msa1112870:intron 55.0%
! GGTGTCGAAGGAGTTACCAC+AGG + chr7_3:58843109-58843128 Msa1112870:CDS 55.0%
GTCGAAGGAGTTACCACAGG+TGG + chr7_3:58843112-58843131 Msa1112870:CDS 55.0%
GAAGGAGTTACCACAGGTGG+TGG + chr7_3:58843115-58843134 Msa1112870:CDS 55.0%
! ACCACAGGTGGTGGTGTTGA+AGG + chr7_3:58843124-58843143 Msa1112870:CDS 55.0%
TCCTTCAACACCACCACCTG+TGG - chr7_3:58843128-58843147 None:intergenic 55.0%
! GGTGGTGGTGTGGAAAGACA+TGG + chr7_3:58843157-58843176 Msa1112870:CDS 55.0%
GGTGGTGTGGAAAGACATGG+TGG + chr7_3:58843160-58843179 Msa1112870:CDS 55.0%
GGTGTGGAAAGACATGGTGG+TGG + chr7_3:58843163-58843182 Msa1112870:CDS 55.0%
!! GTGGAAAGACATGGTGGTGG+TGG + chr7_3:58843166-58843185 Msa1112870:CDS 55.0%
!! AGACATGGTGGTGGTGGTAG+TGG + chr7_3:58843172-58843191 Msa1112870:CDS 55.0%
TGTGGGGTTAATAGGCCCGC+TGG - chr7_3:58842689-58842708 None:intergenic 60.0%
! CCGCTGATGAGGGTACAGCA+AGG + chr7_3:58842815-58842834 Msa1112870:CDS 60.0%
GTGTCACCGGAATCGGTGGT+GGG + chr7_3:58842918-58842937 Msa1112870:CDS 60.0%
TGTCACCGGAATCGGTGGTG+GGG + chr7_3:58842919-58842938 Msa1112870:CDS 60.0%
!! GGAATCGGTGGTGGGGTAGA+AGG + chr7_3:58842926-58842945 Msa1112870:CDS 60.0%
!! ATCGGTGGTGGGGTAGAAGG+AGG + chr7_3:58842929-58842948 Msa1112870:CDS 60.0%
!! GGGGTAGAAGGAGGTGTCGT+AGG + chr7_3:58842938-58842957 Msa1112870:CDS 60.0%
!! TGATGGTGGCGTGGAAGGAG+GGG + chr7_3:58842964-58842983 Msa1112870:CDS 60.0%
! GACGAAGGTGGAGGTGTGGA+AGG + chr7_3:58842986-58843005 Msa1112870:CDS 60.0%
!! GGAGAAGGAGGTGTCGAAGG+TGG + chr7_3:58843028-58843047 Msa1112870:intron 60.0%
!! GAAGGAGGTGTCGAAGGTGG+TGG + chr7_3:58843031-58843050 Msa1112870:intron 60.0%
GAAGGATCAACCACGGGTGG+TGG + chr7_3:58843058-58843077 Msa1112870:intron 60.0%
! TGGTGTCGAAGGTGGTGGTG+TGG + chr7_3:58843078-58843097 Msa1112870:intron 60.0%
! GTCGAAGGTGGTGGTGTGGA+AGG + chr7_3:58843082-58843101 Msa1112870:intron 60.0%
! GAAGGTGGTGGTGTGGAAGG+AGG + chr7_3:58843085-58843104 Msa1112870:intron 60.0%
! GTGGAAGGAGGTGGTGTCGA+AGG + chr7_3:58843097-58843116 Msa1112870:CDS 60.0%
TGTGGGGTTAATAGGCCCGC+TGG - chr7_3:58842689-58842708 None:intergenic 60.0%
! CCGCTGATGAGGGTACAGCA+AGG + chr7_3:58842815-58842834 Msa1112870:CDS 60.0%
GTGTCACCGGAATCGGTGGT+GGG + chr7_3:58842918-58842937 Msa1112870:CDS 60.0%
TGTCACCGGAATCGGTGGTG+GGG + chr7_3:58842919-58842938 Msa1112870:CDS 60.0%
!! GGAATCGGTGGTGGGGTAGA+AGG + chr7_3:58842926-58842945 Msa1112870:CDS 60.0%
!! ATCGGTGGTGGGGTAGAAGG+AGG + chr7_3:58842929-58842948 Msa1112870:CDS 60.0%
!! GGGGTAGAAGGAGGTGTCGT+AGG + chr7_3:58842938-58842957 Msa1112870:CDS 60.0%
!! TGATGGTGGCGTGGAAGGAG+GGG + chr7_3:58842964-58842983 Msa1112870:CDS 60.0%
! GACGAAGGTGGAGGTGTGGA+AGG + chr7_3:58842986-58843005 Msa1112870:CDS 60.0%
!! GGAGAAGGAGGTGTCGAAGG+TGG + chr7_3:58843028-58843047 Msa1112870:intron 60.0%
!! GAAGGAGGTGTCGAAGGTGG+TGG + chr7_3:58843031-58843050 Msa1112870:intron 60.0%
GAAGGATCAACCACGGGTGG+TGG + chr7_3:58843058-58843077 Msa1112870:intron 60.0%
! TGGTGTCGAAGGTGGTGGTG+TGG + chr7_3:58843078-58843097 Msa1112870:intron 60.0%
! GTCGAAGGTGGTGGTGTGGA+AGG + chr7_3:58843082-58843101 Msa1112870:intron 60.0%
! GAAGGTGGTGGTGTGGAAGG+AGG + chr7_3:58843085-58843104 Msa1112870:intron 60.0%
! GTGGAAGGAGGTGGTGTCGA+AGG + chr7_3:58843097-58843116 Msa1112870:CDS 60.0%
!! TGCGGGGGTGTCACCGGAAT+CGG + chr7_3:58842911-58842930 Msa1112870:CDS 65.0%
! GGTGTCACCGGAATCGGTGG+TGG + chr7_3:58842917-58842936 Msa1112870:CDS 65.0%
GTGGAAGGAGGGGACGAAGG+TGG + chr7_3:58842974-58842993 Msa1112870:CDS 65.0%
GAAGGAGGGGACGAAGGTGG+AGG + chr7_3:58842977-58842996 Msa1112870:CDS 65.0%
AGGGGACGAAGGTGGAGGTG+TGG + chr7_3:58842982-58843001 Msa1112870:CDS 65.0%
! ACCACGGGTGGTGGTGTCGA+AGG + chr7_3:58843067-58843086 Msa1112870:intron 65.0%
ACCTTCGACACCACCACCCG+TGG - chr7_3:58843071-58843090 None:intergenic 65.0%
! ACGGGTGGTGGTGTCGAAGG+TGG + chr7_3:58843070-58843089 Msa1112870:intron 65.0%
! GGTGGTGGTGTCGAAGGTGG+TGG + chr7_3:58843073-58843092 Msa1112870:intron 65.0%
! GGTGGTGGTGTGGAAGGAGG+TGG + chr7_3:58843088-58843107 Msa1112870:intron 65.0%
!! TGCGGGGGTGTCACCGGAAT+CGG + chr7_3:58842911-58842930 Msa1112870:CDS 65.0%
! GGTGTCACCGGAATCGGTGG+TGG + chr7_3:58842917-58842936 Msa1112870:CDS 65.0%
GTGGAAGGAGGGGACGAAGG+TGG + chr7_3:58842974-58842993 Msa1112870:CDS 65.0%
GAAGGAGGGGACGAAGGTGG+AGG + chr7_3:58842977-58842996 Msa1112870:CDS 65.0%
AGGGGACGAAGGTGGAGGTG+TGG + chr7_3:58842982-58843001 Msa1112870:CDS 65.0%
! ACCACGGGTGGTGGTGTCGA+AGG + chr7_3:58843067-58843086 Msa1112870:intron 65.0%
ACCTTCGACACCACCACCCG+TGG - chr7_3:58843071-58843090 None:intergenic 65.0%
! ACGGGTGGTGGTGTCGAAGG+TGG + chr7_3:58843070-58843089 Msa1112870:intron 65.0%
! GGTGGTGGTGTCGAAGGTGG+TGG + chr7_3:58843073-58843092 Msa1112870:intron 65.0%
! GGTGGTGGTGTGGAAGGAGG+TGG + chr7_3:58843088-58843107 Msa1112870:intron 65.0%
!! GGGGGTGTCACCGGAATCGG+TGG + chr7_3:58842914-58842933 Msa1112870:CDS 70.0%
GGCGTGGAAGGAGGGGACGA+AGG + chr7_3:58842971-58842990 Msa1112870:CDS 70.0%
!! GGGGGTGTCACCGGAATCGG+TGG + chr7_3:58842914-58842933 Msa1112870:CDS 70.0%
GGCGTGGAAGGAGGGGACGA+AGG + chr7_3:58842971-58842990 Msa1112870:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 58842491 58843330 58842491 ID=Msa1112870;Name=Msa1112870
chr7_3 mRNA 58842491 58843330 58842491 ID=Msa1112870-mRNA-1;Parent=Msa1112870;Name=Msa1112870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=249|1|1|1|0|0|2|120|142
chr7_3 exon 58842491 58843046 58842491 ID=Msa1112870-mRNA-1:exon:12987;Parent=Msa1112870-mRNA-1
chr7_3 exon 58843089 58843330 58843089 ID=Msa1112870-mRNA-1:exon:12988;Parent=Msa1112870-mRNA-1
chr7_3 five_prime_UTR 58842491 58842739 58842491 ID=Msa1112870-mRNA-1:five_prime_utr;Parent=Msa1112870-mRNA-1
chr7_3 CDS 58842740 58843046 58842740 ID=Msa1112870-mRNA-1:cds;Parent=Msa1112870-mRNA-1
chr7_3 CDS 58843089 58843210 58843089 ID=Msa1112870-mRNA-1:cds;Parent=Msa1112870-mRNA-1
chr7_3 three_prime_UTR 58843211 58843330 58843211 ID=Msa1112870-mRNA-1:three_prime_utr;Parent=Msa1112870-mRNA-1
Gene Sequence

>Msa1112870

ATGTTAAGACGTATTCCCAAGATATTAGCTCTGAGTGCAGCGCAAAGACACACAGAGGCATCTAGATCAACAAGGCCGCTGATGAGGGTACAGCAAGGATTGTTTCGTGGGTTGAACACAACTACATTCAATACGTTGGCGCAAATTTTCAAGTTTAAAAGGTTACAATTTTGCGGGGGTGTCACCGGAATCGGTGGTGGGGTAGAAGGAGGTGTCGTAGGAATTGATGGTGGCGTGGAAGGAGGGGACGAAGGTGGAGGTGTGGAAGGAGTTATCATAGGTGGTGATGGAGAAGGAGGTGTCGAAGGTGGTGGTGTGGAAGGAGGTGGTGTCGAAGGAGTTACCACAGGTGGTGGTGTTGAAGGAGTATCAATAGGTGGTGGTGTGGAAAGACATGGTGGTGGTGGTAGTGGTCTCGTAAGAGCATGA
Protein sequence

>Msa1112870

MLRRIPKILALSAAQRHTEASRSTRPLMRVQQGLFRGLNTTTFNTLAQIFKFKRLQFCGGVTGIGGGVEGGVVGIDGGVEGGDEGGGVEGVIIGGDGEGGVEGGGVEGGGVEGVTTGGGVEGVSIGGGVERHGGGGSGLVRA*