AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0311810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311810 MtrunA17_Chr2g0290571 74.879 207 44 4 1 206 1 200 2.27e-47 154
Msa0311810 MtrunA17_Chr2g0290561 74.879 207 40 3 1 206 1 196 9.05e-43 142
Msa0311810 MtrunA17_Chr1g0149481 44.776 134 54 4 74 204 47 163 4.43e-25 95.9
Msa0311810 MtrunA17_Chr2g0290531 50.617 81 38 1 126 204 179 259 2.54e-21 88.6
Msa0311810 MtrunA17_Chr4g0056371 48.718 78 35 2 131 204 55 131 6.74e-19 79.0
Msa0311810 MtrunA17_Chr4g0060391 47.436 78 36 2 131 204 46 122 5.47e-18 76.3
Msa0311810 MtrunA17_Chr1g0149471 45.238 84 41 2 126 204 126 209 7.04e-18 78.2
Msa0311810 MtrunA17_Chr4g0056411 50.633 79 35 1 131 205 91 169 7.38e-18 77.4
Msa0311810 MtrunA17_Chr2g0290521 50.649 77 34 2 131 204 51 126 3.18e-17 74.7
Msa0311810 MtrunA17_Chr4g0056391 52.564 78 33 1 131 204 65 142 3.38e-17 75.1
Msa0311810 MtrunA17_Chr4g0056401 50.000 78 35 1 131 204 56 133 1.03e-15 70.9
Msa0311810 MtrunA17_Chr3g0137521 46.154 78 38 2 131 204 52 129 2.71e-14 67.0
Msa0311810 MtrunA17_Chr1g0149491 35.714 112 54 3 98 204 52 150 5.31e-13 63.9
Msa0311810 MtrunA17_Chr3g0137531 50.943 53 26 0 152 204 76 128 9.14e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311810 AT5G46890.1 50.562 89 43 1 117 204 38 126 2.53e-23 90.9
Msa0311810 AT5G46900.1 54.667 75 33 1 131 204 52 126 2.09e-21 85.9
Msa0311810 AT4G12490.1 52.083 96 39 2 113 204 89 181 2.45e-21 87.0
Msa0311810 AT4G12480.1 56.410 78 30 1 131 204 90 167 2.55e-20 84.3
Msa0311810 AT4G12500.1 55.128 78 31 1 131 204 99 176 4.31e-20 84.0
Msa0311810 AT4G12470.1 53.846 78 32 1 131 204 83 160 2.06e-19 81.6
Msa0311810 AT4G12520.1 51.282 78 34 2 131 204 51 128 6.83e-19 79.3
Msa0311810 AT4G12510.1 51.282 78 34 2 131 204 51 128 6.83e-19 79.3
Msa0311810 AT2G45180.1 49.351 77 36 1 131 204 57 133 2.62e-18 77.8
Msa0311810 AT1G12100.1 41.667 96 52 1 113 204 36 131 1.38e-17 75.9
Msa0311810 AT4G12545.1 48.936 94 36 4 116 204 21 107 6.08e-17 73.6
Msa0311810 AT4G12550.1 42.857 112 56 4 98 204 2 110 1.09e-16 73.2
Msa0311810 AT1G62510.1 51.282 78 34 1 131 204 72 149 1.84e-16 73.6
Msa0311810 AT4G22460.1 43.617 94 46 3 113 202 40 130 1.59e-15 70.5
Msa0311810 AT4G00165.1 41.250 80 43 2 129 204 49 128 4.95e-14 66.6
Msa0311810 AT4G00165.2 41.250 80 43 2 129 204 49 128 4.95e-14 66.6
Msa0311810 AT4G12530.1 43.038 79 42 1 129 204 37 115 2.59e-13 64.3
Msa0311810 AT1G12090.1 40.909 110 45 2 99 204 44 137 2.59e-11 59.7

Find 71 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAAATGTTCAGGTTTAAA+AGG 0.204426 2_4:+6702406 None:intergenic
TCTCTTCCTTAATCTCCTTT+TGG 0.272655 2_4:-6702760 Msa0311810:CDS
GTGGAAGGAGGTGTGGTAGG+TGG 0.274444 2_4:+6702501 None:intergenic
TGGAAGGAGGTGTGGTAGGT+GGG 0.286221 2_4:+6702502 None:intergenic
ATTAAGGAAGAGAATGAAAA+TGG 0.290274 2_4:+6702770 None:intergenic
GGAAGGAGGTGTGGTAGGTG+GGG 0.297605 2_4:+6702503 None:intergenic
TAGAGACCAAAAGGAGATTA+AGG 0.298692 2_4:+6702754 None:intergenic
ATACGTTGGCGCAAATGTTC+AGG 0.359112 2_4:+6702397 None:intergenic
TGAACCGTTGTGGGCGTGAT+AGG 0.385447 2_4:-6702221 Msa0311810:CDS
GGTGGTGGTGTGGAAGGAGG+TGG 0.386380 2_4:+6702615 None:intergenic
GGTGCTGAAGGAGCTATAAT+AGG 0.409389 2_4:+6702540 None:intergenic
GAAGGAGGTGTGGTAGGTGG+GGG 0.411184 2_4:+6702504 None:intergenic
GGAGGTGTCGTAGGAATTGA+TGG 0.412771 2_4:+6702474 None:intergenic
AAACATGGTGATGGTGGTGG+TGG 0.429540 2_4:+6702699 None:intergenic
TTAACGACGACATACGAAAC+TGG 0.437551 2_4:+6702194 None:intergenic
CATGGTGATGGTGGTGGTGG+TGG 0.441525 2_4:+6702702 None:intergenic
GGTGTGGAAGGAGGTGTGGT+AGG 0.444236 2_4:+6702498 None:intergenic
GGAGTCGATGGTGGTGTAGA+AGG 0.446829 2_4:+6702453 None:intergenic
GTGGGTGGTGGTGTGGAAGG+AGG 0.451417 2_4:+6702612 None:intergenic
CAACAAGAGTAGAGACCAAA+AGG 0.469234 2_4:+6702745 None:intergenic
TGCACTCAGAGCTAATATCT+TGG 0.474145 2_4:-6702283 Msa0311810:CDS
GCACTCAGAGCTAATATCTT+GGG 0.475433 2_4:-6702282 Msa0311810:CDS
GAATAATCTTGAACCGTTGT+GGG 0.482405 2_4:-6702230 Msa0311810:CDS
ACCACAGGTGGTGGTGTTGA+AGG 0.497195 2_4:+6702651 None:intergenic
GGAGCTATAATAGGTGGTGA+TGG 0.499770 2_4:+6702549 None:intergenic
TCTTAACATCAATGCTGACT+TGG 0.513922 2_4:-6702253 Msa0311810:CDS
ACTGTGGGTGGTGGTGTGGA+AGG 0.518532 2_4:+6702609 None:intergenic
GGAATAATCTTGAACCGTTG+TGG 0.519169 2_4:-6702231 Msa0311810:CDS
GAAGGAGGTGTCGAAGGTGG+TGG 0.519674 2_4:+6702573 None:intergenic
GGTGTAGAAGGAGGTGTCGT+AGG 0.524763 2_4:+6702465 None:intergenic
TACAACAAGGATTTGTTCGT+GGG 0.526682 2_4:+6702355 None:intergenic
CCTTGTTGATCTAGATGCCT+CGG 0.532285 2_4:-6702319 Msa0311810:CDS
GTCGAAGGTGGAGGTGCTGA+AGG 0.535205 2_4:+6702528 None:intergenic
GGAATTGATGGTGGTGTGGA+AGG 0.536327 2_4:+6702486 None:intergenic
GTGGAAGGAGGTGGTGTCGA+AGG 0.538378 2_4:+6702624 None:intergenic
GTCGAAGGTGGTGGTGTCGA+AGG 0.546498 2_4:+6702582 None:intergenic
ATAATAGGTGGTGATGGAGA+AGG 0.546891 2_4:+6702555 None:intergenic
ATCAACTGTGGGTGGTGGTG+TGG 0.547443 2_4:+6702605 None:intergenic
TGGTGGTGTGGAAGGAGGTG+TGG 0.549904 2_4:+6702494 None:intergenic
CTACAACAAGGATTTGTTCG+TGG 0.565019 2_4:+6702354 None:intergenic
GGTGTTGAAGGAGTATGAAC+AGG 0.565828 2_4:+6702663 None:intergenic
GATGGAGAAGGAGGTGTCGA+AGG 0.568848 2_4:+6702567 None:intergenic
CTTAACATCAATGCTGACTT+GGG 0.569880 2_4:-6702252 Msa0311810:CDS
ATAGGTGGTGATGGAGAAGG+AGG 0.573916 2_4:+6702558 None:intergenic
GAAGGAGTAACCACAGGTGG+TGG 0.574063 2_4:+6702642 None:intergenic
ATTGATGGTGGTGTGGAAGG+AGG 0.574469 2_4:+6702489 None:intergenic
CGACGACATACGAAACTGGC+GGG 0.580767 2_4:+6702198 None:intergenic
GAAGGATCAACTGTGGGTGG+TGG 0.590571 2_4:+6702600 None:intergenic
GGAGAAGGAGGTGTCGAAGG+TGG 0.595365 2_4:+6702570 None:intergenic
AAAATGGGTTCAAAGTGTGT+TGG 0.604168 2_4:-6702795 Msa0311810:CDS
GATCAACAAGGCCACTGATG+AGG 0.608971 2_4:+6702331 None:intergenic
GAAGGAGTATGAACAGGTGG+TGG 0.609049 2_4:+6702669 None:intergenic
TCCTTCAACACCACCACCTG+TGG 0.611196 2_4:-6702652 Msa0311810:CDS
CGTAGGAATTGATGGTGGTG+TGG 0.614401 2_4:+6702482 None:intergenic
AGTATGAACAGGTGGTGGTG+TGG 0.619838 2_4:+6702674 None:intergenic
GGTGTCGTTGGAGTCGATGG+TGG 0.627154 2_4:+6702444 None:intergenic
GGTGTCGAAGGATCAACTGT+GGG 0.629264 2_4:+6702594 None:intergenic
GGTGTCGAAGGAGTAACCAC+AGG 0.637015 2_4:+6702636 None:intergenic
CCTTGTTGTAGCCTCATCAG+TGG 0.641265 2_4:-6702342 Msa0311810:CDS
GTCGAAGGATCAACTGTGGG+TGG 0.642906 2_4:+6702597 None:intergenic
GTCGATGGTGGTGTAGAAGG+AGG 0.650665 2_4:+6702456 None:intergenic
ACGACGACATACGAAACTGG+CGG 0.652934 2_4:+6702197 None:intergenic
GCTGAAGGAGCTATAATAGG+TGG 0.664529 2_4:+6702543 None:intergenic
CCACTGATGAGGCTACAACA+AGG 0.671797 2_4:+6702342 None:intergenic
CGGGCCTATCACGCCCACAA+CGG 0.674677 2_4:+6702217 None:intergenic
CCGAGGCATCTAGATCAACA+AGG 0.679099 2_4:+6702319 None:intergenic
GTCGAAGGAGTAACCACAGG+TGG 0.682488 2_4:+6702639 None:intergenic
GTTGAAGGAGTATGAACAGG+TGG 0.694706 2_4:+6702666 None:intergenic
TGGTGTCGAAGGATCAACTG+TGG 0.694920 2_4:+6702593 None:intergenic
GGTGTCGTAGGAATTGATGG+TGG 0.714173 2_4:+6702477 None:intergenic
TGCAGCACAAAGACACACCG+AGG 0.744286 2_4:+6702302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAAGGTTACAATTTTG+CGG + chr2_4:6702569-6702588 None:intergenic 20.0%
!! AAAAACTATATTCAATACGT+TGG + chr2_4:6702606-6702625 None:intergenic 20.0%
! ATTAAGGAAGAGAATGAAAA+TGG + chr2_4:6702219-6702238 None:intergenic 25.0%
!! TTAAAAGGTTACAATTTTGC+GGG + chr2_4:6702568-6702587 None:intergenic 25.0%
! TAAAAGGTTACAATTTTGCG+GGG + chr2_4:6702567-6702586 None:intergenic 30.0%
! TCTCTTCCTTAATCTCCTTT+TGG - chr2_4:6702226-6702245 Msa0311810:CDS 35.0%
TAGAGACCAAAAGGAGATTA+AGG + chr2_4:6702235-6702254 None:intergenic 35.0%
! AAAAGGTTACAATTTTGCGG+GGG + chr2_4:6702566-6702585 None:intergenic 35.0%
! CGCAAATGTTCAGGTTTAAA+AGG + chr2_4:6702583-6702602 None:intergenic 35.0%
! TACAACAAGGATTTGTTCGT+GGG + chr2_4:6702634-6702653 None:intergenic 35.0%
! TCTTAACATCAATGCTGACT+TGG - chr2_4:6702733-6702752 Msa0311810:CDS 35.0%
!! CTTAACATCAATGCTGACTT+GGG - chr2_4:6702734-6702753 Msa0311810:CDS 35.0%
GAATAATCTTGAACCGTTGT+GGG - chr2_4:6702756-6702775 Msa0311810:CDS 35.0%
CAACAAGAGTAGAGACCAAA+AGG + chr2_4:6702244-6702263 None:intergenic 40.0%
!! ATAATAGGTGGTGATGGAGA+AGG + chr2_4:6702434-6702453 None:intergenic 40.0%
! CTACAACAAGGATTTGTTCG+TGG + chr2_4:6702635-6702654 None:intergenic 40.0%
TGCACTCAGAGCTAATATCT+TGG - chr2_4:6702703-6702722 Msa0311810:CDS 40.0%
GCACTCAGAGCTAATATCTT+GGG - chr2_4:6702704-6702723 Msa0311810:CDS 40.0%
GGAATAATCTTGAACCGTTG+TGG - chr2_4:6702755-6702774 Msa0311810:CDS 40.0%
TTAACGACGACATACGAAAC+TGG + chr2_4:6702795-6702814 None:intergenic 40.0%
!! GAAAAACATGGTGATGGTGG+TGG + chr2_4:6702293-6702312 None:intergenic 45.0%
!! GTGGAAAAACATGGTGATGG+TGG + chr2_4:6702296-6702315 None:intergenic 45.0%
GGTGTGGAAAAACATGGTGA+TGG + chr2_4:6702299-6702318 None:intergenic 45.0%
! GTTGAAGGAGTATGAACAGG+TGG + chr2_4:6702323-6702342 None:intergenic 45.0%
! GGTGTTGAAGGAGTATGAAC+AGG + chr2_4:6702326-6702345 None:intergenic 45.0%
GGAGCTATAATAGGTGGTGA+TGG + chr2_4:6702440-6702459 None:intergenic 45.0%
GCTGAAGGAGCTATAATAGG+TGG + chr2_4:6702446-6702465 None:intergenic 45.0%
! GGTGCTGAAGGAGCTATAAT+AGG + chr2_4:6702449-6702468 None:intergenic 45.0%
ATACGTTGGCGCAAATGTTC+AGG + chr2_4:6702592-6702611 None:intergenic 45.0%
CCTTGTTGATCTAGATGCCT+CGG - chr2_4:6702667-6702686 Msa0311810:CDS 45.0%
!! AAACATGGTGATGGTGGTGG+TGG + chr2_4:6702290-6702309 None:intergenic 50.0%
! GGTGGTGGTGTGGAAAAACA+TGG + chr2_4:6702305-6702324 None:intergenic 50.0%
! AGTATGAACAGGTGGTGGTG+TGG + chr2_4:6702315-6702334 None:intergenic 50.0%
! GAAGGAGTATGAACAGGTGG+TGG + chr2_4:6702320-6702339 None:intergenic 50.0%
! GGTGTCGAAGGATCAACTGT+GGG + chr2_4:6702395-6702414 None:intergenic 50.0%
! TGGTGTCGAAGGATCAACTG+TGG + chr2_4:6702396-6702415 None:intergenic 50.0%
!! ATAGGTGGTGATGGAGAAGG+AGG + chr2_4:6702431-6702450 None:intergenic 50.0%
!! ATTGATGGTGGTGTGGAAGG+AGG + chr2_4:6702500-6702519 None:intergenic 50.0%
!! GGAATTGATGGTGGTGTGGA+AGG + chr2_4:6702503-6702522 None:intergenic 50.0%
!! CGTAGGAATTGATGGTGGTG+TGG + chr2_4:6702507-6702526 None:intergenic 50.0%
! GGTGTCGTAGGAATTGATGG+TGG + chr2_4:6702512-6702531 None:intergenic 50.0%
!! GGAGGTGTCGTAGGAATTGA+TGG + chr2_4:6702515-6702534 None:intergenic 50.0%
CCACTGATGAGGCTACAACA+AGG + chr2_4:6702647-6702666 None:intergenic 50.0%
! CCTTGTTGTAGCCTCATCAG+TGG - chr2_4:6702644-6702663 Msa0311810:CDS 50.0%
GATCAACAAGGCCACTGATG+AGG + chr2_4:6702658-6702677 None:intergenic 50.0%
! CCGAGGCATCTAGATCAACA+AGG + chr2_4:6702670-6702689 None:intergenic 50.0%
ACGACGACATACGAAACTGG+CGG + chr2_4:6702792-6702811 None:intergenic 50.0%
!! GATGGTGGTGGTGGTGGTAA+AGG + chr2_4:6702281-6702300 None:intergenic 55.0%
TCCTTCAACACCACCACCTG+TGG - chr2_4:6702334-6702353 Msa0311810:CDS 55.0%
! ACCACAGGTGGTGGTGTTGA+AGG + chr2_4:6702338-6702357 None:intergenic 55.0%
GAAGGAGTAACCACAGGTGG+TGG + chr2_4:6702347-6702366 None:intergenic 55.0%
GTCGAAGGAGTAACCACAGG+TGG + chr2_4:6702350-6702369 None:intergenic 55.0%
! GGTGTCGAAGGAGTAACCAC+AGG + chr2_4:6702353-6702372 None:intergenic 55.0%
! ATCAACTGTGGGTGGTGGTG+TGG + chr2_4:6702384-6702403 None:intergenic 55.0%
GAAGGATCAACTGTGGGTGG+TGG + chr2_4:6702389-6702408 None:intergenic 55.0%
GTCGAAGGATCAACTGTGGG+TGG + chr2_4:6702392-6702411 None:intergenic 55.0%
!! GATGGAGAAGGAGGTGTCGA+AGG + chr2_4:6702422-6702441 None:intergenic 55.0%
! TGGAAGGAGGTGTGGTAGGT+GGG + chr2_4:6702487-6702506 None:intergenic 55.0%
!! GGTGTAGAAGGAGGTGTCGT+AGG + chr2_4:6702524-6702543 None:intergenic 55.0%
! GTCGATGGTGGTGTAGAAGG+AGG + chr2_4:6702533-6702552 None:intergenic 55.0%
! GGAGTCGATGGTGGTGTAGA+AGG + chr2_4:6702536-6702555 None:intergenic 55.0%
!!! CAATTTTGCGGGGGTGTCGT+TGG + chr2_4:6702557-6702576 None:intergenic 55.0%
TGCAGCACAAAGACACACCG+AGG + chr2_4:6702687-6702706 None:intergenic 55.0%
TGAACCGTTGTGGGCGTGAT+AGG - chr2_4:6702765-6702784 Msa0311810:CDS 55.0%
CGACGACATACGAAACTGGC+GGG + chr2_4:6702791-6702810 None:intergenic 55.0%
!! CATGGTGATGGTGGTGGTGG+TGG + chr2_4:6702287-6702306 None:intergenic 60.0%
! GTGGAAGGAGGTGGTGTCGA+AGG + chr2_4:6702365-6702384 None:intergenic 60.0%
! ACTGTGGGTGGTGGTGTGGA+AGG + chr2_4:6702380-6702399 None:intergenic 60.0%
! GTCGAAGGTGGTGGTGTCGA+AGG + chr2_4:6702407-6702426 None:intergenic 60.0%
!! GAAGGAGGTGTCGAAGGTGG+TGG + chr2_4:6702416-6702435 None:intergenic 60.0%
!! GGAGAAGGAGGTGTCGAAGG+TGG + chr2_4:6702419-6702438 None:intergenic 60.0%
!! GTCGAAGGTGGAGGTGCTGA+AGG + chr2_4:6702461-6702480 None:intergenic 60.0%
! GAAGGAGGTGTGGTAGGTGG+GGG + chr2_4:6702485-6702504 None:intergenic 60.0%
! GGAAGGAGGTGTGGTAGGTG+GGG + chr2_4:6702486-6702505 None:intergenic 60.0%
! GTGGAAGGAGGTGTGGTAGG+TGG + chr2_4:6702488-6702507 None:intergenic 60.0%
! GGTGTGGAAGGAGGTGTGGT+AGG + chr2_4:6702491-6702510 None:intergenic 60.0%
! TGGTGGTGTGGAAGGAGGTG+TGG + chr2_4:6702372-6702391 None:intergenic 60.0%
!! GGTGTCGTTGGAGTCGATGG+TGG + chr2_4:6702545-6702564 None:intergenic 60.0%
! GGTGGTGGTGTGGAAGGAGG+TGG + chr2_4:6702374-6702393 None:intergenic 65.0%
! GTGGGTGGTGGTGTGGAAGG+AGG + chr2_4:6702377-6702396 None:intergenic 65.0%
!! GTAGGTGGGGGTGTCGAAGG+TGG + chr2_4:6702473-6702492 None:intergenic 65.0%
!! GTGGTAGGTGGGGGTGTCGA+AGG + chr2_4:6702476-6702495 None:intergenic 65.0%
!! GGGGGTGTCGTTGGAGTCGA+TGG + chr2_4:6702548-6702567 None:intergenic 65.0%
CGGGCCTATCACGCCCACAA+CGG + chr2_4:6702772-6702791 None:intergenic 65.0%
!! GGTGGGGGTGTCGAAGGTGG+AGG + chr2_4:6702470-6702489 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 6702194 6702814 6702194 ID=Msa0311810;Name=Msa0311810
chr2_4 mRNA 6702194 6702814 6702194 ID=Msa0311810-mRNA-1;Parent=Msa0311810;Name=Msa0311810-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|206
chr2_4 exon 6702194 6702814 6702194 ID=Msa0311810-mRNA-1:exon:2609;Parent=Msa0311810-mRNA-1
chr2_4 CDS 6702194 6702814 6702194 ID=Msa0311810-mRNA-1:cds;Parent=Msa0311810-mRNA-1
Gene Sequence

>Msa0311810

ATGGGTTCAAAGTGTGTTGGATCCATTTTCATTCTCTTCCTTAATCTCCTTTTGGTCTCTACTCTTGTTGTTGCAAGTCATGTTCCTTTACCACCACCACCACCATCACCATGTTTTTCCACACCACCACCTGTTCATACTCCTTCAACACCACCACCTGTGGTTACTCCTTCGACACCACCTCCTTCCACACCACCACCCACAGTTGATCCTTCGACACCACCACCTTCGACACCTCCTTCTCCATCACCACCTATTATAGCTCCTTCAGCACCTCCACCTTCGACACCCCCACCTACCACACCTCCTTCCACACCACCATCAATTCCTACGACACCTCCTTCTACACCACCATCGACTCCAACGACACCCCCGCAAAATTGTAACCTTTTAAACCTGAACATTTGCGCCAACGTATTGAATATAGTTTTTATCAACCCACGAACAAATCCTTGTTGTAGCCTCATCAGTGGCCTTGTTGATCTAGATGCCTCGGTGTGTCTTTGTGCTGCACTCAGAGCTAATATCTTGGGAATACGTCTTAACATCAATGCTGACTTGGGAATAATCTTGAACCGTTGTGGGCGTGATAGGCCCGCCAGTTTCGTATGTCGTCGTTAA
Protein sequence

>Msa0311810

MGSKCVGSIFILFLNLLLVSTLVVASHVPLPPPPPSPCFSTPPPVHTPSTPPPVVTPSTPPPSTPPPTVDPSTPPPSTPPSPSPPIIAPSAPPPSTPPPTTPPSTPPSIPTTPPSTPPSTPTTPPQNCNLLNLNICANVLNIVFINPRTNPCCSLISGLVDLDASVCLCAALRANILGIRLNINADLGIILNRCGRDRPASFVCRR*